Pub Date : 2023-11-11DOI: 10.3390/chemosensors11110562
Francisco Contini Barreto, Erika Yukie Ito, Naelle Kita Mounienguet, Letícia Dal’ Evedove Soares, Jie Yang, Quan (Sophia) He, Ivana Cesarino
This research describes the modification of a glassy carbon electrode with spent coffee grounds hydrochar (HDC) and copper nanoparticles (CuNPs) for the simultaneous determination of hydroxychloroquine sulfate (HCS) and bisphenol A (BPA). Scanning electron microscopy, EDS and cyclic voltammetry were used to characterize the nanocomposite. The analytical parameters were optimized and the sensing platform was applied for the determination of HCS and BPA using square-wave voltammetry (SWV). For HCS, the linear range was from 1.0 μmol L−1 to 50 μmol L−1, with an LOD and LOQ of 0.46 and 1.53 μmol L−1, respectively. For BPA, the linear range was from 0.5 μmol L−1 to 10 μmol L−1, with an LOD and LOQ of 0.31 μmol L−1 and 1.06 μmol L−1, respectively. Finally, the developed electrochemical sensor was applied for the quantification of the emerging contaminants in natural water, with recoveries between 94.8% and 106.8% for HCS and 99.6% and 105.2% for BPA. Therefore, HDC-CuNPs demonstrated themselves to be a good alternative as a sustainable and cheaper material for application in electroanalyses.
{"title":"Electrochemical Sensor Based on Spent Coffee Grounds Hydrochar and Metal Nanoparticles for Simultaneous Detection of Emerging Contaminants in Natural Water","authors":"Francisco Contini Barreto, Erika Yukie Ito, Naelle Kita Mounienguet, Letícia Dal’ Evedove Soares, Jie Yang, Quan (Sophia) He, Ivana Cesarino","doi":"10.3390/chemosensors11110562","DOIUrl":"https://doi.org/10.3390/chemosensors11110562","url":null,"abstract":"This research describes the modification of a glassy carbon electrode with spent coffee grounds hydrochar (HDC) and copper nanoparticles (CuNPs) for the simultaneous determination of hydroxychloroquine sulfate (HCS) and bisphenol A (BPA). Scanning electron microscopy, EDS and cyclic voltammetry were used to characterize the nanocomposite. The analytical parameters were optimized and the sensing platform was applied for the determination of HCS and BPA using square-wave voltammetry (SWV). For HCS, the linear range was from 1.0 μmol L−1 to 50 μmol L−1, with an LOD and LOQ of 0.46 and 1.53 μmol L−1, respectively. For BPA, the linear range was from 0.5 μmol L−1 to 10 μmol L−1, with an LOD and LOQ of 0.31 μmol L−1 and 1.06 μmol L−1, respectively. Finally, the developed electrochemical sensor was applied for the quantification of the emerging contaminants in natural water, with recoveries between 94.8% and 106.8% for HCS and 99.6% and 105.2% for BPA. Therefore, HDC-CuNPs demonstrated themselves to be a good alternative as a sustainable and cheaper material for application in electroanalyses.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135086482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fentanyl and its derivatives have been mainstays for the treatment of pain for many years. To accurately detect them in medical applications and customs, a rapid, sensitive, and selective method is urgently needed. In this study, we established a point-of-care-testing (POCT) differential Raman approach for the detection of fentanyl substances in liquid and solid conditions. The silver nanoparticle was prepared and characterized as SERS substrate, which can adsorb fentanyl-related molecules on the rough surface to enhance the Raman signal. Subsequently, 27 kinds of fentanyl-related substances were detected to determine that the POCT spectral resolution is better than 6 cm−1, Raman detection range is 100–3200 cm−1, and the detection limit of the fentanyl-related substances at 1002 cm−1 is 0.1–25 ppb. Furthermore, the Raman characteristic peaks of fentanyl were checked through comparison between theoretical calculations and experiments to obtain a database for rapid on-site inspection. Thus, the fast, accurate, stable POCT approach can be widely applied to monitor drugs and toxins due to its sensitivity, specificity, and abundance database.
