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Determining the earliest growth stage to detect the presence of endophytes in tall fescue and perennial ryegrass seedlings using molecular markers 利用分子标记确定高羊茅和多年生黑麦草幼苗最早生长阶段以检测内生菌的存在
Pub Date : 2023-06-24 DOI: 10.1002/glr2.12053
Kendall Lee, Nicholas Hill, Chloe Dela Cerna, Ali Missaoui

Background

Tall fescue (Festuca arundinacea [Schreb.], Lolium arundinaceum [Schreb.] Darbysh) and perennial ryegrass (Lolium perenne) are important cool-season forage and amenity grasses that have a mutualistic association with an endophytic fungus. Endophytes confer insect and drought resistance to plants but can produce mammalian toxins. Novel endophytes that do not produce mammalian toxins have been introduced to elite cultivars for commercial production. Seed companies need to maintain adequate levels of novel endophytes within the elite forage cultivars. Endophyte detection is performed using immunochemical and molecular techniques because of their speed and reliability. Early detection in seedlings is essential to evaluate the viability of the endophyte within seed lots.

Methods

This research aimed to identify the earliest growth stage in which immunochemical and molecular methods can detect viable endophyte in seedlings of tall fescue cultivars BarOptima (e34), Texoma MaxQII (584), and Jesup MaxQ (542), as well as the perennial ryegrass cultivar Remington (NEA2).

Results

Immunochemical testing detected endophytes in seedlings 14 days after germination (DAG), but the detection rate increased until 42 DAG in some cultivars tested. The molecular marker Tef1exon detected endophytes at a lower rate than the immunochemical method at 28–42 DAG. However, there was insufficient DNA to detect endophytes in 14 DAG seedlings using markers.

Conclusions

We conclude that the most accurate detection of viable endophytes in seedlings was 42 DAG, at which sufficient and consistent endophyte colonization occurred.

背景高羊茅(Festuca arundinacea[Shreb.],Lolium arundinaceam[Shreb.]Darbysh)和多年生黑麦草(Lolium perenne)是重要的冷季牧草和舒适草,与内生真菌有互惠关系。内生植物赋予植物昆虫和抗旱性,但可以产生哺乳动物毒素。不产生哺乳动物毒素的新型内生菌已被引入优良品种中进行商业生产。种子公司需要在优质饲料品种中保持足够水平的新型内生菌。内生菌检测是使用免疫化学和分子技术进行的,因为它们的速度和可靠性。幼苗中的早期检测对于评估种子批内生菌的生存能力至关重要。方法本研究旨在确定免疫化学和分子方法可以检测高羊茅品种BarOptima(e34)、Texoma MaxQII(584)和Jesup MaxQ(542)以及多年生黑麦草品种Remington(NEA2)幼苗中活内生菌的最早生长阶段。结果免疫化学检测在发芽后14天(DAG)幼苗中检测到内生菌,但部分品种的检测率一直上升到42天。分子标记Tef1外显子在28–42 DAG时检测内生菌的比率低于免疫化学方法。然而,使用标记物检测14株DAG幼苗内生菌的DNA不足。结论我们得出的结论是,对幼苗中有活力的内生菌的最准确检测是42DAG,在该条件下,内生菌发生了充分和一致的定殖。
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引用次数: 0
A comparison of the nutritive value of perennial ryegrass, perennial ryegrass and white clover and multispecies-based farmlet systems 多年生黑麦草、多年生黑麦草和白三叶草与多品种农田系统营养价值的比较
Pub Date : 2023-06-24 DOI: 10.1002/glr2.12052
Shona Baker, Mary B. Lynch, Fionnuala Godwin, Tommy M. Boland, Alexander C. O. Evans, Paul N. C. Murphy, Alan K. Kelly, Helen Sheridan

Background

Limited information is available regarding the nutritive value and structural characteristics of multispecies swards when rotationally grazed.

Methods

Three farmlet types were investigated in terms of their nutritive value: Lolium perenne (LP); L. perenne+Trifolium repens (LP+TR); and a multispecies sward containing grasses, legumes and herbs (MSS). Farmlets were stocked with beef steers (2.5 livestock units ha−1), grazed on a 1 ha scale to 6 cm (MSS) and 4 cm (LP and LP+TR) residuals.

