Infectious Medicine最新文献

{"title":"The growing interests in Epstein–Barr virus: A bibliometric analysis of research trends, collaborations, and emerging hotspots","authors":"Lu Li ,&nbsp;Jialin Wu ,&nbsp;Jianghui Cai ,&nbsp;Muhammad Arif Asghar ,&nbsp;Rui Xiao ,&nbsp;Jingwei Wu ,&nbsp;Qinjian Zhao ,&nbsp;Xiao Zhang","doi":"10.1016/j.imj.2025.100194","DOIUrl":"10.1016/j.imj.2025.100194","url":null,"abstract":"<div><h3>Background</h3><div>Epstein–Barr Virus (EBV) is a widespread human γ-herpesvirus linked to cancers and autoimmune diseases, but limited comprehensive bibliometric analysis appear to have been conducted in this field.</div></div><div><h3>Methods</h3><div>Using Web of Science data, 16,318 EBV-related documents (2014–2023) were analyzed via VOSviewer, Bibliometrix, and Citespace following the Strengthening the Reporting of Observational Studies in Epidemiology reporting guideline.</div></div><div><h3>Results</h3><div>This cross-sectional bibliometric analysis of 16,318 EBV-related documents (2014–2023) revealed a consistent upward trend in annual publications, reflecting growing global interest in EBV research. Collaborative networks demonstrated strong international partnerships, particularly between the United States and China. Keywords co-occurrence and burst analysis highlighted enduring focus on EBV pathogenesis, immune evasion mechanisms, and EBV-associated diseases like nasopharyngeal carcinoma, Hodgkin lymphoma, and multiple sclerosis. On the basis of this comprehensive bibliometric analysis, it showed that the emerging hotspots included immunotherapy, biomarkers, viral reactivation, and vaccine development, with clinical trials evaluating immune-checkpoint inhibitors of toripalimab, mRNA-based therapeutic vaccines targeting LMP2 and EBNA1, and prophylactic strategies such as glycoproteins-based ferritin nanoparticles or mRNA vaccines, indicating a shift toward precision interventions.</div></div><div><h3>Conclusions</h3><div>EBV research has grown exponentially, driven by insights into structural-function relationships and immune evasion. Advances enable targeted prophylactic/therapeutic strategies. The analysis highlights needs to decode virus-host interactions, optimize vaccines, and translate findings clinically, aiming to raise disease awareness, guide immunotherapies, and reduce global health burdens.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 3","pages":"Article 100194"},"PeriodicalIF":0.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative analysis of clinical characteristics between primary and recurrent COVID-19 infections in China","authors":"Yuan Chen ,&nbsp;Qianjin Su ,&nbsp;Dawei Zhang ,&nbsp;Wenting Wei ,&nbsp;Fangfang Zhang ,&nbsp;Qi Li ,&nbsp;Jinxue Zhang","doi":"10.1016/j.imj.2025.100187","DOIUrl":"10.1016/j.imj.2025.100187","url":null,"abstract":"<div><h3>Background</h3><div>Patients with COVID-19 exhibited a variety of clinical characteristics. However, there is currently insufficient evidence to delineate the differences in clinical symptomatology between primary infection and reinfection. This study aims to compare the clinical symptom characteristics between primary infection and reinfection during COVID-19.</div></div><div><h3>Methods</h3><div>This research utilized a convenience sampling method to gather survey data from Chinese individuals aged 18 to 60 years across China. Questionnaire assessments were conducted to collect data on general demographic and clinical information during the COVID-19 pandemic in China. The collected data were analyzed using IBM SPSS 26.0 software.