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Efficacy of Sailuotong on neurovascular unit in amyloid precursor protein/presenilin-1 transgenic mice with Alzheimer's disease. 赛络通对淀粉样前体蛋白/presenilin-1转基因阿尔茨海默病小鼠神经血管单元的疗效
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20240203.007
Sun Linjuan, L I Chengfu, Liu Jiangang, L I Nannan, Han Fuhua, Qiao Dandan, Tao Zhuang, Zhan Min, Chen Wenjie, Zhang Xiaohui, Tong Chenguang, Chen Dong, Qi Jiangxia, Liu Yang, Liang Xiao, Zheng Xiaoying, Zhang Yunling

Objective: To discuss the influence of Sailuotong (, SLT) on the Neurovascular Unit (NVUs) of amyloid precursor protein (APP)/presenilin-1(PS1) mice and evaluate the role of gas supplementation in activating blood circulation during the progression of Alzheimer's disease (AD).

Methods: The mice were allocated into the following nine groups: (a) the C57 Black (C57BL) sham-operated group (control group), (b) ischaemic treatment in C57BL mice (the C57 ischaemic group), (c) the APP/PS1 sham surgery group (APP/PS1 model group), (d) ischaemic treatment in APP/PS1 mice (APP/PS1 ischaemic group), (e) C57BL mice treated with aspirin following ischaemic treatment (C57BL ischaemic + aspirin group), (f) C57BL mice treated with SLT following ischaemic treatment (C57BL ischaemic + SLT group), (g) APP/PS1 mice treated with SLT (APP/PS1 + SLT group), (h) APP/PS1 mice treated with donepezil hydrochloride following ischaemic treatment (APP/PS1 ischaemic + donepezil hydrochloride group) and (i) APP/PS1 mice treated with SLT following ischaemic treatment (APP/PS1 ischaemic + SLT group). The ischaemic model was established by operating on the bilateral common carotid arteries and creating a microembolism. The Morris water maze and step-down tests were used to detect the spatial behaviour and memory ability of mice. The hippocampus of each mouse was observed by haematoxylin and eosin (HE) and Congo red staining. The ultrastructure of NVUs in each group was observed by electron microscopy, and various biochemical indicators were detected by enzyme-linked immunosorbent assay (ELISA). The protein expression level was detected by Western blot. The mRNA expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: The results of the Morris water maze and step-down tests showed that ischemia reduced learning and memory in the mice, which were restored by SLT. The results of HE staining showed that SLT restored the pathological changes of the NVUs. The Congo red staining results revealed that SLT also improved the scattered orange-red sediments in the upper cortex and hippocampus of the APP/PS1 and APP/PS1 ischaemic mice. Furthermore, SLT significantly reduced the content of Aβ, improved the vascular endothelium and repaired the mitochondrial structures. The ELISA detection, western blot detection and qRT-PCR showed that SLT significantly increased the vascular endothelial growth factor (VEGF), angiopoietin and basic fibroblast growth factor, as well as the levels of gene and protein expression of low-density lipoprotein receptor-related protein-1 (LRP-1) and VEGF in brain tissue.

Conclusions: By increasing the expression of VEGF, SLT can promote vascular proliferation, up-regulate the expression of LRP-1, promote the clearance of Aβ and improve the cognitive impairment of APP/PS1 mice. These results confirm that SLT can improve AD by promoting vascular

目的探讨赛络通(SLT)对淀粉样前体蛋白(APP)/脑啡肽-1(PS1)小鼠神经血管单元(NVU)的影响,并评估在阿尔茨海默病(AD)进展过程中补气活血的作用:将小鼠分为以下九组:(方法:将小鼠分为以下九组:(a)C57 黑(C57BL)假手术组(对照组);(b)C57BL 小鼠缺血治疗组(C57 缺血组);(c)APP/PS1 假手术组(APP/PS1 模型组)、(d) APP/PS1 小鼠缺血治疗(APP/PS1 缺血组), (e) C57BL 小鼠缺血治疗后使用阿司匹林治疗(C57BL 缺血 + 阿司匹林组)、(f) C57BL 小鼠缺血后接受 SLT 治疗(C57BL 缺血 + SLT 组), (g) APP/PS1 小鼠接受 SLT 治疗(APP/PS1 + SLT 组)、(h) APP/PS1 小鼠缺血后用盐酸多奈哌齐治疗(APP/PS1 缺血 + 盐酸多奈哌齐组)和 (i) APP/PS1 小鼠缺血后用 SLT 治疗(APP/PS1 缺血 + SLT 组)。缺血模型是通过在双侧颈总动脉上操作并产生微栓子而建立的。采用莫里斯水迷宫和降阶测试检测小鼠的空间行为和记忆能力。对每只小鼠的海马进行了血色素和伊红(HE)以及刚果红染色观察。用电子显微镜观察各组 NVU 的超微结构,并用酶联免疫吸附试验(ELISA)检测各种生化指标。蛋白表达水平通过 Western 印迹法检测。结果:结果:Morris水迷宫和降阶试验结果表明,缺血会降低小鼠的学习和记忆能力,而SLT可恢复小鼠的学习和记忆能力。HE 染色结果显示,SLT 恢复了 NVU 的病理变化。刚果红染色结果显示,SLT 还改善了 APP/PS1 和 APP/PS1 缺血小鼠上皮层和海马中分散的橙红色沉淀物。此外,SLT 还能明显降低 Aβ 含量,改善血管内皮,修复线粒体结构。ELISA检测、Western印迹检测和qRT-PCR检测表明,SLT能显著提高脑组织中血管内皮生长因子(VEGF)、血管生成素和碱性成纤维细胞生长因子的含量,以及低密度脂蛋白受体相关蛋白-1(LRP-1)和血管内皮生长因子的基因和蛋白表达水平:结论:通过增加血管内皮生长因子的表达,SLT 可以促进血管增殖,上调 LRP-1 的表达,促进 Aβ 的清除,改善 APP/PS1 小鼠的认知功能障碍。这些结果证实,SLT可通过促进血管增殖和Aβ清除来保护NVU的功能,从而改善AD。
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引用次数: 0
Yemazhui () ameliorates lipopolysaccharide-induced acute lung injury modulation of the toll-like receptor 4/nuclear factor kappa-B/nod-like receptor family pyrin domain-containing 3 protein signaling pathway and intestinal flora in rats. 叶马肼()可改善脂多糖诱导的大鼠急性肺损伤,调节toll样受体4/核因子卡巴-B/nod样受体家族含吡啶结构域的3蛋白信号通路和肠道菌群。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20230510.001
Ren Li, Hai Yang, Yang Xue, Luo Xianqin

