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Improving bermudagrass in the Southeastern United States with alfalfa as an alternative nitrogen source in grazing systems 在放牧系统中以苜蓿作为替代氮源改良美国东南部的百慕大草
Pub Date : 2023-01-18 DOI: 10.1002/glr2.12038
Justin C. Burt, Lisa L. Baxter, Christopher G. Prevatt, Mary Kimberly Mullenix, R. Lawton Stewart Jr., Jennifer J. Tucker

Background

Bermudagrass (Cynodon spp.) is one of the most common perennial warm-season forages grown in the Southeastern United States. The incorporation of alfalfa (Medicago sativa) into bermudagrass pastures could serve as an alternative source of nitrogen (N) while also improving the nutritive value of the forage base.

Methods

A 2-year grazing evaluation arranged in a randomized complete block design was conducted from May to September 2018 (Year 1) and May–August 2019 (Year 2) in Tifton, GA, USA. The objective was to compare the production of alfalfa–bermudagrass mixed pastures (BGA) with bermudagrass monoculture pastures with (BGN) or without (BG) the application of synthetic nitrogen on forage production and stocker cattle performance.

Results

Differences were observed for average daily gain (p = 0.10), gain per hectare (p = 0.02) and stocking rate (p = 0.01), in that BGA was greatest compared to BGN and BG, which did not differ from each other. Furthermore, BGA had increased forage crude protein (p = 0.01) and total digestible nutrients (p = 0.01) compared to BGN and BG. Finally, BGA provided the greatest economic return per hectare compared to BG or BGN.

Conclusions

These data illustrate improved animal performance and forage nutritive value using alfalfa–bermudagrass systems and offer a viable option for producers seeking alternatives to synthetic N sources for bermudagrass pastures in the Southeastern United States.

百慕大草(Cynodon spp.)是生长在美国东南部最常见的多年生暖季牧草之一。在百米草牧场中添加苜蓿可以作为氮素的替代来源,同时也可以提高牧草基质的营养价值。方法于2018年5月至9月(第1年)和2019年5月至8月(第2年)在美国乔治亚州蒂夫顿进行为期2年的随机完全区组设计放牧评估。目的是比较紫花苜蓿-百德草混合牧场(BGA)与百德草单作牧场(BGA)的产量和合成氮的施用对饲料生产和牛生产性能的影响。结果平均日增重(p = 0.10)、每公顷增重(p = 0.02)和载畜率(p = 0.01)存在差异,其中BGA最大,BGN和BG之间无显著差异。此外,与BGN和BG相比,BGA显著提高了饲料粗蛋白质(p = 0.01)和总可消化养分(p = 0.01)。最后,与BG或BGN相比,BGA提供了每公顷最大的经济回报。这些数据表明,紫花苜蓿-百慕大草系统可以提高动物生产性能和饲料营养价值,并为美国东南部百慕大草牧场的生产者寻找替代合成氮源的可行选择。
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引用次数: 1
Switchgrass sward establishment selection is consistent across multiple environments and fertilization levels 柳枝稷在不同环境和施肥水平下的建树选择是一致的
Pub Date : 2023-01-11 DOI: 10.1002/glr2.12035
Neal W. Tilhou, Michael D. Casler

Background

Strong selection can occur during switchgrass sward establishment. Differences in establishment selection due to environment or management could provide information on genotype-by-environment variation and could influence strategies for breeding perennial grasses.

Methods

Leaf samples were collected before sward establishment and from 3-year-old swards for two breeding groups (lowland and hybrid) at three locations. Within two locations, samples were collected from paired fertilized (112 kg N ha−1) and unfertilized plots. Allele frequencies from pooled DNA samples were studied through multivariate analysis of variance, genomewide trait predictions (heading date and winter survivorship), and genomically estimated breeding values (GEBVs) for individual sward survival within an independent data set.

Results

This study found only minor variations in selection due to location or management. Predicted heading dates of the hybrid population had significant changes due to fertilization and location. There were strong correlations among sward establishment survival GEBVs between growing environments (hybrid r = 0.77; gulf r = 0.97). Interestingly, this study found a small number of genotypes that were over-represented in established swards across all growing environments.

