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Green and miniaturized ultrasonic-assisted extraction using natural deep eutectic solvents to extract phenolic compounds from tea samples 利用天然深共晶溶剂从茶叶样品中提取酚类化合物的绿色微型超声波辅助萃取技术
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-09-12 DOI: 10.1016/j.sampre.2024.100128
Laura Carbonell-Rozas, Roberto Romero-González, Antonia Garrido Frenich

Natural deep eutectic solvents (NADES) have emerged as promising green solvents, particularly useful in sample treatment procedures. For the first time, different types of NADES were prepared and evaluated as potential phenolic extraction solvents from tea samples (Camellia sinensis). The NADES, composed of lactic acid:glycerol:water in a molar ratio of 1:1:3, provided the best results for the simultaneous extraction of the target phenolic compounds. This NADES was also characterized by using nuclear magnetic resonance (NMR), and the presence of nuclear Overhauser enhancement effects confirmed its supramolecular structure. The experimental conditions affecting the miniaturized ultrasonic-assisted extraction (UAE) were optimized to maximize extraction efficiency by performing a design of experiments. The proposed sample treatment was combined with ultra-high performance liquid-chromatography coupled to tandem mass spectrometry detection (UHPLC-MS/MS) to determine 21 phenolic compounds including catechin derivatives and other minor compounds. The UAE-UHPLC-MS/MS method was successfully characterized and applied to the analysis of various commercial green, black, white and red tea samples. In all cases, 18 phenolic compounds were determined with concentrations ranging from 0.3 to 29,125 mg kg−1. The proposed sample treatment is a high-throughput, easy, fast and robust alternative to conventional procedures, which reduces organic solvent consumption and costs, aligned with green sample preparation principles.

天然深共晶溶剂(NADES)是一种前景广阔的绿色溶剂,尤其适用于样品处理过程。本研究首次制备了不同类型的 NADES,并对其作为茶叶样品中潜在的酚类提取溶剂进行了评估。乳酸:甘油:水摩尔比为 1:1:3 的 NADES 在同时萃取目标酚类化合物方面效果最佳。这种 NADES 还通过核磁共振(NMR)进行了表征,核奥弗霍斯增强效应的存在证实了其超分子结构。通过实验设计优化了影响微型超声辅助萃取(UAE)的实验条件,以最大限度地提高萃取效率。建议的样品处理与超高效液相色谱-串联质谱检测(UHPLC-MS/MS)相结合,测定了 21 种酚类化合物,包括儿茶素衍生物和其他次要化合物。阿联酋-超高效液相色谱-质谱/质谱法成功地表征并应用于各种绿茶、红茶、白茶和红茶样品的分析。在所有样品中,共测定了 18 种酚类化合物,其浓度范围为 0.3 至 29 125 mg kg-1。建议的样品处理方法是一种高通量、简便、快速、稳健的替代传统方法,可减少有机溶剂的消耗,降低成本,符合绿色样品制备原则。
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引用次数: 0
Preconcentration of nanoplastics using micro-electromembrane extraction across free liquid membranes 利用跨自由液膜微电解质萃取技术预富集纳米塑料
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-01 DOI: 10.1016/j.sampre.2024.100125
Muhandiramge Ranasinghe, Michael C. Breadmore, Fernando Maya

Asymmetric micro-electromembrane extraction (µ-EME) based on a free liquid membrane has been evaluated for the preconcentration of nanoplastics. A conical unit (200 µL micropipette tip) enabled the simple and reproducible formation of the required three-phase extraction system consisting of a donor solution (150 µL sample/standard solution), free liquid membrane (FLM; 10 µL 1-pentanol), and an acceptor solution (5 µL of 5 mM phosphate buffer, pH 10.7). After µ-EME, nanoplastics transferred across the FLM into the acceptor solution were quantified using capillary zone electrophoresis with diode array detection. Enrichment factors >20 and extraction recoveries >70 % were achieved for nanoplastics concentrated at 500 V during 5 min. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) of the method using 200 nm sulphonated polystyrene particles as model nanoplastics were 6.00×10−4% (w/v) and 2.00×10−3% (w/v), respectively. Intraday (n = 6) and interday (n = 6) repeatability%RSD for 5.5 × 10−3% (w/v) nanoplastics were 8.5 % and 7.2 %, respectively. µ-EME enabled an efficient sample matrix clean-up and preconcentration of nanoplastics spiked in tea sample matrices. Nanoplastics preconcentrated through the FLM for black tea resulted in an enrichment factor of 20±3.6 (n = 3), with complete sample matrix removal of UV absorbing compounds.

