Abstract The aim of the study was to determine the effect of the perinatal period on redox status indicators in the blood of ewes before and after lambing and during lactation. The study was performed on 12 ewes of the synthetic SCP line. Blood for testing of redox parameters was collected seven times: before pregnancy, 1.5 months and 24 h before lambing, 2 and 24 h after lambing, and in the fourth and eighth weeks of lactation. The following blood indices were determined by spectrophotometry: lipid peroxides, malondialdehyde, superoxide dismutase, catalase, plasma total antioxidant capacity, uric acid, urea, bilirubin, and creatinine. The tests showed that during the perinatal period reactions are generated which lead to oxidative stress. Oxidative stress in pregnant ewes was found to increase during the period before lambing and may persist even up to weeks 4-8 of lactation.
{"title":"Redox status in the blood of ewes in the perinatal period and during lactation","authors":"K. Ognik, K. Patkowski, T. Gruszecki, K. Kostro","doi":"10.1515/BVIP-2015-0083","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0083","url":null,"abstract":"Abstract The aim of the study was to determine the effect of the perinatal period on redox status indicators in the blood of ewes before and after lambing and during lactation. The study was performed on 12 ewes of the synthetic SCP line. Blood for testing of redox parameters was collected seven times: before pregnancy, 1.5 months and 24 h before lambing, 2 and 24 h after lambing, and in the fourth and eighth weeks of lactation. The following blood indices were determined by spectrophotometry: lipid peroxides, malondialdehyde, superoxide dismutase, catalase, plasma total antioxidant capacity, uric acid, urea, bilirubin, and creatinine. The tests showed that during the perinatal period reactions are generated which lead to oxidative stress. Oxidative stress in pregnant ewes was found to increase during the period before lambing and may persist even up to weeks 4-8 of lactation.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"557 - 562"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67316720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Sienkiewicz, A. Szczurkowski, A. Dudek, J. Kaleczyc
Abstract The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whole-mount preparation. The tissues were cut into 12 μm-thick cryostat sections, and processed for double-immunofluorescence method. In all organs studied, the most abundant nerve fibres were dopamine β hydroxylase positive (DβH+). Some of them contained neuropeptide Y (NPY). Sporadically NPY-positive-only nerve fibres were found. Single DβH+ nerve terminals contained also galanine. Small numbers of the nerve fibres supplying studied organs were stained for substance P (SP) and calitonin gene related peptide (CGRP). Almost all SP+ fibres were also CGRP+, whereas single CGRP+ nerves were SPimmunonegative. Some nerve terminals were immunoreactive to vesicular acetylcholine transporter and vasoactive intestinal peptide. The organs studied were innervated unevenly. The highest density of the nerves was found in the areas of the tunica albuginea adjacent to the mesorchial border of the testis and their number gradually decreased towards the free border of the gonad. None of the vascular tissue of the testicular parenchyma was free of the nerve fibres, except sporadically encountered DβH+ nerves which supply seminiferous tubules. Within the head of the epididymis a moderate number of nerve terminals were found, but in the body and tail of the organ the number of nerves gradually increased. The vas deferens was supplied with very numerous nerve fibres. There were no differences in the density of the innervation between the funicular and abdominal part of the vas deferens.
