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Method: A standardised laboratory method for measuring ammonia volatilisation from pig slurry using a dynamic flux multichamber system 方法:使用动态通量多室系统测量猪浆氨挥发的标准化实验室方法
Pub Date : 2025-10-23 DOI: 10.1016/j.anopes.2025.100111
A. Antonacci , G. Lazzari , G. Bee
Ammonia (NH3) emissions generated by animal manure represent a challenge for the current livestock system, especially in the context of pig production. Standardised methods for measuring NH3 concentration in pig slurry are needed to assess whether specific management strategies (e.g. low-protein diets and feed additives) can reduce NH3 emissions. Therefore, the aim of this study was to provide a standardised procedure and test the repeatability in measuring NH3 concentration in pig slurry using a laboratory-controlled method based on a dynamic flux multichamber system. Five slurry mixes of 348 g each were prepared using spot faecal and urine samples from a single pig. Every mix was composed of 87 g of faeces, 87 g of urine, and 174 g of distilled water. For each mix, three replicates of 100 g were realised, for a total of 15 slurry replicates of five slurry mixes. The 15 slurry replicates were contained in jars, placed in a water bath (21 °C), and injected with an even flow of synthetic air (approx. 0.3 L/min). The replicates were connected to a cavity ring-down spectrometer (CRDS), which, combined with a multichannel sampler, measured the NH3 concentration of the outgoing air flow from the slurry replicates for 168.75 consecutive hours. Each slurry replicate was measured cyclically for 15 min, with a 10-min interval between two slurry replicates over 168.75 h of measurements. The values recorded during the final 60 s of each 15-min period were interpolated on an hourly basis, obtaining one NH3 concentration value per hour. To test the repeatability of the method, CVs of area under the curve for NH3 concentration (AUC NH3, ppm × h), NH3 concentration peak value (PV NH3, ppm) and time to reach the peak (TTP, h) were calculated within pairs of replicates from the same mix and within pairs of mixes. For the slurry mixes, all the CVs calculated showed a variation lower than 10%. Among the replicates, only the CV related to the TTP registered a value higher than 10% for four pairs of replicates out of 15. These findings suggest that the proposed dynamic flux multichamber system provides a standardised and repeatable approach for measuring NH3 concentration in pig slurry under controlled laboratory conditions.
动物粪便产生的氨(NH3)排放对当前的畜牧业系统构成了挑战,特别是在养猪生产的背景下。需要标准化的方法来测量猪浆中NH3的浓度,以评估特定的管理策略(如低蛋白饲粮和饲料添加剂)是否可以减少NH3的排放。因此,本研究的目的是提供一个标准化的程序,并测试使用基于动态通量多室系统的实验室控制方法测量猪浆中NH3浓度的可重复性。用一头猪的粪便和尿液样品制备了5种348克的浆液混合物。每种混合物由87克粪便、87克尿液和174克蒸馏水组成。每种料浆配制3个100 g的重复,5种料浆配制共15个料浆重复。将15个浆液副本装入罐中,置于水浴(21°C)中,并注入均匀流动的合成空气(约为100℃)。0.3升/分钟)。将重复液连接到空腔衰落光谱仪(CRDS),该光谱仪与多通道采样器结合,连续168.75小时测量浆液重复液流出气流中的NH3浓度。每个浆液重复循环测量15分钟,在168.75小时的测量中,两次浆液重复之间间隔10分钟。将每15分钟最后60秒的记录值按小时插值,得到每小时一个NH3浓度值。为了验证该方法的重复性,计算了同一混合物对内重复和对内重复NH3浓度曲线下面积(AUC NH3, ppm × h)、NH3浓度峰值(PV NH3, ppm)和达峰时间(TTP, h)的cv值。对于料浆混合料,计算得到的cv变化均小于10%。15对重复中,只有4对重复中与TTP相关的CV值大于10%。这些发现表明,所提出的动态通量多室系统为在受控的实验室条件下测量猪浆中NH3浓度提供了一种标准化和可重复的方法。
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引用次数: 0
Method: Effect of light on microbial fermentation in an in vitro system using rumen fluid from lactating dairy cows 方法:在泌乳奶牛瘤胃液体外发酵系统中,光照对微生物发酵的影响
Pub Date : 2025-09-18 DOI: 10.1016/j.anopes.2025.100110
J.R. Vinyard , R.R. Lobo , E. Sarmikasoglou , L.F. Roesch , Z. Yuting , K.C. Jeong , A.P. Faciola
