Chinese Herbal Medicines最新文献

{"title":"Unveiling secrets of traditional Chinese medicine: Cutting-edge techniques in component analysis","authors":"Tingting Zhou","doi":"10.1016/j.chmed.2025.05.006","DOIUrl":"10.1016/j.chmed.2025.05.006","url":null,"abstract":"<div><div>Chemical component analysis is a critical challenge in Chinese herbal medicine research, involving the qualitative and quantitative identification of complex constituents in traditional Chinese medicine (TCM). However, traditional analytical methods are insufficient for efficient and comprehensive analysis of complex composition of TCM. Limitations exist in sample preparation, instrumental technology, data processing, and activity-related quality marker research. Recent advancements have significantly improved analytical precision, enabling more comprehensive profiling of TCM components. New pretreatment methods improve extraction efficiency and detection sensitivity, while novel instrumental technologies, such as mass spectrometry imaging, preserve spatial information lost in homogenization. AI enhances data interpretation, improving accuracy and efficiency. Online activity analysis links chemical composition with bioactivity, overcoming the limitations of purely chemical profiling and enabling a more comprehensive evaluation of TCM efficacy. This perspective provides an overview of the development trends in component analysis, aiming to advance the field and support TCM modernization.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 484-487"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shufeng Jiedu Granule against mild COVID-19: Protocol of the randomized, double-blind, placebo-controlled, multi-center heal-COVID phase III study","authors":"Li Yang ,&nbsp;Thomas Friedemann ,&nbsp;Jun Pan ,&nbsp;Xiangyu Li ,&nbsp;Fuxiang Wang ,&nbsp;Yuanlong Lin ,&nbsp;Qiang Zhu ,&nbsp;Sven Schröder ,&nbsp;Qingsong Liu ,&nbsp;Hongzhou Lu","doi":"10.1016/j.chmed.2025.04.005","DOIUrl":"10.1016/j.chmed.2025.04.005","url":null,"abstract":"<div><h3>Objective</h3><div>Since Omicron will likely persist, this trial evaluates the safety and efficacy of Shufeng Jiedu Granule (SFJDG) for mild Omicron infection, aims at finding new therapies especially for home-treated patients.</div></div><div><h3>Methods</h3><div>This randomized, double-blind, placebo-controlled, multi-center phase III trial involves 844 patients, divided into a treatment group (422) and control group (422). Participants will receive SFJDG or placebo for 7 d (1.2 g/bag, 2 bags, 3 times/d). Hospital evaluations will be done on days 1 and 8, with telephone assessments on days 3 and 5. Follow-up continues on days 10 and 14. Diary cards will track symptom scores and safety data. The primary outcome is the time to sustained clinical recovery from corona virus disease 2019 (COVID-19) symptoms. An interim analysis will occur after 70 % of patients complete follow-up, with Type I error correction (<em>α</em>1 = 0.015) at interim analysis based on O’Brien-Fleming-type cumulative error spending function.</div></div><div><h3>Results</h3><div>This phase III trial evaluates the efficacy and safety of SFJDG for mild COVID-19, focusing on real-world applicability for home-managed patients. The study’s randomized, double-blind, placebo-controlled design ensures methodological rigor, while its comprehensive outcome measures address both symptom recovery and treatment safety. By emphasizing symptom resolution and recovery time, the trial aligns with the clinical priorities for managing mild cases of COVID-19. The findings could offer valuable insights into SFJDG’s role in improving patient outcomes and addressing gaps left by existing antiviral therapies, particularly in symptom management.