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Growth performance of different Epichloë sinensis strains on media with different additives 不同Epichloë sinensis菌株在不同添加剂培养基上的生长性能
Pub Date : 2022-10-18 DOI: 10.1002/glr2.12029
Pei Tian, Meining Wang, Yang Luo, Xingdi Wang, Wenbo Xu

Background

Epichloë sinensis is a newly found endophyte species that is frequently involved in symbiosis with Festuca sinensis. There are a little reports on the characteristics of E. sinensis in vitro.

Methods

In the present study, five E. sinensis endophyte strains (strain ID 1, 2, 41D, 57D, 111D) isolated from different F. sinensis ecotypes were used to evaluate their antioxidant capacities and enzyme activities. Also, the growth of E. sinensis on potato dextrose agar (PDA) with different additives was investigated.

Results

The five strains had significantly different (p < 0.05) total antioxidant capacities and radical-scavenging abilities. In most cases, the growth rate of strain 1 was the fastest (p < 0.05), and that of strain 57D was the slowest (p < 0.05). The growth rates of these five strains on PDA with vitamins significantly (p < 0.05) increased with increasing vitamin concentration. The growth rates of these five strains on PDA with plant growth regulators also significantly (p < 0.05) increased; however, the increase was inconsistent at different concentrations of indole-3-acetic acid and gibberellin. Low concentrations (5 and 10 mg L−1) of forchlorfenuron (KT-30) significantly promoted the growth of all five strains, whereas high concentrations (20 and 40 mg L−1) of KT-30 significantly inhibited their growth (p < 0.05). The Na+, Cd2+, Zn2+, and Cr6+ ions all inhibited the growth of these five strains, and their growth rates were significantly decreased with an increase in ion concentrations (p < 0.05). Under Na+ stress, strain 41D grew the fastest and had the strongest tolerance (p < 0.05); under Cd2+, Zn2+, and Cr6+ stresses, strain 111D grew the fastest and had the strongest tolerance (p < 0.05).

Conclusions

The results indicated that the E. sinensis had antioxidant activities and hydrolase activities. Additives could promote or inhibit the growth of the strains in different degrees. All these evaluations in the present study improve our understanding of the E. sinensis endophyte and provide referenced information for further studies.

Epichloë sinensis是新发现的一种常与羊茅共生的内生植物。目前关于中华棘球绦虫体外特性的报道很少。方法采用不同生态型中华赤霉病菌内生菌株ID 1、2、41D、57D、111D,对其抗氧化能力和酶活性进行研究。同时,研究了不同添加剂对马铃薯葡萄糖琼脂(PDA)上中华赤霉素的生长情况。结果5个菌株的总抗氧化能力和自由基清除能力差异有统计学意义(p < 0.05)。在大多数情况下,菌株1的生长速度最快(p < 0.05),菌株57D的生长速度最慢(p < 0.05)。这5株菌株在添加维生素的PDA上的生长速率随着维生素浓度的增加而显著提高(p < 0.05)。这5个菌株在添加植物生长调节剂的PDA上的生长速率也显著提高(p < 0.05);但不同浓度吲哚-3-乙酸和赤霉素的增加不一致。低浓度(5和10 mg L−1)对5种菌株的生长均有显著促进作用,高浓度(20和40 mg L−1)对5种菌株的生长均有显著抑制作用(p < 0.05)。Na+、Cd2+、Zn2+和Cr6+离子均抑制了这5种菌株的生长,且随着离子浓度的增加,它们的生长速率显著降低(p < 0.05)。在Na+胁迫下,菌株41D生长最快,耐受性最强(p < 0.05);在Cd2+、Zn2+和Cr6+应力下,应变111D生长最快,耐受性最强(p < 0.05)。结论中华棘球蚴具有抗氧化活性和水解酶活性。添加剂对菌株的生长有不同程度的促进和抑制作用。这些评价提高了我们对中华赤杨内生菌的认识,为进一步的研究提供了参考信息。
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引用次数: 0
Trends of seasonal forage yield changes of triticale in the southern Great Plains of the United States 美国大平原南部小黑麦牧草季节性产量变化趋势
Pub Date : 2022-10-13 DOI: 10.1002/glr2.12027
Tadele T. Kumssa, Joshua D. Anderson, James P. Johnson, Shawn Norton, Malay C. Saha, Michael A. Trammell, James K. Rogers, Twain J. Butler, Xue-Feng Ma

Background

The focus of triticale breeding in the southern Great Plains of the United States has been to increase forage yield for autumn–winter seasons when most other forage species are dormant. This study aims to estimate the trends of seasonal yield changes over time.