Results

A greater ash concentration was found in MSS than LP (84 vs. 75 g kg−1 DM). Both LP+TR and MSS had higher crude protein and lower neutral detergent fibre concentrations than LP. The relative ranking of water soluble carbohydrate and dry matter (DM) concentration was LP > LP+TR > MSS. Despite the leaf component of the LP farmlet contributing more to herbage DM, the organic matter digestibilities of the swards were similar.

Conclusions

The MSS and LP+TR farmlets achieved similar forage quality under intensive grazing to LP. However, it is worth noting that MSS had a higher concentration of acid detergent lignin, which can negatively impact digestibility. Further investigation is needed to determine optimal grazing management practices that can minimise the effects of higher lignin concentration on digestibility in multispecies swards.

背景可获得的关于多物种草地在轮作放牧时的营养价值和结构特征的信息有限。方法对三种农家乐的营养价值进行调查:多年生黑麦草(LP);L.perenne+Trifolium repens(LP+TR);以及含有草、豆类和草本植物的多品种草地(MSS)。农场饲养了肉牛(2.5头 单位ha−1),在1 公顷至6 cm(MSS)和4 cm(LP和LP+TR)残差。结果MSS中的灰分浓度高于LP(84 vs.75 g kg−1 DM)。LP+TR和MSS均具有比LP更高的粗蛋白和更低的中性洗涤纤维浓度。水溶性碳水化合物和干物质(DM)浓度的相对排名为LP >; LP+TR >; MSS。尽管LP农场的叶片成分对牧草DM的贡献更大,但草地的有机物消化能力相似。结论MSS和LP+TR小农场在集约放牧条件下获得了与LP相似的饲料质量。然而,值得注意的是,MSS具有较高浓度的酸性洗涤剂木质素,这会对消化率产生负面影响。需要进一步的研究来确定最佳的放牧管理实践,以最大限度地减少木质素浓度对多品种草地消化率的影响。
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引用次数: 0
Forage breeding and cultivar development: A 50-year perspective 牧草育种与品种发展:50年展望
Pub Date : 2023-06-13 DOI: 10.1002/glr2.12050
Joseph Henry Bouton

This article represents the perspective of one retired, academic forage breeder and cultivar developer after 50 years of working experience. Developing cultivars that help farmers within pastoral agriculture is the main goal, a system where livestock is the consumer. International Grassland Congress proceedings, as well as the general literature, are historical guides for the state of forage breeding. Efficiency was achieved through technologies; better equipment for planting and harvesting; and advances in computer computation and communication. Biotechnology achievements were fitful and continue to evolve. Cultivar performance mean-reversion, cost to the program, and what the farmer seed buyer was willing to pay are important considerations for applying any technology, especially biotechnologies. Biotech promises were too optimistic. This was due, in part, to a lack of understanding that traditional phenotypic/genotypic field selection programs operate in a complex way with multiple species and several traits screened simultaneously at a modest cost. The majority of current forage cultivars are from field-based selection. Industry participation at scientific conferences declined over time, with less sharing of information the result. Cultivar developers will continue using basic field selection methods but should explore applying any technology; just be clever on when, how, and with whom to use them. Practical advice and experiences are also presented and discussed.

这篇文章代表了一位退休的学术饲养员和品种开发人员在50年的工作经验后的观点。开发有助于畜牧农业农民的品种是主要目标,畜牧业是畜牧业的消费者。国际草原大会会议记录以及一般文献都是牧草育种状况的历史指南。效率是通过技术实现的;更好的种植和收割设备;以及计算机计算和通信的进步。生物技术的成就时断时续,并在不断发展。品种性能均值回归、项目成本以及农民种子购买者愿意支付的费用是应用任何技术,特别是生物技术的重要考虑因素。生物技术的承诺过于乐观。这在一定程度上是由于缺乏对传统表型/基因型田间选择程序以复杂的方式运作的理解,即以适度的成本同时筛选多个物种和几个性状。目前的大多数饲草品种都来自于田间选择。行业对科学会议的参与度随着时间的推移而下降,结果是信息共享减少。Cultivar开发人员将继续使用基本的田地选择方法,但应探索应用任何技术;只要在何时使用、如何使用以及与谁一起使用它们方面要聪明。还介绍和讨论了实际的建议和经验。
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引用次数: 0
Nutritive value of forages consumed by ruminants during the dry season in the Western Highlands of Cameroon 喀麦隆西部高地旱季反刍动物消耗的牧草的营养价值
Pub Date : 2023-06-09 DOI: 10.1002/glr2.12051
Leslie Tieubou Tsopgni, Jules Lemoufouet, Felix Meutchieye, Langston Wilfried Edie Nounamo, Camile Nyembo Kondo, Jean Raphaël Kana, Mama Mouchili, Back Armel Feudjio

Background

In the Western Highlands of Cameroon (WHC), information on the nutritional value of fodder species consumed by ruminants is very limited.

