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Association of CASP8 rs3834129 and CTGF rs6918698 genotypes with susceptibility to colorectal cancer in a Mexican population. CASP8 rs3834129和CTGF rs6918698基因型与墨西哥人群结直肠癌易感性的关联
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-27 DOI: 10.14715/cmb/2024.70.11.2
Anilú Margarita Saucedo-Sariñana, Yuri Giovanna Vanessa Trujillo-Fernández, Clara Ibet Juarez-Vázquez, Miriam Yadira Godínez-Rodríguez, César de Jesús Tovar-Jácome, Patricio Barros-NúñeZ, Tomás Daniel Pineda-Razo, María Eugenia Marín-Contreras, Mónica Alejandra Rosales-Reynoso

Connective tissue growth factor (CTGF) and Caspase 8 (CASP8) have been implicated in cancer development and progression. Variants such as CASP8 rs3834129 (-652 6N I/D) and CTGF rs6918698 (-945 C>G) have been associated with several cancers, although their association is still debated between populations. This study investigates the possible association between the CASP8 rs3834129 and CTGF rs6918698 variants with  colorectal cancer (CRC) in Mexican patients. Genomic DNA was extracted from 250 CRC patients and 250 control subjects. The identification of CASP8 and CTGF variants was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. The association was determined by the odds ratio (OR) analysis and P values were adjusted by the Bonferroni correction. Patients carrying the D/D genotype for the CASP8 rs3834129 variant exhibited an increased susceptibility to CRC (P = 0.012). The D/D genotype was associated with older 50-year-old patients (P = 0.006). In addition, this same D/D genotype was associated with TNM II stage (P = 0.013) and rectal localization (P = 0.023). Additionally, patients carrying the G/G genotype for the CTGF rs6918698 variant showed a decreased susceptibility to CRC (P = 0.009), and in the sex stratification, this gene has protective role in males (P = 0.008). This same genotype was associated with decreased susceptibility to early TNM stages (I+II) (P = 0.023) and right-sided colon tumor localization (P = 0.002). There was no association between response to treatment and the variants analyzed. Our findings suggest that the CASP8 rs3834129 and CTGF rs6918698 variants have a significant impact on the development of CRC.

结缔组织生长因子(CTGF)和CASP8 (CASP8)与癌症的发生和发展有关。CASP8 rs3834129 (-652 6N I/D)和CTGF rs6918698 (-945 C>G)等变异与几种癌症有关,尽管它们在人群之间的相关性仍存在争议。本研究探讨了CASP8 rs3834129和CTGF rs6918698变异与墨西哥患者结直肠癌(CRC)之间的可能关联。从250例结直肠癌患者和250例对照组中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法鉴定CASP8和CTGF变异。通过优势比(OR)分析确定相关性,并通过Bonferroni校正调整P值。携带CASP8 rs3834129变体D/D基因型的患者对CRC的易感性增加(P = 0.012)。D/D基因型与50岁高龄患者相关(P = 0.006)。此外,相同的D/D基因型与TNM II分期(P = 0.013)和直肠定位(P = 0.023)相关。此外,携带CTGF rs6918698变异G/G基因型的患者对CRC的易感性降低(P = 0.009),在性别分层中,该基因在男性中具有保护作用(P = 0.008)。该基因型与早期TNM阶段(I+II)的易感性降低(P = 0.023)和右侧结肠肿瘤定位(P = 0.002)相关。对治疗的反应与所分析的变异之间没有关联。我们的研究结果表明,CASP8 rs3834129和CTGF rs6918698变体对CRC的发展有显著影响。
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引用次数: 0
Disease complex associated with begomoviruses infecting squash and cucumber in Saudi Arabia. 在沙特阿拉伯,与感染南瓜和黄瓜的begomovirus相关的复杂疾病。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-27 DOI: 10.14715/cmb/2024.70.11.8
Mahmoud Ahmed Amer, Zaheer Khalid, Khadim Hussain, Ibrahim Al-Shahwan, Mohammed Ali Al-Saleh

