Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.006
Zhijin Zhao, Ping Yang, Z. Ji, Wan-yuan He
Objective To explore the characteristics and differences of micro-perfusion between patients after transplant kidney with stable renal function, acute rejection and chronic rejection by contrast-enhanced ultrasound (CEUS) quantifying. Methods Thirty-three patients with stable renal function, 27 patients with acute rejection and 14 patients with chronic rejection were enrolled. The perfusion parameters of region of interest in renal cortex and medulla were obtained by CEUS offline data quantitative analysis software. The perfusion parameters in stable renal function group were compared with those in acute rejection and chronic rejection groups; furthermore, the value of color Doppler and CEUS in the diagnosis of chronic rejection was explored and compared. Results As for resistance index RI on color Doppler, it made no statistical difference among three groups (P>0.05). The perfusion parameters derived from CEUS quantitative analysis and Color Doppler parameter resistance index (RI) were not statistically different among renal function stabilization, acute rejection and chronic rejection groups (P>0.05); as compared with stable renal function group, there were marked reductions in peak intensity of cortex (PIc), peak intensity of medulla (PIm), ascending slope of cortex (a3c), ascending slope of medulla (a3m) and area under the curve of cortex (AUCc) in chronic rejection group. And mean transit time of cortex (MTTc) and mean transit time of medulla (MTTm) became significantly prolonged (P<0.05). Except for a3m, PIc and PIm were not significantly different between stable renal function and acute rejection groups (P<0.05). As compared with acute rejection group, MTTc and MTTm were significantly prolonged in chronic rejection group while AUCm and PIm declined markedly (P<0.05). When PIm<26.7dB, the diagnostic efficiency of chronic rejection was the highest and the sensitivity and specificity were 85.7% and 72.7% respectively. Conclusions As compared with color Doppler, CEUS quantitative parameters based on S-G filter theory can evaluate the micro-perfusion of transplant kidney more precisely and may provide diagnostic clue for chronic rejection. Key words: Kidney transplantation; Ultrasound contrast; Rejection
{"title":"Value of quantitative contrast-enhanced ultrasound based on S-G filter theory in the diagnosis of chronic rejection of transplant kidney","authors":"Zhijin Zhao, Ping Yang, Z. Ji, Wan-yuan He","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.006","url":null,"abstract":"Objective \u0000To explore the characteristics and differences of micro-perfusion between patients after transplant kidney with stable renal function, acute rejection and chronic rejection by contrast-enhanced ultrasound (CEUS) quantifying. \u0000 \u0000 \u0000Methods \u0000Thirty-three patients with stable renal function, 27 patients with acute rejection and 14 patients with chronic rejection were enrolled. The perfusion parameters of region of interest in renal cortex and medulla were obtained by CEUS offline data quantitative analysis software. The perfusion parameters in stable renal function group were compared with those in acute rejection and chronic rejection groups; furthermore, the value of color Doppler and CEUS in the diagnosis of chronic rejection was explored and compared. \u0000 \u0000 \u0000Results \u0000As for resistance index RI on color Doppler, it made no statistical difference among three groups (P>0.05). The perfusion parameters derived from CEUS quantitative analysis and Color Doppler parameter resistance index (RI) were not statistically different among renal function stabilization, acute rejection and chronic rejection groups (P>0.05); as compared with stable renal function group, there were marked reductions in peak intensity of cortex (PIc), peak intensity of medulla (PIm), ascending slope of cortex (a3c), ascending slope of medulla (a3m) and area under the curve of cortex (AUCc) in chronic rejection group. And mean transit time of cortex (MTTc) and mean transit time of medulla (MTTm) became significantly prolonged (P<0.05). Except for a3m, PIc and PIm were not significantly different between stable renal function and acute rejection groups (P<0.05). As compared with acute rejection group, MTTc and MTTm were significantly prolonged in chronic rejection group while AUCm and PIm declined markedly (P<0.05). When PIm<26.7dB, the diagnostic efficiency of chronic rejection was the highest and the sensitivity and specificity were 85.7% and 72.7% respectively. \u0000 \u0000 \u0000Conclusions \u0000As compared with color Doppler, CEUS quantitative parameters based on S-G filter theory can evaluate the micro-perfusion of transplant kidney more precisely and may provide diagnostic clue for chronic rejection. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Ultrasound contrast; Rejection","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"13 1","pages":"215-218"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75129847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.010
Zhenkun Dong, Xinsheng Xie, Zhongxing Jiang, D. Wan, Jia Liu, Wenwen Ding, Haiqiong Wang, Fei He
