Pub Date : 2024-07-10Epub Date: 2024-04-28DOI: 10.1016/j.scitotenv.2024.172817
Shuai You, Li Xing, Mary Lesperance, Youlian Pan, Xuekui Zhang
Shellfish poisonings have posed severe risks to human health globally. The Canadian Shellfish Sanitation Program was established in 1948 to monitor the toxin levels at shellfish harvesting sites along the coast of six provinces in Canada. Domoic acid has been a causal toxin for amnesic shellfish poisoning, and a macro-scale analysis of the temporal and spatial variation of domoic acid along Canada's coast was conducted in this study. We aggregated the toxin levels by week in blue mussel (Mytilus edulis) and soft-shell clam (Mya arenaria) samples, respectively, over a one-year scale. The subsequent application of Functional Principal Component Analysis unveiled that magnitudes of seasonal variation and peaked DA levels around early summer, spring, or mid-fall formed the largest variation in the toxin levels in blue mussels along the coastlines of British Columbia and Prince Edward Island and in soft-shell calms along those of New Brunswick and Nova Scotia. In Quebec, the DA levels were low and varied mostly in terms of the overall magnitude from spring to fall. Downstream correlation analyses in British Columbia further discovered that, at most sites, the strongest correlations were negative between precipitation as well as inorganic nutrients (including nitrate, nitrite, phosphate, and silicate) on one side and DA a few weeks afterward on the other. These findings indicated associations between amnesic shellfish poisoning and environmental stresses.
贝类中毒在全球范围内对人类健康构成了严重威胁。加拿大贝类卫生计划成立于 1948 年,目的是监测加拿大六省沿海贝类捕捞地点的毒素水平。多羧酸是导致失忆性贝类中毒的一种毒素,本研究对加拿大沿海多羧酸的时空变化进行了宏观分析。我们将蓝贻贝(Mytilus edulis)和软壳蛤(Mya arenaria)样本中的毒素水平按周汇总,时间跨度为一年。随后应用功能主成分分析揭示了季节性变化的幅度和初夏、春季或秋季中期的 DA 水平峰值构成了不列颠哥伦比亚省和爱德华王子岛沿岸蓝贻贝以及新不伦瑞克省和新斯科舍省沿岸软壳蛤毒素水平的最大变化。在魁北克省,DA 含量较低,从春季到秋季的总体水平差异较大。不列颠哥伦比亚省的下游相关性分析进一步发现,在大多数地点,降水和无机营养物质(包括硝酸盐、亚硝酸盐、磷酸盐和硅酸盐)与几周后的DA之间呈负相关。这些发现表明,失忆性贝类中毒与环境压力之间存在关联。
{"title":"Temporal and spatial variation of domoic acid along Canada's coast.","authors":"Shuai You, Li Xing, Mary Lesperance, Youlian Pan, Xuekui Zhang","doi":"10.1016/j.scitotenv.2024.172817","DOIUrl":"10.1016/j.scitotenv.2024.172817","url":null,"abstract":"<p><p>Shellfish poisonings have posed severe risks to human health globally. The Canadian Shellfish Sanitation Program was established in 1948 to monitor the toxin levels at shellfish harvesting sites along the coast of six provinces in Canada. Domoic acid has been a causal toxin for amnesic shellfish poisoning, and a macro-scale analysis of the temporal and spatial variation of domoic acid along Canada's coast was conducted in this study. We aggregated the toxin levels by week in blue mussel (Mytilus edulis) and soft-shell clam (Mya arenaria) samples, respectively, over a one-year scale. The subsequent application of Functional Principal Component Analysis unveiled that magnitudes of seasonal variation and peaked DA levels around early summer, spring, or mid-fall formed the largest variation in the toxin levels in blue mussels along the coastlines of British Columbia and Prince Edward Island and in soft-shell calms along those of New Brunswick and Nova Scotia. In Quebec, the DA levels were low and varied mostly in terms of the overall magnitude from spring to fall. Downstream correlation analyses in British Columbia further discovered that, at most sites, the strongest correlations were negative between precipitation as well as inorganic nutrients (including nitrate, nitrite, phosphate, and silicate) on one side and DA a few weeks afterward on the other. These findings indicated associations between amnesic shellfish poisoning and environmental stresses.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-07DOI: 10.1016/j.scitotenv.2024.172994
Wenfei Zhu, Jialin Shi, Hui Wang, Ying Yu, Rui Tan, Ruizhe Shen, Jun Chen, Shengrong Lou, Min Hu, Song Guo
Submicron particulate matter (PM1) poses significant risks to health risks and global climate. In this study, secondary organic aerosols (SOA) and inorganic compositions were examined for their physicochemical characteristics and evolution using high-resolution aerosol instruments in Changzhou over one-month period. The results showed that transport accompanied by regional static conditions leaded to the occurrence of heavy pollution. In addition, regional generation and local emissions also leaded to the occurrence of light and moderate pollution during the observation period in Changzhou. Organic aerosols (OA) and nitrate (NO3-) accounted for 45 % and 23 % of PM1, respectively. The increase in PM1 was dominated by the contribution of NO3- and OA. SOA was dominance in OA (63 % with 40 % MO-OOA), which was higher than primary organic aerosols (POA). Besides, photochemical reactions and the high oxidizing nature of the urban atmosphere promoted the production of OA, especially MO-OOA in Changzhou. Our results highlight that secondary particles contribute significantly to PM pollution in Changzhou, underlining the importance of controlling emissions of gaseous precursors, especially under high oxidation conditions.