During the field visits in growing season of 2022 in Dammam Region of Saudi Arabia, begomovirus-like symptoms including leaf curling, leaf cupping, leaf distortion, vein thickening and reduced leaf size were observed in squash and cucumber fields. Twenty-five samples were collected from each crop and PCR amplification was done using general diagnostic begomovirus primers (AC-1048/AV-494 and Begomo I/Begomo II). The obtained results showed desired sized amplified DNA fragments (550 bp and 1.1 kb) according to the primer sites. Sequencing results were analyzed using BLAST and revealed the presence of three different bipartite begomoviruses which include Squash leaf curl virus (SqLCV) isolated from squash and cucmber, Watermelon chlorotic stunt virus (WmCSV) and Tomato leaf curl Palampur virus (ToLCPalV) isolated from squash. The highest nucleotide identity found was 99.4% with Egyptian SqLCV isolated from squash and the lowest similarity was 93.3% found with a USA isolate isolated from wheel cactus. Sequencing results of two isolates of WmCSV showed 100% sequence identity with each other, eight isolates from Palestine isolated from watermelon, two isolates from Mexico isolated from prickly pear cactus and Watermelon, one isolate from each Lebanon and Jordan isolated from melon and wild mustard respectively. The lowest identity (87%) was found with a Saudi Arabian isolate isolated from papaya. For ToLCPalV isolate showed the highest identity (100 %) with an already reported isolate of same virus from melon in Saudi Arabia and two isolates isolated from cucumber and cantaloupe in Iran. However, the lowest identity (95.3%) was found with an Indian isolate isolated from eggplant. This is the first investigation of complex viral disease caused by SqLCV, WmCSV and ToLCPalV on the basis of molecular characterization from squash and a SqLCV isolate from Cucumber in Saudi Arabia.

在沙特阿拉伯达曼地区2022年生长季节的实地考察中,在南瓜和黄瓜田观察到begomovira样症状,包括叶片卷曲、叶片拔罐、叶片扭曲、静脉增厚和叶片缩小。每株作物采集25份样品,采用通用诊断型贝古莫病毒引物(AC-1048/AV-494和Begomo I/Begomo II)进行PCR扩增,结果显示根据引物位点扩增的DNA片段大小分别为550 bp和1.1 kb。利用BLAST对测序结果进行分析,发现存在3种不同的双部begomov,分别为来自南瓜和黄瓜的南瓜卷叶病毒(SqLCV)、西瓜萎蔫病毒(WmCSV)和来自南瓜的番茄卷叶病毒(ToLCPalV)。与从南瓜中分离的埃及SqLCV核苷酸同源性最高为99.4%,与从美国轮状仙人掌分离的SqLCV核苷酸同源性最低为93.3%。2个WmCSV分离株序列同源性为100%,8个分离株来自巴勒斯坦西瓜,2个分离株来自墨西哥仙人掌和西瓜,黎巴嫩和约旦分别从甜瓜和野芥菜中分离1个。与从木瓜中分离的沙特阿拉伯分离株的同源性最低(87%)。ToLCPalV分离株与已报道的沙特阿拉伯甜瓜分离株和伊朗黄瓜和哈密瓜分离株的同源性最高(100%)。然而,从茄子中分离出的印度分离物的鉴定度最低(95.3%)。这是基于南瓜和沙特阿拉伯黄瓜的SqLCV分离物的分子特征,首次研究由SqLCV、WmCSV和ToLCPalV引起的复杂病毒病。
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引用次数: 0
Influence of Azadirachta indica leaf extracts on tumor necrosis factor-α and interleukin-6 in albino rats and its computational analysis. 印楝叶提取物对白化大鼠肿瘤坏死因子-α和白细胞介素-6的影响及其计算分析。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-27 DOI: 10.14715/cmb/2024.70.11.14
Aniza Khadam, Sobia Alyas, Naureen Zahra, Sohail Ahmad, Abid Sarwar, Ayaz Ali Khan, Tariq Aziz, Metab Alharbi, Abdullah F Alasmari