Methods

Yield trials had been conducted in a randomized complete block design for up to two decades. Seasonal yield changes over time were estimated using linear regression analysis.

Results

The analysis revealed a significant positive relation between autumn forage yield (relative to common checks) and calendar years when the trials were conducted. The estimated improvement of autumn forage yield was about 1.9% per year on medium and heavy soil at Ardmore, Oklahoma, and about 5.4% per year on light soil at Burneyville, Oklahoma. However, winter forage yield change was minimal and the spring forage yield increase was negative, although this decline was not significant. Total forage yield improvements were about 0.6% and 0.7% per year at Ardmore and Burneyville, respectively. Similar results were also observed when years of cultivars' introduction were used instead.

Conclusions

The results indicate a significant gain in autumn forage yield, but a minor gain in the total forage yield due to the yield tradeoff between the autumn and spring seasons.

在美国南部大平原,小黑麦育种的重点是增加秋冬季节的饲料产量,而此时大多数其他牧草都处于休眠状态。本研究旨在估计季节性产量随时间变化的趋势。方法产量试验在随机完全区组设计中进行了长达20年。利用线性回归分析估计季节性产量随时间的变化。结果分析显示,秋季牧草产量(相对于普通检查)与试验进行时的历年呈显著正相关。估计在俄克拉何马州Ardmore的中、重质土壤上,秋季牧草产量每年提高约1.9%,在Burneyville的轻质土壤上,每年提高约5.4%。冬季饲草产量变化不大,春季饲草产量呈负增长,但下降幅度不显著。在Ardmore和Burneyville,牧草总产量每年分别提高0.6%和0.7%。用多年的品种引种来代替,也观察到类似的结果。结论秋季牧草产量显著增加,但由于秋季和春季之间的产量权衡,总牧草产量增加较小。
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引用次数: 0
Forage production and persistence characteristics of grazed native warm-season grass mixtures with or without nitrogen fertilizer 施用或不施用氮肥的本地暖季牧草混合放牧的饲料产量和持久性特征
Pub Date : 2022-10-12 DOI: 10.1002/glr2.12028
Caroline Nicole Chappell, Marty Landon Marks, Katie Michelle Mason, Liliane Severino da Silva, Joshua Luke Jacobs, Mary Kimberly Mullenix, Sandra Leanne Dillard, Russell Brian Muntifering

Background

Native warm-season grass (NWSG) mixtures may provide a low-nitrogen (N)-input summer perennial forage option to extensively managed forage–livestock systems.

Methods

Mixed pastures of big bluestem (Andropogon gerardii Vitman), little bluestem (Schizachyrium scoparium Michx.), and indiangrass (Sorghastrum nutans L.) fertilized with 0 or 67 kg N ha−1 were continuously stocked with beef heifers and cows. Forage mass, nutritive value, and canopy heights were determined every 2 weeks during the grazing season. Stand persistence measures included the canopy cover and leaf area index (LAI) and plant crown density at spring emergence following 3 years of grazing management.

Results

Forage mass, canopy height, and stocking densities were greater for N-fertilized NWSG than unfertilized NWSG for the first 30 days of the growing season across the 3-year study. Forage NWSG fertilized with N had a greater decrease in LAI during the growing season (51% decrease) than unfertilized NWSG. Spring NWSG plant density estimates following 3 years of grazing did not differ across N management strategies.

Conclusions

Forage NWSG mixtures supported superior forage attributes and greater stocking densities early in the grazing season under low-level N than zero-N-input systems and may provide a low-N-input alternative for improved species use in southeastern US forage–livestock systems.