Objective To observe the efficacy of maintenance treatment with decitabine and dasatinib after allogenic hematopoietic stem cell transplantation for myeloid sarcoma. Methods A 29-year-old male patient was diagnosed with abdominal myeloid sarcoma and acute myeloid leukemia with c-kit mutation and t(8; 21). Allogeneic hematopoietic stem cell transplantation was performed after inducted remission. The conditioning regimen was decitabine + FLAG + modified Bu/Cy. Prophylaxis of GVHD was performed with cyclosporine mycophenolate mofetil and short-term methotrexate. The patient received 11.73×108 mononucleated cells/kg and 17.59×106CD34+ cells/kg from donor. At Day 13 post-transplantation, neutrophils reached 0.5×109/L and platelet count was 20×109/L. Decitabine was prescribed since Day 50 post-transplantation monthly for 5 courses. And dasatinib was offered orally since Day 100 for 4 months. Results It was followed up to 16 months post-transplantation. There were no obvious abnormalities of bone marrow cytology, AML/ETO fusion gene quantification, cerebrospinal fluid or abdominal enhanced computed tomography (CT). Conclusions Hematopoietic stem cell transplantation is an effective treatment for myeloid sarcoma. Decitabine has some efficacy for myeloid sarcoma and it may be used for maintenance treatment after transplantation. Tyrosine kinase inhibitors reduce recurrence in myeloid sarcoma with c-kit mutation. The treatment of decitabine and dasatinib after allogeneic hematopoietic stem cell transplantation yield excellent outcomes. This is the first report in domestic and foreign literatures. Key words: Hematopoietic stem cell transplantation; Myeloid sarcoma; Allogene
目的观察异基因造血干细胞移植后地西他滨联合达沙替尼维持治疗髓系肉瘤的疗效。方法1例29岁男性腹腔髓系肉瘤合并急性髓系白血病合并c-kit突变和t(8;21)。诱导缓解后进行同种异体造血干细胞移植。调理方案为地西他滨+ FLAG +改性Bu/Cy。预防GVHD采用环孢素霉酚酸酯和短期甲氨蝶呤。患者分别接受供体提供的11.73×108单核细胞/kg和17.59×106CD34+细胞/kg。移植后第13天，中性粒细胞达到0.5×109/L，血小板计数20×109/L。从移植后第50天开始每月开地西他滨5个疗程。从第100天起口服达沙替尼，持续4个月。结果随访至移植后16个月。骨髓细胞学、AML/ETO融合基因定量、脑脊液及腹部增强CT检查均未见明显异常。结论造血干细胞移植是治疗髓系肉瘤的有效方法。地西他滨对髓系肉瘤有一定疗效，可用于移植后的维持治疗。酪氨酸激酶抑制剂可减少c-kit突变髓系肉瘤的复发。异基因造血干细胞移植后地西他滨和达沙替尼治疗效果良好。这在国内外文献中尚属首次报道。关键词:造血干细胞移植;髓系肉瘤;隐性基因
{"title":"Maintenance therapy after hematopoietic stem cell transplantation for abdominal myeloid sarcoma: a case report and literature review","authors":"Zhenkun Dong, Xinsheng Xie, Zhongxing Jiang, D. Wan, Jia Liu, Wenwen Ding, Haiqiong Wang, Fei He","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.010","url":null,"abstract":"Objective \u0000To observe the efficacy of maintenance treatment with decitabine and dasatinib after allogenic hematopoietic stem cell transplantation for myeloid sarcoma. \u0000 \u0000 \u0000Methods \u0000A 29-year-old male patient was diagnosed with abdominal myeloid sarcoma and acute myeloid leukemia with c-kit mutation and t(8; 21). Allogeneic hematopoietic stem cell transplantation was performed after inducted remission. The conditioning regimen was decitabine + FLAG + modified Bu/Cy. Prophylaxis of GVHD was performed with cyclosporine mycophenolate mofetil and short-term methotrexate. The patient received 11.73×108 mononucleated cells/kg and 17.59×106CD34+ cells/kg from donor. At Day 13 post-transplantation, neutrophils reached 0.5×109/L and platelet count was 20×109/L. Decitabine was prescribed since Day 50 post-transplantation monthly for 5 courses. And dasatinib was offered orally since Day 100 for 4 months. \u0000 \u0000 \u0000Results \u0000It was followed up to 16 months post-transplantation. There were no obvious abnormalities of bone marrow cytology, AML/ETO fusion gene quantification, cerebrospinal fluid or abdominal enhanced computed tomography (CT). \u0000 \u0000 \u0000Conclusions \u0000Hematopoietic stem cell transplantation is an effective treatment for myeloid sarcoma. Decitabine has some efficacy for myeloid sarcoma and it may be used for maintenance treatment after transplantation. Tyrosine kinase inhibitors reduce recurrence in myeloid sarcoma with c-kit mutation. The treatment of decitabine and dasatinib after allogeneic hematopoietic stem cell transplantation yield excellent outcomes. This is the first report in domestic and foreign literatures. \u0000 \u0000 \u0000Key words: \u0000Hematopoietic stem cell transplantation; Myeloid sarcoma; Allogene","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"103 1","pages":"237-240"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85382764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.005
Xuan-xuan Li, Wen-yi Liu, Jianhong Wang, Yang Zhao, Lina Zhu, Xin Wang, Yuan Guo, Yong Liu
Objective To explore the values of shear wave elastography (SWE) and ultrasonic grading in liver grafts from brain death donor (DBD). Methods Liver grafts of 39 DBD cases were examined preoperatively by ultrasonography (US) and SWE. Blood samples were tested preoperatively. Based upon early allograft function of liver recipients, the donors were classified into early allograft dysfunction (EAD) and early allograft function normal (non-EAD) groups. The SWE/US grading results of EAD group were compared with those of non-EAD group. Receiver operating curve (ROC) was employed for analyzing the diagnostic accuracy of SWE/US grading in EAD. Results The SWE value of EAD group was significantly higher than that of non-EAD group [(6.65±2.69) vs. (3.50±1.27) kPa, P<0.05]. Ultrasonic grading of EAD group was also significantly higher than that of non-EAD group (P<0.05). The area under ROC curve (AUROC) of SWE in EAD was 0.939, optimal cut-off value 4.56 kPa, AUROC of ultrasonic grading 0.806 (P=0.003) and optimal cut-off value level 3.5. Conclusions SWE quantifies fibrosis in liver grafts of DBD with a high diagnostic accuracy. There are significant correlations between EAD after liver transplantation and SWE value of liver grafts and ultrasonic grading. SWE and ultrasonic grading may improve the assessments of liver grafts of DBD. Key words: Liver Transplantation; Ultrasonography; Recovery of function
