Pub Date : 2021-05-04DOI: 10.15835/BUASVMCN-M:2021.0004
I. Ghiurco, A. Damian, V. Rus, C. Martonoș, M. Matei, V. Buza, L. Ștefănuț, C. Lațiu, I. Vlasiuc, S. Andrei, V. Miclăuș, A. Gal
Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles involved in the production of chemical energy used in the activity of hepatocytes are the mitochondria. The purpose of this study was to verify the mitochondrial load of hepatocytes in all areas of the classical hepatic lobules, in order to indirectly assess the intensity of hepatocyte activity in each area. Materials and Methods Five fresh corpses of chinchilla (Chinchilla lanigera) from an independent breeder from Bistrița-Năsăud county were used. Liver fragments were harvested and fixated in Kolster’s solution for 24 hours, stained with Heidenhain ferric hematoxylin, and assessed using Olympus BX41 microscope. Fixation with Kolster's solution and the staining with Heidenhain's iron hematoxylin clearly shows the hepatocytic mitochondria in shades from gray to black. The liver lobules displayed an uneven distribution of mitochondria depending on the area. In zone 1 of the classical hepatic lobule, the degree of loading of hepatocytes with mitochondria is larger than in zone 2 and much larger than in zone 3. Morphological features of the hepatocytes, including the number and distribution of mitochondria in the hepatic lobules, should improve the understanding of the physiology and pathology of the liver.
{"title":"Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera)","authors":"I. Ghiurco, A. Damian, V. Rus, C. Martonoș, M. Matei, V. Buza, L. Ștefănuț, C. Lațiu, I. Vlasiuc, S. Andrei, V. Miclăuș, A. Gal","doi":"10.15835/BUASVMCN-M:2021.0004","DOIUrl":"https://doi.org/10.15835/BUASVMCN-M:2021.0004","url":null,"abstract":"Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles involved in the production of chemical energy used in the activity of hepatocytes are the mitochondria. The purpose of this study was to verify the mitochondrial load of hepatocytes in all areas of the classical hepatic lobules, in order to indirectly assess the intensity of hepatocyte activity in each area. Materials and Methods Five fresh corpses of chinchilla (Chinchilla lanigera) from an independent breeder from Bistrița-Năsăud county were used. Liver fragments were harvested and fixated in Kolster’s solution for 24 hours, stained with Heidenhain ferric hematoxylin, and assessed using Olympus BX41 microscope. Fixation with Kolster's solution and the staining with Heidenhain's iron hematoxylin clearly shows the hepatocytic mitochondria in shades from gray to black. The liver lobules displayed an uneven distribution of mitochondria depending on the area. In zone 1 of the classical hepatic lobule, the degree of loading of hepatocytes with mitochondria is larger than in zone 2 and much larger than in zone 3. Morphological features of the hepatocytes, including the number and distribution of mitochondria in the hepatic lobules, should improve the understanding of the physiology and pathology of the liver.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74969959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-04DOI: 10.15835/BUASVMCN-VM:2020.0036
A. Neagu, E. Diac, Andrei Răzvan Codea, A. Nagy, A. Oros, A. Dreanca
Veterinary toxicology is a common field in veterinary medicine, being a science closely related to other clinical and para clinical disciplines. The multitude of toxics and the ability of each organism to respond differently to them, nonspecific clinical signs and an inadequate anamnesis can often put the clinician in difficulty when seeking to diagnose. The aim of the study was to highlight the incidence of pet poisoning cases, over a period of 10 years of time at the emergency clinic, faculty of Veterinary Medicine Cluj-Napoca. An epidemiological study was performed with the help of the emergency clinic archive, where the files of the intoxicated patients were selected. The data were centralized in the Microsoft Office Excel. The results obtained revealed the sum of 256 cases of pet poisoning in the last ten years (0.611% of the number of cases registered during this period), of which the predominantly affected species was the dog of mixed breed. Also, within the most common poisonings, the ones with ethylene glycol and anticoagulants rodenticides were observed. In the present study a correlation was established between breed, age, sex and the most frequent incidence of poisoning, common toxins and the connection between the periods of the year.