{"title":"The Detection of 27 Fentanyl Compounds in Solid and Liquid Drugs Based on Differential Raman Spectroscopy","authors":"Yufeng Wang, Wanli Sheng, Xiang Liu, Jiajuan Guo, Xun Zhang, Xiaohua Qi, Mingqiang Zou, Cong Wang","doi":"10.3390/chemosensors11110561","DOIUrl":"https://doi.org/10.3390/chemosensors11110561","url":null,"abstract":"Fentanyl and its derivatives have been mainstays for the treatment of pain for many years. To accurately detect them in medical applications and customs, a rapid, sensitive, and selective method is urgently needed. In this study, we established a point-of-care-testing (POCT) differential Raman approach for the detection of fentanyl substances in liquid and solid conditions. The silver nanoparticle was prepared and characterized as SERS substrate, which can adsorb fentanyl-related molecules on the rough surface to enhance the Raman signal. Subsequently, 27 kinds of fentanyl-related substances were detected to determine that the POCT spectral resolution is better than 6 cm−1, Raman detection range is 100–3200 cm−1, and the detection limit of the fentanyl-related substances at 1002 cm−1 is 0.1–25 ppb. Furthermore, the Raman characteristic peaks of fentanyl were checked through comparison between theoretical calculations and experiments to obtain a database for rapid on-site inspection. Thus, the fast, accurate, stable POCT approach can be widely applied to monitor drugs and toxins due to its sensitivity, specificity, and abundance database.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135086638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study explores an ultrarapid electrochemical self-doping procedure applied to anodic titanium dioxide (TiO2) nanotube arrays in an alkaline solution to boost their performance for electroanalytical and photocatalytic applications. The electrochemical self-doping process (i.e., the creation of surface Ti3+ states by applying a negative potential) is recently emerging as a simpler and cleaner way to improve the electronic properties of TiO2 compared to traditional chemical and high-temperature doping strategies. Here, self-doping was carried out through varying voltages and treatment times to identify the most performing materials without compromising their structural stability. Interestingly, cyclic voltammetry characterization revealed that undoped TiO2 shows negligible activity, whereas all self-doped materials demonstrate their suitability as electrode materials: an outstandingly short 10 s self-doping treatment leads to the highest electrochemical activity. The electrochemical detection of hydrogen peroxide was assessed as well, demonstrating a good sensitivity and a linear detection range of 3–200 µM. Additionally, the self-doped TiO2 nanotubes exhibited an enhanced photocatalytic activity compared to the untreated substrate: the degradation potential of methylene blue under UV light exposure increased by 25% in comparison to undoped materials. Overall, this study highlights the potential of ultrafast electrochemical self-doping to unleash and improve TiO2 nanotubes performances for electroanalytical and photocatalytic applications.
{"title":"Ultrafast Electrochemical Self-Doping of Anodic Titanium Dioxide Nanotubes for Enhanced Electroanalytical and Photocatalytic Performance","authors":"Davide Spanu, Aicha Dhahri, Gilberto Binda, Damiano Monticelli, Marco Pinna, Sandro Recchia","doi":"10.3390/chemosensors11110560","DOIUrl":"https://doi.org/10.3390/chemosensors11110560","url":null,"abstract":"This study explores an ultrarapid electrochemical self-doping procedure applied to anodic titanium dioxide (TiO2) nanotube arrays in an alkaline solution to boost their performance for electroanalytical and photocatalytic applications. The electrochemical self-doping process (i.e., the creation of surface Ti3+ states by applying a negative potential) is recently emerging as a simpler and cleaner way to improve the electronic properties of TiO2 compared to traditional chemical and high-temperature doping strategies. Here, self-doping was carried out through varying voltages and treatment times to identify the most performing materials without compromising their structural stability. Interestingly, cyclic voltammetry characterization revealed that undoped TiO2 shows negligible activity, whereas all self-doped materials demonstrate their suitability as electrode materials: an outstandingly short 10 s self-doping treatment leads to the highest electrochemical activity. The electrochemical detection of hydrogen peroxide was assessed as well, demonstrating a good sensitivity and a linear detection range of 3–200 µM. Additionally, the self-doped TiO2 nanotubes exhibited an enhanced photocatalytic activity compared to the untreated substrate: the degradation potential of methylene blue under UV light exposure increased by 25% in comparison to undoped materials. Overall, this study highlights the potential of ultrafast electrochemical self-doping to unleash and improve TiO2 nanotubes performances for electroanalytical and photocatalytic applications.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135136261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Geisha arabica coffee from Panama is featured in the world specialty coffee market. Its quality is assessed by sensory analysis with a panel of experts over several days. However, there is a risk of commercial fraud where cheaper coffees are mixed with pure specialty coffees. For these reasons, having an electronic nose (EN) device proves advantageous in supporting the cupping panel. It allows a greater number of fragrance and aroma analyses to be conducted per day, providing more objective results. In this study, an experimental EN equipped with a metal oxide semiconductor (MOS) gas sensor array was used. Olfactory evaluation of ground and infused Geisha coffee of different roast levels, brewing process, and purity was studied by EN, sensory analysis and chromatography. The sensory analysis perceived significant differences in fragrance and aromas in the light and dark roast levels of the samples. A total of 57 volatile organic compounds (VOC) were studied by gas chromatography. The EN data were analyzed chemometrically with principal component analysis (PCA) and predictive partial least squares (PLS). The data variances for two components were explained with values greater than 82%. The EN demonstrated its ability to differentiate the three levels of roasting, two production processes, and adulteration in the analyzed samples.
