Pub Date : 2019-05-31DOI: 10.26650/EURJBIOL.2019.0003
Gokce Ustundag, K. Büyükgüzel, E. Büyükgüzel
ABSTRACT Objective: Laboratory research on management strategies such as biological and chemical control usually demands rearing high numbers of high-quality insects. For this reason, there is a need for detailed information about which antimicrobial agents should be added to their diets in order to obtain high quality insects. Materials and methods: Niclosamide, which is an anthelmintic derivative of salisilanilid, was added in amounts of 100, 200, 400 and 800 mg/L into artificial diets of Drosophila melanogaster (Meigen). The effects on survival rate, developmental time and adult longevity of the insects were examined. Furthermore, the effect of this anthelmintic antibiotic on important oxidative stress indicators; lipid peroxidation product, malondialdehyde (MDA) and protein oxidation products; protein carbonyl (PCO) contents and a detoxification enzyme, glutathione S-transferase (GST) activity in 3rd instar larvae, pupae and adult stage of the insect were also investigated. Results: Compared with those insects on the control diet, the tested concentrations of niclosamide significantly decreased survival rate in all developmental stages of the insect. The control diet produced 94.0 ± 1.0 % of 3rd stage larvae whereas in the highest concentration (800 mg/L) this ratio decreased to 14.00 % ± 1.73. While 42.08 ± 0.50 % of the adults survived in the control diet, longevity decreased to 2.30 ± 0.15 days in the highest concentration. All concentrations of niclosamide resulted in increased MDA contents in the last larval stage of D. melanogaster and concentrations of 100, 200 and 800 mg/L niclosamide increased pupal MDA content in comparison to the control group. At low concentrations of niclosamide, PCO decreased in the last stage of larvae. The diet with 400 mg/L niclosamide concentration significiantly increased GST activity in pupal stage. Conclusion: The results of this work indicated that the negative effects of niclosamide on biological characteristics of D. melanogaster are due to an increase in the oxidative stress and crippled detoxification capacity of the insect.
{"title":"The Effect of Niclosamide on Certain Biological and Biochemical Properties of Drosophila melanogaster","authors":"Gokce Ustundag, K. Büyükgüzel, E. Büyükgüzel","doi":"10.26650/EURJBIOL.2019.0003","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2019.0003","url":null,"abstract":"ABSTRACT Objective: Laboratory research on management strategies such as biological and chemical control usually demands rearing high numbers of high-quality insects. For this reason, there is a need for detailed information about which antimicrobial agents should be added to their diets in order to obtain high quality insects. Materials and methods: Niclosamide, which is an anthelmintic derivative of salisilanilid, was added in amounts of 100, 200, 400 and 800 mg/L into artificial diets of Drosophila melanogaster (Meigen). The effects on survival rate, developmental time and adult longevity of the insects were examined. Furthermore, the effect of this anthelmintic antibiotic on important oxidative stress indicators; lipid peroxidation product, malondialdehyde (MDA) and protein oxidation products; protein carbonyl (PCO) contents and a detoxification enzyme, glutathione S-transferase (GST) activity in 3rd instar larvae, pupae and adult stage of the insect were also investigated. Results: Compared with those insects on the control diet, the tested concentrations of niclosamide significantly decreased survival rate in all developmental stages of the insect. The control diet produced 94.0 ± 1.0 % of 3rd stage larvae whereas in the highest concentration (800 mg/L) this ratio decreased to 14.00 % ± 1.73. While 42.08 ± 0.50 % of the adults survived in the control diet, longevity decreased to 2.30 ± 0.15 days in the highest concentration. All concentrations of niclosamide resulted in increased MDA contents in the last larval stage of D. melanogaster and concentrations of 100, 200 and 800 mg/L niclosamide increased pupal MDA content in comparison to the control group. At low concentrations of niclosamide, PCO decreased in the last stage of larvae. The diet with 400 mg/L niclosamide concentration significiantly increased GST activity in pupal stage. Conclusion: The results of this work indicated that the negative effects of niclosamide on biological characteristics of D. melanogaster are due to an increase in the oxidative stress and crippled detoxification capacity of the insect.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80034465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-31DOI: 10.26650/EURJBIOL.2019.0001
L. Bat, F. Şahin, Ayşah Öztekin
ABSTRACT Objective: Metal bioaccumulation of the bivalve Mytilaster lineatus, based on different seasons and stations was investigated in Sinop Peninsula of the Black Sea. Soft tissues of mussels from Gazibey Rock and Icliman were taken during the spring and the summer of the year 2016 and were analyzed for Hg, Cd, Pb, Cu and Zn. Methods: The soft tissues of the mussels digested with Suprapur® HNO3 (nitric acid) using a Milestone Systems, Start D 260 microwave digestion system. The accuracy and precision of the method was verified by the simultaneous analysis of the certified reference materials NIST 2976 (mussel tissue) for the samples. The Inductively Coupled Plasma–Mass Spectrometer (ICP-MS), Agilent Technologies, 7700x was used for metal analyses. Results: Results showed that higher concentrations of almost all metals in Icliman than those in Gazibey Rock. However, no significant differences were detected between seasons except for both Cu and Zn. The metal amounts of M. lineatus ranged between 0.024-0.035, 0.054-0.072, 0.13-0.25, 0.64-0.85 and 6-20 mg kg-1 wet wt. for Hg, Cd, Pb, Cu and Zn, respectively. Conclusions: The measured metals in both sampling areas did not exceed the threshold set by the European Commission and Turkish Food Codex. The results of this study also confirmed the potential of M. lineatus to be used as bio-indicators of heavy metal pollution.