对基于自由液膜的不对称微电解质膜萃取(µ-EME)进行了评估,用于纳米塑料的预浓缩。锥形装置(200 µL 微量移液器吸头)能够简单且可重复地形成所需的三相萃取系统,该系统由供体溶液(150 µL 样品/标准溶液)、自由液膜(FLM;10 µL 1-戊醇)和受体溶液(5 µL 5 mM 磷酸盐缓冲液,pH 10.7)组成。µ-EME后,采用二极管阵列检测的毛细管区带电泳法对通过FLM转移到受体溶液中的纳米塑料进行定量。纳米塑料在 500 V 的电压下浓缩 5 分钟后,富集因子为 20,萃取回收率为 70%。以 200 nm 磺化聚苯乙烯颗粒为模型纳米塑料,该方法的检出限(LOD,S/N = 3)和定量限(LOQ,S/N = 10)分别为 6.00×10-4% (w/v) 和 2.00×10-3% (w/v)。5.5 × 10-3%(w/v)纳米塑料的日内(n = 6)和日间(n = 6)重复性%RSD 分别为 8.5 % 和 7.2 %。µ-EME能够高效地净化样品基质并预浓缩茶叶样品基质中添加的纳米塑料。通过 FLM 对红茶中的纳米塑料进行预浓缩,富集因子为 20±3.6(n = 3),样品基质中的紫外线吸收化合物被完全去除。
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引用次数: 0
Electromembrane extraction of human chorionic gonadotropin – A case study on mass transfer of intact protein versus signature peptide 人绒毛膜促性腺激素的电膜提取--完整蛋白质与特征肽的质量转移案例研究
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-01 DOI: 10.1016/j.sampre.2024.100126
Torstein Kige Rye , Frederik André Hansen , Trine Grønhaug Halvorsen , Stig Pedersen-Bjergaard

For the first time, we present targeted protein detection by tryptic digestion of human chorionic gonadotropin (hCG) followed by electromembrane extraction (EME). Operational parameters were optimized, and urine and serum samples spiked with hCG underwent tryptic digestion followed by EME of the βT5 signature peptide. The liquid membrane comprised nitrophenyl octyl ether (NPOE), carvacrol, and di(2-ethyl hexyl) phosphate (DEHP) at ratios of 49:49:2 (w/w/w). Extractions were performed in a conductive vial format for 45 min at 5 V. Even from highly complex digested samples of serum and urine, the signature peptide βT5 was extracted by EME and detected by LC-MS/MS. While attempts to extract intact hCG protein were unsuccessful, the extraction of the signature peptide was efficient. The extraction recovery from undigested and digested urine was 71 % (RSD = 17 %) and 116 % (RSD = 17 %), respectively. For serum, the extraction recoveries were 11 % (RSD = 23 %) for undigested samples and 110 % (RSD = 14 %) for digested samples. This study demonstrates both the potential and challenges of EME for protein analysis. Experiments regarding EME of intact proteins provided new insights into protein phase distribution. This fundamental case study underscores the potential of EME as a sample preparation technique for the targeted determination of protein biomarkers and drugs.

