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Effects of sugarcane extract on growth and diarrhea of growing piglets 甘蔗提取物对生长仔猪生长发育及腹泻的影响
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-04-12 DOI: 10.1139/cjas-2022-0136
Kai Wang, Xin Ren, X. Shen, Yansen Li, Yangchun Xia, Zhaojian Li, Chunmei Li
Abstract This experiment evaluated the effects of sugarcane extract (SCE) on growing pigs' diarrhea incidence, serum immunity, intestinal morphology, and antioxidant enzyme activity. A total of 116 52-day-old commercial pigs (Duroc × Landrace × Jiaxing Black, average weight of 11 ± 1 kg) were randomly allocated to control (CON, basal diet) and 1% SCE group (SCEG, basal diet + 1% SCE). The experiment lasted four weeks. Compared with CON, diarrhea incidence (SCEG: 74.11% versus CON: 54.76%) and diarrhea index (SCEG: 83.65% versus CON: 73.61%) dropped largely in pigs supplemented with SCE. Villus height in the duodenum (p < 0.01) and the ratio of villus height to crypt depth (p < 0.05) increased in SCEG. Dietary SCE enhanced the activity of catalase (CAT) capacity, and decreased tumor necrosis factor α, and malondialdehyde levels in serum (p < 0.05). CAT activity in the ileum increased (p < 0.05) in piglets supplemented with SCE. Thus, dietary supplementation with SCE improved diarrhea incidence, serum antioxidant capacity and immunity, and intestinal villus morphology and may be used as an efficient antibiotic alternative in weaned piglet feed.
摘要本试验旨在研究甘蔗提取物(SCE)对生长猪腹泻发生率、血清免疫、肠道形态和抗氧化酶活性的影响。试验选用平均体重为11±1 kg的52日龄商品猪(杜×长×嘉兴黑)116头,随机分为对照组(CON,基础日粮)和1% SCE组(SCEG,基础日粮+ 1% SCE)。实验持续了四周。与CON相比,添加SCE的猪腹泻发生率(SCEG: 74.11%, CON: 54.76%)和腹泻指数(SCEG: 83.65%, CON: 73.61%)均显著降低。SCEG组十二指肠绒毛高度(p < 0.01)和绒毛高度与隐窝深度之比(p < 0.05)升高。饲粮SCE提高了血清过氧化氢酶(CAT)活性,降低了血清肿瘤坏死因子α和丙二醛水平(p < 0.05)。添加SCE的仔猪回肠CAT活性显著提高(p < 0.05)。由此可见,饲粮中添加SCE可改善断奶仔猪腹泻发生率、血清抗氧化能力和免疫力,改善肠道绒毛形态,可作为一种有效的抗生素替代品。
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引用次数: 0
Protective effects of methylsulfonylmethane (MSM) on barrier function injury of porcine intestinal epithelial cells (IPEC-J2) induced by lipopolysaccharide (LPS) 甲基磺酰甲烷(MSM)对脂多糖(LPS)诱导的猪肠上皮细胞(IPEC-J2)屏障功能损伤的保护作用
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-04-11 DOI: 10.1139/cjas-2022-0141
Y. Jiao, Hengjiang Li, Ting Ren, I. Kim
Abstract Methylsulfonylmethane (MSM) is a natural organic sulfur component that has anti-inflammatory and antioxidant properties. In this study, injury of porcine intestinal epithelial cell (IPEC-J2) models were used to investigate the effect of MSM on lipopolysaccharide (LPS)-induced porcine intestinal epithelium barrier damage. The results of the cell cycle showed that the cells in the G2/M phase decreased significantly with the supplementation of 300 mmol/L MSM (P < 0.05). The ELISA assay revealed that MSM could significantly inhibit the expression of tumor necrosis factor-alpha, interleukin-1, and interleukin-6 (P < 0.01). Meanwhile, MSM could significantly increase the value of cell monolayer transepithelial electrical resistance while reducing the FITC-dextran flux permeability and lactate dehydrogenase activity in IPEC-J2 cells (P < 0.01). Additionally, 300 mmol/L MSM significantly increased both mRNA and protein expression of occludin, claudin-1, and ZO-1 (P < 0.05). Furthermore, MSM prevented the downregulation of epidermal growth factor receptor (EGFR) by LPS, indicating that MSM might enhance tight junction function through mechanisms of activation of EGFR-mediated protein synthesis in IPEC-J2 cells. Therefore, our findings suggested that MSM has protective effects on inflammation and epithelial barrier injury in LPS-induced IPEC-J2 cells, indicating that MSM might be used as a potential therapeutic agent in the pig industry.