{"title":"Application values of shear wave elastography and ultrasonic grading in liver grafts from brain death donor","authors":"Xuan-xuan Li, Wen-yi Liu, Jianhong Wang, Yang Zhao, Lina Zhu, Xin Wang, Yuan Guo, Yong Liu","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.005","url":null,"abstract":"Objective \u0000To explore the values of shear wave elastography (SWE) and ultrasonic grading in liver grafts from brain death donor (DBD). \u0000 \u0000 \u0000Methods \u0000Liver grafts of 39 DBD cases were examined preoperatively by ultrasonography (US) and SWE. Blood samples were tested preoperatively. Based upon early allograft function of liver recipients, the donors were classified into early allograft dysfunction (EAD) and early allograft function normal (non-EAD) groups. The SWE/US grading results of EAD group were compared with those of non-EAD group. Receiver operating curve (ROC) was employed for analyzing the diagnostic accuracy of SWE/US grading in EAD. \u0000 \u0000 \u0000Results \u0000The SWE value of EAD group was significantly higher than that of non-EAD group [(6.65±2.69) vs. (3.50±1.27) kPa, P<0.05]. Ultrasonic grading of EAD group was also significantly higher than that of non-EAD group (P<0.05). The area under ROC curve (AUROC) of SWE in EAD was 0.939, optimal cut-off value 4.56 kPa, AUROC of ultrasonic grading 0.806 (P=0.003) and optimal cut-off value level 3.5. \u0000 \u0000 \u0000Conclusions \u0000SWE quantifies fibrosis in liver grafts of DBD with a high diagnostic accuracy. There are significant correlations between EAD after liver transplantation and SWE value of liver grafts and ultrasonic grading. SWE and ultrasonic grading may improve the assessments of liver grafts of DBD. \u0000 \u0000 \u0000Key words: \u0000Liver Transplantation; Ultrasonography; Recovery of function","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"1 1","pages":"211-214"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81810154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.011
Qiwen Yu, Hongwei Tang, Dongjing Yang, Wenzhi Guo, Jie Li
Objective To explore the role and mechanism of inducible nitric oxide synthase inhibitor 1 400W in alleviating ischemia-reperfusion injury of human intrahepatic bile duct epithelial cells. Methods Human intrahepatic bile duct epithelial cells (HIBEC) in logarithmic phase were inoculated into culture plate at an appropriate density. The samples were randomly divided into control group (group C), ischemia-reperfusion group (group I/R) and ischemia-reperfusion + 1 400W group (group I/R+ 1 400W). Group C was cultured routinely; cells in I/R and I/R+ 1 400W groups were placed in a three-gas incubator for 12h for simulating ischemia and then normal culture for 6h for simulating reperfusion. The I/R+ 1 400W group had a final concentration of 100 μmol/L of 1 400W before ischemia and hypoxia. After reperfusion, cells and culture medium were collected, CCK 8 was used for detecting cell vitality, microplate method for detecting the content of lactate dehydrogenase (LDH) in culture medium, AnnexinV-FITC/PI double stain for detecting apoptosis level, Western blot for analyzing the expressions of endoplasmic reticulum stress (ERS) related protein cysteinyl aspartic acid protease 12 (caspase-12), glucose regulatory protein 78 (GRP78) C/EBP homologous protein (CHOP) and inducible nitric oxide synthase (iNOS). Results As compared with group C, cell viability significantly decreased in I/R and I/R+ 1 400W groups (53.8%±2.3% vs.100%, 66.5%±2.8% vs.100%) (P<0.05) while LDH increased markedly in cell culture medium (287.4±9.0U/L vs 120.2±8.7U/L, 212.0±8.3U/L vs 120.2±8.7U/L) (P<0.05). Apoptosis accelerated markedly (41.5%±2.3% vs 5.2%±0.5%, 32.7%±1.8% vs 5.2%±0.5%) (P<0.05) and the expressions of caspase-12, GRP78, CHOP and iNOS spiked (P<0.05); as compared with I/R group, cell viability of I/R+ 1 400W group rose while LDH, apoptosis level, caspase-12, GRP78 and CHOP declined in cell culture medium (P<0.05). Conclusions 1 400W may alleviate ischemia-reperfusion injury of human intrahepatic bile duct epithelial cells and its mechanism may be correlated with a suppression of endoplasmic reticulum stress. Key words: Ischemia-reperfusion injury; Bile duct epithelial cells; Nitric oxide synthase
目的探讨诱导型一氧化氮合酶抑制剂1 400W在减轻人肝内胆管上皮细胞缺血再灌注损伤中的作用及机制。方法将对数期人肝内胆管上皮细胞(HIBEC)按适当密度接种于培养板。随机分为对照组(C组)、缺血再灌注组(I/R组)和缺血再灌注+ 1 400W组(I/R+ 1 400W组)。C组常规培养;将I/R组和I/R+ 1 400W组细胞置于三气培养箱中模拟缺血12h，然后正常培养6h模拟再灌注。I/R+ 1 400W组缺血缺氧前终浓度为100 μmol/L。再灌注后收集细胞和培养基，cck8检测细胞活力，微孔板法检测培养基中乳酸脱氢酶(LDH)含量，AnnexinV-FITC/PI双染色检测细胞凋亡水平，Western blot分析内质网应激(ERS)相关蛋白半胱氨酸天冬氨酸蛋白酶12 (caspase-12)的表达。葡萄糖调节蛋白78 (GRP78) C/EBP同源蛋白(CHOP)和诱导型一氧化氮合酶(iNOS)。结果与C组比较，I/R和I/R+ 1 400W组细胞活力显著降低(53.8%±2.3% vs.100%， 66.5%±2.8% vs.100%) (P<0.05)，细胞培养液LDH显著升高(287.4±9.0U/L vs 120.2±8.7U/L, 212.0±8.3U/L vs 120.2±8.7U/L) (P<0.05)。细胞凋亡明显加快(41.5%±2.3% vs 5.2%±0.5%，32.7%±1.8% vs 5.2%±0.5%)(P<0.05)， caspase-12、GRP78、CHOP、iNOS表达显著升高(P<0.05);与I/R组相比，I/R+ 1 400W组细胞活力升高，细胞培养液中LDH、凋亡水平、caspase-12、GRP78、CHOP下降(P<0.05)。结论1 400W可减轻人肝内胆管上皮细胞缺血再灌注损伤，其机制可能与抑制内质网应激有关。关键词:缺血再灌注损伤;胆管上皮细胞;一氧化氮合酶