Most in vitro assays used to evaluate ruminal fermentation are carried out using clear glass vessels that allow light penetration, unlike the rumen. Some bacterial species are light-sensitive, and any light stimuli may cause chemical reactions within the microbial cell that impact their survival, growth, and proliferation. Thus, this study’s objective was to evaluate light’s effects on ruminal fermentation in a dual-flow continuous culture. Using four fermenters and three experimental periods, the ruminal fluid of three lactating dairy cows was collected, pooled, and incubated for 10 days. Laboratory lights (234 ± 15 lux) were turned on 24 h per day for the entire experiment duration. Two of the fermenters were covered with aluminum foil to avoid light penetration within the jar. All fermenters were fed 106 g/day split into two feedings of the same diet that had 16% CP; 35% neutral detergent fiber; 31% starch, DM basis. After 7 days of adaptation, samples were collected for 3 days of each period for analyses of ruminal fermentation, nutrient degradation, and microbial analysis. Statistical analysis was performed with the MIXED procedure of SAS and the vegan and phyloseq packages of R. Significance was declared at P ≤ 0.05 and tendencies at 0.05 < P ≤ 0.10. There was no effect of treatment on ruminal fermentation or nutrient degradation in vitro. However, light exposure decreased species richness and diversity, decreased abundances of Actinobacteria and Planctomycetes in the liquid fraction and Actinobacteria, Fibrobacteres, Planctomycetes, and Syngergistetes in the solid fraction. Light exposure increased Synergistetes in the liquid fraction and tended to increase Proteobacteria in the solid fraction. In summary, this pilot study demonstrated that light affects ruminal microbiota. Factors such as light intensity, exposure duration, and affected populations should be further investigated. Those results indicate that some ruminal microorganisms may be light-sensitive, and whenever possible, darker conditions should be practiced.
大多数用于评估瘤胃发酵的体外试验都是使用透明玻璃容器进行的,与瘤胃不同,玻璃容器允许光线穿透。有些细菌是光敏的,任何光刺激都可能在微生物细胞内引起化学反应,影响它们的生存、生长和增殖。因此,本研究的目的是评估光对双流连续培养中瘤胃发酵的影响。采用4个发酵罐和3个试验期,收集3头泌乳奶牛的瘤胃液,进行池化,孵育10 d。在整个实验期间,每天24小时打开实验室灯(234±15勒克斯)。其中两个发酵罐用铝箔覆盖,以避免光线穿透罐内。所有发酵罐投喂106 g/d,分成两组投喂相同的饲粮,CP含量为16%;35%中性洗涤纤维;31%淀粉,DM基础。适应7 d后,每期3 d采集样品进行瘤胃发酵、营养物质降解和微生物分析。采用SAS和r的vegan和phyloseq包装进行统计学分析,P≤0.05,P≤0.10。对瘤胃体外发酵和营养物质降解均无影响。然而,光照降低了物种丰富度和多样性,降低了液体部分放线菌属和植物菌属的丰度,降低了固体部分放线菌属、纤维菌属、植物菌属和合成菌属的丰度。光照增加了液体部分的协同菌属,并倾向于增加固体部分的变形菌属。总之,这项初步研究表明,光影响瘤胃微生物群。光照强度、暴露时间和受影响人群等因素应进一步调查。这些结果表明,一些瘤胃微生物可能对光敏感,只要可能,就应该在较暗的条件下进行试验。
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引用次数: 0
Method: open-source economic tool for dairy farmers planning extended cow-calf contact 方法:为计划扩大奶牛与小牛接触的奶农提供开源经济工具
Pub Date : 2025-09-17 DOI: 10.1016/j.anopes.2025.100109
A.H. Stygar , M. Mughal , L. Frondelius , M. Pastell
Extended cow-calf contact (CCC) is gaining popularity among European dairy farmers. Any change in farm management strategy should be preceded by economic considerations and an accurate assessment of the costs and benefits of the new management strategy. In this study, we have developed an economic tool for farmers planning extended cow-calf contact. The tool allows producers to calculate the costs and benefits of the new practice (CCC lasting from 1 to 3 months) and compare it with the current operation (conventional system with a maximum of 1 day of cow-calf contact). The tool has been developed using the free and open source R package Shiny and is accessible from the following link: https://cowcalfeconomictool.github.io/. The CowCalfEconomicTool consists of two tabs: calculator and user guide. The calculator tab has 7 distinct frames: language selection, housing, herd size, operational costs and revenues, milk production, calf parameters in the current system and expected CCC effect. Additionally, the calculator tab has one output frame which is dedicated to presenting an interactive cost-benefit table, a Gauge cost-benefit chart, net present value and a cost-benefit ratio. Finally, the user can download all input and output data. To test the economic model behaviour and determine which parameter has the biggest impact on the economic result, sensitivity analyses were conducted. Tool verification was performed with a group of experts. The source code for the tool as well as baseline variables and results of sensitivity analysis are available from the GitHub repository: https://github.com/CowCalfEconomicTool/CowCalfApp.
