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Effect of environmental factors on the control of milky disease in Chinese mitten crab (Eriocheir sinensis) 环境因素对中华绒螯蟹乳糜病防治的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-08 DOI: 10.1016/j.aquaculture.2025.742942

Rui Zhang , Zhigang Zhao , Kun Guo , Xu Zhao , Xiangyi Deng , Shihui Wang , Liang Luo

“Milk disease”, a relatively serious disease with a high infection and mortality rate, frequently threatens the aquaculture of the Chinese mitten crab area, especially in northern China in recent years. To explore effective measures on the “milky disease” control, gradient temperature and pH were designed for the aquaculture of the infected crabs according to the reproductive characteristics of the pathogens. Then, the tissue slice and the immune capacity were detected to analyze the treatment resulting response on the organism immune system; and the bacterial and fungal flora distribution of the hepatopancreas was analyzed to judge the trend of disease development. The results found that low temperature (16 °C) significantly increased the survival percentage of the infected crabs (p < 0.05), which weakened the damage of the immune system and enhanced immunoenzyme activities (p < 0.05), but without any significant change in the microbial flora structure, indicating low temperature delayed the onset of disease and can effectively gain time for further treatment and cure measures. High temperature decreased the survival percentage (p < 0.05), but the only survived crabs showed a much higher immunocompetence and a reduced percent of community abundance of the pathogen in vivo (p < 0.05). These crabs prompted the characteristics of the “cured” crabs and suggested immunity promotion and the competition/inhibition of the pathogen in vivo would be significant for the prophylaxis and treatment of the milky disease. The survival percentage and fluctuant enzyme activities of alkali-pH groups manifested that alkali pH brought a positive response in the preliminary stage but damaged the immune system of the infected crabs, indicating it is essential to reappraise the adaptability to environmental factors in the infected crabs for disease control. This study is of crucial meaning for establishing new strategies for prevention and control of the “milky disease”.

近年来，“乳病”是一种感染率高、死亡率高的严重病害，频繁威胁着中华绒螯蟹养殖区，特别是北方地区的养殖。为探索控制“乳糜病”的有效措施，根据病原菌的繁殖特点，设计了受感染蟹的养殖温度和pH梯度。然后，检测组织切片和免疫能力，分析治疗对机体免疫系统的影响；分析肝胰脏细菌和真菌菌群分布，判断疾病发展趋势。结果发现，低温（16℃）显著提高了感染蟹的成活率(p <；0.05)，可减轻免疫系统损伤，增强免疫酶活性(p <；0.05)，但微生物菌群结构无明显变化，说明低温延缓了疾病的发病，可以有效地为进一步的治疗和治愈措施争取时间。高温降低了成活率(p <；0.05)，但唯一存活的螃蟹表现出更高的免疫能力和体内病原体群落丰度的百分比降低(p <；0.05)。这些螃蟹提示了“治愈”螃蟹的特征，提示免疫促进和体内病原体的竞争/抑制对预防和治疗乳糜病有重要意义。碱-pH组的存活率和酶活性波动表明，碱-pH在前期能引起感染蟹的积极反应，但对感染蟹的免疫系统造成损害，说明重新评价感染蟹对环境因素的适应性对疾病控制有重要意义。本研究对制定“乳糜泻”防治新策略具有重要意义。

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引用次数: 0

Impacts of present heat waves in freshwater environments during out-growth phase in rainbow trout, Oncorhynchus mykiss, underscores the need of new wisely designed mitigation strategies: climatological, physiological and molecular analyses 目前的热浪对虹鳟生长阶段淡水环境的影响，强调需要新的明智设计的缓解策略：气候，生理和分子分析

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-06-23 DOI: 10.1016/j.aquaculture.2025.742879

Marta F. Riesco , Laura Calvo-Rodríguez , Maria Ángeles Esteban , Paulino de Paz , Ignacio Fernández