{"title":"Incidence of Pet Intoxications in the Emergency Clinic, Faculty of Veterinary Medicine Cluj-Napoca","authors":"A. Neagu, E. Diac, Andrei Răzvan Codea, A. Nagy, A. Oros, A. Dreanca","doi":"10.15835/BUASVMCN-VM:2020.0036","DOIUrl":"https://doi.org/10.15835/BUASVMCN-VM:2020.0036","url":null,"abstract":"Veterinary toxicology is a common field in veterinary medicine, being a science closely related to other clinical and para clinical disciplines. The multitude of toxics and the ability of each organism to respond differently to them, nonspecific clinical signs and an inadequate anamnesis can often put the clinician in difficulty when seeking to diagnose. The aim of the study was to highlight the incidence of pet poisoning cases, over a period of 10 years of time at the emergency clinic, faculty of Veterinary Medicine Cluj-Napoca. An epidemiological study was performed with the help of the emergency clinic archive, where the files of the intoxicated patients were selected. The data were centralized in the Microsoft Office Excel. The results obtained revealed the sum of 256 cases of pet poisoning in the last ten years (0.611% of the number of cases registered during this period), of which the predominantly affected species was the dog of mixed breed. Also, within the most common poisonings, the ones with ethylene glycol and anticoagulants rodenticides were observed. In the present study a correlation was established between breed, age, sex and the most frequent incidence of poisoning, common toxins and the connection between the periods of the year.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75178392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0035
T. Marcu, A. Gal, C. Martonoș, V. Rus, A. Damian, V. Miclăuș, A. Mihai
The biologic material used in this study is represented by 6 rabbits, 3 males and 3 females, aged 11 months and having an average weight of 4 kg. After general anaesthesia was performed, 2 mm diameter titanium implants were surgically inserted in the femur. Postoperative care of the animals included daily observation. After 4 weeks the animals were euthanized according to the guidelines. The femurs were collected along with the implants and processed for histological examination. The samples were placed in Stieve mixture for fixation, dehydrated with ethylic alcohol, clarified with 1-Butanol, and followed by paraffin embedding. 5 micrometers sections were cut using a microtome, and then stained using the Goldner trichrome staining method and were examined using an Olympus BX41 microscope with an attached digital camera for image capturing. In all animals, the implant was well tolerated by the bone and soft tissue implantation bed. The osteointegration process took place through bone formation around the implant, with variations in thickness and structure throughout the bone-implant interface. Except for the accelerated speed of osteointegration steps, observed in males, the osteointegration process follows certain stages and no gender related differences. The rapid progress of osteointegration in males resulted in a faster process in this gender compared to females. This aspect is clinically relevant for the appropriate evaluation of waiting periods from implant placement to prosthetic mounting, which according to this study is different in males and females.
{"title":"Comparative Osteointegration Testing of Titanium Implants in Relation to Gender After One Month of Implantation in Rabbit Femur","authors":"T. Marcu, A. Gal, C. Martonoș, V. Rus, A. Damian, V. Miclăuș, A. Mihai","doi":"10.15835/buasvmcn-vm:2020.0035","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0035","url":null,"abstract":"The biologic material used in this study is represented by 6 rabbits, 3 males and 3 females, aged 11 months and having an average weight of 4 kg. After general anaesthesia was performed, 2 mm diameter titanium implants were surgically inserted in the femur. Postoperative care of the animals included daily observation. After 4 weeks the animals were euthanized according to the guidelines. The femurs were collected along with the implants and processed for histological examination. The samples were placed in Stieve mixture for fixation, dehydrated with ethylic alcohol, clarified with 1-Butanol, and followed by paraffin embedding. 5 micrometers sections were cut using a microtome, and then stained using the Goldner trichrome staining method and were examined using an Olympus BX41 microscope with an attached digital camera for image capturing. In all animals, the implant was well tolerated by the bone and soft tissue implantation bed. The osteointegration process took place through bone formation around the implant, with variations in thickness and structure throughout the bone-implant interface. Except for the accelerated speed of osteointegration steps, observed in males, the osteointegration process follows certain stages and no gender related differences. The rapid progress of osteointegration in males resulted in a faster process in this gender compared to females. This aspect is clinically relevant for the appropriate evaluation of waiting periods from implant placement to prosthetic mounting, which according to this study is different in males and females.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81974546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-22DOI: 10.15835/buasvmcn-vm:2020.0030
M. Turcu, L. Bel, Tommaso Collarile, D. Pusta
Most lovebirds (Agapornis spp.) have no distinct sexual dimorphic traits. The objective of the paper was to compare the results of two sexing methods, surgical sexing by celioscopy and DNA sexing by PCR from blood samples, in order to evaluate their effectiveness. Materials used to carry out the studies were a 2.7 mm telescope and endoscopy unit, surgical instruments and DNA sampling kits provided by Exomed.cz laboratory. Forty-two lovebirds were included in this study. The endoscopic sexing procedure was performed by the method described by Divers. Blood sampling for DNA testing by PCR method was performed from the metatarsal vein. The results were different in the case of one lovebird, endoscopic sexing revealed it as male, compared to DNA testing, where it was identified as female. DNA sexing is a non-invasive method that might be more accurate than celioscopy in this species, and bird owners have easier access to it.