摘要甲基磺酰基甲烷(MSM)是一种具有抗炎和抗氧化特性的天然有机硫成分。本研究采用猪肠上皮细胞(IPEC-J2)损伤模型,探讨MSM对脂多糖(LPS)诱导的猪肠上皮屏障损伤的影响。细胞周期结果显示,添加300 mmol/L MSM后,G2/M期细胞数量显著减少(P < 0.05)。ELISA检测结果显示,MSM能显著抑制肿瘤坏死因子- α、白细胞介素-1、白细胞介素-6的表达(P < 0.01)。MSM能显著提高IPEC-J2细胞单层上皮电阻值,降低fitc -葡聚糖通量通透性和乳酸脱氢酶活性(P < 0.01)。300 mmol/L MSM显著提高了occludin、claudin-1和ZO-1 mRNA和蛋白的表达(P < 0.05)。此外,MSM可以阻止LPS对表皮生长因子受体(EGFR)的下调,表明MSM可能通过激活EGFR介导的蛋白合成机制增强IPEC-J2细胞的紧密连接功能。因此,我们的研究结果表明,MSM对lps诱导的IPEC-J2细胞的炎症和上皮屏障损伤具有保护作用,这表明MSM可能是一种潜在的养猪业治疗剂。
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引用次数: 0
Age-related effects of high-frequency LED lighting in laying hens. Part 1: blood physiological variables 高频LED照明对蛋鸡的年龄相关影响。第一部分:血液生理变量
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-04-06 DOI: 10.1139/cjas-2022-0119
H. Olanrewaju, J. Purswell, J. Evans, S. Collier, S. Branton
Abstract Light impacts layer hen's welfare and production. This study investigated the effects of a novel LED light system on blood physiological variables in laying hens. In each of the two trials, 320-layer pullets were allocated to 16 groups of 20 birds. Treatments included the prototype LED lighting (two rooms) and the conventional 2700 K LED bulbs (two rooms). On each sampling day, blood samples were collected and analyzed immediately. Results show that only a few physiological variables were affected by treatments, but age had effects on most examined variables. No effects of treatments or age on plasma corticosterone signify a similar level of physiological stress.
光照影响蛋鸡的福利和生产。本研究研究了一种新型LED光系统对蛋鸡血液生理指标的影响。在两个试验中,每个试验将320个蛋鸡分配给16组,每组20只鸡。处理包括原型LED照明(两个房间)和传统的2700 K LED灯泡(两个房间)。在每个采样日,立即采集血样并进行分析。结果表明,只有少数生理变量受到治疗的影响,但年龄对大多数被检查的变量有影响。治疗或年龄对血浆皮质酮没有影响,表明生理应激水平相似。
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引用次数: 0
Recording of calf diseases for potential use in breeding programs: a case study on calf respiratory illness and diarrhea 记录小牛疾病以备育种计划之用:小牛呼吸道疾病与腹泻个案研究
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-03-31 DOI: 10.1139/cjas-2022-0112
N. van Staaveren, E. Hyland, K. Houlahan, C. Lynch, F. Miglior, D. Kelton, F. Schenkel, C. Baes
Abstract Calf diseases remain a challenge for dairy producers from both an economic and welfare perspective. Genetically selecting for disease resistance in calves is a promising approach that could contribute to sustainable dairy farming. Genetic evaluations, however, require well-defined and consistently recorded phenotypes to be successful. Therefore, this study aimed to understand the current state of calf disease recording on Ontario farms. Calf disease records of respiratory illness and diarrhea were available from the national milk recording organization (Lactanet Canada, Guelph, Ontario, Canada) from 2009 to 2020. A case study was conducted to describe calf disease diagnoses and recording practices by surveying a subset of 13 Ontario dairy producers. The percentage of milk recorded farms that recorded calf respiratory illness and calf diarrhea increased from 2.6% in 2009 to 11.1% in 2020. Potential sources of data loss were identified along the information chain from farm to genetic evaluation database. Clear definitions and thresholds to diagnose calf disease, standard operating procedures for data recording, as well as a data transfer pipeline, which includes exchange formats, are needed to facilitate the inclusion of calf health traits in genetic evaluations.
