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Study on the fourof Pfaffia glomerata based on the metabolomics technology and comparison of Dangshen () in the equivalent substitution prescription. 基于代谢组学技术的团扇蕨研究及等效替代处方中丹参()的比较。
Pub Date : 2024-08-01 DOI: 10.19852/j.cnki.jtcm.20240521.001
Song Zhenguang, Yang Bin, Wang Fei, Yan Dongmei, Zhou Xiaoqing, Huang Liping, Gao Xuemei, L I Bin, Huang Luqi

Objective: To explore the four Qiof Pfaffia glomerata (PG) and endow this foreign folk herb with the properties of Chinese medicine, make it Chinese medicinal and localized, and could be used as a Chinese medicine.

Methods: The normal group, six cold herb groups, six hot herb groups, six cool herb groups, PG prescription group (PGPG), and Dangshen (Radix Codonopsis) prescription group (CPPG) were prepared with corresponding concentrations of water extracts, these herb extracts were administered by gavage to Sprague-Dawley rats, and the 12 h urine at night on the 29th day of the SD rats in each group were collected, Liquid Chromatograph Mass Spectrometer system was used to analyze them, the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up, so as to the medicinal properties of PGPG, CPPG and PG were predicted. Based on the Progenesis QI, Human Metabolome Database, Kyoto Encyclopedia of Genes and Genomes, MetaboAnalyst 5.0 database, we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG prescription.

Results: We established a best model of cold-hot herbal discrimination in the positive ion mode, then the probability that PGPG was predicted cold property was 88.9%. Furthermore, a model of cold-cool herbal discrimination was established, then the probability of PGPG containing the cool property was 77.8%. In addition, typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline, (R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in rats. The regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine metabolism.

Conclusion: The results showed PGPG had a cool medicinal property as same as CPPG, and the regulation trend of PGPG on markers was consistent with cool herbs. Therefore, the medicine properties PG and CP should be consistent, and the Traditional Chinese Medicine property of PG was predicted to be neutral.

目的方法:通过对四物汤、六味地黄汤、六味地黄汤、附子处方组(PGPG)、党参处方组(CPPG)的研究,探讨四物汤的药理作用:方法:用相应浓度的水提取物配制正常组、六味寒性药组、六味热性药组、六味凉性药组、PG处方组(PGPG)和党参处方组(CPPG),给Sprague-Dawley大鼠灌胃、收集各组 SD 大鼠第 29 天夜间 12 小时的尿液,用液相色谱质谱系统进行分析,建立寒热、寒凉药性的最佳判别模型,从而预测 PGPG、CPPG 和 PG 的药性。基于Progenesis QI、人类代谢组数据库、京都基因和基因组百科全书、MetaboAnalyst 5.0数据库,我们丰富了寒凉性中药的代谢途径和药性分类机制,以及PG处方的分子机制:结果:我们建立了正离子模式下冷热中药鉴别的最佳模型,该模型预测 PGPG 具有寒性的概率为 88.9%。此外,我们还建立了寒凉药材鉴别模型,PGPG 含有寒凉属性的概率为 77.8%。此外,典型的寒凉药材主要影响大鼠体内的酪氨酸-脯氨酸、(R)-3',7-二羟基-2',4'-二甲氧基异黄烷等九种生物标志物。PGPG对标志物的调节趋势与凉性药材基本一致,主要参与调节细胞色素P450和嘌呤代谢两条途径:结果表明,PGPG与CPPG一样具有清凉的药性,且PGPG对指标的调节趋势与清凉药材一致。因此,PGG 和 CP 的药性应该是一致的,并预测 PGG 的中药属性为中性。
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引用次数: 0
Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve. 艾灸对三味子苷诱发卵巢储备功能下降的 miRNAs 表达谱的影响
Pub Date : 2024-08-01 DOI: 10.19852/j.cnki.jtcm.2024.04.002
Shen Jie, Yin Yaoli, L I Hongxiao, L U Ge, Zhu Yaoyao, Qin Yantong, Jin Xun, Cheng Jie, Shen Meihong

Objective: To explore the possible regulatory mechanism of microRNA (miRNA) in moxibustion treatment for decreased ovarian reserve (DOR).

Methods: The DOR model was constructed by intragastrical Tripterygium glycoside suspension administration, and moxibustion therapy was simultaneously given. The morphological ovarian changes were observed by hematoxylin and eosin staining. The miRNA expression profile was detected by RNA sequencing, and bioinformatics analysis was performed. Cytoscape software 3.6.1 was used to establish a regulatory network and differentially expressed miRNAs were verified by reverse transcription quantitative polymerase chain reaction (RT-qPCR).

Results: Decreased number of mature follicles, increased atresia follicles, and abnormal granulosa cell morphology were observed in the model group compared with the control group. The moxibustion group demonstrated increased mature follicles, decreased atretic follicles, and significantly decreased abnormal morphology of granulosa cells compared with the model group. Additionally, RNA sequencing results manifested significantly up-regulated miRNA expressions (miR-92b-3p, miR-26-5p_R + 1_1ss10TC, miR-206-3p, miR-9993b-3p_1ss6GA, miR-7857-3p_R-1, miR-219a-2-3p_1ss10GC, miR-3968-p5_1ss10AT, and PC-5p-6478_1795) and down-regulated miR-664-2-5p_R + 1 in the model group, compared with the control group, and the moxibustion group reversed abnormal disorder levels of these miRNAs. Moreover, these differentially expressed miRNAs were mainly involved in the phosphatidylinositol-3-kinase / protein kinase B signaling pathway and nuclear factor erythropoietin-2-related factor 2 / heme oxygenase 1 signaling pathway. Finally, network and RT-qPCR verification revealed miR-9993b-3p_1ss6GA as the most critical miRNA.

Conclusion: This experiment proved the effectiveness of moxibustion in improving the ovarian reserve of rats by regulating miRNA expression, especially miR-9993b-3p_1ss6GA.