</div></div><div><h3>Results</h3><div>This study analyzed 1156 patients. During second infection, the frequency of fever, painful muscles, ageusia or anosmia, headache, back pain, feeling hot and cold alternately, general tiredness, tingling extremities, heavy arms or legs, and chest pain symptoms were significantly lower. However, the rate of sneezing, runny nose, and stuffy nose were significantly higher (<em>p</em> &lt; 0.05). The proportion of patients with symptoms lasting 3–4 weeks and a body temperature of 38.1–41.0°C was significantly lower during second infection (<em>p</em> &lt; 0.05). The cases infected with COVID-19 for the second time had a higher proportion of nurses and a higher proportion of individuals who received one or two doses of the COVID-19 vaccine (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>The analysis of COVID-19 cases showed significant differences in demographic and clinical symptom characteristics between the first-time and second-time positive populations. This understanding can help guide changes in management strategies.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 3","pages":"Article 100187"},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cytomegalovirus DNAemia detected by next-generation sequencing on short-term prognosis after lung transplantation","authors":"Zhongping Xu ,&nbsp;Yujiao Zhang ,&nbsp;Dapeng Wang,&nbsp;Chenhao Xuan,&nbsp;Zhiyu Li,&nbsp;Hongyang Xu","doi":"10.1016/j.imj.2025.100185","DOIUrl":"10.1016/j.imj.2025.100185","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Cytomegalovirus (CMV) is a common opportunistic pathogen following lung transplantation, associated with post-transplant complications and adverse outcomes. This study aims to evaluate the incidence of CMV DNAemia identified through metagenomic next-generation sequencing (mNGS) during the early postoperative phase of lung transplantation and assess its effects on the short-term outcomes for recipients.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;We conducted a retrospective analysis of clinical data from 115 patients who received lung transplants at the Affiliated Wuxi People's Hospital of Nanjing Medical University between May 2020 and November 2023. Based on mNGS-detected CMV DNAemia status, patients were stratified into DNAemia group and normal group. Nonparametric tests (Mann-Whitney &lt;em&gt;U&lt;/em&gt;/Wilcoxon signed-rank) and mixed-effects models for intergroup comparisons. Kaplan-Meier survival analysis with log-rank testing for overall survival differences. Univariate logistic regression to identify risk factors for ICU mortality and 90-day mortality. Multivariate logistic regression adjusting for confounders. Propensity score matching (1∶1 optimal nearest neighbor, caliper = 0.25) was implemented to address covariate imbalance, followed by univariate logistic regression analyses in the matched cohort.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;In the early postoperative period following lung transplantation, CMV DNAemia was detected via mNGS with an incidence rate of 15.7%. The CMV DNAemia group demonstrated a significantly lower 90-day overall survival rate compared to the normal group, with the Log-rank test revealing statistically significant survival differences between groups (&lt;em&gt;p&lt;/em&gt; &lt; 0.001). Univariate and multivariate logistic regression analyses identified CMV DNAemia as an independent risk factor for ICU all-cause mortality (OR = 5.00, 95% CI: 1.37–18.27, &lt;em&gt;p&lt;/em&gt; = 0.015), while with other pathogens infections independently predicted 90-day all-cause mortality (OR = 3.40, 95% CI: 1.10–10.44, &lt;em&gt;p&lt;/em&gt; = 0.033). After propensity score matching, baseline characteristics were well-balanced between the CMV DNAemia and normal groups. In the matched cohort, univariate logistic regression further confirmed CMV DNAemia as an independent risk factor for ICU mortality (OR = 7.43, 95% CI: 1.23–45.00, &lt;em&gt;p&lt;/em&gt; = 0.029). Mediation analysis demonstrated that co-pathogen infections mediated the relationship between CMV DNAemia and 90-day all-cause mortality, with a proportion mediated of 20.6% (95% CI: 1.7%–138.5%, &lt;em&gt;p&lt;/em&gt; &lt; 0.001).