Methods

Through interviews with farmers and monitoring of animals on the range, information was obtained on the types of fodder resources consumed by the ruminants. Samples of each forage species were collected in 15 districts, mixed, chopped, and dried in a ventilated oven at 60°C, and then ground for chemical composition analysis.

Results

Twenty-two forage species were identified. Among these species, Vernonia amygdalina (29.43% ± 0.45% dry matter [DM]) and Pennisetum clandestinum (87.21% ± 1.33% DM) were, respectively, the highest in protein and neutral detergent fiber contents. Manihot esculenta was one of the most energy-rich forages in terms of forage unit for lactation and forage unit for meat production. Hierarchical ascending classification revealed three main groups of forages, respectively, rich in crude fiber (Group 1), protein (Group 2), and energy (Group 3).

Conclusions

The present study identified 22 forage species browsed by ruminants in WHC. Further studies should be carried out to determine the antinutritional factors and to evaluate their nutrient value using in vitro or in situ digestibility techniques.

背景在喀麦隆西部高地(WHC),关于反刍动物食用的饲料物种的营养价值的信息非常有限。方法通过对农户的访谈和对牧场动物的监测,了解反刍动物消耗的饲料资源类型。在15个地区采集每种牧草的样本,在60°C的通风烘箱中混合、切碎和干燥,然后研磨进行化学成分分析。结果共鉴定出牧草22种。在这些物种中,杏仁核Veronia(29.43%) ± 0.45%干物质[DM])和狼尾草(87.21% ± 1.33%DM)的蛋白质和中性洗涤剂纤维含量最高。就哺乳用饲料单位和肉类生产用饲料单位而言,木薯是能量最丰富的饲料之一。分级升序分类揭示了三类主要的牧草,分别富含粗纤维(第1组)、蛋白质(第2组)和能量(第3组)。结论本研究确定了反刍动物在WHC中觅食的22种牧草。应进行进一步的研究,以确定抗营养因子,并使用体外或原位消化技术评估其营养价值。
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引用次数: 0
Self-stabilizing maintenance process in plant communities of alpine meadows under different grazing intensities 不同放牧强度下高寒草甸植物群落的自稳定维持过程
Pub Date : 2023-06-05 DOI: 10.1002/glr2.12046
Li Lin, Xingliang Xu, Guangmin Cao, Fawei Zhang, Yikang Li, Junjie Huang

Backgrounds

Grazing prohibition and reduced grazing intensity, as two important “vegetation close-to-nature recovery” methods, have been suggested as economical and effective technologies for enhancing forage production. However, numerous studies have found that the yield of forage could be increased by removing or reducing grazing in a short time in some steady stage of alpine Kobresia meadows, but not in others. To reveal the mechanism behind this phenomenon, we proposed a series of experiments.

Methods

We monitored the plant and soil characteristics in the key steady stages of Kobresia meadows under reduced and prohibited grazing conditions in the same geographic and climatic environments in the northeastern Qinghai-Tibet Plateau for 6 years. We estimated the relationships between the plant community and soil nutrients and obtained the following results.

Results

All measured variables were positively correlated with each other. The plant community structure had higher path coefficients to aboveground biomass, soil organic matter, total nitrogen, and nitrate nitrogen than to other factors. The plant community structure played an important role in response to grazing intensity. Different plant functional groups (PFGs) had different responses to grazing intensity, which led to plant community re-establishment or re-organization under different grazing intensities. Poaceae and Kobresia were more sensitive to grazing intensity than other PFGs, and the ratio of Kobresia biomass (including Kobresia humilis and Kobresia pygmaea) to the total biomass of Poaceae and Kobresia could be used as an indicator of regime shifts within plant communities. With Kobresia pygmaea as the dominant species, the prohibition of grazing was not an efficient approach to increase the yield in the steady stages because this treatment needed more time to recover aboveground biomass. If Poaceae is the dominant PFG, grazing should only be prohibited for 3 years in the steady stages because the aboveground biomass will decrease if grazing is prohibited for more than 3 years.