我们首次提出了通过胰蛋白酶消化人绒毛膜促性腺激素(hCG),然后进行电解质萃取(EME)的靶向蛋白质检测方法。对操作参数进行了优化,对添加了 hCG 的尿液和血清样本进行了胰蛋白酶消化,然后对 βT5 标志性肽进行了 EME。液膜由硝基苯辛基醚 (NPOE)、香芹酚和磷酸二(2-乙基己基)酯 (DEHP)组成,三者的比例为 49:49:2(重量比/重量比)。萃取在导电小瓶中进行,在 5 V 电压下持续 45 分钟。即使是从高度复杂的消化血清和尿液样本中,也能通过 EME 提取出特征肽 βT5,并通过 LC-MS/MS 检测出来。虽然提取完整的 hCG 蛋白的尝试并不成功,但提取特征肽的效率很高。未消化尿液和消化尿液的提取回收率分别为 71%(RSD = 17%)和 116%(RSD = 17%)。对于血清,未消化样本的提取回收率为 11%(RSD = 23%),消化样本的提取回收率为 110%(RSD = 14%)。这项研究证明了 EME 在蛋白质分析中的潜力和挑战。完整蛋白质的 EME 实验为了解蛋白质的相分布提供了新的视角。这项基础性案例研究强调了 EME 作为一种样品制备技术在有针对性地测定蛋白质生物标记物和药物方面的潜力。
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引用次数: 0
Comprehensive comparison of fatty acid methyl ester profile in different food matrices using microwave-assisted extraction and derivatization methods and comprehensive two-dimensional gas chromatography coupled with flame ionization detection 利用微波辅助萃取和衍生化方法以及二维气相色谱-火焰离子化检测法全面比较不同食品基质中脂肪酸甲酯的概况
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-08-01 DOI: 10.1016/j.sampre.2024.100124
Donatella Ferrara , Marco Beccaria , Chiara E. Cordero , Giorgia Purcaro

Analyzing fatty acids provides key insights into fat composition for industrial applications and their implications for nutrition and health. Typically, fatty acid analysis involves extracting lipids from the matrix and converting them into fatty acid methyl esters (FAME) through a derivatization process before gas chromatography (GC) analysis. Either one-step or two-step procedures can be found in the literature and as official methods. In this work, different methods exploiting microwave-assisted processes were compared with two official methods from the American Oil Chemical Society (AOCS). Especially, two types of microwave-assisted extractions were employed: solvent extraction and extraction with hydrolysis. The extracts were derivatized using either BF3 or a microwave-assisted methanolic hydrogen chloride solution. These combinations of extraction and derivatization methods were compared also with one-step microwave-assisted extraction and derivatization, and two AOCS reference methods, resulting in seven different methods applied to six different food matrices. The performance of the different procedures was compared based on the FAME profile obtained from the comprehensive two-dimensional GC (GC × GC)-FID analysis.

Microwave-assisted processes were shown to be effective, yielding results comparable to the official methods in both the one-step and two-step methods. Moreover, it was shown that the BF3 derivatization could be safely replaced with microwave-assisted derivatization with methanolic hydrogen chloride, providing equivalent performances while enhancing operator safety and environmental friendliness. Some discrepancies in the FAMEs profile were highlighted for the sample of oats, the only explicitly requiring acidic hydrolysis for lipid extraction. Further studies are required to understand the reasons behind these differences and develop a suitable modified method. In conclusion, all the methods were evaluated for greenness and blueness with two specific tools: AGREEprep and BAGI.

分析脂肪酸可以深入了解工业应用中的脂肪成分及其对营养和健康的影响。通常情况下,脂肪酸分析包括从基质中提取脂类,并在气相色谱 (GC) 分析之前通过衍生化过程将其转化为脂肪酸甲酯 (FAME)。一步法或两步法均可在文献和官方方法中找到。在这项工作中,利用微波辅助工艺的不同方法与美国石油化学学会(AOCS)的两种官方方法进行了比较。特别是采用了两种微波辅助萃取法:溶剂萃取和水解萃取。萃取物使用 BF3 或微波辅助甲醇氯化氢溶液进行衍生。将这些萃取和衍生方法的组合与一步式微波辅助萃取和衍生方法以及两种 AOCS 参考方法进行了比较,得出了适用于六种不同食品基质的七种不同方法。根据二维气相色谱(GC × GC)- FID 综合分析所得到的 FAME 图谱,对不同方法的性能进行了比较。结果表明,微波辅助方法是有效的,无论是一步法还是两步法,其结果都与官方方法相当。此外,研究还表明可以用甲醇氯化氢微波辅助衍生法安全地取代 BF3 衍生法,在提高操作安全性和环境友好性的同时提供同等的性能。燕麦样品的 FAMEs 图谱存在一些差异,这是唯一明确需要酸性水解提取脂质的样品。需要进一步研究以了解这些差异背后的原因,并开发出合适的改进方法。总之,所有方法都使用两种特定工具对绿色度和蓝色度进行了评估:AGREEprep 和 BAGI。
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引用次数: 0
Vacuum-assisted headspace-solid phase microextraction of pesticides in grape samples 葡萄样品中农药的真空辅助顶空固相微萃取
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-07-01 DOI: 10.1016/j.sampre.2024.100123
Yerkanat Syrgabek, Mereke Alimzhanova, Saltanat Yegemova, Svetlana Batyrbekova

