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Regulatory role of electroacupuncture on satellite glial cell activity in the colon and dorsal root ganglion of rats with irritable bowel syndrome. 电针对肠易激综合征大鼠结肠和背根神经节卫星神经胶质细胞活性的调节作用
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.2024.05.005
Zhang Fang, Yan Cuina, Weng Zhijun, W U Luyi, Q I Li, Zhao Min, Xin Yuhu, W U Huangan, Liu Huirong

Objective: To investigate the role of satellite glial cells in irritable bowel syndrome (IBS) and the effect of electroacupuncture (EA) at the Tianshu (ST25) and Shangjuxu (ST37) combination.

Methods: A model for visceral hypersensitivity in IBS was induced through colorectal distension (CRD) stimulation. Clean-grade male Sprague-Dawley (SD) rats were randomly divided into four groups: a normal group (NG), a model group (MG), an electroacupuncture group (EA), and a glial cell inhibitor group (FCA). Bilateral EA (2/100 Hz, 1 mA, 30 min) was administered at the Tianshu (ST25) and Shangjuxu (ST37) in week 6. Abdominal withdrawal reflex (AWR) scores were used to assess the behavioral response associated with visceral hyperalgesia, while hematoxylin-eosin staining was employed to evaluate pathological changes in the colon. The protein and mRNA levels of glial fibrillary acidic protein (GFAP) in the colon and colon-related dorsal root ganglion (DRG) were analyzed using immun-ofluorescence, immun-ohistochemistry, Western blotting, real-time polymerase chain reaction. The impact of EA on electrophysiological properties of colon-related DRG neurons was observed through whole-cell patch clamp analysis.

Results: EA significantly reduced the visceral pain behavior scores in rats with IBS in response to graded (20, 40, 60, 80 mm Hg) CRD stimulation. Additionally, EA downregulated the protein and mRNA expression levels of GFAP in the colon and colon-related DRG of rats with IBS. EA also regulated the resting membrane potential, rheobase and action potential of colon-related DRG neurons in rats with IBS.

Conclusions: EA can regulate the excitatory properties of colon-related DRG neurons by downregulating the protein and mRNA expression of GFAP in the colon and colon-related DRG, indicating a potential neurobiological mechanism by which EA relieves visceral hypersensitivity in rats with IBS.

目的研究卫星神经胶质细胞在肠易激综合征(IBS)中的作用以及电针天枢(ST25)和上巨虚(ST37)的效果:方法:通过刺激结肠直肠胀气(CRD)诱导 IBS 内脏超敏模型。将清洁级雄性 Sprague-Dawley (SD) 大鼠随机分为四组:正常组 (NG)、模型组 (MG)、电针组 (EA) 和神经胶质细胞抑制剂组 (FCA)。第 6 周在天枢(ST25)和上巨虚(ST37)进行双侧 EA(2/100 Hz,1 mA,30 分钟)治疗。腹部退缩反射(AWR)评分用于评估与内脏痛觉减退相关的行为反应,苏木精-伊红染色用于评估结肠的病理变化。使用免疫荧光、免疫组织化学、Western印迹和实时聚合酶链反应分析了结肠和结肠相关背根神经节(DRG)中神经胶质纤维酸性蛋白(GFAP)的蛋白和mRNA水平。通过全细胞膜片钳分析观察了EA对结肠相关DRG神经元电生理特性的影响:结果:EA能明显降低肠易激综合征大鼠在分级(20、40、60、80 mm Hg)CRD刺激下的内脏疼痛行为评分。此外,EA 还下调了肠易激综合征大鼠结肠和结肠相关 DRG 中 GFAP 蛋白和 mRNA 的表达水平。EA还能调节肠易激综合征大鼠结肠相关DRG神经元的静息膜电位、流变基和动作电位:结论:EA能通过下调结肠和结肠相关DRG中GFAP蛋白和mRNA的表达来调节结肠相关DRG神经元的兴奋性,这表明EA能缓解肠易激综合征大鼠内脏超敏反应的潜在神经生物学机制。
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引用次数: 0
Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome. 收费样受体 4/变异髓系分化初级反应 88/核因子卡巴-B 介导的炎症在糖尿病伴西北干燥综合征中的作用。
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.2024.05.004
Deng Deqiang, Xiao Yan, M A Dan, Qiu Jinling, Hao Congli, Wang Di, Zhang Miao

Objective: To investigate the role of toll-like receptor 4 (TLR4)/mutant myeloid differentiation primary response 88 (MyD88)/nuclear factor kappa-B (NF-κB) signaling pathway-mediated inflammation in diabetes mellitus with Northwest dryness syndrome.

Methods: Rats were randomly divided into the normal control, type 2 diabetes (T2DM) model, Northwest dryness syndrome + T2DM (Northwest dryness), and simple internal dampness + T2DM (internal dampness) groups. Enzyme-linked immunosorbent assay was used to detect biochemical indexes and inflammatory factors. The histopathological observation was performed. Quantitative real-time polymerase chain reaction and Western blot analysis were used to detect the mRNA and protein expression levels, respectively.