The current study was designed to investigate the effect of A. indica (Neem) leaf extracts (ethanolic and aqueous) in yeast-induced pyrexia and acetic acid-induced writhing in rat models to evaluate the antipyretic and analgesic biomarkers and its phytochemical screening with computational analysis. For the antipyretic activity model 60 albino rats (160-200g) of either sex were divided into 4 groups and all groups were injected with yeast to induce pyrexia. Out of 4 groups, first group (control) consisted of 6 rats, treated with normal saline, the second group (standard) comprised 6 rats, treated with paracetamol. Third and fourth experimental groups consisted of 48 rats, treated with A. indica leaf ethanolic and aqueous extracts at doses of (50, 100, 200 and 400mg/kg b.w). For analgesic activity group division was the same and all groups were injected with acetic acid to induce pain TNF-α and IL-6 levels were measured using ELISA kits after blood samples were taken and serums were separated. An acute toxicity study was performed. In molecular docking, nimbandiol and nimbolide were used as ligand molecules to target protein Tnf-α and IL-6. In both activities at the dose of 400mg/kg, group III showed significant inhibition (p<0.05). Biomarkers showed significant results at the dose of 400mg/kg. Phytochemical screening was performed to reveal the existence of various active constituents. In molecular docking, nimbandiol and nimbolide showed -5 and -5.3 binding energies respectively, as compared to the standard drug paracetamol with -4.2 binding energy to TNF-Alpha protein. Therefore, A. indica extracts can be used as a valuable drug for the treatment of pain and fever.

本研究旨在研究印楝叶提取物(乙醇提取物和水提取物)对酵母致热和醋酸致扭体大鼠模型的影响,以评估其解热镇痛生物标志物及其植物化学筛选。解热模型取60只白化大鼠(160 ~ 200g),雌雄同体,分为4组,每组均注射酵母解热。4组中,第一组(对照组)6只大鼠给予生理盐水治疗,第二组(标准组)6只大鼠给予扑热息痛治疗。第三和第四实验组48只大鼠,分别以50、100、200和400mg/kg b.w的剂量给药。镇痛活性分组相同,各组均注射醋酸致痛,取血分离血清后,采用ELISA试剂盒检测TNF-α、IL-6水平。进行了急性毒性研究。在分子对接中,nimbanddiol和nimbolide作为靶向蛋白Tnf-α和IL-6的配体分子。在400mg/kg剂量下,III组对两种活性均有显著抑制作用(p
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引用次数: 0
hMAGEA2 as a potential diagnostic and therapeutic target for melanoma progression and metastasis. hMAGEA2 作为黑色素瘤进展和转移的潜在诊断和治疗靶点。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.14
Junkoo Yi, Chae Yeon Kim, Hyeonjin Kim, Eun Gyung Kim, Do Yoon Kim, Yonghun Sung, Kang Dong Liu, Seong-Kyoon Choi, Zae Young Ryoo, Song Park, Myoung Ok Kim

The incidence of melanoma, a highly aggressive skin cancer, continues to increase worldwide, particularly among populations with lighter skin tones. The diagnostic challenge of melanoma lies in the absence of a distinctive clinical presentation, as its characteristics vary based on anatomical location, growth type, and histopathology. The melanoma-associated antigen (MAGE) gene family is differentially expressed in various human cancers, including melanoma. In this study, we explored the association between human MAGEA2 (hMAGEA2) expression and melanoma. Using a human melanoma tissue array, we confirmed that hMAGEA2 expression was higher in melanoma and metastatic melanoma than in normal tissues. Additionally, we used SK-MEL-5 and SK-MEL-28 cell lines to investigate the cellular and molecular mechanisms underlying melanoma progression and invasiveness. In SK-MEL-5 and SK-MEL-28 cells, hMAGEA2 overexpression accelerated cell proliferation. Conversely, the knockdown of hMAEGA2 reduced cell proliferation, colony formation, and migration significantly and induced arrest at the G2/M phase of the cell cycle. With respect to the molecular mechanism, the knockdown of hMAGEA2 decreased the phosphorylation of Akt, JNK, and p38 MAPK. Additionally, hMAGEA2 knockdown reduced tumor formation significantly at the in vivo level. Collectively, the robust correlation between hMAGEA2 and melanoma metastasis supports the potential utility of hMAGEA2 as both a diagnostic marker and novel therapeutic target for patients with melanoma metastasis.