Vacuum-assisted headspace solid-phase microextraction (Vac-HS-SPME) could provide an alternative for extracting pesticides from grape samples. Vac-HS-SPME method is utilized to the simultaneous analysis of six pesticides from various classes, namely boscalid, quizalofop-p-methyl, oxyfluorfen, fluroxypyr-meptyl, metribuzin and epoxiconazole. In this study investigated and optimized the impact of independent variables, such as extraction temperature, extraction time, fiber coating, incubation time, salt effect, sample volume, air evacuation time, pH, desorption time with the objective of achieving lower detection limits (ranging from 0.11 to 0.61 µg mL-1) and effective analyte responses. Moreover, in this work a comparison was made between classical solid phase microextraction and vacuum-assisted solid phase microextraction for the extraction of pesticides in grape samples under the same parameters. The results clearly demonstrated that the combination of Vac-HS-SPME proved to be more appropriate and selective for the extraction of pesticides from grape samples.

真空辅助顶空固相微萃取(Vac-HS-SPME)可作为从葡萄样品中萃取农药的替代方法。本研究采用 Vac-HS-SPME 方法同时分析了 6 种不同类别的农药,分别是啶虫脒、喹禾灵、氧乐果、氟虫腈、甲基立枯磷和环唑醇。本研究调查并优化了萃取温度、萃取时间、纤维涂层、培养时间、盐效应、样品体积、排空时间、pH 值、解吸时间等自变量的影响,目的是获得更低的检测限(0.11 至 0.61 µg mL-1)和有效的分析响应。此外,这项研究还比较了传统固相微萃取和真空辅助固相微萃取在相同参数下萃取葡萄样品中农药的效果。结果清楚地表明,Vac-HS-SPME 组合被证明更适合从葡萄样品中萃取农药,且选择性更强。
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引用次数: 0
Application of fourier transform infrared (FTIR) spectroscopy in sample preparation: Material characterization and mechanism investigation 傅立叶变换红外光谱在样品制备中的应用:材料表征和机理研究
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-06-28 DOI: 10.1016/j.sampre.2024.100122
Yahui Gong, Xuerong Chen, Wei Wu

The principle of Fourier Transform Infrared (FTIR) spectroscopy is based on atoms vibration and rotation, and it has become a universal and widely used spectral methodology to detect the internal molecular structures in all kinds of fields. Numerous review articles related to the applications of FTIR spectroscopy have been published in recent years. In addition, more and more scientists are aware the significance role of sample preparation to improve our life quality in life science. However, the application of FTIR spectroscopy in sample preparation hasn't been summarized. Thus, in the current paper, the application of FTIR spectroscopy in sample preparation was reviewed with the focuses on characterization of separation materials including both solid sorbents and liquid extractants, and the extraction mechanisms involved in the separation towards different targets including inorganic molecules such as rare earth elements, organic molecules such as phenolics, pharmaceuticals and illicit-drugs, and biomacromolecules of DNA and proteins. We believe that the current paper will be interested for the researchers in food science, forensic medicine, clinical medicine, environmental analysis and analytical chemistry.