{"title":"Olfactory Evaluation of Geisha Coffee from Panama Using Electronic Nose","authors":"Nohely Santamaría, Félix Meléndez, Patricia Arroyo, Patricia Calvo, Francisco Sánchez, Jesús Lozano, Ramiro Sánchez","doi":"10.3390/chemosensors11110559","DOIUrl":"https://doi.org/10.3390/chemosensors11110559","url":null,"abstract":"Geisha arabica coffee from Panama is featured in the world specialty coffee market. Its quality is assessed by sensory analysis with a panel of experts over several days. However, there is a risk of commercial fraud where cheaper coffees are mixed with pure specialty coffees. For these reasons, having an electronic nose (EN) device proves advantageous in supporting the cupping panel. It allows a greater number of fragrance and aroma analyses to be conducted per day, providing more objective results. In this study, an experimental EN equipped with a metal oxide semiconductor (MOS) gas sensor array was used. Olfactory evaluation of ground and infused Geisha coffee of different roast levels, brewing process, and purity was studied by EN, sensory analysis and chromatography. The sensory analysis perceived significant differences in fragrance and aromas in the light and dark roast levels of the samples. A total of 57 volatile organic compounds (VOC) were studied by gas chromatography. The EN data were analyzed chemometrically with principal component analysis (PCA) and predictive partial least squares (PLS). The data variances for two components were explained with values greater than 82%. The EN demonstrated its ability to differentiate the three levels of roasting, two production processes, and adulteration in the analyzed samples.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135474866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cadmium (Cd) pollution is an important environmental problem, as it is easily absorbed by plants and gradually accumulates in the human body through the food chain. This study aimed to elucidate the changes in the metabolic response of the rice cultivar “TanLiangYou215” under Cd stress. Rice was grown in soil culture at 0 (Control), 2 (Low group), and 10 (High group) mg/kg CdCl2 for 90 days. The ultrastructural, Cd content, antioxidant activity, and metabolic changes to the rice in different tissues were analyzed. Phenotypic characterization and ultrastructure showed that the rice roots and leaves were significantly damaged and plant growth was inhibited in the High group, while plant growth was promoted in the Low group. Overall, Cd showed a regularity of “low promotion and high inhibition”. Physiological indices revealed that rice was significantly affected by Cd stress. Compared to the Control, Cd stress resulted in higher antioxidant enzyme activities, and the Low group suffered less oxidative damage than the High group. Metabolomic studies revealed that Cd stress significantly altered the metabolic profiles of rice plants. Rice responded to Cd stress by upregulating amino acids and regulating related pathways, including alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. The significant expression of flavonoids with antioxidant properties helped rice resist the oxidative damage caused by Cd accumulation in the root tissue; Cd stress significantly downregulated glycerophospholipid metabolism in the stem and leaf tissues, which affected the cellular activities in rice stem and leaf tissues. We investigated the effects of Cd stress on ultrastructure, antioxidant activity, and metabolic changes in different tissues of the rice variety TLY215. Moreover, the different tissues of TLY215 can regulate these metabolic pathways to resist Cd stress, which provides valuable insights into the response of TLY215 to different concentrations of Cd.