Results: Compared with the T2DM group, the glycosylated hemoglobin A1c, insulin, glucose tolerance, the homeostasis model assessment of insulin resistance, tumor necrosis factor-α, interleukin 1β, interleukin 16, malondialdehyde, blood lipid, alanine aminotransferase, and aspartate aminotransferase were significantly elevated in the internal dampness group. Their levels were significantly elevated in the Northwest dryness group than in the T2DM and internal dampness groups. The superoxide dismutase, glutathione peroxidase, liver glycogen, and organ-to-weight ratio were significantly declined in the internal dampness group and the Northwest dryness group than in the T2DM group. However, these levels were elevated in the Northwest dryness group than in the internal dampness group. Moreover, the mRNA expression levels of interferon regulatory factor 5 and NF-κB p65, and the protein expression levels of TLR4, MyD88, and NF-κB were significantly higher in the internal dampness and the Northwest dryness groups than the T2DM group. Additionally, the mRNA and protein levels were significantly higher in the Northwest dryness group than in the internal dampness group.

Conclusion: Northwest dryness syndrome-mediated TLR4/MyD88/NF-κB pathway and chronic inflammation might be associated with the occurrence and development of T2DM.

目的研究收费样受体4(TLR4)/突变髓系分化初级反应88(MyD88)/核因子卡巴-B(NF-κB)信号通路介导的炎症在糖尿病合并西北干燥综合征中的作用:将大鼠随机分为正常对照组、2型糖尿病(T2DM)模型组、西北干燥综合征+T2DM(西北干燥)组和单纯内湿+T2DM(内湿)组。采用酶联免疫吸附试验检测生化指标和炎症因子。进行组织病理学观察。采用实时定量聚合酶链反应和 Western 印迹分析分别检测 mRNA 和蛋白质的表达水平:结果:与 T2DM 组相比,内湿组的糖化血红蛋白 A1c、胰岛素、糖耐量、胰岛素抵抗稳态模型评估、肿瘤坏死因子-α、白细胞介素 1β、白细胞介素 16、丙二醛、血脂、丙氨酸氨基转移酶和天冬氨酸氨基转移酶均显著升高,而东北组则显著升高。与 T2DM 组和体内潮湿组相比,西北干燥组的这些指标明显升高。体内潮湿组和西北干燥组的超氧化物歧化酶、谷胱甘肽过氧化物酶、肝糖原和器官重量比明显低于 T2DM 组。但是,西北干燥组的这些水平高于内湿组。此外,干扰素调节因子 5 和 NF-κB p65 的 mRNA 表达水平,以及 TLR4、MyD88 和 NF-κB 的蛋白表达水平在体内潮湿组和西北干燥组均显著高于 T2DM 组。此外,西北干燥组的 mRNA 和蛋白质水平也明显高于内湿组:西北干燥综合征介导的 TLR4/MyD88/NF-κB 通路和慢性炎症可能与 T2DM 的发生和发展有关。
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引用次数: 0
Protective effect of Zhizi Huangqi Shanzha formula on aflatoxin poisoning in mice and its effect on intestinal flora. 枳实黄芪山楂方对小鼠黄曲霉毒素中毒的保护作用及其对肠道菌群的影响
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.2024.05.003
Sun Chuanbo, X U Guangpei, Jiang Ping, Huang Shipping, Chen Cunwu, H E Yanfei

Objective: To evaluate the protective effect of Zhizi Huangqi Shanzha formula (, ZHSF) on aflatoxin-induced liver injury.

Methods: The protective effect of ZHSF on the aflatoxin-induced liver injury was evaluated by histological observation, blood cell analysis, evaluation of liver function and immunity, and gut microbiota analysis.

Results: ZHSF can significantly up-regulate the percentage of lymphocytes and eosinophils in the blood of Aflatoxin B1-intoxicated mice, down-regulate the levels of serum aspartate aminotransferase, alanine aminotransferase, and malondialdehyde, and recover the liver tissue structure. Aflatoxin poisoning induces a variation of the intestinal flora of mice, and ZHSF may recover the variation of intestinal flora induced by Aflatoxin B1. Cluster analysis showed that the intestinal flora of mice in the intervention group was more similar to that of the control group. Correlation analysis showed that Lachnospiraceae, Desulfovibrio, and Lactobacillus may be the key flora for the pharmacological effects of ZHSF.

Conclusions: ZHSF may protect against aflatoxin-induced liver damage, improve immunity, and inhibit oxidative stress by regulating the composition and relative abundance of intestinal flora, which makes it a promising liver-protective candidate drug.

目的:评估枳实黄芪山楂方对黄曲霉毒素所致肝损伤的保护作用:评估枳实黄芪山楂方(ZHSF)对黄曲霉毒素所致肝损伤的保护作用:方法:通过组织学观察、血细胞分析、肝功能和免疫功能评估以及肠道微生物群分析,评价枳实黄芪山楂方对黄曲霉毒素所致肝损伤的保护作用:结果:ZHSF能明显提高黄曲霉毒素B1中毒小鼠血液中淋巴细胞和嗜酸性粒细胞的比例,降低血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶和丙二醛的水平,恢复肝脏组织结构。黄曲霉毒素中毒会引起小鼠肠道菌群失调,而 ZHSF 可以恢复黄曲霉毒素 B1 引起的肠道菌群失调。聚类分析显示,干预组小鼠的肠道菌群与对照组更为相似。相关分析表明,Lachnospiraceae、Desulfovibrio和Lactobacillus可能是ZHSF药理作用的关键菌群:结论:ZHSF可通过调节肠道菌群的组成和相对丰度来防止黄曲霉毒素引起的肝损伤、提高免疫力和抑制氧化应激,因此是一种很有前景的保肝候选药物。
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引用次数: 0
Association of miR-499 rs3746444, miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes. miR-499 rs3746444、miR-149 rs2292832多态性及其表达水平与幽门螺杆菌相关胃病及中医证候的关系
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.2024.05.009
Liu Qi, Y U Chang, Y E Jintong, Zhang Ling, L I Danyan, Dai Yunkai, Zhang Yunzhan, Luo Qi, Chen Weijing, Pan Huaigeng, L I Ruliu, H U Ling

Objective: To provide an objective experimental basis for the gastric mucosa pathological evolution and the transformation of different Traditional Chinese Medicine (TCM) syndromes in helicobacter pylori (H. pylori)-related gastric diseases (HPGD) patients, based on the combination of TCM syndrome differentiation, molecular biology and histopathology.