傅立叶变换红外光谱(FTIR)的原理是基于原子的振动和旋转,它已成为在各种领域检测分子内部结构的一种通用而广泛应用的光谱方法。近年来,有关傅立叶变换红外光谱应用的综述文章层出不穷。此外,在生命科学领域,越来越多的科学家意识到样品制备对提高生活质量的重要作用。然而,傅立叶变换红外光谱技术在样品制备中的应用尚未得到总结。因此,本文对傅立叶变换红外光谱在样品制备中的应用进行了综述,重点关注分离材料(包括固体吸附剂和液体萃取剂)的表征,以及针对不同目标物(包括稀土元素等无机分子、酚类等有机分子、药物和违禁药物,以及 DNA 和蛋白质等生物大分子)的分离萃取机制。我们相信,食品科学、法医学、临床医学、环境分析和分析化学领域的研究人员会对本论文感兴趣。
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引用次数: 0
Revolutionizing biomarker analysis one tip at a time—Coupling antibody to Volumetric Absorptive Microsamplers for sensitive LC-MS analysis 用一个吸头彻底改变生物标记分析--将抗体与体积吸收式微型进样器耦合,实现灵敏的 LC-MS 分析
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-06-20 DOI: 10.1016/j.sampre.2024.100121
Christina Johannsen, Léon Reubsaet, Trine Grønhaug Halvorsen

The constant pursuit of efficient and cost-effective methods for analyzing human samples drives ongoing innovation in biomedical research. Introduced in 2014, Volumetric Absorptive Microsampling (VAMS) technology ensures fixed blood volume absorption and enhanced sample uniformity. Our study aims to enhance VAMS functionality by integrating sample preparation onto the device primarily used for sample collection. This involves the adsorption of antibodies onto VAMS tips, enabling instant sample clean-up at the time of sample collection. Using human chorionic gonadotropin (hCG) as a model analyte, we evaluated the qualitative and quantitative performance of Affinity-VAMS. First, we showed that adsorbing and covalently binding monoclonal antibodies to VAMS tips results in capturing of the target analyte in comparison to unmodified VAMS. Due to the ease of preparation, we moved forward using antibody adsorption to VAMS tip and optimized the procedure. Optimization of the procedure involved fine-tuning the antibody coupling step, washing process, and determining the optimal amount of antibody required, leading to a streamlined process with significant time savings up to two days. Recovery experiments demonstrate successful capture of the target analyte by the Affinity-VAMS, while matrix effects and stability assessments indicate no negative effects from serum matrix or storage conditions. Finally, quantitative analysis shows promising performance of the Affinity-VAMS in detecting different concentrations of the target analyte in the concentration range between 7.5 - 25 ng·mL−1. The calculated correlation factor was R2 of 0.9988 and limit of detection 2.5 ng·mL−1. The precision lays within the ICH guidelines for bioanalytical method validation. While this report is focused on the proof of concept of the Affinity-VAMS, our findings demonstrate the potential for the usage of modified VAMS in remote sampling and integrated sample processing, enhancing biomarker analysis in clinical and research settings.

对高效、经济的人体样本分析方法的不断追求,推动着生物医学研究领域的持续创新。体积吸收微采样(VAMS)技术于 2014 年推出,可确保固定血容量吸收并提高样本均匀性。我们的研究旨在将样本制备集成到主要用于样本采集的设备上,从而增强 VAMS 的功能。这包括将抗体吸附到 VAMS 吸头上,以便在采集样本时立即进行样本清理。我们使用人绒毛膜促性腺激素(hCG)作为模型分析物,评估了 Affinity-VAMS 的定性和定量性能。首先,我们发现,与未经修饰的 VAMS 相比,将单克隆抗体吸附并共价结合到 VAMS 顶端可捕获目标分析物。由于制备简便,我们将抗体吸附到 VAMS 顶端,并对程序进行了优化。优化过程包括微调抗体偶联步骤、洗涤过程和确定所需的最佳抗体量,从而简化了过程,大大节省了时间,最多可节省两天。回收实验表明,Affinity-VAMS 能成功捕获目标分析物,而基质效应和稳定性评估表明,血清基质或储存条件不会产生负面影响。最后,定量分析显示,Affinity-VAMS 在检测 7.5 - 25 ng-mL-1 浓度范围内不同浓度的目标分析物方面表现良好。计算得出的相关系数 R2 为 0.9988,检测限为 2.5 ng-mL-1。精确度符合 ICH 生物分析方法验证指南的要求。虽然本报告的重点是 Affinity-VAMS 的概念验证,但我们的研究结果证明了在远程采样和综合样品处理中使用改良 VAMS 的潜力,从而加强了临床和研究环境中的生物标记分析。
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引用次数: 0
The determination of estrogens in fish samples by magnetic solid phase extraction coupled to high performance liquid chromatography-diode array detector 磁性固相萃取-高效液相色谱-二极管阵列检测器测定鱼类样品中的雌激素
IF 5.2 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-06-18 DOI: 10.1016/j.sampre.2024.100120
Bin Xue , Mingzhong Xu , Zuhai Bai , Cong Hu

