Hao Guo, Vanessa S Zions, Brent A Law, Kevin C Hewitt
This study explores a combined strategy of Raman and reflectance spectroscopy for quantifying liver fat content and fat droplet size, crucial in assessing donor livers. By using Monte Carlo simulations and experimental setups with oil-in-water phantoms, our findings indicate that Raman scattering can solely differentiate between varying fat contents. At the same time, reflectance intensity is influenced by both fat content and oil droplet size, with a more pronounced sensitivity to fat droplet size. This study demonstrates the efficacy of combined Raman and reflectance spectroscopy in assessing liver steatosis and fat droplet size, potentially aiding in assessing donor livers for transplantation.
{"title":"Potential of Raman-Reflectance Combination in Quantifying Liver Steatosis and Fat Droplet Size: Evidence From Monte Carlo Simulations and Phantom Studies.","authors":"Hao Guo, Vanessa S Zions, Brent A Law, Kevin C Hewitt","doi":"10.1002/jbio.202400156","DOIUrl":"https://doi.org/10.1002/jbio.202400156","url":null,"abstract":"<p><p>This study explores a combined strategy of Raman and reflectance spectroscopy for quantifying liver fat content and fat droplet size, crucial in assessing donor livers. By using Monte Carlo simulations and experimental setups with oil-in-water phantoms, our findings indicate that Raman scattering can solely differentiate between varying fat contents. At the same time, reflectance intensity is influenced by both fat content and oil droplet size, with a more pronounced sensitivity to fat droplet size. This study demonstrates the efficacy of combined Raman and reflectance spectroscopy in assessing liver steatosis and fat droplet size, potentially aiding in assessing donor livers for transplantation.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multiphoton fluorescence microscopy excited with femtosecond pulses at high repetition rates, particularly in the range of 100's MHz to GHz, offers an alternative solution to suppress photoinduced damage to biological samples, for example, photobleaching. Here, we demonstrate the use of a U-Net-based deep-learning algorithm for suppressing the inherent shot noise of the two-photon fluorescence images excited with GHz femtosecond pulses. With the trained denoising neural network, the image quality of the representative two-photon fluorescence images of the biological samples is shown to be significantly improved. Moreover, for input raw images with even SNR reduced to -4.76 dB, the trained denoising network can recover the main image structure from noise floor with acceptable fidelity and spatial resolution. It is anticipated that the combination of GHz femtosecond pulses and deep-learning denoising algorithm can be a promising solution for eliminating the trade-off between photoinduced damage and image quality in nonlinear optical imaging platforms.
{"title":"Shot-Noise Limited Nonlinear Optical Imaging Excited With GHz Femtosecond Pulses and Denoised by Deep-Learning.","authors":"Wenlong Wang, Junpeng Wen, Yuke Sheng, Chiyi Wei, Cihang Kong, Yalong Liu, Xiaoming Wei, Zhongmin Yang","doi":"10.1002/jbio.202400186","DOIUrl":"https://doi.org/10.1002/jbio.202400186","url":null,"abstract":"<p><p>Multiphoton fluorescence microscopy excited with femtosecond pulses at high repetition rates, particularly in the range of 100's MHz to GHz, offers an alternative solution to suppress photoinduced damage to biological samples, for example, photobleaching. Here, we demonstrate the use of a U-Net-based deep-learning algorithm for suppressing the inherent shot noise of the two-photon fluorescence images excited with GHz femtosecond pulses. With the trained denoising neural network, the image quality of the representative two-photon fluorescence images of the biological samples is shown to be significantly improved. Moreover, for input raw images with even SNR reduced to -4.76 dB, the trained denoising network can recover the main image structure from noise floor with acceptable fidelity and spatial resolution. It is anticipated that the combination of GHz femtosecond pulses and deep-learning denoising algorithm can be a promising solution for eliminating the trade-off between photoinduced damage and image quality in nonlinear optical imaging platforms.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Low back pain (LBP) is the leading cause of disability worldwide, yet its quantitative and noninvasive assessment remains challenging. Considering that near-infrared spectroscopy (NIRS) became a promising noninvasive tool for monitoring muscle and cupping therapy could regulate muscle blood flow to relieve LBP, we attempted to incorporate cupping and hemodynamics monitoring in muscle tissue by NIRS to assess LBP. We collected 3-min NIRS recordings on 12 LBP patients and 12 healthy subjects before and after 20-min cupping. Initially, no significant hemodynamic differences were observed between the groups. After cupping, the concentration changes of oxy-hemoglobin (Δ[HbO2]) in the emitter-detector channel parallel to spine unexpectedly exhibited that LBP was remarkably lower by approximately 67% compared with the controls. This study highlighted the potential of combining NIRS and cupping protocol as a quantitative assessment technique for LBP, also providing a new idea for clinical integration of novel optical assessment technologies.