Sensitivity analyses indicated that building costs together with additional milk price premium for improved welfare were the most critical factors affecting the net present value of CCC operations. The user is encouraged to use the tool as a simulator by adjusting production variables accordingly. CowCalfEconomicTool is still in early stages of the development. As CCC research progresses, the default values should be adjusted and new fields could be added in the calculations. The tool assists farmers to make informed decisions on whether and how to adopt CCC, but the results of the analyses have to be used with caution. The final decision should be made after careful consideration and consultation with relevant experts (e.g. farm advisors, barn planning companies, veterinarians).
长时间接触母牛(CCC)在欧洲奶农中越来越受欢迎。在改变农场管理战略之前,应先考虑经济因素,并对新管理战略的成本和效益进行准确评估。在这项研究中,我们开发了一种经济工具,供农民计划扩大牛与小牛的接触。该工具允许生产者计算新做法(持续1至3个月的CCC)的成本和效益,并将其与当前操作(传统系统,最多1天的牛-小牛接触)进行比较。该工具是使用免费的开源R包Shiny开发的,可以从以下链接访问:https://cowcalfeconomictool.github.io/。CowCalfEconomicTool包含两个选项卡:计算器和用户指南。计算器标签有7个不同的框架:语言选择、住房、畜群规模、运营成本和收入、产奶量、当前系统中的小牛参数和预期的CCC效果。此外,计算器选项卡有一个输出框架,用于显示交互式成本效益表、Gauge成本效益图表、净现值和成本效益比。最后,用户可以下载所有输入和输出数据。为了检验经济模型的行为,确定哪个参数对经济结果的影响最大,进行了敏感性分析。由一组专家进行工具验证。该工具的源代码以及基线变量和敏感性分析的结果可从GitHub存储库获得:https://github.com/CowCalfEconomicTool/CowCalfApp.Sensitivity分析表明,建筑成本以及为改善福利而增加的牛奶价格溢价是影响CCC运营净现值的最关键因素。鼓励用户通过相应地调整生产变量来使用该工具作为模拟器。CowCalfEconomicTool仍处于开发的早期阶段。随着CCC研究的深入,需要调整默认值,并在计算中增加新的字段。该工具帮助农民就是否以及如何采用CCC做出明智的决定,但必须谨慎使用分析结果。最终决定应在仔细考虑并咨询相关专家(如农场顾问、畜棚规划公司、兽医)后做出。
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引用次数: 0
Method: An accurate method for detecting drinking bouts in dairy cows based on reticulorumen temperature 方法:建立基于网腔温度的奶牛饮期检测方法
Pub Date : 2025-09-15 DOI: 10.1016/j.anopes.2025.100107
L. Aubé , K. Pelletier , B. Meunier , A. de Boyer des Roches , R. Lardy , D. Ledoux
This study evaluated the performances of three methods for detecting drinking bouts in dairy cows using reticulorumen temperature (RT): the ‘FixT’ method based on a fixed RT threshold, the ‘Cow-dT’ method based on a cow-day-specific RT threshold, and the ‘FallST’ method based on RT fall slope. We observed the drinking behaviours of 28 dairy cows equipped with reticulorumenal sensors over 96 h to create a reference dataset. A total of 730 drinking bouts were observed. We matched detected drinking bouts against observed drinking bouts to obtain the number of true-positives, false-negatives, and false-positives, and then calculated the detection performances of the three methods in terms of sensitivity (Se), positive predictive value (PPV), and F-score. The performances of the three RT-based methods (Se ≥ 90%, PPV > 96% and F-score ≥ 93%) were better than those from previous work using collar-attached accelerometers, but slightly lower than methods using drinking troughs connected to electronic identification systems or methods combining accelerometers with geomagnetic sensors or with ultra-wideband location. The FallST method showed slightly better performance (highest F-score) than the FixT and Cow-dT methods. The FallST method accurately detected drinking bouts lasting more than 30 s and at least 30 min apart, with a detection time accuracy of 10 min. The models using RT curve parameters failed to predict characteristics of the drinking bouts. In conclusion, the method developed here can accurately detect drinking bouts in dairy cows using RT, but without further characterisation of the drinking bouts (e.g. duration).