{"title":"Assessing the Effects of Cadmium Stress on the Growth, Physiological Characteristics, and Metabolic Profiling of Rice (Oryza sativa L.) Using HPLC-QTOF/MS","authors":"Zhenni Lan, Qing He, Mingxia Zhang, Huahong Liu, Liusen Fang, Jinfang Nie","doi":"10.3390/chemosensors11110558","DOIUrl":"https://doi.org/10.3390/chemosensors11110558","url":null,"abstract":"Cadmium (Cd) pollution is an important environmental problem, as it is easily absorbed by plants and gradually accumulates in the human body through the food chain. This study aimed to elucidate the changes in the metabolic response of the rice cultivar “TanLiangYou215” under Cd stress. Rice was grown in soil culture at 0 (Control), 2 (Low group), and 10 (High group) mg/kg CdCl2 for 90 days. The ultrastructural, Cd content, antioxidant activity, and metabolic changes to the rice in different tissues were analyzed. Phenotypic characterization and ultrastructure showed that the rice roots and leaves were significantly damaged and plant growth was inhibited in the High group, while plant growth was promoted in the Low group. Overall, Cd showed a regularity of “low promotion and high inhibition”. Physiological indices revealed that rice was significantly affected by Cd stress. Compared to the Control, Cd stress resulted in higher antioxidant enzyme activities, and the Low group suffered less oxidative damage than the High group. Metabolomic studies revealed that Cd stress significantly altered the metabolic profiles of rice plants. Rice responded to Cd stress by upregulating amino acids and regulating related pathways, including alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. The significant expression of flavonoids with antioxidant properties helped rice resist the oxidative damage caused by Cd accumulation in the root tissue; Cd stress significantly downregulated glycerophospholipid metabolism in the stem and leaf tissues, which affected the cellular activities in rice stem and leaf tissues. We investigated the effects of Cd stress on ultrastructure, antioxidant activity, and metabolic changes in different tissues of the rice variety TLY215. Moreover, the different tissues of TLY215 can regulate these metabolic pathways to resist Cd stress, which provides valuable insights into the response of TLY215 to different concentrations of Cd.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-06DOI: 10.3390/chemosensors11110556
Juan Liu, Peng-Yu Cheng, Sai Chen, Meng Wang, Kai Wei, Yuan Li, Yao-Yao Cao, Xing Wang, Hong-Lei Li
A fluorescent probe, N′-((3-methyl-5-oxo-1-phenyl-4, 5-dihydro-1H-pyrazol-4-yl) methylene)-2-oxo-2H-chromene-3-carbohydrazide (MPMC), was synthesized and characterized. Characterizations of the synthetic MPMC were conducted via proton nuclear magnetic resonance (1HNMR) spectroscopy and carbon-13 nuclear magnetic resonance spectroscopy (13C NMR). The fluorescence emission behaviors of the MPMC probe towards diverse metal ions were detected, and the probe exhibited high sensitivity and selectivity towards Cu2+ over other metal ions via the quenching of its fluorescence. Furthermore, the existence of other metal actions made no apparent difference to the fluorescence intensity of the MPMC-Cu2+ system; that is, MPMC displayed a good anti-interference ability. Job’s plot of the MPMC and copper ions indicated that the detection limit was 10.23 nM (R2 = 0.9612) for the assayed actions, with a stoichiometric ratio of 1:1 for MPMC and Cu2+. Additionally, the color of the MPMC probe solution changed from nearly colorless to yellow in the presence of Cu2+ in visible light, and the color change could be observed by the naked eye. Similarly, the color resolved from bright yellow into blue in ultraviolet light. Moreover, reusability studies indicated that the MPMC probe was reusable. The pH effect of the MPMC probe on Cu2+ had a broad range of pH detection, i.e., from 4.0 to 11.0. The response time of the MPMC probe for determining Cu2+ was within 1 min. The recognition of Cu2+ via MPMC performed on pre-treated paper under sunlight and UV light both had a distinct colour change. Thus, the solid-state method for detecting Cu2+ with the naked eye was both economical and convenient.
