Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.013
Zhang Lina, Lin Xiu, Zhao Xin, L I Wenjuan, Zhao Ye
Objective: To evaluate the effect of Fuzi Lizhong decoction on intestinal flora, serum inflammatory factors, and hypoxia inducible factor-1α (HIF-1α) in patients with colorectal cancer associated with spleen and kidney Yang deficiency.
Methods: A total of 100 patients diagnosed with advanced colorectal cancer were randomly divided into two groups: a control group (CON, 50) and a Traditional Chinese Medicine (TCM) group (n = 50). The control group received treatment with the Capecitabine + Oxaliplatin (CAPEOX) regimen, while the TCM group received the same regimen along with Fuzi Lizhong decoction for six weeks. Changes in intestinal flora were assessed before and after six weeks in both groups. Serum markers, including HIF-1α, vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were measured using enzyme-linked immunosorbent assay. Adverse reactions, clinical efficacy, and TCM syndrome efficacy were also monitored.
Results: After six weeks, the levels of Lactobacillus and Bifidobacterium were significantly higher, while the levels of Enterobacter and Enterococcus were significantly lower in the TCM group compared to the control group (P < 0.05). Serum levels of HIF-1α, VEGF, IL-6, and TNF-α were also significantly reduced in the TCM group compared to the control group (P < 0.05). Additionally, the incidence of adverse reactions was lower, and the clinical efficacy was higher in the TCM group compared to the control group (P < 0.05).
Conclusion: Fuzi Lizhong decoction effectively improves intestinal microbiota composition, reduces inflammatory factors and HIF-1α expression, alleviates chemotherapy-related adverse reactions, enhances clinical efficacy, and may inhibit tumor growth in patients with colorectal cancer.
{"title":"Study on the mechanism of Fuzi Lizhong decoction in the treatment of colorectal cancer of spleen kidney deficiency from the perspective of intestinal flora and hypoxia inducible factor-1α signalling pathway.","authors":"Zhang Lina, Lin Xiu, Zhao Xin, L I Wenjuan, Zhao Ye","doi":"10.19852/j.cnki.jtcm.2025.04.013","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.013","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of Fuzi Lizhong decoction on intestinal flora, serum inflammatory factors, and hypoxia inducible factor-1α (HIF-1α) in patients with colorectal cancer associated with spleen and kidney <i>Yang</i> deficiency.</p><p><strong>Methods: </strong>A total of 100 patients diagnosed with advanced colorectal cancer were randomly divided into two groups: a control group (CON, 50) and a Traditional Chinese Medicine (TCM) group (<i>n =</i> 50). The control group received treatment with the Capecitabine + Oxaliplatin (CAPEOX) regimen, while the TCM group received the same regimen along with Fuzi Lizhong decoction for six weeks. Changes in intestinal flora were assessed before and after six weeks in both groups. Serum markers, including HIF-1α, vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were measured using enzyme-linked immunosorbent assay. Adverse reactions, clinical efficacy, and TCM syndrome efficacy were also monitored.</p><p><strong>Results: </strong>After six weeks, the levels of Lactobacillus and Bifidobacterium were significantly higher, while the levels of Enterobacter and Enterococcus were significantly lower in the TCM group compared to the control group (<i>P <</i> 0.05). Serum levels of HIF-1α, VEGF, IL-6, and TNF-α were also significantly reduced in the TCM group compared to the control group (<i>P <</i> 0.05). Additionally, the incidence of adverse reactions was lower, and the clinical efficacy was higher in the TCM group compared to the control group (<i>P <</i> 0.05).</p><p><strong>Conclusion: </strong>Fuzi Lizhong decoction effectively improves intestinal microbiota composition, reduces inflammatory factors and HIF-1α expression, alleviates chemotherapy-related adverse reactions, enhances clinical efficacy, and may inhibit tumor growth in patients with colorectal cancer.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"845-851"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.006
Wang Raoqiong, Hao Linyao, L U Ye, Wang Lingxue, L I Jianrong, Peng Yan, Tang Hongmei, L I Shuangyang, Bai Xue
Objective: To analyze the mechanism of Tongqiao Yizhi decoction (TQYZD, ) in treating vascular dementia (VaD) rats using brain tissue untargeted metabonomics and fecal 16S rRNA gene sequencing.
Methods: The chemical composition of TQYZD was analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry. The effects of TQYZD on VaD rats were evaluated by water maze test, hematoxylin-eosin staining, enzyme-linked immunosorbent assay and Western blot. Untargeted metabolomics and 16S rRNA sequencing were utilized to explore the relationship between metabolic profiles and the structure and function of intestinal flora. Fecal microbiota transplantation (FMT) was used to validate the role of gut microbiota in VaD. Spearman correlation analysis of differential metabolites and gut microbiota was performed.
Results: The results showed that TQYZD improved cognitive function and neuronal damage in VaD rats, and reduced inflammatory response and repaired the intestinal barrier. In addition, TQYZD had the effect of modulating gut microbes in VaD rats, and FMT further confirmed that gut flora plays an important role in TQYZD treatment of VaD. Untargeted metabolomics revealed that VaD could lead to metabolic disorders in brain tissues, and TQYZD significantly altered the metabolites of brain tissues in Middle Cerebral Artery Occlusion rats. The results of spearman correlation analysis showed that there was a significant correlation between intestinal flora and the metabolites of brain tissues.
Conclusion: In this study, we demonstrated that TQYZD can improve metabolic disorders in vascular dementia rats by acting on intestinal flora.