从经济和福利的角度来看,小牛疾病仍然是乳品生产者面临的挑战。对小牛进行抗病基因选择是一种很有前途的方法,可能有助于可持续的奶牛养殖。然而,遗传评估需要明确定义和一致记录的表型才能成功。因此,本研究旨在了解安大略省农场小牛疾病记录的现状。从2009年到2020年,可从国家牛奶记录组织(Lactanet Canada, Guelph, Ontario, Canada)获得呼吸道疾病和腹泻的小牛疾病记录。通过调查13个安大略省乳制品生产商的一个子集,进行了一个案例研究,以描述小牛疾病的诊断和记录实践。有小牛呼吸道疾病和腹泻记录的奶场比例从2009年的2.6%上升到2020年的11.1%。在从农场到遗传评估数据库的信息链上确定了潜在的数据丢失来源。诊断小牛疾病的明确定义和阈值、数据记录的标准操作程序以及包括交换格式在内的数据传输管道,都需要有助于将小牛健康特征纳入遗传评估。
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引用次数: 1
Amiata donkey body conformation, udder characteristics, and their relationship with milk yield and quality 毛驴身体形态、乳房特征及其与产奶量和品质的关系
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-03-29 DOI: 10.1139/cjas-2022-0130
F. Salari, I. Altomonte, C. Boselli, M. Martini
Abstract To date, no selective actions have been taken to improve milk traits in dairy donkeys, and the characteristics of the udder are not well defined in relation to the productive characteristics. This study aimed at increasing knowledge on Amiata dairy donkey body conformation, udder traits, and their relationship with milk yield and quality. Morphological, udder, and teat measurements and milk evaluations of 45 pluriparous jennies were carried out. The average wither height of the jennies was 126 cm and the chest girth was 148 cm; a large standard deviation of some body measurements was found. Forty-nine percent of the animals showed a moderately developed udder, while most of the jennies had symmetrical half-udders (96%) and the intermammary cleft was clearly visible in 53% of subjects. Correlation analysis indicated that bigger animals tend to have bigger udders, higher teat diameter, and greater distance between teat tips. A positive correlation between the teat length and the milk fat was found (p < 0.01), which suggests that jennies with longer teats have a better ability to release milk fat. The results of this paper may be useful to define the characteristics of the milking device and address selective choices of the animals.
迄今为止,还没有采取选择性措施来改善乳驴的奶性状,并且与生产特性相关的乳房特征也没有很好地定义。本研究旨在增加对阿米亚塔奶驴身体形态、乳房性状及其与产奶量和品质关系的认识。对45只多产珍妮进行了形态学、乳房和乳头测量和乳汁评价。牛仔裤平均枯高126 cm,胸围148 cm;发现一些身体测量值有很大的标准偏差。49%的动物表现出适度发育的乳房,而大多数珍妮有对称的半乳房(96%),53%的受试者可以清楚地看到乳房间裂。相关分析表明,体型较大的动物往往有较大的乳房、较大的乳头直径和较大的乳头间距。奶头长度与乳脂呈极显著正相关(p < 0.01),说明奶头长度越长,乳脂释放能力越强。本文的研究结果对确定挤奶装置的特点和解决奶牛的选择问题有一定的指导意义。
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引用次数: 0
Note of appreciation 感谢信
4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-03-01 DOI: 10.1139/cjas-2022-0140
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引用次数: 0
Farm size distribution in the Canadian livestock sector 加拿大畜牧业的农场规模分布
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-30 DOI: 10.1139/cjas-2022-0075
A. VanderZaag, S. Bittman, M. Wellisch
Abstract This study quantified the distribution of animals by farm size for portions of the dairy, beef, swine, and poultry sectors using the 2016 Census of Agriculture. Results showed that the distribution of farm sizes and animal populations were skewed or bimodal and varied by livestock type and by province. Thus, the average farm size has little meaning. Recognizing farm size diversity is important for developing effective policies and programs, conducting appropriate research, and developing practices and technologies to support farms of all sizes.
