Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.001
Gaojun Lu, Ruo-tian Wang, X. Tian, Xin Jin
Objective To investigate the value of the folate receptor (FR)-positive circulating tumor cell (CTC) detection in the diagnosis of benign and malignant subcentimeter pulmonary nodules(the maximum diameter ≤10 mm). Methods Thirty-seven patients with subcentimeter pulmonary nodules (the chest CT showed the maximum diameter was ≤10 mm) in the Xuanwu Hospital of Capital Medical University from July to December 2018 were collected. Among them, 22 cases were diagnosed with early stage lung adenocarcinoma by postoperative pathological diagnosis and another 15 cases were benign lung lesion. Venous blood samples from these patients were collected before surgery and then utilized to detect FR+ CTC level (defined unit as FU/3 ml) by novel ligand-targeted polymerase chain reaction (LT-PCR), and the enzyme-linked immunosorbent assay was used to detect the levels of tumor markers, including carcinoembryonic antigen (CEA), neuron-specific enolase(NSE), cytokeratin 19 fragment CYFRA21-1, carbohydrate antigen 125 (CA125), CA199, pro-gastrin releasing peptide (pro-GRP), etc. The t-test was used to compare the measurement values between the groups. The CTC value 8.70 FU/3 ml described in the detection kit instruction was used as the threshold. The binary logistic regression was used to analyze the risk factors of malignant pulmonary nodules. The kappa consistency test was used to identify the consistency of the diagnosis results obtained by the FR+ CTC level and the pathological results of surgically resected specimens. The receiver operating characteristic curve (ROC) was drawn to evaluate the efficiency of each index for the diagnosis of benign and malignant subcentimeter pulmonary nodules. Results The level of FR+ CTC in patients with early stage lung cancer was higher than that in patients with benign lung lesion, and the difference was statistically significant [(11.0±3.0) FU/3 ml vs. (7.0±3.7) FU/3 ml, t=-3.327, P = 0.001]. The level of FR+ CTC was not related to the age, gender and smoking history of patients (all P>0.05). Logistic regression analysis indicated that high-level FR+ CTC was one of the risk factors for malignant pulmonary nodules (OR = 37.333, 95% CI 3.994-349.010, P = 0.002). The kappa consistency test indicated that the level of FR+ CTC used for the diagnosis of lung subcentimeter nodules presented a certain accuracy (κ = 0.627, P < 0.01). ROC illustrated that the FR+ CTC was better than CEA, NSE and CYFRA21-1 when it was used as an indicator for the diagnosis of malignant pulmonary nodules. The area under the curve(AUC) of FR+ CTC was 0.830 (95% CI 0.639-0.968), and the diagnostic sensitivity and specificity were 72.7% (95% CI 49.6%-88.4%) and 93.3% (95% CI 66.0%-99.7%), respectively. When FR+ CTC, CEA, NSE and CYFRA21-1 were combined for lung cancer diagnosis, the AUC, sensitivity and specificity were 0.776 (95% CI 0.614-0.938), 86.4% and 73.3%, respectively. Conclusion The detection of FR+ CTC has a high value in the dia
{"title":"Diagnostic value of folate receptor-positive circulating tumor cell detection in subcentimeter pulmonary nodules","authors":"Gaojun Lu, Ruo-tian Wang, X. Tian, Xin Jin","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.001","url":null,"abstract":"Objective \u0000To investigate the value of the folate receptor (FR)-positive circulating tumor cell (CTC) detection in the diagnosis of benign and malignant subcentimeter pulmonary nodules(the maximum diameter ≤10 mm). \u0000 \u0000 \u0000Methods \u0000Thirty-seven patients with subcentimeter pulmonary nodules (the chest CT showed the maximum diameter was ≤10 mm) in the Xuanwu Hospital of Capital Medical University from July to December 2018 were collected. Among them, 22 cases were diagnosed with early stage lung adenocarcinoma by postoperative pathological diagnosis and another 15 cases were benign lung lesion. Venous blood samples from these patients were collected before surgery and then utilized to detect FR+ CTC level (defined unit as FU/3 ml) by novel ligand-targeted polymerase chain reaction (LT-PCR), and the enzyme-linked immunosorbent assay was used to detect the levels of tumor markers, including carcinoembryonic antigen (CEA), neuron-specific enolase(NSE), cytokeratin 19 fragment CYFRA21-1, carbohydrate antigen 125 (CA125), CA199, pro-gastrin releasing peptide (pro-GRP), etc. The t-test was used to compare the measurement values between the groups. The CTC value 8.70 FU/3 ml described in the detection kit instruction was used as the threshold. The binary logistic regression was used to analyze the risk factors of malignant