Extreme temperature events are urgent concerns for fish farming sustainability. Only scarce studies have focused on the potential effects of heat waves (HW). Here, the incidence of HW in a freshwater basin and the related abiotic conditions (dissolved oxygen content, water flow rate and water level) in 17 different locations of the major rainbow trout (Oncorhynchus mykiss) production region in Spain (Castilla y León), have been explored during the last 4 years. Results showed that, HW duration progressively increased (up to 71 days), mean intensities (I_mean) ranged 0.56–5.47 °C, and maximum intensities (I_max) reached up to 8.94 °C. Based on this, the growth performance and the biochemical and molecular responses at systemic and local levels in rainbow trout specimens during the out-growth phase (approx. 29 cm and 350 g) have been assessed when exposed to a conservative model of single HW lasting 19 days, with an I_mean of 4.18, and an I_max of 6.9 and (HW group), or maintained at 14.5 °C (Control group). Specimens exposed to this HW event in the freshwater showed different responses depending on the parameter and level considered. In exposed specimens, body weight tended to decrease, while malondialdehyde (MDA) content in skin mucus increased. Blood plasma activities of superoxide dismutase and glutathione peroxidase were also increased in HW specimens. Finally, although HW exposure didn't affect the expression of redox balance genes at gills and liver, the expression of particular heat shock related genes was up-regulated. Considering current and future climate change scenarios, higher environmental monitoring effort should be done for wise decision-making on operational activities (e.g., better selection of locations, improved rearing system designs and further adapted husbandry practices, preference for resistant fish strains, and/or implementing better systems for monitoring and weather forecasting) by fish farm managers to cope with HW events. Specifically, climatology data on incidence, intensity and duration of HW in southern Europe should be considered for strategic spatial planning for long-term continental aquaculture sustainability.

极端温度事件是鱼类养殖可持续性的紧迫问题。只有很少的研究集中在热浪的潜在影响上。在过去的4年里，我们对西班牙主要虹鳟鱼（Oncorhynchus mykiss）生产区（Castilla y León）的17个不同地点的淡水盆地中HW的发病率以及相关的非生物条件（溶解氧含量、水流速率和水位）进行了研究。结果表明，HW持续时间逐渐增加（达71 d），平均强度（Imean）为0.56 ~ 5.47°C，最大强度（Imax）达到8.94°C。在此基础上，研究了虹鳟鱼标本在生长期的生长性能以及全身和局部水平上的生化和分子反应。当暴露于单一HW保守模型持续19天，Imean为4.18，Imax为6.9和（HW组），或维持在14.5°C（对照组）时，评估了29 cm和350 g)。在淡水中暴露于这一高压事件的标本根据所考虑的参数和水平表现出不同的反应。在暴露的标本中，体重有下降的趋势，而皮肤粘液中丙二醛（MDA）含量增加。HW标本血浆超氧化物歧化酶和谷胱甘肽过氧化物酶活性也升高。最后，虽然HW暴露不影响鳃和肝脏氧化还原平衡基因的表达，但特定热休克相关基因的表达上调。考虑到当前和未来的气候变化情景，应加强环境监测工作，以便养鱼场管理人员在业务活动中做出明智决策（例如，更好地选择地点、改进饲养系统设计和进一步适应畜牧业做法、偏好抗性鱼种和/或实施更好的监测和天气预报系统），以应对HW事件。具体而言，应考虑南欧HW发生率、强度和持续时间的气候学数据，以进行战略性空间规划，以实现大陆水产养殖的长期可持续性。

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引用次数: 0

Effects of salinity, temperature and photoperiod on egg production of Pseudodiaptomus annandalei (Copepoda: Calanoida) used as live feed in Mariculture 盐度、温度及光周期对海鱼养殖中活饲料南洋假虾产蛋的影响

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-02 DOI: 10.1016/j.aquaculture.2025.742921

Neethu Kuriakose , Imelda Joseph

Copepods are essential for maintaining marine ecosystems, and nauplii of Pseudodiaptomus annandalei serves as a crucial food source for fish larvae. This study investigated how salinity, temperature, and photoperiod (Light: Dark cycle) affect the reproductive traits of this copepod in a controlled environment. Clutch size was highest at 15 ppt salinity, with an average of 18.00 ± 0.58 eggs, while it dropped significantly to 9.33 ± 0.33 at 30 ppt. The dimensions of egg sacs and the total length of adult females were also smallest at 30 ppt. Among different salinities, the shortest times to first and total maturity were recorded at 10 ppt, whereas the longest times occurred at 30 ppt. Temperature also played a critical role; clutch size peaked at 28 °C and was lowest at 25 °C, with an average of 11.00 ± 0.41. Photoperiod had a limited impact on reproductive output, but the highest clutch size was observed in a 0 hL:24 hD light cycle. The findings suggest that optimal conditions for maximizing nauplii production in P. annandalei are a salinity of 15 ppt, a temperature of 28 °C, and a continuous dark photoperiod (0 hL:24 hD). Under these conditions, the highest hatching percentage was achieved, highlighting the importance of these factors for effective breeding in this copepod species. This research provides valuable insights for marine ecology and aquaculture, emphasizing the significance of environmental conditions in copepod reproduction.