Methods: A total of 203 participants were enrolled in this study. The expressions of miR-499/miR-149 and H. pylori infection in the gastric tissues from all participants were detected. The genotyping for miR-499 rs3746444 and miR-149 rs2292832 was performed.

Results: In H. pylori positive subjects, the proportion of precancerous gastric lesions (PGL) in liver-stomach disharmony syndrome (LSDS) group was higher than in spleen Qi deficiency syndrome (SQDS) group (P <0.001); The proportion of gastric cancer (GC) in SQDS group was higher than in spleen-stomach damp-heat syndrome (SSDHS) group and LSDS group (all P <0.001). We also found C allele of miR-149 rs2292832 was linked to lower risk of gastric atrophy [miR-149 rs2292832 C vs T: adjusted odds ratio = 0.207; 95% confidence interval (0.043-0.989); P = 0.048]. Compared with healthy control (HC) group, the expression of miR-499 was significantly increased in GC group, while the expression of miR-149 was significantly decreased in chronic inflammation group, PGL group and GC group (all P < 0.05). Test for trend showed that GC risk was on a rising trend with the increasing expression of miR-499 and decreasing expression of miR-149 (both P for trend < 0.05).

Conclusion: The C allele of miR-149 rs2292832 may be a protective factor for gastric mucosal atrophy. H. pylori may participate in the evolution of benign to malignant gastric mucosa lesions by inducing the overexpression of miR-499 and down regulation of miR-149. In addition, patients with H. pylori infection combined SQDS or LSDS may have higher risk of gastric mucosal malignant lesions.

目的结合中医证候分型、分子生物学和组织病理学,为幽门螺杆菌相关胃病(HPGD)患者胃黏膜病理演变及不同中医证候的转化提供客观的实验依据:方法:本研究共纳入 203 名参与者。方法:本研究共纳入 203 名参与者,检测了所有参与者胃组织中 miR-499/miR-149 的表达和幽门螺杆菌感染情况。对 miR-499 rs3746444 和 miR-149 rs2292832 进行了基因分型:结果:在幽门螺杆菌阳性受试者中,肝胃不和综合征(LSDS)组胃癌前病变(PGL)比例高于脾气虚综合征(SQDS)组(P 0.001);SQDS组胃癌(GC)比例高于脾胃湿热综合征(SSDHS)组和LSDS组(均P 0.001)。我们还发现,miR-149 rs2292832的C等位基因与较低的胃萎缩风险有关[miR-149 rs2292832 C vs T:调整后的几率比=0.207;95%置信区间(0.043-0.989);P=0.048]。与健康对照(HC)组相比,GC 组 miR-499 的表达明显增加,而慢性炎症组、PGL 组和 GC 组 miR-149 的表达明显减少(均为 P 0.05)。趋势检验显示,随着miR-499表达量的增加和miR-149表达量的减少,GC风险呈上升趋势(趋势P均<0.05):结论:miR-149 rs2292832的C等位基因可能是胃黏膜萎缩的保护因素。幽门螺杆菌可能通过诱导 miR-499 的过度表达和下调 miR-149 参与胃黏膜病变从良性到恶性的演变。此外,幽门螺杆菌感染合并 SQDS 或 LSDS 的患者发生胃黏膜恶性病变的风险可能更高。
{"title":"Association of miR-499 rs3746444, miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes.","authors":"Liu Qi, Y U Chang, Y E Jintong, Zhang Ling, L I Danyan, Dai Yunkai, Zhang Yunzhan, Luo Qi, Chen Weijing, Pan Huaigeng, L I Ruliu, H U Ling","doi":"10.19852/j.cnki.jtcm.2024.05.009","DOIUrl":"10.19852/j.cnki.jtcm.2024.05.009","url":null,"abstract":"<p><strong>Objective: </strong>To provide an objective experimental basis for the gastric mucosa pathological evolution and the transformation of different Traditional Chinese Medicine (TCM) syndromes in helicobacter pylori (H. pylori)-related gastric diseases (HPGD) patients, based on the combination of TCM syndrome differentiation, molecular biology and histopathology.</p><p><strong>Methods: </strong>A total of 203 participants were enrolled in this study. The expressions of miR-499/miR-149 and H. pylori infection in the gastric tissues from all participants were detected. The genotyping for miR-499 rs3746444 and miR-149 rs2292832 was performed.</p><p><strong>Results: </strong>In H. pylori positive subjects, the proportion of precancerous gastric lesions (PGL) in liver-stomach disharmony syndrome (LSDS) group was higher than in spleen Qi deficiency syndrome (SQDS) group (<i>P <</i>0.001); The proportion of gastric cancer (GC) in SQDS group was higher than in spleen-stomach damp-heat syndrome (SSDHS) group and LSDS group (all <i>P <</i>0.001). We also found C allele of miR-149 rs2292832 was linked to lower risk of gastric atrophy [miR-149 rs2292832 C <i>vs</i> T: adjusted odds ratio = 0.207; 95% confidence interval (0.043-0.989); <i>P =</i> 0.048]. Compared with healthy control (HC) group, the expression of miR-499 was significantly increased in GC group, while the expression of miR-149 was significantly decreased in chronic inflammation group, PGL group and GC group (all <i>P <</i> 0.05). Test for trend showed that GC risk was on a rising trend with the increasing expression of miR-499 and decreasing expression of miR-149 (both <i>P</i> for trend < 0.05).</p><p><strong>Conclusion: </strong>The C allele of miR-149 rs2292832 may be a protective factor for gastric mucosal atrophy. H. pylori may participate in the evolution of benign to malignant gastric mucosa lesions by inducing the overexpression of miR-499 and down regulation of miR-149. In addition, patients with H. pylori infection combined SQDS or LSDS may have higher risk of gastric mucosal malignant lesions.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of international guidelines by Tuina practitioners for specific acupoints of paediatrics Tuina (2022 version). 由推拿医师制定儿科推拿特定穴位的国际指南(2022 年版)。
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.2024.05.011
Lan Xiaoxue, Sun Yanan, Weng Zhiwen, Wang Yue, Zhang Ying, Liang Yuanwen, G U Sirui, Zhou Rong, Chen Qianji, Jia Baolin, B O Han, Wang Fangying, H E Qiang, Zhang Jie, Tan Jiang, Y E Xingzhu, Wang Xiyou, Y U Changhe, Chen Hong