{"title":"Noninvasive Assessing Low Back Pain by a Novel Near-Infrared Spectroscopy Flexible Probe With the Aid of Cupping Protocol.","authors":"Bowen Zhang, Yu Lin, Bo Yao, Ting Li","doi":"10.1002/jbio.202400204","DOIUrl":"https://doi.org/10.1002/jbio.202400204","url":null,"abstract":"<p><p>Low back pain (LBP) is the leading cause of disability worldwide, yet its quantitative and noninvasive assessment remains challenging. Considering that near-infrared spectroscopy (NIRS) became a promising noninvasive tool for monitoring muscle and cupping therapy could regulate muscle blood flow to relieve LBP, we attempted to incorporate cupping and hemodynamics monitoring in muscle tissue by NIRS to assess LBP. We collected 3-min NIRS recordings on 12 LBP patients and 12 healthy subjects before and after 20-min cupping. Initially, no significant hemodynamic differences were observed between the groups. After cupping, the concentration changes of oxy-hemoglobin (Δ[HbO<sub>2</sub>]) in the emitter-detector channel parallel to spine unexpectedly exhibited that LBP was remarkably lower by approximately 67% compared with the controls. This study highlighted the potential of combining NIRS and cupping protocol as a quantitative assessment technique for LBP, also providing a new idea for clinical integration of novel optical assessment technologies.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Postoperative bleeding is the most significant complication of tonsillectomy. Regular monitoring of post-surgical wound healing in the pharynx is required. For this purpose, we propose endoscope-based non-invasive perfusion mapping and quantification. The combination of imaging photoplethysmography and image processing provides automated wound area selection and microcirculation characterization. In this feasibility study, we demonstrate the first results of the proposed approach to wound monitoring in clinical trial on eight patients after tonsillectomy. Combination of probe-based optical system and image processing algorithms can provide the valuable and consistent data on perfusion distribution. The quantitative microcirculation data obtained 1, 4, and 7 days after surgery are in good agreement with existing monitoring protocols.
