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Effect of Body Posture on Lung Ventilation and Oxygenation During Carbon Dioxide Pneumoperitoneum in Rabbit 兔二氧化碳气腹时体位对肺通气和氧合的影响
Iulia Melega, Cosmina Dejescu, M. Dragomir, C. Danciu, F. Matei, L. Oana, C. Peștean
This study was conducted to investigate the influence of body position on respiratory compliance and oxygenation during iatrogenic pneumoperitoneum in the rabbit. The peak inspiratory pressure, dynamic compliance, static compliance and arterial gas parameters were calculated and measured 10 min before and 30 min after the creation of pneumoperitoneum with the patient in the horizontal position, 30 min after placing the patient in the Trendelenburg position and 30 min after placing the patient in the reversed Trendelenburg position. Following the creation of pneumoperitoneum and Trendelenburg positioning, there was a significant increase in peak inspiratory pressure while dynamic and static respiratory compliance decreased. Similarly, arterial oxygenation increased during Trendelenburg position while arterial carbon pressure remained within limits during all positions. Overall, the reverse Trendelenburg position did not improve ventilation, neither the oxygenation. However, this position showed to be more appropriate because may reduce the risk of lung injury associated with high-pressure ventilation during pneumoperitoneum.
本实验旨在探讨兔医源性气腹时体位对呼吸顺应性和氧合的影响。计算并测量患者水平体位气腹形成前10 min、后30 min、Trendelenburg体位放置后30 min、反Trendelenburg体位放置后30 min的峰值吸气压力、动态顺应性、静态顺应性和动脉气体参数。在气腹形成和Trendelenburg定位后,吸气峰值压力显著增加,而动态和静态呼吸顺应性下降。同样,在Trendelenburg体位期间,动脉氧合增加,而在所有体位期间动脉碳压保持在限制范围内。总的来说,反向Trendelenburg体位并没有改善通气,也没有改善氧合。然而,这种体位更合适,因为可以降低气腹期间高压通气引起肺损伤的风险。
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引用次数: 4
Removal of an Extensive Nasal Polyp in a Standing Horse, Using a Direct Approach to the Nasal Cavity Through a Bone Flap 通过骨瓣直接入路鼻腔切除站立马大面积鼻息肉
C. Crecan, I. Morar, M. Rus, M. Taulescu, L. Oana, C. Peștean
Nasal polyps are not very common in horses, comprising of just about 2,5 % of equine sinonasal disease (Tremaine and Dixon, 2001). However, nasal polyps can develop into extensive masses, obstructing partially or totally air flow thus giving dramatic clinical presentations. The aim of this report was to present a surgical technique used to successfully remove a massive nasal polyp in a standing mare. A 9 year old mixed-breed mare was referred to the equine teaching hospital of the Faculty of Veterinary Medicine in Cluj –Napoca for the evaluation of an extensive, unilateral nasal mass. Upon inspection, a cauliflower-appearing mass was protruding from the right nostril. A decision was made to try to surgically remove the mass using a direct approach through a nasal bone flap. Classic approach through the opening of the nostril was impossible due to the extent of the mass. The surgery was performed with the mare standing under sedation and regional anesthesia. The mass was approximately 30 cm by 7 cm and was described histologically as a nasal polyp. The mare made a full recovery, and there was no recurrence in the next three years after surgery.
鼻息肉在马中并不常见,仅占马鼻窦病的2.5% (Tremaine and Dixon, 2001)。然而,鼻息肉可发展成广泛的肿块,部分或全部阻塞气流,从而表现出戏剧性的临床表现。本报告的目的是介绍一种手术技术,用于成功地去除一个巨大的鼻息肉在一个站立的母马。一匹9岁的混血母马被转介到克鲁日纳波卡兽医学院的马教学医院,对广泛的单侧鼻肿块进行评估。经检查,右鼻孔有一菜花状肿块突出。我们决定尝试通过鼻骨瓣直接手术切除肿块。由于肿块的范围,通过鼻孔的传统入路是不可能的。手术是在镇静和局部麻醉下,母马站立进行的。肿块大小约为30cm × 7cm,在组织学上被描述为鼻息肉。这匹母马完全康复,在手术后的三年里没有复发。
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引用次数: 0
Feed Autonomy of a Few Dairy Cattle Farms in the Mitidja Plain (Algeria) 阿尔及利亚米蒂迪亚平原几个奶牛养殖场的饲料自主权
M. K. Ghozlane, S. Boukhechem, M. Bouamra
Feed autonomy of 15 dairy cattle farms was assessed during the 2019-2020 agricultural campaign. This autonomy is declined according to the food nature (fodder and concentrate) and their composition (dry matter, energy and protein). Autonomy is on average of 74.79% for dry matter, 75.10% for energy and 76.36% for protein. Fodder autonomy is on average of 93.58%, whereas for concentrate, the farms are 100% dependent on the external provision of feed. Overall, the farms produce fodder but in insufficient quantity, they therefore resort to the purchase of hay bales. This is explained by the weakness of the fodder sole and the high consumption of concentrate.