本研究评估了三种利用网口温度(RT)检测奶牛饮水量的方法的性能:基于固定RT阈值的“FixT”方法、基于奶牛日特异性RT阈值的“Cow-dT”方法和基于RT下降斜率的“FallST”方法。我们观察了28头配有网状尿路传感器的奶牛在96小时内的饮酒行为,以创建参考数据集。共观察了730例饮酒事件。我们将检测到的饮酒次数与观察到的饮酒次数进行匹配,得到真阳性、假阴性和假阳性的数量,然后计算三种方法在灵敏度(Se)、阳性预测值(PPV)和f分数方面的检测性能。三种基于rt的方法(Se≥90%,PPV > 96%, f评分≥93%)的性能优于先前使用项圈附加加速度计的方法,但略低于使用连接电子识别系统的饮水槽或将加速度计与地磁传感器或超宽带定位相结合的方法。与FixT和Cow-dT方法相比,FallST方法的表现稍好(f值最高)。FallST方法可准确检测持续时间超过30秒且间隔时间至少为30分钟的饮酒发作,检测时间精度为10分钟。使用RT曲线参数的模型无法预测饮酒的特征。总之,本文开发的方法可以使用RT准确地检测奶牛的饮酒发作,但没有进一步的饮酒发作特征(例如持续时间)。
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引用次数: 0
Cartilage volume and body composition in fattening pigs in different housing systems using magnetic resonance imaging and dual-energy X-ray absorptiometry 利用磁共振成像和双能x线吸收仪研究不同饲养系统育肥猪的软骨体积和体成分
Pub Date : 2025-09-15 DOI: 10.1016/j.anopes.2025.100108
T. Dölle , M. Bernau , A.M. Scholz
Lameness and diseases of the cartilage and skeletal system in pigs are an important animal welfare issue in connection with the goal of high profitability. The aim of the study is therefore to investigate the influence of the housing system on the expression of cartilage tissue in relation to the body composition of fattening pigs. Magnetic resonance (MR) imaging was combined with dual−energy X-ray absorptiometry (DXA) to determine cartilage and body tissue characteristics. A total of 127 crossbred pigs (Piétrain × German Landrace) were examined in 3 different fattening rounds. After the first test at a live weight of 30 kg, half of the animals in each round were randomly assigned to a fully slatted housing system (multi-purpose barn = MPB) or an outdoor climate barn (OCB) with deep litter. In this step, the aim was to achieve a balanced sex ratio of female (n = 62) and male castrated pigs (n = 65). The animals remained in the respective housing system until the end of the fattening period. Each pig was examined three times during the fattening period: at 25–30 kg (scan 30), at 60–70 kg (scan 60) and at 90–100 kg (scan 90) BW. During the MR imaging examination with a Siemens Magnetom Open, two T1-weighted sequences were created of each pig at each examination time point: a sagittal sequence of the left elbow and shoulder joint and a coronary sequence of the left and right knee joint. Subsequently, a whole-body scan was performed on each pig using the GE Lunar iDXA scanner and the following values were automatically determined: bone mineral density [g/cm2], absolute bone mineral content [g] (BMC), bone mineral area projection [cm2], fat tissue percentage [%] (Fat_PC), fat tissue [g], and soft lean tissue [g]. In the knee joint, there were no significant differences in cartilage volume (CVo) in relation to the housing system. In the elbow joints, however, there were significant differences in CVo at the time of scan 30 that could not be explained. In contrast, the results of the DXA study show that pigs in the OCB have significantly higher BMC values than pigs from the MPB from scan 60 onwards. These differences indicate that the housing system has an effect on bone mineralisation, but possibly not on the CVo during pig fattening.