{"title":"Post-Surgical Non-Invasive Wound Healing Monitoring in Oropharyngeal Mucosa.","authors":"Anastasia Guryleva, Alexander Machikhin, Alexey Toldanov, Yevgeniya Kulikova, Demid Khokhlov, Anastasia Zolotukhina, Mikhail Svistushkin, Valeriy Svistushkin","doi":"10.1002/jbio.202400248","DOIUrl":"https://doi.org/10.1002/jbio.202400248","url":null,"abstract":"<p><p>Postoperative bleeding is the most significant complication of tonsillectomy. Regular monitoring of post-surgical wound healing in the pharynx is required. For this purpose, we propose endoscope-based non-invasive perfusion mapping and quantification. The combination of imaging photoplethysmography and image processing provides automated wound area selection and microcirculation characterization. In this feasibility study, we demonstrate the first results of the proposed approach to wound monitoring in clinical trial on eight patients after tonsillectomy. Combination of probe-based optical system and image processing algorithms can provide the valuable and consistent data on perfusion distribution. The quantitative microcirculation data obtained 1, 4, and 7 days after surgery are in good agreement with existing monitoring protocols.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehmet Ozcan, Ayse Burus, Etkin Boynuyogun, Mert Calis, Figen Ozgur, Yasemin Bayazit
Photobiomodulation (PBM) has emerged as a potentially effective therapeutic approach to modulate cellular functions. This study aimed to examine the impact of PBM on reactive oxygen species (ROS), lipid peroxidation, and glutathione-related antioxidant defense systems in rabbit eye tissues. A polychromatic light source with an intensity of 2.6 J/cm2/min was used for PBM treatment in New Zealand White rabbits for 12 min. The PBM group (n = 8) received treatments every 2 days for a total of 12 sessions, whereas the control group (n = 8) did not undergo any PBM light exposure during the same period. The application of PBM significantly elevated ROS-mediated glutathione levels, along with increased activities of glutathione peroxidase and reductase, particularly in corneal tissue (p ≤ 0.05). In conclusion, PBM treatment effectively enhances antioxidant defense mechanisms in the eye, particularly in corneal tissue, suggesting its potential as a therapeutic strategy for managing oxidative stress-related ocular conditions.
{"title":"Effects of Photobiomodulation Application on Glutathione-Related Antioxidant Defense System in Rabbit Eye Tissues.","authors":"Mehmet Ozcan, Ayse Burus, Etkin Boynuyogun, Mert Calis, Figen Ozgur, Yasemin Bayazit","doi":"10.1002/jbio.202400261","DOIUrl":"https://doi.org/10.1002/jbio.202400261","url":null,"abstract":"<p><p>Photobiomodulation (PBM) has emerged as a potentially effective therapeutic approach to modulate cellular functions. This study aimed to examine the impact of PBM on reactive oxygen species (ROS), lipid peroxidation, and glutathione-related antioxidant defense systems in rabbit eye tissues. A polychromatic light source with an intensity of 2.6 J/cm<sup>2</sup>/min was used for PBM treatment in New Zealand White rabbits for 12 min. The PBM group (n = 8) received treatments every 2 days for a total of 12 sessions, whereas the control group (n = 8) did not undergo any PBM light exposure during the same period. The application of PBM significantly elevated ROS-mediated glutathione levels, along with increased activities of glutathione peroxidase and reductase, particularly in corneal tissue (p ≤ 0.05). In conclusion, PBM treatment effectively enhances antioxidant defense mechanisms in the eye, particularly in corneal tissue, suggesting its potential as a therapeutic strategy for managing oxidative stress-related ocular conditions.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Photobiomodulation therapy, as an emerging treatment modality, has been widely used in dentistry. However, reports on blue light therapy for oral cancer are scarce. This study investigated the effects of 457 and 475 nm LED irradiation on SCC-25 cells and explored the potential mechanisms underlying the impact of blue light. Both wavelengths were found to inhibit cell viability, induce oxidative stress, and cause cell cycle arrest without leading to cell death. Notably, the inhibitory effect of 457 nm blue light on cell proliferation was more sustained. Transcriptome sequencing was performed to explore the underlying mechanisms, revealing that blue light induced endoplasmic reticulum stress in SCC-25 cells, with 457 nm light showing a more pronounced effect. Moreover, 457 nm blue light upregulated the expression of the aryl hydrocarbon receptor pathway, indicating potential therapeutic prospects for the combined use of blue light and pharmacological agents.