pulmonary nodules. The kappa consistency test was used to identify the consistency of the diagnosis results obtained by the FR+ CTC level and the pathological results of surgically resected specimens. The receiver operating characteristic curve (ROC) was drawn to evaluate the efficiency of each index for the diagnosis of benign and malignant subcentimeter pulmonary nodules. \u0000 \u0000 \u0000Results \u0000The level of FR+ CTC in patients with early stage lung cancer was higher than that in patients with benign lung lesion, and the difference was statistically significant [(11.0±3.0) FU/3 ml vs. (7.0±3.7) FU/3 ml, t=-3.327, P = 0.001]. The level of FR+ CTC was not related to the age, gender and smoking history of patients (all P>0.05). Logistic regression analysis indicated that high-level FR+ CTC was one of the risk factors for malignant pulmonary nodules (OR = 37.333, 95% CI 3.994-349.010, P = 0.002). The kappa consistency test indicated that the level of FR+ CTC used for the diagnosis of lung subcentimeter nodules presented a certain accuracy (κ = 0.627, P < 0.01). ROC illustrated that the FR+ CTC was better than CEA, NSE and CYFRA21-1 when it was used as an indicator for the diagnosis of malignant pulmonary nodules. The area under the curve(AUC) of FR+ CTC was 0.830 (95% CI 0.639-0.968), and the diagnostic sensitivity and specificity were 72.7% (95% CI 49.6%-88.4%) and 93.3% (95% CI 66.0%-99.7%), respectively. When FR+ CTC, CEA, NSE and CYFRA21-1 were combined for lung cancer diagnosis, the AUC, sensitivity and specificity were 0.776 (95% CI 0.614-0.938), 86.4% and 73.3%, respectively. \u0000 \u0000 \u0000Conclusion \u0000The detection of FR+ CTC has a high value in the dia","PeriodicalId":9505,"journal":{"name":"肿瘤研究与临床","volume":"32 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43811463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.003
Xiao-yan Wang, T. Xing, G. Han, Yi Liu, Xiaoli An
Objective To study the effects of different anesthesia depths on stress response during single-lung ventilation in patients with thoracoscopic lobectomy. Methods Sixty patients selected for elective thoracoscopic lobectomy in the Second Hospital of Shanxi Medical University from September 2018 to May 2019 were randomly divided into three groups according to the digital random table method, with 20 patients in each group. Group A maintained deep anesthesia with the bispectral index (BIS) 36-45, group B maintained moderate anesthesia with BIS 46-55, and group C did not undergo BIS monitoring. The changes of heart rate, mean arterial pressure (MAP), stress indexes cortisol and blood glucose before anesthesia induction (T0), immediately after one-lung ventilation (T1), 60 min after one-lung ventilation (T2) and immediately after skin suture (T3) in the three groups were compared. Results The concentration of blood glucose in group A at T1, T2 and T3 was (5.28±0.49) mmol/L, (5.34±0.49) mmol/L and (5.40±0.47) mmol/L, and the cortisol was (142.75±31.45) ng/ml, (181.36±19.62) ng/ml and (153.81±33.92) ng/ml; the blood glucose in group B was (5.63±0.35) mmol/L, (6.06±0.19) mmol/L and (5.79±0.44) mmol/L, and the cortisol was (168.45±31.16) ng/ml, (171.09±25.28) ng/ml and (159.39±18.77) ng/ml; the blood glucose in group C was (6.35±0.56) mmol/L, (7.04±0.26) mmol/L and (6.17±0.54) mmol/L, and the cortisol was (191.13±46.00) ng/ml, (283.25±30.07) ng/ml and (183.01±19.71) ng/ml, respectively. The blood glucose and cortisol levels in group C at T1, T2 and T3 were higher than those in group A and group B (all P < 0.05). The MAP in group A at T1, T2 and T3 were (69±5) mmHg (1 mmHg= 0.133 kPa), (67±6) mmHg and (75±7) mmHg, respectively, and group B was (80±8) mmHg, (79±4) mmHg and (84±9) mmHg, the differences between the two groups were statistically significant (all P < 0.05). There was significant difference in cortisol between group A and group B at T1 (P < 0.05). The heart rate and MAP at T1, T2 and T3 in group A and group C were significantly different from those at T0 (all P < 0.05). The heart rate and MAP at T1 and T2 in groups B were significantly different from those at T0 (all P < 0.05). Conclusion BIS anesthesia depth monitoring should be performed during single-lung ventilation in thoracic surgery, and BIS should be maintained at 46-55, which can not only inhibit the stress response but also have a slight effect on hemodynamics. Key words: Surgical procedures, operative; Monitoriry, intraoperative; Stress; Depth of anesthesia; Bispectral index monitoring