Conclusions

Therefore, the different steady stages of alpine meadows require different recovery methods to increase recovery efficiency and speed.

背景禁牧和降低放牧强度作为两种重要的“植被接近自然恢复”方法,被认为是提高牧草产量的经济有效的技术。然而,许多研究发现,在高山嵩草草甸的某些稳定阶段,通过在短时间内取消或减少放牧可以提高牧草产量,但在其他阶段则不然。为了揭示这种现象背后的机制,我们提出了一系列实验。方法对青藏高原东北部相同地理气候环境下,禁牧和减牧条件下嵩草草甸关键稳定期的植物和土壤特征进行了6年的监测。我们估计了植物群落与土壤养分之间的关系,并获得了以下结果。结果所有测量变量均呈正相关。植物群落结构对地上生物量、土壤有机质、总氮和硝态氮的通径系数高于其他因子。植物群落结构对放牧强度的响应起着重要作用。不同的植物功能群对放牧强度有不同的反应,导致不同放牧强度下植物群落的重建或重新组织。与其他PFG相比,Poaceae和Kobresia对放牧强度更敏感,并且Kobresia生物量(包括矮嵩草和矮嵩草)与Poaceae and Kobresia总生物量的比率可以作为植物群落内制度变化的指标。由于矮嵩草是优势物种,在稳定阶段禁止放牧并不是提高产量的有效方法,因为这种处理需要更多的时间来恢复地上生物量。如果禾本科植物是主要的PFG,则在稳定阶段只应禁止放牧3年,因为如果禁止放牧超过3年,地上生物量将减少。结论高山草甸不同的稳定阶段需要不同的恢复方法,以提高恢复效率和速度。
{"title":"Self-stabilizing maintenance process in plant communities of alpine meadows under different grazing intensities","authors":"Li Lin,&nbsp;Xingliang Xu,&nbsp;Guangmin Cao,&nbsp;Fawei Zhang,&nbsp;Yikang Li,&nbsp;Junjie Huang","doi":"10.1002/glr2.12046","DOIUrl":"https://doi.org/10.1002/glr2.12046","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Backgrounds</h3>\u0000 \u0000 <p>Grazing prohibition and reduced grazing intensity, as two important “vegetation close-to-nature recovery” methods, have been suggested as economical and effective technologies for enhancing forage production. However, numerous studies have found that the yield of forage could be increased by removing or reducing grazing in a short time in some steady stage of alpine <i>Kobresia</i> meadows, but not in others. To reveal the mechanism behind this phenomenon, we proposed a series of experiments.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We monitored the plant and soil characteristics in the key steady stages of <i>Kobresia</i> meadows under reduced and prohibited grazing conditions in the same geographic and climatic environments in the northeastern Qinghai-Tibet Plateau for 6 years. We estimated the relationships between the plant community and soil nutrients and obtained the following results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All measured variables were positively correlated with each other. The plant community structure had higher path coefficients to aboveground biomass, soil organic matter, total nitrogen, and nitrate nitrogen than to other factors. The plant community structure played an important role in response to grazing intensity. Different plant functional groups (PFGs) had different responses to grazing intensity, which led to plant community re-establishment or re-organization under different grazing intensities. Poaceae and <i>Kobresia</i> were more sensitive to grazing intensity than other PFGs, and the ratio of <i>Kobresia</i> biomass (including <i>Kobresia humilis</i> and <i>Kobresia pygmaea</i>) to the total biomass of Poaceae and <i>Kobresia</i> could be used as an indicator of regime shifts within plant communities. With <i>Kobresia pygmaea</i> as the dominant species, the prohibition of grazing was not an efficient approach to increase the yield in the steady stages because this treatment needed more time to recover aboveground biomass. If Poaceae is the dominant PFG, grazing should only be prohibited for 3 years in the steady stages because the aboveground biomass will decrease if grazing is prohibited for more than 3 years.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Therefore, the different steady stages of alpine meadows require different recovery methods to increase recovery efficiency and speed.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100593,"journal":{"name":"Grassland Research","volume":"2 2","pages":"140-152"},"PeriodicalIF":0.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glr2.12046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50121887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tiller demography of tall fescue and tall wheatgrass cut at different times during the reproductive phase 高羊茅和高麦草生育期不同时期分蘖的人口学特征
Pub Date : 2023-05-24 DOI: 10.1002/glr2.12047
Jorge O. Scheneiter, Matias Sacoccia, María J. Beribe, Pablo Barletta

Background

Tall fescue (TF) and tall wheatgrass (TW) are the main temperate perennial grasses used for livestock grazing in the Pampas region of Argentina. In this region, pastures based on these grasses show low tiller density and the presence of bare soil in winter.

