Journal of biophotonics最新文献

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Correction to "Toward Informative Representations of Blood-Based Infrared Spectra via Unsupervised Deep Learning". 更正“通过无监督深度学习实现基于血液的红外光谱的信息表示”。

IF 2.3

Journal of biophotonics

Pub Date : 2025-11-06 DOI: 10.1002/jbio.70172

引用次数: 0

Magnetic-Plasmonic Au@Fe₃O₄ Nanostars Induced Non-Apoptotic Cell Death During Photothermal Therapy. 磁等离子体Au@Fe3O4纳米星在光热治疗期间诱导非凋亡细胞死亡。

IF 2.3

Journal of biophotonics

Pub Date : 2025-11-06 DOI: 10.1002/jbio.202500397

S E Pshenichnikov, A A Anikin, A V Motorzhina, M Albino, V V Malashchenko, L S Litvinova, V V Rodionova, C Sangregorio, L V Panina, K V Levada

In this study, we present our previously fabricated star-shaped magnetic-plasmonic Au@Fe₃O₄ nanostars as promising agents for photothermal therapy. The nanostars exhibit a photothermal conversion efficiency of ~60% at a concentration of 25 μg/mL under 808 nm laser irradiation. WST-1 analysis revealed that Au@Fe₃O₄ nanostars moderately reduced the viability of human hepatocarcinoma Huh7 cells after 24 h exposure at concentrations of 1, 5, and 10 μg/mL, accompanied by notable morphological alterations. Flow cytometry demonstrated that treatment with 5 μg/mL nanostars followed by 20 min of laser irradiation resulted in 79% elimination of cancer cells. Furthermore, photothermal therapy increased cellular granularity, with highly granulated cells comprising 23% of the population compared to 4% in untreated controls. The viability of these highly granulated cells decreased to 17% post-treatment. Interestingly, photothermal therapy reduced the proportion of apoptotic cells among Huh7 subpopulations relative to the overall number of dead cells.

在这项研究中，我们提出了我们以前制造的星形磁等离子体Au@Fe3O4纳米星作为光热治疗的有前途的药物。在808 nm激光照射下，纳米星在25 μg/mL的浓度下光热转换效率为~60%。WST-1分析显示，Au@Fe3O4纳米星在1、5和10 μg/mL浓度下暴露24 h后，中度降低人肝癌Huh7细胞的活力，并伴有明显的形态学改变。流式细胞术显示，5 μg/mL纳米星治疗后，激光照射20 min，癌细胞消除率为79%。此外，光热疗法增加了细胞粒度，高度颗粒化的细胞占总数的23%，而未经治疗的对照组为4%。这些高度颗粒化细胞的存活率在处理后下降到17%。有趣的是，相对于死亡细胞的总数，光热疗法降低了Huh7亚群中凋亡细胞的比例。

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引用次数: 0

Fast Mid-Infrared Spectral Probe Decisions Match H&E Stain Results for Keratinocytic Carcinoma. 快速中红外光谱探针决定匹配H&E染色结果角化细胞癌。

IF 2.3

Journal of biophotonics

Pub Date : 2025-11-04 DOI: 10.1002/jbio.202500311

Rebecca C Bradley, Maria G Vazquez de Vasquez, Charles L Hitchcock, Angela S Casey, James V Coe, Ronald Siegle

We designed a handheld and fast mid-infrared fiber-optic spectral probe using a quantum cascade laser (QCL) and a reduced range of wavelengths, to see if keratinocytic carcinoma (KC) could be distinguished from adjacent nonmalignant tissue using discarded skin tissues from Mohs surgery. This study employed two adjacent frozen sections of discarded tissue: one was stained with H&E (the gold standard for skin cancer diagnosis) to identify the location of cancer by a pathologist, while the other was left unstained for mid-infrared spectral probing on and off cancer as guided by the adjacent H&E stain. A total of 346 spectra from 18 consenting patients were collected during Mohs surgery. After adding a dehumidifier, an accuracy of 95% was obtained on a case sample basis. It will be worthwhile to assess the probe's utility at the surface of live human skin (study approved by the Advarra Institutional Review Board [PRO00044823]).

我们设计了一种手持式快速中红外光纤光谱探针，使用量子级联激光器（QCL）和缩小的波长范围，观察角化细胞癌（KC）是否可以从莫氏手术后丢弃的皮肤组织中区分出来。本研究使用两个相邻的冷冻丢弃组织切片：一个用H&E染色（皮肤癌诊断的金标准），由病理学家确定癌症的位置，而另一个不染色，在相邻的H&E染色的指导下，用于中红外光谱探测癌症的开启和关闭。在莫氏手术期间，共收集了18名同意患者的346个光谱。在添加除湿机后，在案例样本的基础上获得了95%的准确性。评估探针在活体皮肤表面的效用是值得的（这项研究已被Advarra机构审查委员会批准[PRO00044823]）。

引用次数: 0

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