{"title":"Preparation and Application of a Fast, Naked-Eye, Highly Selective, and Highly Sensitive Fluorescent Probe of Schiff Base for Detection of Cu2+","authors":"Juan Liu, Peng-Yu Cheng, Sai Chen, Meng Wang, Kai Wei, Yuan Li, Yao-Yao Cao, Xing Wang, Hong-Lei Li","doi":"10.3390/chemosensors11110556","DOIUrl":"https://doi.org/10.3390/chemosensors11110556","url":null,"abstract":"A fluorescent probe, N′-((3-methyl-5-oxo-1-phenyl-4, 5-dihydro-1H-pyrazol-4-yl) methylene)-2-oxo-2H-chromene-3-carbohydrazide (MPMC), was synthesized and characterized. Characterizations of the synthetic MPMC were conducted via proton nuclear magnetic resonance (1HNMR) spectroscopy and carbon-13 nuclear magnetic resonance spectroscopy (13C NMR). The fluorescence emission behaviors of the MPMC probe towards diverse metal ions were detected, and the probe exhibited high sensitivity and selectivity towards Cu2+ over other metal ions via the quenching of its fluorescence. Furthermore, the existence of other metal actions made no apparent difference to the fluorescence intensity of the MPMC-Cu2+ system; that is, MPMC displayed a good anti-interference ability. Job’s plot of the MPMC and copper ions indicated that the detection limit was 10.23 nM (R2 = 0.9612) for the assayed actions, with a stoichiometric ratio of 1:1 for MPMC and Cu2+. Additionally, the color of the MPMC probe solution changed from nearly colorless to yellow in the presence of Cu2+ in visible light, and the color change could be observed by the naked eye. Similarly, the color resolved from bright yellow into blue in ultraviolet light. Moreover, reusability studies indicated that the MPMC probe was reusable. The pH effect of the MPMC probe on Cu2+ had a broad range of pH detection, i.e., from 4.0 to 11.0. The response time of the MPMC probe for determining Cu2+ was within 1 min. The recognition of Cu2+ via MPMC performed on pre-treated paper under sunlight and UV light both had a distinct colour change. Thus, the solid-state method for detecting Cu2+ with the naked eye was both economical and convenient.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135589587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-06DOI: 10.3390/chemosensors11110557
Antra Ganguly, Anirban Paul, Shalini Prasad
Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNPs together in a ZIF-8 matrix, and explored the characteristic properties of this novel material composite using standard analytical techniques and its application for biosensor application. The as-synthesized material, duly characterized using various physicochemical analytical methods, portrays the characteristics of the unique encapsulation strategy to develop the first-of-a-kind aptamer and AuNP encapsulation. Non-faradaic Electrochemical Impedance Spectroscopy (EIS) and Chronoamperometry were used to characterize the interfacial electrochemical properties. The biosensor performance was first validated using artificial urine in a controlled buffer medium. The stability and robustness were tested using a real human urine medium without filtration or sample treatment. Being versatile, this Ukrainian-art-inspired biosensor can potentially move the needle towards developing the next generation of sample-in-result-out robust POC diagnostics.
{"title":"Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing","authors":"Antra Ganguly, Anirban Paul, Shalini Prasad","doi":"10.3390/chemosensors11110557","DOIUrl":"https://doi.org/10.3390/chemosensors11110557","url":null,"abstract":"Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNPs together in a ZIF-8 matrix, and explored the characteristic properties of this novel material composite using standard analytical techniques and its application for biosensor application. The as-synthesized material, duly characterized using various physicochemical analytical methods, portrays the characteristics of the unique encapsulation strategy to develop the first-of-a-kind aptamer and AuNP encapsulation. Non-faradaic Electrochemical Impedance Spectroscopy (EIS) and Chronoamperometry were used to characterize the interfacial electrochemical properties. The biosensor performance was first validated using artificial urine in a controlled buffer medium. The stability and robustness were tested using a real human urine medium without filtration or sample treatment. Being versatile, this Ukrainian-art-inspired biosensor can potentially move the needle towards developing the next generation of sample-in-result-out robust POC diagnostics.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135589435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-06DOI: 10.3390/chemosensors11110555
Bing Jin, Jing Liu, Longsheng Jin, Weishuai Liu, Xiangjun Liu
Peroxynitrite (ONOO−) has been revealed to play crucial roles in many physiological and pathological processes, and many diseases were proven to be associated with its misregulated production. The development of fluorescent probes meets the need for tracking ONOO− and gives a better understanding of its diverse mechanisms. In this work, a red-emitting fluorescent probe BP-ONOO was synthesized via functionalization of the rhodol-like fluorophore with a reactive site of hydrazide. The probe BP-ONOO exhibited high sensitivity, excellent selectivity, and short response time (less than 4 s) towards ONOO− under neutral or weak alkaline conditions. These attractive properties favor its application in real-time imaging of ONOO− in living cells, and the probe has been successfully applied for imaging the concentration levels of ONOO− in RAW 264.7 macrophage cells under drug stimulation.