Methods

An experiment was performed in pots kept outdoors. Plants of TF and TW were cut every 550°C day and 700°C day, respectively. In each species, reproductive tillers were removed at different times: (i) at each cut, (ii) when they reached the flag leaf stage, or (iii) when they reached full bloom. Tiller appearance, tiller death, and type of bud (basal or axillary) of the tillers present were determined.

Results

Tiller appearance decreased from July to April. Minimum values were 0.2 and 1.1 tillers 100 tillers−1 day−1 in TF and TW, respectively. In TF, tiller death began in September–October and increased from 0.2 to 0.8 tillers 100 tillers−1 day−1 in March–April. In TW, tiller death began in October–November and increased from 0.6 to 0.8–1.0 tillers 100 tillers−1 day−1 from February to April.

Conclusions

Defoliation of reproductive tillers affected the tiller density of TF plants in spring and in the following fall. In TW, the treatments did not modify the tiller density of the plants.

背景高羊茅(TF)和高麦草(TW)是阿根廷潘帕斯地区主要的温带多年生牧草。在该地区,以这些草为基础的牧场在冬季表现出较低的分蘖密度和裸露的土壤。方法采用户外盆栽实验。TF和TW的植株分别每550°C天和700°C天切割一次。在每个物种中,生殖分蘖在不同的时间被移除:(i)在每次切割时,(ii)当它们到达旗叶期时,或(iii)当它们达到盛开时。测定了分蘖的出现、分蘖死亡和分蘖的芽类型(基部或腋生)。结果7~4月分蘖出现率下降。最小值分别为0.2和1.1 分蘖 100 分蘖−1 TF和TW分别为第−1天。在TF中,分蘖死亡开始于9-10月,从0.2增加到0.8 分蘖 100 分蘖−1 三月至四月的第一天。在TW,分蘖死亡始于10月至11月,从0.6增加到0.8–1.0 分蘖 100 分蘖−1 二月至四月的第−1天。结论繁殖分蘖的落叶影响TF植株春季和秋季的分蘖密度。在TW,处理并没有改变植株的分蘖密度。
{"title":"Tiller demography of tall fescue and tall wheatgrass cut at different times during the reproductive phase","authors":"Jorge O. Scheneiter,&nbsp;Matias Sacoccia,&nbsp;María J. Beribe,&nbsp;Pablo Barletta","doi":"10.1002/glr2.12047","DOIUrl":"https://doi.org/10.1002/glr2.12047","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Tall fescue (TF) and tall wheatgrass (TW) are the main temperate perennial grasses used for livestock grazing in the Pampas region of Argentina. In this region, pastures based on these grasses show low tiller density and the presence of bare soil in winter.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>An experiment was performed in pots kept outdoors. Plants of TF and TW were cut every 550°C day and 700°C day, respectively. In each species, reproductive tillers were removed at different times: (i) at each cut, (ii) when they reached the flag leaf stage, or (iii) when they reached full bloom. Tiller appearance, tiller death, and type of bud (basal or axillary) of the tillers present were determined.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Tiller appearance decreased from July to April. Minimum values were 0.2 and 1.1 tillers 100 tillers<sup>−1</sup> day<sup>−1</sup> in TF and TW, respectively. In TF, tiller death began in September–October and increased from 0.2 to 0.8 tillers 100 tillers<sup>−1</sup> day<sup>−1</sup> in March–April. In TW, tiller death began in October–November and increased from 0.6 to 0.8–1.0 tillers 100 tillers<sup>−1</sup> day<sup>−1</sup> from February to April.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Defoliation of reproductive tillers affected the tiller density of TF plants in spring and in the following fall. In TW, the treatments did not modify the tiller density of the plants.</p>\u0000 </section>\u0000 </div>","PeriodicalId":100593,"journal":{"name":"Grassland Research","volume":"2 2","pages":"120-128"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/glr2.12047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Elevated and atmospheric-level methane consumption by soil methanotrophs of three grasslands in China 中国三个草原土壤甲烷菌甲烷消耗量的上升和大气水平
Pub Date : 2023-05-15 DOI: 10.1002/glr2.12048
Yufang Wang, Yuanfeng Cai, Fujiang Hou, Saman Bowatte, Zhongjun Jia

Background

Methane (CH4) oxidation driven by soil aerobic methanotrophs demonstrates the capacity of grassland as a CH4 sink.