{"title":"Effect of anesthesia depth on stress response during single-lung ventilation in thoracic surgery","authors":"Xiao-yan Wang, T. Xing, G. Han, Yi Liu, Xiaoli An","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.003","url":null,"abstract":"Objective \u0000To study the effects of different anesthesia depths on stress response during single-lung ventilation in patients with thoracoscopic lobectomy. \u0000 \u0000 \u0000Methods \u0000Sixty patients selected for elective thoracoscopic lobectomy in the Second Hospital of Shanxi Medical University from September 2018 to May 2019 were randomly divided into three groups according to the digital random table method, with 20 patients in each group. Group A maintained deep anesthesia with the bispectral index (BIS) 36-45, group B maintained moderate anesthesia with BIS 46-55, and group C did not undergo BIS monitoring. The changes of heart rate, mean arterial pressure (MAP), stress indexes cortisol and blood glucose before anesthesia induction (T0), immediately after one-lung ventilation (T1), 60 min after one-lung ventilation (T2) and immediately after skin suture (T3) in the three groups were compared. \u0000 \u0000 \u0000Results \u0000The concentration of blood glucose in group A at T1, T2 and T3 was (5.28±0.49) mmol/L, (5.34±0.49) mmol/L and (5.40±0.47) mmol/L, and the cortisol was (142.75±31.45) ng/ml, (181.36±19.62) ng/ml and (153.81±33.92) ng/ml; the blood glucose in group B was (5.63±0.35) mmol/L, (6.06±0.19) mmol/L and (5.79±0.44) mmol/L, and the cortisol was (168.45±31.16) ng/ml, (171.09±25.28) ng/ml and (159.39±18.77) ng/ml; the blood glucose in group C was (6.35±0.56) mmol/L, (7.04±0.26) mmol/L and (6.17±0.54) mmol/L, and the cortisol was (191.13±46.00) ng/ml, (283.25±30.07) ng/ml and (183.01±19.71) ng/ml, respectively. The blood glucose and cortisol levels in group C at T1, T2 and T3 were higher than those in group A and group B (all P < 0.05). The MAP in group A at T1, T2 and T3 were (69±5) mmHg (1 mmHg= 0.133 kPa), (67±6) mmHg and (75±7) mmHg, respectively, and group B was (80±8) mmHg, (79±4) mmHg and (84±9) mmHg, the differences between the two groups were statistically significant (all P < 0.05). There was significant difference in cortisol between group A and group B at T1 (P < 0.05). The heart rate and MAP at T1, T2 and T3 in group A and group C were significantly different from those at T0 (all P < 0.05). The heart rate and MAP at T1 and T2 in groups B were significantly different from those at T0 (all P < 0.05). \u0000 \u0000 \u0000Conclusion \u0000BIS anesthesia depth monitoring should be performed during single-lung ventilation in thoracic surgery, and BIS should be maintained at 46-55, which can not only inhibit the stress response but also have a slight effect on hemodynamics. \u0000 \u0000 \u0000Key words: \u0000Surgical procedures, operative; Monitoriry, intraoperative; Stress; Depth of anesthesia; Bispectral index monitoring","PeriodicalId":9505,"journal":{"name":"肿瘤研究与临床","volume":"32 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47321753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Neoadjuvant chemotherapy combined with R0 resection for treatment of pulmonary sarcomatoid carcinoma: report of one case and review of literature","authors":"Shu-dong Yang, Weifei Fan","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.012","url":null,"abstract":"目的 \u0000探讨肺肉瘤样癌（PSC）新辅助化疗对患者手术的影响。 \u0000 \u0000 \u0000方法 \u0000回顾性分析南京医科大学附属老年医院收治的1例PSC（cT3NxM0）患者的临床资料、病理特点和治疗方案，并复习相关文献。 \u0000 \u0000 \u0000结果 \u0000该患者接受2个周期新辅助化疗后，肺部原发灶退缩明显，获得手术切除机会，实现R0切除。术后病理分期为ypT1N0M0 ⅠA期。 \u0000 \u0000 \u0000结论 \u0000PSC预后差，手术切除是最重要的治疗模式。早期患者建议采用新辅助化疗联合手术切除，或手术切除联合辅助化疗方式治疗。","PeriodicalId":9505,"journal":{"name":"Cancer Research and Clinic","volume":"32 1","pages":"55-57"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44118506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.004
Yunkui Zhang, R. Zhang, S. Peng
Objective To explore the value of the constructed prognostic prediction model of resectable lung cancer in predicting the survival and prognosis of patients. Methods A total of 2 267 patients with primary lung cancer in Shanxi Provincial Cancer Hospital from January 2007 to September 2018 were selected. All patients underwent primary lung cancer surgery without a second primary tumor. Gender, age, occupation, tumor site, pathological type, surgical path, surgical method, tumor stage and treatment were selected as the prognostic factors. A Cox proportional hazard model was used to construct a prognostic index (PI) equation to calculate the PI value of each patient. According to the different ranges of PI values, the low-, intermediate- and high-risk prognosis groups were divided, and the survival status of three groups were evaluated. Results Gender (RR= 0.684, P= 0.001), age (RR= 0.591, P -0.5 was the high-risk group, and the differences of their survival rates were statistically significant (P < 0.05). The 1-, 3-, and 5-year survival rates for the low-, risk groups were 96.8%, 87.0% and 77.9%; the intermediate-risk group were 91.8%, 82.2% and 61.7%; the high-risk group were 86.5%, 61.7% and 50.3%. respectively. Conclusion The prognostic prediction model of resectable lung cancer can predict the prognosis risk and the corresponding survival rate of patients with resectable lung cancer, and it can help clinicians to evaluate the prognosis and formulate subsequent treatment plans. Key words: Lung neoplasms; Thoracic surgical procedures; Prognosis; Models, genetic; Survival analysis