{"title":"Mechanism analysis of Tongqiao Yizhi decoction in treating vascular dementia rats by brain tissue untargeted metabonomics and fecal 16S rRNA gene sequencing.","authors":"Wang Raoqiong, Hao Linyao, L U Ye, Wang Lingxue, L I Jianrong, Peng Yan, Tang Hongmei, L I Shuangyang, Bai Xue","doi":"10.19852/j.cnki.jtcm.2025.04.006","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.006","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the mechanism of Tongqiao Yizhi decoction (TQYZD, ) in treating vascular dementia (VaD) rats using brain tissue untargeted metabonomics and fecal 16S rRNA gene sequencing.</p><p><strong>Methods: </strong>The chemical composition of TQYZD was analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry. The effects of TQYZD on VaD rats were evaluated by water maze test, hematoxylin-eosin staining, enzyme-linked immunosorbent assay and Western blot. Untargeted metabolomics and 16S rRNA sequencing were utilized to explore the relationship between metabolic profiles and the structure and function of intestinal flora. Fecal microbiota transplantation (FMT) was used to validate the role of gut microbiota in VaD. Spearman correlation analysis of differential metabolites and gut microbiota was performed.</p><p><strong>Results: </strong>The results showed that TQYZD improved cognitive function and neuronal damage in VaD rats, and reduced inflammatory response and repaired the intestinal barrier. In addition, TQYZD had the effect of modulating gut microbes in VaD rats, and FMT further confirmed that gut flora plays an important role in TQYZD treatment of VaD. Untargeted metabolomics revealed that VaD could lead to metabolic disorders in brain tissues, and TQYZD significantly altered the metabolites of brain tissues in Middle Cerebral Artery Occlusion rats. The results of spearman correlation analysis showed that there was a significant correlation between intestinal flora and the metabolites of brain tissues.</p><p><strong>Conclusion: </strong>In this study, we demonstrated that TQYZD can improve metabolic disorders in vascular dementia rats by acting on intestinal flora.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"759-769"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.018
K E Tianxingjian, Chen Wanjia, Xiang Ling, Deng Yueyi, Wang Yiquan, Liu Wangyi, Xing Yue, L U Zhenzhen, Gao Hongzhi
<p><strong>Objective: </strong>To evaluate the 10-year therapeutic efficacy of Traditional Chinese Medicine (TCM) using the Strengthening Spleen and Draining Dampness therapy in the management of idiopathic membranous nephropathy (IMN).</p><p><strong>Methods: </strong>A single-center, retrospective analysis was conducted on patients diagnosed with IMN who met predefined inclusion and exclusion criteria. Data were collected from the Department of Nephrology at Longhua Hospital, affiliated with Shanghai University of Traditional Chinese Medicine, between January 2007 and December 2011. Clinical parameters including 24-h urinary protein, serum albumin, serum creatinine, and estimated glomerular filtration rate (eGFR, EPI) were assessed at baseline and at 1, 3, 5, and 10 years of follow-up. The efficacy of the Strengthening Spleen and Draining Dampness therapy was analyzed using repeated measures analysis of variance (ANOVA). Kaplan-Meier survival curves and multivariate proportional hazards model (Cox regression models) were employed to identify factors associated with treatment outcomes.</p><p><strong>Results: </strong>A total of 265 patients were included, with a median follow-up duration of 96 months (36, 122). TCM treatment significantly reduced 24-h urinary protein levels (<i>P <</i> 0.001), and increased serum albumin levels (<i>P <</i> 0.001), while serum creatinine remained stable (<i>P =</i> 0.187). Remission rates at 1, 3, 5, and 10 years were 52.81%, 69.71%, 68.39%, and 72.36%, respectively, and the rates of avoiding composite outcome events at the same intervals were 98.27%, 94.29%, 94.19%, and 93.50%. In the subgroup receiving TCM only, remission rates were 56.67%, 84.44%, 76.32%, and 82.86%. For patients treated initially with Western Medicine followed by TCM, the rates were 52.83%, 65.85%, 67.47% and 67.75%. In the cohort of patients who received TCM as their first-line therapy, remission rates were 49.23%, 62.50%, 61.76%, and 69.23%. Multivariate Cox regression analysis revealed that the duration of TCM treatment [hazard ratio (<i>HR</i>) = 0.826, 95% confidence interval (<i>CI</i>) (0.779, 0.876), <i>P <</i> 0.001], presence of hypertension [<i>HR</i> = 1.912, 95% <i>CI</i> (1.181, 3.094), <i>P =</i> 0.008], baseline serum albumin level [<i>HR</i> = 0.930, 95% <i>CI</i> (0.894, 0.969), <i>P <</i> 0.001], and the rate of serum albumin increase within the first year of treatment [<i>HR</i> = 0.930, 95% <i>CI</i> (0.909, 0.957), <i>P <</i> 0.001] were significantly associated with clinical outcomes.</p><p><strong>Conclusion: </strong>The Strengthening Spleen and Draining Dampness therapy demonstrated robust short- and long-term efficacy in treating IMN, with high rates of remission and renal survival over 10 years. Key factors influencing clinical remission included the duration of TCM treatment, baseline serum albumin levels, the presence of hypertension, and the rate of increase in serum albumin within the first year. These findings