Conclusions

This study reinforces a prior report of selection during sward establishment and indicates that only a small degree of establishment selection is location-specific within these diverse growing conditions.

在柳枝稷生长过程中会发生强选择。由于环境或管理导致的生境选择差异可以提供基因型随环境变化的信息,并可能影响多年生草的育种策略。方法在3个地点采集草地建立前和3年生禾草的叶片样本,分为低地和杂交两个育种组。在两个地点,从成对施肥(112 kg N ha - 1)和未施肥地块采集样本。通过多变量方差分析、全基因组性状预测(抽穗日期和冬季存活率)以及独立数据集中个体swward存活率的基因组估计育种值(GEBVs),研究了混合DNA样本的等位基因频率。结果本研究发现,由于地点或管理,在选择上只有很小的差异。杂交群体的抽穗期预测受施肥和位置的影响有显著的变化。不同生长环境间草种成活gebv有很强的相关性(杂交r = 0.77;海湾r = 0.97)。有趣的是,这项研究发现,在所有生长环境中,少数基因型在已建立的植物中都有过度的代表。本研究强化了先前关于草木生长过程中选择的报道,表明在这些不同的生长条件下,只有一小部分草木生长选择是特定于地点的。
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引用次数: 0
Agronomic assessment of two populations of intermediate wheatgrass—Kernza® (Thinopyrum intermedium) in temperate South America 南美温带两个中间麦草- kernza®(Thinopyrum intermedium)群体的农艺评价
Pub Date : 2023-01-08 DOI: 10.1002/glr2.12032
Andres Locatelli, Lucia Gutierrez, Olivier Duchene, Pablo R. Speranza, Valentin D. Picasso Risso

Background

Kernza® intermediate wheatgrass is a perennial grain and forage crop that can provide several ecosystem services. Major research efforts focused on Kernza have taken place in high latitudes. The goal of this study was to evaluate, for the first time, the agronomic performance of Kernza in a low-latitude region with mild winters.

Methods

A KS-cycle 4 Kernza population (A) was planted in spring in Wisconsin, USA, and selected in one cycle for lower vernalization requirements, obtaining a new population (B). These two populations, at three nitrogen (N) fertilization rates, were evaluated in a full factorial, completely randomized field experiment in Uruguay over 2 years.

Results

The populations were similar in grain yields and flowering time in the 1st year, but population B had 63% lower grain yield in the 2nd year and 20% lower forage yield throughout the experiment. Increasing the N rate to 160 kg ha−1 led to a 63% increase in grain yield and 28% increase in forage yield across populations. Forage yields and nutritive values were similar to those reported in the northern hemisphere. However, grain yields for both the 1st (316 kg ha−1) and 2nd year (41 kg ha−1) were lower due to reduced flowering and weed competition.

Conclusions

Expansion of Kernza to lower-latitude regions will require further breeding to improve reproductive performance.

Kernza®中间小麦草是一种多年生粮食和饲料作物,可以提供多种生态系统服务。主要的研究工作集中在高纬度地区。本研究的目的是首次评价克恩扎在暖冬低纬度地区的农艺表现。方法在美国威斯康辛州春季种植一个KS-cycle 4 Kernza群体(A),在一个周期内选择较低的春化需求,获得一个新群体(B)。在乌拉圭进行为期2年的全因子、完全随机田间试验,对这两个群体在3种氮肥施用量下进行评价。结果2个群体在第1年的粮食产量和开花时间上基本一致,但种群B在第2年的粮食产量比第2年低63%,整个试验期间的牧草产量比第2年低20%。将施氮量提高到160 kg ha - 1,籽粒产量提高63%,牧草产量提高28%。草料产量和营养价值与北半球的报道相似。然而,由于开花和杂草竞争减少,第一年(316 kg ha - 1)和第二年(41 kg ha - 1)的粮食产量都有所下降。结论在低纬度地区扩展Kernza需要进一步的育种来提高繁殖性能。
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引用次数: 1
Morphological and productivity responses of Buffel grass (Cenchrus ciliaris) and Guinea grass (Panicum maximum) ecotypes to simulated grazing in a semi-arid environment 半干旱环境下灰羊草和几内亚草生态型对模拟放牧的形态和生产力响应
Pub Date : 2023-01-02 DOI: 10.1002/glr2.12033
Bosco Kidake Kisambo, Oliver V. Wasonga, Oscar K. Koech, George N. Karuku

Background

Livestock production is an economically significant activity in grassland-based production systems with herbivory, a critical process in modifying plant structure and regulating biomass dynamics in these areas.