</div></div><div><h3>Conclusion</h3><div>The global risk assessment remains high due to the ongoing virulence of SARS-CoV-2 Omicron sub-lineages. This Phase III study adopts a robust methodology to investigate SFJDG as a treatment for mild COVID-19 as well as it’s effectiveness and safety. Furthermore, this study aim to provide sufficient scientific evidence for the market registration of SFJDG especially for home-treated patients. If successful, SFJDG could be a meaningful addition to therapeutic options for mild infections, supporting public health strategies in managing the ongoing impact of SARS-CoV-2.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 601-608"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine formulas alleviated acute pancreatitis via improvement of microcirculation: A systematic review and meta-analysis","authors":"Ji Gao ,&nbsp;Chenxia Han ,&nbsp;Ning Dai ,&nbsp;Wen Wang ,&nbsp;Tao Jin ,&nbsp;Dan Du ,&nbsp;Qing Xia","doi":"10.1016/j.chmed.2024.12.002","DOIUrl":"10.1016/j.chmed.2024.12.002","url":null,"abstract":"<div><h3>Objective</h3><div>Microcirculatory disturbance is pathologically critical to acute pancreatitis (AP), which can be effectively alleviated by traditional Chinese medicine (TCM) formulas that activate blood flow. However, there has been no evidence-based research to date. Therefore, a well-designed systematic review and meta-analysis is necessary to elucidate the therapeutic transformative benefit of improving microcirculation during AP. This study aims to confirm the therapeutic efficacy of TCM formulas and explore the potential mechanisms underlying their effects on AP treatment.</div></div><div><h3>Methods</h3><div>Studies from eight databases including Pubmed, Embase, Web of Science, Cochrane Library, CNKI, CBM, Wanfang, and Chinese VIP, were screened for the eligible randomized controlled trials (RCTs). The APACHE II score and effectiveness rate were set as primary outcomes, while mortality rate, complications, total hospital stays, serum amylase recovery time, the time until the disappearance of abdominal pain, microcirculation indicators, and inflammation indicators were chosen as secondary outcomes. A systematic review and meta-analysis were subsequently conducted. Network pharmacology analysis was performed to analyze potential bioactive components with relevant targets of the core herbs included in the TCM formulas for activating blood flow.</div></div><div><h3>Results</h3><div>A total of 51 RCTs (<em>n</em> = 3 721) were included. Compared with conventional western medical treatments alone, TCM groups were associated with lower APACHE II score (SMD =  − 1.36, 95% CI: −2.01 to − 0.71, <em>P</em> = 0.000) and higher effectiveness rate (RR: 1.22, 95% CI: 1.18 to 1.26, <em>P</em> = 0.000). Furthermore, the formulas for activating blood flow demonstrated significant efficacy in improving both microcirculation and inflammation indicators. Additionally, six core Chinese herbal medicines including <em>Rhei Radix</em> et <em>Rhizoma</em> with the highest frequency, <em>Aurantii Fructus Immaturus</em>, <em>Paeoniae Radix Rubra</em>, <em>Bupleuri Radix</em>, <em>Salviae Miltiorrhizae Radix</em> et <em>Rhizoma</em>, and <em>Corydalis Rhizoma</em> were filtered out from the adopted TCM formulas. Finally, 166 shared targets between the six herbs and AP were identified. KEGG analysis indicated that lipid and atherosclerosis pathway is highly related to microcirculation.</div></div><div><h3>Conclusion</h3><div>TCM formulas for activating blood flow significantly improve microcirculation and alleviate AP. Further high-quality, well-designed RCTs and deep mechanism exploration are required.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 584-600"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress of regulating intestinal flora by traditional Chinese medicine in treating coronary heart disease","authors":"Qiwen Lu ,&nbsp;Sang Luo ,&nbsp;Chengyan Guan ,&nbsp;Hao Zhang ,&nbsp;Haoyue Jia ,&nbsp;Qiang Wan","doi":"10.1016/j.chmed.2025.04.007","DOIUrl":"10.1016/j.chmed.2025.04.007","url":null,"abstract":"<div><div>Coronary atherosclerotic heart disease is a major cardiovascular condition driven by atherosclerosis, distinguished by chronic inflammation and dysregulated lipid metabolism. The