目的探讨构建的可切除肺癌预后预测模型在预测患者生存及预后方面的价值。方法选取2007年1月至2018年9月山西省肿瘤医院原发性肺癌患者2 267例。所有患者均行无第二原发肿瘤的原发性肺癌手术。选择性别、年龄、职业、肿瘤部位、病理类型、手术路径、手术方式、肿瘤分期及治疗作为影响预后的因素。采用Cox比例风险模型构建预后指数(PI)方程，计算每位患者的PI值。根据PI值的不同范围分为低、中、高危预后组，评估三组患者的生存状况。结果性别(RR= 0.684, P= 0.001)、年龄(RR= 0.591, P -0.5)为高危组，其生存率差异有统计学意义(P < 0.05)。低危组的1、3、5年生存率分别为96.8%、87.0%和77.9%;中危组分别为91.8%、82.2%和61.7%;高危组分别为86.5%、61.7%和50.3%。分别。结论可切除肺癌预后预测模型可预测可切除肺癌患者的预后风险及相应的生存率，可帮助临床医生评估预后并制定后续治疗方案。关键词:肺肿瘤;胸外科手术;预后;遗传模型;生存分析
{"title":"Construction of prognostic prediction model of resectable lung cancer and survival analysis","authors":"Yunkui Zhang, R. Zhang, S. Peng","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.004","url":null,"abstract":"Objective \u0000To explore the value of the constructed prognostic prediction model of resectable lung cancer in predicting the survival and prognosis of patients. \u0000 \u0000 \u0000Methods \u0000A total of 2 267 patients with primary lung cancer in Shanxi Provincial Cancer Hospital from January 2007 to September 2018 were selected. All patients underwent primary lung cancer surgery without a second primary tumor. Gender, age, occupation, tumor site, pathological type, surgical path, surgical method, tumor stage and treatment were selected as the prognostic factors. A Cox proportional hazard model was used to construct a prognostic index (PI) equation to calculate the PI value of each patient. According to the different ranges of PI values, the low-, intermediate- and high-risk prognosis groups were divided, and the survival status of three groups were evaluated. \u0000 \u0000 \u0000Results \u0000Gender (RR= 0.684, P= 0.001), age (RR= 0.591, P -0.5 was the high-risk group, and the differences of their survival rates were statistically significant (P < 0.05). The 1-, 3-, and 5-year survival rates for the low-, risk groups were 96.8%, 87.0% and 77.9%; the intermediate-risk group were 91.8%, 82.2% and 61.7%; the high-risk group were 86.5%, 61.7% and 50.3%. respectively. \u0000 \u0000 \u0000Conclusion \u0000The prognostic prediction model of resectable lung cancer can predict the prognosis risk and the corresponding survival rate of patients with resectable lung cancer, and it can help clinicians to evaluate the prognosis and formulate subsequent treatment plans. \u0000 \u0000 \u0000Key words: \u0000Lung neoplasms; Thoracic surgical procedures; Prognosis; Models, genetic; Survival analysis","PeriodicalId":9505,"journal":{"name":"Cancer Research and Clinic","volume":"32 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43567644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.008
Yanian Zhang, Wentao Dong, Wenlu Xiao
Objective To explore the characteristics and significances of gene mutations in pulmonary adenocarcinoma, and to provide evidence for targeted medication. Methods High throughput sequencing based target-capture sequencing was performed in 104 patients with pulmonary adenocarcinoma to detect the mutational status of 56 cancer-related genes. All patients were diagnosed in the First People's Hospital of Kunshan from May 2017 to August 2018. The mutational characteristics of pulmonary adenocarcinoma was analyzed and compared with European and American pulmonary adenocarcinoma populations. The correlations between mutational characteristics and clinical features were analyzed, and the mutation sites for targeted medication were screened. Results Among 104 patients with pulmonary adenocarcinoma, totally 34 mutational genes were detected in 84 patients (81%, 84/104). Highly frequent mutations included epidermal growth factor receptor (EGFR) (49%, 51/104), TP53 (21%, 22/104), KRAS (13%, 14/104), and BRAF (6%, 6/104). Among all the 187 variants, 76% (142/187) were non-synonymous missense mutations, 13% (24/187) were small fragment deletions, 6% (12/187) were copy number variants, 3% (5/187) were small fragment insertions, and 2% (4/187) were nonsense site mutations. Among 104 patients with pulmonary adenocarcinoma, 34 targeted drug-associated mutations of 13 genes