{"title":"Induction of Endoplasmic Reticulum Stress and Aryl Hydrocarbon Receptor Pathway Expression by Blue LED Irradiation in Oral Squamous Cell Carcinoma.","authors":"Hui Jiang, Jiali Yang, Qiqi Fu, Angze Li, Haokuan Qin, Muqing Liu","doi":"10.1002/jbio.202400226","DOIUrl":"https://doi.org/10.1002/jbio.202400226","url":null,"abstract":"<p><p>Photobiomodulation therapy, as an emerging treatment modality, has been widely used in dentistry. However, reports on blue light therapy for oral cancer are scarce. This study investigated the effects of 457 and 475 nm LED irradiation on SCC-25 cells and explored the potential mechanisms underlying the impact of blue light. Both wavelengths were found to inhibit cell viability, induce oxidative stress, and cause cell cycle arrest without leading to cell death. Notably, the inhibitory effect of 457 nm blue light on cell proliferation was more sustained. Transcriptome sequencing was performed to explore the underlying mechanisms, revealing that blue light induced endoplasmic reticulum stress in SCC-25 cells, with 457 nm light showing a more pronounced effect. Moreover, 457 nm blue light upregulated the expression of the aryl hydrocarbon receptor pathway, indicating potential therapeutic prospects for the combined use of blue light and pharmacological agents.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142116497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ekaterina Ryzhkova, Tatyana Morgunova, Elena Potapova, Ivan Ryzhkov, Valentin Fadeyev
The fluorescence intensities of the cellular respiratory cofactors NADH (reduced nicotinamide adenine dinucleotide) and FAD++ (oxidized flavin adenine dinucleotide) reflect energy metabolism in skin and other tissues and can be quantified in vivo by fluorescence spectroscopy (FS). However, the variability of physiological parameters largely determines the reproducibility of measurement results and the reliability of the diagnostic test. In this prospective study, we evaluated the interday reproducibility of NADH and FAD++ fluorescence intensity measurements in the skin of 51 healthy volunteers assessed by the FS at baseline, after local cooling (10°C) and heating of the skin (35°C). Results showed that the fluorescence amplitude of NADH (AFNADH) in forearm skin was the most reproducible of the FS parameters studied. Assessment of AFNADH in the dorsal forearm in combination with a thermal functional test is the most promising method for clinical use for assessing energy metabolism in the skin.
{"title":"Fluorescence Spectroscopy With Temperature Functional Tests in the Assessment of Markers of Intracellular Energy Metabolism: Spatial Heterogeneity and Reproducibility of Measurements.","authors":"Ekaterina Ryzhkova, Tatyana Morgunova, Elena Potapova, Ivan Ryzhkov, Valentin Fadeyev","doi":"10.1002/jbio.202400294","DOIUrl":"https://doi.org/10.1002/jbio.202400294","url":null,"abstract":"<p><p>The fluorescence intensities of the cellular respiratory cofactors NADH (reduced nicotinamide adenine dinucleotide) and FAD<sup>++</sup> (oxidized flavin adenine dinucleotide) reflect energy metabolism in skin and other tissues and can be quantified in vivo by fluorescence spectroscopy (FS). However, the variability of physiological parameters largely determines the reproducibility of measurement results and the reliability of the diagnostic test. In this prospective study, we evaluated the interday reproducibility of NADH and FAD<sup>++</sup> fluorescence intensity measurements in the skin of 51 healthy volunteers assessed by the FS at baseline, after local cooling (10°C) and heating of the skin (35°C). Results showed that the fluorescence amplitude of NADH (AF<sub>NADH</sub>) in forearm skin was the most reproducible of the FS parameters studied. Assessment of AF<sub>NADH</sub> in the dorsal forearm in combination with a thermal functional test is the most promising method for clinical use for assessing energy metabolism in the skin.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142094334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Optical coherence tomography angiography (OCTA), a functional extension of optical coherence tomography (OCT), is widely employed for high-resolution imaging of microvascular networks. However, due to the relatively low scan rate of OCT, the artifacts caused by the involuntary bulk motion of tissues severely impact the visualization of microvascular networks. This study proposes a fast motion correction method based on image feature matching for OCT microvascular images. First, the rigid motion-related mismatch between B-scans is compensated through the image feature matching based on the improved oriented FAST and rotated BRIEF algorithm. Then, the axial motion within A-scan lines in each B-scan image is corrected according to the displacement deviation between the detected boundaries achieved by the Scharr operator in a non-rigid transformation manner. Finally, an optimized intensity-based Doppler variance algorithm is developed to enhance the robustness of the OCTA imaging. The experimental results demonstrate the effectiveness of the method.