{"title":"Metal Bioaccumulation of Mytilaster lineatus (Gmelin, 1791) Collected From Sinop Coast in the Southern Black Sea","authors":"L. Bat, F. Şahin, Ayşah Öztekin","doi":"10.26650/EURJBIOL.2019.0001","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2019.0001","url":null,"abstract":"ABSTRACT Objective: Metal bioaccumulation of the bivalve Mytilaster lineatus, based on different seasons and stations was investigated in Sinop Peninsula of the Black Sea. Soft tissues of mussels from Gazibey Rock and Icliman were taken during the spring and the summer of the year 2016 and were analyzed for Hg, Cd, Pb, Cu and Zn. Methods: The soft tissues of the mussels digested with Suprapur® HNO3 (nitric acid) using a Milestone Systems, Start D 260 microwave digestion system. The accuracy and precision of the method was verified by the simultaneous analysis of the certified reference materials NIST 2976 (mussel tissue) for the samples. The Inductively Coupled Plasma–Mass Spectrometer (ICP-MS), Agilent Technologies, 7700x was used for metal analyses. Results: Results showed that higher concentrations of almost all metals in Icliman than those in Gazibey Rock. However, no significant differences were detected between seasons except for both Cu and Zn. The metal amounts of M. lineatus ranged between 0.024-0.035, 0.054-0.072, 0.13-0.25, 0.64-0.85 and 6-20 mg kg-1 wet wt. for Hg, Cd, Pb, Cu and Zn, respectively. Conclusions: The measured metals in both sampling areas did not exceed the threshold set by the European Commission and Turkish Food Codex. The results of this study also confirmed the potential of M. lineatus to be used as bio-indicators of heavy metal pollution.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86961189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-31DOI: 10.26650/EURJBIOL.2019.0006
Aylin Sancakli, Engin Kaptan
Objective: Abnormal glycosylation is a universal aspect of cancer cells. The altered glycosylation pattern has been originated from changings in expression of glycosylation enzymes which are up-regulating in reply to some oncoproteins in the biosynthetic pathway of glycans. In this study, it was aimed to show the presence of terminal α-2,3, α-2,6 sialic acid and α-1,6/α-1,2 fucose motifs in TPC-1 papillary thyroid cancer cells. Also it was aimed to examine the changes in viability and mobility of the cells after exogenously specific lectin treatment. Materials and Methods: In this study, the presence of terminal sugar residues in glycan chains on the cell surface was demonstrated using lectin histochemistry and lectin blotting techniques in TPC-1 cells. The changes in the cell viability and proliferation after lectin treatment were assessed using the WST-1 test. The Changes in the cell mobility after lectin treatment, however, were assessed using the wound healing test. Results: α-2,3, α-2,6 sialic acid and α-1,6/α-1,2 fucose motifs were widespread in the surface of TPC-1 cells. MAL-II (Maackia amurensis Lectin II) treatment increased the cell proliferation and mobility of TPC-1 cells. Although SNA (Sambucus nigra Aglutinin) and AAL (Aleuria aurantia Lectin) treatment did not significantly affect the cell proliferation, SNA and AAL treatment supported the mobility of TPC-1 cells. Conclusion: Lectin treatment affect cancerous properties differently depending on the cell type. Also lectin treatment can support the malignant behaviour of cancer. For this reason, it is necessary to understand the mechanisms of the lectin effect on the cancer cells.