Objective: To investigate the impact of Yemazhui (Herba Eupatorii Lindleyani, HEL) against lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its underlying mechanism in vivo.

Methods: The chemical constituents of HEL were analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry method. Then, HEL was found to suppress LPS-induced ALI in vivo. Six-week-old male Sprague-Dawley rats were randomly divided into 6 groups: control, LPS, Dexamethasone (Dex), HEL low dose 6 g/kg (HEL-L), HEL medium dose 18 g/kg (HEL-M) and HEL high dose 54 g/kg (HEL-H) groups. The model rats were intratracheally injected with 3 mg/kg LPS to establish an ALI model. Leukocyte counts, lung wet/dry weight ratio, as well as myeloperoxidase (MPO) activity were determined followed by the detection with hematoxylin and eosin staining, enzyme linked immunosorbent assay, quantitative real time polymerase chain reaction, western blotting, immunohistochemistry, and immunofluorescence. Besides, to explore the effect of HEL on ALI-mediated intestinal flora, we performed 16s rRNA sequencing analysis of intestinal contents.

Results: HEL attenuated LPS-induced inflammation in lung tissue and intestinal flora disturbance. Mechanism study indicated that HEL suppressed the lung coefficient and wet/dry weight ratio of LPS-induced ALI in rats, inhibited leukocytes exudation and MPO activity, and improved the pathological injury of lung tissue. In addition, HEL reduced the expression of tumor necrosis factor-alpha, interleukin-1beta (IL-1β) and interleukin-6 (IL-6) in bronchoalveolar lavage fluid and serum, and inhibited nuclear displacement of nuclear factor kappa-B p65 (NF-κBp65). And 18 g/kg HEL also reduced the expression levels of toll-like receptor 4 (TLR4), myeloid differentiation factor 88, NF-κBp65, phosphorylated inhibitor kappa B alpha (phospho-IκBα), nod-like receptor family pyrin domain-containing 3 protein (NLRP3), IL-1β, and interleukin-18 (IL-18) in lung tissue, and regulated intestinal flora disturbance.

Conclusions: In summary, our findings revealed that HEL has a protective effect on LPS-induced ALI in rats, and its mechanism may be related to inhibiting TLR4/ NF-κB/NLRP3 signaling pathway and improving intestinal flora disturbance.

目的方法:采用超高效液相色谱-四极杆飞行时间质谱法分析叶下珠的化学成分:方法:采用超高效液相色谱-四极杆飞行时间质谱法分析了HEL的化学成分。方法:采用超高效液相色谱-ququole飞行时间质谱法分析了 HEL 的化学成分,发现 HEL 能抑制 LPS 诱导的体内 ALI。将六周大的雄性 Sprague-Dawley 大鼠随机分为 6 组:对照组、LPS 组、地塞米松组(Dex)、HEL 低剂量 6 g/kg 组(HEL-L)、HEL 中剂量 18 g/kg 组(HEL-M)和 HEL 高剂量 54 g/kg 组(HEL-H)。向模型大鼠气管内注射 3 毫克/千克 LPS 以建立 ALI 模型。通过苏木精和伊红染色、酶联免疫吸附试验、定量实时聚合酶链反应、Western印迹、免疫组织化学和免疫荧光等方法检测白细胞计数、肺干湿重比和髓过氧化物酶(MPO)活性。此外,为了探讨 HEL 对 ALI 介导的肠道菌群的影响,我们对肠道内容物进行了 16s rRNA 测序分析:结果:HEL减轻了LPS诱导的肺组织炎症和肠道菌群紊乱。机理研究表明,HEL可抑制LPS诱导的大鼠ALI的肺系数和干湿重比,抑制白细胞渗出和MPO活性,改善肺组织的病理损伤。此外,HEL 还能降低支气管肺泡灌洗液和血清中肿瘤坏死因子-α、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的表达,抑制核因子卡巴-B p65(NF-κBp65)的核移位。18 g/kg HEL还能降低肺组织中toll样受体4(TLR4)、髓样分化因子88、NF-κBp65、磷酸化抑制因子kappa B alpha(phospho-IκBα)、nod样受体家族含吡咯啉结构域的3蛋白(NLRP3)、IL-1β和白细胞介素-18(IL-18)的表达水平,并调节肠道菌群紊乱:综上所述,我们的研究结果表明,HEL对LPS诱导的大鼠ALI具有保护作用,其机制可能与抑制TLR4/ NF-κB/NLRP3信号通路和改善肠道菌群紊乱有关。
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引用次数: 0
Mechanism of acupuncture in attenuating cerebral ischaemia-reperfusion injury based on nuclear receptor coactivator 4 mediated ferritinophagy. 基于核受体辅激活子4介导的铁蛋白吞噬作用的针灸减轻脑缺血再灌注损伤的机制
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20240203.006
Zhang Xinchang, Huang Zheng, Huang Peiyan, Yang Mengning, Zhang Zhihui, N I Guangxia

Objective: To explore the effect of acupuncture treatment on cerebral ischaemia-reperfusion injury (CIRI) and reveal the underlying mechanism of the effect based on nuclear receptor coactivator 4 (NCOA4) mediated ferritinophagy.

