Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90916-8
D.R. Threlfall, T.W. Goodwin
{"title":"Ubiquinone-50 and plastoquinone-45 in plant tissue cultures of Paul's scarlet rose","authors":"D.R. Threlfall, T.W. Goodwin","doi":"10.1016/0006-3002(63)90916-8","DOIUrl":"10.1016/0006-3002(63)90916-8","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 532-534"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90916-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90929-6
B. Blombäck , M. Blombäck , R.F. Doolittle , B. Hessel , P. Edman
{"title":"On the properties of a new human fibrinopeptide","authors":"B. Blombäck , M. Blombäck , R.F. Doolittle , B. Hessel , P. Edman","doi":"10.1016/0006-3002(63)90929-6","DOIUrl":"10.1016/0006-3002(63)90929-6","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 563-566"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90929-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90911-9
J. Jadot, J. Casimir, F. Alderweireldt
l-threo-β-Hydroxyleucine has been isolated from Deutzia gracilis by ion-exchange resin chromatography, and by preparative paper chromatography. The substance is reduced to leucine by hydroiodic acid and offers the characteristic reaction of β-hydroxyamino acids with periodic acid. Alkaline decomposition confirms the β position of the alcohol function. The natural substance and the dl-threo-β-hydroxyleucine are inseparable by paper chromatography, and the study of their nuclear-magnetic-resonance spectra brings supplementary arguments in favour of the threo form. The natural substance has the same infrared spectrum as synthetic threo-β-hydroxyleucine.
{"title":"Caractérisation de la l-thréo-β-hydroxyleucine à partir de Deutzia gracilis","authors":"J. Jadot, J. Casimir, F. Alderweireldt","doi":"10.1016/0006-3002(63)90911-9","DOIUrl":"10.1016/0006-3002(63)90911-9","url":null,"abstract":"<div><p><span>l</span>-<em>threo</em>-β-Hydroxyleucine has been isolated from <em>Deutzia gracilis</em> by ion-exchange resin chromatography, and by preparative paper chromatography. The substance is reduced to leucine by hydroiodic acid and offers the characteristic reaction of β-hydroxyamino acids with periodic acid. Alkaline decomposition confirms the β position of the alcohol function. The natural substance and the <span>dl</span>-<em>threo</em>-β-hydroxyleucine are inseparable by paper chromatography, and the study of their nuclear-magnetic-resonance spectra brings supplementary arguments in favour of the <em>threo</em> form. The natural substance has the same infrared spectrum as synthetic <em>threo</em>-β-hydroxyleucine.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 500-504"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90911-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80817816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90906-5
Kristen B. Eik-nes, Madhukar Kekre
The formation of testosterone in vivo has been investigated in anesthetized dogs by infusing radioactive steroids via the left spermatic artery and isolating radioactive testosterone in venous blood obtained from the left spermatic vein. The data from such experiments indicate that the formation of 17α-hydroxypregnenolone and the metabolism of this steroid to testosterone is a major route in the biosynthesis in vivo of testosterone from pregnenolone by the canine testis.
1.
1. When an equal amount of [4−14C]progesterone and 17α-hydroxy[7α-3H]pregnenolone were infused at a constant rate by the spermatic artery, the concentration of 3H in isolated spermatic vein testosterone was from 3–8 times that of 14C.
2.
2. When [7α-3H]prenenolone was infused by the left spermatic artery for 5 min, more [3H]17α-hydroxypregnenolone than [3H]progesterone was found in the left testis at the end of this infusion.
3.
3. The problem of preferred metabolism to testosterone in vivo by testicular tissue is discussed.
{"title":"Metabolism in vivo of steroids by the canine testes","authors":"Kristen B. Eik-nes, Madhukar Kekre","doi":"10.1016/0006-3002(63)90906-5","DOIUrl":"10.1016/0006-3002(63)90906-5","url":null,"abstract":"<div><p>The formation of testosterone <em>in vivo</em> has been investigated in anesthetized dogs by infusing radioactive steroids <em>via</em> the left spermatic artery and isolating radioactive testosterone in venous blood obtained from the left spermatic vein. The data from such experiments indicate that the formation of 17α-hydroxypregnenolone and the metabolism of this steroid to testosterone is a major route in the biosynthesis <em>in vivo</em> of testosterone from pregnenolone by the canine testis. </p><ul><li><span>1.</span><span><p>1. When an equal amount of [4<sup>−14</sup>C]progesterone and 17α-hydroxy[7α-<sup>3</sup>H]pregnenolone were infused at a constant rate by the spermatic artery, the concentration of <sup>3</sup>H in isolated spermatic vein testosterone was from 3–8 times that of <sup>14</sup>C.</p></span></li><li><span>2.</span><span><p>2. When [7α-<sup>3</sup>H]prenenolone was infused by the left spermatic artery for 5 min, more [<sup>3</sup>H]17α-hydroxypregnenolone than [<sup>3</sup>H]progesterone was found in the left testis at the end of this infusion.</p></span></li><li><span>3.</span><span><p>3. The problem of preferred metabolism to testosterone <em>in vivo</em> by testicular tissue is discussed.</p></span></li></ul></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 449-456"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90906-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23670744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90930-2