目的：评价健脾疏湿法治疗特发性膜性肾病（IMN）的10年疗效。方法：对符合预定纳入和排除标准的诊断为IMN的患者进行单中心回顾性分析。数据收集自上海中医药大学附属龙华医院肾内科，时间为2007年1月至2011年12月。临床参数包括24小时尿蛋白、血清白蛋白、血清肌酐和估计肾小球滤过率（eGFR， EPI）在基线和1年、3年、5年和10年随访时进行评估。采用重复测量方差分析（ANOVA）对健脾疏湿疗法的疗效进行分析。采用Kaplan-Meier生存曲线和多变量比例风险模型（Cox回归模型）确定与治疗结果相关的因素。结果：共纳入265例患者，中位随访时间96个月（36,122）。中药治疗显著降低了24小时尿蛋白水平（P < 0.001），提高了血清白蛋白水平（P < 0.001），而血清肌酐保持稳定（P = 0.187）。1年、3年、5年和10年的缓解率分别为52.81%、69.71%、68.39%和72.36%，相同时间间隔内避免复合结局事件的发生率分别为98.27%、94.29%、94.19%和93.50%。单纯中药治疗组的缓解率分别为56.67%、84.44%、76.32%和82.86%。西医先治后中医的患者分别为52.83%、65.85%、67.47%和67.75%。在以中药为一线治疗的患者队列中，缓解率分别为49.23%、62.50%、61.76%和69.23%。多因素Cox回归分析显示，中药治疗持续时间[风险比(HR) = 0.826, 95%可信区间（CI） （0.779, 0.876）， P 0.001]、是否存在高血压[HR = 1.912, 95% CI (1.181, 3.094), P = 0.008]、基线血清白蛋白水平[HR = 0.930, 95% CI (0.894, 0.969), P 0.001]、治疗一年内血清白蛋白升高率[HR = 0.930, 95% CI (0.909, 0.957), P 0.001]与临床结局有显著相关。结论：健脾疏湿法治疗IMN具有较强的短期和长期疗效，缓解率高，10年以上肾生存率高。影响临床缓解的关键因素包括中医治疗的持续时间、基线血清白蛋白水平、是否存在高血压以及一年内血清白蛋白的升高率。这些发现表明，这种中医方法为IMN提供了一种可行的长期治疗选择。
{"title":"Clinical evaluation of strengthening spleen and draining dampness in the treatment of idiopathic membranous nephropathy: a retrospective 10-year follow-up study.","authors":"K E Tianxingjian, Chen Wanjia, Xiang Ling, Deng Yueyi, Wang Yiquan, Liu Wangyi, Xing Yue, L U Zhenzhen, Gao Hongzhi","doi":"10.19852/j.cnki.jtcm.2025.04.018","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.018","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the 10-year therapeutic efficacy of Traditional Chinese Medicine (TCM) using the Strengthening Spleen and Draining Dampness therapy in the management of idiopathic membranous nephropathy (IMN).</p><p><strong>Methods: </strong>A single-center, retrospective analysis was conducted on patients diagnosed with IMN who met predefined inclusion and exclusion criteria. Data were collected from the Department of Nephrology at Longhua Hospital, affiliated with Shanghai University of Traditional Chinese Medicine, between January 2007 and December 2011. Clinical parameters including 24-h urinary protein, serum albumin, serum creatinine, and estimated glomerular filtration rate (eGFR, EPI) were assessed at baseline and at 1, 3, 5, and 10 years of follow-up. The efficacy of the Strengthening Spleen and Draining Dampness therapy was analyzed using repeated measures analysis of variance (ANOVA). Kaplan-Meier survival curves and multivariate proportional hazards model (Cox regression models) were employed to identify factors associated with treatment outcomes.</p><p><strong>Results: </strong>A total of 265 patients were included, with a median follow-up duration of 96 months (36, 122). TCM treatment significantly reduced 24-h urinary protein levels (<i>P <</i> 0.001), and increased serum albumin levels (<i>P <</i> 0.001), while serum creatinine remained stable (<i>P =</i> 0.187). Remission rates at 1, 3, 5, and 10 years were 52.81%, 69.71%, 68.39%, and 72.36%, respectively, and the rates of avoiding composite outcome events at the same intervals were 98.27%, 94.29%, 94.19%, and 93.50%. In the subgroup receiving TCM only, remission rates were 56.67%, 84.44%, 76.32%, and 82.86%. For patients treated initially with Western Medicine followed by TCM, the rates were 52.83%, 65.85%, 67.47% and 67.75%. In the cohort of patients who received TCM as their first-line therapy, remission rates were 49.23%, 62.50%, 61.76%, and 69.23%. Multivariate Cox regression analysis revealed that the duration of TCM treatment [hazard ratio (<i>HR</i>) = 0.826, 95% confidence interval (<i>CI</i>) (0.779, 0.876), <i>P <</i> 0.001], presence of hypertension [<i>HR</i> = 1.912, 95% <i>CI</i> (1.181, 3.094), <i>P =</i> 0.008], baseline serum albumin level [<i>HR</i> = 0.930, 95% <i>CI</i> (0.894, 0.969), <i>P <</i> 0.001], and the rate of serum albumin increase within the first year of treatment [<i>HR</i> = 0.930, 95% <i>CI</i> (0.909, 0.957), <i>P <</i> 0.001] were significantly associated with clinical outcomes.</p><p><strong>Conclusion: </strong>The Strengthening Spleen and Draining Dampness therapy demonstrated robust short- and long-term efficacy in treating IMN, with high rates of remission and renal survival over 10 years. Key factors influencing clinical remission included the duration of TCM treatment, baseline serum albumin levels, the presence of hypertension, and the rate of increase in serum albumin within the first year. These findings ","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"881-890"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.015
Song Jianfei, Qin Zhengyuan, G U Xinlu, Zhang Yan, L I Xingrui
Objective: To investigate the effects of acupuncture combined with upper limb rehabilitation robot on neural remodeling and functional recovery in post-stroke patients.
Methods: There were 50 stroke patients were randomly divided into an experimental group (acupuncture combined with upper limb rehabilitation robot assisted training) and a control group (upper limb rehabilitation robot assisted training). Various assessments were conducted to compare the effects of the two treatments on neural remodeling and functional recovery. Functional near-infrared spectroscopy technology was used to assess the effects of different treatments on neural plasticity and their impact on upper limb function and activities of daily living.
Results: The experimental group showed significantly higher concentrations of oxygenated hemoglobin and total hemoglobin in specific brain regions compared to the control group (P < 0.05). Additionally, the experimental group had significantly lower concentrations of deoxygenated hemoglobin (P < 0.05). After treatment, both groups showed improvements in various measures, but the experimental group had significantly greater improvements (P < 0.05).
Conclusion: Acupuncture combined with upper limb rehabilitation robot can effectively improve upper limb function and neural remodeling in stroke patients. This study supports the integration of Traditional Chinese and Western Medicine in improving limb dysfunction post-stroke.