Methods: Sprague-Dawley male rats were divided into four groups: the sham group, model group, acupuncture group, and sham acupuncture group. After 2 h of middle cerebral artery occlusion (MCAO), reperfusion was performed for 24 h to induce CIRI. The rats were treated with acupuncture at the Neiguan (PC6) and Shuigou (GV26) acupoints. Their neurological function was evaluated by taking their Bederson scores at 2 h after ischaemia and 24 h after reperfusion. Triphenyltetrazolium chloride staining was applied to assess the cerebral infarct volume at 24 h after reperfusion. The malondialdehyde (MDA) and ferrous iron (Fe2+) levels were observed after 24 h of reperfusion using an assay kit. Western blotting was performed to detect the expression of NCOA4 and ferritin heavy chain 1 (FTH1) at 24 h after reperfusion. Moreover, the colocalization of ferritin with neurons, NCOA4 with microtubule-associated protein 1 light chain 3 (LC3), and NCOA4 with ferritin was visualized using immunofluorescence staining.

Results: Acupuncture significantly improved neurological function and decreased cerebral infarct volume in the acupuncture group. Following CIRI, the expression of NCOA4, LC3 and FTH1 was increased, which enhanced ferritinophagy and induced an inappropriate accumulation of Fe2+ and MDA in the ischaemic brain. However, acupuncture dramatically downregulated the expression of NCOA4, LC3 and FTH1, inhibited the overactivation of ferritinophagy, and decreased the levels of MDA and Fe2+.

Conclusions: Acupuncture can inhibit NCOA4-mediated ferritinophagy and protect neurons against CIRI in a rat model.

目的探讨针刺治疗对脑缺血再灌注损伤(CIRI)的影响,并揭示基于核受体辅激活子4(NCOA4)介导的噬铁蛋白作用的内在机制:方法:将Sprague-Dawley雄性大鼠分为四组:假组、模型组、针刺组和假针刺组。大脑中动脉闭塞(MCAO)2 小时后,再灌注 24 小时诱导 CIRI。针刺大鼠的内关(PC6)和水沟(GV26)穴位。缺血 2 小时后和再灌注 24 小时后,分别对大鼠的神经功能进行贝德森评分。三苯基氯化四氮唑染色用于评估再灌注后 24 小时的脑梗塞体积。再灌注 24 小时后,使用检测试剂盒观察丙二醛(MDA)和亚铁(Fe2+)水平。在再灌注 24 小时后,用 Western 印迹法检测 NCOA4 和铁蛋白重链 1 (FTH1) 的表达。此外,还利用免疫荧光染色法检测了铁蛋白与神经元、NCOA4与微管相关蛋白1轻链3(LC3)以及NCOA4与铁蛋白的共定位:结果:针刺组患者的神经功能明显改善,脑梗死体积明显缩小。CIRI 后,NCOA4、LC3 和 FTH1 的表达增加,从而增强了铁蛋白吞噬作用,诱导缺血脑内 Fe2+ 和 MDA 的不适当积累。然而,针刺能显著降低 NCOA4、LC3 和 FTH1 的表达,抑制噬铁细胞的过度激活,降低 MDA 和 Fe2+ 的水平:结论:针灸可抑制 NCOA4 介导的噬铁蛋白作用,保护大鼠神经元免受 CIRI 损伤。
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引用次数: 0
Protective effect of modified Huangqi Chifeng decoction on immunoglobulin A nephropathy through toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B signaling pathway. 改良黄芪赤峰煎剂通过toll样受体4/髓系分化因子88/核因子-kappa B信号通路对免疫球蛋白A肾病的保护作用
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20240203.001
L I Liusheng, Zhao Mingming, Chang Meiying, S I Yuan, Zhao Jinning, Yang Bin, Zhang Yu

Objective: To examine the nephroprotective mechanism of modified Huangqi Chifeng decoction (, MHCD) in immunoglobulin A nephropathy (IgAN) rats.

Methods: To establish the IgAN rat model, the bovine serum albumin, lipopolysaccharide, and carbon tetrachloride 4 method was employed. The rats were then randomly assigned to the control, model, telmisartan, and high-, medium-, and low-dose MHCD groups, and were administered the respective treatments via intragastric administration for 8 weeks. The levels of 24-h urinary protein, serum creatinine (CRE), and blood urea nitrogen (BUN) were measured in each group. Pathological alterations were detected. IgA deposition was visualized through the use of immunofluorescence staining. The ultrastructure of the kidney was observed using a transmission electron microscope. The expression levels of interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and transforming growth factor-β1 (TGF-β1) were examined by immunohistochemistry and quantitative polymerase chain reaction. Levels of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor-kappa B (NF-κB) P65, were examined by immunohistochemistry, Western blotting, and quantitative polymerase chain reaction.