were detected in 68 patients (65%), and 19 (18%) patients harbored ≥ 2 targeted drug-associated mutations. EGFR mutations were more common in female patients than in male patients [62% (34/55)vs. 35% (17/49), χ2= 7.629, P= 0.006], while KRAS mutations were more frequent in male patients than in female patients [22% (11/49) vs. 5% (3/55), χ2= 6.424, P= 0.011]. The mutation frequencies of gene EGFR, TP53, KRAS, and CDKN2A in Chinese single-center (the First People's Hospital of Kunshan) and European and American adenocarcinoma populations were significantly different (all P < 0.05). Conclusions The molecular mutational characteristics of pulmonary adenocarcinoma are complex, and vary greatly among different populations. High throughput sequencing-based multiple-gene detection can reveal its mutational features comprehensively, and that has important roles in personal targeted medication guidance, drug-resistance monitoring and prognosis evaluation. Key words: Lung neoplasms; Adenocarcinoma; High-throughput nucleotide sequencing; Mutation; Molecular targeted therapy
{"title":"Characteristics of gene mutations in Chinese single-center pulmonary adenocarcinoma patients and comparison with European and American pulmonary adenocarcinoma populations","authors":"Yanian Zhang, Wentao Dong, Wenlu Xiao","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.008","url":null,"abstract":"Objective \u0000To explore the characteristics and significances of gene mutations in pulmonary adenocarcinoma, and to provide evidence for targeted medication. \u0000 \u0000 \u0000Methods \u0000High throughput sequencing based target-capture sequencing was performed in 104 patients with pulmonary adenocarcinoma to detect the mutational status of 56 cancer-related genes. All patients were diagnosed in the First People's Hospital of Kunshan from May 2017 to August 2018. The mutational characteristics of pulmonary adenocarcinoma was analyzed and compared with European and American pulmonary adenocarcinoma populations. The correlations between mutational characteristics and clinical features were analyzed, and the mutation sites for targeted medication were screened. \u0000 \u0000 \u0000Results \u0000Among 104 patients with pulmonary adenocarcinoma, totally 34 mutational genes were detected in 84 patients (81%, 84/104). Highly frequent mutations included epidermal growth factor receptor (EGFR) (49%, 51/104), TP53 (21%, 22/104), KRAS (13%, 14/104), and BRAF (6%, 6/104). Among all the 187 variants, 76% (142/187) were non-synonymous missense mutations, 13% (24/187) were small fragment deletions, 6% (12/187) were copy number variants, 3% (5/187) were small fragment insertions, and 2% (4/187) were nonsense site mutations. Among 104 patients with pulmonary adenocarcinoma, 34 targeted drug-associated mutations of 13 genes were detected in 68 patients (65%), and 19 (18%) patients harbored ≥ 2 targeted drug-associated mutations. EGFR mutations were more common in female patients than in male patients [62% (34/55)vs. 35% (17/49), χ2= 7.629, P= 0.006], while KRAS mutations were more frequent in male patients than in female patients [22% (11/49) vs. 5% (3/55), χ2= 6.424, P= 0.011]. The mutation frequencies of gene EGFR, TP53, KRAS, and CDKN2A in Chinese single-center (the First People's Hospital of Kunshan) and European and American adenocarcinoma populations were significantly different (all P < 0.05). \u0000 \u0000 \u0000Conclusions \u0000The molecular mutational characteristics of pulmonary adenocarcinoma are complex, and vary greatly among different populations. High throughput sequencing-based multiple-gene detection can reveal its mutational features comprehensively, and that has important roles in personal targeted medication guidance, drug-resistance monitoring and prognosis evaluation. \u0000 \u0000 \u0000Key words: \u0000Lung neoplasms; Adenocarcinoma; High-throughput nucleotide sequencing; Mutation; Molecular targeted therapy","PeriodicalId":9505,"journal":{"name":"肿瘤研究与临床","volume":"32 1","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48038388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.016
Jinke Ding, Chenglong Li, H. Wang
Pituitary adenoma is a common neuroendocrine tumor, and its complex pathogenesis makes its treatment a clinical problem. Recently, studies have shown that long non-coding RNA (LncRNA), as a class of endogenous RNA with a length of more than 200 nucleotides and basically no protein-coding function, plays an important regulatory role in various forms in multiple tumors including pituitary adenoma, and is closely related to the proliferation, invasion and metastasis of pituitary adenoma. Based on the latest research status at home and abroad, this paper summarizes the research progress of LncRNA in pituitary adenoma, hoping to provide new ideas for the diagnosis, treatment and prognosis of pituitary adenoma. Key words: Neuroendocrine tumors; Brain neoplasms; Long non-coding RNA
