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Determination of vitamin D, iron and n-3 fatty acids in adolescents with different eating habits. 不同饮食习惯青少年维生素D、铁和n-3脂肪酸的测定。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7048
Martina Valachovičová, Zuzana Slezáková, Jarmila Kristová, Vladimír Urbánek

Objectives: Proper eating habits ensure human health, so it is important to eat a balanced diet and learn the basics of proper eating from an early age. Adolescence is considered very important period for maintaining good health in terms of nutrition.

Methods: In the study, we monitored the eating habits of 182 adolescents aged 14-19 years with different eating habits (73 vegetarians and 109 nonvegetarians) using a questionnaire on nutrition and determination of selected blood parameters - vitamin D, iron and n-3fatty acids.

Results: Insufficient levels of vitamin D were determined in the whole group of adolescents, regardless of eating habits. Low iron concentrations negatively affect the biosynthesis of long-chain n-3 fatty acids, which was also reflected in our adolescents with alternative diets.

Conclusions: Based on the obtained results, it is necessary to place emphasis on the composition of the diet in adolescent age.

目的:适当的饮食习惯确保人体健康,所以饮食均衡和从小学习适当饮食的基本知识是很重要的。就营养而言,青春期被认为是保持健康的重要时期。方法:对182名14-19岁不同饮食习惯的青少年(73名素食者和109名非素食者)进行营养问卷调查,并测定维生素D、铁和n-3脂肪酸等血液指标。结果:无论饮食习惯如何,所有青少年都存在维生素D水平不足的问题。低铁浓度对长链n-3脂肪酸的生物合成产生负面影响,这也反映在我们的青少年替代饮食中。结论:基于上述结果,有必要重视青少年期饮食的组成。
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引用次数: 0
Risk factors for eating disorders among Chinese and international university students: a comparative cross-sectional study. 中国和国际大学生饮食失调的危险因素:一项比较横断面研究。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a6998
Areeba Yaqoob, Iqra Majeed, Hasnain Khalid, Shakeel Hussain, Muhammad Hamza Shahid, Fatima Majeed, Obaid-Ul- Hassan, Ijaz-Ul- Haq, Feng Qing

Objective: The aim of the study was to investigate the prevalence of eating disorders (EDs) and their association with socio-demographic and behavioural factors among university students.

Methods: A cross-sectional study among university students (Chinese and international) in Nanjing, China. We collected the data from 877 students, of which 811 were eligible for this study. They submitted a self-administered questionnaire (Eating Disorder Examination Questionnaire 6 (EDE-Q6) related to socio-demographic, health variables and lifestyle factors. Data were assessed with the help of SPSS software.

Results: A total of 401 Chinese and 410 international university students (49.44% vs. 50.55%) participated in this study. Binary logistic regression showed that young female adults of 18~25 years of age had more risk of developing eating disorders. Higher body mass index (BMI), such as overweight and obesity, were more influential risk factors (p < 0.001) for eating disorders. The significant risks (p < 0.001) EDs were found in students who were athletes, physically active, and involved in various extra-curricular activities. Alcohol and smoking were significant risk factors associated with eating disorders.

Conclusion: The results indicated higher risks of eating disorders followed by objective binge eating and compensatory behaviour. In this scenario, early assessment and treatment are necessary to reduce the burden of eating disorders and to promote good nutritional practices among university students.

目的:调查大学生饮食失调(EDs)的患病率及其与社会人口学和行为因素的关系。方法:采用横断面调查方法,对南京地区在校大学生进行调查。我们收集了877名学生的数据,其中811名符合本研究的条件。他们提交了一份与社会人口学、健康变量和生活方式因素相关的自我管理问卷(饮食失调检查问卷6 (EDE-Q6))。采用SPSS软件对数据进行评估。结果:共有401名中国大学生和410名国际大学生(49.44%对50.55%)参与了本研究。二元logistic回归分析显示,18~25岁的年轻成年女性发生饮食失调的风险较高。较高的身体质量指数(BMI),如超重和肥胖,是饮食失调更有影响的危险因素(p < 0.001)。在运动员、身体活跃、参加各种课外活动的学生中发现了显著的EDs风险(p < 0.001)。酒精和吸烟是与饮食失调相关的重要危险因素。结论:结果表明饮食失调的危险性较高,其次是客观暴食和代偿行为。在这种情况下,早期评估和治疗是必要的,以减轻饮食失调的负担,并在大学生中促进良好的营养习惯。
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引用次数: 2
Predictors of methamphetamine use in disadvantaged neighbourhoods in Czechia. 捷克弱势社区使用甲基苯丙胺的预测因素。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7121
Benjamin Petruželka, Petr Kupka, Václav Walach

Objective: This study aims to identify predictors of the lifetime prevalence of methamphetamine use in the population of Czech disadvantaged neighbourhoods.