Methods

In this study, we compared the oxidation characteristics of atmospheric-level and elevated concentration (10%) CH4 in a typical grassland (steppe) on the Loess Plateau, an alpine meadow (meadow) on the Qinghai-Tibet Plateau, and an inland arid-area artificial grassland (pasture) in northwest China and investigated the communities of active methanotrophs and their contribution to CH4 oxidation using DNA-based stable-isotope probing and Illumina Miseq sequencing.

Results

The results showed that the oxidation of atmospheric CH4 only occurred in steppe and meadow soils where the USCγ group of methanotrophs was numerically dominant in the methanotroph community. Pasture soils, with their very low relative abundance of USCγ, did not show atmospheric CH4 oxidation. However, a DNA-stable isotope probing experiment with 10% CH4 indicated that conventional CH4 oxidizers (Methylocaldum and Methylocystis) rather than USCγ communities assimilated significant amounts of 13CH4 for growth.

Conclusions

The CH4 oxidation mechanisms in the three experimental grassland soils varied significantly. The USCγ group may be obligate oligotrophic microorganisms or their growth requires specific unknown conditions.

背景甲烷(CH4)氧化由土壤好氧甲烷菌驱动,表明草地具有CH4汇的能力。方法比较了黄土高原典型草原(草原)、青藏高原高寒草甸(草甸)、,和中国西北内陆干旱区的人工草地(牧场),并利用基于DNA的稳定同位素探测和Illumina Miseq测序研究了活性甲烷菌的群落及其对CH4氧化的贡献。结果大气CH4的氧化仅发生在草原和草甸土壤中,其中甲烷营养菌的USCγ组在甲烷营养菌群落中占主导地位。牧场土壤的USCγ相对丰度非常低,没有表现出大气中CH4的氧化。然而,使用10%CH4的DNA稳定同位素探测实验表明,传统的CH4氧化剂(甲藻和甲藻)而不是USCγ群落吸收了大量的13CH4用于生长。结论三种草地土壤CH4氧化机制存在显著差异。USCγ群可能是专性寡营养微生物,或者它们的生长需要特定的未知条件。
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引用次数: 0
Correction to “Quantifying golf course nitrogen use efficiency” 对“量化高尔夫球场氮利用效率”的更正
Pub Date : 2023-05-03 DOI: 10.1002/glr2.12049

Bekken, M. A. H., & Soldat, D. J. (2022). Quantifying golf course nitrogen use efficiency. Grassland Research, 1(3), 174–186. https://doi.org/10.1002/glr2.12024

There was an error in the variance term used in the growth potential (GP) model in this study. Variances of 10 and 12 were used for C3 and C4 turfgrasses, respectively. These variances are for Fahrenheit temperatures; however, this study used Celsius temperatures. When using Celsius in the equation, it is best to adjust these variance terms to 5.5 for C3 grasses and 7 for C4 grasses. With the updated variances, the definition of a turfgrass growth day also needed to be adjusted. Following the recommendation of Gelernter et al. (2016), we adjusted the definition of a turfgrass growth day to a day in which the growth potential was greater than 20%.

The updated parameters caused small changes to the number of turfgrass growth days calculated at each golf course in the study. This, in turn, changed the N rate normalized by turfgrass growth days, the Nitrogen Efficiency Scores (NES), the Nmax normalized NES, and the correlations between the N rate normalized by turfgrass growth days and the economic factors. Figures 3-9 have been updated and Tables 3–9 have been updated. None of the paper's main conclusions have changed, but a description of the updated results from the original manuscript is included below.

With the updated parameters, the ratio of the golf course-wide N application rate to the GP N requirement prediction (termed the NES) was 0.47, indicating that golf courses used 53% less N than predicted by the model.

With the updated parameters, four (US-East Texas, US-Florida, US-Northwest, Norway) of the eight regions had similar N rates on fairways; median fairway N rates normalized by growth days were between 0.61 and 0.65 kg ha−1 day−1 in these regions. The remaining four regions (Denmark, US-Midwest, US-Northeast, UK) were below this range, with the median fairway N rate normalized by turfgrass growth days between 0.34 and 0.19 kg ha−1 day−1.