gut microbiota plays an essential role in human health and disease, with research indicating a strong association between gut microbial metabolism and the development and progression of coronary heart disease. This article provides a review of the relationship between gut microbiota and coronary heart disease, as well as the mechanisms by which traditional Chinese medicine regulates digestive tract microbiota to treat coronary heart disease, which systematically explains how the gut microbiota, through metabolic products and immune regulation, contributes to the occurrence and progression of coronary heart disease, and summarizes recent advances in research on traditional Chinese medicine’s regulation of gut microbiota for treating coronary heart disease. It aims to provide further reference and insights for exploring the relationship between gut microbiota and coronary heart disease, as well as traditional Chinese medicine approaches for treating coronary heart disease.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 464-472"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on comprehensive quality consistency evaluation strategy for TCM Granules: A case study with sugar-free Yangwei Granules produced by fluid-bed granulation","authors":"Jie Zhao,&nbsp;Geng Tian,&nbsp;Haibin Qu","doi":"10.1016/j.chmed.2024.11.001","DOIUrl":"10.1016/j.chmed.2024.11.001","url":null,"abstract":"<div><h3>Objective</h3><div>To develop a quality consistency evaluation strategy for traditional Chinese medicine (TCM) granules using sugar free Yangwei Granules as a model drug, and demonstrate the effectiveness of the developed method.</div></div><div><h3>Methods</h3><div>The strategy integrates several methods including, HPLC fingerprint and physical fingerprint methods analyze the similarity in chemical and physical properties of the TCM granule samples. Near-infrared (NIR) spectroscopy with principal components cluster analysis method is used to monitor normal operating conditions (NOC) samples accurately and to identify different types of abnormal operating conditions (AOC) samples, particularly those that deviate from the normal range.</div></div><div><h3>Results</h3><div>The combined use of HPLC fingerprint and physical fingerprint provides insights into the chemical and physical properties of the samples. NIR spectroscopy, combined with principal components cluster analysis, achieves high accuracy in monitoring NOC samples and identifying AOC samples without misjudgment. The approach proves useful as a complementary method in cases where HPLC fingerprint and physical fingerprint alone lack sufficient resolution.</div></div><div><h3>Conclusion</h3><div>This study establishes the feasibility and utility of the integrated approach for assessing the quality consistency of TCM granules. The strategy shows a high degree of generalization and holds significant importance for enhancing the quality control processes of TCM granules.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 575-583"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyssopus cuspidatus extract inhibited OVA-sensitized allergic asthma through PI3K/JNK/P38 signaling pathway and lipid homeostasis regulation","authors":"Yali Zhang ,&nbsp;Huiming Peng ,&nbsp;Jingjing Li ,&nbsp;Pan Lv ,&nbsp;Mengru Zhang ,&nbsp;Xu Wang ,&nbsp;Siyu Wang ,&nbsp;Siying Zhu ,&nbsp;Jiankang Lu ,&nbsp;Xuepeng Fan ,&nbsp;Jinbo Fang","doi":"10.1016/j.chmed.2024.11.009","DOIUrl":"10.1016/j.chmed.2024.11.009","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect and mechanism of <em>Hyssopus cuspidatus</em> Boriss. extract (HCE) in ovalbumin (OVA)-induced allergic asthma.</div></div><div><h3>Methods</h3><div>Components identification of HCE was conducted using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry. Mice were sensitized with OVA to establish asthmatic model, and dexamethasone was used as positive control. Respiratory reactivity, white cells counting in bronchoalveolar lavage fluid and peripheral blood, cytokine level measurement in serum and lung tissue, and histologic examination were performed to evaluate the therapeutic effect of HCE on asthma. Network pharmacology approach was used for mechanism prediction. Western blotting and untargeted lipidomics method were applied for mechanism validation.