Results: The 24-h urine protein level in each group increased significantly at week 6, and worsen from then on. But this process can be reversed by treatments of telmisartan, and high-, medium-, and low-dose of MHCD, and these treatments did not affect renal function. Telmisartan, and high-, and medium-dose of MHCD reduced IgA deposition. Renal histopathology demonstrated the protective effect of high-, medium-, and low-dose of MHCD against kidney injury. The expression levels of MCP-1, IL-6, and TGF-β1 in kidney tissues were downregulated by low, medium and high doses of MHCD treatment. Additionally, treatment of low, medium and high doses of MHCD decreased the protein and mRNA levels of TLR4, MyD88, and NF-κB.

Conclusions: MHCD exerted nephroprotective effects on IgAN rats, and MHCD regulated the expressions of key targets in TLR4/MyD88/NF-κB signaling pathway, thereby alleviating renal inflammation by inhibiting MCP-1, IL-6 expressions, and ameliorating renal fibrosis by inhibiting TGF-β1 expression.

目的研究改良黄芪赤峰煎剂(MHCD)对免疫球蛋白A肾病(IgAN)大鼠的肾脏保护机制:方法:采用牛血清白蛋白、脂多糖和四氯化碳4法建立IgAN大鼠模型。然后将大鼠随机分配到对照组、模型组、替米沙坦组和高、中、低剂量 MHCD 组,并通过胃内给药给予相应的治疗,持续 8 周。测量各组 24 小时尿蛋白、血清肌酐 (CRE) 和血尿素氮 (BUN) 的水平。检测到病理改变。免疫荧光染色法可观察到 IgA 沉积。使用透射电子显微镜观察肾脏的超微结构。免疫组织化学和定量聚合酶链反应检测了白细胞介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)和转化生长因子-β1(TGF-β1)的表达水平。免疫组化、Western 印迹和定量聚合酶链反应检测了收费样受体 4(TLR4)、髓样分化因子 88(MyD88)和核因子-卡巴 B(NF-κB)P65 的水平:结果:各组患者的 24 小时尿蛋白水平在第 6 周时明显升高,并从那时起开始恶化。但替米沙坦和高、中、低剂量的 MHCD 治疗可逆转这一过程,且这些治疗不影响肾功能。替米沙坦和高、中剂量的 MHCD 可减少 IgA 沉积。肾组织病理学显示,高、中、低剂量的 MHCD 对肾损伤有保护作用。低、中、高剂量的 MHCD 可降低肾组织中 MCP-1、IL-6 和 TGF-β1 的表达水平。此外,低、中、高剂量的MHCD还能降低TLR4、MyD88和NF-κB的蛋白和mRNA水平:结论:MHCD对IgAN大鼠具有肾保护作用,MHCD调节TLR4/MyD88/NF-κB信号通路中关键靶点的表达,从而通过抑制MCP-1、IL-6的表达缓解肾脏炎症,通过抑制TGF-β1的表达改善肾脏纤维化。
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引用次数: 0
Effectiveness and safety of Qidong Huoxue decoction in treatment of acute lung injury and acute respiratory distress syndrome: a randomized, controlled trial. 芪冬藿香汤治疗急性肺损伤和急性呼吸窘迫综合征的有效性和安全性:随机对照试验。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.2024.02.003
Y U Zhengqiu, Y U Liuda, Chen Ye, L I Mingjing, Cai Wanru

Objective: To evaluate the efficacy of Qidong Huoxue decoction (,QDHX) in treating acute lung injury and acute respiratory distress syndrome (ALI/ARDS) when used as an adjunctive treatment.

Methods: ALI/ARDS patients admitted to our medical intensive care unit were randomly allocated to the control group or the QDHX group and received standard therapy. The QDHX group received QDHX (50 mL per day for 14 d) orally or via a gastric tube. The primary outcome was measured according to Traditional Chinese Medicine (TCM) syndrome scores, with partial pressure of oxygen/fraction of inspired oxygen (PaO2/FiO2) levels as the secondary outcome.

Results: A total of 73 patients completed the study (36 in the TCM and 37 in the conventional group), and their records were analyzed. After 14-d treatment, the TCM group showed a significant decrease in TCM syndrome scores (P < 0.05) and increased PaO2/FiO2 levels (P < 0.05). The therapeutic effect of integrated Chinese and western medicine was more significant than that of Western Medicine alone. No serious side effects were observed.

Conclusions: Our study results show that QDHX in combination with conventional drug therapy can significantly reduce some clinical symptoms in patients with ALI/ARDS.

目的评估祁东藿香正气水辅助治疗急性肺损伤和急性呼吸窘迫综合征(ALI/ARDS)的疗效:方法:本院内科重症监护室收治的急性肺损伤/急性呼吸窘迫综合征患者被随机分配到对照组或 QDHX 组,接受标准治疗。QDHX组通过口服或胃管接受QDHX(每天50毫升,共14天)治疗。主要结果根据中医综合征评分进行测量,氧分压/吸入氧分压(PaO2/FiO2)水平作为次要结果:共有 73 名患者完成了研究(中医组 36 人,常规组 37 人),并对他们的病历进行了分析。治疗 14 天后,中医组的中医综合征评分显著下降(P 0.05),PaO2/FiO2 水平显著升高(P 0.05)。中西医结合治疗效果比单纯西医治疗效果更显著。未观察到严重的副作用:我们的研究结果表明,QDHX 联合常规药物治疗可明显减轻 ALI/ARDS 患者的一些临床症状。
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引用次数: 0
Long-term efficacy and safety of Huangqi ()-based Traditional Chinese Medicine in diabetic peripheral neuropathy: a Meta-analysis of randomized controlled trials. 黄芪中药对糖尿病周围神经病变的长期疗效和安全性:随机对照试验的 Meta 分析。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.2024.02.004
Ping Jing, Hao Hongzheng, W U Zhenqi, Zou Meijuan, L I Zuojing, Cheng Gang

Objective: To assess the long-term effectiveness of Huangqi (Radix Astragali Mongolici, HQ)-based Traditional Chinese Medicine (TCM) in the treatment of diabetic peripheral neuropathy (DPN).