本地暖季草(NWSG)混合物可能为广泛管理的牧草-牲畜系统提供低氮(N)投入的夏季多年生饲料选择。方法采用大蓝茎(Andropogon gerardii Vitman)、小蓝茎(Schizachyrium scoparium Michx.)和印度草(Sorghastrum nutans L.)混合牧场,施肥0或67 kg N ha−1,连续放养肉牛和小母牛。放牧季节每2周测定一次牧草质量、营养价值和冠层高度。林分持久性测量包括3年放牧后林冠盖度、叶面积指数(LAI)和春季出苗期植物冠密度。结果在生长季的前30天,施氮的NWSG的饲料质量、冠层高度和载畜密度均大于未施氮的NWSG。与未施肥的NWSG相比,施氮的NWSG在生长季节的LAI下降幅度更大(下降51%)。放牧3年后的春季NWSG植物密度估计值在不同的氮管理策略下没有差异。结论在低氮条件下,牧草NWSG混合物在放牧季节早期比零氮输入系统具有更好的牧草属性和更高的放养密度,可能为改善美国东南部牧草-牲畜系统的物种利用提供低氮输入的替代方案。
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引用次数: 0
The role of herbivores in the grassland carbon budget for Three-Rivers Headwaters region, Qinghai–Tibetan Plateau, China 青藏高原三江源地区草食动物在草地碳收支中的作用
Pub Date : 2022-10-06 DOI: 10.1002/glr2.12025
Junbang Wang, Xinquan Zhao, Xihuang Ouyang, Liang Zhao, Wenying Wang, Chan Zuo, Zhenhua Zhang, Huakun Zhou, Alan Watson, Yingnian Li

Background

An accurate assessment of the carbon budget is a crucial part of projecting future climate change and its impact on ecosystems. Grasslands foster multiple ecological functions including support for wild animals and livestocks. Herbivores intake forage biomass carbon, then digest and metabolize, and finally retain some carbon. The carbon processes have not been well quantified, resulting in uncertainties in the estimation of regional carbon budgets for grassland ecosystems.

Methods

An animal metabolic carbon flux model was developed for herbivores in the Three-Rivers Headwaters region of China. The forage intake and metabolic carbon rates were estimated through metabolic body weight and daily digested measures for the main herbivore species.

Results

The carbon intake was 5.52 Tg C year−1 (45%) from partial aboveground biomass (12.2 Tg C year−1), in which 39.31% was released into the atmosphere by respiration CO2, 43.77% was returned to the ecosystem as feces and urine, and 16.96% was retained in herbivores for population regeneration or for human well-being.

Conclusions

This study, as the first research on this topic, quantified the carbon flux of herbivores and found livestock accounts for a major part of consumed carbon on grasslands, which is important for understanding regional carbon budgets to mitigate and adapt to climate change over grasslands worldwide.

准确评估碳收支是预测未来气候变化及其对生态系统影响的关键部分。草原具有多种生态功能,包括支持野生动物和牲畜。草食动物摄取牧草生物量碳,然后进行消化代谢,最后保留部分碳。由于碳过程尚未得到很好的量化,导致草地生态系统区域碳收支估算存在不确定性。方法建立三江源地区草食动物代谢碳通量模型。通过代谢体重和日消化量测定主要食草动物的采食量和代谢碳率。结果部分地上生物量(12.2 Tg C)的碳摄入量为5.52 Tg C(45%),其中39.31%通过呼吸释放到大气中,43.77%以粪便和尿液的形式返回生态系统,16.96%保留在食草动物中用于种群更新或人类福祉。结论本研究首次量化了草食动物的碳通量,发现牲畜占草原碳消耗的主要部分,这对于了解全球草原区域碳收支以减缓和适应气候变化具有重要意义。
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引用次数: 0
Understanding sheep baa-haviour: Investigating the relationship between pasture and animal grazing patterns 了解羊的咩咩行为:调查牧场和动物放牧模式之间的关系
Pub Date : 2022-10-06 DOI: 10.1002/glr2.12026
Danica Parnell, Igor Kardailsky, Jacob Parnell, Warwick Brabazon Badgery, Lachlan Ingram

Background

Grasslands are the primary source of feed for grazing livestock, and as such, knowledge on how to best manage livestock and grasslands, through the use of spatiotemporal modelling, will assist in the long-term management of a valuable ecosystem resource.

Methods

This study was conducted over 14 months between March and April 2017 in Orange, NSW, Australia. The study evaluated sheep behaviour in relation to the presence of pasture species, environment and paddock structures, using random forest modelling, to predict sheep location under continuous high (HSR, 13 DSE ha−1) and low (LSR, 7 DSE ha−1) stocking rates.

Results

In the LSR, significant drivers included water, shade and fence lines (p < 0.01). In the HSR, only fence lines and available biomass were found to be significant (p < 0.01). The presence of green legumes in both stocking rates often increased residency by sheep. Animals spent more time together in the LSR, suggesting that social behaviour played a larger role than pasture quantity and quality in driving grazing behaviours.