黑色素瘤是一种侵袭性很强的皮肤癌,其发病率在全球范围内持续上升,尤其是在肤色较浅的人群中。黑色素瘤的诊断难题在于没有独特的临床表现,因为其特征因解剖位置、生长类型和组织病理学而异。黑色素瘤相关抗原(MAGE)基因家族在包括黑色素瘤在内的各种人类癌症中都有不同程度的表达。在这项研究中,我们探讨了人类 MAGEA2(hMAGEA2)表达与黑色素瘤之间的关联。利用人体黑色素瘤组织阵列,我们证实黑色素瘤和转移性黑色素瘤中 hMAGEA2 的表达高于正常组织。此外,我们还利用 SK-MEL-5 和 SK-MEL-28 细胞系研究了黑色素瘤进展和侵袭性的细胞和分子机制。在SK-MEL-5和SK-MEL-28细胞中,hMAGEA2过表达会加速细胞增殖。相反,敲除hMAEGA2会显著减少细胞增殖、集落形成和迁移,并诱导细胞周期停滞在G2/M期。在分子机制方面,敲除 hMAGEA2 会降低 Akt、JNK 和 p38 MAPK 的磷酸化。此外,在体内水平上,hMAGEA2的敲除还能显著减少肿瘤的形成。总之,hMAGEA2与黑色素瘤转移之间的密切关联支持了hMAGEA2作为黑色素瘤转移患者的诊断标志物和新型治疗靶点的潜在用途。
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引用次数: 0
C-terminal tagging enhances the detection sensitivity of interlekin receptor type 1. C 端标记提高了 1 型交联蛋白受体的检测灵敏度。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.7
Ayuko Moriyama, Saya Imaoka, Tsuyoshi Sasagawa, Machi Hosaka, Isao Kato, Hiroki Tamura, Rie Takeuchi, Mariko Tsunoda, Masatake Asano

Substances released outside of the cells during cell necrosis are collectively called danger-associated molecular patterns (DAMPS) or alarmins. A pro-inflammatory cytokine, interleukin-1α (IL-1α) is known as a typical alarmin. IL-1α transmits signals by binding to IL-1 receptor 1 (IL-1R1), type I protein, expressed on the cell membrane of target cells, but detection of IL-1R1 at the protein and mRNA levels is difficult. Although the reasons are not elucidated, we attempted to add the HiBiT-tag to the N-terminus (N'-R1) or C-terminus (C'-R1) of IL-1R1 to examine whether the detection sensitivity can be augmented. Increase in detection sensitivity will allow the investigation of its function and subcellular localization much further. Using uterine cervical cancer-derived HeLa cells and its derivative CR-R1-4 cells lacking IL-1R1, C'-R1 was demonstrated to significantly increase the detection sensitivity of IL-1R1. Furthermore, the signal transduction function of neither N'-R1 nor C'-R1 was affected. Immunofluorescence cell staining revealed that wild-type IL-1R1 is mainly localized in the nucleus, whereas C'-R1 is localized both in the nucleus and the cytoplasm. The above results showed that adding a tag to the C-terminus of IL-1R1 increases detection sensitivity while maintaining its function. In the future, we would like to further investigate the relationship between changes in the intracellular localization of C'-R1 and increases in detection sensitivity.