{"title":"Efficacy of acupuncture combined with upper limb rehabilitation robot-assisted training for neuroplasticity and functional recovery of patients with stroke: a prospective cohort study based on functional near-infrared spectroscopy technology.","authors":"Song Jianfei, Qin Zhengyuan, G U Xinlu, Zhang Yan, L I Xingrui","doi":"10.19852/j.cnki.jtcm.2025.04.015","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.015","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of acupuncture combined with upper limb rehabilitation robot on neural remodeling and functional recovery in post-stroke patients.</p><p><strong>Methods: </strong>There were 50 stroke patients were randomly divided into an experimental group (acupuncture combined with upper limb rehabilitation robot assisted training) and a control group (upper limb rehabilitation robot assisted training). Various assessments were conducted to compare the effects of the two treatments on neural remodeling and functional recovery. Functional near-infrared spectroscopy technology was used to assess the effects of different treatments on neural plasticity and their impact on upper limb function and activities of daily living.</p><p><strong>Results: </strong>The experimental group showed significantly higher concentrations of oxygenated hemoglobin and total hemoglobin in specific brain regions compared to the control group (<i>P <</i> 0.05). Additionally, the experimental group had significantly lower concentrations of deoxygenated hemoglobin (<i>P <</i> 0.05). After treatment, both groups showed improvements in various measures, but the experimental group had significantly greater improvements (<i>P <</i> 0.05).</p><p><strong>Conclusion: </strong>Acupuncture combined with upper limb rehabilitation robot can effectively improve upper limb function and neural remodeling in stroke patients. This study supports the integration of Traditional Chinese and Western Medicine in improving limb dysfunction post-stroke.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"860-866"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.017
Sun Weiwei, Zhang Jiale, Yang Hanwen, Yan Runze, Wei Shuwu, W U Qiaoru, Cui Zhaoli, Zheng Huijuan, Wang Yaoxian
Objective: To assess the benefits of Qingre Xiaozheng formula (, QRXZF) as an adjunct to standard Western medical management on renal outcomes in patients with diabetic kidney disease (DKD).
Methods: This retrospective study included patients with DKD who received the QRXZF between May 2017 and May 2021. A total of 144 patients with DKD, 24 h urinary total protein (24 h-UTP) ≥ 0.5 g, and estimated glomerular filtration rate (eGFR) ≥ 30 mL/min per 1.73 m2 were divided into the treatment group or the control group based on whether they received QRXZF treatment. The long-term renal outcomes of patients with DKD were analyzed to evaluate the effectiveness of the QRXZF. Differences in overall survival (OS) were assessed using Kaplan-Meier curve analysis. Cox proportional hazards regression analysis was used to determine the independent risk factors for renal endpoints.
Results: The mean follow-up period was (28±15) months. Nine (12.5%) patients in the treatment group and 27 (37.5%) patients in the control group met the renal endpoints. Multivariate Cox regression analysis showed that 24 h-UTP ≥ 3.5 g [hazard ratio (HR) = 4.70, 95% confidence interval (CI) (1.83, 12.05), P = 0.001], combined coronary artery disease [HR = 3.39, 95% CI (1.65, 6.98), P = 0.001], total cholesterol [HR = 1.34, 95% CI (1.05, 1.70), P = 0.019] and low-density lipoprotein [HR = 1.65, 95% CI (1.111, 2.45), P = 0.013] were independent prognostic factors for renal endpoints in patients with DKD. Compared with the treatment group, the risk of renal endpoint events increased 2.68-fold in the control group [HR = 2.68, 95% CI (1.19, 6.02); P = 0.017]. We included 48 patients with 24 h-UTP ≥ 3.5 g in a further stratification analysis of patients with DKD. The independent risk factor for the renal endpoints in patients with 24h-UTP ≥ 3.5 g was smoking history [HR = 5.52, 95% CI (1.131, 26.92), P = 0.035]. Compared with the treatment group, the risk of renal endpoint events increased 3.01-fold in the control group [HR = 3.01, 95% CI (1.05, 8.67); P = 0.041].
Conclusions: The results show that QRXZF treatment improved renal outcomes and reduced proteinuria in patients with DKD. These results indicate that Traditional Chinese Medicine is likely to have a positive therapeutic effect on established and advanced DKD. Further well-designed clinical trials with longer follow-up periods are required.
目的：评价清热消正方辅助西医标准治疗对糖尿病肾病（DKD）患者肾脏预后的影响。方法：本回顾性研究纳入了2017年5月至2021年5月期间接受QRXZF治疗的DKD患者。将144例DKD患者，24 h尿总蛋白(24 h- utp)≥0.5 g，估计肾小球滤过率(eGFR)≥30 mL/min / 1.73 m2，根据是否接受QRXZF治疗分为治疗组和对照组。分析DKD患者的长期肾脏预后，以评估QRXZF的有效性。采用Kaplan-Meier曲线分析评估总生存期（OS）的差异。采用Cox比例风险回归分析确定肾脏终点的独立危险因素。结果：平均随访时间（28±15）个月。治疗组9例（12.5%）和对照组27例（37.5%）患者达到肾脏终点。多因素Cox回归分析显示，24 h-UTP≥3.5 g[危险比(HR) = 4.70, 95%可信区间（CI） （1.83, 12.05）， P = 0.001]、合并冠状动脉疾病[HR = 3.39, 95% CI (1.65, 6.98), P = 0.001]、总胆固醇[HR = 1.34, 95% CI (1.05, 1.70), P = 0.019]和低密度脂蛋白[HR = 1.65, 95% CI (1.111, 2.45), P = 0.013]是DKD患者肾脏终点的独立预后因素。与治疗组相比，对照组发生肾终点事件的风险增加了2.68倍[HR = 2.68, 95% CI (1.19, 6.02)；P = 0.017]。我们纳入48例24 h-UTP≥3.5 g的患者，对DKD患者进行进一步的分层分析。24h-UTP≥3.5 g患者肾脏终点的独立危险因素为吸烟史[HR = 5.52, 95% CI (1.131, 26.92), P = 0.035]。与治疗组相比，对照组发生肾终点事件的风险增加了3.01倍[HR = 3.01, 95% CI (1.05, 8.67)；P = 0.041]。结论：QRXZF治疗改善了DKD患者的肾脏预后，减少了蛋白尿。这些结果表明，中药可能对已建立和晚期DKD有积极的治疗作用。需要进一步设计良好、随访时间较长的临床试验。