猪软骨和骨骼系统的跛行和疾病是与高盈利目标相关的重要动物福利问题。因此,本研究的目的是研究饲养系统对育肥猪体组成中软骨组织表达的影响。磁共振(MR)成像结合双能x线吸收仪(DXA)来确定软骨和身体组织特征。采用3个不同育肥轮,对127头杂交猪(皮姆-德长)进行育肥试验。在活重为30公斤的第一次试验后,每轮中有一半的动物被随机分配到全板条住房系统(多用途谷仓= MPB)或室外气候谷仓(OCB)。在这一步中,目标是实现雌性(n = 62)和雄性阉割猪(n = 65)的平衡性别比例。在育肥期结束之前,这些动物一直呆在各自的饲养系统中。在育肥期,每头猪分别在体重25-30 kg(扫描30)、60 - 70 kg(扫描60)和90 - 100 kg(扫描90)时接受三次检查。在使用Siemens Magnetom Open进行MR成像检查时,在每个检查时间点对每只猪创建两个t1加权序列:左肘关节和肩关节的矢状序列和左、右膝关节的冠状序列。随后,使用GE Lunar iDXA扫描仪对每头猪进行全身扫描,并自动确定以下数值:骨矿物质密度[g/cm2]、绝对骨矿物质含量[g] (BMC)、骨矿物质面积投影[cm2]、脂肪组织百分比[%](Fat_PC)、脂肪组织[g]和软瘦肉组织[g]。在膝关节中,软骨体积(CVo)与住房系统没有显著差异。然而,在肘关节中,扫描时CVo有显著差异30,这无法解释。相比之下,DXA研究结果显示,从扫描60开始,OCB中的猪的BMC值明显高于MPB中的猪。这些差异表明,猪舍系统对骨矿化有影响,但可能对猪育肥期间的CVo没有影响。
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引用次数: 0
Method: Comparing averaging methods for gas flux data generated by automated head chamber systems 方法:比较自动化头室系统产生的气体通量数据的平均方法
Pub Date : 2025-09-08 DOI: 10.1016/j.anopes.2025.100106
M.R. Beck , L.R. Thompson , C.A. Moffet , R.R. Reuter , S.A. Gunter
Researchers are increasingly using automated head chamber systems (GreenFeed; C-Lock inc., Rapid City, SD) for estimating gaseous emissions, such as carbon dioxide and methane, and consumption, such as oxygen. Our objective was to explore different data preprocessing methods. For this investigation, we collated data from 5 previously published manuscripts – 3 from grazing studies and 2 from studies utilizing finishing beef steers. We compared simple arithmetic or time-bin (8, 3-h intervals) averaging and least-squares means (LSMEANS) methodologies to arrive at a single estimate for each animal from gas estimates for each visit. For the LSMEANS approach, a mixed effects model was fit for each gas as the dependent variable, animal ID as fixed effects, visit duration and average airflow as covariates, and date and hour of day by animal ID as random effects. If duration and average airflow were not significant, they were removed from the model. After fitting the model, LSMEANS were generated for each animal with a standard error of the mean for each animal estimate. We then analyzed the data for each experiment according to the model presented in its respective manuscript, to obtain residual standard deviation and to calculate the coefficient of variation. Time-bin averaging increased unexplained error relative to arithmetic averaging and the LSMEANS approach. The increased unexplained error resulted in time-bin averaging having a greater coefficient of variation by 11.2% for pasture and 6.1% for finishing trials compared with arithmetic averaging and by 13.5% for pasture and 6.1% for finishing trials compared with the LSMEANS approach. We conclude that the proposed LSMEANS approach controls for any potential diurnal variation in gas flux, without increasing unexplained error as seen by time-bin averaging.
研究人员越来越多地使用自动化头室系统(GreenFeed; C-Lock inc., Rapid City, SD)来估计气体排放,如二氧化碳和甲烷,以及消耗,如氧气。我们的目标是探索不同的数据预处理方法。在这项调查中,我们整理了先前发表的5篇论文的数据,其中3篇来自放牧研究,2篇来自育肥牛研究。我们比较了简单的算术或时间桶(8,3小时间隔)平均和最小二乘平均(LSMEANS)方法,从每次访问的气体估计中得出每只动物的单一估计。对于LSMEANS方法,将每种气体作为因变量拟合为混合效应模型,动物ID为固定效应,访问时间和平均气流为协变量,动物ID的日期和小时为随机效应。如果持续时间和平均气流不显著,则将其从模型中删除。拟合模型后,对每只动物生成LSMEANS,每只动物估计值的标准误差为平均值。然后,我们根据各自手稿中的模型对每个实验的数据进行分析,得到残差标准差,并计算变异系数。相对于算术平均和LSMEANS方法,时间仓平均增加了无法解释的误差。与算术平均法相比,无法解释的误差增加导致时间bin平均法的变异系数在放牧试验中增加了11.2%,在育肥试验中增加了6.1%,在放牧试验中增加了13.5%,在育肥试验中增加了6.1%。我们的结论是,提出的LSMEANS方法控制了气体通量的任何潜在日变化,而不会增加时间桶平均所看到的无法解释的误差。
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引用次数: 0
Data paper: Behavioural and production data of sows fed tailored diets over three consecutive gestations 数据论文:连续三个妊娠期饲喂定制日粮的母猪的行为和生产数据
Pub Date : 2025-08-12 DOI: 10.1016/j.anopes.2025.100105
C. Ribas , A. Blanc , M. Simon , J. Thomas , N. Quiniou , C. Gaillard