目的探讨微RNA(miRNA)在艾灸治疗卵巢储备功能下降(DOR)中的可能调控机制:方法:通过胃内注射三味子苷混悬液建立 DOR 模型,同时进行艾灸治疗。苏木精和伊红染色观察卵巢形态学变化。通过 RNA 测序检测 miRNA 表达谱,并进行生物信息学分析。使用 Cytoscape 软件 3.6.1 建立调控网络,并通过反转录定量聚合酶链反应(RT-qPCR)验证差异表达的 miRNA:结果:与对照组相比,模型组的成熟卵泡数量减少,闭锁卵泡增加,颗粒细胞形态异常。与模型组相比,艾灸组成熟卵泡增多,闭锁卵泡减少,颗粒细胞异常形态明显减少。此外,RNA 测序结果显示 miRNA 表达明显上调(miR-92b-3p、miR-26-5p_R + 1_1ss10TC、miR-206-3p、miR-9993b-3p_1ss6GA、miR-7857-3p_R-1、miR-219a-2-3p_1ss10GC、与对照组相比,模型组的 miR-3968-p5_1ss10AT 和 PC-5p-6478_1795)和 miR-664-2-5p_R + 1 下调,而艾灸组则逆转了这些 miRNA 的异常紊乱水平。此外,这些差异表达的miRNA主要参与磷脂酰肌醇-3-激酶/蛋白激酶B信号通路和核因子促红细胞生成素-2相关因子2/血红素加氧酶1信号通路。最后,通过网络和 RT-qPCR 验证,发现 miR-9993b-3p_1ss6GA 是最关键的 miRNA:本实验证明了艾灸通过调节 miRNA(尤其是 miR-9993b-3p_1ss6GA)的表达来改善大鼠卵巢储备功能的有效性。
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引用次数: 0
A real-world study of the differences in Traditional Chinese Medicine diagnosis and treatment rules for coronavirus disease 2019 between Northern and Southern China. 2019年冠状病毒病南北方中医诊疗规律差异的实证研究。
Pub Date : 2024-08-01 DOI: 10.19852/j.cnki.jtcm.2024.04.004
S U Rui, S U Youzhu, Wang Shuo, Fan Jie, Liu Qingquan, Liu Mifeng

Objective: To explore the differences in Traditional Chinese Medicine (TCM) diagnosis and treatment rules for coronavirus disease 2019 (COVID-19) between Northern and Southern China based on the real-world data from 982 COVID-19 patients.

Methods: All consecutive cases of COVID-19 admitted to the TCM department of designated COVID-19 hospitals in eight provinces and cities were retrospectively analyzed. Patients were divided into a Northern and a Southern group according to the location of the admitting hospital. The symptoms, syndrome elements, syndrome distribution and herbal treatments were analyzed. The core prescriptions were extracted using the multiscale backbone-based network comparison algorithm (msbNC).

Results: The distribution of syndrome elements showed that dampness was common in Northern and Southern China, wind and heat were more often present in the South, while fire toxin and spleen deficiency were more often encountered in the North. The distribution of syndromes showed that the South was dominated by heat dampness accumulating in the lung (55.69%), while the North was dominated by dampness-toxin stagnating in the lung (44.90%).The results of core prescription mining showed that dispelling dampness, dispersing wind, clearing heat and strengthening spleen were the common treatment methods in Northern and Southern China. For mild cases, Jinyinhua (Flos Lonicerae) and Lianqiao (Fructus Forsythiae Suspensae) were often used in the South to clear heat and relieve exterior symptoms, while Chaihu (Radix Bupleuri Chinensis) and Huangqin (Radix Scutellariae Baicalensis) were often used in the North to relieve muscles by expelling heat. For moderate cases, Chaihu (Radix Bupleuri Chinensis), Qinghao (Herba Artemisiae Annuae), and Shigao (Gypsum Fibrosum) were often used to clear heat of Tri-jiao Channel and stomach in the South, while Fuling (Poria), Chenpi (Pericarpium Citri Reticulatae), and Dangshen (Radix Codonopsis) were often used to invigorate spleen and remove dampness in the North. For severe cases, spleen invigoration and dampness removal as well as relaxing the bowels and discharging heat were often used in the North.

Conclusion: There were certain North-South differences in terms of symptoms, syndrome elements and syndrome distribution of COVID-19, as well as differences in core prescriptions during different periods of the disease. The regional differences in the rules of TCM diagnosis and treatment for COVID-19 should be further considered in the process of optimization and revision of relevant treatment guidance.

目的根据 982 例 COVID-19 患者的真实数据,探讨中国北方和南方在冠状病毒病 2019(COVID-19)的中医诊疗规则方面的差异:方法:对8个省市COVID-19定点医院中医科收治的所有COVID-19连续病例进行回顾性分析。根据收治医院所在地将患者分为北方组和南方组。分析了症状、证候要素、证候分布和中药治疗。使用基于多尺度骨干网络比较算法(msbNC)提取核心处方:证候分布显示,湿邪常见于中国北方和南方,风热多见于南方,而火毒和脾虚多见于北方。证候分布显示,南方以湿热蕴肺为主(55.69%),北方以湿毒壅肺为主(44.90%)。核心方药挖掘结果显示,祛湿、散风、清热、健脾是南北方常用的治疗方法。对于轻度病例,南方常用金银花、连翘清热解表,北方常用柴胡、黄芩祛热舒筋。对于中度病例,南方常用柴胡、秦艽、石膏清三焦胃热,北方常用茯苓、陈皮、党参健脾利湿。对于重症患者,北方常用健脾利湿、通便泄热的方法:结论:COVID-19 的症状、证候要素和证候分布存在一定的南北差异,不同时期的核心方药也存在差异。在优化和修订相关治疗指南的过程中,应进一步考虑 COVID-19 中医辨证论治规律的地区差异。
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引用次数: 0
Optimized new Shengmai powder inhibits myocardial fibrosis in heart failure by regulating the rat sarcoma/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway. 优化后的新圣麦粉通过调节大鼠肉瘤/快速加速纤维肉瘤/介原激活蛋白激酶激酶/胞外调控蛋白激酶信号通路,抑制心衰患者的心肌纤维化。
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.20240402.004
Zhang Zeyu, Jia Zhuangzhuang, Song Yuwei, Zhang Xuan, Wang Ci, Wang Shuai, Zhang Peipei, Ren Qiuan, Wang Xianliang, Mao Jingyuan

Objective: Exploring the effect of Optimized New Shengmai powder (, ONSMP) on myocardial fibrosis in heart failure (HF) based on rat sarcoma (RAS)/rapidly accelerated fibrosarcoma (RAF)/mitogen-activated protein kinase kinase (MEK)/extracellular regulated protein kinases (ERK) signaling pathway.