With the old parameters, tees in Norway were the only golf course components across all regions for which the median NES was greater than 1. With the updated parameters, US-East Texas, US-Florida, US-Northwest, and Norway had median NES values that were greater than 1 on greens. On tees in Norway, the median NES score remained above 1. In the US-Northwest, the median NES score on tees rose above 1. The median NES score in all other regions across all components stayed below 1.

The updated parameters slightly changed the rankings of NES in Table 9. The ranking of the N rate normalized by turfgrass growth days and the NES of the US-Midwest changed from 6th to 5th and the ranking of the US-Northeast changed from 5th to 6th.

贝克肯,M.A.H.,&;Soldat,D.J.(2022)。量化高尔夫球场的氮利用效率。草原研究,1(3),174–186。https://doi.org/10.1002/glr2.12024There是本研究中生长潜力(GP)模型中使用的方差项中的一个错误。C3和C4草坪草分别使用10和12的变体。这些变化是针对华氏温度;然而,这项研究使用了摄氏度的温度。当在方程中使用摄氏度时,C3草最好将这些方差项调整为5.5,C4草最好将其调整为7。随着方差的更新,草坪草生长日的定义也需要调整。根据Gelernter等人(2016)的建议,我们将草坪草生长日的定义调整为生长潜力大于20%的一天。更新后的参数使研究中每个高尔夫球场计算的草坪草生长天数发生了微小变化。这反过来又改变了由草坪草生长天数归一化的N速率、氮效率得分(NES)、Nmax归一化的NES以及由草坪草增长天数归一化的氮速率与经济因素之间的相关性。图3-9已更新,表3-9也已更新。论文的主要结论没有改变,但以下是对原稿更新结果的描述。使用更新的参数,高尔夫球场全N应用率与GP N需求预测(称为NES)的比率为0.47,表明高尔夫球场使用的N比模型预测的少53%。根据更新的参数,八个地区中的四个(美国得克萨斯州东部、美国佛罗里达州、美国西北部、挪威)在球道上的N率相似;按生长天数标准化的球道N率中值在0.61和0.65之间 公斤 ha−1 这些地区的第−1天。其余四个地区(丹麦、美国中西部、美国东北部、英国)低于这一范围,草坪生长天数归一化后的球道N率中值在0.34至0.19之间 公斤 ha−1 day−1。根据旧参数,挪威的发球台是所有地区唯一一个NES中值大于1的高尔夫球场组成部分。根据更新的参数,美国得克萨斯州东部、佛罗里达州、西北部和挪威的果岭NES中值均大于1。在挪威的发球台上,NES得分中值保持在1分以上。在美国西北部,发球台的NES得分中值上升到1分以上。所有组成部分的所有其他地区的NES得分中值保持在1以下。更新的参数略微改变了表9中NES的排名。美国中西部草坪草生长天数和NES归一化的N率排名从第6位变为第5位,美国东北部的排名从第5位变为6位。
{"title":"Correction to “Quantifying golf course nitrogen use efficiency”","authors":"","doi":"10.1002/glr2.12049","DOIUrl":"https://doi.org/10.1002/glr2.12049","url":null,"abstract":"<p>Bekken, M. A. H., &amp; Soldat, D. J. (2022). Quantifying golf course nitrogen use efficiency. <i>Grassland Research</i>, <i>1</i>(3), 174–186. https://doi.org/10.1002/glr2.12024</p><p>There was an error in the variance term used in the growth potential (GP) model in this study. Variances of 10 and 12 were used for C<sub>3</sub> and C<sub>4</sub> turfgrasses, respectively. These variances are for Fahrenheit temperatures; however, this study used Celsius temperatures. When using Celsius in the equation, it is best to adjust these variance terms to 5.5 for C<sub>3</sub> grasses and 7 for C<sub>4</sub> grasses. With the updated variances, the definition of a turfgrass growth day also needed to be adjusted. Following the recommendation of Gelernter et al. (2016), we adjusted the definition of a turfgrass growth day to a day in which the growth potential was greater than 20%.</p><p>The updated parameters caused small changes to the number of turfgrass growth days calculated at each golf course in the study. This, in turn, changed the N rate normalized by turfgrass growth days, the Nitrogen Efficiency Scores (NES), the <i>N</i><sub>max</sub> normalized NES, and the correlations between the N rate normalized by turfgrass growth days and the economic factors. Figures 3-9 have been updated and Tables 3–9 have been updated. None of the paper's main conclusions have changed, but a description of the updated results from the original manuscript is included below.</p><p>With the updated parameters, the ratio of the golf course-wide N application rate to the GP N requirement prediction (termed the NES) was 0.47, indicating that golf courses used 53% less N than predicted by the model.</p><p>With the updated parameters, four (US-East Texas, US-Florida, US-Northwest, Norway) of the eight regions had similar N rates on fairways; median fairway N rates normalized by growth days were between 0.61 and 0.65 kg ha<sup>−1</sup> day<sup>−1</sup> in these regions. The remaining four regions (Denmark, US-Midwest, US-Northeast, UK) were below this range, with the median fairway N rate normalized by turfgrass growth days between 0.34 and 0.19 kg ha<sup>−1</sup> day<sup>−1</sup>.</p><p>With the old parameters, tees in Norway were the only golf course components across all regions for which the median NES was greater than 1. With the updated parameters, US-East Texas, US-Florida, US-Northwest, and Norway had median NES values that were greater than 1 on greens. On tees in Norway, the median NES score remained above 1. In the US-Northwest, the median NES score on tees rose above 1. The median NES score in all other regions across all components stayed below 1.</p><p>The updated parameters slightly changed the rankings of NES in Table 9. The ranking of the N rate normalized by turfgrass growth days and the NES of the US-Midwest changed from 6th to 5th and the ranking of the US-Northeast changed from 5th to 6th.</p>","PeriodicalId":100593,"journal":{"name":"Grassland Research","volume":"2 3","pages":"220-224"},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71929994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Quantifying golf course nitrogen use efficiency” 对“量化高尔夫球场氮利用效率”的修正
Pub Date : 2023-05-03 DOI: 10.1002/glr2.12049
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引用次数: 0
Breeding for drought tolerance in perennial ryegrass (Lolium perenne L.) and tall fescue (Lolium arundinaceum [Schreb.] Darbysh.) by exploring genotype by environment by management interactions 多年生黑麦草(Lolium perenne L.)和高羊茅(Lonium arundinaceum[Schreb.]Darbysh.)的抗旱性育种
Pub Date : 2023-05-01 DOI: 10.1002/glr2.12045
Claudio C. Fernandes Filho, Mario H. Murad Leite Andrade, Jose A. Rodrigues Nunes, Joseph Wipff, Debra L. Hignight, Esteban F. Rios, Kevin Kenworthy, Kenneth Hignight