</div></div><div><h3>Results</h3><div>Fifty-two compounds were identified in HCE, predominantly terpenoids and flavonoids. HCE markedly reduced airway resistance, the eosinophil infiltration in lung tissues, and the levels of immunoglobulin E, interleukin-4, interleukin-5, and interleukin-13. Network pharmacology analysis suggested phosphatidylinositol 3-kinases (PI3K), c-Jun <em>N</em>-terminal kinase (JNK), and p38 Mitogen-activated protein kinase (p38 MAPK) may be key proteins of HCE in the treatment of allergic asthma. Western blot results indicated that the levels of phosphorylated PI3K, JNK, and P38 were downregulated in HCE-treated group. Moreover, HCE significantly upregulated the levels of ceramide and sphingomyelin and downregulated the level of phosphatidylcholine.</div></div><div><h3>Conclusion</h3><div>HCE inhibited allergic asthma via PI3K/JNK/P38 signaling pathway and lipid homeostasis regulation.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 539-547"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthraquinones of Cassiae Semen alleviate lipid accumulation in obesity by regulating brown adipose tissue and liver function","authors":"Yijie Li ,&nbsp;Ruiyu Wu ,&nbsp;Xin Li ,&nbsp;Jianan Li ,&nbsp;Yinhao Zhang ,&nbsp;Yanbo Huang ,&nbsp;Guifang Fan ,&nbsp;Xiaojiaoyang Li","doi":"10.1016/j.chmed.2025.02.003","DOIUrl":"10.1016/j.chmed.2025.02.003","url":null,"abstract":"<div><h3>Objective</h3><div><em>Cassiae Semen</em> (CS, Juemingzi in Chinese) is a widely used traditional Chinese medicine with a variety of pharmacological effects. This study aimed to investigate the potential therapeutic effects and molecular mechanisms of anthraquinones of CS (AQS) for adiposity.</div></div><div><h3>Methods</h3><div>The chemical components of the AQS were determined using high-performance liquid chromatography (HPLC). Network pharmacology analysis was used to predict potential anti-obesity targets of action for AQS. We constructed high fat with high sugar water diet-induced obese mice and observed their body weight and whole-body lipid metabolism to evaluate the efficacy of AQS in promoting lipid metabolism. Subsequently, the epidermal temperature at the brown adipose tissue (BAT) before and after cold stimulation was observed and the expression of lipid metabolism-related genes in the liver and BAT tissues was detected to clarify the mechanism of action of AQS.</div></div><div><h3>Results</h3><div>Network pharmacology analysis showed that AQS was involved in the regulation of liver and adipose tissue function under obesity. Pathological and biochemical results showed that AQS reduced lipid accumulation in the liver and adipose tissue induced by an unhealthy diet. With the increase of cold tolerance, the volume and weight of BAT were increased by AQS, suggesting that it regulated the body heat production dominated by BAT. After AQS treatment, the levels of genes related to uncoupling protein1 (UCP1)-mediated adaptive thermogenesis in BAT tissues and lipid metabolism in the liver were also increased, which further proved that AQS activated BAT function to promote lipid metabolism in the whole body.</div></div><div><h3>Conclusion</h3><div>This study revealed the pharmacological effects of AQS, thereby providing a scientific basis for regulating BAT thermogenesis and liver lipid metabolism to alleviate obesity and providing clues for further exploring the application of natural active ingredients in the treatment of metabolism-related diseases.