Methods: Randomized 70 Sprague-Dawley rats into sham (n = 10) and operation (n = 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low (L), medium (M), and high (H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen (COL) I and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the mRNA levels of COL I, COL Ⅲ, α-smooth muscle actin (α-SMA), and c-Fos proto-oncogene (c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor (p-ELK1), p-c-Fos, α-SMA, COL I, and COL Ⅲ by Western blot.

Results: ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL I/Ⅲ content, down-regulate the mRNA of COL I/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/ 2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA.

Conclusions: ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.

目的方法:将70只Sprague-Dawley大鼠随机分为假组(n=10)和手术组(n=60),通过结扎大鼠左前降支建立心衰大鼠的心肌纤维化:将 70 只 Sprague-Dawley 大鼠随机分为假手术组(n = 10)和手术组(n = 60),然后通过结扎冠状动脉左前降支建立高频大鼠。我们将手术组大鼠随机分为模型组、ONSMP(包括低剂量(L)、中剂量(M)和高剂量(H))组和依那普利组。在为期 4 周的药物干预后,超声心动图检查心脏功能,并计算全心/左心与大鼠体重的比率。最后,我们通过病理切片观察心肌纤维化的程度,用酶联免疫吸附法测定心肌胶原蛋白(COL)Ⅰ和COLⅢ的含量,检测COLⅠ、COLⅢ、α-平滑肌肌动蛋白(α-SMA)的mRNA水平、并通过Western blot检测p-RAS、p-RAF、p-MEK1/2、p-ERK1/2、p-ETS-like-1转录因子(p-ELK1)、p-c-Fos、α-SMA、COLⅠ和COLⅢ的蛋白表达。结果ONSMP能有效改善HF大鼠的心功能,降低心器官系数、COL体积分数和COLⅠ/Ⅲ含量,下调COLⅠ/Ⅲ、α-SMA和c-Fos的mRNA,以及p-RAS、p-RAF、p-MEK1/ 2、p-ERK1/2、p-ELK1、c-Fos、COLⅠ/Ⅲ和α-SMA的蛋白:结论:ONSMP能有效减轻高频大鼠的心肌纤维化,其机制可能与抑制RAS/RAF/MEK/ERK信号通路有关。
{"title":"Optimized new Shengmai powder inhibits myocardial fibrosis in heart failure by regulating the rat sarcoma/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase kinase/extracellular regulated protein kinases signaling pathway.","authors":"Zhang Zeyu, Jia Zhuangzhuang, Song Yuwei, Zhang Xuan, Wang Ci, Wang Shuai, Zhang Peipei, Ren Qiuan, Wang Xianliang, Mao Jingyuan","doi":"10.19852/j.cnki.jtcm.20240402.004","DOIUrl":"10.19852/j.cnki.jtcm.20240402.004","url":null,"abstract":"<p><strong>Objective: </strong>Exploring the effect of Optimized New Shengmai powder (, ONSMP) on myocardial fibrosis in heart failure (HF) based on rat sarcoma (RAS)/rapidly accelerated fibrosarcoma (RAF)/mitogen-activated protein kinase kinase (MEK)/extracellular regulated protein kinases (ERK) signaling pathway.</p><p><strong>Methods: </strong>Randomized 70 Sprague-Dawley rats into sham (<i>n =</i> 10) and operation (<i>n =</i> 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low (L), medium (M), and high (H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen (COL) I and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the mRNA levels of COL I, COL Ⅲ, α-smooth muscle actin (α-SMA), and c-Fos proto-oncogene (c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor (p-ELK1), p-c-Fos, α-SMA, COL I, and COL Ⅲ by Western blot.</p><p><strong>Results: </strong>ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL I/Ⅲ content, down-regulate the mRNA of COL I/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/ 2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA.</p><p><strong>Conclusions: </strong>ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"448-457"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phytochemical analysis, antioxidant, acetylcholinesterase, and α-amylase inhibitors from extract. 提取物中的植物化学分析、抗氧化剂、乙酰胆碱酯酶和α-淀粉酶抑制剂。
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.2024.03.001
Naila Sher, Mushtaq Ahmed, Nadia Mushtaq

Objective: To investigate the effects of Hippeastrum hybridum (HH) as a free radical scavenger, and an inhibitor of the two enzymes i-e Alpha-amylase (α-amylase) and acetylcholinesterase (AChE).

Methods: In this study, HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant, anti-α-amylase, and anti-AChE efficiency via standard procedures.

Results: Phytochemical analysis shows the existence of different compounds; while Coumarins and quinones were absent. The total phenolic, flavonoid, and tannins content were found to be (78.52 ± 0.69) mg GAE/g, (2.01 ± 0.04) mg RUE/g, and (58.12 ± 0.23) mg TAE/g of plant extract respectively. 28.02% ± 0.02% alkaloid and 2.02% ± 0.05% saponins were present in the HH extract. The HH extract showed the anti-oxidant property with IC50 (50% inhibition) of (151.01 ± 0.13) (HH), (79.01 ± 0.04) (Ascorbic acid) for ferric reducing, (91.48 ± 0.13) (HH), (48.02 ± 0.11) (Ascorbic acid) against Ammonium molybdenum, (156.02 ± 0.31) (HH), (52.38 ± 0.21) (Ascorbic acid) against DPPH, 136.01 ± 0.21 (HH), 52.02± 0.31 (Ascorbic acid) against H2O2, and 154.12 ± 0.03 (HH), (40.05 ± 0.15) (Ascorbic acid) μg/mL against ABTS respectively. Statistical analysis indicated that HH caused a competitive type of inhibition of α-amylase (Vmax remained constant and Km increases from 10.65 to 84.37%) while Glucophage caused the un-competitive type of inhibition i-e both Km and Vmax decreased from 40.49 to 69.15% and 38.86 to 69.61% respectively. The Ki, (inhibition constant); KI, (dissociation constant), Km, (Michaelis-Menten constant), and IC50 were found to be 62, 364, 68.1, and 38.08 ± 0.22 for HH and 12, 101.05, 195, 34.01 ± 0.21 for Glucophage. Similarly, HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while Vmax decreases from 60.5% to 74.1%. The calculated Ki, KI, Km, and IC50 were found to be 32, 36.2, 0.05, and 18.117 ± 0.018.