Methods: Nine databases were searched to retrieve available randomized controlled trials that compared HQ-based TCM and Western Medicines in the treatment of DPN. The methodological quality of the included studies was assessed using the Cochrane bias risk tool, and RevMan 5.4 was used for data analysis. The effect estimates of interest were risk ratio (RR), mean difference (MD) or standardized mean difference (SMD) with 95% confidence interval (CI).

Results: The results from 48 available studies assessing 3759 patients demonstrated that cases administered HQ-based TCM [RR = 1.30, 95% CI (1.21, 1.40), P < 0.000 01] or HQ-based TCM combined with Western Medicines [RR = 1.25, 95% CI (1.19, 1.31), P < 0.000 01] exhibited higher total efficacy rates than individuals who received Western Medicine alone. The results showed that the HQ-based TCM group had decreased Toronto Clinical Scoring System scores [MD =-1.50, 95% CI (-1.83, -1.17), P < 0.000 01], and reduced serum interleukin 6 [SMD = -0.57, 95% CI (-0.87, -0.27), P = 0.0002] and tumor necrosis factors-α levels [SMD = -0.60, 95% CI (-0.95, -0.25), P = 0.0009]. In addition, both HQ-based TCM and HQ-based TCM combined with Western Medicine increased nerve conduction velocity and decreased glycaemia compared with Western Medicine alone. In terms of blood lipids, oxidative stress and adverse drug reactions, there were no significant differences between the HQ-based TCM groups and the Western Medicine control group.

Conclusion: The current Meta-analysis revealed that HQ-based TCM yields higher efficacy and safety than Western Medicine alone for the treatment of DPN, although further well-designed RCTs are required to validate these findings.

目的评估以黄芪为基础的中药治疗糖尿病周围神经病变(DPN)的长期疗效:方法:检索了九个数据库,以检索现有的随机对照试验,这些试验比较了以黄芪为基础的中药和西药治疗 DPN 的效果。使用 Cochrane 偏倚风险工具评估了纳入研究的方法学质量,并使用 RevMan 5.4 进行了数据分析。效果估计值为风险比(RR)、平均差(MD)或标准化平均差(SMD)及 95% 置信区间(CI):48项研究共评估了3759名患者,结果表明,与单独接受西药治疗的患者相比,服用以HQ为基础的中药[RR = 1.30,95% CI (1.21,1.40),P < 0.000 01]或以HQ为基础的中药联合西药[RR = 1.25,95% CI (1.19,1.31),P < 0.000 01]的患者总有效率更高。结果显示,以 HQ 为基础的中药组降低了多伦多临床评分系统评分[MD =-1.50, 95% CI (-1.83, -1.17), P < 0.000 01],降低了血清白细胞介素 6 [SMD = -0.57, 95% CI (-0.87, -0.27), P = 0.0002]和肿瘤坏死因子-α水平[SMD = -0.60, 95% CI (-0.95, -0.25),P = 0.0009]。此外,与单纯西医治疗相比,以 HQ 为基础的中医治疗和以 HQ 为基础的中医治疗联合西医治疗均能提高神经传导速度并降低血糖。在血脂、氧化应激和药物不良反应方面,以 HQ 为基础的中药组与西药对照组之间没有显著差异:目前的 Meta 分析表明,以 HQ 为基础的中医药治疗 DPN 比单纯西药治疗具有更高的疗效和安全性,尽管还需要进一步设计良好的 RCT 来验证这些研究结果。
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引用次数: 0
Effectiveness and safety of Jiawei Xiaoyao pill in the treatment of premenstrual syndrome (liver depression, spleen deficiency, and blood-heat syndrome): a multi-center, randomized, placebo-controlled trial. 加味消渴丸治疗经前综合征(肝郁脾虚、血热证)的有效性和安全性:一项多中心、随机、安慰剂对照试验。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20231110.003
L I Xiyu, Yang Yanhong, Sun Jian, Nie Quanfang, Liu Lifen, L I Guifen, Y U Junping, Zhang Zhuangjin, X U Yi, Zou Ting, Shi Yun

Objective: To investigate the effectiveness and safety of Jiawei Xiaoyao pill (,JXP) in the treatment of symptoms associated with premenstrual syndrome (PMS).

Methods: A total of 144 regularly menstruating women with PMS were recruited at 8 sites in China from August 2017 to December 2018, and randomized to receive either a JXP or a matching placebo (12 g/d, 6 g twice a day) for 3 menstrual cycles. The primary indicator was the reduced Daily Record of Severity of Problems (DRSP) scores in the luteal phase after 3 months of treatment. The safety outcomes included clinical adverse events (AEs), adverse reactions (ARs), changes in vital signs, and laboratory tests.

Results: JXP surpassed the placebo in reducing DRSP scores (psychological/somatic dysfunction) in the luteal phase over 3 menstrual cycles of treatment (PFAS = 0.002, PPPS = 0.001). Additionally, there were no significant differences in the incidence of AEs, severe AEs, withdrawal due to AEs and ARs between the two groups (all P > 0.05), and no clinically significant adverse medical events related to the test drug observed.