桡足类动物对海洋生态系统的维持至关重要，而南南达莱假蝶爪是鱼类幼虫的重要食物来源。本研究探讨了盐度、温度和光周期（光暗循环）在受控环境下对桡足动物生殖性状的影响。卵数在盐度为15 ppt时最高，平均为18.00±0.58枚，而在盐度为30 ppt时，卵数显著下降至9.33±0.33枚。卵囊尺寸和成年雌虫总长度在30ppt时最小。在不同的盐度中，10 ppt的时间最短，30 ppt的时间最长。温度也起了关键作用；离合器尺寸在28°C时最高，在25°C时最低，平均值为11.00±0.41。光周期对繁殖产量的影响有限，但在0 hL:24 hD光周期下观察到最高的卵窝数。结果表明，在盐度为15 ppt、温度为28°C、连续暗光周期（0 hL:24 hD）的条件下，楠楠达莱（p.a annandalei）的nauplii产量最高。在这些条件下，孵化率最高，突出了这些因素对这种桡足类物种有效繁殖的重要性。该研究为海洋生态学和水产养殖提供了有价值的见解，强调了环境条件在桡足动物繁殖中的重要性。

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引用次数: 0

Letter to the editor: Comment on development of a TGC-based growth model for the olive flounder, Paralichthys olivaceus, and its application in developing a fish growth simulator architecture 致编辑：对橄榄比目鱼（parichthys olivaceus）基于tgc的生长模型的发展及其在鱼类生长模拟器体系结构开发中的应用的评论

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-06 DOI: 10.1016/j.aquaculture.2025.742924

Jianpeng Yu, Baochao Liao

引用次数: 0

Genome-wide analysis of carotenoid accumulation related genes reveals key genes underlying ovarian color divergence in bivalves: Insights from Chlamys nobilis and Crassostrea gigas 类胡萝卜素积累相关基因的全基因组分析揭示了双壳类动物卵巢颜色差异的关键基因：来自Chlamys nobilis和长牡蛎的见解

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-05 DOI: 10.1016/j.aquaculture.2025.742929

Huiqi Li , Changping Xu , Yifan Li , Xuanbing Wu , Meizhen Chen , Beiyu Yan , Yaoting Liu , Taixin Chen , Caixuan Wang , Hongkuan Zhang , Huaiping Zheng

Gonadal color polymorphism is a widespread phenomenon in marine species. It has critical implications for health assessment, reproductive signaling, and environmental adaptation. Carotenoids, lipid-soluble pigments with antioxidant and immunomodulatory functions, play central roles in gonadal pigmentation. However, the genetic mechanisms underlying interspecific divergence in gonadal coloration remain poorly understood, particularly in bivalves. In this study, we conducted a comprehensive genomic and transcriptomic analysis of five carotenoid-related gene families—scavenger receptor class B (SR-B), vitellogenin (Vg), glutathione S-transferase P (GSTP), steroidogenic acute regulatory protein (STARD3), and carotenoid cleavage dioxygenase (CCD)-across 18 bivalve species, comprising 3 Mytilidae, 7 Ostreidae, and 8 Pectinidae. Phylogenetic and gene family contraction/expansion analyses revealed significant expansion of CCD genes in scallops and conservation of Vg and SR-B genes across bivalves. Tissue expression analysis showed that Vg and GSTP were predominantly expressed in the ovaries of scallops (Chlamys nobilis), with CnVG2 and CnGSTP1 exhibiting striking female-biased expression. In contrast to scallops, oysters (Crassostrea gigas) showed limited sexual dimorphism in these genes. Expression analysis of ovarian developmental stage revealed that Vg and GSTP were significantly upregulated during ovarian maturation in scallops, and no significant changes in CCD expression, suggesting a “high uptake, low degradation” model driving carotenoid accumulation in scallops. Collectively, these findings highlight the divergent roles of carotenoid-related genes in scallops and oysters, with scallops utilizing these genes for vivid ovarian pigmentation and oysters repurposing them for stress resilience. This study provides new insights into the genetic basis of gonadal color divergence in bivalves and potential molecular targets for optimizing reproductive strategy in aquaculture.