在2019-2020年农业活动期间,对15个奶牛养殖场的饲料自主性进行了评估。这种自主性根据食物性质(饲料和精料)及其组成(干物质、能量和蛋白质)而递减。干物质的平均自主权为74.79%,能量为75.10%,蛋白质为76.36%。饲料自主性平均为93.58%,而精料则100%依赖外部饲料供应。总的来说,农场生产饲料但数量不足,因此他们求助于购买干草捆。这是由于饲料底的缺点和精料的高消耗造成的。
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引用次数: 0
Histological and Morphometrical Study of the Filiform Papillae in the Domestic Rabbit (Oryctolagus cuniculus) 家兔(Oryctolagus cuniculus)丝状乳头的组织学和形态计量学研究
Mircea Cipou, C. Martonoș, A. Gal, V. Rus, I. Vlasiuc, V. Miclăuș, A. Damian
The domestic rabbit is often preferred as an experimental model in diverse research and especially those regarding pathologies of the oral cavity, both in human and veterinary medicine. From five domestic mixed breed rabbits, fragments were harvested from the three regions of the tongue: apex, body and root, for histological investigations. The samples were processed by embedding in paraffin, sectioned at a thickness of 5 µm and stained with Goldner’s trichrome method. The histological examination highlighted the presence of the filiform papillae on the entire dorsal surface of the tongue, with morphological particular carachteristics for each portion. The dimension of the filiform papillae of the rabbit’s tongue differs both regarding the length as well as the thickness and density. The shortest filiform papillae were found on the tip of the tongue, then the length increases progressively, reaching the maximum level on the middle portion and decreases in the last portion. The density also differs, in in the three compartments examined, being the highest in the anterior part and the lowest in the posterior part. The thickness of the filiform papillae is inversely proportional to their density. The thickness of the papillae is smaller where the density is higher and highest where the density is lowest. The upper surface of the filiform papillae is variably keratinized depending on the lingual segment. The papillae with the highest level of keratinization are arranged in the body part of the tongue, and those at the tip and root are slightly lower.
在各种研究中,特别是在人类和兽医学中有关口腔病理的研究中,家兔通常是首选的实验模型。从5只家兔的舌尖、舌体和舌根三个部位采集标本进行组织学研究。样品包埋石蜡处理,切片厚度为5µm, Goldner’s三色法染色。组织学检查显示,舌头整个背表面有丝状乳头状突起,每一部分都有独特的形态学特征。兔舌上丝状乳头的大小在长度、厚度和密度上都不同。丝状乳头在舌尖处最短,然后逐渐增加,在舌尖中部达到最大值,在舌尖末端逐渐减小。密度也不同,在检查的三个隔室中,前部最高,后部最低。丝状乳头的厚度与其密度成反比。密度高的地方乳头的厚度小,密度低的地方乳头的厚度大。根据舌节不同,丝状乳头的上表面有不同的角化。角化程度最高的乳突分布在舌体部分,舌尖和舌根的乳突略低。
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引用次数: 2
First Pathological, Molecular and Serological Investigation of Ovine Johne's Disease (Paratuberculosis) in Northeastern Algeria 阿尔及利亚东北部羊约翰氏病(副结核)的首次病理、分子和血清学调查
F. Rezig, R. Bouzid, K. Atia, L. Aoun
Paratuberculosis (PTCB) or Johne's disease (JD) caused by a slow growing acid fast bacillus Mycobacterium avium subspecies paratuberculosis (MAP), is an infectious disease of many species including humans. The disease is responsible for important economic losses to livestock industry worldwide. Although the disease is widespread, it had not been studied in Algerian sheep. In this study we inspected the presence of the infection in sheep (aged 2 years old or older) using histopathology and IS900-PCR techniques in randomly 378 tissues (ileum, ileoceacale valve and lymph nodes) and fecal samples. Gross lesions were detected in 16 (4%) of samples. Histopathological examination revealed the presence of pathognomonic lesions of JD in 61 (14%) animals. Ziehl-Neelsen (ZN) staining of tissue samples was positive in 28 (7%) cases. MAP-DNA was detected in 34 (9%) fecal samples. 14 (4%) animals were tested positive for antibodies against MAP. This study showed that not only ovine paratuberculosis present in Algeria but infected animals are excreting the bacteria in feces. This could be a significant threat for other ruminants and humans. Other studies should be carried out in order to better understand the prevalence and the molecular epidemiology of MAP in the country.