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 488-499"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Riboflavin metabolism involved into comparison of therapeutic action of wild-simulated and transplanted Astragali Radix to treat chronic atrophic gastritis rats based on endogenous-xenobiotics metabolism","authors":"Kaidi Fan ,&nbsp;Xu Lian ,&nbsp;Weicong Zhang ,&nbsp;Xuemei Qin ,&nbsp;Yuetao Liu","doi":"10.1016/j.chmed.2025.03.006","DOIUrl":"10.1016/j.chmed.2025.03.006","url":null,"abstract":"<div><h3>Objective</h3><div><em>Astragali Radix</em> (AR, Huangqi in Chinese) has been utilized generally as a bulk drug for the treatment of chronic atrophic gastritis (CAG) in China. The efficacy of wild-simulated AR (WAR) and transplanted AR (TAR) commercially available is unclear. This study aimed to further clarify the therapeutic action of WAR and TAR to treat CAG rats based on endogenous-xenobiotics metabolism.</div></div><div><h3>Methods</h3><div>Ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometer (UHPLC-Q-TOF/MS) based metabolomics had been used to analyze the cecal contents metabolic features and metabolic process differences of two ARs in the treatment of CAG.</div></div><div><h3>Results</h3><div>Apparent pharmacodynamic indicator examination revealed that the WAR group had a more substantial curative effect. Metabolomics studies revealed that seven endogenous metabolites were significant differences in two ARs. Furthermore, following treatment, 77 and 65 xenobiotics metabolites were identified in the WAR and TAR groups, respectively. Finally, the most critical riboflavin metabolic route in the formation of CAG was chosen for molecular docking with the relevant exogenous components, and WAR scored higher than TAR.</div></div><div><h3>Conclusion</h3><div>In this work, we analyzed the efficacy differences of AR from diverse cultivation forms by combining metabolomics and medicinal chemistry technologies, and it gave a fresh perspective for TCM efficacy evaluation and quality control research.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 513-528"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications and challenges of DNA barcoding in rapid radiation groups: Rhodiola (Crassulaceae) as a case study","authors":"Jinxin Liu ,&nbsp;Erhuan Zang ,&nbsp;Yu Tian ,&nbsp;Xinyi Li ,&nbsp;Tianyi Xin ,&nbsp;Lingchao Zeng ,&nbsp;Lijia Xu ,&nbsp;Peigen Xiao","doi":"10.1016/j.chmed.2024.08.001","DOIUrl":"10.1016/j.chmed.2024.08.001","url":null,"abstract":"<div><h3>Objective</h3><div><em>Rhodiolae Crenulatae Radix</em> et <em>Rhizoma</em> (Hongjingtian in Chinese, RCRR), the roots and rhizomes of <em>Rhodiola crenulata</em> and its application in the medicinal market is very chaotic. In this study, DNA barcoding database and identification engine of <em>Rhodiola</em> species were established, decoction pieces from the medicinal market were identified, and the application and challenges of DNA barcoding in the rapid radiation of <em>Rhodiola</em> species were analyzed. This study provides reference for the protection, rational development, and utilization of endangered resources within <em>Rhodiola</em> species.</div></div><div><h3>Methods</h3><div>A total of 50 original plant samples from 20 species of the genus <em>Rhodiola</em> from Hebei, Xinjiang, Tibet, Jilin, and other major production areas were collected. Theses samples cover the typical distribution area (Qinghai-Tibetan Platea) of <em>Rhodiola</em> species and other scattered alpine regions (Changbai Mountain, Taibai Mountain, Lushan Mountain, etc.), it encompasses all <em>Rhodiola</em> species with thick rhizomes in China. ITS2 and <em>psbA-trnH</em> barcode of <em>Rhodiola</em> database (BORD) were established and an identification engine named <em>Rhodiola</em>-IDE was developed. The stability and accuracy of the standard DNA barcoding database were evaluated using two datasets. <em>Rhodiola</em>-IDE identified 31 decoction pieces of <em>RCRR</em> from the medicinal material market.