{"title":"Lectin Treatment Affects Malignant Characteristics of TPC-1 Papillary Thyroid Cancer Cells","authors":"Aylin Sancakli, Engin Kaptan","doi":"10.26650/EURJBIOL.2019.0006","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2019.0006","url":null,"abstract":"Objective: Abnormal glycosylation is a universal aspect of cancer cells. The altered glycosylation pattern has been originated from changings in expression of glycosylation enzymes which are up-regulating in reply to some oncoproteins in the biosynthetic pathway of glycans. In this study, it was aimed to show the presence of terminal α-2,3, α-2,6 sialic acid and α-1,6/α-1,2 fucose motifs in TPC-1 papillary thyroid cancer cells. Also it was aimed to examine the changes in viability and mobility of the cells after exogenously specific lectin treatment. Materials and Methods: In this study, the presence of terminal sugar residues in glycan chains on the cell surface was demonstrated using lectin histochemistry and lectin blotting techniques in TPC-1 cells. The changes in the cell viability and proliferation after lectin treatment were assessed using the WST-1 test. The Changes in the cell mobility after lectin treatment, however, were assessed using the wound healing test. Results: α-2,3, α-2,6 sialic acid and α-1,6/α-1,2 fucose motifs were widespread in the surface of TPC-1 cells. MAL-II (Maackia amurensis Lectin II) treatment increased the cell proliferation and mobility of TPC-1 cells. Although SNA (Sambucus nigra Aglutinin) and AAL (Aleuria aurantia Lectin) treatment did not significantly affect the cell proliferation, SNA and AAL treatment supported the mobility of TPC-1 cells. Conclusion: Lectin treatment affect cancerous properties differently depending on the cell type. Also lectin treatment can support the malignant behaviour of cancer. For this reason, it is necessary to understand the mechanisms of the lectin effect on the cancer cells.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91156902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-31DOI: 10.26650/EURJBIOL.2019.0010
L. Fernández, E. Sanabria, L. Quiroga
Objective: This study reports the presence of a copepod, affecting adults of the torrent catfish in a tributary of the San Juan River in the southern part of South America. Materials and methods: The fish (48.1-54.1 mm standard length) were collected in Los Bretes, San Juan province, Argentina. They were anesthetized, fixed in formalin, preserved in ethanol and deposited in the Ichthyological Collection of the FACEN. Results: Three torrent catfish (Trichomycterus corduvensis) examined were infested with nine copepod parasites (Lernaea cyprinacea), which were attached to the gill, urogenital opening, and base pectoral fin. Conclusion: According to our results, this is the first record of parasitism of Lernaea on the torrent catfish (T. corduvensis) and a new locality from the Andean Mountains of Argentina.
{"title":"nfestation of the Torrent Catfish (Siluriformes, Trichomycteridae) by an Exotic Crustacean (Copepoda, Lernaeidae) in Andean Mountains of Argentina","authors":"L. Fernández, E. Sanabria, L. Quiroga","doi":"10.26650/EURJBIOL.2019.0010","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2019.0010","url":null,"abstract":"Objective: This study reports the presence of a copepod, affecting adults of the torrent catfish in a tributary of the San Juan River in the southern part of South America. Materials and methods: The fish (48.1-54.1 mm standard length) were collected in Los Bretes, San Juan province, Argentina. They were anesthetized, fixed in formalin, preserved in ethanol and deposited in the Ichthyological Collection of the FACEN. Results: Three torrent catfish (Trichomycterus corduvensis) examined were infested with nine copepod parasites (Lernaea cyprinacea), which were attached to the gill, urogenital opening, and base pectoral fin. Conclusion: According to our results, this is the first record of parasitism of Lernaea on the torrent catfish (T. corduvensis) and a new locality from the Andean Mountains of Argentina.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87967672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-31DOI: 10.26650/EURJBIOL.2019.0004
F. Touray, C. Ergül
Objective: Urbanization has been reported to affect the biodiversity of myxomycetes. There is no data in the Bursa city on the diversity of myxomycete in the urban area. Therefore, we aim to determine the presence of corticolous myxomycetes on monumental trees that are located in the most populated province of Bursa city (Osmangazi and Yildirim). Materials and Methods: In August and October 2018, 100 barks of monumental trees located in the Osmangazi and Yildirim) county of Bursa was collected. Moist chamber culture technique was used for the isolation and identification of the species. Results: 16 species within 9 genera were identified. The species identified are listed, and six new species Didymium bahiense (Gottsberger), Didymium difforme (Pers.) S. F. Gray), Macbrideola martinii (Alexop. & Beneke), Macbrideola oblonga (Pando, Lado), Physarum gyrosum (Rost.), Physarum notabile (Macbr.) were recorded for Bursa city. Conclusion: This is the first report of corticolous myxomycetes on monumental trees in Bursa city center. It also added data and 6 new records of myxomycetes on the myxomycete biodiversity of Bursa.