亚微米颗粒物(PM1)对健康风险和全球气候构成重大威胁。本研究利用高分辨率气溶胶仪器,对常州地区为期一个月的二次有机气溶胶(SOA)和无机成分的物理化学特征和演变过程进行了研究。结果表明,区域静态条件下的传输导致了重污染的发生。此外,区域生成和本地排放也导致常州在观测期间出现轻度和中度污染。有机气溶胶(OA)和硝酸盐(NO3-)分别占 PM1 的 45% 和 23%。PM1的增加主要是由于NO3-和OA的贡献。SOA 在 OA 中占主导地位(63%,MO-OOA 占 40%),高于原始有机气溶胶(POA)。此外,光化学反应和城市大气的高氧化性促进了 OA 的生成,尤其是常州的 MO-OOA。我们的研究结果突出表明,二次颗粒物对常州的可吸入颗粒物污染有重要影响,这也强调了控制气态前体物排放的重要性,尤其是在高氧化条件下。
{"title":"Understanding secondary particles in a regional site of Yangtze River Delta: Insights from mass spectrometric measurement.","authors":"Wenfei Zhu, Jialin Shi, Hui Wang, Ying Yu, Rui Tan, Ruizhe Shen, Jun Chen, Shengrong Lou, Min Hu, Song Guo","doi":"10.1016/j.scitotenv.2024.172994","DOIUrl":"10.1016/j.scitotenv.2024.172994","url":null,"abstract":"<p><p>Submicron particulate matter (PM<sub>1</sub>) poses significant risks to health risks and global climate. In this study, secondary organic aerosols (SOA) and inorganic compositions were examined for their physicochemical characteristics and evolution using high-resolution aerosol instruments in Changzhou over one-month period. The results showed that transport accompanied by regional static conditions leaded to the occurrence of heavy pollution. In addition, regional generation and local emissions also leaded to the occurrence of light and moderate pollution during the observation period in Changzhou. Organic aerosols (OA) and nitrate (NO<sub>3</sub><sup>-</sup>) accounted for 45 % and 23 % of PM<sub>1</sub>, respectively. The increase in PM<sub>1</sub> was dominated by the contribution of NO<sub>3</sub><sup>-</sup> and OA. SOA was dominance in OA (63 % with 40 % MO-OOA), which was higher than primary organic aerosols (POA). Besides, photochemical reactions and the high oxidizing nature of the urban atmosphere promoted the production of OA, especially MO-OOA in Changzhou. Our results highlight that secondary particles contribute significantly to PM pollution in Changzhou, underlining the importance of controlling emissions of gaseous precursors, especially under high oxidation conditions.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-10DOI: 10.1016/j.scitotenv.2024.173127
Sergi González-Herrero, Marc Lemus-Canovas, Paulo Pereira
Cold regions around the world include Arctic, Antarctic and High Mountain regions featuring low temperatures, ice-covered landscapes, permafrost, and unique ecologic interrelations. These environments are among the most sensitive to climate change and are changing rapidly as the global climate gets warmer. This editorial explores the complexity of the impacts of climate change on cold regions, highlighting recent changes across Earth system. The Special Issue here presented compiles studies that explore the climate change in different cold regions from various perspectives, including paleoclimatic reconstructions, isotherm shifts and climate projections. Despite progress, significant questions remain, demanding interdisciplinary approaches to better understand the interconnected factors shaping cold regions.