Objective: To establish a standardized framework encompassing the precise locations, manipulations, functions and indications of specific acupoints in the field of paediatric Tuina.

Methods: The development of consensus involved three distinct stages. Initially, a list of paediatric Tuina specific acupoints was compiled based on an extensive literature review, which was subsequently supplemented through expert interviews. In the second stage, the Delphi method was employed to assess the significance of acupoint locations, manipulations, functions, and indications. In situations where the questionnaire survey failed to yield agreement or when the experts held reservations, the nominal group approach was utilized during the expert consensus meeting. The final version of the technical standardized material was ultimately determined during an expert consensus conference. After undergoing external peer review and evaluation, the completed draft was prepared for public dissemination RESULTS: The comprehensive list identified a total of 66 specific acupoints. The location and manipulation questionnaire consisted of 156 items based on the literature database, while the function and indication questionnaire contained 116 items. Two rounds of Delphi surveys were conducted for the location and manipulation category, and another two rounds of Delphi surveys were conducted for the function and indication category. During the experts consensus meeting The panel of experts conducted in-depth discussions on 61 questions, resulting in the formulation of technical guidelines for the locations, manipulations, functions, and indications of 64 paediatric Tuina acupoints. Subsequently, the research team compiled and edited the draft of the technical guidelines for acupoints of paediatric Tuina, which was finalized after external review and feedback.

Conclusion: This study successfully established the recognized technical standards for practitioners of paediatric Tuina, thereby standardizing clinical practices and providing a foundation setting the framework for future research. The guidelines offer theoretical insights and recommendations for conducting clinical studies comparing different acupoint sites, as well as modifying or enhancing treatment regimens.

目的建立一个标准化框架,涵盖儿科推拿领域特定穴位的精确位置、操作、功能和适应症:达成共识的过程分为三个不同阶段。首先,在广泛查阅文献的基础上编制了一份儿科推拿特定穴位列表,随后通过专家访谈对该列表进行了补充。在第二阶段,采用德尔菲法评估穴位位置、操作、功能和适应症的重要性。在问卷调查未能达成一致或专家持保留意见的情况下,专家共识会议采用了名义小组法。技术标准材料的最终版本在专家共识会议上最终确定。经过外部同行评审和评估后,完成的草案准备向公众发布 结果:综合清单共确定了 66 个具体穴位。根据文献数据库,位置和操作问卷包含 156 个项目,而功能和适应症问卷包含 116 个项目。针对定位和操作类别进行了两轮德尔菲调查,针对功能和适应症类别进行了两轮德尔菲调查。在专家共识会议上 专家小组对 61 个问题进行了深入讨论,最终制定了 64 个小儿推拿穴位的位置、手法、功能和适应症的技术指南。随后,研究小组对《小儿推拿穴位技术指南》草案进行了整理和编辑,经外部评审和反馈后最终定稿:本研究成功地为小儿推拿从业人员建立了公认的技术标准,从而规范了临床实践,为今后的研究提供了基础框架。该指南为开展比较不同穴位部位的临床研究以及修改或改进治疗方案提供了理论见解和建议。
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引用次数: 0
Qingfei Zhisou oral liquid alleviates fever-induced inflammation by regulating arachidonic acid and lysophospholipids metabolism and inhibiting hypothalamus transient receptor potential ion channels expression. 清热解毒口服液通过调节花生四烯酸和溶血磷脂代谢,抑制下丘脑瞬时受体电位离子通道的表达,缓解发热引起的炎症。
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.20240806.003
Gao Jiaming, Zhang Yehao, Chen Yuanyuan, Jin Long, Zhao Jianfeng, Guo Hao, F U Jianhua

Objective: To explore how Qingfei Zhisou oral liquid (, QFZS) adjusts body temperature bias and the interaction of inflammatory factors levels and metabolomic differences.

Methods: Dry yeast was subcutaneously injected at 10 mL/kg to establish the pyrexia model. We randomly divided 60 Sprague-Dawley rats into five groups: control, model, positive, low dose of QFZS and high dose of QFZS. Inflammatory proteins were evaluated by Western blotting and immunohistochemistry. For the examination of the endogenous metabolites, enzyme linked immunosorbent assay and ultra-high-performance liquid chromatography high-resolution mass spectrometry were employed.