副结核(pttcb)或约翰氏病(JD)是一种由生长缓慢的抗酸鸟分枝杆菌亚种副结核(MAP)引起的传染病,是包括人类在内的许多物种的传染病。该疾病对全世界畜牧业造成重大经济损失。虽然这种疾病很普遍,但还没有在阿尔及利亚羊身上进行过研究。在这项研究中,我们使用组织病理学和IS900-PCR技术对随机378个组织(回肠、回肠瓣和淋巴结)和粪便样本进行了感染检测(2岁或以上)。在16例(4%)样本中检测到肉眼病变。组织病理学检查显示61只(14%)动物存在JD的病理病变。组织标本Ziehl-Neelsen (ZN)染色阳性28例(7%)。在34份(9%)粪便样本中检测到MAP-DNA。14只(4%)动物MAP抗体检测呈阳性。这项研究表明,阿尔及利亚不仅存在绵羊副结核病,而且受感染的动物也在粪便中排泄细菌。这可能对其他反刍动物和人类构成重大威胁。还应开展其他研究,以便更好地了解该国MAP的患病率和分子流行病学。
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引用次数: 0
Anatomical, Histological and Histochemical Features of the Guinea Pig (Cavia porcellus) Caecum 豚鼠(Cavia porcellus)盲肠的解剖、组织学和组织化学特征
A. Chende, C. Martonoș, A. Gal, V. Rus, V. Miclăuș, Dalma Pivariu, I. Vlasiuc, S. Andrei, A. Damian
In this study, the caecum of five guinea pigs was anatomically, histologically, and histochemically analyzed. From an anatomical point of view, it has been proved that the caecum in guinea pigs occupies the caudal segment of the abdominal cavity and consists of three parts: the ampullary portion, the body of the caecum, and the apex of the caecum, without a caecal appendix. In our histological analysis, we observed that the caecum has a simple structure, and the cecal mucosal glands are rare and contain, in addition to enterocytes, a small number of goblet cells, which are better represented in the deep part of the glands. Histochemically it has been observed that goblet cells are PAS and Alcian blue positive, which shows that they secrete both neutral and acidic mucins. The intensity of these two histochemical reactions is similar to that of goblet cells from other intestinal segments, proving that they are typical goblet cells. The large volume of the caecum suggests that this is an important section for the digestion process, although the relatively simple structure of the caecal mucosa suggests that the digestion here is not preponderant, but only complements the intestinal one.
本研究对5只豚鼠的盲肠进行了解剖、组织学和组织化学分析。从解剖学的角度来看,已经证明豚鼠的盲肠位于腹腔尾段,由壶腹部分、盲肠体和盲肠顶端三部分组成,没有盲肠阑尾。在我们的组织学分析中,我们观察到盲肠结构简单,盲肠粘膜腺很少,除了肠细胞外,还含有少量杯状细胞,杯状细胞在腺体的深部更有代表性。组织化学上观察到杯状细胞PAS和阿利新蓝阳性,表明它们分泌中性和酸性粘蛋白。这两种组织化学反应的强度与其他肠段的杯状细胞相似,证明它们是典型的杯状细胞。盲肠的大体积表明这是消化过程的重要部分,尽管盲肠黏膜相对简单的结构表明这里的消化不是优势,而只是补充肠道的消化。
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引用次数: 1
Acute Renal Failure and Laminitis Following Cesarean Section in a Friesian Mare. A Case Report 剖宫产后急性肾衰竭和叶板炎。病例报告
Z. Daradics, M. Rus, Antonia Popa, C. Crecan, C. Peștean, M. Mircean, C. Catoi, I. Morar
The term dystocia refers to an abnormal birth and the most common cause is an abnormal alignment of the head or forelimbs in the birth canal. Usually in adult horses, acute renal failure occurs as a complication of another disease process that causes hypovolemia . Diarrhea and severe laminitis may develop in more serious cases. The aim of this case report was to present a cesarean section in the mare that evolved with renal failure and subsequent with laminitis. A nine year old Friesian mare was referred to the Veterinary teaching hospital FMV Cluj-Napoca, after a dystocia that could not be resolved conservatively.During surgery, profuse hemorrhage was noticed during the incision and suture of the uterine wall. Hypovolemia was corrected and recovery was uneventful. Two days after surgery, the mare developed acute hyposthenuric renal failure. After 3 days of intensive therapy, kidney function started to improve but the mare developed laminitis. The mare improved over a few weeks and was discharged after orthopedic shoeing. According to the author’s knowledge, this is the first case report of a cesarean section in the mare that evolved into this cascade of sequelae.