{"title":"Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)","authors":"M. Turcu, L. Bel, Tommaso Collarile, D. Pusta","doi":"10.15835/buasvmcn-vm:2020.0030","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2020.0030","url":null,"abstract":"Most lovebirds (Agapornis spp.) have no distinct sexual dimorphic traits. The objective of the paper was to compare the results of two sexing methods, surgical sexing by celioscopy and DNA sexing by PCR from blood samples, in order to evaluate their effectiveness. Materials used to carry out the studies were a 2.7 mm telescope and endoscopy unit, surgical instruments and DNA sampling kits provided by Exomed.cz laboratory. Forty-two lovebirds were included in this study. The endoscopic sexing procedure was performed by the method described by Divers. Blood sampling for DNA testing by PCR method was performed from the metatarsal vein. The results were different in the case of one lovebird, endoscopic sexing revealed it as male, compared to DNA testing, where it was identified as female. DNA sexing is a non-invasive method that might be more accurate than celioscopy in this species, and bird owners have easier access to it.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87263457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0032
V. Buza, M. Matei, L. Ștefănuț
The vertebrate gut has been continuously populated with complex and dynamic microbial and eukaryotic communities, that over millions of years have coevolved both spatially and temporally (Kreisinger et al., 2015). Due to the prolonged coexistence, intestinal parasites (protozoa and helminths) and resident microbiota have developed the ability to influence one another by several mechanisms: 1) produce changes at the level of intestinal mucus and epithelial barrier, 2) alter the host immune response or 3) direct interaction (Leung et al., 2018). The uncontrolled use of anthelmintics can lead to the elimination of commensal organisms and alteration of host immunity and intestinal microbial community composition. Thus, the aim of this research is to highlight the complexity of interactions between intestinal bacteria and parasites and their importance for the host. The “parasitome”- microbiota relationship is a complex phenomenon that plays an essential role in host intestinal homeostasis, the absence or alteration of either of these organisms being able to cause a severe disruption of host immune system (Leung et al., 2018). Is therefore essential to acquire a deeper understanding of the molecular mechanisms of interaction between these two communities.