Conclusions: JXP was superior to the placebo in relieving the symptoms associated with PMS, which signified that JXP may be effective, safe, and well-tolerated as an alternative therapy.

目的:探讨加味逍遥丸(JXP)治疗经前期综合征(PMS)相关症状的有效性和安全性:研究加味逍遥丸(JXP)治疗经前期综合征(PMS)相关症状的有效性和安全性:2017年8月至2018年12月,在中国的8个地点共招募了144名定期来月经的经前综合征女性,并随机接受JXP或匹配的安慰剂(12克/天,每天两次,每次6克)治疗3个月经周期。主要指标是治疗3个月后黄体期每日问题严重程度记录(DRSP)评分的降低。安全性结果包括临床不良事件(AE)、不良反应(AR)、生命体征变化和实验室检测:结果:在 3 个月经周期的治疗中,JXP 在降低黄体期 DRSP 评分(心理/症状功能障碍)方面优于安慰剂(PFAS = 0.002,PPPS = 0.001)。此外,两组间的AEs、严重AEs、因AEs和ARs而停药的发生率无显著差异(均P>0.05),也未观察到与试验药物相关的临床重大不良医疗事件:结论:JXP 在缓解经前综合征相关症状方面优于安慰剂,这表明 JXP 可作为一种有效、安全且耐受性良好的替代疗法。
{"title":"Effectiveness and safety of Jiawei Xiaoyao pill in the treatment of premenstrual syndrome (liver depression, spleen deficiency, and blood-heat syndrome): a multi-center, randomized, placebo-controlled trial.","authors":"L I Xiyu, Yang Yanhong, Sun Jian, Nie Quanfang, Liu Lifen, L I Guifen, Y U Junping, Zhang Zhuangjin, X U Yi, Zou Ting, Shi Yun","doi":"10.19852/j.cnki.jtcm.20231110.003","DOIUrl":"10.19852/j.cnki.jtcm.20231110.003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effectiveness and safety of Jiawei Xiaoyao pill (,JXP) in the treatment of symptoms associated with premenstrual syndrome (PMS).</p><p><strong>Methods: </strong>A total of 144 regularly menstruating women with PMS were recruited at 8 sites in China from August 2017 to December 2018, and randomized to receive either a JXP or a matching placebo (12 g/d, 6 g twice a day) for 3 menstrual cycles. The primary indicator was the reduced Daily Record of Severity of Problems (DRSP) scores in the luteal phase after 3 months of treatment. The safety outcomes included clinical adverse events (AEs), adverse reactions (ARs), changes in vital signs, and laboratory tests.</p><p><strong>Results: </strong>JXP surpassed the placebo in reducing DRSP scores (psychological/somatic dysfunction) in the luteal phase over 3 menstrual cycles of treatment (PFAS = 0.002, PPPS = 0.001). Additionally, there were no significant differences in the incidence of AEs, severe AEs, withdrawal due to AEs and ARs between the two groups (all <i>P</i> > 0.05), and no clinically significant adverse medical events related to the test drug observed.</p><p><strong>Conclusions: </strong>JXP was superior to the placebo in relieving the symptoms associated with PMS, which signified that JXP may be effective, safe, and well-tolerated as an alternative therapy.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Formulation of international standards of Chinese medicine technology: clinical practice guide of Chinese medicine for cough. 制定中医药技术国际标准:中医治疗咳嗽临床实践指南。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20231016.001
Fang Hanyu, Hong Zheng, L I Deming, Zhang Hongchun, Shi Yihang, L I Xiaojuan, Sun Zengtao, Chen Wei, Zhang Chuchu, Z U Yaqi

Objective: To formulate the first clinical practice guideline for the treatment of cough using Chinese medicine based on the grading of recommendations assessment, development, and evaluation (GRADE) systematic approach, including clinical evidence, evaluation of ancient literature, and expert consensus.

Methods: In the process of development, the regulation of "evidence-based, consensus-assisted, and empirical" was followed, and a comprehensive systematic approach of recommendation assessment, GRADE, evidence-based evaluation, expert consensus, and the Delphi method was used. In the process of guideline development, evidence-based evaluation of ancient literature was included for the first time, and clinical evidence was fully integrated with clinical expert consensus.

Results: The clinical practice guidelines for the treatment of cough with Chinese herbal medicine were developed after a comprehensive consideration of evidence-based evaluation and expert opinions. The guideline recommendations focused on recommending herbal compound decoctions and Chinese patent medicines for cough in different conditions. Based on the GRADE systematic approach, we conducted an evidence-based evaluation of the recommended Chinese patent medicines one by one; meanwhile, the expert consensus method was used to unify the recommendations of both.

Conclusion: Based on clinical evidence, ancient literature evaluation, and expert consensus, a clinical practice guideline for Traditional Chinese Medicine (TCM) in the treatment of cough was developed, providing the first current clinical practice guideline for domestic and foreign TCM and Western medicine practitioners, especially respiratory professionals at home and abroad.