{"title":"Traditional Chinese herbal medicine Qingre Xiaozheng formula improves renal outcomes in patients with diabetic kidney disease: a retrospective study.","authors":"Sun Weiwei, Zhang Jiale, Yang Hanwen, Yan Runze, Wei Shuwu, W U Qiaoru, Cui Zhaoli, Zheng Huijuan, Wang Yaoxian","doi":"10.19852/j.cnki.jtcm.2025.04.017","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.017","url":null,"abstract":"<p><strong>Objective: </strong>To assess the benefits of Qingre Xiaozheng formula (, QRXZF) as an adjunct to standard Western medical management on renal outcomes in patients with diabetic kidney disease (DKD).</p><p><strong>Methods: </strong>This retrospective study included patients with DKD who received the QRXZF between May 2017 and May 2021. A total of 144 patients with DKD, 24 h urinary total protein (24 h-UTP) ≥ 0.5 g, and estimated glomerular filtration rate (eGFR) ≥ 30 mL/min per 1.73 m<sup>2</sup> were divided into the treatment group or the control group based on whether they received QRXZF treatment. The long-term renal outcomes of patients with DKD were analyzed to evaluate the effectiveness of the QRXZF. Differences in overall survival (OS) were assessed using Kaplan-Meier curve analysis. Cox proportional hazards regression analysis was used to determine the independent risk factors for renal endpoints.</p><p><strong>Results: </strong>The mean follow-up period was (28±15) months. Nine (12.5%) patients in the treatment group and 27 (37.5%) patients in the control group met the renal endpoints. Multivariate Cox regression analysis showed that 24 h-UTP ≥ 3.5 g [hazard ratio (<i>HR</i>) = 4.70, 95% confidence interval (<i>CI</i>) (1.83, 12.05), <i>P =</i> 0.001], combined coronary artery disease [<i>HR</i> = 3.39, 95% <i>CI</i> (1.65, 6.98), <i>P =</i> 0.001], total cholesterol [<i>HR</i> = 1.34, 95% <i>CI</i> (1.05, 1.70), <i>P =</i> 0.019] and low-density lipoprotein [<i>HR</i> = 1.65, 95% <i>CI</i> (1.111, 2.45), <i>P =</i> 0.013] were independent prognostic factors for renal endpoints in patients with DKD. Compared with the treatment group, the risk of renal endpoint events increased 2.68-fold in the control group [<i>HR</i> = 2.68, 95% <i>CI</i> (1.19, 6.02); <i>P =</i> 0.017]. We included 48 patients with 24 h-UTP ≥ 3.5 g in a further stratification analysis of patients with DKD. The independent risk factor for the renal endpoints in patients with 24h-UTP ≥ 3.5 g was smoking history [<i>HR</i> = 5.52, 95% <i>CI</i> (1.131, 26.92), <i>P =</i> 0.035]. Compared with the treatment group, the risk of renal endpoint events increased 3.01-fold in the control group [<i>HR</i> = 3.01, 95% <i>CI</i> (1.05, 8.67); <i>P =</i> 0.041].</p><p><strong>Conclusions: </strong>The results show that QRXZF treatment improved renal outcomes and reduced proteinuria in patients with DKD. These results indicate that Traditional Chinese Medicine is likely to have a positive therapeutic effect on established and advanced DKD. Further well-designed clinical trials with longer follow-up periods are required.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"873-880"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.008
Feng Dayong, A N Ayue, Feng Yuening, Wang Chunhui, Bai Zhiyong, Wang Qian
Objective: To investigate the mechanism by which An's anorectal fumigation lotion (AAFL) treats inflammatory mixed hemorrhoids.
Methods: Eighty Sprague-Dawley rats, with an equal number of males and females, were randomly assigned to the following four groups: control, model, AAFL, and positive groups. Following hemorrhoid induction, hemorrhoidal tissues were collected from the rats for analysis. Pathological alterations in these tissues were examined via hematoxylin-eosin staining. Immun-ohistochemistry was used to detect inflammatory markers. The ultrastructural pathological changes in these tissues were observed by transmission electron microscopy. Reverse transcription-polymerase chain reaction and Western blotting were used to analyze the gene and protein expression of nuclear factor kappa-B (NF-κB) p65, inhibitor of kappa-B (IκB), inhibitor of NF-κB kinase (IκK-β), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α).
Results: Compared with the control group, the rats in each treatment group showed general improvements in hemorrhoidal tissue pathology. The AAFL group showed increased IκB expression and decreased IL-1β, IL-6, TNF-α, NF-κB, p65, and IκK-β expressions.
Conclusion: AAFL can decrease the production of inflammatory markers by targeting the NF-κB pathway, resulting in improved pathological conditions in mixed hemorrhoids. Our findings will aid in the treatment of mixed hemorrhoids.
目的：探讨安氏肛肠熏洗液治疗炎性混合痔的作用机制。方法：取80只雌雄相等的Sprague-Dawley大鼠随机分为对照组、模型组、AAFL组和阳性组。痔疮诱导后，取大鼠痔疮组织进行分析。通过苏木精-伊红染色检查这些组织的病理改变。免疫组织化学检测炎症标志物。透射电镜观察各组组织超微结构病理改变。采用逆转录聚合酶链反应和Western blotting检测核因子κ b (NF-κB) p65、κ b抑制剂（i -κB）、NF-κB激酶抑制剂（i -κ k -β）、白细胞介素-1β (IL-1β)、白细胞介素-6 （IL-6）、肿瘤坏死因子-α (TNF-α)的基因和蛋白表达。结果：与对照组比较，各治疗组大鼠痔疮组织病理均有改善。AAFL组i -κB表达升高，IL-1β、IL-6、TNF-α、NF-κB、p65、i -κ k -β表达降低。结论：AAFL可通过靶向NF-κB通路，减少炎症标志物的产生，改善混合痔的病理状况。我们的发现将有助于治疗混合性痔疮。
{"title":"An's anorectal fumigation lotion for nuclear factor kappa-B pathway-targeted treatment of inflammatory mixed hemorrhoids.","authors":"Feng Dayong, A N Ayue, Feng Yuening, Wang Chunhui, Bai Zhiyong, Wang Qian","doi":"10.19852/j.cnki.jtcm.2025.04.008","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.008","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism by which An's anorectal fumigation lotion (AAFL) treats inflammatory mixed hemorrhoids.</p><p><strong>Methods: </strong>Eighty Sprague-Dawley rats, with an equal number of males and females, were randomly assigned to the following four groups: control, model, AAFL, and positive groups. Following hemorrhoid induction, hemorrhoidal tissues were collected from the rats for analysis. Pathological alterations in these tissues were examined <i>via</i> hematoxylin-eosin staining. Immun-ohistochemistry was used to detect inflammatory markers. The ultrastructural pathological changes in these tissues were observed by transmission electron microscopy. Reverse transcription-polymerase chain reaction and Western blotting were used to analyze the gene and protein expression of nuclear factor kappa-B (NF-κB) p65, inhibitor of kappa-B (IκB), inhibitor of NF-κB kinase (IκK-β), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α).</p><p><strong>Results: </strong>Compared with the control group, the rats in each treatment group showed general improvements in hemorrhoidal tissue pathology. The AAFL group showed increased IκB expression and decreased IL-1β, IL-6, TNF-α, NF-κB, p65, and IκK-β expressions.</p><p><strong>Conclusion: </strong>AAFL can decrease the production of inflammatory markers by targeting the NF-κB pathway, resulting in improved pathological conditions in mixed hemorrhoids. Our findings will aid in the treatment of mixed hemorrhoids.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"777-785"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.003
Fan Changzheng, Zhang Qiong, Fan Maorong, Meng Hongxu, Cong Xiaodong, Fan Yiling, Yuan Shasha, Miao Qing
Objective: To explore the role and mechanism of Qufeng Jiejing (，QFJJ) formula in the asthma progression.