In this work, a novel magnetic nanoparticle (Fe3O4@SiO2@MIL-53(Al)-NH2) constructed on the basis of metal-organic frameworks (MOFs, MIL-53(Al)-NH2) was prepared, which was characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Good preparation reproducibility was obtained with the relative standard deviations (RSDs) ranging from 6.6 to 13.1 % (n = 6). In addition, the prepared Fe3O4@SiO2@MIL-53(Al)-NH2 possesses many merits, including simple preparation, greenness, good water/solvent stability and high specific surface area. The Fe3O4@SiO2@MIL-53(Al)-NH2 was used as an adsorbent for magnetic solid phase extraction (MSPE) of estrogens in fish samples. To achieve higher extraction efficiency, several factors including extraction time, pH value, ionic strength and desorption time were explored. Compared with other methods, the developed method exhibits high extraction efficiency, fast extraction kinetics and suitable for simultaneous analysis of large number of samples. The method was successfully applied to the analysis of estrogens in (spiked) fish samples, and good recoveries were obtained.

本研究制备了一种基于金属有机框架(MOFs,MIL-53(Al)-NH2)的新型磁性纳米粒子(Fe3O4@SiO2@MIL-53(Al)-NH2),并通过傅立叶变换红外光谱(FT-IR)、透射电子显微镜(TEM)、X射线衍射(XRD)和X射线光电子能谱(XPS)对其进行了表征。制备过程具有良好的重现性,相对标准偏差(RSD)在 6.6% 到 13.1% 之间(n = 6)。此外,所制备的 Fe3O4@SiO2@MIL-53(Al)-NH2 还具有制备简单、绿色环保、良好的水/溶剂稳定性和高比表面积等诸多优点。本研究以Fe3O4@SiO2@MIL-53(Al)-NH2为吸附剂,对鱼类样品中的雌激素进行了磁性固相萃取(MSPE)。为了提高萃取效率,对萃取时间、pH值、离子强度和解吸时间等因素进行了探讨。与其他方法相比,该方法萃取效率高,萃取动力学过程快,适用于大量样品的同时分析。该方法成功地应用于鱼类样品中雌激素的分析,并获得了良好的回收率。
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引用次数: 0
Pipette tip-electrospray mass spectrometry for determining opioids in urine, from on-site micro-handling to high-throughput centrifugal microextraction 从现场微处理到高通量离心微萃取,用于测定尿液中阿片类物质的移液器吸头-电喷雾质谱法
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-31 DOI: 10.1016/j.sampre.2024.100118
Jaime Millán-Santiago, Rafael Lucena, Soledad Cárdenas

Pipette tip-electrospray is a useful technique that synergically combines sample preparation and ambient ionization mass spectrometry for the rapid analysis of samples. In this work, the development of pipette tip extraction (PTE) is evaluated under two workflows that can be adapted to different analytical scenarios. Micro-handling PTE (MH-PTE) is an inherently portable technique that can be on-site applied. Centrifugal PTE (C-PTE), firstly presented here, allows a high sample throughput just requiring a benchtop centrifuge. The combination of both MH-PTE and C-PTE with pipette tip-electrospray is evaluated in this work for determining codeine and morphine, two metabolically related drugs, in urine and applied to the analysis of real samples. In the case of MH-PTE, intended to be on-site applied, the chemical stability of the analytes once extracted in the tips plays a crucial role. This stability has been studied in detail, simulating several transport/storage conditions compatible with regular postal services. The analytical features of both combinations are similar, although C-PTE provides a better sensitivity in terms of limit of detection (0.3 µg L−1 for codeine and 0.6 µg L−1 for morphine) compared to MH-PTE (0.6 µg L−1 for codeine and 1.5 µg L−1 for morphine). Moreover, the sample throughput provided by C-PTE is higher (up to 72 samples h−1) than that provided by MH-PTE (up to 12 samples h−1).

