Pub Date : 2022-11-14DOI: 10.15835/buasvmcn-vm:2022.0010
Anca Forgaciu, Ingrid-Alexandra ROTAR-MENYHART, Corina Beres, Anette Iudit GHEITI HEGHEDUS, Andrei Greapcă, R. Mihaiu, I. Pașca, M. Mihaiu
The frequency of salmonellosis in Europe remains high, being considered the second most common foodborne disease. Salmonella spp., was most commonly detected in poultry meat mainly in fresh turkey meat (3.5%) and fresh chickens (2.2%). The study aims to identify the level of contamination of poultry meat with Salmonella spp. and performing a risk assessment. The samples were collected from a large capacity slaughterhouse on two slaughter lines, from two groups of poultry. An antemortem and postmortem examination was conducted and the samples collected from the neck of poultry carcasses were analyzed using the VIDAS® bioMérieux automatic test using analytical technique. The results of the antemortem inspection indicated negative results. The postmortem examination on group I and group II indicated different types of lesions. The surveillance of contamination of poultry meat with germs of the genus Salmonella spp. highlighted 397 compliant samples in group I, out of a total of 403 samples and 6 non-compliant samples. Salmonella infantis was identified in these non-compliant samples following laboratory examination. The lesions associated with Salmonella infection are not specific and therefore there is no direct correlation between the results of laboratory examination and lesions.
{"title":"Risk Assessment Regarding the Contamination of Poultry Meat with Salmonella in a High-Capacity Slaughterhouse","authors":"Anca Forgaciu, Ingrid-Alexandra ROTAR-MENYHART, Corina Beres, Anette Iudit GHEITI HEGHEDUS, Andrei Greapcă, R. Mihaiu, I. Pașca, M. Mihaiu","doi":"10.15835/buasvmcn-vm:2022.0010","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2022.0010","url":null,"abstract":"The frequency of salmonellosis in Europe remains high, being considered the second most common foodborne disease. Salmonella spp., was most commonly detected in poultry meat mainly in fresh turkey meat (3.5%) and fresh chickens (2.2%). The study aims to identify the level of contamination of poultry meat with Salmonella spp. and performing a risk assessment. The samples were collected from a large capacity slaughterhouse on two slaughter lines, from two groups of poultry. An antemortem and postmortem examination was conducted and the samples collected from the neck of poultry carcasses were analyzed using the VIDAS® bioMérieux automatic test using analytical technique. The results of the antemortem inspection indicated negative results. The postmortem examination on group I and group II indicated different types of lesions. The surveillance of contamination of poultry meat with germs of the genus Salmonella spp. highlighted 397 compliant samples in group I, out of a total of 403 samples and 6 non-compliant samples. Salmonella infantis was identified in these non-compliant samples following laboratory examination. The lesions associated with Salmonella infection are not specific and therefore there is no direct correlation between the results of laboratory examination and lesions.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72432955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-14DOI: 10.15835/buasvmcn-vm:2022.0014
Cristiana Genes, Cristian Mărgărit, R. Bălăceanu, N. Dojană
The paper aimed to present the evolution of food intake, body weight, laying performance, mortality, ovary and oviduct weight and protein metabolism in 63-week-old natural moulted Hy-Line var. Brown hens housed in industrial system. Ingested crude protein, serum protein, albumin, globulins, uric acid, and total manure nitrogen were monitored for eight weeks of moult to characterize the protein metabolism of the hens. Spontaneous moult of the Hy-Line Brown hybrid is characterized by a decrease in food intake by up to 35.0%, a decrease in body weight by up to 31.6%, a significant increase in mortality (1.45% vs. 0.66% in non moulting hens), by stopping of the laying process, a 67.2% decrease of the ovary weight and 54.3% decrease of the oviduct weight versus non-moulted gens. The serum level of uric acid was increased during the period of massive moult revealing a high protein catabolism. Total nitrogen manure was decreased during the period of massive moult, a fact attributed to the decrease in food intake. Eight weeks after the onset of the spontaneous moult process, the reported changes of moulted hens were no longer significantly different from those of non-moulted hens.