{"title":"Progress of long non-coding RNA in pituitary adenoma","authors":"Jinke Ding, Chenglong Li, H. Wang","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.016","url":null,"abstract":"Pituitary adenoma is a common neuroendocrine tumor, and its complex pathogenesis makes its treatment a clinical problem. Recently, studies have shown that long non-coding RNA (LncRNA), as a class of endogenous RNA with a length of more than 200 nucleotides and basically no protein-coding function, plays an important regulatory role in various forms in multiple tumors including pituitary adenoma, and is closely related to the proliferation, invasion and metastasis of pituitary adenoma. Based on the latest research status at home and abroad, this paper summarizes the research progress of LncRNA in pituitary adenoma, hoping to provide new ideas for the diagnosis, treatment and prognosis of pituitary adenoma. \u0000 \u0000 \u0000Key words: \u0000Neuroendocrine tumors; Brain neoplasms; Long non-coding RNA","PeriodicalId":9505,"journal":{"name":"肿瘤研究与临床","volume":"32 1","pages":"69-72"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46561984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To compare the effects of epidural anesthesia and paravertebral nerve block on non tracheal intubation single hole thoracoscopic surgery. Method: A total of 45 patients with benign and malignant lung tumors who underwent non tracheal intubation anesthesia single hole thoracoscopic surgery and were admitted to the First Affiliated Hospital of Jinzhou Medical University from November 2017 to June 2019 were randomly divided into a paravertebral nerve block group (22 cases) and an epidural anesthesia group (23 cases) using a random number table method. Before induction of anesthesia, the paravertebral nerve block group was guided by ultrasound to block the thoracic 3-6 spinous nerve roots; The epidural anesthesia group underwent epidural puncture and catheterization anesthesia in the 6-7 or 7-8 intervals of the chest. Compare the surgical time, intraoperative bleeding volume, postoperative recovery time of directional force, time of getting out of bed activity, time of catheterization, discharge time, and related complications between the two groups. There was no statistically significant difference in surgical time and intraoperative bleeding between the two groups of patients (both P>0.05). Compared with the epidural anesthesia group, the paraspinal nerve block group had shorter postoperative directional recovery time [(7.22 ± 2.20) minutes compared to (18.80 ± 5.06) minutes], ambulation time [(11.45 ± 3.01) hours compared to (21.90 ± 4.61) hours], tube insertion time [(2.77 ± 0.81) days compared to (5.47 ± 1.31) days], and discharge time [(3.68 ± 0.84) days compared to (6.17 ± 1.03) days], The differences between the two groups were statistically significant (t-values were -9.874, -8.763, -8.282, -7.594, all P<0.05). In the paravertebral nerve block group, there were no postoperative complications such as hypoxemia, hypotension, pulmonary complications, nausea, vomiting, and urinary retention. Conclusion: The use of paravertebral nerve block in non tracheal intubation single hole thoracoscopic surgery has fast recovery and fewer complications, and can be prioritized in clinical practice, which is helpful for rapid recovery.
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1006-9801.2020.01.006
Shuangping Zhang, Shi-ping Guo, Jianhong Lian, Yong Ma
Objective To explore the efficacy of thoracoscopic bronchial sleeve lobectomy for central non-small cell lung cancer (NSCLC), and to evaluate the safety of this operation. Methods The clinical data of 29 patients who underwent thoracoscopic bronchial sleeve lobectomy at Shanxi Provincial Cancer Hospital from May 2015 to September 2018 were retrospectively analyzed, and the surgical effect and safety were analyzed. Results Twenty-nine cases underwent thoracoscopic bronchial sleeve lobectomy. The types of resection included 13 cases of right upper, 10 cases of left upper, and 6 cases of left lower sleeve lobectomy. The operation time was 180-400 min, and the median time was 240 min. The bronchial anastomosis time was 35-60 min, and the median time was 48 min. The intraoperative blood loss was 150-460 ml, and the median blood loss was 220 ml. The number of lymph node dissection was 12-39 lymph nodes per patient, with a median of 19.6 lymph