Background

Drought-tolerant turfgrass cultivars are becoming more important each year as landscape irrigation practices are becoming more restrictive.

Methods

In this study, we quantified the level of drought tolerance (DT) in perennial ryegrass and tall fescue genotypes across years under different mowing (MH) and fertility (FL) rates. A total of 48 ryegrass and 38 tall fescue genotypes were planted in 2011 at NexGen, Albany, OR. The trial was divided into three subtrials: (i) DT; (ii) MH and DT; and (iii) FL and DT.

Results

The results showed that there was significant genetic variance for most of the traits in both species. The genotype × year interaction was predominantly simple, indicating small changes in genotype ranking for both species. Lower MH improved DT in perennial ryegrass, whereas higher MH resulted in greater DT in tall fescue. For both species, higher MH improved the population mean for turf quality. Higher FL inputs led to higher turf quality for both species, but it did not affect the DT response.

Conclusions

Using a selection index, six genotypes for perennial ryegrass and four for tall fescue were selected.

背景随着景观灌溉实践的限制性越来越强,耐旱草坪草品种每年都变得越来越重要。方法在本研究中,我们量化了多年生黑麦草和高羊茅基因型在不同割草率(MH)和生育率(FL)下的耐旱性(DT)水平。2011年,俄勒冈州奥尔巴尼NexGen共种植了48种黑麦草和38种高羊茅基因型。试验分为三个子项:(i)DT;(ii)MH和DT;以及(iii)FL和DT。结果两个品种的大部分性状均存在显著的遗传变异。基因型 × 年份交互作用主要是简单的,表明两个物种的基因型排名变化较小。较低的MH改善了多年生黑麦草的DT,而较高的MH导致高羊茅的DT更大。对于这两个物种,较高的MH改善了草坪质量的种群平均值。较高的FL输入导致两个物种的草皮质量较高,但不会影响DT反应。结论采用选择指数法筛选出6个多年生黑麦草基因型和4个高羊茅基因型。
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