性腺颜色多态性是海洋物种中普遍存在的现象。它对健康评估、生殖信号和环境适应具有重要意义。类胡萝卜素是一种具有抗氧化和免疫调节功能的脂溶性色素，在性腺色素沉着中起着重要作用。然而，性腺着色的种间差异的遗传机制仍然知之甚少，特别是在双壳类动物中。在这项研究中，我们对18种双壳类动物进行了5个类胡萝卜素相关基因家族的全面基因组和转录组学分析，包括3种Mytilidae， 7种Ostreidae和8种Pectinidae，包括清净受体B类（SR-B），卵黄原蛋白（Vg），谷胱甘肽s -转移酶P (GSTP)，类固醇急性调节蛋白（STARD3）和类胡萝卜素裂解双加氧酶（CCD）。系统发育和基因家族收缩/扩增分析表明，CCD基因在扇贝中显著扩增，而Vg和SR-B基因在双壳类动物中保存。组织表达分析表明，Vg和GSTP主要在栉孔扇贝（Chlamys nobilis）的卵巢中表达，而CnVG2和CnGSTP1表现出明显的雌性偏向性表达。与扇贝相比，牡蛎（长牡蛎）在这些基因中表现出有限的性别二态性。卵巢发育阶段的表达分析显示，在扇贝卵巢成熟过程中，Vg和GSTP的表达显著上调，而CCD的表达无显著变化，提示扇贝体内类胡萝卜素的积累存在“高摄取、低降解”的模式。总的来说，这些发现突出了类胡萝卜素相关基因在扇贝和牡蛎中的不同作用，扇贝利用这些基因来实现生动的卵巢色素沉着，而牡蛎则利用它们来实现应激恢复。该研究为双壳类动物性腺颜色分化的遗传基础和优化水产养殖繁殖策略的潜在分子靶点提供了新的见解。

{"title":"Genome-wide analysis of carotenoid accumulation related genes reveals key genes underlying ovarian color divergence in bivalves: Insights from Chlamys nobilis and Crassostrea gigas","authors":"Huiqi Li , Changping Xu , Yifan Li , Xuanbing Wu , Meizhen Chen , Beiyu Yan , Yaoting Liu , Taixin Chen , Caixuan Wang , Hongkuan Zhang , Huaiping Zheng","doi":"10.1016/j.aquaculture.2025.742929","DOIUrl":"10.1016/j.aquaculture.2025.742929","url":null,"abstract":"<div><div>Gonadal color polymorphism is a widespread phenomenon in marine species. It has critical implications for health assessment, reproductive signaling, and environmental adaptation. Carotenoids, lipid-soluble pigments with antioxidant and immunomodulatory functions, play central roles in gonadal pigmentation. However, the genetic mechanisms underlying interspecific divergence in gonadal coloration remain poorly understood, particularly in bivalves. In this study, we conducted a comprehensive genomic and transcriptomic analysis of five carotenoid-related gene families—scavenger receptor class B (<em>SR-B</em>), vitellogenin (<em>Vg</em>), glutathione S-transferase P (<em>GSTP</em>), steroidogenic acute regulatory protein (<em>STARD3</em>), and carotenoid cleavage dioxygenase (<em>CCD</em>)-across 18 bivalve species, comprising 3 <em>Mytilidae</em>, 7 <em>Ostreidae</em>, and 8 <em>Pectinidae</em>. Phylogenetic and gene family contraction/expansion analyses revealed significant expansion of <em>CCD</em> genes in scallops and conservation of <em>Vg</em> and <em>SR-B</em> genes across bivalves. Tissue expression analysis showed that <em>Vg</em> and <em>GSTP</em> were predominantly expressed in the ovaries of scallops (<em>Chlamys nobilis</em>), with <em>CnVG2</em> and <em>CnGSTP1</em> exhibiting striking female-biased expression. In contrast to scallops, oysters (<em>Crassostrea gigas</em>) showed limited sexual dimorphism in these genes. Expression analysis of ovarian developmental stage revealed that <em>Vg</em> and <em>GSTP</em> were significantly upregulated during ovarian maturation in scallops, and no significant changes in <em>CCD</em> expression, suggesting a “high uptake, low degradation” model driving carotenoid accumulation in scallops. Collectively, these findings highlight the divergent roles of carotenoid-related genes in scallops and oysters, with scallops utilizing these genes for vivid ovarian pigmentation and oysters repurposing them for stress resilience. This study provides new insights into the genetic basis of gonadal color divergence in bivalves and potential molecular targets for optimizing reproductive strategy in aquaculture.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"610 ","pages":"Article 742929"},"PeriodicalIF":3.9,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell RNA sequencing reveals the immune cell landscape of head kidney in the Perciform fish (Micropterus salmoides) 单细胞RNA测序揭示了Perciform鱼头部肾脏免疫细胞景观