{"title":"Inducible nitric oxide synthase inhibitor 1 400W suppresses endoplasmic reticulum stress and alleviates ischemia-reperfusion injury in human intrahepatic bile duct epithelial cells","authors":"Qiwen Yu, Hongwei Tang, Dongjing Yang, Wenzhi Guo, Jie Li","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.011","url":null,"abstract":"Objective \u0000To explore the role and mechanism of inducible nitric oxide synthase inhibitor 1 400W in alleviating ischemia-reperfusion injury of human intrahepatic bile duct epithelial cells. \u0000 \u0000 \u0000Methods \u0000Human intrahepatic bile duct epithelial cells (HIBEC) in logarithmic phase were inoculated into culture plate at an appropriate density. The samples were randomly divided into control group (group C), ischemia-reperfusion group (group I/R) and ischemia-reperfusion + 1 400W group (group I/R+ 1 400W). Group C was cultured routinely; cells in I/R and I/R+ 1 400W groups were placed in a three-gas incubator for 12h for simulating ischemia and then normal culture for 6h for simulating reperfusion. The I/R+ 1 400W group had a final concentration of 100 μmol/L of 1 400W before ischemia and hypoxia. After reperfusion, cells and culture medium were collected, CCK 8 was used for detecting cell vitality, microplate method for detecting the content of lactate dehydrogenase (LDH) in culture medium, AnnexinV-FITC/PI double stain for detecting apoptosis level, Western blot for analyzing the expressions of endoplasmic reticulum stress (ERS) related protein cysteinyl aspartic acid protease 12 (caspase-12), glucose regulatory protein 78 (GRP78) C/EBP homologous protein (CHOP) and inducible nitric oxide synthase (iNOS). \u0000 \u0000 \u0000Results \u0000As compared with group C, cell viability significantly decreased in I/R and I/R+ 1 400W groups (53.8%±2.3% vs.100%, 66.5%±2.8% vs.100%) (P<0.05) while LDH increased markedly in cell culture medium (287.4±9.0U/L vs 120.2±8.7U/L, 212.0±8.3U/L vs 120.2±8.7U/L) (P<0.05). Apoptosis accelerated markedly (41.5%±2.3% vs 5.2%±0.5%, 32.7%±1.8% vs 5.2%±0.5%) (P<0.05) and the expressions of caspase-12, GRP78, CHOP and iNOS spiked (P<0.05); as compared with I/R group, cell viability of I/R+ 1 400W group rose while LDH, apoptosis level, caspase-12, GRP78 and CHOP declined in cell culture medium (P<0.05). \u0000 \u0000 \u0000Conclusions \u00001 400W may alleviate ischemia-reperfusion injury of human intrahepatic bile duct epithelial cells and its mechanism may be correlated with a suppression of endoplasmic reticulum stress. \u0000 \u0000 \u0000Key words: \u0000Ischemia-reperfusion injury; Bile duct epithelial cells; Nitric oxide synthase","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"13 1","pages":"241-244"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87538469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.008
Longshan Liu, Jun Li, Q. Fu, Jiajia Jiang, Huanxi Zhang, R. Deng, Chenglin Wu, J. Fei, S. Deng, Guodong Chen, Gang Huang, J. Qiu, Lizhong Chen
Objective To assess the efficacy and safety of mizoribine (MZR) in initial immunosuppression in living-related renal transplant recipients. Methods From October 2015 to October 2017, twenty-two patients undergoing initial living-related renal transplantation received MZR (3-4 mg/kg/d) plus tacrolimus and corticosteroid. During a follow-up period of 12 months, patient/graft survival, incidence of acute rejection and adverse events were observed. Results There was no onset of graft loss and death and acute rejection rate was 22.7%. Renal allograft function remained stable. The incidence rate of cytomegaloviral infection was 4.5% and no CMV disease occurred. The incidence of BKV viruria was 36.4% and the infection rate was 18.2%. Digestive symptoms occurred (n=3, 13.6%). The major side effect of hyperuricemia could be controlled without reduction or withdrawal of MZR. Conclusions Excellent graft survival can be achieved when using MZR as initial immunosuppression in living-donor renal transplant recipients, yet the incidence of acute rejection remains high. Further study is required for determining the effect of MZR in the prevention of BK viral infection during renal transplantation. Key words: Kidney transplantation; Mizoribine; Acute rejection
{"title":"Efficacy and safety of initial-dose mizoribine plus tacrolimus and corticosteroids in living-related renal transplant recipients","authors":"Longshan Liu, Jun Li, Q. Fu, Jiajia Jiang, Huanxi Zhang, R. Deng, Chenglin Wu, J. Fei, S. Deng, Guodong Chen, Gang Huang, J. Qiu, Lizhong Chen","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.008","url":null,"abstract":"Objective \u0000To assess the efficacy and safety of mizoribine (MZR) in initial immunosuppression in living-related renal transplant recipients. \u0000 \u0000 \u0000Methods \u0000From October 2015 to October 2017, twenty-two patients undergoing initial living-related renal transplantation received MZR (3-4 mg/kg/d) plus tacrolimus and corticosteroid. During a follow-up period of 12 months, patient/graft survival, incidence of acute rejection and adverse events were observed. \u0000 \u0000 \u0000Results \u0000There was no onset of graft loss and death and acute rejection rate was 22.7%. Renal allograft function remained stable. The incidence rate of cytomegaloviral infection was 4.5% and no CMV disease occurred. The incidence of BKV viruria was 36.4% and the infection rate was 18.2%. Digestive symptoms occurred (n=3, 13.6%). The major side effect of hyperuricemia could be controlled without reduction or withdrawal of MZR. \u0000 \u0000 \u0000Conclusions \u0000Excellent graft survival can be achieved when using MZR as initial immunosuppression in living-donor renal transplant recipients, yet the incidence of acute rejection remains high. Further study is required for determining the effect of MZR in the prevention of BK viral infection during renal transplantation. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Mizoribine; Acute rejection","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"93 1","pages":"226-230"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86974540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.002