脊椎动物肠道中不断存在着复杂而动态的微生物和真核生物群落，数百万年来，它们在空间和时间上共同进化(Kreisinger et al.， 2015)。由于长期共存，肠道寄生虫(原生动物和蠕虫)和常驻微生物群已经发展出通过几种机制相互影响的能力:1)在肠道粘液和上皮屏障水平上产生变化，2)改变宿主免疫反应或3)直接相互作用(Leung et al.， 2018)。不加控制地使用驱虫药可导致共生生物的消除和宿主免疫力和肠道微生物群落组成的改变。因此，本研究的目的是强调肠道细菌与寄生虫相互作用的复杂性及其对宿主的重要性。“寄生虫”-微生物群关系是一种复杂的现象，在宿主肠道内稳态中起着至关重要的作用，这些生物中的任何一种的缺失或改变都可能导致宿主免疫系统的严重破坏(Leung et al.， 2018)。因此，有必要对这两个群落之间相互作用的分子机制有更深入的了解。
{"title":"Intestinal Ecosystem: Interaction and Coexistence Between “Parasitome” and Microbial Communities","authors":"V. Buza, M. Matei, L. Ștefănuț","doi":"10.15835/buasvmcn-vm:2019.0032","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0032","url":null,"abstract":"The vertebrate gut has been continuously populated with complex and dynamic microbial and eukaryotic communities, that over millions of years have coevolved both spatially and temporally (Kreisinger et al., 2015). Due to the prolonged coexistence, intestinal parasites (protozoa and helminths) and resident microbiota have developed the ability to influence one another by several mechanisms: 1) produce changes at the level of intestinal mucus and epithelial barrier, 2) alter the host immune response or 3) direct interaction (Leung et al., 2018). The uncontrolled use of anthelmintics can lead to the elimination of commensal organisms and alteration of host immunity and intestinal microbial community composition. Thus, the aim of this research is to highlight the complexity of interactions between intestinal bacteria and parasites and their importance for the host. The “parasitome”- microbiota relationship is a complex phenomenon that plays an essential role in host intestinal homeostasis, the absence or alteration of either of these organisms being able to cause a severe disruption of host immune system (Leung et al., 2018). Is therefore essential to acquire a deeper understanding of the molecular mechanisms of interaction between these two communities.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85910460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0031
Vlad Cocostîrc, D. Pusta
Genomic research of hereditary diseases is highly significant in the development of specific diagnostic markers meant to identify dogs carrying gene mutations. The Online Mendelian Inheritance in Animals database shows that there are 324 Mendelian disorders in dogs, of which 247 have their likely causal gene variation identified. The remaining disorders are candidates for future research in dog and human genetics, as it may lead to the description of the causative genomic variants in both species. This paper aims to briefly describe the techniques currently used in the research of dog genetics. Choosing the proper technique is fundamental, as it may differ in accordance to multiple variables such as transmission pattern, number of individuals taken into study and the quality and quantity of the existing information.
{"title":"Diagnosis of Mendelian Inherited Disorders in Dogs: Brief Literature Review","authors":"Vlad Cocostîrc, D. Pusta","doi":"10.15835/buasvmcn-vm:2019.0031","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0031","url":null,"abstract":"Genomic research of hereditary diseases is highly significant in the development of specific diagnostic markers meant to identify dogs carrying gene mutations. The Online Mendelian Inheritance in Animals database shows that there are 324 Mendelian disorders in dogs, of which 247 have their likely causal gene variation identified. The remaining disorders are candidates for future research in dog and human genetics, as it may lead to the description of the causative genomic variants in both species. This paper aims to briefly describe the techniques currently used in the research of dog genetics. Choosing the proper technique is fundamental, as it may differ in accordance to multiple variables such as transmission pattern, number of individuals taken into study and the quality and quantity of the existing information.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80782421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0033
J. Savici, D. Brezovan, C. Tulcan, O. Boldura
The aim of this study was the evaluation of integrity biomarkers of reproductive toxicity: histoarchitecture of genital organs (testis and epididymis) and sexual accessory glands (prostate, seminal vesicles and bulbo-urethral glands) at sexual maturity after exposure to potassium dichromate (Cr VI) in suckling period. Consequent to the exposure of male rat pups to hexavalent chromium during suckling period structural changes appeared in genital organs and sexual accessory glands, such as: basal membrane and epithelial disintegration, interstitial edema, wavy basal membrane due to a reduction in tubule diameter, Leydig cell necrosis; epithelial smoothing, epithelial necrosis; epithelial cells desquamation and falling in lumen. Exposure to potassium dichromate (Cr VI) during suckling period produced severe congestive and degenerative lesions in genital organs and sexual accessory glands.