摘要以6只雄性龙猫为研究对象。用乙醚麻醉，腹腔注射尼布塔尔加深麻醉。以0.4 L 4%的多聚甲醛缓冲液经心灌注龙猫，收集其睾丸、附睾和输精管。取每只栗鼠一个睾丸的白膜染色为全载制备。将组织切成12 μm厚的低温切片，采用双免疫荧光法处理。在所有器官中，多巴胺β羟化酶阳性(DβH+)的神经纤维最为丰富。部分含有神经肽Y (NPY)。偶有npy阳性的神经纤维。单DβH+神经末梢也含有丙氨酸。少量供血器官的神经纤维进行P物质(SP)和降钙素基因相关肽(CGRP)染色。几乎所有的SP+纤维也是CGRP+，而单个CGRP+神经是sp免疫阴性的。部分神经末梢对囊泡型乙酰胆碱转运体和血管活性肠肽有免疫反应。所研究的器官神经支配不均匀。在靠近睾丸系膜边界的白膜区域神经密度最高，在性腺自由边界处神经密度逐渐减少。睾丸实质的维管组织除偶见提供精小管的DβH+神经外，均无神经纤维存在。附睾头部有中等数量的神经末梢，但在附睾体和附睾尾部神经数量逐渐增加。输精管有大量的神经纤维。输精管索段与腹段的神经支配密度无明显差异。
{"title":"Innervation of the chinchilla testis, epididymis, and vas deferens","authors":"W. Sienkiewicz, A. Szczurkowski, A. Dudek, J. Kaleczyc","doi":"10.1515/BVIP-2015-0082","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0082","url":null,"abstract":"Abstract The study was performed on six male chinchillas. The animals were anaesthetised with ether and the anaesthesia was deepened with nembuthal injected intraperitoneally. The chinchillas were then transcardially perfused with 0.4 L of 4% buffered paraformaldehyde, and testes, epididymides, and vasa deferentia were collected. The tunica albuginea from one testis from each chinchilla was stained as whole-mount preparation. The tissues were cut into 12 μm-thick cryostat sections, and processed for double-immunofluorescence method. In all organs studied, the most abundant nerve fibres were dopamine β hydroxylase positive (DβH+). Some of them contained neuropeptide Y (NPY). Sporadically NPY-positive-only nerve fibres were found. Single DβH+ nerve terminals contained also galanine. Small numbers of the nerve fibres supplying studied organs were stained for substance P (SP) and calitonin gene related peptide (CGRP). Almost all SP+ fibres were also CGRP+, whereas single CGRP+ nerves were SPimmunonegative. Some nerve terminals were immunoreactive to vesicular acetylcholine transporter and vasoactive intestinal peptide. The organs studied were innervated unevenly. The highest density of the nerves was found in the areas of the tunica albuginea adjacent to the mesorchial border of the testis and their number gradually decreased towards the free border of the gonad. None of the vascular tissue of the testicular parenchyma was free of the nerve fibres, except sporadically encountered DβH+ nerves which supply seminiferous tubules. Within the head of the epididymis a moderate number of nerve terminals were found, but in the body and tail of the organ the number of nerves gradually increased. The vas deferens was supplied with very numerous nerve fibres. There were no differences in the density of the innervation between the funicular and abdominal part of the vas deferens.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"547 - 556"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67316893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kowalczyk, K. Urbaniak, I. Markowska-Daniel, Z. Pejsak
Abstract The aim of the study was to monitor genetic diversity and antigenic changes in the genome of influenza A(H1N1)pdm09 viral isolates detected during the post-pandemic period in Poland. Clinical specimens obtained from three suspected cases of influenza were analysed by sequencing. Among the differences identified in amino acids sequences, nine substitutions were located within the antigenic HA1 sites and in five residues forming receptor-binding pocket. The HA(D222G) mutation was shown in the isolate Swine/Poland/134312/12 obtained from a mild case of the disease. It must be emphasized that, in general, clinically mild cases are caused by the viruses in which that specific mutation, i.e. haemagglutinin (D222G), does not occur.
{"title":"Substitution in position 222 of haemagglutinin of pandemic influenza A (H1N1) viruses isolated from pigs in Poland","authors":"A. Kowalczyk, K. Urbaniak, I. Markowska-Daniel, Z. Pejsak","doi":"10.1515/BVIP-2015-0066","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0066","url":null,"abstract":"Abstract The aim of the study was to monitor genetic diversity and antigenic changes in the genome of influenza A(H1N1)pdm09 viral isolates detected during the post-pandemic period in Poland. Clinical specimens obtained from three suspected cases of influenza were analysed by sequencing. Among the differences identified in amino acids sequences, nine substitutions were located within the antigenic HA1 sites and in five residues forming receptor-binding pocket. The HA(D222G) mutation was shown in the isolate Swine/Poland/134312/12 obtained from a mild case of the disease. It must be emphasized that, in general, clinically mild cases are caused by the viruses in which that specific mutation, i.e. haemagglutinin (D222G), does not occur.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"7 1","pages":"451 - 456"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kędrak-Jabłońska, S. Budniak, A. Szczawińska, M. Reksa, M. Krupa, K. Szulowski
Abstract The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.