目的:方法:基于建议分级评估、发展和评价(GRADE)系统方法,包括临床证据、古代文献评价和专家共识,制定首个中医治疗咳嗽的临床实践指南:在制定过程中,遵循 "循证、共识辅助、实证 "的原则,采用建议评估、GRADE、循证评价、专家共识、德尔菲法等综合系统方法。在指南制定过程中,首次纳入了古代文献的循证评价,并将临床证据与临床专家共识充分结合:结果:在综合考虑循证评价和专家意见后,制定了《中药治疗咳嗽临床实践指南》。指南建议重点推荐了中药复方煎剂和中成药治疗不同病症的咳嗽。基于GRADE系统方法,我们对推荐的中成药逐一进行了循证评价,同时采用专家共识法对两者的推荐意见进行了统一:结论:基于临床证据、古代文献评价和专家共识,制定了中医治疗咳嗽的临床实践指南,为国内外中医、西医,尤其是国内外呼吸专业人员提供了第一份现行的临床实践指南。
{"title":"Formulation of international standards of Chinese medicine technology: clinical practice guide of Chinese medicine for cough.","authors":"Fang Hanyu, Hong Zheng, L I Deming, Zhang Hongchun, Shi Yihang, L I Xiaojuan, Sun Zengtao, Chen Wei, Zhang Chuchu, Z U Yaqi","doi":"10.19852/j.cnki.jtcm.20231016.001","DOIUrl":"10.19852/j.cnki.jtcm.20231016.001","url":null,"abstract":"<p><strong>Objective: </strong>To formulate the first clinical practice guideline for the treatment of cough using Chinese medicine based on the grading of recommendations assessment, development, and evaluation (GRADE) systematic approach, including clinical evidence, evaluation of ancient literature, and expert consensus.</p><p><strong>Methods: </strong>In the process of development, the regulation of \"evidence-based, consensus-assisted, and empirical\" was followed, and a comprehensive systematic approach of recommendation assessment, GRADE, evidence-based evaluation, expert consensus, and the Delphi method was used. In the process of guideline development, evidence-based evaluation of ancient literature was included for the first time, and clinical evidence was fully integrated with clinical expert consensus.</p><p><strong>Results: </strong>The clinical practice guidelines for the treatment of cough with Chinese herbal medicine were developed after a comprehensive consideration of evidence-based evaluation and expert opinions. The guideline recommendations focused on recommending herbal compound decoctions and Chinese patent medicines for cough in different conditions. Based on the GRADE systematic approach, we conducted an evidence-based evaluation of the recommended Chinese patent medicines one by one; meanwhile, the expert consensus method was used to unify the recommendations of both.</p><p><strong>Conclusion: </strong>Based on clinical evidence, ancient literature evaluation, and expert consensus, a clinical practice guideline for Traditional Chinese Medicine (TCM) in the treatment of cough was developed, providing the first current clinical practice guideline for domestic and foreign TCM and Western medicine practitioners, especially respiratory professionals at home and abroad.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emodin suppresses alkali burn-induced corneal inflammation and neovascularization by the vascular endothelial growth factor receptor 2 signaling pathway. 大黄素通过血管内皮生长因子受体2信号通路抑制碱烧伤诱导的角膜炎症和新生血管。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20240203.005
Zheng Xueying, Guo Liang, Lai Siyi, L I Fengyue, Liang Mingli, Liu Wanting, Meng Chun, Liu Guanghui

Objective: To investigate the effects of emodin on alkali burn-induced corneal inflammation and neovascularization.

Methods: The ability of emodin to target vascular endothelial growth factor receptor 2 (VEGFR2) was predicted by molecular docking. The effects of emodin on the invasion, migration, and proliferation of human umbilical vein endothelial cells (HUVEC) were determined by cell counting kit-8, Transwell, and tube formation assays. Analysis of apoptosis was performed by flow cytometry. CD31 levels were examined by immunofluorescence. The abundance and phosphorylation state of VEGFR2, protein kinase B (Akt), signal transducer and activator of transcription 3 (STAT3), and P38 were examined by immunoblot analysis. Corneal alkali burn was performed on 40 mice. Animals were divided randomly into two groups, and the alkali-burned eyes were then treated with drops of either 10 μM emodin or phosphate buffered saline (PBS) four times a day. Slit-lamp microscopy was used to evaluate inflammation and corneal neovascularization (CNV) in all eyes on Days 0, 7, 10, and 14. The mice were killed humanely 14 d after the alkali burn, and their corneas were removed and preserved at -80 ℃ until histological study or protein extraction.

Results: Molecular docking confirmed that emodin was able to target VEGFR2. The findings revealed that emodin decreased the invasion, migration, angiogenesis, and proliferation of HUVEC in a dose-dependent manner. In mice, emodin suppressed corneal inflammatory cell infiltration and inhibited the development of corneal neovascularization induced by alkali burn. Compared to those of the PBS-treated group, lower VEGFR2 expression and CD31 levels were found in the emodin-treated group. Emodin dramatically decreased the expression of VEGFR2, p-VEGFR2, p-Akt, p-STAT3, and p-P38 in VEGF-treated HUVEC.

Conclusion: This study provides a new avenue for evaluating the molecular mechanisms underlying corneal inflammation and neovascularization. Emodin might be a promising new therapeutic option for corneal alkali burns.