Conclusion: From the current results, it is concluded that HH extract contains bioactive compounds, and could be a good alternative to controlling oxidants, Alzheimer's and Type-II diabetic diseases.

目的研究杂交马钱子(HH)作为自由基清除剂以及α-淀粉酶(α-amylase)和乙酰胆碱酯酶(AChE)两种酶抑制剂的作用:本研究对 HH 植物进行了初步的植物化学筛选分析,然后通过标准程序测试其抗氧化、抗α-淀粉酶和抗 AChE 的功效:结果:植物化学分析显示存在不同的化合物,但不含香豆素和醌类化合物。植物提取物的总酚、类黄酮和单宁含量分别为(78.52 ± 0.69)毫克 GAE/克、(2.01 ± 0.04)毫克 RUE/克和(58.12 ± 0.23)毫克 TAE/克。HH 提取物中含有 28.02% ± 0.02% 生物碱和 2.02% ± 0.05% 皂甙。HH 提取物具有抗氧化特性,对还原铁的 IC50(50% 抑制率)为(151.01 ± 0.13)(HH)、(79.01 ± 0.04)(抗坏血酸)、(91.48 ± 0.13)(HH)、(48.02 ± 0.11)(抗坏血酸)、(156.02 ± 0.31)(HH)、(52.38 ± 0.21)(抗坏血酸)抗 DPPH,136.01 ± 0.21(HH)、52.02± 0.31(抗坏血酸)抗 H2O2,154.12 ± 0.03(HH)、(40.05 ± 0.15)(抗坏血酸)μg/mL 抗 ABTS。统计分析表明,HH 对 α 淀粉酶的抑制作用属于竞争型(Vmax 保持不变,Km 从 10.65% 上升到 84.37%),而 Glucophage 则属于非竞争型抑制作用,即 Km 和 Vmax 分别从 40.49% 下降到 69.15% 和 38.86% 下降到 69.61%。发现 HH 的 Ki(抑制常数)、KI(解离常数)、Km(迈克尔斯-门顿常数)和 IC50 分别为 62、364、68.1 和 38.08 ± 0.22,而 Glucophage 的 Ki、KI、Km 和 IC50 分别为 12、101.05、195 和 34.01 ± 0.21。同样,HH 会对 AChE 产生非竞争性抑制,即 Km 保持不变,而 Vmax 则从 60.5% 下降到 74.1%。计算得出的 Ki、KI、Km 和 IC50 分别为 32、36.2、0.05 和 18.117 ± 0.018:从目前的结果来看,HH 提取物含有生物活性化合物,可作为控制氧化剂、老年痴呆症和 II 型糖尿病的良好替代品。
{"title":"Phytochemical analysis, antioxidant, acetylcholinesterase, and α-amylase inhibitors from extract.","authors":"Naila Sher, Mushtaq Ahmed, Nadia Mushtaq","doi":"10.19852/j.cnki.jtcm.2024.03.001","DOIUrl":"10.19852/j.cnki.jtcm.2024.03.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of <i>Hippeastrum hybridum</i> (HH) as a free radical scavenger, and an inhibitor of the two enzymes i-e Alpha-amylase (α-amylase) and acetylcholinesterase (AChE).</p><p><strong>Methods: </strong>In this study, HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant, anti-α-amylase, and anti-AChE efficiency <i>via</i> standard procedures.</p><p><strong>Results: </strong>Phytochemical analysis shows the existence of different compounds; while Coumarins and quinones were absent. The total phenolic, flavonoid, and tannins content were found to be (78.52 ± 0.69) mg GAE/g, (2.01 ± 0.04) mg RUE/g, and (58.12 ± 0.23) mg TAE/g of plant extract respectively. 28.02% ± 0.02% alkaloid and 2.02% ± 0.05% saponins were present in the HH extract. The HH extract showed the anti-oxidant property with IC50 (50% inhibition) of (151.01 ± 0.13) (HH), (79.01 ± 0.04) (Ascorbic acid) for ferric reducing, (91.48 ± 0.13) (HH), (48.02 ± 0.11) (Ascorbic acid) against Ammonium molybdenum, (156.02 ± 0.31) (HH), (52.38 ± 0.21) (Ascorbic acid) against DPPH, 136.01 ± 0.21 (HH), 52.02± 0.31 (Ascorbic acid) against H<sub>2</sub>O<sub>2</sub>, and 154.12 ± 0.03 (HH), (40.05 ± 0.15) (Ascorbic acid) μg/mL against ABTS respectively. Statistical analysis indicated that HH caused a competitive type of inhibition of α-amylase (<i>V<sub>max</sub></i> remained constant and Km increases from 10.65 to 84.37%) while Glucophage caused the un-competitive type of inhibition i-e both Km and <i>V<sub>max</sub></i> decreased from 40.49 to 69.15% and 38.86 to 69.61% respectively. The <i>K<sub>i</sub></i>, (inhibition constant); <i>K<sub>I</sub></i>, (dissociation constant), <i>K<sub>m</sub></i>, (Michaelis-Menten constant), and IC50 were found to be 62, 364, 68.1, and 38.08 ± 0.22 for HH and 12, 101.05, 195, 34.01 ± 0.21 for Glucophage. Similarly, HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while <i>V<sub>max</sub></i> decreases from 60.5% to 74.1%. The calculated <i>K<sub>i</sub></i>, <i>K<sub>I</sub></i>, <i>K<sub>m</sub></i>, and IC50 were found to be 32, 36.2, 0.05, and 18.117 ± 0.018.</p><p><strong>Conclusion: </strong>From the current results, it is concluded that HH extract contains bioactive compounds, and could be a good alternative to controlling oxidants, Alzheimer's and Type-II diabetic diseases.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"496-504"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Huoxue Jiedu Huayu recipe inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats. 藿雪解毒汤能抑制巨噬细胞分泌的血管内皮生长因子-a对血管生成的影响,减轻单侧输尿管梗阻大鼠对侧肾脏的肾纤维化。
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.20240423.005
Gao Xiaomeng, Qiang Panpan, Chang Jingyue, Fan Lili, Yang Fan, X U Qingyou

OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe (, HJHR) regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction (UUO) rats and the mechanism by which it reduces of renal fibrosis.