Yan Liu, Aomei Zhao, Xueni Lu, Qi Wang, Lulu Yang, Yuemin Zhang, Aimin Yang, P. Tian, Jianjun Xue
Objective To evaluate the significance of kidney depth obtained by computed tomography (CT) in measuring glomerular filtration rate (GFR) by Gates method in living kidney transplant donors. Methods Individual kidney depth was compared among the estimates of Tonnesen, Taylor and Li Qian formulas and CT measurements in 167 living-related kidney transplant donors respectively. While maintaining the active region of interest of kidney and background unchanged in 137 99mTc-DTPA renal dynamic imaging cases, GFR was measured by Gates' method and individual kidney compared among the estimates of Tonnesen, Taylor and Li Qian formulas and CT measurements. Results Left/right kidney depth obtained by CT, Tonnesen, Taylor and Li Qian formula was 6.82±0.96/7.02±1.00, 5.67±0.58/5.71±0.59, 6.43±0.77/6.81±0.72 and 7.03±0.76/7.06±0.70 cm; GFR 45.44±9.04/46.61±9.06, 37.54±6.34/37.37±6.02, 43.39±7.59/44.62±6.94 and 46.99±8.04/46.70±7.30 ml/min respectively. Individual kidney depth and GFR calculated by Taylor and Li Qian were higher than those of Tonnesen formula (P 0.05). Conclusions Kidney depth measured by CT improves the accuracy of kidney depth estimated by Gates method and optimizes GFR in living donors for renal transplant. Key words: Kidney transplantation; Glomerular filtration rate; Kidney depth
{"title":"Renal depth measured by CT optimize the glomerular filtration rate using the Gates method in living donor kidney transplantation","authors":"Yan Liu, Aomei Zhao, Xueni Lu, Qi Wang, Lulu Yang, Yuemin Zhang, Aimin Yang, P. Tian, Jianjun Xue","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.002","url":null,"abstract":"Objective \u0000To evaluate the significance of kidney depth obtained by computed tomography (CT) in measuring glomerular filtration rate (GFR) by Gates method in living kidney transplant donors. \u0000 \u0000 \u0000Methods \u0000Individual kidney depth was compared among the estimates of Tonnesen, Taylor and Li Qian formulas and CT measurements in 167 living-related kidney transplant donors respectively. While maintaining the active region of interest of kidney and background unchanged in 137 99mTc-DTPA renal dynamic imaging cases, GFR was measured by Gates' method and individual kidney compared among the estimates of Tonnesen, Taylor and Li Qian formulas and CT measurements. \u0000 \u0000 \u0000Results \u0000Left/right kidney depth obtained by CT, Tonnesen, Taylor and Li Qian formula was 6.82±0.96/7.02±1.00, 5.67±0.58/5.71±0.59, 6.43±0.77/6.81±0.72 and 7.03±0.76/7.06±0.70 cm; GFR 45.44±9.04/46.61±9.06, 37.54±6.34/37.37±6.02, 43.39±7.59/44.62±6.94 and 46.99±8.04/46.70±7.30 ml/min respectively. Individual kidney depth and GFR calculated by Taylor and Li Qian were higher than those of Tonnesen formula (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000Kidney depth measured by CT improves the accuracy of kidney depth estimated by Gates method and optimizes GFR in living donors for renal transplant. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Glomerular filtration rate; Kidney depth","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"6 1","pages":"195-199"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72872869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.003
Xihong Ge, Hang Li, Yan Sun, Mingyue Wang, Guangfeng Gao, Miaomiao Long, Xiaobin Liu, Jing Yu, XiaoMing Gong, Jingjing Tao, Zhiyan Lu, W. Shen
Objective To summarize the computed tomographic (CT) manifestations of pulmonary aspergillosis after organ transplantation and compare different signs between pulmonary aspergillosis and bacterial pneumonia. Methods CT images of pulmonary aspergillosis (n=62) and bacterial pneumonia (n=68) in post-transplantation patients were reviewed. The signs were categorized with consolidation, mass, large nodule (≥1cm), small nodule and bud-in-tree pattern. Some detailed useful differentiating signs such as halo sign, air bronchogram sign, reversed halo sign, hypodensity sign and cavitation were also analyzed. Results CT patterns of pulmonary aspergillosis included consolidation, mass, large nodule, small nodule and bud-in-tree pattern. The most common was large nodule (75.8%), followed by consolidation (48.4%) and mass (29.0%). And small nodule (16.1%) and bud-in-tree (12.9%) patterns were concurrent. For consolidation pattern, the proportion of bacterial pneumonia (69.1%) was the larger; For mass pattern, the proportion of pulmonary aspergillosis (29.0%) was the larger. For large nodule pattern, there was no difference. The detail sign of large nodule in two groups had no difference. In detailed signs of consolidation pattern, air bronchogram sign was more often seen in bacterial pneumonia while cavitation was more frequently found in pulmonary aspergillosis. In detailed signs of mass pattern, pulmonary aspergillosis often has single lesion (66.7%), cavitation (83.3%)and air crescent sign (77.8%) is more common. The proportion of halo sign was 30.7%. Conclusions CT manifestations of pulmonary aspergillosis are diverse after organ transplantation. There is some difference and yet overlap with bacterial pneumonia. Key words: Organ transplantation; Pulmonary aspergillosis; Computer tomography
