Chinese Herbal Medicines最新文献

{"title":"Therapeutic potentials of natural products for post-traumatic stress disorder: A focus on epigenetics","authors":"Meijing Xu,&nbsp;Minghui Cui,&nbsp;Yu Wang,&nbsp;Boru Li,&nbsp;Lijin Feng,&nbsp;Hang Xing,&nbsp;Kuo Zhang","doi":"10.1016/j.chmed.2024.07.004","DOIUrl":"10.1016/j.chmed.2024.07.004","url":null,"abstract":"<div><div>Post-traumatic stress disorder (PTSD) is a relatively common but complex mental illness with a range of diverse risk factors. Typical symptoms include the re-experience or avoidance of traumatic events, cognitive impairment, and hypervigilance. While the exact pathogenesis of PTSD is unclear, many studies indicate that epigenetic regulation plays a key role in its development. Specifically, numerous studies have indicated that the levels of histone acetylation and methylation, DNA methylation, and noncoding RNA are altered in PTSD patients. Further to this, natural products have been found to achieve epigenetic regulation of PTSD by regulating the expression of epigenetic enzymes, long noncoding RNA (lncRNA), and miRNA, thereby playing a role in improving PTSD symptoms. To date, however, no epigenetic regulation related drugs have been used in the treatment of PTSD. Furthermore, while natural products that can epigenetically regulate PTSD have received increasing levels of attention, there have not yet been any systematic reports on the topic. Here, we summarized the roles and mechanisms of natural products in the epigenetic regulation of PTSD, providing a novel and unique perspective that will help to guide the development and application of new PTSD treatments.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 2","pages":"Pages 203-219"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modernization of charcoal drugs: Integrating research paradigms of carbon dots to gain new perspectives","authors":"Sichao Tian ,&nbsp;Zhanglu Hu ,&nbsp;Weidong Zhang","doi":"10.1016/j.chmed.2025.01.004","DOIUrl":"10.1016/j.chmed.2025.01.004","url":null,"abstract":"<div><div>With the modernization drive of traditional Chinese medicine (TCM), this perspective innovatively proposes integrating carbon dot research paradigms to facilitate the modernization of charcoal drugs in TCM. The research focuses on five core areas: analyzing and validating charcoal drugs components pharmacologically, exploring modern preparation methods, establishing a quality evaluation system, fixing preparation process parameters, and probing into the material basis. These steps aim to deepen the scientific understanding of the material basis of charcoal drugs, optimize its preparation process, and establish a comprehensive quality control system. This work provides a theoretical foundation and experimental evidence for the scientific understanding of charcoal drugs, further promoting their modernization and internationalization.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 2","pages":"Pages 292-295"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorating vascular endothelial injury for lipolysacharide-induced via mitochondrial targeting function of octaarginine-modified essential oil from Fructus Alpiniae zerumbet (EOFAZ) lipid microspheres","authors":"Lingyan Li ,&nbsp;Zengqiu Yang ,&nbsp;Qiqi Li ,&nbsp;Qianqian Guo ,&nbsp;Xingjie Wu ,&nbsp;Yu’e Wang ,&nbsp;Xiangchun Shen ,&nbsp;Ying Chen ,&nbsp;Ling Tao","doi":"10.1016/j.chmed.2024.11.004","DOIUrl":"10.1016/j.chmed.2024.11.004","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the therapeutic potential of octaarginine (R8)-modified essential oil from Fructus <em>Alpiniae zerumbet</em> (EOFAZ) lipid microspheres (EOFAZ@^R8LM) for cardiovascular therapy.</div></div><div><h3>Methods</h3><div>EOFAZ@^R8LM was developed by leveraging the volatilization of EOFAZ and integrating it with the oil phase of LM, followed by surface modification with cell-penetrating peptide R8 to target the site of vascular endothelial injury. The therapeutic effects of this formulation in alleviating lipopolysaccharide-induced vascular endothelial inflammation were evaluated by assessing mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) levels, as well as inflammatory factors interleukin-6 (IL-6) and interleukin-1<em>β</em> (IL-1<em>β</em>) levels.