Methods

A grazing simulation experiment was undertaken in a semi-arid environment in Kenya to determine the effects of clipping frequency and intensity on the morphology and productivity of Cenchrus ciliaris and Panicum maximum grass ecotypes. The ecotypes were clipped at different frequencies (every 2, 4, and 12 weeks) and different intensities (5, 10, and 15 cm clipping heights). The morphological responses of the grass ecotypes and biomass yields were determined.

Results

The grass ecotypes showed significantly (p < 0.05) varied responses to cutting frequency and intensity. Morphological parameters, tillering, and biomass production were all affected by clipping frequency and intensity. The highest dry matter (DM) yields were recorded under low frequency (LF) clipping, followed by moderate frequency (MF) clipping and high frequency (HF) clipping. LF clipping in C. ciliaris ecotype Kilifi was 20% and 31% more compared to MF and HF clipping. No significant difference in yields was found by cutting the C. ciliaris ecotype Magadi at 4- and 12-week intervals, even though clipping at 2-week intervals cumulatively yielded less biomass. There was a biomass yield reduction in P. maximum ecotype Taveta of 23% and 25% for MF and HF clipping, respectively, while for ecotype Isinya, the reduction was 13% and 15% for MF and HF, respectively. Clipping intensity had a significant (p < 0.05) effect on cumulative yields in C. ciliaris ecotypes, but not P. maximum. Ecotypic differences were apparent in both C. ciliaris and P. maximum ecotype DM yields.

Conclusions

The results in this study suggest that clipping range grass ecotypes less frequently (4- and 12-week intervals) and at 10 and 15 cm stubble heights results in higher biomass yields. Generally, LF and MF grazing and cutting strategies are advantageous for semi-arid lands. Combinations of frequent and high-intensity utilization grazing strategies should be discouraged as they are likely to lead to reduced biomass yields and negative effects both on grasses and on the environment.

畜牧生产是草地生产系统中具有重要经济意义的活动,是改变这些地区植物结构和调节生物量动态的关键过程。方法在肯尼亚半干旱环境下进行模拟放牧试验,研究不同放牧频率和强度对毛帚草和大头草生态型形态和生产力的影响。以不同频率(每2周、4周和12周)和不同强度(5、10和15 cm剪枝高度)剪枝生态型。测定了草的生态类型和生物量的形态响应。结果禾草生态型对刈割频率和刈割强度的响应差异显著(p < 0.05)。刈割频率和刈割强度均影响植株的形态参数、分蘖和生物量。干物质产量以低频刈割最高,其次为中频刈割和高频刈割。与中频和高频剪接相比,基利菲生态型纤毛蝗的低频剪接率分别高出20%和31%。每隔4周和12周进行一次采伐对产量没有显著影响,尽管每隔2周采伐的累积生物量较少。在MF和HF剪切下,最大p生态型Taveta的生物量产量分别降低23%和25%,而在MF和HF剪切下,最大p生态型Isinya的生物量产量分别降低13%和15%。剪断强度对毛菖蒲生态型的累积产量有显著影响(p < 0.05),但对毛菖蒲的累积产量影响不显著。生态型差异在毛菖蒲和毛菖蒲生态型DM产量上均有明显差异。结论剪草频率较低(4周和12周间隔),茬高10和15 cm可获得较高的生物量产量。一般来说,半干旱区采用LF和MF放牧和采伐策略较为有利。应不鼓励频繁和高强度利用放牧战略的结合,因为它们可能导致生物量产量下降,并对草和环境产生负面影响。
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引用次数: 1
Application of CRISPR/Cas9 technology in forages CRISPR/Cas9技术在牧草中的应用
Pub Date : 2023-01-02 DOI: 10.1002/glr2.12036
Qinyan Bao, Tezera W. Wolabu, Qian Zhang, Tian Zhang, Zhipeng Liu, Juan Sun, Zeng-Yu Wang

Genome editing is an advanced genetic modification tool that facilitates the removal, insertion, or substitution of nucleotides in the genome of an organism for mutation induction. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) is the most widely used genome editing tool that can precisely modify the specific sequence of a genome. Significant progress has been made in the use of CRISPR/Cas9 for the genetic improvement of food crops. However, in forage crops, the use of CRISPR/Cas9 is still in the infant stage with limited understanding and application. This article reviews current advances in the establishment and application of the CRISPR/Cas9 system in alfalfa, summarizes the application of CRISPR/Cas9 in several other forage legume and grass species, and discusses the prospects of CRISPR/Cas9 in the genetic improvement of forage crops.