目的:研究了城市化对黏菌生物多样性的影响。没有关于布尔萨市市区黏菌多样性的数据。因此，我们的目标是确定在布尔萨市人口最多的省份(Osmangazi和Yildirim)的纪念树上是否存在皮粘菌。材料和方法:2018年8月和10月，在Bursa的Osmangazi和Yildirim县收集了100棵纪念树的树皮。采用湿室培养技术对该菌种进行分离鉴定。结果:共鉴定出9属16种。列出了已鉴定的物种，并发现了6个新种:bahiense (Gottsberger)、Didymium difforme (Pers.)。S. F. Gray)， Macbrideola martini (Alexop)。布尔萨市有绒泡菌(Rost.)、绒泡菌(Macbr.)、长绒泡菌(Macbr.)。结论:这是首次在布尔萨市中心的参天大树上发现皮质粘菌。增加了法氏法氏菌黏菌生物多样性的资料和6条粘菌新记录。
{"title":"Record of Myxomycetes on Monumental Trees in Bursa City Center – Turkey","authors":"F. Touray, C. Ergül","doi":"10.26650/EURJBIOL.2019.0004","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2019.0004","url":null,"abstract":"Objective: Urbanization has been reported to affect the biodiversity of myxomycetes. There is no data in the Bursa city on the diversity of myxomycete in the urban area. Therefore, we aim to determine the presence of corticolous myxomycetes on monumental trees that are located in the most populated province of Bursa city (Osmangazi and Yildirim). Materials and Methods: In August and October 2018, 100 barks of monumental trees located in the Osmangazi and Yildirim) county of Bursa was collected. Moist chamber culture technique was used for the isolation and identification of the species. Results: 16 species within 9 genera were identified. The species identified are listed, and six new species Didymium bahiense (Gottsberger), Didymium difforme (Pers.) S. F. Gray), Macbrideola martinii (Alexop. & Beneke), Macbrideola oblonga (Pando, Lado), Physarum gyrosum (Rost.), Physarum notabile (Macbr.) were recorded for Bursa city. Conclusion: This is the first report of corticolous myxomycetes on monumental trees in Bursa city center. It also added data and 6 new records of myxomycetes on the myxomycete biodiversity of Bursa.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76038950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-18DOI: 10.26650/EURJBIOL.2018.18008
S. Zora, Turkan Yigitbasi, G. Ülfer, N. Emekli
DOI: 10.26650/EurJBiol.2018.18008 Objective: Ghrelin is a hormone with peptide structure. It has fatty tissue and increases appetite. Obesity is a multifactorial chronic disease characterized by an increase in fat tissue. Fat tissue, like the endocrine organ, triggers oxidative stress and can lead to the development of obesity-related pathologies. The purpose of this study is to examine the relationship between the ghrelin in blood, and oxidative stress and lipid parameters. Materials and Methods: The study was conducted with 61 obese and 24 healthy individuals. Ghreline levels were measured using the ELISA method, while total antioxidant status (TAS) and oxidant status (TOS), triglyceride (TG), total cholesterol (TC), HDL-cholesterol (HDL-C) and LDL cholesterol (LDL-C) levels were measured using the photometric method. Results: A negative correlation was found between body mass index (BMI) and ghrelin levels in the obese group (p 0.05). TAS was observed to be lower in obese compared to control group, while The Oxidative Stress Index (OSI) was found to be significantly higher than the obese group (p 0.05). Conclusion: Increasing obesity level (BMI) and decreasing ghrelin level were found to be correlated. New studies are needed in order to discover the changes in ghrelin level connected to oxidative stress.