摘要本研究的目的是应用SYBR Green I插层染料荧光和TaqMan探针实时荧光定量PCR检测李斯特菌23S rDNA基因和单核增生李斯特菌hlyA基因，并进行比较。实验采用单增乳杆菌5株和各菌种的单株:L. ivanovii、L. innocua、L. grayi、L. welshimeri和L. seeligeri。此外，5株其他种类的细菌被用来评估测试的特异性。选用定量SYBR Green PCR试剂盒和定量Probe PCR试剂盒。在研究的第一阶段，采用几种方法进行SYBR Green I实时pcr，第一种方法允许检测23S rDNA基因，其余方法基于扩增hlyA基因。在接下来的部分中，进行了三种不同方法的基于TaqMan探针的实时pcr，可以确认李斯特菌属和单核增生李斯特菌属的菌株。实时荧光定量PCR方法通过观察扩增曲线可以检测到这两种基因，具有显著的敏感性和高特异性。所有反应的回归系数均为0.99。获得了23S rDNA和hlyA基因的特异性扩增产物，证实所分离菌株分别为李斯特菌属和单核增生李斯特菌属。其他微生物种类的分离物不能产生实时PCR产物。
{"title":"Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes","authors":"A. Kędrak-Jabłońska, S. Budniak, A. Szczawińska, M. Reksa, M. Krupa, K. Szulowski","doi":"10.1515/BVIP-2015-0073","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0073","url":null,"abstract":"Abstract The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"489 - 494"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of the study was to assess the seroprevalence of Coxiella burnetii in cattle herds in different regions of Poland. A total of 1150 serum samples collected from 443 cattle herds from 14 provinces were tested using complement fixation test. The seroprevalence was different in individual regions of Poland. The average percentage of seropositive herds was 40.41% and these herds were identified in each province tested.
{"title":"Seroprevalence of Coxiella burnetii in Polish cattle herds","authors":"A. Jodełko, K. Niemczuk, M. Szymańska-Czerwińska","doi":"10.1515/BVIP-2015-0071","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0071","url":null,"abstract":"Abstract The aim of the study was to assess the seroprevalence of Coxiella burnetii in cattle herds in different regions of Poland. A total of 1150 serum samples collected from 443 cattle herds from 14 provinces were tested using complement fixation test. The seroprevalence was different in individual regions of Poland. The average percentage of seropositive herds was 40.41% and these herds were identified in each province tested.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"479 - 482"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Jedziniak, M. Olejnik, J. Rola, T. Szprengier-Juszkiewicz
Abstract A multiresidue method (LC-MS/MS) for determination of wide range of anthelmintics was developed. The method covered benzimidazoles: albendazole (and metabolites), cambendazole, fenbendazol (and metabolites), flubendazole (and metabolites), mebendazole (and metabolites), oxibendazole, thiabendazole (and metabolites), triclabendazole (and metabolites); macrocyclic lactones: abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin; salicylanilides: closantel, ioxynil, nitroxynil, oxyclosamide, niclosamide, rafoxanid and others: clorsulon, derquantel, imidocarb, monepantel (and metabolites), morantel, praziquantel, and pyrantel. The method was used to examine the potential presence of anthelmintics in goat and sheep milk and dairy products from the Polish market. A total of 120 samples of milk, yoghurt, cottage cheese, cream cheese, and curd were analysed. None of the samples were found positive above CCα (1-10 μg/kg) except for one cottage cheese in which traces of albendazole sulfone were detected (5.2 ug/kg) and confirmed. The results of the study showed negligible anthelmintic residues in the goat and sheep milk and dairy products and confirm their good quality.