Conclusions

Understanding how pasture type can influence grazing behaviours and also how animal behaviour affects pasture performance and utilisation is important in developing long-term sustainable management strategies on a paddock scale.

草原是放牧牲畜的主要饲料来源,因此,通过使用时空模型,了解如何最好地管理牲畜和草原,将有助于对宝贵的生态系统资源进行长期管理。方法本研究于2017年3月至4月在澳大利亚新南威尔士州奥兰治进行,为期14个月。该研究利用随机森林模型,评估了羊的行为与牧场物种、环境和围场结构的关系,以预测连续高(高海拔,13平方平方平方公顷−1)和低(低海拔,7平方平方平方公顷−1)放养率下羊的位置。结果在LSR中,水、树荫和栅栏线是显著的驱动因素(p < 0.01)。在高铁地区,只有栅栏线和可利用生物量显著(p < 0.01)。在两种放养率中,绿色豆科植物的存在往往增加了羊的居住。动物在LSR中花更多的时间在一起,这表明社会行为比牧场数量和质量在驱动放牧行为方面发挥更大的作用。了解牧场类型如何影响放牧行为,以及动物行为如何影响牧场性能和利用,对于制定围场规模的长期可持续管理战略具有重要意义。
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引用次数: 2
Quantifying golf course nitrogen use efficiency 量化高尔夫球场氮素利用效率
Pub Date : 2022-10-03 DOI: 10.1002/glr2.12024
Michael A. H. Bekken, Douglas J. Soldat

Background

Previous studies have surveyed golf courses to determine nitrogen (N) fertilizer application rates on golf courses, but no previous studies have attempted to quantify how efficiently golf courses use nitrogen.

Methods

This study tests the ability of the growth potential (GP) N Requirement model as a benchmarking tool to predict a target level of N use on 76 golf courses in 5 regions of the US (Midwest, Northeast, East Texas, Florida, Northwest) and 3 countries in Europe (Denmark, Norway, UK).

Results

The ratio of the golf course-wide N application rate to the GP N requirement prediction (termed the nitrogen efficiency score or NES) was 0.27, indicating that golf courses used 73% less N than predicted by the model. As such, the GP N Requirement model needs to be recalibrated to predict N use on golf courses. This was achieved by adjusting the Nmax coefficient in the model. N rates on golf courses were widely variable both within and across regions. All regions had a coefficient of variation in N rates of 0.46 or greater.

Conclusions

The high variation in N rates, which is largely unexplained by climate, economic factors, grass type, and soil type, may be indicative of inefficient N use in golf course management.

以往的研究已经对高尔夫球场进行了调查,以确定高尔夫球场的氮肥施用量,但此前没有研究试图量化高尔夫球场使用氮肥的效率。方法利用生长潜力(GP) N需求模型作为基准工具,对美国5个地区(中西部、东北部、东德克萨斯州、佛罗里达州、西北部)和欧洲3个国家(丹麦、挪威、英国)的76个高尔夫球场的N利用目标水平进行预测。结果全高尔夫球场氮素施用量与GP氮素需求预测(称为氮效率评分或NES)之比为0.27,表明高尔夫球场的氮素需要量比模型预测少73%。因此,GP N需求模型需要重新校准,以预测高尔夫球场上的N使用。这是通过调整模型中的Nmax系数来实现的。高尔夫球场的氮肥费率在地区内和地区间都有很大差异。各区域氮率变异系数均大于等于0.46。结论气候、经济因素、草地类型和土壤类型可能是导致高尔夫球场氮素利用效率低下的主要原因。
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引用次数: 0
Screening of tall fescue genotypes for relative water content and osmotic potential under drought stress 干旱胁迫下高羊茅相对含水量和渗透势基因型的筛选
Pub Date : 2022-07-01 DOI: 10.1002/glr2.12021
Francis M. Kirigwi, Malay C. Saha

Background

Tall fescue (Festuca arundinacea Schreb.) is an important cool-season perennial grass. Its persistence and forage yield can be severely affected by drought stresses during the hot, dry summers of the southern USA.

Methods

One thousand tall fescue genotypes were evaluated in the greenhouse for high relative water content (RWC) and low cell sap osmotic potential (OP). Fifty contrasting genotypes for the two traits were identified and used in further greenhouse and field studies. These genotypes were also screened with 30% PEG8000. Root and shoot characteristics were studied in 10 genotypes.