{"title":"Serum Concentration of Ghrelin, Oxidative Stress and Lipid Parameters in Obese Subjects","authors":"S. Zora, Turkan Yigitbasi, G. Ülfer, N. Emekli","doi":"10.26650/EURJBIOL.2018.18008","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2018.18008","url":null,"abstract":"DOI: 10.26650/EurJBiol.2018.18008 Objective: Ghrelin is a hormone with peptide structure. It has fatty tissue and increases appetite. Obesity is a multifactorial chronic disease characterized by an increase in fat tissue. Fat tissue, like the endocrine organ, triggers oxidative stress and can lead to the development of obesity-related pathologies. The purpose of this study is to examine the relationship between the ghrelin in blood, and oxidative stress and lipid parameters. Materials and Methods: The study was conducted with 61 obese and 24 healthy individuals. Ghreline levels were measured using the ELISA method, while total antioxidant status (TAS) and oxidant status (TOS), triglyceride (TG), total cholesterol (TC), HDL-cholesterol (HDL-C) and LDL cholesterol (LDL-C) levels were measured using the photometric method. Results: A negative correlation was found between body mass index (BMI) and ghrelin levels in the obese group (p 0.05). TAS was observed to be lower in obese compared to control group, while The Oxidative Stress Index (OSI) was found to be significantly higher than the obese group (p 0.05). Conclusion: Increasing obesity level (BMI) and decreasing ghrelin level were found to be correlated. New studies are needed in order to discover the changes in ghrelin level connected to oxidative stress.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90573308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-18DOI: 10.26650/EUROJBIOL.2018.346175
Özgür Çanak, T. Akaylı
DOI : 10.26650/EuroJBiol.2018.346175 Objective : Gilt-head seabream (Sparus aurata) is an important species for Turkish aquaculture and bacterial diseases are one of the limiting factors for the production of this species. The aim of this study is the identification of the bacterial disease agents in cultured gilt-head seabream in Turkey and the determination of their antibacterial susceptibilities. Materials and Methods : In this study, 27 diseased gilt-head seabream samples between 3-130 g, showing various clinical symptoms, were examined bacteriologically. Bacterial isolates recovered from moribund fish samples were identified by using standard bacteriological methods and API 20E rapid identification strips. Antibiotic susceptibility tests of 10 different substances against these isolates were determined by using Kirby-Bauer disc diffusion method. Results : Vibrio anguillarum, V. scophthalmi, V. logei, V. harveyi, Pseudomonas anguilliseptica, P. stutzeri, Staphylococcus sp. and Micrococcus luteus isolates were isolated from diseased fish samples as pure and mixed infection. It was detected that API 20E may give erroneous results for fish pathogenic bacteria since they are not included in the database of the kit. Generally, enrofloxacin, florphenicol and oxytetracycline were found to be the most effective antibiotics against bacterial isolates. Conclusion : This study is the first report for V. scophthalmi, V. logei, V. harveyi, P. stutzeri and M. luteus infections of gilt-head seabream in Turkey.
{"title":"Bacteria Recovered from Cultured Gilt-Head Seabream (Sparus aurata) and their Antimicrobial Susceptibilities","authors":"Özgür Çanak, T. Akaylı","doi":"10.26650/EUROJBIOL.2018.346175","DOIUrl":"https://doi.org/10.26650/EUROJBIOL.2018.346175","url":null,"abstract":"DOI : 10.26650/EuroJBiol.2018.346175 Objective : Gilt-head seabream (Sparus aurata) is an important species for Turkish aquaculture and bacterial diseases are one of the limiting factors for the production of this species. The aim of this study is the identification of the bacterial disease agents in cultured gilt-head seabream in Turkey and the determination of their antibacterial susceptibilities. Materials and Methods : In this study, 27 diseased gilt-head seabream samples between 3-130 g, showing various clinical symptoms, were examined bacteriologically. Bacterial isolates recovered from moribund fish samples were identified by using standard bacteriological methods and API 20E rapid identification strips. Antibiotic susceptibility tests of 10 different substances against these isolates were determined by using Kirby-Bauer disc diffusion method. Results : Vibrio anguillarum, V. scophthalmi, V. logei, V. harveyi, Pseudomonas anguilliseptica, P. stutzeri, Staphylococcus sp. and Micrococcus luteus isolates were isolated from diseased fish samples as pure and mixed infection. It was detected that API 20E may give erroneous results for fish pathogenic bacteria since they are not included in the database of the kit. Generally, enrofloxacin, florphenicol and oxytetracycline were found to be the most effective antibiotics against bacterial isolates. Conclusion : This study is the first report for V. scophthalmi, V. logei, V. harveyi, P. stutzeri and M. luteus infections of gilt-head seabream in Turkey.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76274380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-18DOI: 10.26650/eurjbiol.2018.0013