{"title":"Anthelmintic residues in goat and sheep dairy products","authors":"P. Jedziniak, M. Olejnik, J. Rola, T. Szprengier-Juszkiewicz","doi":"10.1515/BVIP-2015-0077","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0077","url":null,"abstract":"Abstract A multiresidue method (LC-MS/MS) for determination of wide range of anthelmintics was developed. The method covered benzimidazoles: albendazole (and metabolites), cambendazole, fenbendazol (and metabolites), flubendazole (and metabolites), mebendazole (and metabolites), oxibendazole, thiabendazole (and metabolites), triclabendazole (and metabolites); macrocyclic lactones: abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin; salicylanilides: closantel, ioxynil, nitroxynil, oxyclosamide, niclosamide, rafoxanid and others: clorsulon, derquantel, imidocarb, monepantel (and metabolites), morantel, praziquantel, and pyrantel. The method was used to examine the potential presence of anthelmintics in goat and sheep milk and dairy products from the Polish market. A total of 120 samples of milk, yoghurt, cottage cheese, cream cheese, and curd were analysed. None of the samples were found positive above CCα (1-10 μg/kg) except for one cottage cheese in which traces of albendazole sulfone were detected (5.2 ug/kg) and confirmed. The results of the study showed negligible anthelmintic residues in the goat and sheep milk and dairy products and confirm their good quality.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"515 - 518"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli O157 and E. coli O157:H7; therefore, herby cheese poses a serious risk to public health.
{"title":"Presence of Escherichia coli O157 and O157:H7 in raw milk and Van herby cheese","authors":"Y. C. Sancak, Hakan Sancak, O. İşleyi̇ci̇","doi":"10.1515/BVIP-2015-0076","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0076","url":null,"abstract":"Abstract The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli O157 and E. coli O157:H7; therefore, herby cheese poses a serious risk to public health.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"511 - 514"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67315642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Radko, M. Minta, A. Jasik, S. Stypuła-Trębas, J. Żmudzki
Abstract The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 μg/kg for 17α-ethinyloestradiol (s.c.); 1 μg/kg/day (s.c.) and 40 μg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 μg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.
{"title":"Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay","authors":"L. Radko, M. Minta, A. Jasik, S. Stypuła-Trębas, J. Żmudzki","doi":"10.1515/BVIP-2015-0080","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0080","url":null,"abstract":"Abstract The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 μg/kg for 17α-ethinyloestradiol (s.c.); 1 μg/kg/day (s.c.) and 40 μg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 μg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"533 - 540"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67316128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Wernicki, R. Urban-Chmiel, J. Rola, W. Socha, D. Stegierska, M. Dec, A. Puchalski
Abstract The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The presence of BHV-1 was confirmed by PCR in 42 (36.5%) animals. In the ELISA BHV1 antibody test, 39 (33.9%) seropositive animals were identified. The presence of BHV-1 positive samples was observed in all the examined breeds of young cattle. There were no significant differences (P ≤ 0.05) in BHV-1 positive samples. The results indicate that the incidence of BHV-1 infections in feedlot cattle herds studied was 32.2%-36.5%, which suggests that preventive measures should be implemented in order to limit transmission of the virus.
{"title":"Prevalence of bovine herpes virus type 1 in small herds of young beef cattle in south-eastern Poland – a preliminary study","authors":"A. Wernicki, R. Urban-Chmiel, J. Rola, W. Socha, D. Stegierska, M. Dec, A. Puchalski","doi":"10.1515/BVIP-2015-0069","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0069","url":null,"abstract":"Abstract The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The presence of BHV-1 was confirmed by PCR in 42 (36.5%) animals. In the ELISA BHV1 antibody test, 39 (33.9%) seropositive animals were identified. The presence of BHV-1 positive samples was observed in all the examined breeds of young cattle. There were no significant differences (P ≤ 0.05) in BHV-1 positive samples. The results indicate that the incidence of BHV-1 infections in feedlot cattle herds studied was 32.2%-36.5%, which suggests that preventive measures should be implemented in order to limit transmission of the virus.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"467 - 472"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/BVIP-2015-0069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.
{"title":"Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.)","authors":"E. Borzym, J. Maj-Paluch","doi":"10.1515/BVIP-2015-0070","DOIUrl":"https://doi.org/10.1515/BVIP-2015-0070","url":null,"abstract":"Abstract The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.","PeriodicalId":9462,"journal":{"name":"Bulletin of The Veterinary Institute in Pulawy","volume":"59 1","pages":"473 - 478"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67314991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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