Methods: Using data from a face-to-face representative survey, two types of analysis were performed. A bivariate analysis (unadjusted odds ratios estimated with logistic regression) was conducted to determine the relationship with a dependent variable (lifetime prevalence of methamphetamine use). Subsequently, three multivariate binomial logistic regression models (socio-demographic and socioeconomic status, incarceration and victimization, mobility and space) were conducted to control for the influence of other variables.

Results: In a series of multinomial logit models, we have found the following predictors to be significantly associated with lifetime prevalence of methamphetamine use: age, gender, Roma ethnicity, net monthly household income, unstable housing, lifetime experience with incarceration, lifetime experience with discrimination, urban-rural divide, and index of rural peripheralization.

Conclusions: The results suggest that methamphetamine users are multidimensionally disadvantaged and therefore constitute a vulnerable group with specific needs. This should be considered when designing services and policies targeting methamphetamine use in disadvantaged neighbourhoods.

目的:本研究旨在确定捷克弱势社区人口中甲基苯丙胺使用终生患病率的预测因素。方法:采用面对面代表性调查数据,进行两类分析。进行双变量分析(用逻辑回归估计未调整的优势比)以确定与因变量(一生中使用甲基苯丙胺的流行率)的关系。随后,采用三种多元二项逻辑回归模型(社会人口和社会经济地位、监禁和受害、流动性和空间)来控制其他变量的影响。结果:在一系列的多项logit模型中,我们发现以下预测因素与甲基安非他命终生使用的患病率显著相关:年龄、性别、罗姆民族、家庭月净收入、不稳定住房、终身监禁经历、终身歧视经历、城乡差距和农村外围化指数。结论:结果表明,甲基苯丙胺使用者在多方面处于不利地位,因此构成具有特定需求的弱势群体。在设计针对弱势社区使用甲基苯丙胺的服务和政策时,应考虑到这一点。
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引用次数: 0
Hepatitis B immunization data of patients living with HIV/AIDS: a multi-centre study. HIV/AIDS患者的乙肝免疫数据:一项多中心研究
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7300
Servet Öztürk, Ayşe Serra Özel, Pinar Ergen, Seniha Şenbayrak, Canan Ağalar

Objectives: Human immunodeficiency virus (HIV) and hepatitis B virus (HBV) are the two leading viruses that cause the greatest number of virus-related morbidities in the world. HIV/HBV coinfection is correlated with high morbidity and mortality. For this particular reason hepatitis B vaccination is crucial for people living with HIV.

Methods: Patients who are being followed-up for HIV/AIDS and who have received a hepatitis B vaccine in 4 HIV clinics over a 5-year time period have been studied. Our multi-centered, retrospective, cross-sectional and observational study investigates factors that affect hepatitis B vaccination immune response of individuals living with HIV. The patients have been studied for the parameters such as age, sex, CD4 count at the time of diagnosis or vaccination, HIV-RNA levels, comorbidities, vaccine dosage, success of immunization after vaccination, and the demographics of the patients who have and have not developed immunity.

Results: Of 645 patients that are being followed-up in our clinics, 158 received hepatitis B vaccine; 39 of these 158 patients have been excluded from the study because they did not fulfil the inclusion criteria. Finally, 119 patients were evaluated in the study, 17 of the patients (14.3%) were females and 102 (85.7%) were males. The median age was 41.11 ± 10.09 (min-max: 18-75). Twenty-three of the patients (19.3%) were at the stage of AIDS during diagnosis while 80.7% were at the stage of HIV infection. Ninety-one of the patients (76.5%) have been administered a single dose hepatitis B vaccine on the standard 0, 1st, 6th month vaccination schedule, whereas 23.5% were administered a double dose on the same vaccination schedule. When further evaluated to find whether the patient was able to develop sufficient immunity (anti-HBs ≥ 10), it was found that the immune response was statistically significantly higher in the patients whose CD4 count was greater than 200 at the time of the first diagnosis and vaccination (p = 0.05 and p = 0.001, respectively). The patients have also been evaluated according to the number of doses they received (1 vs. 2). The immune response of the patients who received two doses was statistically significantly higher (p = 0.041).