基因组编辑是一种先进的基因修饰工具,它促进了生物体基因组中核苷酸的去除、插入或替代,以诱导突变。CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)是目前应用最广泛的基因组编辑工具,可以精确修饰基因组的特定序列。利用CRISPR/Cas9技术对粮食作物进行遗传改良已取得重大进展。然而,在饲料作物中,CRISPR/Cas9的使用仍处于起步阶段,了解和应用有限。本文综述了目前CRISPR/Cas9系统在苜蓿中的建立和应用进展,总结了CRISPR/Cas9在其他几种饲用豆科植物和禾草中的应用,并对CRISPR/Cas9在饲用作物遗传改良中的应用前景进行了展望。
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引用次数: 1
Plant genome editing: CRISPR, base editing, prime editing, and beyond 植物基因组编辑:CRISPR、碱基编辑、引体编辑等
Pub Date : 2022-12-29 DOI: 10.1002/glr2.12034
Yujie Xie, Syed Inzimam Ul Haq, Xingyu Jiang, Dianfeng Zheng, Naijie Feng, Wenying Wang, Jin-Sheng He, Quan-Sheng Qiu

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a fast-growing, genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding. Here, we summarized recent advances in the development and applications of genome editing technologies in plants. We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology. Some new prime editing-derived techniques were assessed.

CRISPR /CRISPR相关蛋白9 (CRISPR -associated protein 9, Cas9)系统是一种快速发展的基因组编辑技术,在鉴定基因功能以及改善农业生产和作物育种方面有着广泛的应用。本文综述了近年来植物基因组编辑技术的发展和应用进展。我们简要介绍了CRISPR/Cas9技术,并研究了从CRISPR技术发展而来的碱基和引物编辑技术。评估了一些新的原始编辑衍生技术。
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引用次数: 2
Correction to Photothermal controls of vegetative dormancy in Poa secunda 对黄豆营养休眠光热控制的修正
Pub Date : 2022-11-15 DOI: 10.1002/glr2.12031

Chen, A. A., Fei, S.-z., Lenssen, A. W., & Moore, K. J. (2022). Photothermal controls of vegetative dormancy in Poa secunda. Grassland Research, 1(1), 43–52. https://doi.org/10.1002/glr2.12008

There is an error in Figure 5. The image for Figure 5a is identical to Figure 5c. The corrected Figure 5 is shown below.

We apologize for this error.

陈,A. A.,费,s -z。, Lenssen, a.w., &;Moore, K. J.(2022)。光热控制下白豌豆营养休眠。草地研究,1(1),43-52。https://doi.org/10.1002/glr2.12008There在图5中是一个错误。图5a的图像与图5c相同。更正后的图5如下所示。我们为这个错误道歉。
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引用次数: 0
Responses to defoliation and fertiliser, corm development and chemical control of onion grass (Romulea rosea) in the Mediterranean environment of southern Australia 南澳大利亚地中海环境洋葱草(Romulea rosea)对落叶、肥料、球茎发育和化学防治的响应
Pub Date : 2022-10-23 DOI: 10.1002/glr2.12030
Zhongnan Nie, Reto Zollinger, Ralph Behrendt

Background

Onion grass (Romulea rosea) is a common weed that infests native and improved pastures in the Mediterranean environments of southern Australia. It is a very challenging weed to control due to its distinctive growth and survival mechanisms involving corms.

Methods

Three glasshouse experiments were conducted in Australia to investigate the response of onion grass to defoliation and fertiliser application, the development and growth of onion grass corms and the chemical control of the weed in winter and spring.