难产是指异常分娩,最常见的原因是头部或前肢在产道中的异常排列。通常在成年马中,急性肾衰竭是另一种引起低血容量的疾病过程的并发症。更严重的病例可能会出现腹泻和严重的板层炎。本病例报告的目的是提出一个剖宫产的母马演变与肾功能衰竭,随后与板炎。一只九岁的弗里西亚母马因难产而无法保守治疗,被转介到克卢日纳波卡兽医教学医院。术中发现子宫壁切开缝合时大量出血。低血容量得到纠正,恢复顺利。术后2天,母马出现急性低尿肾衰。强化治疗3天后,肾功能开始改善,但母马出现板膜炎。几周后,母马病情有所改善,并在矫形鞋后出院。据作者所知,这是第一例剖宫产在母马演变成这种级联后遗症的病例报告。
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引用次数: 0
Effect of Sprouted Trigonella foenum graecum L. Incorporation into the Diet on Milk Production of Rabbit Does and Growth of Young Rabbits in the Northeast of Algeria 阿尔及利亚东北部地区日粮中添加发芽葫芦巴对家兔产奶量和幼兔生长的影响
S. Boukhechem, Hithem Bougherara, N. Mimoune, Roumeissa Redouane, N. nia, R. Kaidi
The aim of this study was to assess the effect of sprouted fenugreek incorporation into the diet of rabbits on their dairy and growth performances. Data from 24 lactations performed by 8 Californian rabbit does were studied. They were divided into 2 homogeneous groups, a control (batch C) having received an ordinary ration and an experimental (batch E) having received the same ration supplemented with sprouted fenugreek. Growth rates of suckling pups were also recorded. Then, Growth performance of 152 young rabbits’ issue from these females was monitored. They were divided into 4 homogeneous groups, one control having received an ordinary diet (batch C) and 3 experimental having received the same diet with substitution of 12%, 15% and 25% (batch E1, E3 and E3) of the concentrate by sprouted fenugreek. The results showed an improvement in the dairy performance of the rabbit does of batch E, with an average peak lactation of 287 g/d vs 236 g/d in batch C (p <0.01). The daily weight gain of suckling pups was better in batch E (13 g/d vs 11.7 g/d; p <0.01). Likewise, the weaned young rabbits of the experimental batches recorded higher growth rates than the control batch (p <0.01). Sprouted fenugreek incorporation into the rabbit’s ration allowed to improve their performances.