Conclusion: We can conclude that in the patients with CD4 count less than 200 at the time of their diagnosis and vaccination a high dose recombinant hepatitis B vaccine should definitely be administered as the normal dose and higher dose have similar side effect profiles and the higher dose provides greater immunity.

目的:人类免疫缺陷病毒(HIV)和乙型肝炎病毒(HBV)是世界上导致最多病毒相关疾病的两种主要病毒。HIV/HBV合并感染与高发病率和死亡率相关。由于这一特殊原因,乙肝疫苗接种对艾滋病毒感染者至关重要。方法:对5年期间在4家HIV诊所接受HIV/AIDS随访和乙肝疫苗接种的患者进行研究。我们的多中心、回顾性、横断面和观察性研究调查了影响HIV感染者接种乙肝疫苗免疫反应的因素。研究患者的参数包括年龄、性别、诊断或接种时的CD4计数、HIV-RNA水平、合并症、疫苗剂量、疫苗接种后的免疫成功率以及具有和未产生免疫的患者的人口统计数据。结果:645例患者中,158例接受了乙肝疫苗接种;这158例患者中有39例因不符合纳入标准而被排除在研究之外。最终纳入119例患者,其中女性17例(14.3%),男性102例(85.7%)。中位年龄41.11±10.09岁(最小-最大18-75岁)。其中23例(19.3%)患者在诊断时处于艾滋病阶段,80.7%的患者处于HIV感染阶段。91例(76.5%)患者按标准0、1、6个月接种单剂乙肝疫苗,23.5%的患者按相同接种计划接种双剂乙肝疫苗。当进一步评估患者是否能够产生足够的免疫(anti-HBs≥10)时,发现首次诊断和接种时CD4计数大于200的患者的免疫应答具有统计学意义(p = 0.05和p = 0.001)。根据患者接受的剂量数(1 vs. 2)对患者进行了评估。接受两次剂量的患者的免疫反应具有统计学意义上的更高(p = 0.041)。结论:在诊断和接种时CD4计数小于200的患者应接种高剂量重组乙肝疫苗,因为正常剂量和高剂量副作用相似,且高剂量免疫效果更好。
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引用次数: 0
Foodborne streptococcal tonsillopharyngitis outbreak in a hospital. 一家医院爆发食源性链球菌扁桃体咽炎。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a6027
Şemsi Nur Karabela, Sevtap Şenoğlu, Özlem Altuntaş Aydin, Kürşad Nuri Baydili, Özlem Aksu, Kadriye Kart Yaşar

Objective: Group A beta-haemolytic streptococci (GAS), which are responsible for most cases of acute bacterial tonsillopharyngitis, are transmitted from person to person and may rarely cause foodborne outbreaks. This study aims to report the epidemic caused by GAS in our hospital and to draw attention to the explosive outbreaks of the bacteria.

Methods: Acute tonsillopharyngitis was seen in 201 of 450 hospital employees who ate in the hospital cafeteria on 4-5 June 2015.

Results: GAS was detected in 106 (68%) of 157 cases and in 40 (63.5%) of 62 throat culture samples. The attack rate was 44.7%. The most suspected source of the outbreak was a food handler who had been showing signs of streptococcal tonsillopharyngitis for six days, and perhaps the food prepared by these staff.

Conclusion: It should not be forgotten that GAS can cause explosive outbreaks by infecting food through hand lesions or mouth secretions of food service personnel.