摘要本研究利用2016年农业普查,量化了乳制品、牛肉、猪和家禽部门按农场规模划分的动物分布。结果表明,农场规模和动物种群的分布呈偏态或双峰型,并因牲畜类型和省份而异。因此,平均农场规模没有什么意义。认识到农场规模的多样性对于制定有效的政策和计划、进行适当的研究以及开发支持各种规模农场的实践和技术至关重要。
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引用次数: 0
Thermal processing induced changes on molecular structure spectral profile of carbohydrates and ruminal degradation and intestinal digestion characteristics of oat grains (Avena sativa) in dairy cows: comparison among dry heating vs. moisture heating vs. microwave irradiation 热处理引起的奶牛碳水化合物分子结构光谱谱的变化以及燕麦颗粒(Avena sativa)的瘤胃降解和肠道消化特性:干热与湿热与微波辐射的比较
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-26 DOI: 10.1139/cjas-2022-0061
L. Prates, Carlene Nagy, P. Yu
Abstract In this study, the objectives were to (i) identify the magnitude of differences between dry heating, moisture heating, microwave irradiation, and no-heated treatments on chemical profiles, energy values, the Cornell Net Carbohydrate and Protein System fractions, rumen degradation, and intestinal digestion of newly developed oat (Avena sativa) varieties; (ii) investigate heating induced changes in the molecular structure of the grains with the use of vibrational attenuated total reflectance–Fourier transform infrared (ATR-FTIR) spectroscopy; and (iii) predict rumen degradability of the nutrients based on the molecular spectral profile obtained from ATR-FTIR. Duration and temperature for each processing treatment were established based on previous studies. Results showed that heat processing treatments altered CHO fractions with undegradable CHO fraction (CC) increased by moisture heating. The ATR-FTIR spectroscopy was successful in detecting the processing induced CHO molecular structure changes in oat grain. The CHO molecular profiles were correlated to chemical profile and in situ rumen degradation characteristics. Lastly, multiple regressions with best model variable selection for prediction of nutritional value were obtained. In conclusion, heat processing methods tended to affect both energy values and rumen degradation features. The CHO molecular structure spectral profiles could be used as potential predictors for heated oat grain degradation.
在本研究中,目的是(i)确定干热、湿热、微波辐照和不加热处理在新开发的燕麦品种的化学特征、能量值、康奈尔净碳水化合物和蛋白质系统组分、瘤胃降解和肠道消化方面的差异程度;(ii)利用振动衰减全反射-傅里叶变换红外(ATR-FTIR)光谱研究加热引起的晶粒分子结构变化;(iii)根据ATR-FTIR获得的分子光谱曲线预测营养物质的瘤胃降解率。每个加工处理的持续时间和温度是根据先前的研究确定的。结果表明,热处理改变了CHO组分,不可降解的CHO组分(CC)随着水分加热而增加。ATR-FTIR光谱成功地检测了加工引起的燕麦CHO分子结构变化。CHO分子谱与化学谱和瘤胃原位降解特性相关。最后,用最佳模型变量选择的多元回归方法进行营养价值预测。综上所述,热处理方式对能量值和瘤胃降解特征均有影响。CHO分子结构谱可以作为热降解燕麦籽粒的潜在预测指标。
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引用次数: 0
Effect of quercetin supplementation on growth performance, nutrient digestibility, excreta bacterial count, noxious gas emission, and meat quality of broilers when fed different protein diets 添加槲皮素对不同蛋白质日粮肉鸡生长性能、养分消化率、排泄菌数、有害气体排放和肉质的影响
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-26 DOI: 10.1139/cjas-2021-0122
M. Hoque, I. Kim
Abstract This study aimed to evaluate the effect of flavonoid supplementation to a low-protein diet (LCP) on growth performance, nutrient digestibility, excreta bacterial count, and excreta gas emission of broilers. In total, 800 one-day-old Ross 308 broilers (BW; 42.90±1.43g) were randomly sorted into one of the 4 dietary treatments (10 pens/treatment; 20 birds/pen). Treatment diets were high-protein diet (HCP), basal diet; LCP, basal diet−2.5% protein; TRT1, LCP+0.025% quercetin; and TRT2, LCP+0.050% quercetin. Experimental diets were provided to broilers from days8 to 35. HCP, TRT1, and TRT2 showed higher body weight gain (BWG) than the LCP group during days8–21 and in the overall experiment. However, feed conversion ratio of the HCP group was improved than that of the LCP group during days8–21 and in the overall period. The increasing level of quercetin supplementations brought a linear increase in BWG. Lactobacillus, Escherichia coli, and Salmonella counts in excreta samples of the experimental groups showed no significant difference. Flavonoid supplementation (0.050%) reduced drip loss in breast muscle more than that in the LCP group and showed a linear reduction. Through improved digestion, quercetin addition to an LCP reversed the BWG.