nodes per patient. The thoracic drainage tube was placed for 4-16 days after operation, with a median of 6 days; the postoperative hospital stay was 6-16 days, with a median of 9 days. The postoperative complication rate was 24.1% (7/29), including 1 case with pulmonary air leakage (> 7 days), 2 cases with pulmonary infections, 3 cases with arrhythmia, and 1 patient discharged from the hospital on the 7th day after surgery, but died of anastomotic fistula bleeding on the 40th day. The rest of the patients recovered smoothly after surgery. The median follow-up time was 6 months (3-12 months). No tumor recurrence or anastomotic stenosis was observed. Conclusion Thoracoscopic bronchial sleeve lobectomy is a safe and feasible surgical treatment for central NSCLC. Key words: Carcinoma, non-small-cell lung; Thoracoscopes; Sleeve lobectomy
{"title":"Efficacy and safety of thoracoscopic bronchial sleeve lobectomy for central non-small cell lung cancer","authors":"Shuangping Zhang, Shi-ping Guo, Jianhong Lian, Yong Ma","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.006","url":null,"abstract":"Objective \u0000To explore the efficacy of thoracoscopic bronchial sleeve lobectomy for central non-small cell lung cancer (NSCLC), and to evaluate the safety of this operation. \u0000 \u0000 \u0000Methods \u0000The clinical data of 29 patients who underwent thoracoscopic bronchial sleeve lobectomy at Shanxi Provincial Cancer Hospital from May 2015 to September 2018 were retrospectively analyzed, and the surgical effect and safety were analyzed. \u0000 \u0000 \u0000Results \u0000Twenty-nine cases underwent thoracoscopic bronchial sleeve lobectomy. The types of resection included 13 cases of right upper, 10 cases of left upper, and 6 cases of left lower sleeve lobectomy. The operation time was 180-400 min, and the median time was 240 min. The bronchial anastomosis time was 35-60 min, and the median time was 48 min. The intraoperative blood loss was 150-460 ml, and the median blood loss was 220 ml. The number of lymph node dissection was 12-39 lymph nodes per patient, with a median of 19.6 lymph nodes per patient. The thoracic drainage tube was placed for 4-16 days after operation, with a median of 6 days; the postoperative hospital stay was 6-16 days, with a median of 9 days. The postoperative complication rate was 24.1% (7/29), including 1 case with pulmonary air leakage (> 7 days), 2 cases with pulmonary infections, 3 cases with arrhythmia, and 1 patient discharged from the hospital on the 7th day after surgery, but died of anastomotic fistula bleeding on the 40th day. The rest of the patients recovered smoothly after surgery. The median follow-up time was 6 months (3-12 months). No tumor recurrence or anastomotic stenosis was observed. \u0000 \u0000 \u0000Conclusion \u0000Thoracoscopic bronchial sleeve lobectomy is a safe and feasible surgical treatment for central NSCLC. \u0000 \u0000 \u0000Key words: \u0000Carcinoma, non-small-cell lung; Thoracoscopes; Sleeve lobectomy","PeriodicalId":9505,"journal":{"name":"肿瘤研究与临床","volume":"32 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42715450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To systematically evaluate the efficacy and safety of CT-guided percutaneous lung puncture biopsy versus ultrasound-guided percutaneous lung puncture biopsy. Methods Relevant domestic and foreign related databases such as PubMed, Web of Science, Cochrane Library, OVID, China Biology Medicine, VIP, Wanfang and CNKI databases were searched, the randomized controlled trial about the applications of CT-guided and ultrasound-guided percutaneous lung puncture biopsy were collected. After extracting the relevant data, a Meta-analysis was performed using RevMan 5.2 and Stata softwares. Results Ten studies met the inclusion criteria, with a total sample size of 1 158 cases, of which 635 were CT-guided puncture biopsy and 523 were ultrasound-guided puncture biopsy. Meta-analysis showed that the difference of the success rate between the CT-guided group and the ultrasound-guided group was not statistically significant [97.48% (619/635) vs. 96.56% (505/523), RR= 1.01, 95% CI 0.99-1.03, P= 0.360]; the puncture diagnosis rate in the CT-guided group was lower than in the ultrasound-guided group, and the difference was statistically significant [92.44% (619/635) vs. 96.56% (505/523), RR= 0.97, 95% CI 0.94-0.99, P= 0.007]. The total incidence of complications after puncture in the CT-guided group was higher than that in the ultrasound-guided group, and the difference was statistically significant [14.49% (92/635) vs. 9.56% (50/523), RR= 1.56, 95% CI 1.11-2.19, P= 0.010]; the incidence of pneumothorax after