{"title":"Computed tomographic manifestations of pulmonary aspergillosis after organ transplantation and differential diagnosis with bacterial infection","authors":"Xihong Ge, Hang Li, Yan Sun, Mingyue Wang, Guangfeng Gao, Miaomiao Long, Xiaobin Liu, Jing Yu, XiaoMing Gong, Jingjing Tao, Zhiyan Lu, W. Shen","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.003","url":null,"abstract":"Objective \u0000To summarize the computed tomographic (CT) manifestations of pulmonary aspergillosis after organ transplantation and compare different signs between pulmonary aspergillosis and bacterial pneumonia. \u0000 \u0000 \u0000Methods \u0000CT images of pulmonary aspergillosis (n=62) and bacterial pneumonia (n=68) in post-transplantation patients were reviewed. The signs were categorized with consolidation, mass, large nodule (≥1cm), small nodule and bud-in-tree pattern. Some detailed useful differentiating signs such as halo sign, air bronchogram sign, reversed halo sign, hypodensity sign and cavitation were also analyzed. \u0000 \u0000 \u0000Results \u0000CT patterns of pulmonary aspergillosis included consolidation, mass, large nodule, small nodule and bud-in-tree pattern. The most common was large nodule (75.8%), followed by consolidation (48.4%) and mass (29.0%). And small nodule (16.1%) and bud-in-tree (12.9%) patterns were concurrent. For consolidation pattern, the proportion of bacterial pneumonia (69.1%) was the larger; For mass pattern, the proportion of pulmonary aspergillosis (29.0%) was the larger. For large nodule pattern, there was no difference. The detail sign of large nodule in two groups had no difference. In detailed signs of consolidation pattern, air bronchogram sign was more often seen in bacterial pneumonia while cavitation was more frequently found in pulmonary aspergillosis. In detailed signs of mass pattern, pulmonary aspergillosis often has single lesion (66.7%), cavitation (83.3%)and air crescent sign (77.8%) is more common. The proportion of halo sign was 30.7%. \u0000 \u0000 \u0000Conclusions \u0000CT manifestations of pulmonary aspergillosis are diverse after organ transplantation. There is some difference and yet overlap with bacterial pneumonia. \u0000 \u0000 \u0000Key words: \u0000Organ transplantation; Pulmonary aspergillosis; Computer tomography","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"89 1","pages":"200-204"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85649896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.009
Qiuxiang Xia, Miaoyang Wang, Heng Li, Jing Liu, Chen Guo, Bo Li, Hui Zhang, Fei Xie, L. Fan, Hong-yan Zhu, Hua Su
Objective To explore the correlation between pre-transplantation donor kidney biopsy and short-term renal function after transplantation. Methods This study include 240 kidney transplantation of donation after cardiac death (DCD) from July 2016 to April 2018. Banff's score of donor kidney biopsy was employed for estimating kidney status. Results No significant correlation existed between rate of glomerulosclerosis and delayed graft function (DGF) (P=0.815). The rate of glomerulosclerosis was significantly correlated with 1-week estimated glomerular filtration rate (eGFR) and discharge eGFR (P 0.05). Arterial fibrosis was significantly positively correlated with DGF and negatively with 1-week eGFR and discharge eGFR (P<0.05). Statistically significant inter-group differences existed in 1-week eGFR and discharge eGFR that arterial fibrosis scores < 2 (n=19) and ≥2 (n=41) (P<0.05). Arteriolar hyalinosis score was negatively correlated with 1-week eGFR and discharge eGFR (P<0.05). Based upon arteriolar hyalinosis scores, they were divided into two groups < 2 (n=193) and ≥2 (n=47). There were significant inter-group differences in DGF, 1-week eGFR and discharge eGFR (P<0.05). Remuzzi scores were negatively correlated with 1-week eGFR and discharge eGFR (P<0.05). Interstitial fibrosis was significantly positively correlated with DGF (P<0.05) and negatively with 1-week eGFR and discharge eGFR (P<0.05). Conclusions Donor kidney glomerulosclerosis rate affects short-term renal function of recipients after transplantation. However, using 20% as a threshold value is limited in clinical practice. Arterial intimal fibrosis and arteriolar hyalinosis are important factors affecting short-term eGFR. Recipient kidneys with Remuzzi score >4 had poor renal function after transplantation. Interstitial fibrosis score may be used as a predictor of postoperative DGF and short-term renal function recovery. It is expected to be discussed more extensively in literature. Key words: Kidney transplantation; Renal needle biopsy; Delayed renal graft function
