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Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie最新文献

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Genetic complementation by somatic hybridization between pantothenate- and adenine-requiring auxotrophs of Datura innoxia P. Mill. 曼陀罗需要泛酸和腺嘌呤的营养缺陷体体细胞杂交的遗传互补。
P. Saxena, M. Mii, J. King
Auxotrophic complementation was achieved by protoplast fusion between two auxotrophic cell lines of Datura innoxia P. Mill. that required either pantothenate or adenine for their growth. Protoplast fusion was induced by polyethylene glycol enriched with calcium chloride at pH 6.8. Initial culture of fusion products in nonselective conditions increased the total number of somatic hybrids finally recovered under selective conditions. In addition to the prototrophy, the somatic hybrids regained the ability to turn green and some the ability to regenerate shoots on a cytokinin-supplemented medium. Both the parental cell lines grew as pale white callus without showing any sign of organogenesis, irrespective of the type and concentration of the cytokinin used. Approximately all the parental chromosomes (176 ± 8) were retained by the hybrid cell lines.Key words: protoplast, complementation, Datura.
研究了曼陀罗(Datura innoxia P. Mill)两种营养缺失细胞系间原生质体融合实现营养缺失互补。它们的生长要么需要泛酸盐要么需要腺嘌呤。在pH为6.8的条件下,富氯化钙聚乙二醇诱导原生质体融合。融合产物在非选择条件下的初始培养增加了在选择条件下最终恢复的体细胞杂交种总数。除了原生营养外,体细胞杂交体在补充细胞分裂素的培养基上恢复了变绿的能力,一些还恢复了再生芽的能力。无论细胞分裂素的类型和浓度如何,两种亲本细胞系都生长成淡白色的愈伤组织,没有显示出任何器官发生的迹象。杂交细胞系几乎保留了所有亲本染色体(176±8条)。关键词:原生质体;互补;曼陀罗;
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引用次数: 4
Chromosomal structure of homozygous common wheat lines obtained from (wheat × Agropyron) × Aegilops speltoides derivatives. II. A screening of paracentric inversions 小麦× Agropyron × Aegilops speltoides纯合子系的染色体结构。2。准中心反转的筛选
Luis Ortiz, Á. González, M. Chueca, Y. Cauderon
The presence of inversions is important in the evolution and cultivar differentiation of wheats, Even though we can't have conclusive cytological proof of the presence of inversions in wheat, we can use the indirect way of screening paracentric inversions through chromosome configuration at anaphase I and II. This work presents the comparison of 'Chinese Spring' structure (with respect to the presence of inverted segments) to (i) 52 homozygous wheat lines obtained in the progenies of the cross between a wheat × Agropyron intermedium substitution line and Aegilops speltoides homoeologous pairing promotor and (ii) three wheat cultivars ('Vilmorin 27', 'Ducat', and 'Tormes') that were used in developing the above mentioned lines. The three cultivars present inverted segments relative to 'Chinese Spring' structure. Some of these inverted segments are possibly maintained in some of the lines studied. Four of them show the primitive structure of 'Chinese Spring' even though none of the wheat parents had it. Fin...
倒位的存在在小麦的进化和品种分化中具有重要意义,尽管我们还没有确凿的细胞学证据证明倒位在小麦中存在,但我们可以利用间接的方法,通过染色体后期I和II的染色体结构来筛选顺中心倒位。本研究将“中国春”结构(关于反向片段的存在)与(i)在小麦与Agropyron中间代换系和Aegilops speltoides同源配对启动子杂交的后代中获得的52个纯合子小麦品系和(ii)用于开发上述品系的三个小麦品种('Vilmorin 27', 'Ducat'和'Tormes')进行了比较。三个品种呈现相对于“中国春”结构的倒节。在研究的某些直线中可能保留了一些这些倒置的线段。其中四个显示了“中国春天”的原始结构,即使没有小麦亲本。鱼翅……
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引用次数: 3
Random environmental variation and inbreeding: effects on pure-strain and hybrid populations of flour beetles (Tribolium) 随机环境变异和近交:对粉甲虫纯种和杂交种的影响
D. Wool, O. Bergerson
The effect of inbreeding and of random environmental variation on fitness characters was studied in small populations derived from 10 inbred laboratory strains of Tribolium castaneum (I) and in 18 ...