Methods: Male Wistar rats were randomly divided into 4 groups: the sham group, UUO group (180 d of left ureter ligation), UUO plus eplerenone (EPL) group, and UUO plus HJHR group. After 180 d of oral drug administration, blood and contralateral kidneys were collected for analysis. Angiogenesis- and fibrosis-related indexes were detected.

Results: HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels of α-smooth muscle actin (α-SMA), vimentin and collagen I. Moreover, these treatments could reduce the expression of vascular endothelial growth factor-A (VEGFA) by inhibiting the infiltration of macrophages. Furthermore, HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production, which inhibited angiogenesis. Finally, the coexpressions of CD34, CD105 and α-SMA were decreased in the HJHR and EPL groups, indicating that endothelial-to-mesenchymal transition was inhibited.

Conclusions: These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFA-induced angiogenesis, encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.

目的:阐明藿香正气水调节单侧输尿管梗阻(UUO)大鼠对侧肾血管生成的机制及其减轻肾脏纤维化的作用机制:雄性Wistar大鼠随机分为4组:假组、UUO组(左输尿管结扎180 d)、UUO加依普利酮(EPL)组和UUO加HJHR组。口服药物 180 天后,收集血液和对侧肾脏进行分析。检测血管生成和纤维化相关指标:结果:HJHR和EPL改善了对侧肾脏的结构损伤和肾间质纤维化,降低了α-平滑肌肌动蛋白(α-SMA)、波形蛋白和胶原蛋白I的蛋白表达水平。此外,HJHR 和 EPL 还能通过下调血管内皮生长因子-A 的产生来显著降低 CD34 和 CD105 的表达,从而抑制血管生成。最后,HJHR 组和 EPL 组 CD34、CD105 和 α-SMA 的共表达减少,表明内皮细胞向间质转化受到抑制:这些研究结果证实,HJHR 可通过抑制 VEGFA 诱导的血管生成缓解对侧肾脏纤维化,鼓励使用 HJHR 治疗肾间质纤维化,并为 CKD 患者的临床治疗提供理论依据。
{"title":"Huoxue Jiedu Huayu recipe inhibits macrophage-secreted vascular endothelial growth factor-a on angiogenesis and alleviates renal fibrosis in the contralateral kidneys of unilateral ureteral obstruction rats.","authors":"Gao Xiaomeng, Qiang Panpan, Chang Jingyue, Fan Lili, Yang Fan, X U Qingyou","doi":"10.19852/j.cnki.jtcm.20240423.005","DOIUrl":"10.19852/j.cnki.jtcm.20240423.005","url":null,"abstract":"<p><p>OBJECTIVE:To elucidate the mechanism by which Huoxue Jiedu Huayu recipe (, HJHR) regulates angiogenesis in the contralateral kidney of unilateral ureteral obstruction (UUO) rats and the mechanism by which it reduces of renal fibrosis.</p><p><strong>Methods: </strong>Male Wistar rats were randomly divided into 4 groups: the sham group, UUO group (180 d of left ureter ligation), UUO plus eplerenone (EPL) group, and UUO plus HJHR group. After 180 d of oral drug administration, blood and contralateral kidneys were collected for analysis. Angiogenesis- and fibrosis-related indexes were detected.</p><p><strong>Results: </strong>HJHR and EPL improved structural damage and renal interstitial fibrosis in the contralateral kidney and reduced the protein expression levels of α-smooth muscle actin (α-SMA), vimentin and collagen I. Moreover, these treatments could reduce the expression of vascular endothelial growth factor-A (VEGFA) by inhibiting the infiltration of macrophages. Furthermore, HJHR and EPL significantly reduced the expression of CD34 and CD105 by downregulating VEGFA production, which inhibited angiogenesis. Finally, the coexpressions of CD34, CD105 and α-SMA were decreased in the HJHR and EPL groups, indicating that endothelial-to-mesenchymal transition was inhibited.</p><p><strong>Conclusions: </strong>These findings confirm that HJHR alleviates contralateral renal fibrosis by inhibiting VEGFA-induced angiogenesis, encourage the use of HJHR against renal interstitial fibrosis and provide a theoretical basis for the clinical management of patients with CKD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"458-467"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identifying the geographical origin and processing technology of Moyao () on the basis of near-infrared spectroscopy combined with chemometrics. 根据近红外光谱与化学计量学相结合的方法,确定莫瑶()的地理产地和加工技术。
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.20240308.002
X U Ningning, Yan Ganming, X U Fengjie, Deng Linfeng, Qiao Xinjiang, L U Changzheng, Cheng Shaomin

Objective: To evaluate the quality of Moyao (Myrrh) in the identification of the geographical origin and processing of the products.

Methods: Raw Moyao (Myrrh) and two kinds of Moyao (Myrrh) processed with vinegar from three countries were identified using near-infrared (NIR) spectroscopy combined with chemometric techniques. Principal component analysis (PCA) was used to reduce the dimensionality of the data and visualize the clustering of samples from different categories. A classical chemometric algorithm (PLS-DA) and two machine learning algorithms [K-nearest neighbor (KNN) and support vector machine] were used to conduct a classification analysis of the near-infrared spectra of the Moyao (Myrrh) samples, and their discriminative performance was evaluated.

Results: Based on the accuracy, precision, recall rate, and F1 value in each model, the results showed that the classical chemometric algorithm and the machine learning algorithm obtained positive results. In all of the chemometric analyses, the NIR spectrum of Moyao (Myrrh) preprocessed by standard normal variation or Multivariate scattering correction combined with KNN achieved the highest accuracy in identifying the geographical origins, and the accuracy of identifying the processing technology established by the KNN method after first-order derivative pretreatment was the best. The best accuracy of geographical origin discrimination and processing technology discrimination were 0.9853 and 0.9706 respectively.

Conclusions: NIR spectroscopy combined with chemometric technology can be an important tool for tracking the origin and processing technology of Moyao (Myrrh) and can also provide a reference for evaluations of its quality and the clinical use.