puncture in the CT-guided group was higher than that in the ultrasound-guided group, and the difference was statistically significant [11.50%(73/635) vs. 6.31% (33/523), RR= 1.77, 95% CI 1.17-2.68, P= 0.007]; the difference of the incidence of bleeding after puncture between the CT-guided group and the ultrasound-guided group was not statistically significant [2.99% (19/635) vs. 3.25% (17/523), RR= 1.08, 95% CI 0.59-1.98, P= 0.800]. Conclusions Both CT-guided and ultrasound-guided percutaneous lung puncture biopsy have good clinical diagnostic value. However, for the intrapulmonary masses shown by ultrasound, the ultrasound-guided lung puncture biopsy has a shorter operation time, lower cost, and higher safety. Key words: Biopsy; Punctures; Ultrasonogrophy; Computed tomography; Peripheral pulmonary masses; Meta-analysis
目的系统评价CT引导下经皮肺穿刺活检与超声引导下经皮肺穿刺活检的疗效和安全性。方法检索PubMed、Web of Science、Cochrane Library、OVID、中国生物医药、VIP、万方、CNKI等国内外相关数据库，收集CT引导和超声引导下经皮肺穿刺活检应用的随机对照试验。提取相关数据后，使用RevMan 5.2和Stata软件进行Meta分析。结果10项研究符合纳入标准，总样本量为1588例，其中635例为CT引导下穿刺活检，523例为超声引导下穿刺活组织。荟萃分析显示，CT引导组和超声引导组的成功率差异无统计学意义[97.48%（619/635）vs.96.56%（505/523），RR=1.01，95%CI 0.99-1.03，P=0.360]；CT引导组的穿刺诊断率低于超声引导组，差异有统计学意义[92.44%（619/635）vs.96.56%（505/523），RR=0.97，95%CI 0.94-0.99，P=0.007]。CT引导组穿刺后并发症的总发生率高于超声引导组，差异具有统计学意义[14.49%（92/635）vs.9.56%（50/523），RR=1.56，95%CI1.11-21.19，P=0.010]；CT引导组穿刺后发生率高于超声引导组，差异有统计学意义[11.50%（73/635）vs.6.31%（33/523），RR=1.77，95%CI 1.17-2.68，P=0.007]；CT引导组与超声引导组穿刺后出血发生率差异无统计学意义[2.99%（19/635）vs.3.25%（17/523），RR=1.08，95%CI 0.59-1.98，P=0.800]。然而，对于超声显示的肺内肿块，超声引导下的肺穿刺活检手术时间更短，成本更低，安全性更高。关键词：活检；穿孔；超声造影；计算机断层扫描；周围性肺部肿块；Meta分析
{"title":"CT-guided versus ultrasound-guided percutaneous lung puncture biopsy in the diagnosis of peripheral pulmonary masses: a Meta-analysis","authors":"Meng Hu, Jing Liu, Weirong Yao, Xiquan Zhang, Zhiyong Zhou, Lin Zeng","doi":"10.3760/CMA.J.ISSN.1006-9801.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1006-9801.2020.01.009","url":null,"abstract":"Objective \u0000To systematically evaluate the efficacy and safety of CT-guided percutaneous lung puncture biopsy versus ultrasound-guided percutaneous lung puncture biopsy. \u0000 \u0000 \u0000Methods \u0000Relevant domestic and foreign related databases such as PubMed, Web of Science, Cochrane Library, OVID, China Biology Medicine, VIP, Wanfang and CNKI databases were searched, the randomized controlled trial about the applications of CT-guided and ultrasound-guided percutaneous lung puncture biopsy were collected. After extracting the relevant data, a Meta-analysis was performed using RevMan 5.2 and Stata softwares. \u0000 \u0000 \u0000Results \u0000Ten studies met the inclusion criteria, with a total sample size of 1 158 cases, of which 635 were CT-guided puncture biopsy and 523 were ultrasound-guided puncture biopsy. Meta-analysis showed that the difference of the success rate between the CT-guided group and the ultrasound-guided group was not statistically significant [97.48% (619/635) vs. 96.56% (505/523), RR= 1.01, 95% CI 0.99-1.03, P= 0.360]; the puncture diagnosis rate in the CT-guided group was lower than in the ultrasound-guided group, and the difference was statistically significant [92.44% (619/635) vs. 96.56% (505/523), RR= 0.97, 95% CI 0.94-0.99, P= 0.007]. The total incidence of complications after puncture in the CT-guided group was higher than that in the ultrasound-guided group, and the difference was statistically significant [14.49% (92/635) vs. 9.56% (50/523), RR= 1.56, 95% CI 1.11-2.19, P= 0.010]; the incidence of pneumothorax after puncture in the CT-guided group was higher than that in the ultrasound-guided group, and the difference was statistically significant [11.50%(73/635) vs. 6.31% (33/523), RR= 1.77, 95% CI 1.17-2.68, P= 0.007]; the difference of the incidence of bleeding after puncture between the CT-guided group and the ultrasound-guided group was not statistically significant [2.99% (19/635) vs. 3.25% (17/523), RR= 1.08, 95% CI 0.59-1.98, P= 0.800]. \u0000 \u0000 \u0000Conclusions \u0000Both CT-guided and ultrasound-guided percutaneous lung puncture biopsy have good clinical diagnostic value. However, for the intrapulmonary masses shown by ultrasound, the ultrasound-guided lung puncture biopsy has a shorter operation time, lower cost, and higher safety. \u0000 \u0000 \u0000Key words: \u0000Biopsy; Punctures; Ultrasonogrophy; Computed tomography; Peripheral pulmonary masses; Meta-analysis","PeriodicalId":9505,"journal":{"name":"Cancer Research and Clinic","volume":"32 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48077103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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