{"title":"Body Characteristics, Performances and Protein Metabolism in Hy-Line Brown Hens Subject of Natural Moult","authors":"Cristiana Genes, Cristian Mărgărit, R. Bălăceanu, N. Dojană","doi":"10.15835/buasvmcn-vm:2022.0014","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2022.0014","url":null,"abstract":"The paper aimed to present the evolution of food intake, body weight, laying performance, mortality, ovary and oviduct weight and protein metabolism in 63-week-old natural moulted Hy-Line var. Brown hens housed in industrial system. Ingested crude protein, serum protein, albumin, globulins, uric acid, and total manure nitrogen were monitored for eight weeks of moult to characterize the protein metabolism of the hens. Spontaneous moult of the Hy-Line Brown hybrid is characterized by a decrease in food intake by up to 35.0%, a decrease in body weight by up to 31.6%, a significant increase in mortality (1.45% vs. 0.66% in non moulting hens), by stopping of the laying process, a 67.2% decrease of the ovary weight and 54.3% decrease of the oviduct weight versus non-moulted gens. The serum level of uric acid was increased during the period of massive moult revealing a high protein catabolism. Total nitrogen manure was decreased during the period of massive moult, a fact attributed to the decrease in food intake. Eight weeks after the onset of the spontaneous moult process, the reported changes of moulted hens were no longer significantly different from those of non-moulted hens.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73083774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-14DOI: 10.15835/buasvmcn-vm:2022.0021
H. Aryan, O. Mardenli, I. Groza
The current study aimed to study the feasibility of in vitro maturation of equine oocytes during the breeding and non-breeding seasons by using the ovarian scraping method. The collection of 752 ovaries resulted in 608 oocytes descending from 2824 ovarian follicles by the method of ovarian scraping at a rate of 21.53% and an average of 0.80 oocyte/ovary. During the breeding season, 432 oocytes were collected whereas 176 oocytes were collected in the non-breeding season. The results showed that the number of cultivable oocytes during the breeding season was 256 oocytes (59.25%), while the number of cultivable oocytes in the non-breeding one was 120 oocytes (68.18%) at the level of p <0.001. The application of oocyte collecting by scraping method allowed obtaining a number of 608 oocytes, of which a number of 376 oocytes (61.84%) were cultivable, the rest of 232 oocytes (38.16%) were classified as uncultivable. It is concluded from the current study that the application of the ovarian scraping method in horses contributes to obtaining a high and encouraging rate of oocytes that can be used in in vitro embryo production programs.
{"title":"Effectiveness of the Ovarian Scraping Method in In Vitro Maturation of Equine Oocytes During the Breeding and Non-Breeding Seasons","authors":"H. Aryan, O. Mardenli, I. Groza","doi":"10.15835/buasvmcn-vm:2022.0021","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2022.0021","url":null,"abstract":"The current study aimed to study the feasibility of in vitro maturation of equine oocytes during the breeding and non-breeding seasons by using the ovarian scraping method. The collection of 752 ovaries resulted in 608 oocytes descending from 2824 ovarian follicles by the method of ovarian scraping at a rate of 21.53% and an average of 0.80 oocyte/ovary. During the breeding season, 432 oocytes were collected whereas 176 oocytes were collected in the non-breeding season. The results showed that the number of cultivable oocytes during the breeding season was 256 oocytes (59.25%), while the number of cultivable oocytes in the non-breeding one was 120 oocytes (68.18%) at the level of p <0.001. The application of oocyte collecting by scraping method allowed obtaining a number of 608 oocytes, of which a number of 376 oocytes (61.84%) were cultivable, the rest of 232 oocytes (38.16%) were classified as uncultivable. It is concluded from the current study that the application of the ovarian scraping method in horses contributes to obtaining a high and encouraging rate of oocytes that can be used in in vitro embryo production programs.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73325870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0019
Vlad Lupu, Iulia Gabriela Pușcoi, N. Tudor
Gastro-esophageal reflux is the return of gastric contents in the esophagus, phenomenon that can be identified in dogs under general anesthesia, but also in dogs without anesthesia, due to disorders of the distal esophageal sphincter. The aim of the present study was the comparative assessment of the incidence of gastro-esophageal reflux in brachycephalic and non-brachycephalic dogs. The medical records were evaluated for 98 dogs, presented with different pathologies of the spine. The animals were subjected to a standardized anesthetic protocol and CT scan. Following the analysis of the acquired CT images, it was found that gastro-esophageal reflux was present in 31 brachycephalic dogs (31/46; 67.39%) and in 9 non-brachycephalic dogs (9/52; 17.31%), respectively. There was an increased predisposition of the brachycephalic breeds regarding the occurrence of gastro-esophageal reflux, using identical anesthesia and positioning protocols.