Results

Defoliating consistently to 1 cm above ground reduced corm weight by 84% compared with the control. Medium or high fertiliser application did not increase herbage mass of onion grass compared with the nonfertilised treatment. The corm weight of onion grass declined from Weeks 1 to 8 after emergence, remained at 53–60 mg dry matter (DM) corm−1 from Weeks 8 to 19 and then declined on average to 37 mg DM corm−1 up to Week 26 after emergence. New corms started to develop at Week 6 after emergence. A small proportion of the onion grass plants (7.5%) developed multiple new corms (3–6 corms) from a single old corm. The most effective herbicide control was a winter application of metsulfuron methyl or imazamox as an alternative product to protect clover.

Conclusions

This study has identified the growth and development patterns of onion grass corms, how the plant responded to defoliation and fertiliser application and effective chemical control of this weed. These findings have significant practical implications for the improvement of onion grass-infested pastures.

洋葱草(Romulea rosea)是一种常见的杂草,常见于澳大利亚南部地中海地区的原生和改良牧场。由于其独特的生长和涉及球茎的生存机制,它是一种非常具有挑战性的杂草。方法在澳大利亚进行3个温室试验,研究冬、春季洋葱草对落叶和施肥的反应、洋葱草球茎的发育和生长以及化学防治措施。结果与对照相比,在离地1cm处持续落叶可使球茎重量减少84%。与不施肥处理相比,中、高施肥处理没有增加洋葱草的牧草质量。出苗后第1 ~ 8周,洋葱草茎重呈下降趋势,第8 ~ 19周保持在53 ~ 60 mg干物质(DM)茎重,到第26周,平均下降至37 mg DM茎重。新球茎在出苗后第6周开始发育。一小部分洋葱草(7.5%)从一个老球茎发育出多个新球茎(3-6个)。最有效的除草剂防治是冬季使用甲基甲磺隆或伊玛唑莫作为保护三叶草的替代产品。结论本研究明确了洋葱草球茎的生长发育规律、对落叶和施肥的反应以及有效的化学防治措施。这些发现对改善洋葱草牧场具有重要的实际意义。
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引用次数: 0
Growth performance of different Epichloë sinensis strains on media with different additives 不同Epichloë sinensis菌株在不同添加剂培养基上的生长性能
Pub Date : 2022-10-18 DOI: 10.1002/glr2.12029
Pei Tian, Meining Wang, Yang Luo, Xingdi Wang, Wenbo Xu

Background

Epichloë sinensis is a newly found endophyte species that is frequently involved in symbiosis with Festuca sinensis. There are a little reports on the characteristics of E. sinensis in vitro.

Methods

In the present study, five E. sinensis endophyte strains (strain ID 1, 2, 41D, 57D, 111D) isolated from different F. sinensis ecotypes were used to evaluate their antioxidant capacities and enzyme activities. Also, the growth of E. sinensis on potato dextrose agar (PDA) with different additives was investigated.

Results

The five strains had significantly different (p < 0.05) total antioxidant capacities and radical-scavenging abilities. In most cases, the growth rate of strain 1 was the fastest (p < 0.05), and that of strain 57D was the slowest (p < 0.05). The growth rates of these five strains on PDA with vitamins significantly (p < 0.05) increased with increasing vitamin concentration. The growth rates of these five strains on PDA with plant growth regulators also significantly (p < 0.05) increased; however, the increase was inconsistent at different concentrations of indole-3-acetic acid and gibberellin. Low concentrations (5 and 10 mg L−1) of forchlorfenuron (KT-30) significantly promoted the growth of all five strains, whereas high concentrations (20 and 40 mg L−1) of KT-30 significantly inhibited their growth (p < 0.05). The Na+, Cd2+, Zn2+, and Cr6+ ions all inhibited the growth of these five strains, and their growth rates were significantly decreased with an increase in ion concentrations (p < 0.05). Under Na+ stress, strain 41D grew the fastest and had the strongest tolerance (p < 0.05); under Cd2+, Zn2+, and Cr6+ stresses, strain 111D grew the fastest and had the strongest tolerance (p < 0.05).

Conclusions

The results indicated that the E. sinensis had antioxidant activities and hydrolase activities. Additives could promote or inhibit the growth of the strains in different degrees. All these evaluations in the present study improve our understanding of the E. sinensis endophyte and provide referenced information for further studies.