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-01 DOI: 10.1016/j.aquaculture.2025.742918

Zheng-Yang Zhou , Chen-Wang Lu , Zheng-Ran Jian , Shang-Jie Bai , Lin-Feng Kuang , Jin-Lai Gu , Ming-Zhu Liu , Peng-Fei Li , Gao-Xue Wang , Qing Yu , Fei Ling , Jorge Galindo-Villegas , Tao Liu

Teleost fish, a key transitional group in vertebrate evolution, possess immune systems that diverge substantially from those of mammals in structure, composition, and function. Within this group, the order Perciformes stands out for its ecological breadth, evolutionary significance, and value as a model for immunological research. However, the cellular complexity and developmental dynamics of their immune system remain insufficiently characterized. In this study, we employed single-cell RNA sequencing (scRNA-seq) to construct a comprehensive transcriptomic atlas of the head kidney in largemouth bass (Micropterus salmoides), a representative perciform species. We identified and annotated eight major hematopoietic and stromal cell populations, including hematopoietic stem/progenitor cells (HSPCs), erythrocytes (Eryt), granulocytes (Gran), macrophages (Mac), dendritic cells (DC), T and B cells, and mesenchymal stromal cells (MSCs). Subsequent analyses revealed extensive subpopulation heterogeneity across these lineages. We delineated early progenitor subsets, mapped B cell developmental trajectories, and characterized functionally distinct Mac and T cell subtypes. Notably, we identified Eryt cells exhibiting high MHC class II expression, supporting immunological roles beyond oxygen transport. A dot plot of core erythrocyte genes revealed largely lineage-restricted expression. However, several hemoglobin paralogs were also expressed in non-erythroid cells, suggesting functional divergence. Additionally, we identified a putative Gran-erythroid progenitor population and Mac subsets with distinct transcriptional profiles, underscoring the complexity, potential functionality, and wide diversity of specialized immune cell dynamics in Perciformes. Together, these findings significantly advance our understanding of teleost immune cell diversity and lineage specialization. The generated cell atlas provides a valuable resource and novel perspectives for deciphering fish immunity and hematopoiesis, advancing comparative immunology, and informing aquaculture health management.

硬骨鱼是脊椎动物进化过程中一个重要的过渡群体，其免疫系统在结构、组成和功能上与哺乳动物有很大的不同。在这一组中，表现目因其生态广度、进化意义和作为免疫学研究模型的价值而脱颖而出。然而，他们的免疫系统的细胞复杂性和发育动力学仍然没有充分表征。在本研究中，我们采用单细胞RNA测序（scRNA-seq）技术构建了大口黑鲈（Micropterus salmoides）头部肾脏的综合转录组图谱。我们鉴定并注释了8种主要的造血和基质细胞群，包括造血干细胞/祖细胞（HSPCs）、红细胞（Eryt）、粒细胞（Gran）、巨噬细胞（Mac）、树突状细胞（DC）、T细胞和B细胞以及间充质基质细胞（MSCs）。随后的分析揭示了这些谱系中广泛的亚群异质性。我们描绘了早期祖细胞亚群，绘制了B细胞发育轨迹，并描述了功能上不同的Mac和T细胞亚型。值得注意的是，我们发现Eryt细胞表现出高MHC II类表达，支持氧运输以外的免疫作用。红细胞核心基因的点图显示了主要的谱系限制性表达。然而，在非红系细胞中也表达了几种血红蛋白相似物，表明功能上存在差异。此外，我们确定了一个假定的大红系祖细胞群和具有不同转录谱的Mac亚群，强调了Perciformes中特化免疫细胞动力学的复杂性、潜在功能和广泛多样性。总之，这些发现显著地促进了我们对硬骨鱼免疫细胞多样性和谱系特化的理解。生成的细胞图谱为破译鱼类免疫和造血、推进比较免疫学和水产养殖健康管理提供了宝贵的资源和新的视角。

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引用次数: 0

Market dynamics and E-commerce satisfaction in China's aquatic food sector: Machine learning and data insights 中国水产食品行业的市场动态和电子商务满意度：机器学习和数据洞察

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-06-30 DOI: 10.1016/j.aquaculture.2025.742904

Mausam Budhathoki , Lincen Li , Hao Xu , Wenbo Zhang , Xuzhou Ma , Sujita Pandey , Marianne Thomsen

China's growing demand for aquatic foods, driven by the rapid expansion of e-commerce, is reshaping global seafood markets and influencing the broader sustainable food system. This transformation is altering domestic market dynamics and creating new challenges and opportunities in international trade, sustainable supply chains, and market competitiveness. This study investigates production trends, import reliance, and consumer e-commerce satisfaction in the aquatic food market, with a focus on implications for sustainable supply chain policy and management. By employing supervised machine learning technique, Support Vector Machines (SVM), along with Latent Dirichlet Allocation (LDA), Mann-Whitney U test, and logistic regression, the study uncovers critical insights into consumer preferences, including the factors driving e-commerce satisfaction, and supply chain dynamics, such as the impact of product freshness and delivery efficiency.