{"title":"Retrospective Study on the Prevalence of Gastro-esophageal Reflux in Brachycephalic and Non-Brachycephalic Dogs Anesthetized for CT Examination","authors":"Vlad Lupu, Iulia Gabriela Pușcoi, N. Tudor","doi":"10.15835/buasvmcn-vm:2021.0019","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0019","url":null,"abstract":"Gastro-esophageal reflux is the return of gastric contents in the esophagus, phenomenon that can be identified in dogs under general anesthesia, but also in dogs without anesthesia, due to disorders of the distal esophageal sphincter. The aim of the present study was the comparative assessment of the incidence of gastro-esophageal reflux in brachycephalic and non-brachycephalic dogs. The medical records were evaluated for 98 dogs, presented with different pathologies of the spine. The animals were subjected to a standardized anesthetic protocol and CT scan. Following the analysis of the acquired CT images, it was found that gastro-esophageal reflux was present in 31 brachycephalic dogs (31/46; 67.39%) and in 9 non-brachycephalic dogs (9/52; 17.31%), respectively. There was an increased predisposition of the brachycephalic breeds regarding the occurrence of gastro-esophageal reflux, using identical anesthesia and positioning protocols. ","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83402128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0033
B. Tudor, O. Boldura, C. Mircu, C. Tulcan, S. Marc, Ovidiu Georgescu, I. Huțu
Microfluidic electrophoresis (ELFO) has become a widely applicable method that allows the analysis of proteins fractions. ELFO presents advantages that include time reduction low sample consumption, high method efficiency and the integration of results in the context. With sample proper preparation the method becomes accurate and robust and the reagents and consumables used are considerably reduced. This paper describes the adaptation and improvement of a raw milk protein analysis protocol, reported in the standard microfluidic electrophoresis analysis procedures of Agilent Technologies. The described protocol makes adaptations regarding the preparation of biological samples composed of raw milk in the pre-analytical stage with multiple benefits in reporting the final results. Besides the proteins, in the composition of milk, significant percent of fats could be found and the described procedure is practically another step required for the elimination of those interfering substances. This method involves the pre-analytical stage of preparation of raw milk samples, separation and quantification of major milk proteins and total protein content. The improvement proved to have an important beneficial impact on the analysis as the result was very accurate in terms of quantity and quality and also it has drastically reduced the errors in data interpretation of the equipment.
{"title":"Use of Microfluidic Electrophoresis in the Analysis of Milk Proteins- Method Improvement","authors":"B. Tudor, O. Boldura, C. Mircu, C. Tulcan, S. Marc, Ovidiu Georgescu, I. Huțu","doi":"10.15835/buasvmcn-vm:2021.0033","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0033","url":null,"abstract":"Microfluidic electrophoresis (ELFO) has become a widely applicable method that allows the analysis of proteins fractions. ELFO presents advantages that include time reduction low sample consumption, high method efficiency and the integration of results in the context. With sample proper preparation the method becomes accurate and robust and the reagents and consumables used are considerably reduced. This paper describes the adaptation and improvement of a raw milk protein analysis protocol, reported in the standard microfluidic electrophoresis analysis procedures of Agilent Technologies. The described protocol makes adaptations regarding the preparation of biological samples composed of raw milk in the pre-analytical stage with multiple benefits in reporting the final results. Besides the proteins, in the composition of milk, significant percent of fats could be found and the described procedure is practically another step required for the elimination of those interfering substances. This method involves the pre-analytical stage of preparation of raw milk samples, separation and quantification of major milk proteins and total protein content. The improvement proved to have an important beneficial impact on the analysis as the result was very accurate in terms of quantity and quality and also it has drastically reduced the errors in data interpretation of the equipment.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87645910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0025
Iuliu Torda, Irina-Ioana Spataru, G. Otavă, S. Marc, O. Boldura, I. Huțu, B. Lungu, B. Tudor, Ovidiu Georgescu, C. Mircu
Mitochondria is the most frequently studied cell organelle in relation to development competence of the oocytes both in vivo and in vitro. Developmental competence of oocyte is crucial for embryonic development, the molecular activity until the activation of the embryonic genome being based on the maternal reserves stored in the oocyte. Study was designed to qualitatively evaluate 133 COC class C1 and class C2 gilts and sow’s oocytes according to the distribution of mitochondria after IVM, based on Rhodamine 123 stain solution. Regarding mitochondrial distribution, the percentage of oocytes that had a homogeneous distribution around the germinal vesicle was higher in group C1 than C2 and also higher in sow oocytes than in gilts oocytes. For class 1, the difference was 10.58% in favor of sows, the same trend is maintained for C2, the difference being 4.37%. The results are confirmed by morphological examination, where C1 sow’s oocytes matured 26.09% more, compared to gilts oocytes, a difference maintained for C2 oocytes, being 27.4% more sow’s oocytes IVM compared to gilts. Based on mitochondrial distribution we observed that the stage of sexual development of females influences the IVM of oocytes. For pig’s IVF it’s recommended to use C1 sow oocytes.