Epichloë sinensis是新发现的一种常与羊茅共生的内生植物。目前关于中华棘球绦虫体外特性的报道很少。方法采用不同生态型中华赤霉病菌内生菌株ID 1、2、41D、57D、111D,对其抗氧化能力和酶活性进行研究。同时,研究了不同添加剂对马铃薯葡萄糖琼脂(PDA)上中华赤霉素的生长情况。结果5个菌株的总抗氧化能力和自由基清除能力差异有统计学意义(p < 0.05)。在大多数情况下,菌株1的生长速度最快(p < 0.05),菌株57D的生长速度最慢(p < 0.05)。这5株菌株在添加维生素的PDA上的生长速率随着维生素浓度的增加而显著提高(p < 0.05)。这5个菌株在添加植物生长调节剂的PDA上的生长速率也显著提高(p < 0.05);但不同浓度吲哚-3-乙酸和赤霉素的增加不一致。低浓度(5和10 mg L−1)对5种菌株的生长均有显著促进作用,高浓度(20和40 mg L−1)对5种菌株的生长均有显著抑制作用(p < 0.05)。Na+、Cd2+、Zn2+和Cr6+离子均抑制了这5种菌株的生长,且随着离子浓度的增加,它们的生长速率显著降低(p < 0.05)。在Na+胁迫下,菌株41D生长最快,耐受性最强(p < 0.05);在Cd2+、Zn2+和Cr6+应力下,应变111D生长最快,耐受性最强(p < 0.05)。结论中华棘球蚴具有抗氧化活性和水解酶活性。添加剂对菌株的生长有不同程度的促进和抑制作用。这些评价提高了我们对中华赤杨内生菌的认识,为进一步的研究提供了参考信息。
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引用次数: 0
Trends of seasonal forage yield changes of triticale in the southern Great Plains of the United States 美国大平原南部小黑麦牧草季节性产量变化趋势
Pub Date : 2022-10-13 DOI: 10.1002/glr2.12027
Tadele T. Kumssa, Joshua D. Anderson, James P. Johnson, Shawn Norton, Malay C. Saha, Michael A. Trammell, James K. Rogers, Twain J. Butler, Xue-Feng Ma

Background

The focus of triticale breeding in the southern Great Plains of the United States has been to increase forage yield for autumn–winter seasons when most other forage species are dormant. This study aims to estimate the trends of seasonal yield changes over time.

Methods

Yield trials had been conducted in a randomized complete block design for up to two decades. Seasonal yield changes over time were estimated using linear regression analysis.

Results

The analysis revealed a significant positive relation between autumn forage yield (relative to common checks) and calendar years when the trials were conducted. The estimated improvement of autumn forage yield was about 1.9% per year on medium and heavy soil at Ardmore, Oklahoma, and about 5.4% per year on light soil at Burneyville, Oklahoma. However, winter forage yield change was minimal and the spring forage yield increase was negative, although this decline was not significant. Total forage yield improvements were about 0.6% and 0.7% per year at Ardmore and Burneyville, respectively. Similar results were also observed when years of cultivars' introduction were used instead.

Conclusions

The results indicate a significant gain in autumn forage yield, but a minor gain in the total forage yield due to the yield tradeoff between the autumn and spring seasons.

在美国南部大平原,小黑麦育种的重点是增加秋冬季节的饲料产量,而此时大多数其他牧草都处于休眠状态。本研究旨在估计季节性产量随时间变化的趋势。方法产量试验在随机完全区组设计中进行了长达20年。利用线性回归分析估计季节性产量随时间的变化。结果分析显示,秋季牧草产量(相对于普通检查)与试验进行时的历年呈显著正相关。估计在俄克拉何马州Ardmore的中、重质土壤上,秋季牧草产量每年提高约1.9%,在Burneyville的轻质土壤上,每年提高约5.4%。冬季饲草产量变化不大,春季饲草产量呈负增长,但下降幅度不显著。在Ardmore和Burneyville,牧草总产量每年分别提高0.6%和0.7%。用多年的品种引种来代替,也观察到类似的结果。结论秋季牧草产量显著增加,但由于秋季和春季之间的产量权衡,总牧草产量增加较小。
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Grassland Research
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