{"title":"Climate change in cold regions.","authors":"Sergi González-Herrero, Marc Lemus-Canovas, Paulo Pereira","doi":"10.1016/j.scitotenv.2024.173127","DOIUrl":"10.1016/j.scitotenv.2024.173127","url":null,"abstract":"<p><p>Cold regions around the world include Arctic, Antarctic and High Mountain regions featuring low temperatures, ice-covered landscapes, permafrost, and unique ecologic interrelations. These environments are among the most sensitive to climate change and are changing rapidly as the global climate gets warmer. This editorial explores the complexity of the impacts of climate change on cold regions, highlighting recent changes across Earth system. The Special Issue here presented compiles studies that explore the climate change in different cold regions from various perspectives, including paleoclimatic reconstructions, isotherm shifts and climate projections. Despite progress, significant questions remain, demanding interdisciplinary approaches to better understand the interconnected factors shaping cold regions.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-13DOI: 10.1016/j.scitotenv.2024.173175
Marcelina Jureczko, Wioletta Przystaś
Antineoplastic drugs are not effectively removed by wastewater treatment plants, ending up in surface waters. Since these drugs can interfere with the structure and functions of DNA, they pose a potential threat to aquatic biota. Unfortunately, many chemotherapeutic agents have not been studied in an environmental context. Additionally, there is a significant lack of information about the impact of anticancer drugs on marine organisms compared to freshwater species, and most studies only focus on the toxicity of single compounds rather than considering their occurrence as complex mixtures in the environment. Therefore, the aim of this study was to evaluate the ecotoxicity of two commonly used cytostatics, bleomycin and vincristine, toward six biomodels: Pseudokirchneriella subcapitata, Phaeodactylum tricornutum, Brachionus plicatilis, Brachionus calyciflorus, Thamnocephalus platyurus, and Artemia franciscana. These selected aquatic organisms are representatives of both freshwater and marine environments and belong to different trophic levels. The pharmaceuticals were investigated both individually and in combination. Binary mixture toxicity predictions were performed according to the Response Additivity and Independent Action models. Additionally, the toxicity data obtained from these experiments were utilized for risk assessment in the context of the drugs' environmental occurrence. The results indicated that freshwater species were generally more sensitive to both tested compounds than marine organisms, with T. platyurus being the most sensitive. Based on the tests performed on this biomodel, bleomycin was categorized as extremely toxic, while vincristine was considered moderately toxic. Neither of the applied models suitably predicted binary mixture toxicity, as the combination of drugs showed additive, synergistic, and antagonistic effects, suggesting that single compound toxicity data are insufficient for predicting the aquatic toxicities of cytostatics mixtures. The environmental risk of vincristine ranged from low to high, and for bleomycin varied from moderate to high, depending on the matrices examined. Therefore, further research on drug removal is recommended.
{"title":"Toxicity toward freshwater and marine water organisms of the cytostatic drugs bleomycin and vincristine and their binary mixture.","authors":"Marcelina Jureczko, Wioletta Przystaś","doi":"10.1016/j.scitotenv.2024.173175","DOIUrl":"10.1016/j.scitotenv.2024.173175","url":null,"abstract":"<p><p>Antineoplastic drugs are not effectively removed by wastewater treatment plants, ending up in surface waters. Since these drugs can interfere with the structure and functions of DNA, they pose a potential threat to aquatic biota. Unfortunately, many chemotherapeutic agents have not been studied in an environmental context. Additionally, there is a significant lack of information about the impact of anticancer drugs on marine organisms compared to freshwater species, and most studies only focus on the toxicity of single compounds rather than considering their occurrence as complex mixtures in the environment. Therefore, the aim of this study was to evaluate the ecotoxicity of two commonly used cytostatics, bleomycin and vincristine, toward six biomodels: Pseudokirchneriella subcapitata, Phaeodactylum tricornutum, Brachionus plicatilis, Brachionus calyciflorus, Thamnocephalus platyurus, and Artemia franciscana. These selected aquatic organisms are representatives of both freshwater and marine environments and belong to different trophic levels. The pharmaceuticals were investigated both individually and in combination. Binary mixture toxicity predictions were performed according to the Response Additivity and Independent Action models. Additionally, the toxicity data obtained from these experiments were utilized for risk assessment in the context of the drugs' environmental occurrence. The results indicated that freshwater species were generally more sensitive to both tested compounds than marine organisms, with T. platyurus being the most sensitive. Based on the tests performed on this biomodel, bleomycin was categorized as extremely toxic, while vincristine was considered moderately toxic. Neither of the applied models suitably predicted binary mixture toxicity, as the combination of drugs showed additive, synergistic, and antagonistic effects, suggesting that single compound toxicity data are insufficient for predicting the aquatic toxicities of cytostatics mixtures. The environmental risk of vincristine ranged from low to high, and for bleomycin varied from moderate to high, depending on the matrices examined. Therefore, further research on drug removal is recommended.