The study highlights the growing reliance on imports in China's aquatic food market, despite expanding domestic production. Fresh and frozen products, particularly crustaceans and finfish, consistently receive higher consumer satisfaction on e-commerce platforms, primarily due to their quality and freshness. Domestic producers face increasing pressure to meet these high standards, particularly in freshness, quality control, and delivery efficiency. The findings point to challenges in meeting consumer expectations and suggest opportunities for policy interventions to enhance the competitiveness of domestic aquatic food products. The research highlights the transformative role of e-commerce platforms in shaping China's aquatic food market, providing actionable insights for policymakers, producers, and e-commerce stakeholders to navigate emerging trends, improve supply chain efficiency, and meet evolving consumer demands.

在电子商务快速扩张的推动下，中国对水产食品的需求不断增长，正在重塑全球海鲜市场，并影响更广泛的可持续食品体系。这种转变正在改变国内市场动态，并在国际贸易、可持续供应链和市场竞争力方面创造新的挑战和机遇。本研究探讨水产食品市场的生产趋势、进口依存度和消费者电子商务满意度，并重点探讨可持续供应链政策和管理的启示。通过使用监督机器学习技术，支持向量机（SVM），以及潜在狄利克雷分配（LDA），曼-惠特尼U检验和逻辑回归，该研究揭示了消费者偏好的关键见解，包括驱动电子商务满意度的因素，以及供应链动态，如产品新鲜度和交付效率的影响。该研究强调，尽管中国国内生产不断扩大，但中国水产食品市场对进口的依赖越来越大。新鲜和冷冻产品，尤其是甲壳类和鱼类，在电商平台上一直获得较高的消费者满意度，主要是因为它们的质量和新鲜度。国内生产商面临着越来越大的压力，要达到这些高标准，特别是在新鲜度、质量控制和交货效率方面。调查结果指出了满足消费者期望的挑战，并提出了政策干预的机会，以提高国内水产食品的竞争力。该研究强调了电子商务平台在塑造中国水产食品市场方面的变革性作用，为政策制定者、生产者和电子商务利益相关者提供了可操作的见解，以引导新兴趋势，提高供应链效率，满足不断变化的消费者需求。

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引用次数: 0

Transcription of immune genes in ranched southern bluefin tuna show correlations to Cardicola spp. (Digenea: Aporocotylidae) infection 南部养殖蓝鳍金枪鱼免疫基因转录与Cardicola spp. （Digenea: Aporocotylidae）感染相关

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-06-24 DOI: 10.1016/j.aquaculture.2025.742877

Maree Widdicombe , Sarah Eastwood , Bronwyn E. Campbell , Cecilia Power , Barbara F. Nowak , Paul A. Ramsland , Nathan J. Bott

Cardicola forsteri and C. orientalis (Digenea: Aporocotylidae) are significant pathogens affecting the Southern bluefin tuna (SBT), Thunnus maccoyii, aquaculture industry. The aims of this research were to identify a relationship between immune gene expression of SBT and C. forsteri and C. orientalis infection burdens; and to understand how immune gene expression changes over time during commercial ranching. SBT were collected during commercial operations in 2022 after 4, 8 and 10 weeks of ranching and the transcription of interleukin 1 beta (il1b), interleukin 8 (il8), tumour necrosis factor 2 (tnf2), immunoglobulin m (igm), major histocompatibility complex 2 (mhc2) and t cell receptor beta (tcrb) were measured from the anterior kidney and gills using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Cardicola forsteri and C. orientalis infection was quantified as number of eggs/mm of SBT gill filament, number of adult flukes in SBT heart and C. forsteri and C. orientalis ITS-2 rDNA copy number/mg in SBT gills. Expression of il8 and igm in anterior kidney was positively correlated to C. forsteri and C. orientalis eggs/mm of SBT gill filament and to the copy number of C. forsteri ITS-2 rDNA in SBT gills. Expression of igm and mhc2 was higher in SBT positive for adult flukes and the expression of igm, mhc2 and tcrb in the anterior kidney was positively correlated to the number of adult flukes in SBT heart. Expression of tcrb in gills was negatively correlated to the number of adult flukes in SBT heart and negatively correlated to the copy number of C. orientalis ITS-2 rDNA. Expression of the adaptive immune genes igm, mhc2 and tcrb increased over the ranching duration in the kidney of SBT and the innate immune genes il1b and il8 decreased over the ranching duration in SBT gills. Despite evidence of an immune response to C. forsteri and C. orientalis, SBT were not able to clear infections during the standard industry ranching timeline.