Results: QFZS significantly reduced rats' body temperature within 6 h after dry yeast injection and reduced the secretion of the arginine vasopressin, cyclic adenosine monophosphate, prostaglandin E-2, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β in serum. Meanwhile, we identified 41 metabolites between the model and QFZS groups, including arachidonic acid and lysophospholipids. QFZS restored normal arachidonic acid levels. Based on the differential metabolite enrichment analysis, QFZS's anti-inflammatory and anti-pyrexia effects might be related to the inflammatory pathway regulated by transient receptor potential. Additionally, QFZS treatment reduced transient receptor potential melastatin 2 ion channel expression and affected TNF-α, heat shock protein 70, and cyclooxygenase-2 expression in the hypothalamus.

Conclusion: QFZS exerts its regulatory effects on fever by regulating the metabolism of lysophospholipids and arachidonic acid and the regulation of inflammation via transient receptor potential ion channels channels.

目的探讨清热解毒口服液(QFZS)如何调节体温偏差以及炎症因子水平和代谢组差异的相互作用:方法:以 10 mL/kg 的剂量皮下注射干酵母,建立热病模型。我们将 60 只 Sprague-Dawley 大鼠随机分为五组:对照组、模型组、阳性组、低剂量 QFZS 组和高浓度 QFZS 组。用 Western 印迹法和免疫组化法评估炎症蛋白。在检测内源性代谢物时,采用了酶联免疫吸附测定法和超高效液相色谱-高分辨质谱法:结果:干酵母注射后6 h内,QFZS能明显降低大鼠体温,减少血清中精氨酸加压素、环磷酸腺苷、前列腺素E-2、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-1β的分泌。同时,我们在模型组和 QFZS 组之间发现了 41 种代谢物,包括花生四烯酸和溶血磷脂。QFZS恢复了正常的花生四烯酸水平。根据差异代谢物富集分析,QFZS的抗炎和抗厌食作用可能与瞬时受体电位调控的炎症通路有关。此外,QFZS还能降低瞬时受体电位美拉塔素2离子通道的表达,并影响下丘脑中TNF-α、热休克蛋白70和环氧合酶-2的表达:结论:QFZS通过调节溶血磷脂和花生四烯酸的代谢,并通过瞬态受体电位离子通道调节炎症,从而对发热产生调节作用。
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引用次数: 0
Neuroprotective effect of Naochuxue prescription on intracerebral hemorrhage: inhibition of autophagy downregulating high mobility group box-1. 野菊花方对脑出血的神经保护作用:抑制自噬下调高迁移率基团框-1
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.20240515.003
Jin Hong, Wang Xinna, Wang Ruonan, L I Jinjian, Y U Junchao, Zhao Dexi, Zhai Lu

Objective: To determine the molecular mechanisms underlying the neuroprotective effects of Naochuxue prescription (,NCXP) in rats with intracerebral hemorrhage (ICH).

Methods: Sprague-Dawley rats were injected with collagenase to generate ICH models, which were then randomly divided into six groups, including control, sham, model, and three intervention groups. The intervention groups received different doses of NCXP (0.13, 0.26, and 0.52 g/kg) daily for 10 d. High-performance liquid chromatography (HPLC) was used to analyze the chemical characteristics of NCXP. The neurobehavioral outcomes of the rats were evaluated using neurological deficit scores (Zea Longa 5) and the corner turn test. Pathomorphological changes in perihematomal tissues after ICH were observed using hematoxylin and eosin staining. Immunohistochemistry (IHC) was used to detect the inflammation expression of interleukin 6 (IL-6) and toll-like receptor 4 (TLR4). High mobility group box-1 (HMGB1), Beclin1, microtubule-associated protein 1 light chain 3 beta (LC3), and sequestosome 1 (p62) were detected using real-time quantitative polymerase chain reaction and Western blotting in perihematomal tissues.

Results: HPLC showed that the NCXP had good stability. Rats with ICH had severe neurological function deficits compared to the control group. IHC results showed that NCXP significantly downregulated the expression of the inflammatory proteins IL-6 and TLR4. ICH rats treated with NCXP showed less neurological injury than the model group, accompanied by a significantly decreased expression of HMGB1, Beclin1, and LC3 and an increased expression of p62.

Conclusions: The neuroprotective effect of NCXP alleviated inflammation and autophagy possibly by downregulating HMGB1 expression. However, further research on the signaling pathways is required to verify this hypothesis.