Ralph G. Wieland, Richard P. Levy, David Katz, H. Hirschmann
{"title":"Evidence for secretion of 3β-hydroxyandrost-5-en-17-one sulfate by measurement in normal human adrenal venous blood","authors":"Ralph G. Wieland, Richard P. Levy, David Katz, H. Hirschmann","doi":"10.1016/0006-3002(63)90930-2","DOIUrl":"10.1016/0006-3002(63)90930-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 566-568"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90930-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90920-X
Grace L. Rosenquist , R.V. Gilden
{"title":"Chicken antibodies to bovine serum albumin molecular size and sensitivity to 2-mercaptoethanol","authors":"Grace L. Rosenquist , R.V. Gilden","doi":"10.1016/0006-3002(63)90920-X","DOIUrl":"10.1016/0006-3002(63)90920-X","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 543-545"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90920-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90925-9
T.N.R. Varma , T.K. Murray
{"title":"Conversion of retro-vitamin A to vitamin A in the vitamin A-deficient rat","authors":"T.N.R. Varma , T.K. Murray","doi":"10.1016/0006-3002(63)90925-9","DOIUrl":"10.1016/0006-3002(63)90925-9","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 556-558"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90925-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90926-0
Tairo Oshima , Sadaaki Kawai , Fujio Egami
{"title":"Incorporation of oxygen atoms from atmospheric oxygen into indigotin by Pseudomonas indoloxidans","authors":"Tairo Oshima , Sadaaki Kawai , Fujio Egami","doi":"10.1016/0006-3002(63)90926-0","DOIUrl":"10.1016/0006-3002(63)90926-0","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 558-559"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90926-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-11-15DOI: 10.1016/0006-3002(63)90927-2
B. Keil, Z. Prusík, F. Šorm
{"title":"Disulphide bridges and a suggested structure of chymotrypsinogen","authors":"B. Keil, Z. Prusík, F. Šorm","doi":"10.1016/0006-3002(63)90927-2","DOIUrl":"10.1016/0006-3002(63)90927-2","url":null,"abstract":"","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 3","pages":"Pages 559-561"},"PeriodicalIF":0.0,"publicationDate":"1963-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)90927-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23672502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1963-10-29DOI: 10.1016/0006-3002(63)91634-2
Eiji Ito, Masahiro Saito
Under incubation conditions including deprivation of nitrogen sources, deprivation of lysine, inhibition by oxamycin and inhibition by penicillin, the accumulation of UDP-N-acetylamino sugar derivatives in Staphylococcus aureus cells, with time, has been studied. UDP-N-acetylglucosamine and UDP-N-acetylglucosamine-3-O-lactic ether accumulated in cells which were incubated in amino acid-free medium. During the initial phase of incubation there was a rapid rise in the appearance of UDP-N-acetylglucosamine followed later by a decrease of this nucleotide; concomitant accumulation of UDP-N-acetylglucosamine-3-O-lactic ether and UDP-N-acetylglucosamine-3-O-lactyl-peptides was observed under every condition which induced UDP-N-acetylglycosamine-3-O-lactyl-peptide accumulation. The relationship of the observed accumulation to the pathway of cell-wall mucopeptide synthesis is discussed.
在氮源剥夺、赖氨酸剥夺、奥霉素抑制和青霉素抑制等培养条件下，研究了金黄色葡萄球菌细胞内udp - n -乙酰氨基糖衍生物随时间的积累。在无氨基酸培养基中培养的细胞中积累了udp - n -乙酰氨基葡萄糖和udp - n -乙酰氨基葡萄糖-3- o -乳酸醚。在孵育的初始阶段，udp - n -乙酰氨基葡萄糖的出现迅速上升，随后该核苷酸减少;在诱导udp - n -乙酰氨基葡萄糖-3- o -乳酸醚和udp - n -乙酰氨基葡萄糖-3- o -乳酸肽积累的各种条件下，均观察到udp - n -乙酰氨基葡萄糖-3- o -乳酸肽的同时积累。讨论了观察到的积累与细胞壁粘肽合成途径的关系。
{"title":"Time course of accumulation of UDP-N-acetylamino sugar derivatives in Staphylococcus aureus","authors":"Eiji Ito, Masahiro Saito","doi":"10.1016/0006-3002(63)91634-2","DOIUrl":"10.1016/0006-3002(63)91634-2","url":null,"abstract":"<div><p>Under incubation conditions including deprivation of nitrogen sources, deprivation of lysine, inhibition by oxamycin and inhibition by penicillin, the accumulation of UDP-<em>N</em>-acetylamino sugar derivatives in <em>Staphylococcus aureus</em> cells, with time, has been studied. UDP-<em>N</em>-acetylglucosamine and UDP-<em>N</em>-acetylglucosamine-3-<em>O</em>-lactic ether accumulated in cells which were incubated in amino acid-free medium. During the initial phase of incubation there was a rapid rise in the appearance of UDP-<em>N</em>-acetylglucosamine followed later by a decrease of this nucleotide; concomitant accumulation of UDP-<em>N</em>-acetylglucosamine-3-<em>O</em>-lactic ether and UDP-<em>N</em>-acetylglucosamine-3-O-lactyl-peptides was observed under every condition which induced UDP-<em>N</em>-acetylglycosamine-3-<em>O</em>-lactyl-peptide accumulation. The relationship of the observed accumulation to the pathway of cell-wall mucopeptide synthesis is discussed.</p></div>","PeriodicalId":94301,"journal":{"name":"Biochimica et biophysica acta","volume":"78 2","pages":"Pages 237-247"},"PeriodicalIF":0.0,"publicationDate":"1963-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-3002(63)91634-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23683012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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