M. Koçyi̇ği̇t, S. Kayıran
DOI: 10.26650/EurJBiol.2018.0013 Objective: The Baskonus Mountain (Kahramanmaras) is located in the transition zone of Irano-Turan and Mediterranean plant geographical regions and in the southern part of the Anatolian Diagonal. A specific region in the East Mediterranean basin consists of an area that holds great biological importance and is a world hotspot. In this study, the small protected areas of the Baskonus Mountain were determined. Materials and Methods: A total of 17 endemic and 9 rare taxa were selected based on their IUCN statutes, altitudes, and habitats. Their GPS coordinates were loaded on a computer and digitized in a map projection system. Distributions of the selected taxa were marked on a topographic map in the scale of 1/25.000. The 26 maps were overlapped so that the areas of plant biodiversity could be observed on the map. Results: Six small protected areas were defined on the Baskonus Mountain. Several threats and conservation suggestions were presented in this study. Conclusion: Local data about biodiversity sources are limited. Thus, conservation biologists and government offices should play active roles in formal educational settings and employ alternative methods to the community in Kahramanmaras.
{"title":"“Small Protected Areas” for Conservation Priorities in South Anatolia (Başkonuş Mountain-Kahramanmaraş)","authors":"M. Koçyi̇ği̇t, S. Kayıran","doi":"10.26650/eurjbiol.2018.0013","DOIUrl":"https://doi.org/10.26650/eurjbiol.2018.0013","url":null,"abstract":"DOI: 10.26650/EurJBiol.2018.0013 Objective: The Baskonus Mountain (Kahramanmaras) is located in the transition zone of Irano-Turan and Mediterranean plant geographical regions and in the southern part of the Anatolian Diagonal. A specific region in the East Mediterranean basin consists of an area that holds great biological importance and is a world hotspot. In this study, the small protected areas of the Baskonus Mountain were determined. Materials and Methods: A total of 17 endemic and 9 rare taxa were selected based on their IUCN statutes, altitudes, and habitats. Their GPS coordinates were loaded on a computer and digitized in a map projection system. Distributions of the selected taxa were marked on a topographic map in the scale of 1/25.000. The 26 maps were overlapped so that the areas of plant biodiversity could be observed on the map. Results: Six small protected areas were defined on the Baskonus Mountain. Several threats and conservation suggestions were presented in this study. Conclusion: Local data about biodiversity sources are limited. Thus, conservation biologists and government offices should play active roles in formal educational settings and employ alternative methods to the community in Kahramanmaras.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81950967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-18DOI: 10.26650/EUROJBIOL.2018.404499
T. Akaylı, Buket Aydın, Ç. Ürkü, Ozgecan Kayalar
DOI : 10.26650/EuroJBiol.2018.404499 Objective : In this study, the diagnosis of infection caused by Vibrio anguillarum in cultured rainbow trout (Oncorhynchus mykiss), in Turkey using bacteriological, histopathological and immunohistochemical methods was attempted. Materials and Methods : 15 rainbow trout samples showing signs of disease were investigated using bacteriological and histopatological methods. Results : Because of physiological and biochemical characteristics of bacteria growing on the medium, these bacteria were isolated and identified as V. anguillarum. As a result of the histopathologic examination of the tissues of diseased rainbow trout, hyperemia in the liver, degeneration of the epithelium of kidney tubules and necrosis in the interstitial area, enlargement in the gill filaments, cells having picnotic nucleus and several necrotic areas in the spleen were observed. It was also detected in the streptavidin-biotin staining method that the causative agent accumulates in the liver, spleen, kidney and also in the gills especially in the areas near around the blood vessels depending on the development stage of the disease. Conclusion : As a result, an immunoenzimatic method, streptavidin-biotin staining method was used in this study for the detection of the presence of V. anguillarum in the infected rainbow trout tissues.