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140943665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Persistent organic pollutants (POPs), such as polychlorinated diphenyls (PCBs) and brominated diphenyl ethers (PBDEs), are ubiquitous in the pet cat's living environment and are ingested through dietary intake and environmental exposure such as house dust. Cats are known to be susceptible to chronic kidney disease (CKD) and exposure to POPs may be associated with CKD. However, no studies have been conducted on the renal accumulation and health effects of POPs in cats. The objective of this study was to elucidate the accumulation of PCBs, PBDEs, and organochlorine pesticides (OCPs) in the kidneys of domestic cats and discuss their potential impact on feline health. We report here that cats specifically accumulate POPs in their kidneys. Tissue samples were collected from the kidneys, livers, and muscles of cats and the concentrations of POPs in these tissues were analyzed in this study. The results showed that these compounds accumulated significantly higher in the kidney compared to other tissues. In addition, the ability to accumulate in the kidney was higher in cats than in other animals, suggesting that cats have a unique pattern of POPs accumulation in their kidneys, which is thought to occur because cats store a significant number of lipid droplets in the proximal tubules of the kidneys. This unique feature suggests that lipophilic POPs may accumulate in these lipid droplets during the excretory process. Accumulation of certain POPs in the kidneys causes necrosis and sloughing of renal tubular epithelial cells, which may be associated with CKD, a common disease in cats. This study provides valuable insight into understanding the renal accumulation and risk of POPs in cats and provides essential knowledge for developing strategies to protect the health and welfare of domestic cats.
{"title":"Accumulation of persistent organic pollutants in the kidneys of pet cats (Felis silvestris catus) and the potential implications for their health.","authors":"Kei Nomiyama, Rina Sato, Fuka Sato, Akifumi Eguchi","doi":"10.1016/j.scitotenv.2024.173212","DOIUrl":"10.1016/j.scitotenv.2024.173212","url":null,"abstract":"<p><p>Persistent organic pollutants (POPs), such as polychlorinated diphenyls (PCBs) and brominated diphenyl ethers (PBDEs), are ubiquitous in the pet cat's living environment and are ingested through dietary intake and environmental exposure such as house dust. Cats are known to be susceptible to chronic kidney disease (CKD) and exposure to POPs may be associated with CKD. However, no studies have been conducted on the renal accumulation and health effects of POPs in cats. The objective of this study was to elucidate the accumulation of PCBs, PBDEs, and organochlorine pesticides (OCPs) in the kidneys of domestic cats and discuss their potential impact on feline health. We report here that cats specifically accumulate POPs in their kidneys. Tissue samples were collected from the kidneys, livers, and muscles of cats and the concentrations of POPs in these tissues were analyzed in this study. The results showed that these compounds accumulated significantly higher in the kidney compared to other tissues. In addition, the ability to accumulate in the kidney was higher in cats than in other animals, suggesting that cats have a unique pattern of POPs accumulation in their kidneys, which is thought to occur because cats store a significant number of lipid droplets in the proximal tubules of the kidneys. This unique feature suggests that lipophilic POPs may accumulate in these lipid droplets during the excretory process. Accumulation of certain POPs in the kidneys causes necrosis and sloughing of renal tubular epithelial cells, which may be associated with CKD, a common disease in cats. This study provides valuable insight into understanding the renal accumulation and risk of POPs in cats and provides essential knowledge for developing strategies to protect the health and welfare of domestic cats.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated 32 persistent organic pollutants, including 9 organochlorine pesticides, 15 polychlorinated biphenyls, 6 polycyclic aromatic hydrocarbons, and 2 organophosphate pesticides in the hair samples of domestic cats and dogs living in an urban area in Samsun, Turkiye. Hair samples were collected from 35 cats and 38 dogs, grouped by sex and age (<3 or >3 years old). Samples were extracted using a liquid-liquid extraction method and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results revealed the presence of organochlorine pesticides (n = 58, 468.65 ± 92.30 ng/g), polycyclic aromatic hydrocarbons (n = 57, 15.65 ± 3.91 ng/g), polychlorinated biphenyls (n = 55, 54.11 ± 9.47 ng/g), and organophosphate pesticides (n = 25, 568.43 ± 113.17 ng/g) in the samples. PCBs 81, 118, 128, 208, and 2,4-DDE were not detected in any samples. Only one sample did not contain any of the searched compounds. Fluorene was the most frequently detected pollutant (n = 53, 72.6 %), followed by β-hexachlorocyclohexane (n = 34, 46.6 %). The highest maximum concentration was observed for hexachlorobenzene (2748.03 ng/g), followed by aldrin (2313.45 ng/g) and fenitrothion (2081.13 ng/g). Pollutant concentrations did not differ between cats and dogs, sexes, and ages (p > 0.05). This study highlights the significant threat that urban areas pose to pets, and therefore, POPs should be monitored periodically in hair and other samples. To the best of our knowledge, this is the first report to investigate POP levels in hair samples from cats and dogs in Turkiye.