{"title":"A Red-Emitting Fluorescence Probe for Rapid Detecting Exogenous and Endogenous Peroxynitrite in Living Cells with High Sensitivity and Selectivity","authors":"Bing Jin, Jing Liu, Longsheng Jin, Weishuai Liu, Xiangjun Liu","doi":"10.3390/chemosensors11110555","DOIUrl":"https://doi.org/10.3390/chemosensors11110555","url":null,"abstract":"Peroxynitrite (ONOO−) has been revealed to play crucial roles in many physiological and pathological processes, and many diseases were proven to be associated with its misregulated production. The development of fluorescent probes meets the need for tracking ONOO− and gives a better understanding of its diverse mechanisms. In this work, a red-emitting fluorescent probe BP-ONOO was synthesized via functionalization of the rhodol-like fluorophore with a reactive site of hydrazide. The probe BP-ONOO exhibited high sensitivity, excellent selectivity, and short response time (less than 4 s) towards ONOO− under neutral or weak alkaline conditions. These attractive properties favor its application in real-time imaging of ONOO− in living cells, and the probe has been successfully applied for imaging the concentration levels of ONOO− in RAW 264.7 macrophage cells under drug stimulation.","PeriodicalId":10057,"journal":{"name":"Chemosensors","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135635442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-05DOI: 10.3390/chemosensors11110554
Samuel Adesoye, Saqer Al Abdullah, Anjali Kumari, Gayani Pathiraja, Kyle Nowlin, Kristen Dellinger
Developing a biomolecular detection method that minimizes photodamage while preserving an environment suitable for biological constituents to maintain their physiological state is expected to drive new diagnostic and mechanistic breakthroughs. In addition, ultra-sensitive diagnostic platforms are needed for rapid and point-of-care technologies for various diseases. Considering this, surface-enhanced Raman scattering (SERS) is proposed as a non-destructive and sensitive approach to address the limitations of fluorescence, electrochemical, and other optical detection techniques. However, to advance the applications of SERS, novel approaches that can enhance the signal of substrate materials are needed to improve reproducibility and costs associated with manufacture and scale-up. Due to their physical properties and synthesis, semiconductor-based nanostructures have gained increasing recognition as SERS substrates; however, low signal enhancements have offset their widespread adoption. To address this limitation and assess the potential for use in biological applications, zinc oxide (ZnO) was coated with different concentrations (0.01–0.1 M) of gold (Au) precursor. When crystal violet (CV) was used as a model target with the synthesized substrates, the highest enhancement was obtained with ZnO coated with 0.05 M Au precursor. This substrate was subsequently applied to differentiate exosomes derived from three cell types to provide insight into their molecular diversity. We anticipate this work will serve as a platform for colloidal hybrid SERS substrates in future bio-sensing applications.
开发一种生物分子检测方法，将光损伤降到最低，同时保留适合生物成分维持其生理状态的环境，有望推动新的诊断和机制突破。此外，各种疾病的快速和即时护理技术需要超灵敏的诊断平台。考虑到这一点，表面增强拉曼散射(SERS)被提出作为一种非破坏性和敏感的方法来解决荧光，电化学和其他光学检测技术的局限性。然而，为了推进SERS的应用，需要新的方法来增强衬底材料的信号，以提高与制造和放大相关的可重复性和成本。由于其物理性质和合成方法，半导体纳米结构作为SERS衬底得到了越来越多的认可;然而，低信号增强抵消了它们的广泛采用。为了解决这一限制并评估其在生物应用中的潜力，我们在氧化锌(ZnO)表面涂上不同浓度(0.01-0.1 M)的金(Au)前驱体。以结晶紫(CV)为模型靶，在ZnO表面包覆0.05 M Au前驱体时，增强效果最好。该底物随后被用于区分来自三种细胞类型的外泌体，以深入了解其分子多样性。我们预计这项工作将作为胶体混合SERS基板在未来生物传感应用中的平台。
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Pub Date : 2023-11-03DOI: 10.3390/chemosensors11110553
Fanli Meng, Zhenyu Yuan, Dan Meng
The detection of volatile organic compounds (VOCs) is in high demand in various fields, such as environmental pollution monitoring, early disease screening, and food freshness assessment [...]
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