摘要本研究旨在评估在低蛋白日粮中添加黄酮类化合物对肉鸡生长性能、营养物质消化率、排泄菌计数和排泄气体排放的影响。总共将800只一日龄Ross 308肉鸡(BW;42.90±1.43g)随机分为4个日粮处理之一(10个围栏/处理;20只鸟/围栏)。治疗日粮为高蛋白日粮(HCP)、基础日粮;LCP,基础饮食−2.5%蛋白质;TRT1、LCP+0.025%槲皮素;TRT2、LCP+0.050%槲皮素。试验日粮从第8天至第35天提供给肉鸡。HCP、TRT1和TRT2在第8-21天和整个实验中显示出比LCP组更高的体重增加(BWG)。然而,在第8-21天和整个期间,HCP组的饲料转化率比LCP组有所提高。槲皮素补充水平的增加使BWG呈线性增加。实验组排泄物样本中的乳酸杆菌、大肠杆菌和沙门氏菌计数无显著差异。与无导线心脏起搏器组相比,补充类黄酮(0.050%)可减少胸肌的滴水量,并呈线性减少。通过改善消化,将槲皮素添加到LCP中可以逆转BWG。
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引用次数: 1
Reassessing cow comfort measures on Canadian dairy farms after a recommendation of improvements 在提出改进建议后,重新评估加拿大奶牛场的奶牛舒适措施
IF 1 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-26 DOI: 10.1139/cjas-2022-0033
S. Palacio, A. Zambelis, S. Adam, R. Bergeron, D. Pellerin, D. Haley, T. DeVries, E. Vasseur
Abstract In a previous study (Assessment 1), an on-farm assessment tool was used to establish a standard across 60 Quebec dairy farms using animal-, stall-, and management-based measures of cow comfort. The objective of the present follow-up study (Assessment 2) was to perform a reassessment using an identical cow comfort assessment tool on a subset of the original farms expected to benefit most from applying the recommended changes, and to determine the effects of the modifications on cow comfort. From the subsample of farms, 24 reported applying stall modifications based on recommendations from Assessment 1 with the aim of promoting cow comfort (Adopters), while the remaining 10 farms did not (Non-adopters). The assessment tool included 19 target areas, grouped into 9 critical areas based on measures of cow comfort that considered housing, feed-water, health, and welfare. The on-farm report of Assessment 1 was found to increase producer awareness of issues in cow comfort measures on farms, as shown by an improvement in stall design according to recommendations based on cow body dimensions and reduced injury prevalence. Routine reassessment of cow comfort measures may be important to use in supporting target achievement improvements as part of welfare improvement strategies.
摘要在之前的一项研究(评估1)中,使用农场评估工具在魁北克60个奶牛场建立了一个标准,使用基于动物、摊位和管理的奶牛舒适度测量。本后续研究(评估2)的目的是使用相同的奶牛舒适度评估工具,对预计从应用建议的变更中受益最大的原始农场子集进行重新评估,并确定变更对奶牛舒适度的影响。从农场的子样本中,有24个农场报告称,根据评估1的建议,为了提高奶牛的舒适度,对摊位进行了修改(领养者),而其余10个农场则没有(非领养者)。该评估工具包括19个目标领域,根据奶牛舒适度的衡量标准,将其分为9个关键领域,其中考虑了住房、饲料水、健康和福利。评估1的农场报告提高了生产者对农场奶牛舒适措施问题的认识,根据基于奶牛身体尺寸的建议,摊位设计有所改进,受伤率有所降低。作为福利改善战略的一部分,对奶牛舒适度措施的常规重新评估对于支持目标实现的改善可能很重要。
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引用次数: 0
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Canadian Journal of Animal Science
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