</div></div><div><h3>Results</h3><div>EOFAZ@^R8LM effectively delivered EOFAZ to the site of injury and specifically targeted the mitochondria in vascular endothelial cells, thereby ameliorating mitochondrial dysfunction through regulation of MMP and reduction of intracellular ROS levels. Moreover, it attenuated the expression levels of IL-6 and IL-1<em>β</em>, exerting protective effects on the vascular endothelium.</div></div><div><h3>Conclusion</h3><div>Our findings highlight the significant therapeutic potential of EOFAZ@^R8LM in cardiovascular therapy, providing valuable insights for developing novel dosage forms utilizing EOFAZ for effective treatment against cardiovascular diseases.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 2","pages":"Pages 340-351"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Banxia Xiexin Decoction inhibits colitis-associated colorectal cancer development by modulating STAT3 signaling and gut microbiota","authors":"Yinzi Yue ,&nbsp;Lianlin Su ,&nbsp;Yahui Wang ,&nbsp;Xiaoman Li ,&nbsp;Xiaoyan Xiao ,&nbsp;Jin Xie ,&nbsp;Shuai Yan","doi":"10.1016/j.chmed.2024.02.004","DOIUrl":"10.1016/j.chmed.2024.02.004","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the therapeutic effects of Banxia Xiexin Decoction (BXD), a herbal medicine formula, on inflammation and the imbalance of the gut microbiota in a rat model of colorectal cancer (CRC) induced by azoxymethane (AOM) /dextran sulfate sodium (DSS).</div></div><div><h3>Methods</h3><div>A total of 75 male C57BL/6 mice were randomly divided into five groups: normal control group (NC), model group (MODEL), low-dose BXD treatment group (L-BXD), high-dose BXD treatment (H-BXD) group and MS treatment group (MS). BXD and MS were used in CRC mice at the doses of 3.915 g/kg, 15.66 g/kg, 0.6 g/kg for 3 weeks consecutively. Histopathological changes in the colon were observed using hematoxylin-eosin (HE) staining. The content of inflammatory factors in serum was detected by an enzyme-linked immunosorbent assay (ELISA), and the expression of mRNA and protein of genes related to immunity, apoptosis, inflammation, and inflammatory factors was evaluated. Changes in the intestinal flora of mouse fecal were determined based on high-throughput sequencing of the 16S rRNA microbial gene.</div></div><div><h3>Results</h3><div>Compared to the model group, the low-dose BXD and high-dose BXD groups decreased the number of colon tumors, reversed weight loss, and shortened colon length of mice. The pathological examination showed that BXD alleviated the malignancy of intestinal tumors. It also suppressed signal transducer and activator of transcription 3 (STAT3), matrix metalloproteinase-9 (MMP-9), and transforming growth factor beta 1 (TGF-β1) expression, while increasing the expression of the tight junction protein ZO-1 in colon tissues. Additionally, the levels of key pathway proteins involved in inflammation (phosphorylated-STAT3, Bcl-2, COX-2) and cell cycle regulatory molecules (c-Myc and PCNA) were reduced. According to 16S rRNA sequence analysis, BXD enhanced the relative abundance of potentially beneficial bacteria, while that of cancer-related bacteria decreased.</div></div><div><h3>Conclusion</h3><div>BXD plays a preventive role in developing colorectal cancer; its mechanisms are related to the inhibition of inflammation and tumor proliferation, as well as maintenance of intestinal homeostasis.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 2","pages":"Pages 380-391"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141135570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new polyketides from Rhodiola tibetica endophytic fungus Penicillium sp. HJT-A-6","authors":"Dongliang Xiao ,&nbsp;Xiaobao Li ,&nbsp;Xuemei Zhang ,&nbsp;Nan Jiang ,&nbsp;Dunzhu Luosang ,&nbsp;Weixing Feng ,&nbsp;Xuan Lu ,&nbsp;Baomin Feng","doi":"10.1016/j.chmed.2024.06.004","DOIUrl":"10.1016/j.chmed.2024.06.004","url":null,"abstract":"<div><h3>Objective</h3><div>To study bioactive compounds from the endophytic fungus <em>Penicillium</em> sp. HJT-A-6 isolated from stem of <em>Rhodiola tibetica</em>, and evaluate its allelopathic activity.