{"title":"Quality Evaluation of Gilts and Sow’s Oocytes During In Vitro Maturation Based on Mitochondria Distribution","authors":"Iuliu Torda, Irina-Ioana Spataru, G. Otavă, S. Marc, O. Boldura, I. Huțu, B. Lungu, B. Tudor, Ovidiu Georgescu, C. Mircu","doi":"10.15835/buasvmcn-vm:2021.0025","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0025","url":null,"abstract":"Mitochondria is the most frequently studied cell organelle in relation to development competence of the oocytes both in vivo and in vitro. Developmental competence of oocyte is crucial for embryonic development, the molecular activity until the activation of the embryonic genome being based on the maternal reserves stored in the oocyte. Study was designed to qualitatively evaluate 133 COC class C1 and class C2 gilts and sow’s oocytes according to the distribution of mitochondria after IVM, based on Rhodamine 123 stain solution. Regarding mitochondrial distribution, the percentage of oocytes that had a homogeneous distribution around the germinal vesicle was higher in group C1 than C2 and also higher in sow oocytes than in gilts oocytes. For class 1, the difference was 10.58% in favor of sows, the same trend is maintained for C2, the difference being 4.37%. The results are confirmed by morphological examination, where C1 sow’s oocytes matured 26.09% more, compared to gilts oocytes, a difference maintained for C2 oocytes, being 27.4% more sow’s oocytes IVM compared to gilts. Based on mitochondrial distribution we observed that the stage of sexual development of females influences the IVM of oocytes. For pig’s IVF it’s recommended to use C1 sow oocytes.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88374834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0022
Maria Cătălina MATEI LAȚIU, V. Buza, F. Chirilǎ, Z. Boros, C. Lațiu, A. Szakacs, L. Ștefănuț
Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts, with the main site of action at the intestinal level. In order to have a maximal effect, probiotic bacteria have to prove a high tolerance for the conditions present in the upper gastro-intestinal tract. The aim of the present study was to assess, from a qualitative point of view, the tolerance of different probiotic bacterial strains and combinations to simulated gastric juice, bile, fructose, lactose and glucose. Also, the influence of food on the bacterial growth after artificial digestion was analyzed. A bacteriologic assessment was realized for three different commercial probiotic products (Bacillus subtilis + Bacillus licheniformis + Pediococcus acidilactici; Enterococcus faecium; Bacillus subtilis + B. licheniformis + B.coagulans + B.indicus + B. clausii), simulating different conditions (artificial digestion method in the absence/presence of food, presence of bile, fructose, glucose, lactose). The obtained results suggested that the tolerance to simulated gastric juice is higher if the food is present in the moment of digestion, regardless of the probiotic bacterial strain or combination. Also, the tolerance to bile was different from one combination to another. The influence of fructose, lactose and glucose are not remarkable from a qualitative point of view. Moreover, the growth of different probiotic bacterial strains is influenced by the simulated gastro-intestinal conditions, sporulated bacteria presenting a slightly higher tolerance compared with non-sporulated strains.