Methods: The Bagg Albino/c mice treated with Ovalbumin and AL(OH)3, and airway smooth muscle cells (ASMCs) treated with platelet-derived growth factor (PDGF)-BB to establish a asthma model in vivo and in vitro. The cell morphology was observed with microscope and immunofluorescence staining. The cell viability was assessed with methyl thiazolyl tetrazolium assay. The tumor necrosis factor-αlpha (TNF-α), interleukin-1beta (IL-1β), laminin, fibronectin and collagen IV levels in the ASMCs were detected with corresponding enzyme linked immunosorbent assay kits. Transwell and wound healing assays were conducted to test the cell migration. The TGF-β1, Smad2 and Smad3 levels were measured with Western blot.
Results: We found that QFJJ formula treatment dramatically decreased the cell viability, TNF-α, IL-1β, laminin, fibronectin and collagen IV levels in the PDGF-BB stimulated ASMCs. Additionally, the protein levels of TGF-β1, Smad2 and Smad3 in the PDGF-BB stimulated ASMCs were prominently depleted after QFJJ formula treatment. Besides, SRI treatment neutralized the role of QFJJ formula in the PDGF-BB stimulated ASMCs.
Conclusion: QFJJ formula effectively relieved the asthma progression through ameliorate the ASMCs function, which was achieved through suppressing the TGF-β1/Smads signaling pathway.
{"title":"Qufeng Jiejing formula ameliorated the injury of airway smooth muscle cells induced by platelet-derived growth factor-BB through the transforming growth factor-β1/Smads signaling pathway.","authors":"Fan Changzheng, Zhang Qiong, Fan Maorong, Meng Hongxu, Cong Xiaodong, Fan Yiling, Yuan Shasha, Miao Qing","doi":"10.19852/j.cnki.jtcm.2025.04.003","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.003","url":null,"abstract":"<p><strong>Objective: </strong>To explore the role and mechanism of Qufeng Jiejing (，QFJJ) formula in the asthma progression.</p><p><strong>Methods: </strong>The Bagg Albino/c mice treated with Ovalbumin and AL(OH)3, and airway smooth muscle cells (ASMCs) treated with platelet-derived growth factor (PDGF)-BB to establish a asthma model in vivo and in vitro. The cell morphology was observed with microscope and immunofluorescence staining. The cell viability was assessed with methyl thiazolyl tetrazolium assay. The tumor necrosis factor-αlpha (TNF-α), interleukin-1beta (IL-1β), laminin, fibronectin and collagen IV levels in the ASMCs were detected with corresponding enzyme linked immunosorbent assay kits. Transwell and wound healing assays were conducted to test the cell migration. The TGF-β1, Smad2 and Smad3 levels were measured with Western blot.</p><p><strong>Results: </strong>We found that QFJJ formula treatment dramatically decreased the cell viability, TNF-α, IL-1β, laminin, fibronectin and collagen IV levels in the PDGF-BB stimulated ASMCs. Additionally, the protein levels of TGF-β1, Smad2 and Smad3 in the PDGF-BB stimulated ASMCs were prominently depleted after QFJJ formula treatment. Besides, SRI treatment neutralized the role of QFJJ formula in the PDGF-BB stimulated ASMCs.</p><p><strong>Conclusion: </strong>QFJJ formula effectively relieved the asthma progression through ameliorate the ASMCs function, which was achieved through suppressing the TGF-β1/Smads signaling pathway.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"730-738"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.021
Long Xi, W U Zixuan, Y U Yunfeng, Lin Jie, Peng Qinghua
<p><strong>Objective: </strong>To utilize the Traditional Chinese Medicine constitution (TCMC) as a complementary and alternative approach for early disease detection and treatment, with a focus on <i>Yin</i> and <i>Yang</i> deficiency constitutions, which serve as key references for disease prevention and management.</p><p><strong>Methods: </strong>The dataset containing the data of <i>Yin</i> and <i>Yang</i> deficiency constitution was identified through the Gene Expression Omnibus database. This database was used for differential expression genes (DEGs) analysis and weighted gene co-expression network analysis (WGCNA), and the characteristic genes were then obtained in the dataset using a machine learning method. The hub genes of <i>Yin</i> and <i>Yang</i> deficiency constitution were obtained after analysis using the above three methods, and the hub genes were enriched and analyzed. Subsequently, the hub genes of <i>Yin</i> and <i>Yang</i> deficiency constitution were validated using external datasets. Receiver operating characteristic (ROC) analysis was used on each hub genes of the two groups to further understand their diagnostic performance. The miRNA-lncRNA-gene network was used to further analyze the hub genes. Immunoinfiltration and gene set enrichment analysis were performed on the shared hub genes.