Results

The genotypes differed for RWC (33.7%–97.3%, mean: 79.7%) and had an almost fivefold difference in OP (−0.5 to −2.4 MPa, mean: −1.2 MPa). Significant variation (p < 0.001) for the main effects of environment and genotypes was found for RWC and OP. Apart from the greenhouse trial, no correlation was found between RWC and OP, indicating that differences in RWC might have been due to factors other than osmotic adjustment. Genotypes with either long roots or high root weights, and high root/shoot ratios demonstrated high RWC and low OP.

Conclusions

Genotypes with consistently high RWC and low OP were identified and used for the development of mapping populations and transcriptome studies.

高羊茅(Festuca arundinacea Schreb.)是一种重要的寒季多年生牧草。在美国南部炎热干燥的夏季,它的持久性和饲料产量会受到干旱压力的严重影响。方法在温室中对1000株高羊茅进行高相对含水量(RWC)和低细胞液渗透电位(OP)的基因型评价。鉴定了这两个性状的50个对比基因型,并用于进一步的温室和田间研究。这些基因型也用30% PEG8000进行筛选。研究了10个基因型的根、梢性状。结果RWC基因型差异(33.7% ~ 97.3%,平均79.7%),OP基因型差异(- 0.5 ~ - 2.4 MPa,平均- 1.2 MPa)近5倍。环境和基因型对RWC和OP的主要影响有显著差异(p < 0.001)。除温室试验外,RWC和OP之间没有相关性,表明RWC的差异可能是由于渗透调节以外的其他因素造成的。根长或根重高、根/梢比高的基因型表现出高RWC和低OP。结论持续高RWC和低OP的基因型被鉴定出来,并用于定位群体和转录组研究的开发。
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引用次数: 1
Effects of daughter tiller removal on shoot and root growth of the parent tiller in Lolium perenne 除去子蘖对黑麦草茎根生长的影响
Pub Date : 2022-07-01 DOI: 10.1002/glr2.12023
Arif Hasan Khan Robin, Cory Matthew

Background

This study investigated the effects of daughter tiller removal on parent tiller development in Lolium perenne.

Methods

Plants of L. perenne were grown hydroponically to allow separation of roots by phytomer position on the tiller axis and allowed to form two daughter tillers. In Experiment 1, adult daughter tillers were excised and effects on subsequent main tiller growth were observed for 16 days, on average. In Experiment 2, the growth of main tillers with or without daughter tillers was compared over 90–100 days. Two cultivars, ‘Alto’ bred from New Zealand germplasm and ‘Aberdart’ bred from United Kingdom germplasm were tested.

Results

Excision of adult daughter tillers reduced the dry weight of older roots at the base of parent tiller axes and accelerated new phytomer appearance. Preventing tillers from forming daughter tillers by new tiller excision resulted in increased individual leaf and root dry weight in tillers without daughter tillers.

Conclusions

The data indicated that daughter tillers contributed substrate for root development of their parent tillers. The presence of daughter tillers reduced the size of their main tiller but greatly enhanced whole plant yield.

本研究探讨了多年生黑麦草除去子代分蘖对亲本分蘖发育的影响。方法采用水培法,利用分蘖体在分蘖轴上的位置使根分离,形成两个子分蘖。试验1切除成虫分蘖,平均观察16 d后对主分蘖生长的影响。试验2在90 ~ 100 d内比较主分蘖与无子分蘖的生长情况。用新西兰种质资源培育的‘Alto’和英国种质资源培育的‘Aberdart’两个品种进行了试验。结果成年子分蘖的切除减少了母分蘖轴基部老根的干重,加速了新分蘖的形成。通过切除新分蘖防止分蘖形成子分蘖,导致无子分蘖分蘖单叶和根干重增加。结论子代分蘖为其亲本分蘖的根发育提供基质。子分蘖的存在减小了主分蘖的大小,但大大提高了整株产量。
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引用次数: 0
Diverse perennial circular forage systems are needed to foster resilience, ecosystem services, and socioeconomic benefits in agricultural landscapes 需要多样化的多年生循环牧草系统来增强农业景观的恢复力、生态系统服务和社会经济效益
Pub Date : 2022-07-01 DOI: 10.1002/glr2.12020
Valentin D. Picasso, Marisol Berti, Kim Cassida, Sarah Collier, Di Fang, Ann Finan, Margaret Krome, David Hannaway, William Lamp, Andrew W. Stevens, Carol Williams