{"title":"In Vitro Qualitative Assessment of Tolerance to Simulated Gastric Juice, Bile, Fructose, Glucose and Lactose for Different Probiotic Bacteria","authors":"Maria Cătălina MATEI LAȚIU, V. Buza, F. Chirilǎ, Z. Boros, C. Lațiu, A. Szakacs, L. Ștefănuț","doi":"10.15835/buasvmcn-vm:2021.0022","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0022","url":null,"abstract":"Probiotics are live microorganisms that confer a health benefit to the host when administered in adequate amounts, with the main site of action at the intestinal level. In order to have a maximal effect, probiotic bacteria have to prove a high tolerance for the conditions present in the upper gastro-intestinal tract. The aim of the present study was to assess, from a qualitative point of view, the tolerance of different probiotic bacterial strains and combinations to simulated gastric juice, bile, fructose, lactose and glucose. Also, the influence of food on the bacterial growth after artificial digestion was analyzed. A bacteriologic assessment was realized for three different commercial probiotic products (Bacillus subtilis + Bacillus licheniformis + Pediococcus acidilactici; Enterococcus faecium; Bacillus subtilis + B. licheniformis + B.coagulans + B.indicus + B. clausii), simulating different conditions (artificial digestion method in the absence/presence of food, presence of bile, fructose, glucose, lactose). The obtained results suggested that the tolerance to simulated gastric juice is higher if the food is present in the moment of digestion, regardless of the probiotic bacterial strain or combination. Also, the tolerance to bile was different from one combination to another. The influence of fructose, lactose and glucose are not remarkable from a qualitative point of view. Moreover, the growth of different probiotic bacterial strains is influenced by the simulated gastro-intestinal conditions, sporulated bacteria presenting a slightly higher tolerance compared with non-sporulated strains.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77396618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0034
B. Tudor, O. Boldura, C. Mircu, C. Tulcan, S. Marc, B. Lungu, I. Torda, I. Huțu
Dairy products are an essential component of human nutrition and therefore are prone to adulteration. Although many methods have been developed based on the analysis of protein substances by which adulteration is detected, they have low efficiency in the case of processed products. In this context, methods based on DNA analysis have become the best choice, although the results are not universally valid and must be adapted to the composition and local specificities. Here is presented a study of the applicability of a fast and cost-effective DNA-based method to determine the species composition of dairy products by performing a qualitative triplex PCR detection technique in the analysis of local dairy products. The procedure is adapted to the domestic market and considers experimental models developed and well defined internationally. In the in-house validation of this method, three species of ruminants were considered: beef, sheep, and goat. The reference material was represented by cheeses prepared in our laboratory in strictly controlled percentage mixtures. Total genomic DNA isolated from the reference cheeses were used to develop a triplex PCR method, which involves the simultaneous detection of the three species studied using a mixture of three pairs of primers. The reference materials proved to be optimal for such studies and have been used successfully in the process of validating the method of detecting the composition of commercial cheeses.
{"title":"A Molecular Procedure for Detecting Dairy Products Adulteration","authors":"B. Tudor, O. Boldura, C. Mircu, C. Tulcan, S. Marc, B. Lungu, I. Torda, I. Huțu","doi":"10.15835/buasvmcn-vm:2021.0034","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0034","url":null,"abstract":"Dairy products are an essential component of human nutrition and therefore are prone to adulteration. Although many methods have been developed based on the analysis of protein substances by which adulteration is detected, they have low efficiency in the case of processed products. In this context, methods based on DNA analysis have become the best choice, although the results are not universally valid and must be adapted to the composition and local specificities. Here is presented a study of the applicability of a fast and cost-effective DNA-based method to determine the species composition of dairy products by performing a qualitative triplex PCR detection technique in the analysis of local dairy products. The procedure is adapted to the domestic market and considers experimental models developed and well defined internationally. In the in-house validation of this method, three species of ruminants were considered: beef, sheep, and goat. The reference material was represented by cheeses prepared in our laboratory in strictly controlled percentage mixtures. Total genomic DNA isolated from the reference cheeses were used to develop a triplex PCR method, which involves the simultaneous detection of the three species studied using a mixture of three pairs of primers. The reference materials proved to be optimal for such studies and have been used successfully in the process of validating the method of detecting the composition of commercial cheeses.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85399587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0029
F. Lucaci, R. Purdoiu, A. Paștiu, R. Lacatus, Carmen Maria Turcu, Vlad Cocostîrc, D. Pusta
Canine hip dysplasia is characterized by joint instability, subluxation, or even luxation of the femoral head, which causes a deformation of the joint with the early production of coxarthrosis. The main purpose of the paper was to determine the incidence of canine hip dysplasia from October 2019 until June 2021 in the Faculty of Veterinary Medicine in Cluj-Napoca. A total of 173 X-rays on the canine hips were taken between October 2019 and June 2021 in the Radiology Department of the Faculty of Veterinary Medicine from Cluj-Napoca. X-rays were taken with the Roentgen TEMCO Grx machine, then imported into the RadiAnt DICOM™ program, and interpreted according to the FCI grading scheme. A total number of 88 X-rays were excluded due to different causes, thus our study presents 85 X-ray images of the hip from different breeds of dogs. From a total of 85 dogs presented in this study, 38 dogs were graded A, 10 dogs were graded B, 22 dogs were classified with grade C, with grade D were classified 6 dogs and with grade E were diagnosed 9 dogs. Out of 85 dogs examined (49 males and 36 females) more than half (N=48; 56,5%) were free from canine hip dysplasia.