</p><p><strong>Results: </strong>The GSE87474 dataset was used for DEGs analysis and WGCNA. Using machine learning analyses, we identified 15 and 14 hub genes for <i>Yin</i> and <i>Yang</i> deficiency constitutions, respectively. The results of enrichment analyses showed that <i>Yin</i> deficiency constitution was associated with interleukin-17 signaling pathway, whereas <i>Yang</i> deficiency constitution was associated with glycosaminoglycan biosynthesis-keratan sulfate. The validation dataset GSE56116 showed statistically significant data for s-adenosylmethionine sensor upstream of MTORC1 (SAMTOR, also named C7orf60), cofilin 2 (CFL2), cytohesin 1 interacting protein (CYTIP), G protein-coupled receptor 183 (GPR183), hippocampus abundant transcript 1 (HIAT1), kelch like family member 15 (KLHL15), mitogen-activated protein kinase 6 (MAPK6), and prostaglandin-endoperoxide synthase 2 (PTGS2) in <i>Yin</i> deficiency and fucosy-ltransferase 8 (FUT8), TATA-box binding protein associated factor, RNA polymerase I subunit D (TAF1D), zinc finger protein 24 (ZNF24), MAPK6, and leptin receptor overlapping transcript like 1 (LEPROTL1) in <i>Yang</i> deficiency. The ROC results indicated that these genes have diagnostic value. MAPK6 is a shared hub gene for <i>Yin</i> and <i>Yang</i> deficiencies.</p><p><strong>Conclusions: </strong>This study identified C7orf60, CFL2, CYTIP, GPR183, HIAT1, KLHL15, MAPK6, and PTGS2 in <i>Yin</i> deficiency and FUT8, TAF1D, ZNF24, MAPK6, and LEPROTL1 in <i>Yang</i> deficiency as potential biomarkers, providing insights into their pathogenesis. This theory not only guides the diagnostic approach in TCM but also extends
{"title":"Identification of characteristic genes ofanddeficiency constitutions: an integrated analysis based on bioinformatics and machine learning.","authors":"Long Xi, W U Zixuan, Y U Yunfeng, Lin Jie, Peng Qinghua","doi":"10.19852/j.cnki.jtcm.2025.04.021","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.021","url":null,"abstract":"<p><strong>Objective: </strong>To utilize the Traditional Chinese Medicine constitution (TCMC) as a complementary and alternative approach for early disease detection and treatment, with a focus on <i>Yin</i> and <i>Yang</i> deficiency constitutions, which serve as key references for disease prevention and management.</p><p><strong>Methods: </strong>The dataset containing the data of <i>Yin</i> and <i>Yang</i> deficiency constitution was identified through the Gene Expression Omnibus database. This database was used for differential expression genes (DEGs) analysis and weighted gene co-expression network analysis (WGCNA), and the characteristic genes were then obtained in the dataset using a machine learning method. The hub genes of <i>Yin</i> and <i>Yang</i> deficiency constitution were obtained after analysis using the above three methods, and the hub genes were enriched and analyzed. Subsequently, the hub genes of <i>Yin</i> and <i>Yang</i> deficiency constitution were validated using external datasets. Receiver operating characteristic (ROC) analysis was used on each hub genes of the two groups to further understand their diagnostic performance. The miRNA-lncRNA-gene network was used to further analyze the hub genes. Immunoinfiltration and gene set enrichment analysis were performed on the shared hub genes.</p><p><strong>Results: </strong>The GSE87474 dataset was used for DEGs analysis and WGCNA. Using machine learning analyses, we identified 15 and 14 hub genes for <i>Yin</i> and <i>Yang</i> deficiency constitutions, respectively. The results of enrichment analyses showed that <i>Yin</i> deficiency constitution was associated with interleukin-17 signaling pathway, whereas <i>Yang</i> deficiency constitution was associated with glycosaminoglycan biosynthesis-keratan sulfate. The validation dataset GSE56116 showed statistically significant data for s-adenosylmethionine sensor upstream of MTORC1 (SAMTOR, also named C7orf60), cofilin 2 (CFL2), cytohesin 1 interacting protein (CYTIP), G protein-coupled receptor 183 (GPR183), hippocampus abundant transcript 1 (HIAT1), kelch like family member 15 (KLHL15), mitogen-activated protein kinase 6 (MAPK6), and prostaglandin-endoperoxide synthase 2 (PTGS2) in <i>Yin</i> deficiency and fucosy-ltransferase 8 (FUT8), TATA-box binding protein associated factor, RNA polymerase I subunit D (TAF1D), zinc finger protein 24 (ZNF24), MAPK6, and leptin receptor overlapping transcript like 1 (LEPROTL1) in <i>Yang</i> deficiency. The ROC results indicated that these genes have diagnostic value. MAPK6 is a shared hub gene for <i>Yin</i> and <i>Yang</i> deficiencies.</p><p><strong>Conclusions: </strong>This study identified C7orf60, CFL2, CYTIP, GPR183, HIAT1, KLHL15, MAPK6, and PTGS2 in <i>Yin</i> deficiency and FUT8, TAF1D, ZNF24, MAPK6, and LEPROTL1 in <i>Yang</i> deficiency as potential biomarkers, providing insights into their pathogenesis. This theory not only guides the diagnostic approach in TCM but also extends ","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"909-921"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-01DOI: 10.19852/j.cnki.jtcm.2025.04.004
Shi Jinyu, G E Haiya, Yang Zongrui, Zhan Hongsheng
Objective: To explore the potential molecular mechanism of Qigu capsule (，QGC) improve the functional performance of skeletal muscle.
Methods: The primary components of QGC were analyzed using high-performance liquid chromatography (HPLC). Muscle dysfunction was established in male C57BL/6 mice treated with dexamethasone (1 mg/kg body weight, i.p., six weeks). Rotarod test, mitochondrial ultrastructure, respiratory chain complex V activity, succinate dehydrogenase (SDH) activity, adenosine triphosphate (ATP) content, and reactive oxygen species (ROS) levels were assessed. The mitochondrial biogenesis-related protein expressions were analyzed using Western blot or polymerase chain reaction (PCR).