Cardicola forsteri和C. orientalis （Digenea: Aporocotylidae）是影响南方蓝鳍金枪鱼（SBT）、鲭鱼（Thunnus maccoyii）水产养殖业的重要病原体。本研究的目的是确定SBT免疫基因表达与福氏弧菌和东方弧菌感染负荷之间的关系；并了解在商业牧场期间免疫基因表达如何随时间变化。饲养4、8和10周后，于2022年商业手术中收集SBT，采用逆转录定量聚合酶链式反应（RT-qPCR）检测前肾和鳃中白细胞介素1 β (il1b)、白细胞介素8 （il8）、肿瘤坏死因子2 （tnf2）、免疫球蛋白m （igm）、主要组织相容性复合体2 （mhc2）和t细胞受体β (tcrb)的转录。以SBT鳃丝虫卵数/mm、SBT心脏成虫吸虫数、SBT鳃中forsteri和东方弓形虫ITS-2 rDNA拷贝数/mg为感染指标。前肾il8和igm的表达与SBT鳃丝中forsteri和orientalis卵数/mm及SBT鳃中forsteri ITS-2 rDNA拷贝数呈正相关。igm和mhc2的表达在SBT阳性的成年吸虫中较高，igm、mhc2和tcrb在前肾中的表达与SBT心脏中成年吸虫的数量呈正相关。鳃中tcrb的表达与SBT心脏吸虫成虫数呈负相关，与东方鱼ITS-2 rDNA拷贝数呈负相关。适应性免疫基因igm、mhc2和tcrb在SBT肾脏的表达随放牧时间的延长而增加，先天免疫基因il1b和il8在SBT鳃中的表达随放牧时间的延长而降低。尽管有证据表明对福氏弧菌和东方弧菌有免疫反应，但在标准的工业牧场时间内，SBT无法清除感染。

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引用次数: 0

Achievement of prosperous nitrification cycle fosters diversity in freshwater microbiome within recirculating aquaculture systems after adjustment period 经过调整期后，实现繁荣的硝化循环可促进循环水养殖系统内淡水微生物群的多样性

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-07-08 DOI: 10.1016/j.aquaculture.2025.742941

Jiho Yang , Junhyuk Seo , Jimin Choi , Yehyeon Cha , Yunhyeok Jang , Ju-Ae Hwang , Seung-Yoon Oh

In recirculating aquaculture systems (RAS), a significant challenge involves managing the biological cycling of nitrogenous waste. As a result, there is worldwide focus on the diversity of biological groups involved in nitrogen cycling and their impacts. However, there is still a lack of information concerning the microbial diversity, particularly in relation to biological nitrogen cycling. We hypothesized that biological nitrification in RAS becomes fully established only after a distinct microbial adjustment period following microbial inoculant (MI) treatment, and that this phase features distinct shifts in microbiome composition and nitrogen compound dynamics. To test this, we monitored water chemistry and microbial community structure over an 8-week pre-treatment maturation phase in freshwater RAS for Japanese eel (Anguilla japonica) cultivation. Using 16S rRNA gene amplicon sequencing and qPCR targeting ammonia- and nitrite-oxidizing bacteria, we found that nitrifier abundance increased sharply from week 3 post-treatment. Random forest analysis and structural equation modeling revealed that nitrate levels—serving as a proxy for complete nitrification—had the strongest direct influence on microbiome diversity. Diversity indices and beta-dispersion analyses confirmed that microbial communities during the adjustment period (weeks 0–3) were significantly different from those in the stabilization phase (weeks 4–8). These findings demonstrate the existence of a critical, temporally defined adjustment phase in RAS maturation, during which microbiome structure and nitrification processes co-develop. Our study provides mechanistic insights into microbiome assembly in RAS and highlights the importance of temporal monitoring in optimizing MI-based water treatment strategies.