{"title":"Incidence of Canine Hip Dysplasia in Radiology Department of the Faculty of Veterinary Medicine Cluj-Napoca (October 2019 - June 2021)","authors":"F. Lucaci, R. Purdoiu, A. Paștiu, R. Lacatus, Carmen Maria Turcu, Vlad Cocostîrc, D. Pusta","doi":"10.15835/buasvmcn-vm:2021.0029","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0029","url":null,"abstract":"Canine hip dysplasia is characterized by joint instability, subluxation, or even luxation of the femoral head, which causes a deformation of the joint with the early production of coxarthrosis. The main purpose of the paper was to determine the incidence of canine hip dysplasia from October 2019 until June 2021 in the Faculty of Veterinary Medicine in Cluj-Napoca. A total of 173 X-rays on the canine hips were taken between October 2019 and June 2021 in the Radiology Department of the Faculty of Veterinary Medicine from Cluj-Napoca. X-rays were taken with the Roentgen TEMCO Grx machine, then imported into the RadiAnt DICOM™ program, and interpreted according to the FCI grading scheme. A total number of 88 X-rays were excluded due to different causes, thus our study presents 85 X-ray images of the hip from different breeds of dogs. From a total of 85 dogs presented in this study, 38 dogs were graded A, 10 dogs were graded B, 22 dogs were classified with grade C, with grade D were classified 6 dogs and with grade E were diagnosed 9 dogs. Out of 85 dogs examined (49 males and 36 females) more than half (N=48; 56,5%) were free from canine hip dysplasia.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82389002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-14DOI: 10.15835/buasvmcn-vm:2021.0030
A. M. ZAGRAI (MAIEREAN), G. Cotor, G. Zagrai, G. Gâjâilă, M. Ghita, A. Ionescu, D. Cotor, A. Damian
Animal stress is a current topic intensely addressed. The general adaptation syndrome, following short exposure to stressors with low or medium intensity, has a positive effect, inducing eustress and improving adaptive capacity. Exposure to strong stressors or over a long period of time has the effect of inducing distress. The aim was to evaluate the stressful effect they have on horses, the specific activities of equine-assisted therapy, in the case of children with autism spectrum disorders. Were used 10 riding horses (8 females and 2 males), of purebred English breed, divided into 2 groups, aged between 4-8 years. The animals had the same maintenance conditions and lifestyle. Samples were taken from the jugular vein in 5 ml heparinized tubes. The parameters were determined using a ProCyte Dx Hematology Analyzer. The data obtained were statistically analyzed, calculating the Mean and Standard Deviation (Microsoft Excel). The results show that the specific activities of equine-assisted therapy do not have a stressful effect on the pathways used. These activities were less stressful than regular recreational riding activities in a riding school, subjecting the animal to less intense physical exertion than daily activities.
{"title":"Evaluation of Stress in Riding Horses, During the Horse-Assisted Therapy Activities in the Case of Children with Autism Spectrum Disorders","authors":"A. M. ZAGRAI (MAIEREAN), G. Cotor, G. Zagrai, G. Gâjâilă, M. Ghita, A. Ionescu, D. Cotor, A. Damian","doi":"10.15835/buasvmcn-vm:2021.0030","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2021.0030","url":null,"abstract":"Animal stress is a current topic intensely addressed. The general adaptation syndrome, following short exposure to stressors with low or medium intensity, has a positive effect, inducing eustress and improving adaptive capacity. Exposure to strong stressors or over a long period of time has the effect of inducing distress. The aim was to evaluate the stressful effect they have on horses, the specific activities of equine-assisted therapy, in the case of children with autism spectrum disorders. Were used 10 riding horses (8 females and 2 males), of purebred English breed, divided into 2 groups, aged between 4-8 years. The animals had the same maintenance conditions and lifestyle. Samples were taken from the jugular vein in 5 ml heparinized tubes. The parameters were determined using a ProCyte Dx Hematology Analyzer. The data obtained were statistically analyzed, calculating the Mean and Standard Deviation (Microsoft Excel). The results show that the specific activities of equine-assisted therapy do not have a stressful effect on the pathways used. These activities were less stressful than regular recreational riding activities in a riding school, subjecting the animal to less intense physical exertion than daily activities.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79906200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：