目的:评估没药的质量:在确定产品的地理来源和加工过程中评估没药的质量:使用近红外光谱(NIR)和化学计量学技术对来自三个国家的未加工的没药和用醋加工的两种没药进行鉴定。主成分分析(PCA)用于降低数据的维度,并使不同类别样品的聚类可视化。采用经典的化学计量学算法(PLS-DA)和两种机器学习算法(K-近邻(KNN)和支持向量机)对没药样品的近红外光谱进行了分类分析,并对其鉴别性能进行了评估:根据各模型的准确度、精确度、召回率和 F1 值,结果表明经典化学计量学算法和机器学习算法都取得了积极的成果。在所有化学计量分析中,经标准正态变异或多元散射校正结合 KNN 预处理的没药近红外光谱的地理产地鉴别准确率最高,KNN 方法在一阶导数预处理后建立的加工工艺鉴别准确率最好。地理产地判别和加工技术判别的最佳准确率分别为 0.9853 和 0.9706:近红外光谱与化学计量学技术相结合可作为追踪没药产地和加工工艺的重要工具,也可为没药质量评价和临床使用提供参考。
{"title":"Identifying the geographical origin and processing technology of Moyao () on the basis of near-infrared spectroscopy combined with chemometrics.","authors":"X U Ningning, Yan Ganming, X U Fengjie, Deng Linfeng, Qiao Xinjiang, L U Changzheng, Cheng Shaomin","doi":"10.19852/j.cnki.jtcm.20240308.002","DOIUrl":"10.19852/j.cnki.jtcm.20240308.002","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the quality of Moyao (<i>Myrrh</i>) in the identification of the geographical origin and processing of the products.</p><p><strong>Methods: </strong>Raw Moyao (<i>Myrrh</i>) and two kinds of Moyao (<i>Myrrh</i>) processed with vinegar from three countries were identified using near-infrared (NIR) spectroscopy combined with chemometric techniques. Principal component analysis (PCA) was used to reduce the dimensionality of the data and visualize the clustering of samples from different categories. A classical chemometric algorithm (PLS-DA) and two machine learning algorithms [K-nearest neighbor (KNN) and support vector machine] were used to conduct a classification analysis of the near-infrared spectra of the Moyao (<i>Myrrh</i>) samples, and their discriminative performance was evaluated.</p><p><strong>Results: </strong>Based on the accuracy, precision, recall rate, and F1 value in each model, the results showed that the classical chemometric algorithm and the machine learning algorithm obtained positive results. In all of the chemometric analyses, the NIR spectrum of Moyao (<i>Myrrh</i>) preprocessed by standard normal variation or Multivariate scattering correction combined with KNN achieved the highest accuracy in identifying the geographical origins, and the accuracy of identifying the processing technology established by the KNN method after first-order derivative pretreatment was the best. The best accuracy of geographical origin discrimination and processing technology discrimination were 0.9853 and 0.9706 respectively.</p><p><strong>Conclusions: </strong>NIR spectroscopy combined with chemometric technology can be an important tool for tracking the origin and processing technology of Moyao (<i>Myrrh</i>) and can also provide a reference for evaluations of its quality and the clinical use.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"505-514"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comprehensive review on pharmacognosy, phytochemistry and pharmacological activities of 8 potent species of southeast Asia. 对东南亚 8 种药用植物的药理、植物化学和药理活性的全面综述。
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.2024.03.002
Shikha Agrawal, Adarsh Kumar, Ankit Kumar Singh, Harshwardhan Singh, Suresh Thareja, Pradeep Kumar

Genus Prunus comprising around 430 species is a vast important genus of family Rosaceae, subfamily amygdalaoidae. Among all 430 species, around 19 important species are commonly found in Indian sub-continent due to their broad nutritional and economic importance. Some most common species of genus Prunus are Prunus amygdalus, Prunus persica, Prunus armeniaca, Prunus avium, Prunus cerasus, Prunus cerasoides, Prunus domestica, Prunus mahaleb, etc. A newly introduced species of Prunus i.e Prunus sunhangii is recently discovered which is morphologically very similar to Prunus cerasoides. Plants of Prunus species are short to medium-sized deciduous trees mainly found in the northern hemisphere. In India and its subcontinent, it extends from the Himalayas to Sikkim, Meghalaya, Bhutan, Myanmar etc. Different Prunus species have been extensively studied for their morphological, microscopic, pharmacological and phytoconstituents characteristics. Total phenolic content of Prunus species explains the presence of phenols in high quantity and pharmacological activity due to phenols. Phytochemical screening of species of genus Prunus shows the presence of wide phytoconstituents which contributes in their pharmacological significance and reveals the therapeutic potential and traditional medicinal significance of this genus. Genus Prunus showed a potent antioxidant activity analyzed by 1,1-diphenyl-2-picryl-hydrazyl radical assay. Plant species belonging to the genus Prunus is widely used traditionally for the treatment of various disorders. Some specific Prunus species possess potent anticancer, anti-inflammatory, hypoglycemic etc. activity which makes the genus more interesting for further research and findings. This review is an attempt to summarize the comprehensive study of Prunus.