This database was created to better understand the long-term impact of precision feeding (PF, i.e. delivery of an individualised mix of different diets to meet estimated individual requirements) on the performances of gestating sows, compared to sows fed a conventional feeding strategy (i.e., delivery of a single diet throughout gestation). Two experiments were carried out with feed supplies to PF sows adjusted individually and daily when they were grouped, based on energy and amino acids requirements (trial 1) and additionally on minerals during the last month of gestation (trial 2). Sows were group-housed from day 3 (trial 1) or day 25 (trial 2) to day 108 of gestation. Data were collected from sows over one to three successive gestations cumulating 58 (trial 1) or 77 (trial 2) gestations. The gestation pens were equipped with electronic feeder and water dispensers to characterise the feeding and drinking behaviours, with an automated scale to characterise the BW and with cameras to characterise the behaviour and location of sows, on a daily and individual basis. An automatic video analysis software analysed sows’ physical activity at the group scale, on three different stages of gestation. Physical activity was also recorded individually with accelerometers for a few numbers of sows. Social interactions, activities, and pen location were characterised at the individual level on specific periods from video manual analysis. Backfat thickness (BT) was measured manually with an ultrasound portable device. Cleanliness, scratches and lameness were recorded weekly during gestation. Each gestation room was equipped with devices which enable to follow ambient temperature, relative humidity, and other parameters such as noise or methane concentration. At farrowing, litter size and birthweight were measured. Data were used to analyse the effect of feeding strategies on feeding behaviour, variation of BW and BT during gestation, health status, social interactions when sows were group-housed, and litter characteristics at farrowing. The database contains a large amount of data including feed composition and intakes, behaviours, ambient parameters, and sow performances of group-housed gestating sows enabling to use unsupervised data mining approaches. Longitudinal database may be used to correlate feeding behaviour with physical activity or ambient parameters. Common phenotypes (BW, BT) collected at different key stages of gestation may be used to extend the existing dataset to have a greater statistical power.
该数据库的建立是为了更好地了解精确喂养(PF,即提供不同日粮的个性化组合,以满足估计的个体需求)对妊娠母猪性能的长期影响,与传统喂养策略(即在整个妊娠期间提供单一日粮)的母猪相比。试验进行了两个试验,分别根据能量和氨基酸需求(试验1)和妊娠最后一个月的矿物质需求(试验2),对PF母猪进行单独和分组时的每日饲料供应调整。母猪从妊娠第3天(试验1)或第25天(试验2)至第108天分组饲养。数据收集于母猪连续妊娠1 - 3次,累计妊娠58次(试验1)或77次(试验2)。妊娠栏配备了电子喂食器和饮水机,以表征喂养和饮水行为,配备了自动秤,以表征体重,并配备了摄像机,以表征母猪的行为和位置,每天和个别的基础。一个自动视频分析软件分析了母猪在妊娠三个不同阶段的身体活动情况。还用加速度计分别记录了几头母猪的身体活动。社会互动、活动和笔的位置在特定时期从视频手工分析中在个人层面上进行表征。用便携式超声仪手工测量背膘厚度(BT)。妊娠期间每周记录清洁、抓痕和跛行情况。每个妊娠室都配备了能够跟踪环境温度、相对湿度和其他参数(如噪音或甲烷浓度)的设备。分娩时,测量产仔数和出生体重。采用数据分析了不同饲养策略对母猪摄食行为、妊娠期体重和BT变化、健康状况、群养母猪社会交往和产仔特征的影响。该数据库包含大量数据,包括群养妊娠母猪的饲料组成和采食量、行为、环境参数和母猪性能,可以使用无监督数据挖掘方法。纵向数据库可用于将摄食行为与身体活动或环境参数联系起来。在妊娠不同关键阶段收集的共同表型(BW, BT)可用于扩展现有数据集,使其具有更大的统计能力。
{"title":"Data paper: Behavioural and production data of sows fed tailored diets over three consecutive gestations","authors":"C. Ribas ,&nbsp;A. Blanc ,&nbsp;M. Simon ,&nbsp;J. Thomas ,&nbsp;N. Quiniou ,&nbsp;C. Gaillard","doi":"10.1016/j.anopes.2025.100105","DOIUrl":"10.1016/j.anopes.2025.100105","url":null,"abstract":"<div><div>This database was created to better understand the long-term impact of precision feeding (<strong>PF</strong>, i.e. delivery of an individualised mix of different diets to meet estimated individual requirements) on the performances of gestating sows, compared to sows fed a conventional feeding strategy (i.e., delivery of a single diet throughout gestation). Two experiments were carried out with feed supplies to PF sows adjusted individually and daily when they were grouped, based on energy and amino acids requirements (trial 1) and additionally on minerals during the last month of gestation (trial 2). Sows were group-housed from day 3 (trial 1) or day 25 (trial 2) to day 108 of gestation. Data were collected from sows over one to three successive gestations cumulating 58 (trial 1) or 77 (trial 2) gestations. The gestation pens