以10个实验室自交系和18个实验室自交系为材料,研究了近交系和随机环境变异对甜椒Tribolium castaneum (I)小群体适合度性状的影响。
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引用次数: 4
Genome analysis of Elymus alatavicus and E. batalinii (Poaceae: Triticeae) alatavicus和batalinii的基因组分析(禾本科:小麦科)
K. Jensen, D. R. Dewey, K. H. Asay
Elymus alatavicus (Drob.) A. Love and E. batalinii (Krasn.) A. Love were studied to determine (i) meiotic behaviour, (ii) the mode of reproduction, (iii) the relationship between the two species, (iv) genomic constitutions, and (v) the most logical taxonomic classification of both species. A series of F1 hybrids between E. alatavicus, E. batalinii, and six "analyzer" species were developed. Chromosome pairing was studied at metaphase I to identify genomic similarities or differences. The results showed that E. alatavicus and E. batalinii are caespitose, self-fertile allohexaploids (2n = 42) with the same genomic formula SSYYXX. The F1 hybrids between E. alatavicus and E. batalinii had complete pairing (21 bivalents) at metaphase I in 7% of the cells and almost complete pairing in the remaining cells. High chromosome pairing and partial fertility (4 seeds/plant) in the F1 hybrids shows that the two species are closely related. Hybrids were obtained between E. alatavicus or E. batalinii and the following "a...
羊腹草(草)A. Love和E. batalinii (Krasn)A.对爱进行了研究,以确定(i)减数分裂行为,(ii)繁殖模式,(iii)两个物种之间的关系,(iv)基因组构成,以及(v)两个物种最合乎逻辑的分类分类。培育了一系列阿拉塔乌、巴塔利乌和6个“分析”种的F1杂交品种。染色体配对在中期I进行研究,以确定基因组的相似性或差异性。结果表明,alatavicus和batalinii是具有相同基因组公式SSYYXX的单生、自育的异源六倍体(2n = 42)。alatavicus和batalinie的F1杂交在I中期有7%的细胞完全配对(21个二价),其余细胞几乎完全配对。杂种F1的高染色体配对和部分育性(4粒/株)表明两种亲缘关系密切。结果表明:alatavicus或batalinii与以下“a…
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引用次数: 8
Genetic modifications of dimorphic incompatibility in the Turnera ulmifolia L. complex (Turneraceae) 芜菁复合体二形不亲和性的遗传修饰
J. Shore, S. Barrett
Diploid and tetraploid populations of Turnera ulmifolia are distylous and exhibit a strong self-incompatibility system. Distyly is governed by a single locus with two alleles. Several self-compatible variants were, however, obtained and the nature and genetic control of self-compatibility was assessed using controlled crosses. The study documented the occurrence of self-compatible variants in four contrasting situations. These included the following. (i) Self-compatibility in a diploid short-styled variant. The gene(s) governing self-compatibility interact with the distyly locus and are expressed only in short-styled plants. When tetraploids carrying the genes were synthesized, self-incompatibility reappeared. (ii) Self-compatibility occurred in a cross between geographically separate diploid populations. Self-compatibility appeared sporadically in the F1. Crosses revealed that self-compatibility is likely under polygenic control. (iii) Low levels of self-compatibility occurred in a tetraploid population....
杜鹃的二倍体和四倍体群体是二花的,表现出很强的自交不亲和系统。disyly由一个带有两个等位基因的位点控制。然而,获得了几个自亲和变异,并利用控制杂交评估了自亲和的性质和遗传控制。该研究记录了自相容变异在四种不同情况下的发生。其中包括以下内容。(i)二倍体短花柱变异的自亲和性。控制自亲和性的基因只在短柱植物中表达。当携带基因的四倍体合成时,再次出现自交不亲和。(ii)自亲和发生在地理上分离的二倍体群体间的杂交中。在F1中偶有自亲和。杂交表明,自亲和性可能受多基因控制。(iii)低水平的自交亲和发生在四倍体群体....