光学相干断层血管成像(OCTA)是光学相干断层成像(OCT)的功能扩展,被广泛用于微血管网络的高分辨率成像。然而,由于 OCT 的扫描速率相对较低,组织的不自主运动造成的伪影严重影响了微血管网络的可视化。本研究提出了一种基于图像特征匹配的 OCT 微血管图像快速运动校正方法。首先,基于改进的定向 FAST 和旋转 BRIEF 算法,通过图像特征匹配补偿 B 扫描之间与刚性运动相关的不匹配。然后,根据 Scharr 算子以非刚性变换方式实现的检测边界之间的位移偏差,校正每个 B 扫描图像中 A 扫描线内的轴向运动。最后,开发了一种基于强度的多普勒方差优化算法,以增强 OCTA 成像的鲁棒性。实验结果证明了该方法的有效性。
{"title":"Motion Artifact Correction for OCT Microvascular Images Based on Image Feature Matching.","authors":"Xudong Chen, Zongqing Ma, Chongyang Wang, Jiaqi Cui, Fan Fan, Xinxiao Gao, Jiang Zhu","doi":"10.1002/jbio.202400198","DOIUrl":"https://doi.org/10.1002/jbio.202400198","url":null,"abstract":"<p><p>Optical coherence tomography angiography (OCTA), a functional extension of optical coherence tomography (OCT), is widely employed for high-resolution imaging of microvascular networks. However, due to the relatively low scan rate of OCT, the artifacts caused by the involuntary bulk motion of tissues severely impact the visualization of microvascular networks. This study proposes a fast motion correction method based on image feature matching for OCT microvascular images. First, the rigid motion-related mismatch between B-scans is compensated through the image feature matching based on the improved oriented FAST and rotated BRIEF algorithm. Then, the axial motion within A-scan lines in each B-scan image is corrected according to the displacement deviation between the detected boundaries achieved by the Scharr operator in a non-rigid transformation manner. Finally, an optimized intensity-based Doppler variance algorithm is developed to enhance the robustness of the OCTA imaging. The experimental results demonstrate the effectiveness of the method.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142094335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qingdong Zhang, Peng Liu, Pengfei Shao, Mingzhai Sun, Peng Yao, Shuwei Shen, Yang Zhang, Ming Wu, Ronald X Xu
Pressure ulcers present a significant human and economic challenge, lacking a reliable method for early detection. To address this, we developed a system capable of early detection by using cooling stimulation and dynamic data acquisition techniques to monitor blood perfusion and skin temperature. The system consists of laser speckle perfusion imaging and thermal imaging. And we performed simulations to demonstrate that the system is capable of detect tissue damage across multiple layers, from superficial to deep. Testing on a rabbit ear model demonstrated that this approach, which combines dynamic perfusion and temperature parameters, effectively distinguishes early pressure ulcer areas from normal skin with a significant p value of 0.0015. This distinction was more precise compared to methods relying solely on static parameters or one parameter. Our study thereby offers a promising advancement in the proactive management and prevention of pressure ulcers.