Results: QGC treatment enhanced Rotarod test performance. Additionally, QGC significantly alleviated dexamethasone-induced mitochondrial damage, reduced mitochondrial swelling, increased respiratory chain complex enzyme activity, SDH activity, ATP content, and decreased ROS levels. PCR and western blot results revealed that QGC enhanced mitochondrial biogenesis via adenosine 5'-monophosphate-activated protein kinase (AMPK)/peroxisome proliferator-activated receptor-γ coactivator 1-alpha (PGC-1α) signaling pathway.
Conclusions: QGC ameliorates dexamethasone-induced skeletal muscle dysfunction by activating AMPK/ PGC-1α, which might be developed as a therapeutic agent for treating age-related muscle weakness.
{"title":"Qigu capsule alleviates dexamethasone-induced muscle dysfunction through mitochondrial biogenesis.","authors":"Shi Jinyu, G E Haiya, Yang Zongrui, Zhan Hongsheng","doi":"10.19852/j.cnki.jtcm.2025.04.004","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.004","url":null,"abstract":"<p><strong>Objective: </strong>To explore the potential molecular mechanism of Qigu capsule (，QGC) improve the functional performance of skeletal muscle.</p><p><strong>Methods: </strong>The primary components of QGC were analyzed using high-performance liquid chromatography (HPLC). Muscle dysfunction was established in male C57BL/6 mice treated with dexamethasone (1 mg/kg body weight, i.p., six weeks). Rotarod test, mitochondrial ultrastructure, respiratory chain complex V activity, succinate dehydrogenase (SDH) activity, adenosine triphosphate (ATP) content, and reactive oxygen species (ROS) levels were assessed. The mitochondrial biogenesis-related protein expressions were analyzed using Western blot or polymerase chain reaction (PCR).</p><p><strong>Results: </strong>QGC treatment enhanced Rotarod test performance. Additionally, QGC significantly alleviated dexamethasone-induced mitochondrial damage, reduced mitochondrial swelling, increased respiratory chain complex enzyme activity, SDH activity, ATP content, and decreased ROS levels. PCR and western blot results revealed that QGC enhanced mitochondrial biogenesis <i>via</i> adenosine 5'-monophosphate-activated protein kinase (AMPK)/peroxisome proliferator-activated receptor-γ coactivator 1-alpha (PGC-1α) signaling pathway.</p><p><strong>Conclusions: </strong>QGC ameliorates dexamethasone-induced skeletal muscle dysfunction by activating AMPK/ PGC-1α, which might be developed as a therapeutic agent for treating age-related muscle weakness.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"739-746"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the site specificity and differences of the Hegu (LI4) located on the midpoint of the second metacarpal and the point where the bases of the first and second metacarpal bones on the dorsum was historically used as Hegu (LI4) in Japan, through morphological, biomechanical, and histological analyses.
Methods: We defined distally located Hegu (LI4) as Hegu (LI4)-D and proximally located Hegu (LI4) as Hegu (LI4)-P. The distance from the skin surface to the blood vessels as well as the biomechanical properties of the two Hegu (LI4) samples were measured using an ultrasound device and MyotonPRO (MyotonAS, Tallinn, Estonia), respectively, in 20 healthy adult volunteers. Sympathetic fibers under the two Hegu (LI4) samples were histologically observed using tyrosine hydroxylase (TH) immunostaining in 10 cadavers.
Results: Hegu (LI4)-D was classified into type 1 with blood vessels < 4 mm away from the skin surface, and type 2 with blood vessels > 10 mm away from the skin surface. In Hegu (LI4)-P, blood vessels were approximately 10 mm away from the surface. Further, Hegu (LI4)-P had significantly higher tone, stiffness, and elasticity than Hegu (LI4)-D (P < 0.0001). TH-positive fibers were present near the artery in Hegu (LI4)-P.
Conclusion: Hegu (LI4)-D and Hegu (LI4)-P differed in terms of hardness and the distance between the skin surface and blood vessels. Furthermore, sympathetic nerve fibers were present near the artery in Hegu (LI4)-P. Taken together, these results suggest that there is site specificity of morphological, biomechanical, and histological differences between the Hegu (LI4)-D and Hegu (LI4)-P.
{"title":"Novel insight into the site-specificity of Hegu (LI4): morphological, biomechanical, and histological analyses.","authors":"Kanae Umemoto, Shan Xiyao, Takuro Ishikawa, Tadashi Watsuji, Yasuharu Watanabe, Munekazu Naito","doi":"10.19852/j.cnki.jtcm.2025.04.016","DOIUrl":"10.19852/j.cnki.jtcm.2025.04.016","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the site specificity and differences of the Hegu (LI4) located on the midpoint of the second metacarpal and the point where the bases of the first and second metacarpal bones on the dorsum was historically used as Hegu (LI4) in Japan, through morphological, biomechanical, and histological analyses.</p><p><strong>Methods: </strong>We defined distally located Hegu (LI4) as Hegu (LI4)-D and proximally located Hegu (LI4) as Hegu (LI4)-P. The distance from the skin surface to the blood vessels as well as the biomechanical properties of the two Hegu (LI4) samples were measured using an ultrasound device and MyotonPRO (MyotonAS, Tallinn, Estonia), respectively, in 20 healthy adult volunteers. Sympathetic fibers under the two Hegu (LI4) samples were histologically observed using tyrosine hydroxylase (TH) immunostaining in 10 cadavers.</p><p><strong>Results: </strong>Hegu (LI4)-D was classified into type 1 with blood vessels < 4 mm away from the skin surface, and type 2 with blood vessels > 10 mm away from the skin surface. In Hegu (LI4)-P, blood vessels were approximately 10 mm away from the surface. Further, Hegu (LI4)-P had significantly higher tone, stiffness, and elasticity than Hegu (LI4)-D (<i>P</i> < 0.0001). TH-positive fibers were present near the artery in Hegu (LI4)-P.</p><p><strong>Conclusion: </strong>Hegu (LI4)-D and Hegu (LI4)-P differed in terms of hardness and the distance between the skin surface and blood vessels. Furthermore, sympathetic nerve fibers were present near the artery in Hegu (LI4)-P. Taken together, these results suggest that there is site specificity of morphological, biomechanical, and histological differences between the Hegu (LI4)-D and Hegu (LI4)-P.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 4","pages":"867-872"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144850173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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