细胞坏死时释放到细胞外的物质统称为危险相关分子模式(DAMPS)或警报素。白细胞介素-1α(IL-1α)是一种典型的促炎细胞因子。IL-1α 通过与表达在靶细胞细胞膜上的 I 型蛋白 IL-1 受体 1(IL-1R1)结合来传递信号,但很难在蛋白和 mRNA 水平上检测到 IL-1R1。虽然原因尚不清楚,但我们尝试在 IL-1R1 的 N 端(N'-R1)或 C 端(C'-R1)添加 HiBiT 标记,以研究是否能提高检测灵敏度。检测灵敏度的提高将有助于进一步研究 IL-1R1 的功能和亚细胞定位。利用子宫颈癌衍生的 HeLa 细胞及其缺乏 IL-1R1 的衍生物 CR-R1-4 细胞,证明 C'-R1 能显著提高 IL-1R1 的检测灵敏度。此外,N'-R1 和 C'-R1 的信号转导功能均未受到影响。免疫荧光细胞染色显示,野生型 IL-1R1 主要定位于细胞核,而 C'-R1 则同时定位于细胞核和细胞质。上述结果表明,在 IL-1R1 的 C 端添加标签能在保持其功能的同时提高检测灵敏度。今后,我们希望进一步研究 C'-R1 细胞内定位的变化与检测灵敏度提高之间的关系。
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引用次数: 0
Effect of periodontal therapy on serum and salivary Interleukin-1 beta (IL-1β) and malondialdehyde levels in chronic periodontitis. 牙周治疗对慢性牙周炎患者血清和唾液中白细胞介素-1β(IL-1β)及丙二醛水平的影响。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.22
Haween T Nanakaly, Sardar Nouri Ahmed, Hozan Warya Azeez

Chronic periodontitis (CP) is distinguished by an inflammatory reaction and the presence of oxidative stress (OS), which has consequences for overall health. Interleukin-1β (IL-1β) and malondialdehyde (MDA) serve as markers of inflammation and OS, respectively. Analyzing the alterations in their reaction to periodontal therapy can provide valuable insights into the management and monitoring of CP progression. The current study aimed to evaluate the impact of non-surgical periodontal therapy (NST) on IL-1β and MDA levels in the serum and saliva of CP patients and explore their correlation with clinical periodontal indices post-therapy. There were 60 participants in this research, aged 33 to 50, equally split between thirty periodontally healthy controls and 30 patients with CP. Measures were taken of the clinical periodontal parameters, including the bleeding index, probing pocket depth, gingival index, and plaque index. Saliva and blood samples were collected for IL-1β and MDA analysis using ELISA and spectrophotometrically. CP patients received scaling and root planning (SRP) as part of phase I periodontal therapy, and after six weeks, we reevaluated clinical parameters and IL-1β and MDA levels. In CP patients, both saliva and serum IL-1β and MDA levels significantly increased alongside worsening clinical periodontal parameters compared with periodontally healthy individuals. phase I periodontal therapy led to a notable decrease in both saliva and serum IL-1β and MDA levels, accompanied by improvements in clinical parameters. Additionally, following six weeks of scale and root planning treatment, our data showed a strong positive relationship between salivary IL-1β and MDA levels with PPD and CAL. SRP therapy is effective in managing periodontal health, as evidenced by a significant decrease in clinical parameters and biomarker levels after treatment for CP patients. This suggests that salivary IL-1β and MDA may be useful biomarkers for indicating the severity of periodontal disease and the effectiveness of treatment.

慢性牙周炎(CP)的特征是炎症反应和氧化应激(OS)的存在,而氧化应激会影响整体健康。白细胞介素-1β(IL-1β)和丙二醛(MDA)分别是炎症和氧化应激的标志物。分析它们对牙周治疗的反应变化可为管理和监测 CP 的进展提供有价值的见解。本研究旨在评估非手术牙周治疗(NST)对 CP 患者血清和唾液中 IL-1β 和 MDA 水平的影响,并探讨它们与治疗后临床牙周指标的相关性。这项研究共有 60 名参与者,年龄在 33 岁至 50 岁之间,其中 30 人为牙周健康的对照组,30 人为 CP 患者。研究人员测量了临床牙周参数,包括出血指数、探诊袋深度、牙龈指数和牙菌斑指数。采集唾液和血液样本,使用 ELISA 和分光光度法分析 IL-1β 和 MDA。作为牙周治疗第一阶段的一部分,CP 患者接受了洗牙和牙根规划(SRP)治疗,六周后,我们重新评估了临床参数、IL-1β 和 MDA 水平。与牙周健康的人相比,CP 患者唾液和血清中的 IL-1β 和 MDA 水平都明显升高,同时临床牙周参数也在恶化。第一阶段牙周治疗后,唾液和血清中的 IL-1β 和 MDA 水平都明显下降,同时临床参数也有所改善。此外,经过六周的洁治和根管治疗后,我们的数据显示唾液中的 IL-1β 和 MDA 水平与 PPD 和 CAL 之间存在很强的正相关关系。CP 患者治疗后临床参数和生物标志物水平显著下降,这证明 SRP 治疗能有效控制牙周健康。这表明唾液中的 IL-1β 和 MDA 可能是显示牙周病严重程度和治疗效果的有用生物标志物。
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引用次数: 0
Evaluation of uric acid levels and other biochemical parameters among Gout patients with Ketogenic diet in Erbil province. 评估埃尔比勒省采用生酮饮食的痛风患者的尿酸水平和其他生化指标。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.6
Kwestan R Muhammad