目的:A群溶血链球菌(GAS)是大多数急性细菌性扁桃体咽炎病例的病因,可在人与人之间传播,很少引起食源性暴发。本研究的目的是报告我院由气体引起的疫情,引起人们对细菌爆发性爆发的重视。方法:2015年6月4日至5日在医院食堂就餐的450名医院员工中,201人出现急性扁桃体咽炎。结果:157例咽喉培养标本中有106例(68%)检出GAS, 62例中有40例(63.5%)检出GAS。发病率为44.7%。最可疑的疫情来源是一名食品处理人员,他已出现6天链球菌扁桃体咽炎的迹象,可能是这些工作人员准备的食物。结论:不应忘记,气体可通过食品服务人员的手部病变或口腔分泌物感染食物,引起爆炸性疫情。
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引用次数: 0
Occupational diseases arising in the area of the Ministry of Defence in the Czech Republic and their relationship to work categorization. 捷克共和国国防部辖区内发生的职业病及其与工作分类的关系。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7235
Blanka Kupsová, Vladimír Pavlík, Jan M Horáček, Václav Šafka, Petr Lašák, Jana Fajfrová, Michaela Husárová, Karin Boušová, Milan Tuček

Objective: The article deals with occupational health protection and identification of health risks in the work environment of the Ministry of Defence (MoD) of the Czech Republic (CR). It focuses on the assessment of the incidence of occupational diseases (OD) in high-risk and risk-free occupational categories in the years 2010-2019 and compares them with data from the civilian sector. It identifies the differences between military staff and civilian employees of the MoD.

Methods: From the records of OD at the Department of Occupational Diseases of the Central Military Hospital in Prague, the data on acknowledged OD from the years 2010 to 2019 were obtained and then a retrospective analysis focusing on the classification of work at risk was performed. The obtained data were compared with the data from the Czech National Registry of Occupational Diseases (NROD), which are published annually by the National Institute of Public Health.

Results: In the years under review, 191 OD were confirmed at the area of MoD, 26% of all OD occurred in employees classified in the occupational risk category. Compared with the data in the NROD, where 50% of OD were found to have been caused by high-risk work, the incidence of OD caused by high-risk work in professional soldiers is lower. Only 1.6% of all OD occurred in professional soldiers whose work was classified as high-risk one. In civilian employees of MoD 24.6% of all OD were connected with high-risk work. On the contrary, the proportion of OD occurring in professional soldiers working in risk-free categories was 57.6%, in civilian employees of MoD was the ratio much lower - 16.2%.

Conclusion: Work at the Ministry of Defence was not adequately categorized, therefore, in 2020 a new categorization of work was introduced, which together with preventive measures could contribute to reducing the incidence of OD at the Ministry of Defence.

目的:本文涉及捷克共和国国防部(国防部)工作环境中的职业健康保护和健康风险识别。该报告侧重于评估2010-2019年高风险和无风险职业类别的职业病发病率,并将其与民用部门的数据进行比较。方法:从布拉格中央军事医院职业病科2010 - 2019年的OD记录中获取已确认的OD数据,并以风险工作分类为重点进行回顾性分析。将获得的数据与捷克国家职业病登记处(NROD)的数据进行了比较,后者每年由国家公共卫生研究所公布。结果:回顾年度,在MoD区域确诊OD 191例,其中26%的OD发生在职业风险类员工中。与NROD的数据相比,其中50%的OD被发现是由高风险工作引起的,职业军人中高风险工作引起的OD发生率较低。只有1.6%的OD发生在职业军人中,他们的工作被列为高危工作。在国防部文职人员中,从事高危工作的占24.6%。相反,在无风险类别工作的专业士兵中,发生OD的比例为57.6%,在国防部文职雇员中,这一比例要低得多,为16.2%。结论:国防部的工作分类不充分,因此,在2020年引入了新的工作分类,该分类与预防措施一起有助于减少国防部的工作过量发生率。
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引用次数: 0
The mechanism of spoilage of a bottled soft drink leading to health problem of consumer. 瓶装软饮料变质导致消费者健康问题的机理。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7388
Lenka Mayerová, František Kožíšek, Dana Baudišová, Kateřina Klánová, Barbora Hlaváčková

The case of spoilage of flavoured and sweetened soft drink occurred in the Czech Republic in 2019. After drinking it, the consumer was admitted to a hospital with sickness. The spoilage was caused by mould and an odorous substance, 1,3-pentadiene. The mechanism of food spoilage, with the formation of 1,3-pentadiene arising from the decarboxylation of sorbic acid and mould growth, is described. This could be the second case history reported worldwide of an allergic reaction to penicillin explaining how penicillin might get into the beverage. We hypothesise three possible causes of the health problem experienced with allergic reaction to penicillin or other mycotoxin produced by Penicillium mould as the most probable one.