{"title":"The outcome of potassium dichromate exposure on histological structure of male rat sexual organs in suckling period","authors":"J. Savici, D. Brezovan, C. Tulcan, O. Boldura","doi":"10.15835/buasvmcn-vm:2019.0033","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0033","url":null,"abstract":"The aim of this study was the evaluation of integrity biomarkers of reproductive toxicity: histoarchitecture of genital organs (testis and epididymis) and sexual accessory glands (prostate, seminal vesicles and bulbo-urethral glands) at sexual maturity after exposure to potassium dichromate (Cr VI) in suckling period. Consequent to the exposure of male rat pups to hexavalent chromium during suckling period structural changes appeared in genital organs and sexual accessory glands, such as: basal membrane and epithelial disintegration, interstitial edema, wavy basal membrane due to a reduction in tubule diameter, Leydig cell necrosis; epithelial smoothing, epithelial necrosis; epithelial cells desquamation and falling in lumen. Exposure to potassium dichromate (Cr VI) during suckling period produced severe congestive and degenerative lesions in genital organs and sexual accessory glands.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86330831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0030
C. Tulcan, Amandine Horcholle, S. Marc, J. Savici, C. Mircu, I. Huțu, S. Popescu, O. Boldura
The raw milk can support a wide variety of microbiota, due to its high nutritional content. These microorganisms become from a variety of sources and in farm animals and can cause serious damage by infecting the mammary glands and causing mastitis. The mastitic milk is an important source of disease among the consumers. In this paper, we present a study case of pathogenic bacteria identification by DNA based methods from raw milk samples which were collected from farm cows with severe recurrent mastitis. Previously it was diagnosticated that multidrug resistant bacteria E. coli strains are causing those infections. The laboratory procedure consisted of isolation of DNA from raw cow milk samples, followed by PCR based bacterial identification and DNA sequencing. Data collected from sequencing experiment were aligned against reference sequences from bacterial strains. In our study it was found that Pseudomonas aeruginosa, is present in the mastitic milk samples.
{"title":"Bacterian Species Identification from Raw Cow Milk Sample – A Case Study","authors":"C. Tulcan, Amandine Horcholle, S. Marc, J. Savici, C. Mircu, I. Huțu, S. Popescu, O. Boldura","doi":"10.15835/buasvmcn-vm:2019.0030","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0030","url":null,"abstract":"The raw milk can support a wide variety of microbiota, due to its high nutritional content. These microorganisms become from a variety of sources and in farm animals and can cause serious damage by infecting the mammary glands and causing mastitis. The mastitic milk is an important source of disease among the consumers. In this paper, we present a study case of pathogenic bacteria identification by DNA based methods from raw milk samples which were collected from farm cows with severe recurrent mastitis. Previously it was diagnosticated that multidrug resistant bacteria E. coli strains are causing those infections. The laboratory procedure consisted of isolation of DNA from raw cow milk samples, followed by PCR based bacterial identification and DNA sequencing. Data collected from sequencing experiment were aligned against reference sequences from bacterial strains. In our study it was found that Pseudomonas aeruginosa, is present in the mastitic milk samples.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90591090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0038
C. Rîmbu, C. Horhogea, A. Cozma, C. Crețu, M. Grecu, R. Rusu, E. Guguianu
Bite wounds, human and animal origin, can lead to significant complications if appropriate therapy is not undertaken timeously. The major risk of these aggressions is given by the possibility of systemic propagation of the bacteria involved in the wound and the appearance of complications such as osteomyelitis, septic arthritis, bacterial endocarditis, etc. The identification of pathogenic germs and antibiotic sensitivity test were the key points for a specific therapy that could prevent the spread of infection in the deep tissues. Twenty five biological samples collected from various wounds produced by dogs and cats bites over the veterinary personnel during the medical evaluated. Various aerobic pathogenic and conditional pathogenic bacteria (Staphylococcus aureus, Staphylococcus pseudo intermedius, Staphylococcus intermedius, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus xylosus, Staphylococcus warneri, Micrococcus roseus, Streptococcus sp. gr.B, Streptococcus sp. gr.F, Streptococcus sp. gr.G, Enterococcus faecalis, Enterococcus faecium, Trueperella pyogenes, Corynebacterium sp., Bacillus cereus, Pasteurella multocida, Pasteurella canis, Mannheimia haemolitica, Escherichia coli, Pseudomonas aeruginosa, Actinobacillus sp., Neisseria sp) and anaerobic bacteria (Clostridium perfringens, Bacteroides sp.) were isolated. The bacterial drug sensitivity was fluctuating, observing the variations influenced by the natural and acquired resistance of the tested bacterial species. The wounds produced by the bites of dogs and cats have aerobic and anaerobic polymicrobial etiology.