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引用次数: 22
The inheritance of genetic variation in rye (Secale cereale) affecting homoeologous chromosome pairing in hybrids with bread wheat (Triticum aestivum) 黑麦遗传变异对面包小麦(Triticum aestivum)杂种同源染色体配对的影响
P. Gupta, G. Fedak
Chiasmata frequencies ranging from 0.07 to 10.40 per cell were recorded in 125 hybrid plants derived from wheat × F1 rye crosses. These included 89 plants belonging to 28 families from the Triticum aestivum 'Chinese Spring' × Secale cereale F1 ('Petkus' × 'Prolific') cross and 36 plants belonging to 11 families from the 'Chinese Spring' × F1 ('Prolific' × 'Puma') cross. The data were analyzed to study the inheritance of genetic variation in rye affecting homoeologous chromosome pairing. The results were particularly interesting in the former case where segregation for major genes was evident. First, in this cross, a bimodal distribution was observed and second, chiasmata frequencies ranging from 6.11 to 10.40 per cell were observed in three families but without any hybrid falling in either the range of 3.0 to 6.0 or in the range of 6.11 to 9.82, showing discontinuous distribution. It was concluded that the genetic system in 'Petkus' differs from that in 'Prolific', and that genes both with major effects a...
125个小麦× F1黑麦杂交植株的杂交频率为0.07 ~ 10.40个/细胞。其中,Triticum aestivum‘Chinese Spring’× Secale cereale F1(‘Petkus’ב高产’)杂交28科89株,‘Chinese Spring’× F1(‘高产’בPuma’)杂交11科36株。分析了黑麦遗传变异对同源染色体配对的影响。在前一种情况下,结果特别有趣,因为主基因的分离是明显的。在该杂交中,首先观察到双峰分布;其次,在3个科中观察到交叉频率在每细胞6.11 ~ 10.40次之间,但在3.0 ~ 6.0或6.11 ~ 9.82范围内没有杂交,呈不连续分布。结论是,“Petkus”的遗传系统与“多产”的遗传系统不同,并且具有主要影响的基因都是…
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引用次数: 16
Gene interactions in Saccharomyces cerevisiae resulting in high-level resistance to the herbicide sulfometuron methyl 酿酒酵母菌基因相互作用导致对除草剂甲基磺脲的高水平抗性
G. H. Rank
The multiple drug resistant mutant pdrl-1 was used to select for independent isolates with resistance to high levels of the herbicide sulfometuron methyl. Tetrad analyses of isolates identified a p...
利用多重耐药突变体pdrl-1筛选了对高水平除草剂甲基磺脲具有抗性的独立分离株。四分体分析鉴定出一株p…
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引用次数: 10
Chromosomal location of seed storage protein genes in the genome of Elytrigia elongata 长叶鞘翅种子贮藏蛋白基因的染色体定位
J. Dvorak, D. Kasarda, M. D. Dietler, E. J. Lew, O. Anderson, J. C. Litts, P. Shewry
Additions of complete and telocentric chromosomes of Elytrigia elongata (Host) Nevski in Triticum aestivum L. 'Chinese Spring' were employed to assign the genes coding for seed storage proteins to ...
小麦长Elytrigia elongata(寄主)neski完整染色体和远心染色体的添加。利用“中国春”将编码种子贮藏蛋白的基因分配给……
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引用次数: 26
Heterochromatin composition and nucleolus organizer activity in four canid species. 四种犬科动物的异染色质组成和核仁组织器活性。
B Mayr, G Geber, H Auer, M Kalat, W Schleger

Sequential staining with a counterstain-contrasted fluorescent banding technique (chromomycin A3-distamycin A-DAPI) revealed the occurrence of distamycin A-4,6-diamidino-2-phenylindole (DA-DAPI) staining heterochromatin in the centromeric regions of chromosomes 33, 36, 37, and 38 in the wolf (Canis lupus pallipes) and of chromosomes 13, 16, and 23 in the blue fox (Alopex lagopus). The red fox (Vulpes vulpes) lacked such regions. Staining with DAPI--actinomycin D produced a QFH-type banding pattern with clearcut differences in the staining behaviour of DA-DAPI positive regions between these three canid species. Staining with the fluorochrome D 287/170 did not preferentially highlight any of the DA-DAPI positive regions in any of them. Counterstain-enhanced chromomycin A3 R-banding and studies of nucleolus organizer region location and activity confirmed a close relationship between the karyotype of the wolf and the domestic dog. Few heterochromatic marker bands were encountered in these two species, but heterochromatin polymorphism was evident in the blue fox.