Gout is a systemic disorder that occurs due to an accumulation of uric acid crystals in the tissues. The association between the Ketogenic diet and uric acid concentration has been poorly established. This study aims to evaluate and assess the association of Serum uric acid and other variables with ketogenic diet among Gout patients in comparison with Healthy control subjects. A case-control observational study. Subjects were grouped into Group I (Gout patients-103 individuals) and Group II (healthy Subjects-55 individuals). Parameters of Serum creatinine, blood urea, and uric acid were assessed for both groups. The study population included 51.3% of male and 48.7% of female participants, with an age range of 20-74, with a mean of 35.72±13.69 years old. Ketogenic and meat-rich diet as strong risk factors for Gout were higher among all case groups (45.6%) and (92.2%), respectively. The Pearson's correlation coefficient of serum uric acid with other variables showed that the relation between serum uric acid with age, and weight among gout patients was found to be a weakly positive correlation and statistically significant (r=0.24, P= 0.013), and (r=0.22, P=0.026) respectively. This prospective study confirms that a ketogenic diet and a diet rich in meat have been associated with an increased incidence of gout.  Indeed, results have shown that the ketogenic diet might have an increasing effect on serum uric acid. The frequencies of comorbidities have been constantly shown to be increased in gout.

痛风是一种全身性疾病,是由于尿酸结晶在组织中积聚而引起的。生酮饮食与尿酸浓度之间的关系尚未得到充分证实。本研究旨在评估痛风患者血清尿酸及其他变量与生酮饮食的关联,并与健康对照组进行比较。这是一项病例对照观察研究。受试者分为第一组(痛风患者-103 人)和第二组(健康受试者-55 人)。对两组受试者的血清肌酐、血尿素和尿酸参数进行了评估。研究对象中男性占 51.3%,女性占 48.7%,年龄在 20-74 岁之间,平均年龄为(35.72±13.69)岁。在所有病例组中,生酮饮食和富含肉类的饮食是痛风的高危因素,分别占 45.6% 和 92.2%。血清尿酸与其他变量的皮尔逊相关系数显示,痛风患者的血清尿酸与年龄和体重之间呈弱正相关,分别为(r=0.24,P=0.013)和(r=0.22,P=0.026),具有统计学意义。这项前瞻性研究证实,生酮饮食和富含肉类的饮食与痛风发病率增加有关。 事实上,研究结果表明,生酮饮食可能会增加血清尿酸。在痛风患者中,合并症的发生率不断增加。
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引用次数: 0
Incidence of catheter-association bloodstream infection among hemodialysis patients at Erbil Teaching Hospital. 埃尔比勒教学医院血液透析患者中导管相关血流感染的发生率。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.23
Majeed Hasan Mahmood