移液器吸头电喷雾技术是一种有用的技术,它将样品制备和环境电离质谱技术协同结合起来,实现了样品的快速分析。在这项工作中,根据两种工作流程对移液器吸头萃取(PTE)的发展进行了评估,这两种工作流程可适用于不同的分析场景。微处理 PTE(MH-PTE)是一种可现场应用的便携式技术。本文首次介绍的离心 PTE(C-PTE)只需一台台式离心机即可实现高样品通量。本研究评估了 MH-PTE 和 C-PTE 与吸头电喷雾技术的结合,用于测定尿液中的可待因和吗啡这两种代谢相关的药物,并将其应用于实际样品的分析。MH-PTE 用于现场应用,分析物在吸头中提取后的化学稳定性起着至关重要的作用。我们对这种稳定性进行了详细研究,模拟了与常规邮政服务相适应的几种运输/储存条件。两种组合的分析特性相似,但就检测限而言,C-PTE 的灵敏度(可待因为 0.3 µg L-1 ,吗啡为 0.6 µg L-1)高于 MH-PTE(可待因为 0.6 µg L-1 ,吗啡为 1.5 µg L-1)。此外,C-PTE 提供的样本吞吐量(最多 72 个样本/小时-1)比 MH-PTE 提供的样本吞吐量(最多 12 个样本/小时-1)高。
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引用次数: 0
Electric field-assisted dried blood spot sample preparation for analysis of steroids using LC–MS/MS 使用 LC-MS/MS 分析类固醇的电场辅助干血样制备方法
Q1 CHEMISTRY, ANALYTICAL Pub Date : 2024-05-01 DOI: 10.1016/j.sampre.2024.100115
Ramisa Fariha, Emma Rothkopf, John Murphy, Nondi Walters, Oluwanifemi David Okoh, Nabil M. Lawandy, Anubhav Tripathi

Despite being a minimally invasive sample source, dried blood spots (DBS) generally pose the challenge of efficient sample extraction for broad metabolomics applications. This is particularly true for the quantification of steroids using non-derivatized liquid chromatography tandem mass spectrometry assays. To address these limitations, we have demonstrated the use of electric field as a driver for sample preparation from DBS samples to simultaneously quantify testosterone (T), 17α-hydroxyprogesterone (17-OHP), progesterone (P), and cortisol (C), using both standard electroporation cuvettes, as well as a novel custom vertical electric field setup. Our findings, backed by computational modeling, show that a 10V DC application for 180 s can draw out twice the amount of the aforementioned steroids from both Whatman-903 and DMPK-C sample collection cards using our novel device when compared to standard solvent-based collection methods. This study not only introduces the use of electric field for sample preparation for metabolomics, but additionally introduces a novel device that eliminates the electric double layer effect in the process.

尽管干血斑(DBS)是一种微创样本来源,但在广泛的代谢组学应用中,如何高效提取样本通常是一个难题。对于使用非衍生液相色谱串联质谱测定法定量分析类固醇类药物来说尤其如此。为了解决这些局限性,我们使用标准电穿孔比色皿和新型定制垂直电场装置,演示了如何利用电场驱动 DBS 样品的样品制备,以同时量化睾酮 (T)、17α-羟基孕酮 (17-OHP)、孕酮 (P) 和皮质醇 (C)。计算模型支持的研究结果表明,与基于溶剂的标准收集方法相比,使用我们的新型装置,在 180 秒内施加 10V 直流电,从 Whatman-903 和 DMPK-C 样品收集卡中提取的上述类固醇的量是后者的两倍。这项研究不仅介绍了利用电场制备代谢组学样品的方法,而且还介绍了一种新型装置,可在此过程中消除电双层效应。
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引用次数: 0
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Advances in Sample Preparation
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