{"title":"Relationship between pre-transplantation kidney biopsy andshort-term renal function after transplantation","authors":"Qiuxiang Xia, Miaoyang Wang, Heng Li, Jing Liu, Chen Guo, Bo Li, Hui Zhang, Fei Xie, L. Fan, Hong-yan Zhu, Hua Su","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.009","url":null,"abstract":"Objective \u0000To explore the correlation between pre-transplantation donor kidney biopsy and short-term renal function after transplantation. \u0000 \u0000 \u0000Methods \u0000This study include 240 kidney transplantation of donation after cardiac death (DCD) from July 2016 to April 2018. Banff's score of donor kidney biopsy was employed for estimating kidney status. \u0000 \u0000 \u0000Results \u0000No significant correlation existed between rate of glomerulosclerosis and delayed graft function (DGF) (P=0.815). The rate of glomerulosclerosis was significantly correlated with 1-week estimated glomerular filtration rate (eGFR) and discharge eGFR (P 0.05). Arterial fibrosis was significantly positively correlated with DGF and negatively with 1-week eGFR and discharge eGFR (P<0.05). Statistically significant inter-group differences existed in 1-week eGFR and discharge eGFR that arterial fibrosis scores < 2 (n=19) and ≥2 (n=41) (P<0.05). Arteriolar hyalinosis score was negatively correlated with 1-week eGFR and discharge eGFR (P<0.05). Based upon arteriolar hyalinosis scores, they were divided into two groups < 2 (n=193) and ≥2 (n=47). There were significant inter-group differences in DGF, 1-week eGFR and discharge eGFR (P<0.05). Remuzzi scores were negatively correlated with 1-week eGFR and discharge eGFR (P<0.05). Interstitial fibrosis was significantly positively correlated with DGF (P<0.05) and negatively with 1-week eGFR and discharge eGFR (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Donor kidney glomerulosclerosis rate affects short-term renal function of recipients after transplantation. However, using 20% as a threshold value is limited in clinical practice. Arterial intimal fibrosis and arteriolar hyalinosis are important factors affecting short-term eGFR. Recipient kidneys with Remuzzi score >4 had poor renal function after transplantation. Interstitial fibrosis score may be used as a predictor of postoperative DGF and short-term renal function recovery. It is expected to be discussed more extensively in literature. \u0000 \u0000 \u0000Key words: \u0000Kidney transplantation; Renal needle biopsy; Delayed renal graft function","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"1995 1","pages":"231-236"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82444302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.004
Aini Abudusalamu, Y. Shao, A. Tuerganaili, B. Ran, T. Jiang, Shalayiadang Paizula, Ruiqing Zhang, Q. Guo, Aimaiti Dilimureti
Objective To evaluate the application value of preoperative computed tomography (CT) and three-dimensional reconstruction (3DR) of major vessels regarding lesion infiltration severity, vascular morphology & function and predict vascular surgeries during ex vivo liver resection and autotransplantation (ELRA) for end-stage hepatic alveolar echinococcosis (AE). Methods Preoperative radiological & angiographical, intraoperative photographic and pathological materials were retrospectively collected in 40 consecutive patients undergoing ELRA for end-stage hepatic alveolar echinococcosis from January 2017 to January 2019. Severity of lesion infiltration, morphological & functional status as well as predictive value for surgical planning of hepatic venous system (HVs), inferior vena cava (IVC), portal venous system (PVs) and hepatic arterial supply system (HAs) were estimated, then compared with those built golden standards: angiography, intraoperative diagnosis and pathological examinationss. Results Evaluation accuracy of CT and 3DR for lesion infiltration severity, vascular morphology & function and predicting vascular surgeries respectively were 68.1%, 71.9%, 78.9% and 57.5%, 89.2%, 71.1% and all paired data had statistical significance (all P<0.05). Furthermore, CT had a higher certainty of predicting the use of vascular prosthesis during surgery than 3DR (77.5% and 70.0%, P<0.05). Conclusions Pre-ELRA CT is recommended for evaluating lesion infiltration severity and predicting corresponding vascular surgery. However, 3DR has more certainty in evaluating vascular morphology & function. Furthermore, CT is more reliable than 3DR in predicting vascular prosthesis during ELRA. However, optional better solutions should be studied for higher assurance. Key words: Liver transplantation; Echinococcosis; Three-dimensional computer-aided design
{"title":"Value of preoperative computed tomography and three-dimensional reconstruction of major vascular structures before ex vivo liver resection and autotransplantation for hepatic alveolar echinococcosis","authors":"Aini Abudusalamu, Y. Shao, A. Tuerganaili, B. Ran, T. Jiang, Shalayiadang Paizula, Ruiqing Zhang, Q. Guo, Aimaiti Dilimureti","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.004","url":null,"abstract":"Objective \u0000To evaluate the application value of preoperative computed tomography (CT) and three-dimensional reconstruction (3DR) of major vessels regarding lesion infiltration severity, vascular morphology & function and predict vascular surgeries during ex vivo liver resection and autotransplantation (ELRA) for end-stage hepatic alveolar echinococcosis (AE). \u0000 \u0000 \u0000Methods \u0000Preoperative radiological & angiographical, intraoperative photographic and pathological materials were retrospectively collected in 40 consecutive patients undergoing ELRA for end-stage hepatic alveolar echinococcosis from January 2017 to January 2019. Severity