目的:研究大黄素对碱灼伤引起的角膜炎症和新生血管的影响:研究大黄素对碱烧伤诱导的角膜炎症和新生血管的影响:方法:通过分子对接预测大黄素靶向血管内皮生长因子受体2(VEGFR2)的能力。大黄素对人脐静脉内皮细胞(HUVEC)侵袭、迁移和增殖的影响是通过细胞计数试剂盒-8、Transwell和管形成试验测定的。细胞凋亡分析采用流式细胞术进行。用免疫荧光法检测 CD31 水平。通过免疫印迹分析检测血管内皮生长因子受体2(VEGFR2)、蛋白激酶B(Akt)、信号转导及转录激活因子3(STAT3)和P38的丰度和磷酸化状态。对 40 只小鼠进行角膜碱烧伤。将小鼠随机分为两组,然后用 10 μM 大黄素或磷酸盐缓冲盐水(PBS)滴眼液治疗碱烧伤的眼睛,每天四次。在第 0、7、10 和 14 天,用裂隙灯显微镜评估所有眼睛的炎症和角膜新生血管(CNV)情况。碱烧伤14天后,小鼠被人道处死,角膜被取出并保存在-80 ℃下,直至组织学研究或蛋白质提取:结果:分子对接证实大黄素能靶向血管内皮生长因子受体2。结果:分子对接证实大黄素能靶向血管内皮生长因子受体 2(VEGFR2),并发现大黄素能以剂量依赖的方式减少 HUVEC 的侵袭、迁移、血管生成和增殖。在小鼠中,大黄素能抑制角膜炎症细胞浸润,抑制碱烧伤诱导的角膜新生血管的发展。与 PBS 处理组相比,大黄素处理组的 VEGFR2 表达和 CD31 水平较低。大黄素显著降低了VEGF处理的HUVEC中VEGFR2、p-VEGFR2、p-Akt、p-STAT3和p-P38的表达:这项研究为评估角膜炎症和新生血管形成的分子机制提供了一条新途径。大黄素可能是治疗角膜碱烧伤的一种很有前景的新疗法。
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引用次数: 0
Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang () based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics. 基于高效液相色谱-加热电子喷雾离子化-高分辨质谱和细胞代谢组学的广藿香非挥发性成分抗炎机理研究。
Pub Date : 2024-04-01 DOI: 10.19852/j.cnki.jtcm.20240203.003
Jing Wenguang, Lin Xiaoyu, L I Chu, Zhao Xiaoliang, Cheng Xianlong, Wang Penglong, Wei Feng, M A Shuangcheng

Objective: To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.

Methods: High performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope (HPLC-HESI-HRMS) was used to analyze the chemical constituents of the non-volatile ingredients of patchouli. The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide (LPS) induced RAW264.7 cell inflammation model, and the anti-inflammatory mechanism was investigated using multivariate statistical analysis of cell metabolomics.

Results: The non-volatile ingredients of patchouli were characterized by HPLC-HESI-HRMS, and 36 flavonoids and 18 other components were identified. These ingredients of patchouli not only had a good protective effect on the LPS-induced inflammation model of RAW264.7 cells, but also regulated the expression levels of arginine, L-leucine, cholesterol, fructose and sorbitol by down-regulating arginine metabolism, aminoacyl-tRNA biosynthesis, polyol/sorbitol pathway, so as to reduce inflammation and reduce cell damage.

Conclusion: The non-volatile ingredients of patchouli had good anti-inflammatory effect and exerted its curative effect by regulating endogenous metabolic pathway to reduce inflammatory response.

目的:探讨广藿香中的非挥发性成分的抗炎成分及其作用机制:方法:采用高效液相色谱-加热电子喷雾离子化-高分辨质谱(HPLC-HESI-HRMS)分析广藿香非挥发性成分的化学成分。利用脂多糖(LPS)诱导的 RAW264.7 细胞炎症模型评价了藿香正气水成分的抗炎活性,并利用细胞代谢组学的多元统计分析研究了藿香正气水成分的抗炎机制:结果:采用 HPLC-HESI-HRMS 对广藿香的非挥发性成分进行了表征,鉴定出 36 种黄酮类化合物和 18 种其他成分。藿香正气水中的这些成分不仅对LPS诱导的RAW264.7细胞炎症模型有良好的保护作用,还能通过下调精氨酸代谢、氨基酰-tRNA生物合成、多元醇/山梨醇途径,调节精氨酸、L-亮氨酸、胆固醇、果糖和山梨醇的表达水平,从而减轻炎症反应,减少细胞损伤:结论:藿香正气水中的非挥发性成分具有良好的抗炎作用,通过调节内源性代谢途径来减轻炎症反应,从而发挥治疗作用。
{"title":"Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang () based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics.","authors":"Jing Wenguang, Lin Xiaoyu, L I Chu, Zhao Xiaoliang, Cheng Xianlong, Wang Penglong, Wei Feng, M A Shuangcheng","doi":"10.19852/j.cnki.jtcm.20240203.003","DOIUrl":"10.19852/j.cnki.jtcm.20240203.003","url":null,"abstract":"<p><strong>Objective: </strong>To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.</p><p><strong>Methods: </strong>High performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope (HPLC-HESI-HRMS) was used to analyze the chemical constituents of the non-volatile ingredients of patchouli. The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide (LPS) induced RAW264.7 cell inflammation model, and the anti-inflammatory mechanism was investigated using multivariate statistical analysis of cell metabolomics.</p><p><strong>Results: </strong>The non-volatile ingredients of patchouli were characterized by HPLC-HESI-HRMS, and 36 flavonoids and 18 other components were identified. These ingredients of patchouli not only had a good protective effect on the LPS-induced inflammation model of RAW264.7 cells, but also regulated the expression levels of arginine, L-leucine, cholesterol, fructose and sorbitol by down-regulating arginine metabolism, aminoacyl-tRNA biosynthesis, polyol/sorbitol pathway, so as to reduce inflammation and reduce cell damage.</p><p><strong>Conclusion: </strong>The non-volatile ingredients of patchouli had good anti-inflammatory effect and exerted its curative effect by regulating endogenous metabolic pathway to reduce inflammatory response.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
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