目的方法:向Sprague-Dawley大鼠注射胶原酶,制成ICH模型,然后随机分为6组,包括对照组、假组、模型组和3个干预组:方法:给Sprague-Dawley大鼠注射胶原酶以产生ICH模型,然后将其随机分为六组,包括对照组、假组、模型组和三个干预组。采用高效液相色谱法(HPLC)分析 NCXP 的化学特性。使用神经功能缺损评分(Zea Longa 5)和转角测试评估大鼠的神经行为结果。使用苏木精和伊红染色观察 ICH 后血肿周围组织的病理形态学变化。免疫组化(IHC)用于检测白细胞介素6(IL-6)和类收费受体4(TLR4)的炎症表达。采用实时定量聚合酶链反应和 Western 印迹法检测血细胞周围组织中的高迁移率基团盒-1(HMGB1)、Beclin1、微管相关蛋白 1 轻链 3 beta(LC3)和序列体 1(p62):高效液相色谱法显示,NCXP 具有良好的稳定性。与对照组相比,ICH 大鼠有严重的神经功能缺损。IHC 结果显示,NCXP 能显著降低炎症蛋白 IL-6 和 TLR4 的表达。与模型组相比,接受 NCXP 治疗的 ICH 大鼠神经损伤较轻,同时 HMGB1、Beclin1 和 LC3 的表达明显减少,p62 的表达增加:NCXP的神经保护作用可能通过下调HMGB1的表达缓解了炎症和自噬。然而,要验证这一假设,还需要进一步研究信号通路。
{"title":"Neuroprotective effect of Naochuxue prescription on intracerebral hemorrhage: inhibition of autophagy downregulating high mobility group box-1.","authors":"Jin Hong, Wang Xinna, Wang Ruonan, L I Jinjian, Y U Junchao, Zhao Dexi, Zhai Lu","doi":"10.19852/j.cnki.jtcm.20240515.003","DOIUrl":"10.19852/j.cnki.jtcm.20240515.003","url":null,"abstract":"<p><strong>Objective: </strong>To determine the molecular mechanisms underlying the neuroprotective effects of Naochuxue prescription (,NCXP) in rats with intracerebral hemorrhage (ICH).</p><p><strong>Methods: </strong>Sprague-Dawley rats were injected with collagenase to generate ICH models, which were then randomly divided into six groups, including control, sham, model, and three intervention groups. The intervention groups received different doses of NCXP (0.13, 0.26, and 0.52 g/kg) daily for 10 d. High-performance liquid chromatography (HPLC) was used to analyze the chemical characteristics of NCXP. The neurobehavioral outcomes of the rats were evaluated using neurological deficit scores (Zea Longa 5) and the corner turn test. Pathomorphological changes in perihematomal tissues after ICH were observed using hematoxylin and eosin staining. Immunohistochemistry (IHC) was used to detect the inflammation expression of interleukin 6 (IL-6) and toll-like receptor 4 (TLR4). High mobility group box-1 (HMGB1), Beclin1, microtubule-associated protein 1 light chain 3 beta (LC3), and sequestosome 1 (p62) were detected using real-time quantitative polymerase chain reaction and Western blotting in perihematomal tissues.</p><p><strong>Results: </strong>HPLC showed that the NCXP had good stability. Rats with ICH had severe neurological function deficits compared to the control group. IHC results showed that NCXP significantly downregulated the expression of the inflammatory proteins IL-6 and TLR4. ICH rats treated with NCXP showed less neurological injury than the model group, accompanied by a significantly decreased expression of HMGB1, Beclin1, and LC3 and an increased expression of p62.</p><p><strong>Conclusions: </strong>The neuroprotective effect of NCXP alleviated inflammation and autophagy possibly by downregulating HMGB1 expression. However, further research on the signaling pathways is required to verify this hypothesis.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuro- and immuno-modulation mediated by the cardiac sympathetic nerve: a novel insight into the anti-ischemic efficacy of acupuncture. 由心脏交感神经介导的神经和免疫调节:针灸抗缺血疗效的新见解。
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.20240423.001
X I Hanqing, L I Xia, Zhang Ziyi, Cui Xiang, Jing Xianghong, Zhu Bing, Gao Xinyan

Communication between sympathetic nerves and the immune system is a crucial and active process during myocardial ischemia (MI), as myocardial damage and inflammatory stimuli concurrently occur. Sympathetic nerves undergo structural and functional changes after MI, leading to adverse left ventricular (LV) remodeling and heart failure (HF). The complex inflammatory response to MI, including local myocardial anti-inflammatory repair and systemic immune reactions, plays a key role in adverse LV remodeling. Here, we review the progressive structural and electrophysiological remodeling of the LV and the involvement of sympathetic tone in complex and dynamic processes that are susceptible to MI pathological conditions. Acupuncture has been reported to effectively improve cardiac function, eliminate arrhythmia, and mitigate adverse LV remodeling via somatosensory regulation after MI. Moreover, acupuncture has an anti-inflammatory effect on the pathological process of myocardial ischemia. In this Review, we aim to summarize the involvement of sympathetic nerve activation in the neuro-immune modulation of structural and functional cardiac changes after MI. As a noninvasive method for sympathetic regulation, acupuncture is an ideal option because of its anti-ischemic efficacy. A better understanding of the neural circuitry that regulates cardiac function and immune responses following MI could reveal novel targets for acupuncture treatment.