本研究的目的是评估胡芦巴芽加入家兔日粮对其乳制品和生长性能的影响。研究了8只加利福尼亚家兔24次泌乳的数据。将其分为2个均匀组,对照组(C批)给予普通日粮,试验组(E批)给予相同日粮中添加葫芦巴芽。还记录了哺乳幼仔的生长速度。然后,对这些母兔所产152只幼兔的生长性能进行监测。随机分为4个均匀组,1个对照组饲喂普通饲粮(C批),3个试验组饲喂相同饲粮,分别用葫芦巴芽代替12%、15%和25% (E1、E3和E3批)的精料。结果表明,E批兔的产乳性能显著提高,平均泌乳峰值为287 g/d,而C批为236 g/d (p <0.01)。E批乳仔日增重较好(13 g/d vs 11.7 g/d);p < 0.01)。试验组断奶幼兔的生长速率高于对照组(p <0.01)。在家兔日粮中加入胡芦巴芽可以提高家兔的生产性能。
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引用次数: 1
Antimicrobial Susceptibility Profile of Bacteria in Mastitis Cow Milk Samples 乳腺炎牛奶样品中细菌的抗菌敏感性分析
G. Nadăș, C. Novac, C. Bouari, I. Matei, N. Fiț
Bovine mastitis is a cause of major financial losses in the dairy industry worldwide. Bovine mastitis is defined as the inflammatory response of the udder tissue due to either physical trauma or infections caused by different microorganisms, especially bacteria. More than 135 pathogenic agents have been identified from bovine mastitis. The aim of the study was to identify the bacterial genera involved in mastitis etiology in cattle and to test the antimicrobial susceptibility to antibiotics. The samples were collected before morning milking and evaluated by microscopic and cultural examination, and further identified using biochemical methods. The evaluation of antimicrobial susceptibility was performed using Kirby-Bauer disk diffusion test. Overall, Staphylococcus spp., Bacillus spp., Streptococcus spp., Micrococcus spp., Escherichia spp. and Trueperella spp. were identified. The most frequent bacterial associations were observed for Staphylococcus-Bacillus, Bacillus-Streptococcus and Staphylococcus-Micrococcus. Regarding the antimicrobial sensitivity profile, the majority of the bacterial strains we have isolated were classified as sensitive, with the exception of Escherichia coli, with one strain being resistant to all tested antibiotics. Among the antimicrobials, the Amoxicillin and clavulanic acid was the most efficient one and Trimethoprim/Sulfamethoxazole did not exhibit an inhibitory activity in 19 milk samples.
牛乳腺炎是造成全球乳制品行业重大经济损失的原因之一。牛乳腺炎被定义为由于物理创伤或由不同微生物,特别是细菌引起的感染而引起的乳房组织的炎症反应。目前已鉴定出135种以上的牛乳腺炎致病菌。本研究的目的是鉴定奶牛乳腺炎病原学中涉及的细菌属,并测试对抗生素的抗菌敏感性。在早晨挤奶前采集样品,通过显微镜和培养检查进行鉴定,并进一步使用生化方法进行鉴定。采用Kirby-Bauer盘片扩散试验评价药敏。总体上鉴定出葡萄球菌、芽孢杆菌、链球菌、微球菌、埃希氏菌和真佩尔氏菌。葡萄球菌-芽孢杆菌、芽孢杆菌-链球菌和葡萄球菌-微球菌最常见。在抗菌药物敏感性方面,除大肠杆菌外,我们分离的大多数细菌菌株都被归类为敏感菌株,其中一株菌株对所有测试的抗生素都具有耐药性。其中,阿莫西林和克拉维酸的抗菌效果最好,而甲氧苄啶/磺胺甲恶唑对19份牛奶样品没有抑制作用。
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引用次数: 1
Zootechnical Fattening Protocol for Mixed Breed Young Bulls with Zoo-sanitary Management System 采用动物园卫生管理系统的杂交公牛动物技术育肥方案
H. Saoudi, Amel Saoudi, Adila Fartas, L. Aoun
The objective of this study is to reduce the cost of raising beef cattle in Algeria by proposing a Zootechnical fattening protocol for breeders. The study was carried out on mixed-breed young bulls (Charolais x local breeds). Food rations were distributed daily; the first ration (F1) was made up of soybeans while the second ration (F2) was made of tomato pulp supplemented with sodium bicarbonate. Parameters calculated were the Average Daily Gain (ADG), quantity of faeces eliminated, and weight of each bull. The ADG results shown in F1 and F2 are close. The addition of Sodium Bicarbonate in the F2 ration helps to alleviate certain digestive disorders, particularly acidosis and diarrhoea; therefore, reducing the production costs per kg of meat obtained from bulls fed with tomato which was available annually. The proposed protocol improved fillet yield and lowered the cost per kg of meat. It can be applied internationally in any rudimentary livestock farm.
本研究的目的是通过为饲养者提出动物技术育肥方案来降低阿尔及利亚饲养肉牛的成本。本研究以夏来与地方品种杂交的公公牛为研究对象。每天分发口粮;第1日粮(F1)由大豆组成,第2日粮(F2)由番茄浆和碳酸氢钠组成。计算的参数包括平均日增重(ADG)、排便量和每头公牛的体重。F1和F2的ADG结果接近。在F2日粮中添加碳酸氢钠有助于缓解某些消化系统疾病,特别是酸中毒和腹泻;因此,降低了每年用番茄喂养的公牛每公斤肉的生产成本。该方案提高了鱼片产量,降低了每公斤肉的成本。它可以在国际上任何一个初级牲畜养殖场应用。
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引用次数: 0
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
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