用反染色对比荧光带技术(染色霉素A3-distamycin a - dapi)连续染色发现,狼(Canis lupus pallipes)和蓝狐(Alopex lagopus)染色体13、16和23号染色体的第33、36、37和38号染色体的着丝粒区存在着distamycin a -4,6-二氨基-2-苯基吲哚(DA-DAPI)染色的异染色质。红狐(Vulpes Vulpes)没有这样的区域。DAPI-放线菌素D染色产生qfh型带状图案,在这三种犬科动物之间DA-DAPI阳性区域的染色行为明显不同。用荧光染料d287 /170染色没有优先突出任何DA-DAPI阳性区域。反染色增强的chromomycin A3 r -带和核仁组织区位置和活性的研究证实了狼和家犬的核型密切相关。在这两个物种中很少发现异染色质标记带,但在蓝狐中异染色质多态性很明显。
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引用次数: 9
Variability in the effect of alcohol on alcohol metabolizing enzymes may determine relative sensitivity to alcohols: a new hypothesis. 酒精对酒精代谢酶影响的变异性可能决定了对酒精的相对敏感性:一个新的假设。
S M Singh

Individual and racial differences in response to alcohol and with respect to alcoholism have strong genetic predispositions. Most studies on the actual genetic determinants have concentrated on the isozymes of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), the two enzymes of the primary pathway of alcohol metabolism. Although few "activity" variants (associated with mutations in the structural genes) of the two enzymes are known to exist in susceptible groups, these observations do not offer an adequate explanation for the observed variability in response to alcohols in the population. Some recent studies have reported alterations in the specific activity of the two enzymes following exposure to alcohol for different lengths of time in man, rat, and mice. The induction-repression so observed is hypothesized to be regulated by one or more inducibility genetic elements (IGE) associated with the structural loci of the two enzymes. Variability in IGE will permit a genotype (individual) specific response in ADH and ALDH specific activity when challenged with a given level of alcohol. Considering the relative toxicity of acetaldehyde, the primary metabolite of this pathway, the resistant individuals would be expected to show ALDH induction. Conversely, the susceptible individuals should respond to alcohol by ALDH repression. The ability of an individual to show induction or repression following alcohol ingestion will depend on his or her IGE genotype(s) associated with specific enzyme loci. Also, the degree of polymorphism at these loci would be expected to be extensive and yet population and race specific. Once experimentally established, this approach could have important implications in screening, counselling, prevention, and in novel approaches to treatment.

个体和种族对酒精和酒精中毒的反应差异有很强的遗传倾向。大多数关于实际遗传决定因素的研究都集中在酒精代谢主要途径的两种酶乙醇脱氢酶(ADH)和乙醛脱氢酶(ALDH)同工酶上。虽然已知在易感人群中存在这两种酶的少数“活性”变异(与结构基因突变相关),但这些观察结果并不能充分解释在人群中观察到的对酒精反应的变异性。最近的一些研究报道,在人、大鼠和小鼠中,这两种酶在暴露于酒精的不同时间长度后的特定活性发生了变化。观察到的诱导-抑制被假设是由一个或多个与两种酶的结构位点相关的诱导遗传元件(IGE)调节的。在给定水平的酒精刺激下,IGE的变异性将允许ADH和ALDH特异性活性的基因型(个体)特异性反应。考虑到该途径的主要代谢物乙醛的相对毒性,预计耐药个体会表现出ALDH诱导。相反,易感个体对酒精的反应应该是ALDH抑制。个体在饮酒后表现出诱导或抑制的能力取决于其与特定酶位点相关的IGE基因型。此外,这些位点的多态性程度预计是广泛的,但群体和种族特异性。一旦实验确定,这种方法可能在筛查、咨询、预防和新的治疗方法方面具有重要意义。
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引用次数: 10
期刊
Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie
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