在循环型水产养殖系统（RAS）中，一项重大挑战涉及管理含氮废物的生物循环。因此，参与氮循环的生物类群的多样性及其影响受到了全世界的关注。然而，关于微生物多样性，特别是与生物氮循环有关的信息仍然缺乏。我们假设RAS中的生物硝化作用只有在微生物接种剂（MI）处理后经过一段不同的微生物调整期才能完全建立，并且这一阶段的微生物组组成和氮化合物动力学发生了明显的变化。为了验证这一点，我们在日本鳗鲡（Anguilla japonica）养殖的淡水RAS中监测了8周预处理成熟阶段的水化学和微生物群落结构。利用16S rRNA基因扩增子测序和针对氨氧化菌和亚硝酸盐氧化菌的qPCR，我们发现从处理后第3周开始，硝化菌丰度急剧增加。随机森林分析和结构方程模型表明，硝酸盐水平作为完全硝化的代表，对微生物群落多样性具有最强的直接影响。多样性指数和β -分散度分析证实，调整期（0-3周）的微生物群落与稳定化期（4-8周）存在显著差异。这些发现表明，在RAS成熟过程中存在一个关键的、暂时确定的调整阶段，在此期间，微生物组结构和硝化过程共同发展。我们的研究提供了RAS中微生物组组装的机制见解，并强调了时间监测在优化基于mi的水处理策略中的重要性。

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引用次数: 0

Magnetic resonance imaging for rapid, noninvasive and accurate assessment of ovarian maturation in live Channa argus (Cantor, 1842) 磁共振成像用于快速、无创、准确评估活鳢卵巢成熟（Cantor, 1842）

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture

Pub Date : 2026-10-30 Epub Date: 2025-06-23 DOI: 10.1016/j.aquaculture.2025.742872

Wenqian Sun , Weiyin Vivian Liu , Xingyu Chen , Guangcan Lin , Hao Li , Yanting Song , Chengdong Peng , Wen Xiong , Yu Peng , Shangong Wu , Shouqi Xie , Sovan Lek , Mette Sørensen , Haokun Liu , Dong Liu , Lei Pan

Current methods for the determination of ovarian maturation in fish have shortcomings. They are often time-consuming, inaccurate, and fish are sacrificed during the procedure. A novel noninvasive and accurate method to assess ovarian maturation can be to use magnetic resonance imaging (MRI). Long scanning time is a limitation for use in fish. A clinical MRI with a new sequence employing a shorter scanning time may be more feasible for the assessment of ovarian maturation in female fish. The aim of this study was to investigate ovarian maturation in Channa argus by clinical MRI with the three-dimensional fast spin echo Cube-flex (3D-FSE-Cube-Flex) T₂WI sequence, compared with two traditional sequences. The results demonstrated that compared with two traditional sequences, the 3D-FSE-Cube-Flex T₂WI sequence greatly reduced scanning time (only requiring 140 s per fish) without reducing the scanning quality. A significant correlation (R² = 0.985, p < 0.001, N = 112) was found between the percentage volume of the ovary measured by MRI and the gonadosomatic index (GSI). Besides, ovarian maturation MRI images at different development stages were captured. These findings suggest that clinical MRI with the 3D-FSE-Cube-Flex T₂WI sequence is a rapid, accurate, and noninvasive method for assessing ovarian maturation, with great potential applications in sustainable aquaculture.

目前测定鱼类卵巢成熟的方法存在不足。它们通常是耗时的，不准确的，并且在过程中会牺牲鱼。磁共振成像（MRI）是一种新的、无创的、准确的评估卵巢成熟的方法。扫描时间长是在鱼类中使用的一个限制。临床MRI采用新的序列，采用较短的扫描时间，可能更可行的评估卵巢成熟雌鱼。本研究的目的是通过三维快速自旋回声立方体-flex (3D-FSE-Cube-Flex) T2WI序列的临床MRI与两种传统序列的比较，研究鳢卵巢成熟情况。结果表明，与两种传统序列相比，3D-FSE-Cube-Flex T2WI序列在不降低扫描质量的前提下，大大缩短了扫描时间（每条鱼只需要140 s）。相关性显著(R2 = 0.985, p <；MRI测量的卵巢体积百分比与促性腺指数（GSI）之间的差异为0.001,N = 112)。同时采集不同发育阶段的卵巢成熟MRI图像。这些结果表明，临床MRI 3D-FSE-Cube-Flex T2WI序列是一种快速、准确、无创的评估卵巢成熟的方法，在可持续水产养殖中具有很大的应用潜力。

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引用次数: 0