Pınar Aksu Kılıçle, E. Koç, A. Doğan, Süleyman Gül, A. Doğan, Y. Ersan
: The aim of this study was to investigate the effects of acrylamide on Capoeta capoeta (Guldensttead 1773) by histopathological, electrophoretic and biochemical methods. Capoeta capoeta caught from Kars stream were used in the study. The fish were divided into 5 groups, each containing 10 pieces, and placed in 300 liter tanks with tap water. Group 1 was kept as a negative control. 20 mg / kg cyclophosphamide given to group 2 (i.p. positive control group), 10 mg/L acrylamide given to group 3, 20 mg/L acrylamide given to group 4 and 30 mg/L acrylamide given to group 5. After all groups were kept in tanks for 4 days, blood and tissue samples taken from fish were investigated by histopathological, electrophoretic and biochemical methods. As a result of the analyzes serum AST and ALT levels were decreased in the other groups compared to the negative control group and serum TAS levels were significantly increased in the 30 mg/L acrylamide group compared to the negative control group (P < 0.01). Compared with the negative control group, TOS levels were increased in all groups. When the electropherogram obtained from SDS-PAGE was examined, it was determined increases and decreases at 21 kD, 27 kD, 36 kD, 42 kD, 48 kD, 54 kD protein expressions in groups with different concentrations compared to the negative control group. It was observed that protein expressions were inhibited especially in the group treated with 20 mg/L acrylamide. As a result of histopathological examinations; increased degenerations were detected in the gill and liver tissues of fish due to the concentration of acrylamide. As a result; acrylamide treatment caused toxic effects on C. capoeta after this varying time intervals and concentrations.
{"title":"INVESTIGATION OF THE EFFECT OF ACRYLAMIDE ON CAPOETA CAPOETA (GULDENSTTEAD 1773) BY HISTOPATHOLOGICAL, ELECTROPHORETIC AND BIOCHEMICAL METHODS","authors":"Pınar Aksu Kılıçle, E. Koç, A. Doğan, Süleyman Gül, A. Doğan, Y. Ersan","doi":"10.48138/cjo.836674","DOIUrl":"https://doi.org/10.48138/cjo.836674","url":null,"abstract":": The aim of this study was to investigate the effects of acrylamide on Capoeta capoeta (Guldensttead 1773) by histopathological, electrophoretic and biochemical methods. Capoeta capoeta caught from Kars stream were used in the study. The fish were divided into 5 groups, each containing 10 pieces, and placed in 300 liter tanks with tap water. Group 1 was kept as a negative control. 20 mg / kg cyclophosphamide given to group 2 (i.p. positive control group), 10 mg/L acrylamide given to group 3, 20 mg/L acrylamide given to group 4 and 30 mg/L acrylamide given to group 5. After all groups were kept in tanks for 4 days, blood and tissue samples taken from fish were investigated by histopathological, electrophoretic and biochemical methods. As a result of the analyzes serum AST and ALT levels were decreased in the other groups compared to the negative control group and serum TAS levels were significantly increased in the 30 mg/L acrylamide group compared to the negative control group (P < 0.01). Compared with the negative control group, TOS levels were increased in all groups. When the electropherogram obtained from SDS-PAGE was examined, it was determined increases and decreases at 21 kD, 27 kD, 36 kD, 42 kD, 48 kD, 54 kD protein expressions in groups with different concentrations compared to the negative control group. It was observed that protein expressions were inhibited especially in the group treated with 20 mg/L acrylamide. As a result of histopathological examinations; increased degenerations were detected in the gill and liver tissues of fish due to the concentration of acrylamide. As a result; acrylamide treatment caused toxic effects on C. capoeta after this varying time intervals and concentrations.","PeriodicalId":9662,"journal":{"name":"Caucasian Journal of Science","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82766538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Harmankaya, A. Özcan, Ruya Kaya, Çağatay Özbey, O. Atakisi, K. Dalginli
: Pills including vitamins and minerals have been used as part of a sedentary lifestyle, malnutrition, aging, and age-related illnesses in developed countries. This study specifically focuses on the effect of ACE Plus Selenium given rabbits through intraperitoneal (i.p) injection on their total oxidant, antioxidant capacity (TOC, TAC), and nitric oxide (NO) levels. In this study, 0,5 ml/kg of normal saline was injected into rabbits in the control group and 0,5 ml/kg of ACE Plus Selenium was conducted to the treatment group twice (every other day) via i.p. route. Following the injection, plasmas of blood samples obtained in the second and fourth days were separated, and stored at –20 º C until the analysis. Plasma TAC, TOC and NO levels were determined spectrophotometrically. The TOC, TAC and NO levels of the rabbits were compared to the control group statistically. While TOC levels were observed to decrease (p<0,05) on the fourth day, the NO levels increased (p<0,01) on the second day and became normal on the fourth day. Further no statistical alteration was observed in the TAC levels. As a result, it can be concluded that ACE Plus Selenium had no effect on TAC level, it may decrease TOC levels in parallel to the decline in oxidative stress; further, it can increase NO levels acutely as a result of α-tocopherol and ascorbate ingredients.
{"title":"Effect of ACE Plus Selenium on Total Antioxidant/Oxidant Capacity and Nitric Oxide Levels in Rabbits","authors":"A. Harmankaya, A. Özcan, Ruya Kaya, Çağatay Özbey, O. Atakisi, K. Dalginli","doi":"10.48138/cjo.830176","DOIUrl":"https://doi.org/10.48138/cjo.830176","url":null,"abstract":": Pills including vitamins and minerals have been used as part of a sedentary lifestyle, malnutrition, aging, and age-related illnesses in developed countries. This study specifically focuses on the effect of ACE Plus Selenium given rabbits through intraperitoneal (i.p) injection on their total oxidant, antioxidant capacity (TOC, TAC), and nitric oxide (NO) levels. In this study, 0,5 ml/kg of normal saline was injected into rabbits in the control group and 0,5 ml/kg of ACE Plus Selenium was conducted to the treatment group twice (every other day) via i.p. route. Following the injection, plasmas of blood samples obtained in the second and fourth days were separated, and stored at –20 º C until the analysis. Plasma TAC, TOC and NO levels were determined spectrophotometrically. The TOC, TAC and NO levels of the rabbits were compared to the control group statistically. While TOC levels were observed to decrease (p<0,05) on the fourth day, the NO levels increased (p<0,01) on the second day and became normal on the fourth day. Further no statistical alteration was observed in the TAC levels. As a result, it can be concluded that ACE Plus Selenium had no effect on TAC level, it may decrease TOC levels in parallel to the decline in oxidative stress; further, it can increase NO levels acutely as a result of α-tocopherol and ascorbate ingredients.","PeriodicalId":9662,"journal":{"name":"Caucasian Journal of Science","volume":"549 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88076799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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