were equipped with electronic feeder and water dispensers to characterise the feeding and drinking behaviours, with an automated scale to characterise the BW and with cameras to characterise the behaviour and location of sows, on a daily and individual basis. An automatic video analysis software analysed sows’ physical activity at the group scale, on three different stages of gestation. Physical activity was also recorded individually with accelerometers for a few numbers of sows. Social interactions, activities, and pen location were characterised at the individual level on specific periods from video manual analysis. Backfat thickness (<strong>BT</strong>) was measured manually with an ultrasound portable device. Cleanliness, scratches and lameness were recorded weekly during gestation. Each gestation room was equipped with devices which enable to follow ambient temperature, relative humidity, and other parameters such as noise or methane concentration. At farrowing, litter size and birthweight were measured. Data were used to analyse the effect of feeding strategies on feeding behaviour, variation of BW and BT during gestation, health status, social interactions when sows were group-housed, and litter characteristics at farrowing. The database contains a large amount of data including feed composition and intakes, behaviours, ambient parameters, and sow performances of group-housed gestating sows enabling to use unsupervised data mining approaches. Longitudinal database may be used to correlate feeding behaviour with physical activity or ambient parameters. Common phenotypes (BW, BT) collected at different key stages of gestation may be used to extend the existing dataset to have a greater statistical power.</div></div>","PeriodicalId":100083,"journal":{"name":"Animal - Open Space","volume":"4 ","pages":"Article 100105"},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144829403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantification of the effect of reducing dietary crude protein in broiler chickens on nitrogen flows and litter characteristics by meta-analysis 采用meta分析定量研究饲粮粗蛋白质降低对肉仔鸡氮流和产仔特性的影响
Pub Date : 2025-07-02 DOI: 10.1016/j.anopes.2025.100104
T. de Rauglaudre , B. Méda , S. Fournel , M.-P. Létourneau-Montminy
In broiler chickens, reducing dietary CP content is an effective strategy to improve the efficiency of dietary nitrogen (N) utilisation by broilers while reducing N losses through volatilisation. The purpose of this study was to quantify the effect of lowering dietary CP on N flows (intake, retention, excretion, manure accumulation, and volatilisation). The database included studies that measured N volatilisation using a mass balance approach. A total of nine papers describing 16 trials and 46 observations were found. The effect of CP content on N flow variables was assessed using a linear mixed-effects model with the trial as a random effect. Broilers of the control treatments ingested an average of 4.2 g of N per day and retained an average of 55% of this N. Nitrogen intake that was not retained was excreted (1.9 g/d), and 33% of this excreted N was lost through volatilisation. Reducing dietary CP by 1% point (%-point) decreased N intake by 0.21 g/d (P < 0.001) without any effect on N retention. Nitrogen excretion and the volatility of excreted N decreased by 0.20 g/d and 4.22%-points, respectively, for each 1%-point reduction in CP content (P < 0.001). The synergy between the reduced excreted N and its volatility decreased the amount of volatilised N by 0.12 g/d (−23%) for every 1%-point reduction in CP content (P < 0.001). Reducing CP content also decreased litter mass (P < 0.05) and increased its DM (P < 0.01). The data presented show that increasing litter DM content (P < 0.05) and lowering pH (P < 0.001) reduces the volatility of excreted nitrogen. The results of this meta-analysis highlight the benefits of this low-CP diet for reducing N losses, and the equations created can be used in future evaluations of the effects of reducing dietary CP content.