在心肌缺血(MI)期间,由于心肌损伤和炎症刺激同时发生,交感神经和免疫系统之间的交流是一个关键而活跃的过程。心肌缺血后交感神经的结构和功能会发生变化,导致左心室重塑和心力衰竭。心肌梗死后复杂的炎症反应,包括局部心肌抗炎修复和全身免疫反应,在左心室不良重塑中起着关键作用。在此,我们回顾了左心室进行性结构和电生理重塑,以及交感神经张力参与易受心肌缺血病理条件影响的复杂和动态过程。据报道,针灸可有效改善心功能,消除心律失常,并通过体感调节减轻心肌梗死后左心室的不良重塑。此外,针灸对心肌缺血的病理过程具有抗炎作用。在本综述中,我们旨在总结交感神经激活参与心肌梗死后心脏结构和功能变化的神经免疫调节。作为一种非侵入性的交感神经调节方法,针灸因其抗缺血的功效而成为一种理想的选择。如果能更好地了解调节心肌梗死后心脏功能和免疫反应的神经回路,就能发现针灸治疗的新靶点。
{"title":"Neuro- and immuno-modulation mediated by the cardiac sympathetic nerve: a novel insight into the anti-ischemic efficacy of acupuncture.","authors":"X I Hanqing, L I Xia, Zhang Ziyi, Cui Xiang, Jing Xianghong, Zhu Bing, Gao Xinyan","doi":"10.19852/j.cnki.jtcm.20240423.001","DOIUrl":"https://doi.org/10.19852/j.cnki.jtcm.20240423.001","url":null,"abstract":"<p><p>Communication between sympathetic nerves and the immune system is a crucial and active process during myocardial ischemia (MI), as myocardial damage and inflammatory stimuli concurrently occur. Sympathetic nerves undergo structural and functional changes after MI, leading to adverse left ventricular (LV) remodeling and heart failure (HF). The complex inflammatory response to MI, including local myocardial anti-inflammatory repair and systemic immune reactions, plays a key role in adverse LV remodeling. Here, we review the progressive structural and electrophysiological remodeling of the LV and the involvement of sympathetic tone in complex and dynamic processes that are susceptible to MI pathological conditions. Acupuncture has been reported to effectively improve cardiac function, eliminate arrhythmia, and mitigate adverse LV remodeling <i>via</i> somatosensory regulation after MI. Moreover, acupuncture has an anti-inflammatory effect on the pathological process of myocardial ischemia. In this Review, we aim to summarize the involvement of sympathetic nerve activation in the neuro-immune modulation of structural and functional cardiac changes after MI. As a noninvasive method for sympathetic regulation, acupuncture is an ideal option because of its anti-ischemic efficacy. A better understanding of the neural circuitry that regulates cardiac function and immune responses following MI could reveal novel targets for acupuncture treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Traditional Chinese Medicine in the treatment of recurrent respiratory tract infections in children: an overview of systematic reviews and Meta-analyses. 中医药治疗儿童反复呼吸道感染:系统综述和 Meta 分析概述。
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.20240806.007
Deng Yasheng, Han Siyin, X I Lanhua, Huang Hui, Liang Tianwei, Zheng Yiqing, Fan Yanping, Lin Jiang

Objective: To evaluate and summarise the evidence from published Meta-analyses/systematic reviews (MAs/SRs) of Traditional Chinese Medicine (TCM) in the treatment of recurrent respiratory tract infections (RRTIs) and to provide a scientific basis for the clinical treatment of RRTIs with TCM.

Methods: Studies were retrieved from Chinese and English databases including the China National Knowledge Infrastructure, Wanfang database, China Science and Technology Journal Database, SinoMed, PubMed, Web of Science, the Cochrane Library and EMbase from their establishment date to March 2023. Involved studies were screened, extracted, and evaluated for quality by two researchers independently. The a measurement tool to assess systematic reviews (AMSTAR) 2 scale was used for methodological quality evaluation, as well as the preferred reporting items for systematic reviews and Meta-analyses (PRISMA) 2020 statement for report quality evaluation, the risk of bias in systematic reviews (ROBIS) tool for risk of bias, and the grading of recommendations, assessment, development and evaluation (GRADE) quality assessment tool for evidence quality.

Results: Twenty MAs/SRs studies were included, including analyses of 274 original studies involving 38 335 patients with RRTIs. The AMSTAR 2 scale evaluation results showed that 19 studies were of very low quality and one of moderate quality. The ROBIS evaluation results showed that 11 MAs/SRs were at high risk and nine at low risk of bias. The PRISMA 2020 report quality showed the included studies had scores between 23.5 and 35.5, among them one with high quality, 17 with moderate quality and two with low quality. The GRADE system results showed that among 126 outcome indicators, only 17 had moderate quality of evidence, 27 had low quality, 82 had very low quality, and none had high quality.

Conclusions: The MAs/SRs methodological quality of using TCM for treatment RRTIs is generally poor, the quality of reports as well as of evidence is generally low, and the risk of bias is high; therefore we should treat these results with caution.

目的评估和总结已发表的中医药治疗反复呼吸道感染(RRTIs)的Meta分析/系统综述(MAs/SRs)证据,为中医药临床治疗RRTIs提供科学依据:方法:从中国知网、万方数据库、中国科技期刊数据库、SinoMed、PubMed、Web of Science、Cochrane Library和EMbase等中英文数据库中检索自建立之日起至2023年3月的相关研究。所涉及的研究由两名研究人员独立进行筛选、提取和质量评估。方法学质量评估采用系统综述评估工具(AMSTAR)2量表,报告质量评估采用系统综述和元分析首选报告项目(PRISMA)2020声明,偏倚风险采用系统综述偏倚风险工具(ROBIS),证据质量采用建议、评估、发展和评价分级(GRADE)质量评估工具:共纳入 20 项 MAs/SRs 研究,包括对 274 项原始研究的分析,涉及 38 335 名 RRTI 患者。AMSTAR 2量表评估结果显示,19项研究的质量很低,1项为中等质量。ROBIS 评估结果显示,11 项 MAs/SR 存在高偏倚风险,9 项存在低偏倚风险。PRISMA 2020 报告质量显示,纳入研究的得分介于 23.5 和 35.5 之间,其中 1 项为高质量,17 项为中等质量,2 项为低质量。GRADE 系统结果显示,在 126 项结果指标中,只有 17 项具有中等证据质量,27 项质量较低,82 项质量很低,没有一项具有高质量:结论:使用中医药治疗 RRTIs 的 MAs/SRs 方法学质量普遍较差,报告和证据的质量普遍较低,偏倚风险较高;因此,我们应谨慎对待这些结果。
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引用次数: 0
Relative exchangeable copper, a high-quality biomarker for differentiation of Traditional Chinese Medicine syndrome in Wilson's disease. 相对可交换铜--区分威尔森氏病中医综合征的优质生物标志物
Pub Date : 2024-10-01 DOI: 10.19852/j.cnki.jtcm.20240828.007
Ding Yufeng, Yang Wenming, Cheng Yuan, Yang Yulong, Zhu Jun, L U Yachun, Fang Xiang, Zhang Jing

Objective: To investigate the value of relative exchangeable copper (REC) in diagnosing Wilson's disease (WD) and to determine its significance in the differentiation of Traditional Chinese Medicine (TCM) syndrome.