Prevailing agricultural systems dominated by annual crop monocultures, and the landscapes that contain them, lack resilience and multifunctionality. They are vulnerable to extreme weather events, contribute to degradation of soil, water, and air quality, reduce biodiversity, and negatively impact human health, social engagement, and equity. To achieve greater resilience, stability, and multiple ecosystem services therein, and to improve socioeconomic outcomes, we propose a practical framework to gain multifunctionality at multiple scales. This framework includes forages within agroecosystems that have the essential structural features of diversity, perenniality, and circularity. These three structural features are associated with increased resilience, stability, and provision of several ecosystem services, which in turn improve human health and socioeconomic outcomes. This framework improves understanding of, and access to, tools and materials for promoting the adoption of diverse circular agroecosystems with perennial forages. Application of this framework can result in land transformations that solve sustainability challenges in agriculture if policy, economic, and social barriers can be overcome by a transdisciplinary process of equitable knowledge production.

以一年生作物单一栽培为主的现行农业系统及其景观缺乏适应力和多功能性。它们容易受到极端天气事件的影响,导致土壤、水和空气质量退化,减少生物多样性,并对人类健康、社会参与和公平产生负面影响。为了在其中实现更大的弹性、稳定性和多种生态系统服务,并改善社会经济成果,我们提出了一个实用的框架,以在多个尺度上获得多功能性。该框架包括农业生态系统内具有多样性、多年性和循环性等基本结构特征的牧草。这三个结构特征与增强复原力、稳定性和提供若干生态系统服务有关,从而改善人类健康和社会经济结果。该框架提高了对工具和材料的理解和获取,以促进采用多年生牧草的多种循环农业生态系统。如果能够通过公平的知识生产的跨学科过程克服政策、经济和社会障碍,那么应用这一框架可以导致解决农业可持续性挑战的土地转型。
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引用次数: 3
Defoliation affects the root competitive balance for N between Poa annua plants grown in a split-root box 在分根箱中,剥落影响了黄花蒿根系对氮的竞争平衡
Pub Date : 2022-07-01 DOI: 10.1002/glr2.12022
Sayuki Mori, Louis John Irving

Background

Plants compete aboveground for light and belowground for patchily distributed nutrients. Defoliation causes an immediate loss of leaf area and photosynthetic capacity, leading to reduced root growth, with hypothesised implications for nutrient uptake and nitrogen use efficiency.

Methods

In Experiment 1, we grew single plants in split root boxes with N supplied equally or unequally, with half the plants subject to repeated defoliation to quantify the impact of each treatment. In Experiment 2, we grew pairs of Poa annua plants in three-chamber split root boxes, with N supplied either to the outer chambers (no competition) or to a shared centre chamber (competition), to quantify the influence of defoliation on root competition.

Results

In Experiment 1, defoliation caused a significant decrease in root mass but did not affect root distribution between chambers, while differential N supply led to an 11% increase in shoot mass. In Experiment 2, strong root competition was seen in undefoliated plants under competitive conditions. Where one plant was defoliated, the other exhibited increased shoot mass and N content in competitive, but not noncompetitive conditions.

Conclusions

Our data suggest that plant belowground competitive success following defoliation may be strongly influenced by the spatial distribution of soil resources.

植物在地上争夺光照,在地下争夺斑驳分布的养分。落叶会导致叶面积和光合能力的立即损失,导致根系生长减少,并对养分吸收和氮利用效率产生假设影响。方法在试验1中,我们将单株植物种植在裂根箱中,等量或不等量供氮,其中一半的植株进行重复落叶处理,以量化每种处理的影响。在实验2中,我们在三室裂根箱中种植对对的黄豆植株,分别向外室(无竞争)或向共享的中心室(竞争)提供氮,以量化落叶对根系竞争的影响。结果在试验1中,落叶导致根质量显著降低,但不影响根在室间的分布,而施氮差异导致茎质量增加11%。试验2在竞争条件下,未落叶植物的根系竞争较强。在竞争而非竞争条件下,当一株植物去叶时,另一株的茎部质量和氮含量增加。结论土壤资源的空间分布对植物采叶后的地下竞争成功具有重要影响。
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Grassland Research
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