2019年,捷克共和国发生了调味和加糖软饮料变质的案件。消费者喝了之后,生病住进了医院。腐坏是由霉菌和一种有气味的物质1,3-戊二烯引起的。描述了食品变质的机制,山梨酸脱羧和霉菌生长产生1,3-戊二烯。这可能是世界范围内报告的第二例青霉素过敏反应,解释了青霉素是如何进入饮料的。我们假设三种可能的健康问题的原因经历青霉素或其他霉菌毒素产生的青霉菌霉菌过敏反应是最可能的一个。
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引用次数: 0
Parents' views and information status on childhood vaccines: which myths play a role. 父母对儿童疫苗的看法和信息现状:哪些误解起了作用。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a6946
Multehan Evran, Hayrunnisa Bekis Bozkurt

Objectives: The participation of families in childhood vaccination decreases slightly every year around the world. Parents arrive at a decision that vaccines are not safe for their children due to many sources of misinformation. The aim of this study was to investigate the relationship between vaccine hesitancy, vaccine knowledge status and socio-demographic characteristics of the children's parents.

Methods: In this cross-sectional study, 361 parents of children aged 5 years and under, who were admitted to our paediatric outpatient clinic, were included. The parents received scores between 0-20 points according to the correct answers they gave to the questions asked.

Results: Although all parents had a positive attitude towards vaccination, some myths, e.g. that vaccination could weaken the child's immune system because it contains heavy metals which could cause infertility and that complementary and alternative medicine could replace vaccination, are thought to be real by 1.7% to 34.6% of the parents.

Conclusions: Since the presence of misinformation may lead to vaccine hesitancy and incomplete vaccination, healthcare personnel have important duties and responsibilities for this group.

目的:在世界范围内,家庭参与儿童疫苗接种的人数每年都略有下降。由于许多来源的错误信息,父母得出结论,疫苗对他们的孩子不安全。本研究旨在探讨疫苗犹豫、疫苗知识状况与儿童父母社会人口学特征的关系。方法:在这个横断面研究中,包括361名5岁及以下儿童的父母,他们在我们的儿科门诊就诊。根据父母对问题的正确回答,他们得到0-20分的分数。结果:尽管所有家长对接种疫苗持积极态度,但1.7% ~ 34.6%的家长认为接种疫苗会削弱孩子的免疫系统,因为疫苗中含有可导致不孕的重金属,补充和替代药物可以取代疫苗接种。结论:由于错误信息的存在可能导致疫苗犹豫和疫苗接种不完整,卫生保健人员对这一群体负有重要的义务和责任。
{"title":"Parents' views and information status on childhood vaccines: which myths play a role.","authors":"Multehan Evran,&nbsp;Hayrunnisa Bekis Bozkurt","doi":"10.21101/cejph.a6946","DOIUrl":"https://doi.org/10.21101/cejph.a6946","url":null,"abstract":"<p><strong>Objectives: </strong>The participation of families in childhood vaccination decreases slightly every year around the world. Parents arrive at a decision that vaccines are not safe for their children due to many sources of misinformation. The aim of this study was to investigate the relationship between vaccine hesitancy, vaccine knowledge status and socio-demographic characteristics of the children's parents.</p><p><strong>Methods: </strong>In this cross-sectional study, 361 parents of children aged 5 years and under, who were admitted to our paediatric outpatient clinic, were included. The parents received scores between 0-20 points according to the correct answers they gave to the questions asked.</p><p><strong>Results: </strong>Although all parents had a positive attitude towards vaccination, some myths, e.g. that vaccination could weaken the child's immune system because it contains heavy metals which could cause infertility and that complementary and alternative medicine could replace vaccination, are thought to be real by 1.7% to 34.6% of the parents.</p><p><strong>Conclusions: </strong>Since the presence of misinformation may lead to vaccine hesitancy and incomplete vaccination, healthcare personnel have important duties and responsibilities for this group.</p>","PeriodicalId":9823,"journal":{"name":"Central European journal of public health","volume":"30 4","pages":"219-224"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10597554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Death from COVID-19 of a 57-year-old man refusing medical care and self-medicating with ivermectin. 一名57岁男子死于COVID-19,拒绝接受医疗护理并使用伊维菌素自我治疗。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7643
Robin Šín, Miroslav Kubiska