由于缺乏早期检测的可靠方法,褥疮给人类和经济带来了巨大挑战。为了解决这个问题,我们开发了一种能够利用冷却刺激和动态数据采集技术监测血液灌注和皮肤温度的早期检测系统。该系统由激光斑点灌注成像和热成像组成。我们进行了模拟实验,证明该系统能够检测从浅层到深层的多层组织损伤。在兔耳模型上进行的测试表明,这种方法结合了动态灌注和温度参数,能有效区分早期压疮区域和正常皮肤,其显著的 p 值为 0.0015。与仅依靠静态参数或一种参数的方法相比,这种区分更为精确。因此,我们的研究为压疮的主动管理和预防带来了希望。
{"title":"Dual-Modal Optical Imaging of Tissue Perfusion in Response to Cooling Stimulation Facilitates Early Detection of Pressure Ulcer.","authors":"Qingdong Zhang, Peng Liu, Pengfei Shao, Mingzhai Sun, Peng Yao, Shuwei Shen, Yang Zhang, Ming Wu, Ronald X Xu","doi":"10.1002/jbio.202400188","DOIUrl":"https://doi.org/10.1002/jbio.202400188","url":null,"abstract":"<p><p>Pressure ulcers present a significant human and economic challenge, lacking a reliable method for early detection. To address this, we developed a system capable of early detection by using cooling stimulation and dynamic data acquisition techniques to monitor blood perfusion and skin temperature. The system consists of laser speckle perfusion imaging and thermal imaging. And we performed simulations to demonstrate that the system is capable of detect tissue damage across multiple layers, from superficial to deep. Testing on a rabbit ear model demonstrated that this approach, which combines dynamic perfusion and temperature parameters, effectively distinguishes early pressure ulcer areas from normal skin with a significant p value of 0.0015. This distinction was more precise compared to methods relying solely on static parameters or one parameter. Our study thereby offers a promising advancement in the proactive management and prevention of pressure ulcers.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhiying Xie, Yaping Shi, Agathe Marmin, Ruikang K Wang
Optical coherence tomography (OCT) is a noninvasive 3D imaging technique that offers significant advantages over traditional microscopy and biopsy in measuring epidermal thickness (ET) when assessing skin conditions. However, OCT imagining is often required to be in a contact mode for mitigating the issues of subject movement and uneven skin topology. It is not known whether the contact would affect the ability of ET measurements. In this study, we investigate the relationship between the contact pressure applied and the ET measurements. We observed progressive deformation in the epidermis with the increase of compression forces, where a notable decrease of up to 13% in ET measurement and 70% decrease in capillary vessels was noted when imaging was in contact mode. We also observed 8.1% less deformation properties in scar tissue than in nearby healthy tissue. Our study underscored the importance of controlled pressure in contact imaging mode, which is often neglected.
光学相干断层扫描(OCT)是一种无创三维成像技术,与传统的显微镜和活组织检查相比,它在评估皮肤状况时测量表皮厚度(ET)方面具有显著优势。不过,OCT 成像通常需要在接触模式下进行,以减少受试者移动和皮肤拓扑不均匀的问题。目前还不清楚接触模式是否会影响 ET 测量的能力。在本研究中,我们研究了接触压力与 ET 测量之间的关系。我们观察到表皮随着压力的增加而逐渐变形,在接触模式下成像时,ET 测量值明显下降达 13%,毛细血管下降 70%。我们还观察到疤痕组织的变形特性比附近的健康组织低 8.1%。我们的研究强调了在接触成像模式下控制压力的重要性,而这一点往往被忽视。
{"title":"Investigation of the Effect of Compression Pressure in Contact OCT Imaging on the Measurement of Epidermis Thickness.","authors":"Zhiying Xie, Yaping Shi, Agathe Marmin, Ruikang K Wang","doi":"10.1002/jbio.202400289","DOIUrl":"https://doi.org/10.1002/jbio.202400289","url":null,"abstract":"<p><p>Optical coherence tomography (OCT) is a noninvasive 3D imaging technique that offers significant advantages over traditional microscopy and biopsy in measuring epidermal thickness (ET) when assessing skin conditions. However, OCT imagining is often required to be in a contact mode for mitigating the issues of subject movement and uneven skin topology. It is not known whether the contact would affect the ability of ET measurements. In this study, we investigate the relationship between the contact pressure applied and the ET measurements. We observed progressive deformation in the epidermis with the increase of compression forces, where a notable decrease of up to 13% in ET measurement and 70% decrease in capillary vessels was noted when imaging was in contact mode. We also observed 8.1% less deformation properties in scar tissue than in nearby healthy tissue. Our study underscored the importance of controlled pressure in contact imaging mode, which is often neglected.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}