{"title":"Monitoring of environmental persistent organic pollutants in hair samples of cats and dogs.","authors":"Oguzhan Yavuz, Handan Hilal Arslan, Orhan Tokur, Ozge Marangoz, Zeyno Nuhoglu Ozturk, Saima Mushtaq","doi":"10.1016/j.scitotenv.2024.173020","DOIUrl":"10.1016/j.scitotenv.2024.173020","url":null,"abstract":"<p><p>This study investigated 32 persistent organic pollutants, including 9 organochlorine pesticides, 15 polychlorinated biphenyls, 6 polycyclic aromatic hydrocarbons, and 2 organophosphate pesticides in the hair samples of domestic cats and dogs living in an urban area in Samsun, Turkiye. Hair samples were collected from 35 cats and 38 dogs, grouped by sex and age (<3 or >3 years old). Samples were extracted using a liquid-liquid extraction method and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results revealed the presence of organochlorine pesticides (n = 58, 468.65 ± 92.30 ng/g), polycyclic aromatic hydrocarbons (n = 57, 15.65 ± 3.91 ng/g), polychlorinated biphenyls (n = 55, 54.11 ± 9.47 ng/g), and organophosphate pesticides (n = 25, 568.43 ± 113.17 ng/g) in the samples. PCBs 81, 118, 128, 208, and 2,4-DDE were not detected in any samples. Only one sample did not contain any of the searched compounds. Fluorene was the most frequently detected pollutant (n = 53, 72.6 %), followed by β-hexachlorocyclohexane (n = 34, 46.6 %). The highest maximum concentration was observed for hexachlorobenzene (2748.03 ng/g), followed by aldrin (2313.45 ng/g) and fenitrothion (2081.13 ng/g). Pollutant concentrations did not differ between cats and dogs, sexes, and ages (p > 0.05). This study highlights the significant threat that urban areas pose to pets, and therefore, POPs should be monitored periodically in hair and other samples. To the best of our knowledge, this is the first report to investigate POP levels in hair samples from cats and dogs in Turkiye.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140890625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-08DOI: 10.1016/j.scitotenv.2024.173092
J Pawlowski, K Cermakova, T Cordier, F Frontalini, L Apothéloz-Perret-Gentil, T Merzi
Environmental DNA metabarcoding is gaining momentum as a time and cost-effective tool for biomonitoring and environmental impact assessment. Yet, its use as a replacement for the conventional marine benthic monitoring based on morphological analysis of macrofauna is still challenging. Here we propose to study the meiofauna, which is much better represented in sediment DNA samples. We focus on nematodes, which are the most numerous and diverse group of meiofauna. Our aim is to assess the potential of nematode metabarcoding to monitor impacts associated with offshore oil platform activities. To achieve this goal, we used nematode-optimized marker (18S V1V2-Nema) and universal eukaryotic marker (18S V9) region to analyse 252 sediment DNA samples collected near three offshore oil platforms in the North Sea. For both markers, we analysed changes in alpha and beta diversity in relation to distance from the platforms and environmental variables. We also defined three impact classes based on selected environmental variables that are associated with oil extraction activities and used random forest classifiers to compare the predictive performance of both datasets. Our results show that alpha- and beta-diversity of nematodes varies with the increasing distance from the platforms. The variables directly related to platform activity, such as Ba and THC, strongly influence the nematode community. The nematode metabarcoding data provide more robust predictive models than eukaryotic data. Furthermore, the nematode community appears more stable in time and space, as illustrated by the overlap of nematode datasets obtained from the same platform three years apart. A significative negative correlation between distance and Shannon diversity also advocates for higher performance of the V1V2-Nema over the V9. Overall, these results suggest that the sensitivity of nematodes is higher compared to the eukaryotic community. Hence, nematode metabarcoding has the potential to become an effective tool for benthic monitoring in marine environment.