{"title":"Diagnosis of Vibrio anguillarum in Cultured Rainbow Trout (Oncorhynchus mykiss) by Different Methods","authors":"T. Akaylı, Buket Aydın, Ç. Ürkü, Ozgecan Kayalar","doi":"10.26650/EUROJBIOL.2018.404499","DOIUrl":"https://doi.org/10.26650/EUROJBIOL.2018.404499","url":null,"abstract":"DOI : 10.26650/EuroJBiol.2018.404499 Objective : In this study, the diagnosis of infection caused by Vibrio anguillarum in cultured rainbow trout (Oncorhynchus mykiss), in Turkey using bacteriological, histopathological and immunohistochemical methods was attempted. Materials and Methods : 15 rainbow trout samples showing signs of disease were investigated using bacteriological and histopatological methods. Results : Because of physiological and biochemical characteristics of bacteria growing on the medium, these bacteria were isolated and identified as V. anguillarum. As a result of the histopathologic examination of the tissues of diseased rainbow trout, hyperemia in the liver, degeneration of the epithelium of kidney tubules and necrosis in the interstitial area, enlargement in the gill filaments, cells having picnotic nucleus and several necrotic areas in the spleen were observed. It was also detected in the streptavidin-biotin staining method that the causative agent accumulates in the liver, spleen, kidney and also in the gills especially in the areas near around the blood vessels depending on the development stage of the disease. Conclusion : As a result, an immunoenzimatic method, streptavidin-biotin staining method was used in this study for the detection of the presence of V. anguillarum in the infected rainbow trout tissues.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85782053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-18DOI: 10.26650/EURJBIOL.2018.18007
C. Sezer
DOI: 10.26650/EurJBiol.2018.18007 Objective: The anti-cancer activities of vanadium and its compounds have been widely investigated in cancer research recently. This is mainly attributed to vanadium and its compounds’ near ideal properties for being an anti-cancer agent. Most of the current classical chemotherapeutics used in cancer therapy are known to bring numerous strong side effects. Thus, there is a need to discover new drugs which have mild or no side effects and which are effective in low doses. For the treatment of breast cancer (a disease with a difficult and costly treatment process, high mortality, and which is especially prevalent in woman), novel drugs and approaches are required. With this in mind, this study investigates the potential therapeutic efficacies of vanadyl sulphate, a member of the vanadium compounds with ideal anti-cancer properties such as cytotoxicity, antiproliferative and proapoptotic activities on human breast adenocarcinoma cells (MCF-7) including morphological and ultrastructural changes. Materials and Methods: A MTT colorimetric assay was used for cell viability assessment. Morphological and ultrastructural changes were evaluated using confocal and transmission electron microscopy methods, respectively. The apoptosis stimulating property of vanadyl sulphate was tested under a flow cytometry. And also, cell cycle and proliferation inhibitory effects were examined using the immunohistochemistry technique.
{"title":"Multi-Mode Assessment Approach on Anti-Cancer Potency of Vanadium on Breast Cancer Cells","authors":"C. Sezer","doi":"10.26650/EURJBIOL.2018.18007","DOIUrl":"https://doi.org/10.26650/EURJBIOL.2018.18007","url":null,"abstract":"DOI: 10.26650/EurJBiol.2018.18007 Objective: The anti-cancer activities of vanadium and its compounds have been widely investigated in cancer research recently. This is mainly attributed to vanadium and its compounds’ near ideal properties for being an anti-cancer agent. Most of the current classical chemotherapeutics used in cancer therapy are known to bring numerous strong side effects. Thus, there is a need to discover new drugs which have mild or no side effects and which are effective in low doses. For the treatment of breast cancer (a disease with a difficult and costly treatment process, high mortality, and which is especially prevalent in woman), novel drugs and approaches are required. With this in mind, this study investigates the potential therapeutic efficacies of vanadyl sulphate, a member of the vanadium compounds with ideal anti-cancer properties such as cytotoxicity, antiproliferative and proapoptotic activities on human breast adenocarcinoma cells (MCF-7) including morphological and ultrastructural changes. Materials and Methods: A MTT colorimetric assay was used for cell viability assessment. Morphological and ultrastructural changes were evaluated using confocal and transmission electron microscopy methods, respectively. The apoptosis stimulating property of vanadyl sulphate was tested under a flow cytometry. And also, cell cycle and proliferation inhibitory effects were examined using the immunohistochemistry technique.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84347496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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