Methods: A total of 78 patients with WD were recruited on the same day of the medical visit, and among them, 32 were suffering from non-WD (N-WD) and 37 were heterozygous ATP7B carriers (HC) enrolled as controls. Molecular genetic testing was performed for diagnosing WD and HC. Additionally, REC levels in different TCM syndromes were investigated. The correlation between REC and serum ceruloplasmin (Cp), serum copper oxidase (CO), and 24-h urinary copper was analyzed using the Global Assessment Scale and Unified Wilson's Disease Rating Scale and the significance of REC in WD diagnosis was investigated. Resting-state functional magnetic resonance imaging was used to assess the clinical symptoms of WD and analyze its severity in different TCM syndromes.

Results: REC determination helped in significantly distinguishing patients with N-WD or HC from those with WD with a cut-off of 21.15%. Furthermore, the comparative analysis of REC ratios among different TCM syndromes showed markedly high REC levels in the dampness-heat internal accumulation syndrome group. Additionally, the seed-based functional connectivity value from the putamen to the cerebellum was significantly correlated with TCM syndromes.

Conclusion: REC level is a high-quality biomarker for diagnosing WD that effectively reflects disease severity and plays an essential role in deciding on treatment strategies and prognosis. Furthermore, REC levels are closely related to TCM syndromes in WD; thus, it is a potential objective quantitative indicator for distinguishing TCM syndromes in WD.

目的研究相对可交换铜(REC)在诊断威尔逊氏病(WD)中的价值,并确定其在中医证候鉴别中的意义:方法:在就诊当日共招募 78 名威尔森氏病患者,其中 32 名为非威尔森氏病患者(N-WD),37 名为杂合子 ATP7B 携带者(HC)作为对照。对 WD 和 HC 的诊断进行了分子基因检测。此外,还调查了不同中医综合征中的 REC 水平。使用全球评估量表和统一威尔逊氏病评分量表分析了REC与血清脑磷脂(Cp)、血清铜氧化酶(CO)和24小时尿铜之间的相关性,并研究了REC在WD诊断中的意义。静息态功能磁共振成像用于评估WD的临床症状,并分析其在不同中医综合征中的严重程度:结果:REC测定有助于明显区分N-WD或HC患者与WD患者,其临界值为21.15%。此外,不同中医综合征的 REC 比率比较分析显示,湿热内蕴证组的 REC 水平明显较高。此外,基于种子选手的功能连接值(从推拿门到小脑)与中医综合征显著相关:结论:REC水平是诊断WD的高质量生物标志物,能有效反映疾病的严重程度,并在决定治疗策略和预后方面发挥重要作用。此外,REC水平与WD的中医综合征密切相关,因此,它是区分WD中医综合征的一个潜在的客观量化指标。
{"title":"Relative exchangeable copper, a high-quality biomarker for differentiation of Traditional Chinese Medicine syndrome in Wilson's disease.","authors":"Ding Yufeng, Yang Wenming, Cheng Yuan, Yang Yulong, Zhu Jun, L U Yachun, Fang Xiang, Zhang Jing","doi":"10.19852/j.cnki.jtcm.20240828.007","DOIUrl":"10.19852/j.cnki.jtcm.20240828.007","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the value of relative exchangeable copper (REC) in diagnosing Wilson's disease (WD) and to determine its significance in the differentiation of Traditional Chinese Medicine (TCM) syndrome.</p><p><strong>Methods: </strong>A total of 78 patients with WD were recruited on the same day of the medical visit, and among them, 32 were suffering from non-WD (N-WD) and 37 were heterozygous ATP7B carriers (HC) enrolled as controls. Molecular genetic testing was performed for diagnosing WD and HC. Additionally, REC levels in different TCM syndromes were investigated. The correlation between REC and serum ceruloplasmin (Cp), serum copper oxidase (CO), and 24-h urinary copper was analyzed using the Global Assessment Scale and Unified Wilson's Disease Rating Scale and the significance of REC in WD diagnosis was investigated. Resting-state functional magnetic resonance imaging was used to assess the clinical symptoms of WD and analyze its severity in different TCM syndromes.</p><p><strong>Results: </strong>REC determination helped in significantly distinguishing patients with N-WD or HC from those with WD with a cut-off of 21.15%. Furthermore, the comparative analysis of REC ratios among different TCM syndromes showed markedly high REC levels in the dampness-heat internal accumulation syndrome group. Additionally, the seed-based functional connectivity value from the putamen to the cerebellum was significantly correlated with TCM syndromes.</p><p><strong>Conclusion: </strong>REC level is a high-quality biomarker for diagnosing WD that effectively reflects disease severity and plays an essential role in deciding on treatment strategies and prognosis. Furthermore, REC levels are closely related to TCM syndromes in WD; thus, it is a potential objective quantitative indicator for distinguishing TCM syndromes in WD.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
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