of lesion infiltration, morphological & functional status as well as predictive value for surgical planning of hepatic venous system (HVs), inferior vena cava (IVC), portal venous system (PVs) and hepatic arterial supply system (HAs) were estimated, then compared with those built golden standards: angiography, intraoperative diagnosis and pathological examinationss. \u0000 \u0000 \u0000Results \u0000Evaluation accuracy of CT and 3DR for lesion infiltration severity, vascular morphology & function and predicting vascular surgeries respectively were 68.1%, 71.9%, 78.9% and 57.5%, 89.2%, 71.1% and all paired data had statistical significance (all P<0.05). Furthermore, CT had a higher certainty of predicting the use of vascular prosthesis during surgery than 3DR (77.5% and 70.0%, P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Pre-ELRA CT is recommended for evaluating lesion infiltration severity and predicting corresponding vascular surgery. However, 3DR has more certainty in evaluating vascular morphology & function. Furthermore, CT is more reliable than 3DR in predicting vascular prosthesis during ELRA. However, optional better solutions should be studied for higher assurance. \u0000 \u0000 \u0000Key words: \u0000Liver transplantation; Echinococcosis; Three-dimensional computer-aided design","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"329 1","pages":"205-210"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76804628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-20DOI: 10.3760/CMA.J.ISSN.0254-1785.2019.04.007
Tian Qin, Xiangqian Gu, J. Zheng, Jinchuan Liu, Yuanjia Tang, F. Xue
Objective To employ high-throughput next generation sequencing (NGS) for analyzing the expression of lncRNAs and mRNAs in donor samples from pediatric living donor liver transplantation and search differentially expressed lncRNAs and drug metabolic gene for individualized guidance of immunosuppressive agents. Methods Between October 2016 and December 2017, 10 liver tissue specimens from living donor liver transplantation children were collected and divided into fast and slow metabolic groups (n=5 each) according to the postoperative profiles of drug metabolism. Samples were assayed for high-throughput NGS. Target analysis was used for functional pathways and screening target genes prediction. Results There were differentially expressed 908 mRNAs and 1228 lncRNAs between slow metabolic and fast metabolic groups (P<0.05). According to the abundance and difference, 22 up-regulated and 18 down-regulated mRNAs, 13 up-regulated and 24 down-regulated lncRNAs were selected. In addition to CYP3A5, CYP2C19, CYP1A2 and UGT1A1 might affect the metabolism of tacrolimus. At the same time, NONHSAT108617.2 in differentially expressed lncRNAs might regulate the expression of CYP3A5 gene. Conclusions This study has comprehensively analyzed the expression of lncRNAs in donor liver from pediatric liver transplantation. Some differentially expressed drug metabolism related genes may affect tacrolimus metabolism in vivo and thus the postoperative use of immunosuppressive drugs. Key words: Liver transplantation; Long noncoding RNA; Drug metabolism
{"title":"Next generation sequencing analysis of drug metabolism related lncRNA in children after living donor liver transplantation","authors":"Tian Qin, Xiangqian Gu, J. Zheng, Jinchuan Liu, Yuanjia Tang, F. Xue","doi":"10.3760/CMA.J.ISSN.0254-1785.2019.04.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1785.2019.04.007","url":null,"abstract":"Objective \u0000To employ high-throughput next generation sequencing (NGS) for analyzing the expression of lncRNAs and mRNAs in donor samples from pediatric living donor liver transplantation and search differentially expressed lncRNAs and drug metabolic gene for individualized guidance of immunosuppressive agents. \u0000 \u0000 \u0000Methods \u0000Between October 2016 and December 2017, 10 liver tissue specimens from living donor liver transplantation children were collected and divided into fast and slow metabolic groups (n=5 each) according to the postoperative profiles of drug metabolism. Samples were assayed for high-throughput NGS. Target analysis was used for functional pathways and screening target genes prediction. \u0000 \u0000 \u0000Results \u0000There were differentially expressed 908 mRNAs and 1228 lncRNAs between slow metabolic and fast metabolic groups (P<0.05). According to the abundance and difference, 22 up-regulated and 18 down-regulated mRNAs, 13 up-regulated and 24 down-regulated lncRNAs were selected. In addition to CYP3A5, CYP2C19, CYP1A2 and UGT1A1 might affect the metabolism of tacrolimus. At the same time, NONHSAT108617.2 in differentially expressed lncRNAs might regulate the expression of CYP3A5 gene. \u0000 \u0000 \u0000Conclusions \u0000This study has comprehensively analyzed the expression of lncRNAs in donor liver from pediatric liver transplantation. Some differentially expressed drug metabolism related genes may affect tacrolimus metabolism in vivo and thus the postoperative use of immunosuppressive drugs. \u0000 \u0000 \u0000Key words: \u0000Liver transplantation; Long noncoding RNA; Drug metabolism","PeriodicalId":9885,"journal":{"name":"Chineae Journal of Organ Transplantation","volume":"19 1","pages":"219-225"},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72956441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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