环境 DNA 元条码作为一种省时、经济的生物监测和环境影响评估工具,正日益受到重视。然而,用它来替代传统的基于大型底栖生物形态分析的海洋底栖生物监测方法仍然具有挑战性。在此,我们建议研究小型底栖生物,它们在沉积物 DNA 样本中的代表性要好得多。我们的研究重点是线虫,它们是数量最多、种类最丰富的小型底栖生物。我们的目的是评估线虫代谢编码在监测近海石油平台活动相关影响方面的潜力。为了实现这一目标,我们使用线虫优化标记(18S V1V2-Nema)和通用真核标记(18S V9)区域分析了在北海三个近海石油平台附近采集的 252 份沉积物 DNA 样本。对于这两个标记,我们分析了α和β多样性的变化与平台距离和环境变量的关系。我们还根据与石油开采活动相关的选定环境变量定义了三个影响等级,并使用随机森林分类器比较了两个数据集的预测性能。我们的结果表明,线虫的α-和β-多样性随着与平台距离的增加而变化。与平台活动直接相关的变量,如 Ba 和 THC,对线虫群落有很大影响。与真核生物数据相比,线虫代谢编码数据能提供更可靠的预测模型。此外,线虫群落在时间和空间上似乎更加稳定,从同一平台上获得的线虫数据集相隔三年后出现重叠就说明了这一点。距离与香农多样性之间的显著负相关关系也表明 V1V2-Nema 比 V9 性能更高。总之,这些结果表明,与真核生物群落相比,线虫的灵敏度更高。因此,线虫代谢编码有可能成为海洋环境底栖生物监测的有效工具。
{"title":"Assessing the potential of nematode metabarcoding for benthic monitoring of offshore oil platforms.","authors":"J Pawlowski, K Cermakova, T Cordier, F Frontalini, L Apothéloz-Perret-Gentil, T Merzi","doi":"10.1016/j.scitotenv.2024.173092","DOIUrl":"10.1016/j.scitotenv.2024.173092","url":null,"abstract":"<p><p>Environmental DNA metabarcoding is gaining momentum as a time and cost-effective tool for biomonitoring and environmental impact assessment. Yet, its use as a replacement for the conventional marine benthic monitoring based on morphological analysis of macrofauna is still challenging. Here we propose to study the meiofauna, which is much better represented in sediment DNA samples. We focus on nematodes, which are the most numerous and diverse group of meiofauna. Our aim is to assess the potential of nematode metabarcoding to monitor impacts associated with offshore oil platform activities. To achieve this goal, we used nematode-optimized marker (18S V1V2-Nema) and universal eukaryotic marker (18S V9) region to analyse 252 sediment DNA samples collected near three offshore oil platforms in the North Sea. For both markers, we analysed changes in alpha and beta diversity in relation to distance from the platforms and environmental variables. We also defined three impact classes based on selected environmental variables that are associated with oil extraction activities and used random forest classifiers to compare the predictive performance of both datasets. Our results show that alpha- and beta-diversity of nematodes varies with the increasing distance from the platforms. The variables directly related to platform activity, such as Ba and THC, strongly influence the nematode community. The nematode metabarcoding data provide more robust predictive models than eukaryotic data. Furthermore, the nematode community appears more stable in time and space, as illustrated by the overlap of nematode datasets obtained from the same platform three years apart. A significative negative correlation between distance and Shannon diversity also advocates for higher performance of the V1V2-Nema over the V9. Overall, these results suggest that the sensitivity of nematodes is higher compared to the eukaryotic community. Hence, nematode metabarcoding has the potential to become an effective tool for benthic monitoring in marine environment.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140903635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-11DOI: 10.1016/j.scitotenv.2024.173160
Xiaomai Yuan, Xiaoyan Gu, Run Liang, Guichen Ban, Li Ma, Tieguang He, Ziting Wang
Recently, biochar and N fertilizers have been used to tackle low N use efficiency (NUE) in crops across diverse environmental conditions. The coupling of biochar and N fertilizer may impact crop N utilization through different pathways in various soil types. However, there is currently a lack of comprehensive assessment of how coupling effects specifically influence N utilization in paddy and upland crops. We conducted a meta-analysis of 175 peer-reviewed studies to assess the responses of soil properties and crop traits in paddy and upland fields under coupling effects. The results indicate that NUE (+26.1 %) and N uptake (+15.0 %) in paddy fields increase more than in upland fields (+23.7 % and +8.0 %, respectively), with the coupling effect providing NH4+ predominantly for rice and NO3- for upland crops. NH4+ increases in paddy fields (+6.9 %) but decreases in upland fields (-0.7 %), while microbial biomass carbon (MBC) decreases in paddy fields (-2.9 %) and increases in upland fields (+36.0 %). These findings suggest that coupling effects supply soil inorganic nutrients in paddies and affect microbes in uplands, thereby positively affecting crop N utilization. Specifically, the greatest increase in paddy crop yield and N use efficiency occurs when the ratio of N fertilizer to biochar exceeds 1.5 %, and in uplands, it manifests when applying 10-20 t·ha-1 of biochar and <150 kg·ha-1 N fertilizer. In conclusion, this meta-analysis explores the differential effects of biochar and N fertilizer coupling in different arable land types, offering novel insights into the utilization strategies of biochar in agricultural fields.