</div></div><div><h3>Methods</h3><div>The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. In addition, the allelopathic activity of compound <strong>1</strong> was evaluated by measuring the seed germination rate of <em>R. tibetica</em>.</div></div><div><h3>Results</h3><div>Two new polyketides 4-hydroxy-3,6-dimethyl-2<em>H</em>-pyran-2-one (<strong>1</strong>) and penilactone E (<strong>2</strong>), together with six known compounds walterolactone A (<strong>3</strong>), 5-hydroxyhexan-4-olide (<strong>4</strong>), 3-methyl-2-penten-5-olide (<strong>5</strong>), chaetoquadrin F (<strong>6</strong>), (<em>Z</em>)-6-acetyl-3-(1,2-dihydroxypropylidene)-5-hydroxy-8-methylchroman-2-one (<strong>7</strong>) and 4-hydroxy-3-(4-hydroxyhexanoyl)-5-methylfuran-2(5<em>H</em>)-one (<strong>8</strong>) were isolated from <em>Penicillium</em> sp. HJT-A-6. Compound <strong>1</strong> showed moderate seed-germination-promoting activity at a concentration of 0.001 mg/mL while inhibiting the seed germination at concentrations of 0.1 and 0.01 mg/mL. Compared with the positive drug 6-benzyladenine (6-BA), compound <strong>1</strong> could extend the seed-germination period of <em>R. tibetica</em> (up to 11 d).</div></div><div><h3>Conclusion</h3><div>Two new compounds were isolated from <em>R. tibetica</em> endophytic fungus <em>Penicillium</em> sp. HJT-A-6. Compound <strong>1</strong> displayed plant hormone-like activity, which inhibited the seed germination of the host plant at high concentrations and promoted the seed germination of the host plant at low concentrations. The results not only enrich the chemical constituents of the endophytic fungi isolated from <em>Rhodiola tibetica</em>, but also provide a theoretical basis for understanding the interaction mechanism between <em>Rhodiola tibetica</em> endophytic fungi and the host plant.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 2","pages":"Pages 404-408"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavones in pomelo peel resist fibril formation of human islet amyloid polypeptide","authors":"Cuiyun Gao,&nbsp;Zhiruo Wan,&nbsp;Yan Liu,&nbsp;Yuting Meng,&nbsp;Xu Chen,&nbsp;Xiaohan Tang,&nbsp;Lingyu Hang,&nbsp;Hailong Yuan","doi":"10.1016/j.chmed.2024.02.002","DOIUrl":"10.1016/j.chmed.2024.02.002","url":null,"abstract":"<div><h3>Objective</h3><div>Exploring the formation and aggregation of human islet amyloid polypeptide (hIAPP) (amylin) fibers is significant for promoting the prevention and treatment of type II diabetes mellitus (T2DM). Flavones in pomelo peel have visible biological activity in the anti-diabetes aspect. The present study aimed to investigate the effects of five flavones [naringin (NRG), narirutin (NRR), nobiletin (NOB), sinensetin (SIN), and neohesperidin (NHP)] in pomelo peel on peptide aggregation and explore its possible mechanisms. The cell viability of flavones against peptide aggregation was also evaluated.</div></div><div><h3>Methods</h3><div>The thioflavin T (ThT) assay and transmission electron microscopy (TEM) were used for evaluating the inhibition and disaggregation of flavones on peptide aggregation. The interaction mechanism was analyzed by endogenous fluorescence, molecular dynamics (MD) simulations, ultraviolet spectroscopy (UV) and isothermal titration calorimetry (ITC) experiments. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and immune assays were performed to characterize the cell viability of flavones against peptide aggregation.