降低肉仔鸡饲粮粗蛋白质含量是提高肉仔鸡饲粮氮利用效率、减少氮挥发损失的有效策略。本研究的目的是量化降低饲粮粗蛋白质对氮流(摄入、滞留、排泄、粪便积累和挥发)的影响。该数据库包括使用质量平衡方法测量N挥发的研究。共有9篇论文描述了16项试验和46项观察结果。采用线性混合效应模型评估CP含量对N流量变量的影响,试验为随机效应。对照处理肉鸡平均每天摄入4.2 g氮,平均保留55%的氮。未保留的氮被排出体外(1.9 g/d),其中33%的氮通过挥发损失。饲粮粗蛋白质降低1% (%-point),氮摄入量降低0.21 g/d (P <;0.001),对氮保留没有任何影响。CP含量每降低1个点,氮排泄量和氮挥发性分别降低0.20 g/d和4.22%;0.001)。CP含量每降低1个点(P <; P),减少的N排泄量与其挥发性之间的协同作用使N的挥发量减少0.12 g/d(- 23%)。0.001)。降低CP含量也降低了凋落物质量(P <;0.05), DM升高(P <;0.01)。数据表明,增加凋落物DM含量(P <;0.05),降低pH值(P <;0.001)降低了排出氮的挥发性。这项荟萃分析的结果强调了这种低粗蛋白质饮食对减少氮损失的好处,并且所创建的方程可用于未来评估降低饮食粗蛋白质含量的效果。
{"title":"Quantification of the effect of reducing dietary crude protein in broiler chickens on nitrogen flows and litter characteristics by meta-analysis","authors":"T. de Rauglaudre ,&nbsp;B. Méda ,&nbsp;S. Fournel ,&nbsp;M.-P. Létourneau-Montminy","doi":"10.1016/j.anopes.2025.100104","DOIUrl":"10.1016/j.anopes.2025.100104","url":null,"abstract":"<div><div>In broiler chickens, reducing dietary CP content is an effective strategy to improve the efficiency of dietary nitrogen (<strong>N</strong>) utilisation by broilers while reducing N losses through volatilisation. The purpose of this study was to quantify the effect of lowering dietary CP on N flows (intake, retention, excretion, manure accumulation, and volatilisation). The database included studies that measured N volatilisation using a mass balance approach. A total of nine papers describing 16 trials and 46 observations were found. The effect of CP content on N flow variables was assessed using a linear mixed-effects model with the trial as a random effect. Broilers of the control treatments ingested an average of 4.2 g of N per day and retained an average of 55% of this N. Nitrogen intake that was not retained was excreted (1.9 g/d), and 33% of this excreted N was lost through volatilisation. Reducing dietary CP by 1% point (%-point) decreased N intake by 0.21 g/d (<em>P</em> &lt; 0.001) without any effect on N retention. Nitrogen excretion and the volatility of excreted N decreased by 0.20 g/d and 4.22%-points, respectively, for each 1%-point reduction in CP content (<em>P</em> &lt; 0.001). The synergy between the reduced excreted N and its volatility decreased the amount of volatilised N by 0.12 g/d (−23%) for every 1%-point reduction in CP content (<em>P</em> &lt; 0.001). Reducing CP content also decreased litter mass (<em>P</em> &lt; 0.05) and increased its DM (<em>P</em> &lt; 0.01). The data presented show that increasing litter DM content (<em>P</em> &lt; 0.05) and lowering pH (<em>P</em> &lt; 0.001) reduces the volatility of excreted nitrogen. The results of this meta-analysis highlight the benefits of this low-CP diet for reducing N losses, and the equations created can be used in future evaluations of the effects of reducing dietary CP content.</div></div>","PeriodicalId":100083,"journal":{"name":"Animal - Open Space","volume":"4 ","pages":"Article 100104"},"PeriodicalIF":0.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum to “A workflow to study the microbiota profile of piglet’s umbilical cord blood: from sampling to data analysis” [Animal Open Space 2 (2023) 100031] “研究仔猪脐带血微生物群的工作流程:从采样到数据分析”[动物开放空间2(2023)100031]的勘误
Pub Date : 2025-06-04 DOI: 10.1016/j.anopes.2025.100102
Francesco Palumbo , Marion Girard , Federico Correa , Giuseppe Bee , Paolo Trevisi
{"title":"Erratum to “A workflow to study the microbiota profile of piglet’s umbilical cord blood: from sampling to data analysis” [Animal Open Space 2 (2023) 100031]","authors":"Francesco Palumbo ,&nbsp;Marion Girard ,&nbsp;Federico Correa ,&nbsp;Giuseppe Bee ,&nbsp;Paolo Trevisi","doi":"10.1016/j.anopes.2025.100102","DOIUrl":"10.1016/j.anopes.2025.100102","url":null,"abstract":"","PeriodicalId":100083,"journal":{"name":"Animal - Open Space","volume":"4 ","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum to “Method: Standard operating procedure for the administration of swallowable devices to study pig’s gut content in a non-invasive way” [Animal Open Space 3 (2024) 100076] “方法:以无创方式使用可吞咽装置研究猪肠道内容物的标准操作程序”的勘误[动物开放空间3 (2024)100076]
Pub Date : 2025-06-04 DOI: 10.1016/j.anopes.2025.100101
I. García Viñado , G. Bee , P. Trevisi , C. Ollagnier
{"title":"Erratum to “Method: Standard operating procedure for the administration of swallowable devices to study pig’s gut content in a non-invasive way” [Animal Open Space 3 (2024) 100076]","authors":"I. García Viñado ,&nbsp;G. Bee ,&nbsp;P. Trevisi ,&nbsp;C. Ollagnier","doi":"10.1016/j.anopes.2025.100101","DOIUrl":"10.1016/j.anopes.2025.100101","url":null,"abstract":"","PeriodicalId":100083,"journal":{"name":"Animal - Open Space","volume":"4 ","pages":"Article 100101"},"PeriodicalIF":0.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Animal - Open Space
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