The study objectives were to analyze catheter-associated bloodstream infection (CABSI) risk factors in chronic kidney disease on regular hemodialysis and identify the bacterial species responsible for this by molecular analysis. This research was conducted in Erbil Teaching Hospital-Dialysis Unit in Erbil City-Kurdistan Region-Iraq from January to June 2024. It has been performed on 100 hemodialysis samples from both males and females. The investigation showed that the prevalence of CABSI among hemodialysis patients was 44 (44%) out of 100 (100%). The highest percentage of patients were aged between 60-69 years (32%, OR= 0.9, 95%CI [0.1-2.4], P< 0.001) and also male (66%, OR=2.7, 95%CI [0.9-9.4], P< 0.032). Additionally, the patients with Diabetes Mellitus were 70%, (OR= 6.3, 95%CI [0.3-10.4], P< 0.031), and with hypertension was 92%, (OR= 3.1, 95%CI [0.21-5.4], P<0.02.  However, the dialysis duration of most patients was between 1-3 months (60%, OR=0.1, 95%CI [0.1-3.2], P<0.006) and the majority used two catheters (52%, OR= 0.6, 95%CI [0.1-3.2], P<0.012). The most common pathogens identified were Staphylococcus epidermis (44 cases, 100%), Pseudomonas aeruginosa (29 cases, 66%), and, Acinetobacter baumanni (24 cases, 55%). Thirteen bacterial species were recorded in the NCBI GenBank database. The phylogenetic tree demonstrated the distribution and relationship between these bacteria in hemodialysis patients. It showed that the bacterial species were closely related. To lower the risk of catheter-associated bloodstream infection, medical staff should actively develop countermeasures and gain a thorough understanding of the risk factors, which include age, diabetes, length of catheterization, and catheterization site.

研究目的是分析定期进行血液透析的慢性肾脏病患者导管相关血流感染(CABSI)的风险因素,并通过分子分析确定造成这种感染的细菌种类。本研究于 2024 年 1 月至 6 月在伊拉克埃尔比勒市库尔德斯坦地区的埃尔比勒教学医院透析室进行。研究对 100 份男性和女性血液透析样本进行了分析。调查显示,在 100 名血液透析患者(100%)中,CABSI 患病率为 44(44%)。年龄在 60-69 岁之间的患者比例最高(32%,OR= 0.9,95%CI [0.1-2.4],P< 0.001),男性患者比例也最高(66%,OR= 2.7,95%CI [0.9-9.4],P< 0.032)。此外,糖尿病患者占 70%(OR=6.3,95%CI [0.3-10.4],P< 0.031),高血压患者占 92%(OR=3.1,95%CI [0.21-5.4],P< 0.001)。
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引用次数: 0
Mesenchymal stem cells treated with Interleukin-1 beta for mediation of an inflammatory response in human tissues. 用白细胞介素-1β处理间充质干细胞,以调节人体组织的炎症反应。
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.5
Mansour Alsharidah, Mona Elsafadi, Osamah Al Rugaie, Amer Mahmood, Khalid M Mohany, Khalid A Al-Regaiey, Khaleel I Alyahya, Abdel-Moneim Hafez Abdel-Moneim, Abir El Sadik, Mohammad Abumaree

The present study examined the functional activities of the human bone marrow mesenchymal stem cells (hBM-MSCs) under the effects of various concentrations of the inflammatory mediator interleukin 1 beta (IL-1β). The effects of IL-1β on the functional properties of hBM-MSCs were measured using functional assays (adhesion, proliferation, and migration). hBM-MSCs expressions of colony-stimulating factors 1 and 2 (CSF1, CSF2), C-C chemokine receptor type 2 (CCR2), C-X-C chemokine receptor type 1 and 3 (CXCL1, CXCL3), were examined using real-time polymerase chain reaction (RT‒PCR). The pro-inflammatory cytokine IL-1β did not disrupt hBM-MSCs adhesion, but it improved proliferation and migration only up to 50 ng/ml. However, in response to 100 ng/ml IL-1β, cell growth, proliferation, and migration were reduced significantly. The expression of CSF1, CCR2, CXCL3, and IL-1β genes increased with the increase in the concentration of IL-1β. CSF2 and CXCL1 gene expression increased in the 50ng/ml group compared with the 10ng/ml group to be higher than the control group in the 100ng/ml IL-1β group which might facilitate the differentiation, and homing of MSCs to the site of injury and augment their activities in the inflamed microenvironment. The study corroborates the advantages of prior stimulation of mesenchymal stem cells (MSCs) with the cytokine IL-1β, demonstrating an upregulation of key chemokines and cytokines. This upregulation potentially enhances MSCs' ability to differentiate and migrate to injury sites, while also augmenting their functional role within an inflamed microenvironment, thereby amplifying their therapeutic potential.