</div></div><div><h3>Results</h3><div>The five flavones showed a decrease in fluorescence intensity, fiber number and size under incubation with different molar ratios of hIAPP. The compounds can bind to the aromatic tyrosine (Tyr) residueTyr 37, resulting in the intrinsic fluorescence quenching of the peptides. Five flavones can form hydrogen bonds with hIAPP, which is likely to be based on their phenolic hydroxyl structure. They showed strong binding affinity with peptides. The reaction system of NRG and NRR observed an exothermic reaction, and the others were endothermic reactions. The absorption peaks of the compounds with hIAPP changed and showed hypochromic effects, indicating that there may be π-π stacking interaction. Flavones noticeably increased the cell viability in the presence of amyloid peptides and reduced the absorption intensity induced by peptide oligomers.</div></div><div><h3>Conclusion</h3><div>A total of five flavones in pomelo peel have inhibitory and depolymerization effects on amyloid fibrils, and can significantly protect cells from the toxic effect of hIAPP and reduce the production of toxic oligomers.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 1","pages":"Pages 166-177"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sini decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism","authors":"Cuiping Long ,&nbsp;Qian Zhou ,&nbsp;Min Xu ,&nbsp;Xin Ding ,&nbsp;Xingxing Zhang ,&nbsp;Ya Zhang ,&nbsp;Yuping Tang ,&nbsp;Guangguo Tan","doi":"10.1016/j.chmed.2023.12.004","DOIUrl":"10.1016/j.chmed.2023.12.004","url":null,"abstract":"<div><h3>Objective</h3><div>Myocardial inflammation during myocardial infarction (MI) could be inhibited by regulating arachidonic acid (AA) metabolism. Recent studies demonstrated that Sini Decoction (SND) was identified to be an effective prescription for treating heart failure (HF) caused by MI. But the anti-inflammatory mechanism of SND remained unclear. The work was designed to investigate the anti-inflammatory mechanism of SND through the AA metabolism pathway <em>in vitro</em> and <em>in vivo</em> experiments.</div></div><div><h3>Methods</h3><div>An inflammatory injury model of H9c2 cells was established by lipopolysaccharide (LPS)-stimulated macrophage-conditioned medium (CM). The MI model was built by the ligation of left anterior descending (LAD) branch of coronary artery in rat. Meanwhile, the rats were divided into five groups: sham group, MI group, MI + Celecoxib group, MI + low-dose SND group (SND-L) and MI + high-dose SND group (SND-H). Cardiac function, histopathological changes and serum cytokines were examined four weeks later. Western blot analysis was conducted to verify the key enzymes levels in the AA metabolic pathway, including phospholipase A2 (PLA2), cyclooxygenases (COXs) and lipoxygenases (LOXs).</div></div><div><h3>Results</h3><div>These <em>in vivo</em> results demonstrated that SND could improve the cardiac function and pathological changes of rats with MI, and regulate the key inflammatory molecules in the AA metabolism pathway, including sPLA2, COX-1, COX-2, 5-LOX and 15-LOX. <em>In vitro</em>, SND could decrease the release of pro-inflammatory cytokines including TNF-α and IL-6 and inhibit cell apoptosis in CM-induced H9c2 cells. Moreover, SND could protect H9c2 cells from the damage of CM by regulating nuclear factor kappa-B (NF-κB) signal pathway and the expression of COX-2.</div></div><div><h3>Conclusion</h3><div>SND may be a drug candidate for anti-inflammatory treatment during MI by regulating the multiple targets in the AA metabolism pathway.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 1","pages":"Pages 148-155"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140404652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in phytochemistry, ananlysis methods and pharmacology of Eleutherococcus trifoliatus: A promising medicinal and edible resource with development value","authors":"Maofang Lu ,&nbsp;Bin Wang ,&nbsp;Ling Dai ,&nbsp;Jian Wu ,&nbsp;Jiao Luo ,&nbsp;Changsoo Yook ,&nbsp;Xiangqian Liu","doi":"10.1016/j.chmed.2024.10.001","DOIUrl":"10.1016/j.chmed.2024.10.001","url":null,"abstract":"<div><div><em>Eleutherococcus trifoliatus</em> (<em>Araliaceae</em>) is called Baile or Lecai in China. <em>E. trifoliatus</em> is a medicinal and edible plant widely used in folk traditions. As a TCM, the dried herb of this species can remove damp heat and detoxicity, cure rheumatism, remove blood stasis, relieve pain, and alleviate cough and asthma symptoms. Many chemical compounds have been reported including diterpenoids, triterpenoids, phenylpropanoids, flavonoids, lignans, caffeoyl quinic acids, steroids, essential oils, etc., in which flavonoids, saponins, and caffeoyl quinic acids are the most bioactive components. <em>In vitro</em> and <em>in vivo</em> pharmacological experiments demonstrated that <em>E. trifoliatus</em> has anti-inflammatory, hypoglycemic, anticancer, antioxidant, antibacterial, anti-hyperalgesic, anti-fatigue, analgesic, and hemostatic effects. Here we reviewed <em>E. trifoliatus</em> in phytochemistry, analysis methods, and pharmacology.