{"title":"Comparing combined application of biochar and nitrogen fertilizer in paddy and upland soils: Processes, enhancement strategies, and agricultural implications.","authors":"Xiaomai Yuan, Xiaoyan Gu, Run Liang, Guichen Ban, Li Ma, Tieguang He, Ziting Wang","doi":"10.1016/j.scitotenv.2024.173160","DOIUrl":"10.1016/j.scitotenv.2024.173160","url":null,"abstract":"<p><p>Recently, biochar and N fertilizers have been used to tackle low N use efficiency (NUE) in crops across diverse environmental conditions. The coupling of biochar and N fertilizer may impact crop N utilization through different pathways in various soil types. However, there is currently a lack of comprehensive assessment of how coupling effects specifically influence N utilization in paddy and upland crops. We conducted a meta-analysis of 175 peer-reviewed studies to assess the responses of soil properties and crop traits in paddy and upland fields under coupling effects. The results indicate that NUE (+26.1 %) and N uptake (+15.0 %) in paddy fields increase more than in upland fields (+23.7 % and +8.0 %, respectively), with the coupling effect providing NH<sub>4</sub><sup>+</sup> predominantly for rice and NO<sub>3</sub><sup>-</sup> for upland crops. NH<sub>4</sub><sup>+</sup> increases in paddy fields (+6.9 %) but decreases in upland fields (-0.7 %), while microbial biomass carbon (MBC) decreases in paddy fields (-2.9 %) and increases in upland fields (+36.0 %). These findings suggest that coupling effects supply soil inorganic nutrients in paddies and affect microbes in uplands, thereby positively affecting crop N utilization. Specifically, the greatest increase in paddy crop yield and N use efficiency occurs when the ratio of N fertilizer to biochar exceeds 1.5 %, and in uplands, it manifests when applying 10-20 t·ha<sup>-1</sup> of biochar and <150 kg·ha<sup>-1</sup> N fertilizer. In conclusion, this meta-analysis explores the differential effects of biochar and N fertilizer coupling in different arable land types, offering novel insights into the utilization strategies of biochar in agricultural fields.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-15DOI: 10.1016/j.scitotenv.2024.173267
Yuyun Wang, Jun Li, Yuan Chang, Su Chang, Yanting Chen, Dan Wei, Ruoqi Li, Yi Zheng, Zitong Kang, Zhen Wu, Peizhen Chen, Yuquan Wei, Ji Li, Zhi Xu
The aim of this study was to investigate the differential metabolites and core metabolic pathways caused by fungal bioaugmentation (pH regulation and Phanerochaete chrysosporium inoculation) in secondary fermentation of composting, as well as their roles in advancing humification mechanism. Metabolomics analyses showed that inoculation strengthened the expression of carbohydrate, amino acid, and aromatic metabolites, and pH regulation resulted in the up-regulation of the phosphotransferase system and its downstream carbohydrate metabolic pathways, inhibiting Toluene degradation and driving biosynthesis of aromatic amino acids via the Shikimate pathway. Partial least squares path model suggested that lignocellulose degradation, precursors especially amino acids and their metabolism process enhanced by the regulation of pH and Phanerochaete were the main direct factors for humic acid formation in composting. This finding helps to understand the regulating mechanism of fungal bioaugmentation to improve the maturity of agricultural waste composting.