樱桃属(Prunus)约有 430 个品种,是蔷薇科(Rosaceae)山杏亚科(Amygdalaoidae)的一个重要属种。在所有 430 个物种中,约有 19 个重要物种常见于印度次大陆,因为它们具有广泛的营养和经济价值。樱桃属中最常见的一些品种有 Prunus amygdalus、Prunus persica、Prunus armeniaca、Prunus avium、Prunus cerasus、Prunus cerasoides、Prunus domestica、Prunus mahaleb 等。最近发现了一个新引进的 Prunus 品种,即 Prunus sunhangii,它在形态上与 Prunus cerasoides 非常相似。樱桃属植物是中矮落叶乔木,主要分布在北半球。在印度及其次大陆,它从喜马拉雅山延伸到锡金、梅加拉亚、不丹、缅甸等地。人们已经对不同的杨梅品种的形态、显微、药理和植物成分特征进行了广泛的研究。李属植物的总酚含量说明了大量酚类物质的存在以及酚类物质的药理活性。杨梅属植物的植物化学筛选结果表明,杨梅属植物含有多种植物成分,这些成分有助于提高杨梅属植物的药理作用,并揭示了杨梅属植物的治疗潜力和传统药用价值。通过 1,1-二苯基-2-苦基-肼自由基试验分析,杨梅属植物显示出强大的抗氧化活性。梅属植物在传统上被广泛用于治疗各种疾病。一些特定的梅属植物具有很强的抗癌、抗炎、降血糖等活性,这使得该属植物更值得进一步研究和发现。本综述试图总结对李属植物的全面研究。
{"title":"A comprehensive review on pharmacognosy, phytochemistry and pharmacological activities of 8 potent species of southeast Asia.","authors":"Shikha Agrawal, Adarsh Kumar, Ankit Kumar Singh, Harshwardhan Singh, Suresh Thareja, Pradeep Kumar","doi":"10.19852/j.cnki.jtcm.2024.03.002","DOIUrl":"10.19852/j.cnki.jtcm.2024.03.002","url":null,"abstract":"<p><p>Genus <i>Prunus</i> comprising around 430 species is a vast important genus of family Rosaceae, subfamily amygdalaoidae. Among all 430 species, around 19 important species are commonly found in Indian sub-continent due to their broad nutritional and economic importance. Some most common species of genus <i>Prunus</i> are <i>Prunus amygdalus, Prunus persica, Prunus armeniaca, Prunus avium, Prunus cerasus, Prunus cerasoides, Prunus domestica, Prunus mahaleb</i>, etc. A newly introduced species of <i>Prunus i.e Prunus sunhangii</i> is recently discovered which is morphologically very similar to <i>Prunus cerasoides</i>. Plants of <i>Prunus</i> species are short to medium-sized deciduous trees mainly found in the northern hemisphere. In India and its subcontinent, it extends from the Himalayas to Sikkim, Meghalaya, Bhutan, Myanmar <i>etc</i>. Different <i>Prunus</i> species have been extensively studied for their morphological, microscopic, pharmacological and phytoconstituents characteristics. Total phenolic content of <i>Prunus</i> species explains the presence of phenols in high quantity and pharmacological activity due to phenols. Phytochemical screening of species of genus <i>Prunus</i> shows the presence of wide phytoconstituents which contributes in their pharmacological significance and reveals the therapeutic potential and traditional medicinal significance of this genus. Genus <i>Prunus</i> showed a potent antioxidant activity analyzed by 1,1-diphenyl-2-picryl-hydrazyl radical assay. Plant species belonging to the genus <i>Prunus</i> is widely used traditionally for the treatment of various disorders. Some specific <i>Prunus</i> species possess potent anticancer, anti-inflammatory, hypoglycemic etc. activity which makes the genus more interesting for further research and findings. This review is an attempt to summarize the comprehensive study of Prunus.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"620-628"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomics analysis of coronary atherosclerotic heart disease with different Traditional Chinese Medicine syndrome types before and after percutaneous coronary intervention. 经皮冠状动脉介入治疗前后不同中医证型冠状动脉粥样硬化性心脏病的蛋白质组学分析
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.20240408.001
Wang Zhibo, L I Ying, Wang Daoping, M A Bo, Miao Lan, Ren Junguo, Liu Jinghua, Liu Jianxun

Objective: To investigate the underlying protein molecular mechanisms of "Qi stagnation and blood stasis syndrome" (QS) and "Qi deficiency and blood stasis syndrome" (QD), as two subtypes of coronary artery disease (CAD) in Traditional Chinese Medicine (TCM), following percutaneous coronary intervention (PCI).

Methods: In this study, a total of 227 CAD patients with QS and 211 CAD patients with QD were enrolled; all participants underwent PCI. Label-free quantification proteomics were employed to analyze the changes in serum in two subtypes of CAD patients before and 6 months after PCI, aiming to elucidate the intervention mechanism of PCI in treating CAD characterized by two different TCM syndromes.

Results: Biochemical analysis revealed significant changes in tumor necrosis factor-α, high density lipoprotein cholesterol, blood stasis clinical symptoms observation, and Gensini levels in both patient groups post-PCI; Proteomic analysis identified 79 and 95 differentially expressed proteins in the QS and QD patient groups, respectively, compared to their control groups. complement C8 alpha chain, complement factor H, apolipoprotein H, apolipoprotein B, plasminogen, carbonic anhydrase 2, and complement factor I were altered in both comparison groups. Furthermore, enrichment analysis demonstrated that cell adhesion and connectivity-related processes underwent changes in QS patients post-PCI, whereas lipid metabolism-related pathways, including the peroxisome proliferator-activated receptor signaling pathway and extracellular matrix receptor interaction, underwent changes in the QD group. The protein-protein interaction network analysis further enriched 52 node proteins, including apolipoprotein B, lipoprotein (a), complement C5, apolipoprotein A4, complement C8 alpha chain, complement C8 beta chain, complement C8 gamma chain, apolipoprotein H, apolipoprotein A-Ⅱ, albumin, complement C4-B, apolipoprotein C3, among others. The functional network of these proteins is posited to contribute to the pathophysiology of CAD characterized by TCM syndromes.

Conclusion: The current quantitative proteomic study has preliminarily identified biomarkers of CAD in different TCM subtypes treated with PCI, potentially laying the groundwork for understanding the protein profiles associated with the treatment of various TCM subtypes of CAD.