本研究考察了人骨髓间充质干细胞(hBM-MSCs)在不同浓度的炎症介质白细胞介素1β(IL-1β)作用下的功能活动。采用实时聚合酶链反应(RT-PCR)检测了集落刺激因子1和2(CSF1、CSF2)、C-C趋化因子受体2型(CCR2)、C-X-C趋化因子受体1和3型(CXCL1、CXCL3)的表达。促炎细胞因子IL-1β不会破坏hBM-间充质干细胞的粘附性,但只能在50 ng/ml以下改善其增殖和迁移。然而,在 100 ng/ml IL-1β 的作用下,细胞的生长、增殖和迁移显著降低。随着 IL-1β 浓度的增加,CSF1、CCR2、CXCL3 和 IL-1β 基因的表达量也增加。与10ng/ml组相比,50ng/ml组的CSF2和CXCL1基因表达量增加,100ng/ml IL-1β组的CSF2和CXCL1基因表达量高于对照组。该研究证实了间充质干细胞(MSCs)事先接受细胞因子IL-1β刺激的优势,显示了关键趋化因子和细胞因子的上调。这种上调可能会增强间充质干细胞分化和迁移到损伤部位的能力,同时也会增强其在炎症微环境中的功能作用,从而扩大其治疗潜力。
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引用次数: 0
Evaluation of TP53TG1 and PANDA lncRNAs expression in association with adjuvant chemotherapy response in the peripheral blood of invasive ductal carcinoma patients. 评估浸润性导管癌患者外周血中TP53TG1和PANDA lncRNAs表达与辅助化疗反应的关系
IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-24 DOI: 10.14715/cmb/2024.70.10.9
Mostafa Noroozpoor, Hossein Mozdarani, Roghayeh Rahbar Parvaneh, Marzieh Lashkari

Breast cancer is the most common malignancy in women. Breast cancer, the second leading cause of cancer deaths, affects 2.1 million women each year and is estimated to have killed 627,000 women worldwide in 2018. Unfortunately, the age of onset of this cancer in our country IRAN is about 10 years lower than the global average and is close to 45 years. Chemotherapy is one of the basic treatments for cancer. Predicting the benefits of chemotherapy is challenging. Studies are now underway to use gene expression tests to pinpoint patients who are most likely to benefit from adjuvant chemotherapy. In the present study, the expression of two long non-coding RNAs TP53TG1 and PANDA in the blood of breast cancer patients before and after receiving chemotherapy compared with this amount in the blood of normal people using Real-Time RT PCR technique to find a meaningful relationship ¬ Compared statistically. Compared to normal samples, the expression level of TP53TG1 in the blood of patients was reduced. Although it was not statistically significant. Its expression also increased after receiving chemotherapy. Compared to normal samples, the expression of PANDA in the blood of patients was increased, which was statistically significant. Also, its expression decreased after receiving chemotherapy. These findings suggest that PANDA and TP53TG1 expression levels may be possible markers associated with tumorigenesis and may also be considered as possible indicators of response to chemotherapy.

乳腺癌是女性最常见的恶性肿瘤。乳腺癌是癌症死亡的第二大原因,每年影响 210 万名妇女,估计 2018 年全球有 62.7 万名妇女死于乳腺癌。不幸的是,在我国伊朗,这种癌症的发病年龄比全球平均水平低约 10 岁,接近 45 岁。化疗是癌症的基本治疗方法之一。预测化疗的疗效具有挑战性。目前正在研究利用基因表达检测来确定哪些患者最有可能从辅助化疗中获益。本研究利用实时 RT PCR 技术,将乳腺癌患者接受化疗前后血液中两种长非编码 RNA TP53TG1 和 PANDA 的表达量与正常人血液中的表达量进行了比较,以找到有意义的关系¬并进行统计。与正常样本相比,患者血液中 TP53TG1 的表达水平有所降低。虽然没有统计学意义。接受化疗后,其表达量也有所增加。与正常样本相比,患者血液中 PANDA 的表达水平升高,有统计学意义。此外,在接受化疗后,其表达也有所下降。这些研究结果表明,PANDA和TP53TG1的表达水平可能是与肿瘤发生相关的标志物,也可被视为化疗反应的可能指标。
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