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 1","pages":"Pages 19-30"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paris saponin VII induces Caspase-3/GSDME-dependent pyroptosis in pancreatic ductal adenocarcinoma cells by activating ROS/Bax signaling","authors":"Xiaoying Qian ,&nbsp;Yang Liu ,&nbsp;Wenwen Chen ,&nbsp;Shuxian Zheng ,&nbsp;Yunyang Lu ,&nbsp;Pengcheng Qiu ,&nbsp;Xisong Ke ,&nbsp;Haifeng Tang ,&nbsp;Xue Zhang","doi":"10.1016/j.chmed.2024.04.004","DOIUrl":"10.1016/j.chmed.2024.04.004","url":null,"abstract":"<div><h3>Objective</h3><div><em>Paridis Rhizoma</em> (Chonglou in Chinese), a traditional Chinese herbal medicine, has been shown have strong anti-tumor effects. Paris saponin VII (PSVII), an active constituent isolated from <em>Paridis Rhizoma</em>, was demonstrated to significantly suppress the proliferation of BxPC-3 cells in our previous study. Here, we aimed to elucidate the anti-pancreatic ductal adenocarcinoma (PDAC) effect of PSVII and the underlying mechanism.</div></div><div><h3>Methods</h3><div>Cell viability was determined by CCK-8, colony formation, and cell migration assays. Cell apoptosis and reactive oxygen species (ROS) production were measured by flow cytometry with annexin V/propidine iodide (Annexin V/PI) and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), respectively. Pyroptosis was evaluated by morphological features, Hoechst 33342/PI staining assay, and release of lactate dehydrogenase (LDH). JC-1 fluorescent dye was employed to measure mitochondrial membrane potential. Western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to determine the levels of proteins or mRNAs. The effect <em>in vivo</em> was assessed by a xenograft tumor model.</div></div><div><h3>Results</h3><div>PSVII inhibited the viability of PDAC cells (BxPC-3, PANC-1, and Capan-2 cells) and induced gasdermin E (GSDME) cleavage, as well as the simultaneous cleavage of Caspase-3 and poly (ADP-ribose) polymerase 1 (PARP). Knockdown of GSDME shifted PSVII-induced pyroptosis to apoptosis. Additionally, the effect of PSVII was significantly attenuated by Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone (Z-DEVD-FMK), on the induction of GSDME-dependent pyroptosis. PSVII also elevated intracellular ROS accumulation and stimulated Bax and Caspase-3/GSDME to conduct pyroptosis in PDAC cells. The ROS scavenger <em>N</em>-acetyl cysteine (NAC) suppressed the release of LDH and inhibited Caspase-9, Caspase-3, and GSDME cleavage in PDAC cells, ultimately reversing PSVII-induced pyroptosis. Furthermore, in a xenograft tumor model, PSVII markedly suppressed the growth of PDAC tumors and induced pyroptosis.</div></div><div><h3>Conclusion</h3><div>These results demonstrated that PSVII exerts therapeutic effects through Caspase-3/GSDME-dependent pyroptosis and may constitute a novel strategy for preventing chemotherapeutic resistance in patients with PDAC in the future.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 1","pages":"Pages 94-107"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141145254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploitation potential and deep-rooted conformational relationships of traditional Chinese medicine polysaccharides","authors":"Wenyuan Gao","doi":"10.1016/j.chmed.2024.12.005","DOIUrl":"10.1016/j.chmed.2024.12.005","url":null,"abstract":"","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 1","pages":"Pages 1-2"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}