Since December 2019, the new SARS-CoV-2 coronavirus causes COVID-19 disease worldwide, which occurs mainly in unvaccinated elderly and polymorbid patients with a more severe course and increased risk of complications and death. Vaccination and specific therapy for the disease using mainly new antiviral drugs are the way to reduce the number of infected, hospitalized patients with a more severe course. We present a case report of an at-risk polymorbid 57-year-old man who refused vaccination and standard treatment for COVID-19 disease based on misinformation from the community. He self-treated himself with high dose of ivermectin. The patient died at home 14 days after the onset of symptoms.

自2019年12月以来,新型SARS-CoV-2冠状病毒在全球范围内引起COVID-19疾病,主要发生在未接种疫苗的老年人和多病患者中,病程更严重,并发症和死亡风险增加。疫苗接种和主要使用新的抗病毒药物对疾病进行特异性治疗是减少感染人数的方法,住院患者的病程更严重。我们报告了一名高危多病57岁男性的病例报告,他根据社区的错误信息拒绝接种COVID-19疫苗和标准治疗。他用大剂量的伊维菌素自我治疗。患者在出现症状14天后在家中死亡。
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引用次数: 1
Hand disinfectants and their activity against clinical isolates of Bordetella pertussis. 手部消毒剂对百日咳杆菌临床分离株的活性研究。
IF 1.2 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Pub Date : 2022-12-01 DOI: 10.21101/cejph.a7141
Petra Uttlová, Jan Urban

Objectives: The aim of the study was to investigate possible emergence of resistance to disinfectants in Bordetella pertussis strains isolated from patients with whooping cough in the Czech Republic in 2014 and 2015.

Methods: In an EN1500-based study, clean and dry fingertips of volunteers were always contaminated with one of the two clinical isolates of B. pertussis. Clinical isolates of B. pertussis were obtained from the National Reference Laboratory for Pertussis and Diphtheria, National Institute of Public Health (NIPH), Prague, Czech Republic. Dry and contaminated fingertips were immersed in 10 ml medium and then rubbed with the fingers for 1 minute. After that, the hands were treated with isopropanol 60% v/v or tested products, and then the fingertips were rubbed again into 10 ml of pure medium for 1 minute. The suspensions obtained were immediately diluted and plated on charcoal medium.

Results: Ethanol-based product A and propanol-based product B showed bactericidal activity after 30 s of contact. The confidence interval limit for product A and B was 0.12 and 0.19, respectively. Quaternary ammonium compound-based product C was found to be ineffective after 30 s of contact. The confidence interval limit for product C was 0.62.

Conclusion: Products A and B were assessed as effective against clinical isolates of B. pertussis in accordance with EN 1500. Quaternary ammonium compound-based product C did not comply with the requirements of EN 1500.

目的:本研究的目的是调查2014年和2015年从捷克共和国百日咳患者中分离的百日咳博德泰拉菌株对消毒剂可能出现的耐药性。方法:在一项基于en1500的研究中,志愿者的清洁和干燥的指尖总是被两种临床分离株百日咳中的一种污染。百日咳临床分离株来自捷克共和国布拉格国家公共卫生研究所(NIPH)百日咳和白喉国家参考实验室。将干燥和污染的指尖浸入10ml培养基中,然后用手指摩擦1分钟。之后,用60% v/v的异丙醇或被试产品处理双手,然后将指尖再次放入10 ml纯培养基中揉搓1分钟。得到的悬浮液立即稀释,并在木炭培养基上镀。结果:乙醇基产品A和丙醇基产品B在接触30 s后显示出杀菌活性。产品A和B的置信区间极限分别为0.12和0.19。季铵盐基产品C在接触30 s后失效。产品C的置信区间限为0.62。结论:产品A和B对临床分离株百日咳有效,符合en1500标准。季铵化合物基产品C不符合en1500的要求。
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引用次数: 0
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Central European journal of public health
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