</div></div><div><h3>Results</h3><div>The BORD containing 1 532 sequences of 88 <em>Rhodiola</em> species has been established, and the identification efficiency results showed good accuracy and stability. According to the <em>Chinese Pharmacopoeia</em> (2020 edition), 23 samples (74.2%) were identified as authentic <em>R. crenulata</em>, while the rest of the marketed varieties were <em>R. kirilowii</em>, <em>R. dumulosa</em>, and <em>R. fastigiata</em>. The product label “Larger flower, Hongjingtian” was identified as <em>R. crenulata</em>. Samples labeled as “Smaller flower, Hongjingtian” were identified as <em>R. crenulata, R. kirilowii</em>, and <em>R. fastigiata</em>.</div></div><div><h3>Conclusion</h3><div>ITS2 and <em>psbA-trnH</em> barcodes can identify monophyletic groups represented by <em>R. crenulata.</em> However, for non-monophyletic species, it is necessary to collect as many samples as possible and combine them with multiple markers for joint identification. This study discussed the application and challenges of DNA barcodes in <em>Rhodiola</em> under rapid radiation conditions, providing a scientific basis for the rational development and utilization of <em>Rhodiola</em> varieties.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 555-561"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qingjie Fuzheng Granule prevents colitis-associated colorectal cancer by inhibiting abnormal activation of NOD2/NF-κB signaling pathway mediated by gut microbiota disorder","authors":"Bin Huang ,&nbsp;Honglin An ,&nbsp;Mengxuan Gui ,&nbsp;Yiman Qiu ,&nbsp;Wen Xu ,&nbsp;Liming Chen ,&nbsp;Qiang Li ,&nbsp;Shaofeng Yao ,&nbsp;Shihan Lin ,&nbsp;Tatyana Aleksandrovna Khrustaleva ,&nbsp;Ruiguo Wang ,&nbsp;Jiumao Lin","doi":"10.1016/j.chmed.2025.04.001","DOIUrl":"10.1016/j.chmed.2025.04.001","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates the efficacy and mechanisms of Qingjie Fuzheng Granules (QFG) in inhibiting colitis-associated colorectal cancer (CAC) development via RNA sequencing (RNA-seq) and 16S ribosomal RNA (rRNA) correlation analysis.</div></div><div><h3>Methods</h3><div>CAC was induced in BALB/c mice using azoxymethane (AOM) and dextran sulfate sodium (DSS), and QFG was administered orally to the treatment group. The effects of QFG on CAC were evaluated using disease index, histology, and serum T-cell ratios. RNA-seq and 16S rRNA analysis assessed the transcriptome and microbiome change. Key pharmacodynamic pathways were identified by integrating these data and confirmed via Western blotting and immunofluorescence. The link between microbiota and CAC-related markers was explored using linear discriminant analysis effect size and Spearman correlation analysis.</div></div><div><h3>Results</h3><div>Long-term treatment with QFG prevented AOM/DSS-induced CAC formation, reduced levels of interleukin (IL)-1<em>β</em>, tumor necrosis factor-alpha (TNF-<em>α</em>), IL-6, and interferon <em>γ</em> (IFN-<em>γ</em>), and increased CD3⁺ and CD4⁺/CD8⁺ T cells ratio, without causing hepatic or renal toxicity. A 16S rRNA analysis revealed that QFG rebalanced the Firmicutes/Bacteroidetes ratio and mitigated AOM/DSS-induced microbiota disturbances. Transcriptomics and Western blotting analysis identified the nucleotide-binding oligomerization domain-containing protein 2 (NOD2<em>)</em>/nuclear factor kappa-B (NF-<em>κ</em>B) pathway as key for QFG’s treatment against CAC. Furthermore, QFG decreased the abundance of Bacilli, Bacillales, Staphylococcaceae, <em>Staphylococcus</em>, Lactobacillales, <em>Aerococcus</em>, <em>Alloprevotella</em>, and <em>Akkermansia</em>, while increasing Clostridiales, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Ruminococcaceae, and Muribaculaceae, which were highly correlated with CAC-related markers or NOD2/NF-<em>κ</em>B pathway.</div></div><div><h3>Conclusion</h3><div>By mapping the relationships between CAC, immune responses, microbiota, and key pathways, this study clarifies the mechanism of QFG in inhibiting CAC, highlighting its potential for clinical use as preventive therapy.</div></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"17 3","pages":"Pages 500-512"},"PeriodicalIF":4.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}