目的研究经皮冠状动脉介入治疗(PCI)后,中医 "气滞血瘀证"(QS)和 "气虚血瘀证"(QD)这两种冠心病亚型的蛋白分子机制:本研究共纳入了 227 例 QS 冠心病患者和 211 例 QD 冠心病患者,所有患者均接受了经皮冠状动脉介入治疗。采用无标记定量蛋白质组学分析两种亚型CAD患者在PCI术前和术后6个月的血清变化,旨在阐明PCI治疗两种不同中医综合征特征的CAD的干预机制:生化分析显示,PCI术后两组患者的肿瘤坏死因子-α、高密度脂蛋白胆固醇、血瘀证临床症状观察指标和Gensini水平均有明显变化;蛋白质组学分析发现,与对照组相比,QS组和QD组分别有79个和95个差异表达蛋白。补体 C8 α 链、补体因子 H、载脂蛋白 H、载脂蛋白 B、纤溶酶原、碳酸酐酶 2 和补体因子 I 在两组对比中均有改变。此外,富集分析表明,PCI 术后 QS 患者的细胞粘附和连接相关过程发生了变化,而 QD 组的脂质代谢相关通路(包括过氧化物酶体增殖激活受体信号通路和细胞外基质受体相互作用)发生了变化。蛋白质-蛋白质相互作用网络分析进一步富集了52个节点蛋白,包括脂蛋白B、脂蛋白(a)、补体C5、脂蛋白A4、补体C8α链、补体C8β链、补体C8γ链、脂蛋白H、脂蛋白A-Ⅱ、白蛋白、补体C4-B、脂蛋白C3等。这些蛋白质的功能网络被认为有助于以中医综合征为特征的 CAD 的病理生理学:目前的定量蛋白质组学研究初步确定了接受 PCI 治疗的不同中医亚型的 CAD 生物标志物,为了解与治疗各种中医亚型 CAD 相关的蛋白质谱奠定了基础。
{"title":"Proteomics analysis of coronary atherosclerotic heart disease with different Traditional Chinese Medicine syndrome types before and after percutaneous coronary intervention.","authors":"Wang Zhibo, L I Ying, Wang Daoping, M A Bo, Miao Lan, Ren Junguo, Liu Jinghua, Liu Jianxun","doi":"10.19852/j.cnki.jtcm.20240408.001","DOIUrl":"10.19852/j.cnki.jtcm.20240408.001","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the underlying protein molecular mechanisms of \"<i>Qi</i> stagnation and blood stasis syndrome\" (QS) and \"<i>Qi</i> deficiency and blood stasis syndrome\" (QD), as two subtypes of coronary artery disease (CAD) in Traditional Chinese Medicine (TCM), following percutaneous coronary intervention (PCI).</p><p><strong>Methods: </strong>In this study, a total of 227 CAD patients with QS and 211 CAD patients with QD were enrolled; all participants underwent PCI. Label-free quantification proteomics were employed to analyze the changes in serum in two subtypes of CAD patients before and 6 months after PCI, aiming to elucidate the intervention mechanism of PCI in treating CAD characterized by two different TCM syndromes.</p><p><strong>Results: </strong>Biochemical analysis revealed significant changes in tumor necrosis factor-α, high density lipoprotein cholesterol, blood stasis clinical symptoms observation, and Gensini levels in both patient groups post-PCI; Proteomic analysis identified 79 and 95 differentially expressed proteins in the QS and QD patient groups, respectively, compared to their control groups. complement C8 alpha chain, complement factor H, apolipoprotein H, apolipoprotein B, plasminogen, carbonic anhydrase 2, and complement factor I were altered in both comparison groups. Furthermore, enrichment analysis demonstrated that cell adhesion and connectivity-related processes underwent changes in QS patients post-PCI, whereas lipid metabolism-related pathways, including the peroxisome proliferator-activated receptor signaling pathway and extracellular matrix receptor interaction, underwent changes in the QD group. The protein-protein interaction network analysis further enriched 52 node proteins, including apolipoprotein B, lipoprotein (a), complement C5, apolipoprotein A4, complement C8 alpha chain, complement C8 beta chain, complement C8 gamma chain, apolipoprotein H, apolipoprotein A-Ⅱ, albumin, complement C4-B, apolipoprotein C3, among others. The functional network of these proteins is posited to contribute to the pathophysiology of CAD characterized by TCM syndromes.</p><p><strong>Conclusion: </strong>The current quantitative proteomic study has preliminarily identified biomarkers of CAD in different TCM subtypes treated with PCI, potentially laying the groundwork for understanding the protein profiles associated with the treatment of various TCM subtypes of CAD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"44 3","pages":"554-563"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11077157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical efficacy and gene chip expression analysis of Shenzhu Guanxin recipe granules in patients with intermediate coronary lesions. 神珠贯心配方颗粒对冠状动脉中级病变患者的临床疗效及基因芯片表达分析
Pub Date : 2024-06-01 DOI: 10.19852/j.cnki.jtcm.20240308.004
Jin Xiao, W U Bingxin, Lin Miaoyang, Zhong Biying, Lin Luoqi, X U Danping

Objective: To evaluate the clinical efficacy and safety of Shenzhu Guanxin recipe granules (, SGR) in treating patients with intermediate coronary lesions (ICL), and to investigate the potential mechanism though a transcriptome sequencing approach.

Methods: ICL patients with Qi deficiency and phlegm stasis were adopted and randomly assigned to a case group or a control by random number generator in a 1:1 randomization ratio to evaluate the clinical efficacy.

Results: There was no significant difference between the two groups in coronary computed tomography angiography related indexes in the two groups before and after intervention. Through the gene chip expression analysis, it is finally concluded that there are 355 differential mRNAs (190 up-regulated genes and 165 down regulated genes) when compared the SGR group and placebo group. Through protein-protein interaction network analysis of differentially expressed genes, 10 hub genes were finally obtained: CACNA2D2, CACNA2D3, DNAJC6, FGF12, SGSM2, CACNA1G, LRP6, KIF25, OXTR, UPB1.

Conclusions: SGR combined with Western Medicine can be safely used to treat ICL patients with Qi deficiency and phlegm stasis. The possible mechanism of action and relevant gene loci and pathway were proposed.

目的方法:采用气虚痰瘀型ICL患者为研究对象,通过随机发生器按1:1的随机比例将其随机分为病例组和对照组,评价其临床疗效和安全性:方法:采用随机数字生成器将气虚痰瘀型冠心病中期病变患者按1:1的随机比例随机分为病例组和对照组,评价其临床疗效:结果:干预前后两组冠状动脉计算机断层扫描血管造影相关指标无明显差异。通过基因芯片表达分析,最终得出结论:SGR组与安慰剂组相比,有355个差异mRNA(190个上调基因和165个下调基因)。通过对差异表达基因的蛋白-蛋白相互作用网络分析,最终得到了 10 个中心基因:CACNA2D2、CACNA2D3、DNAJC6、FGF12、SGSM2、CACNA1G、LRP6、KIF25、OXTR、UPB1:结论:SGR联合西药治疗气虚痰瘀型ICL患者是安全的。结论:SGR联合西药治疗气虚痰瘀型ICL患者疗效确切,并提出了可能的作用机制及相关基因位点和途径。
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引用次数: 0
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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
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