{"title":"Metabolomics analysis of advancing humification mechanism in secondary fermentation of composting by fungal bioaugmentation.","authors":"Yuyun Wang, Jun Li, Yuan Chang, Su Chang, Yanting Chen, Dan Wei, Ruoqi Li, Yi Zheng, Zitong Kang, Zhen Wu, Peizhen Chen, Yuquan Wei, Ji Li, Zhi Xu","doi":"10.1016/j.scitotenv.2024.173267","DOIUrl":"10.1016/j.scitotenv.2024.173267","url":null,"abstract":"<p><p>The aim of this study was to investigate the differential metabolites and core metabolic pathways caused by fungal bioaugmentation (pH regulation and Phanerochaete chrysosporium inoculation) in secondary fermentation of composting, as well as their roles in advancing humification mechanism. Metabolomics analyses showed that inoculation strengthened the expression of carbohydrate, amino acid, and aromatic metabolites, and pH regulation resulted in the up-regulation of the phosphotransferase system and its downstream carbohydrate metabolic pathways, inhibiting Toluene degradation and driving biosynthesis of aromatic amino acids via the Shikimate pathway. Partial least squares path model suggested that lignocellulose degradation, precursors especially amino acids and their metabolism process enhanced by the regulation of pH and Phanerochaete were the main direct factors for humic acid formation in composting. This finding helps to understand the regulating mechanism of fungal bioaugmentation to improve the maturity of agricultural waste composting.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10Epub Date: 2024-05-08DOI: 10.1016/j.scitotenv.2024.173065
Tangqing He, Wei Lin, Shuo Yang, Jiaqi Du, Bhoopander Giri, Cheng Feng, Frank S Gilliam, Fuliang Zhang, Xiaoquan Zhang, Xuelin Zhang
Arbuscular mycorrhizal fungi (AMF) increase the ability of plants to obtain nitrogen (N) from the soil, and thus can affect emissions of nitrous oxide (N2O), a long-lived potent greenhouse gas. However, the mechanisms underlying the effects of AMF on N2O emissions are still poorly understood, particularly in agroecosystems with different forms of N fertilizer inputs. Utilizing a mesocosm experiment in field, we examined the effects of AMF on N2O emissions via their influence on maize root traits and denitrifying microorganisms under ammonia and nitrate fertilizer input using 15N isotope tracer. Here we show that the presence of AMF alone or both maize roots and AMF increased maize biomass and their 15N uptake, root length, root surface area, and root volume, but led to a reduction in N2O emissions under both N input forms. Random forest model showed that root length and surface area were the most important predictors of N2O emissions. Additionally, the presence of AMF reduced the (nirK + nirS)/nosZ ratio by increasing the relative abundance of nirS-Bradyrhizobium and Rubrivivax with ammonia input, but reducing nosZ-Azospirillum, Cupriavidus and Rhodopseudomonas under both fertilizer input. Further, N2O emissions were significantly and positively correlated with the nosZ-type Azospirillum, Cupriavidus and Rhodopseudomonas, but negatively correlated with the nirS-type Bradyrhizobium and Rubrivivax. These results indicate that AMF reduce N2O emissions by increasing root length to explore N nutrients and altering the community composition of denitrifiers, suggesting that effective management of N fertilizer forms interacting with the rhizosphere microbiome may help mitigate N2O emissions under future N input scenarios.
{"title":"Arbuscular mycorrhizal fungi reduce soil N<sub>2</sub>O emissions by altering root traits and soil denitrifier community composition.","authors":"Tangqing He, Wei Lin, Shuo Yang, Jiaqi Du, Bhoopander Giri, Cheng Feng, Frank S Gilliam, Fuliang Zhang, Xiaoquan Zhang, Xuelin Zhang","doi":"10.1016/j.scitotenv.2024.173065","DOIUrl":"10.1016/j.scitotenv.2024.173065","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) increase the ability of plants to obtain nitrogen (N) from the soil, and thus can affect emissions of nitrous oxide (N<sub>2</sub>O), a long-lived potent greenhouse gas. However, the mechanisms underlying the effects of AMF on N<sub>2</sub>O emissions are still poorly understood, particularly in agroecosystems with different forms of N fertilizer inputs. Utilizing a mesocosm experiment in field, we examined the effects of AMF on N<sub>2</sub>O emissions via their influence on maize root traits and denitrifying microorganisms under ammonia and nitrate fertilizer input using <sup>15</sup>N isotope tracer. Here we show that the presence of AMF alone or both maize roots and AMF increased maize biomass and their <sup>15</sup>N uptake, root length, root surface area, and root volume, but led to a reduction in N<sub>2</sub>O emissions under both N input forms. Random forest model showed that root length and surface area were the most important predictors of N<sub>2</sub>O emissions. Additionally, the presence of AMF reduced the (nirK + nirS)/nosZ ratio by increasing the relative abundance of nirS-Bradyrhizobium and Rubrivivax with ammonia input, but reducing nosZ-Azospirillum, Cupriavidus and Rhodopseudomonas under both fertilizer input. Further, N<sub>2</sub>O emissions were significantly and positively correlated with the nosZ-type Azospirillum, Cupriavidus and Rhodopseudomonas, but negatively correlated with the nirS-type Bradyrhizobium and Rubrivivax. These results indicate that AMF reduce N<sub>2</sub>O emissions by increasing root length to explore N nutrients and altering the community composition of denitrifiers, suggesting that effective management of N fertilizer forms interacting with the rhizosphere microbiome may help mitigate N<sub>2</sub>O emissions under future N input scenarios.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":null,"pages":null},"PeriodicalIF":9.8,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}