{"title":"Analysis of Bacteriological Infected Dog and Cat Bite Wounds in Veterinary Medical Staff","authors":"C. Rîmbu, C. Horhogea, A. Cozma, C. Crețu, M. Grecu, R. Rusu, E. Guguianu","doi":"10.15835/buasvmcn-vm:2019.0038","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0038","url":null,"abstract":"Bite wounds, human and animal origin, can lead to significant complications if appropriate therapy is not undertaken timeously. The major risk of these aggressions is given by the possibility of systemic propagation of the bacteria involved in the wound and the appearance of complications such as osteomyelitis, septic arthritis, bacterial endocarditis, etc. The identification of pathogenic germs and antibiotic sensitivity test were the key points for a specific therapy that could prevent the spread of infection in the deep tissues. Twenty five biological samples collected from various wounds produced by dogs and cats bites over the veterinary personnel during the medical evaluated. Various aerobic pathogenic and conditional pathogenic bacteria (Staphylococcus aureus, Staphylococcus pseudo intermedius, Staphylococcus intermedius, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus xylosus, Staphylococcus warneri, Micrococcus roseus, Streptococcus sp. gr.B, Streptococcus sp. gr.F, Streptococcus sp. gr.G, Enterococcus faecalis, Enterococcus faecium, Trueperella pyogenes, Corynebacterium sp., Bacillus cereus, Pasteurella multocida, Pasteurella canis, Mannheimia haemolitica, Escherichia coli, Pseudomonas aeruginosa, Actinobacillus sp., Neisseria sp) and anaerobic bacteria (Clostridium perfringens, Bacteroides sp.) were isolated. The bacterial drug sensitivity was fluctuating, observing the variations influenced by the natural and acquired resistance of the tested bacterial species. The wounds produced by the bites of dogs and cats have aerobic and anaerobic polymicrobial etiology.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88867644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-03DOI: 10.15835/buasvmcn-vm:2019.0017
M. Zeghdoudi, Latifa Merdaci, S. Morsli, M. Tahri, L. Aoun
The various descriptions reported of necrotic dermatitis and its pathogenesis remains ambiguous. The purpose of this study is to suggest some pathognomonic characters of the disease with effective preventive and curative measures. Dead birds presented sudden death, greenish lesions at the wings and abdomen with adjacent tissues destruction. The distribution of lesions on 103 dead birds at 45th day of age showed that the right wing and the right abdomen are the most affected. The disease caused a mortality average rate of 12,8 % in a period of 5 days and treatments based on several antibiotics showed that only ampicillin and oxytetracycline gave positive results. The use of API 20 A kit confirmed the presence of Clostridium septicum. The present study showed that necrotic dermatitis must be differenced from others skin affections. Qualities of litter, pathological diagnosis, rapid and judicious treatment, are the most effective means to control this disease.
{"title":"Bacteriological Study and Pathological Features of Necrotic Dermatitis in Broilers in Eastern Algeria","authors":"M. Zeghdoudi, Latifa Merdaci, S. Morsli, M. Tahri, L. Aoun","doi":"10.15835/buasvmcn-vm:2019.0017","DOIUrl":"https://doi.org/10.15835/buasvmcn-vm:2019.0017","url":null,"abstract":"The various descriptions reported of necrotic dermatitis and its pathogenesis remains ambiguous. The purpose of this study is to suggest some pathognomonic characters of the disease with effective preventive and curative measures. Dead birds presented sudden death, greenish lesions at the wings and abdomen with adjacent tissues destruction. The distribution of lesions on 103 dead birds at 45th day of age showed that the right wing and the right abdomen are the most affected. The disease caused a mortality average rate of 12,8 % in a period of 5 days and treatments based on several antibiotics showed that only ampicillin and oxytetracycline gave positive results. The use of API 20 A kit confirmed the presence of Clostridium septicum. The present study showed that necrotic dermatitis must be differenced from others skin affections. Qualities of litter, pathological diagnosis, rapid and judicious treatment, are the most effective means to control this disease.","PeriodicalId":9470,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89451047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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