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;mNGS revealed a higher incidence of early CMV DNAemia post-lung transplantation than previously reported. CMV DNAemia significantly correlates with poor prognosis. Despite limitations in sample size and retrospective design, this study provides novel insights into CMV monitoring and management post-transplantation. Future research should determine","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100185"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of glycemic control on lymphocyte subsets in the dissemination of pulmonary tuberculosis: A retrospective analysis","authors":"Yujun Lin ,&nbsp;Xiaohong Chen ,&nbsp;Jiangwei Chen ,&nbsp;Di Wu","doi":"10.1016/j.imj.2025.100183","DOIUrl":"10.1016/j.imj.2025.100183","url":null,"abstract":"<div><h3>Background</h3><div>Extrapulmonary tuberculosis (EPTB) complicates pulmonary tuberculosis (PTB) management. Diabetes mellitus impairs immune function, worsening tuberculosis (TB) outcomes.</div></div><div><h3>Methods</h3><div>This retrospective study investigates the effect of glycemic control on immune function and TB dissemination in 1,768 TB patients (2022–2024). Patients were stratified by glycated hemoglobin (HbA1c) levels (≤ 6% vs. &gt; 6%) and fasting blood glucose (FBG) concentrations (&lt; 7 vs. ≥ 7 mmol/L). Lymphocyte subsets (CD3⁺, CD4⁺, CD8⁺ T cells, CD19⁺ B cells, and CD16⁺CD56⁺ natural killer cells) were compared between glycemic control and TB groups. Multiple regression and threshold effect analysis were conducted to assess the effects of HbA1c and CD3⁺ T cells on TB dissemination and their critical values.</div></div><div><h3>Results</h3><div>Poor glycemic control was associated with lower cell counts of all lymphocyte subsets in patients with PTB (all <em>p</em> &lt; 0.0001). Similar reductions were observed in patients with concurrent PTB and EPTB (PTB + EPTB) when HbA1c values &gt; 6% (all <em>p</em> &lt; 0.05). When HbA1c values ≤ 6% or FBG concentrations &lt; 7 mmol/L, patients with PTB + EPTB showed lower immune cell counts than PTB (<em>p</em> &lt; 0.05). Multiple regression indicated HbA1c increased TB dissemination risk (OR = 10.95), while CD3⁺ T cells showed protective effects. Threshold effect analysis identified an HbA1c values ≥ 7.4% for metabolic control and CD3⁺ T cell thresholds of 387/µL (immune deficiency) and 2,100/µL (immune overactivation).</div></div><div><h3>Conclusions</h3><div>Poor glycemic control impairs immune cells, while EPTB further reduces immune cell numbers. Integrated glycemic management and immunological monitoring help optimize treatment strategies and improve clinical outcomes, particularly in patients at risk for EPTB.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100183"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pneumonia caused by Bordetella hinzii: A case report","authors":"Lei Wang ,&nbsp;Xuan Yao ,&nbsp;Fei He ,&nbsp;Jing Lv ,&nbsp;Haijian Zhou ,&nbsp;Quwen Li","doi":"10.1016/j.imj.2025.100182","DOIUrl":"10.1016/j.imj.2025.100182","url":null,"abstract":"<div><div>As well known, the species of Bordetella, such as <em>Bordetella pertussis</em> and <em>Bordetella parapertussis</em> are prevalent respiratory tract pathogens. <em>Bordetella hinzii</em>, another species of Bordetella genus, <em>Bordetella hinzii</em> could cause meningitis, bacteremia, lung disease, endocarditis, chronic cholangitis and soft tissue abscess. In this study, we reported a new case of <em>Bordetella hinzii</em> infection in Asia with the clinical presentation and laboratory diagnosis. This study systematically analyzed the etiological characteristics of the pathogen using Vitek 2, MALDI-TOF MS, drug susceptibility testing, and whole-genome sequencing, aiming to provide a valuable reference for the diagnosis and treatment of <em>Bordetella hinzii</em> infection<em>.</em></div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100182"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bracing the artificial intelligence technology in viral infectious disease control","authors":"Wei Liu","doi":"10.1016/j.imj.2025.100186","DOIUrl":"10.1016/j.imj.2025.100186","url":null,"abstract":"","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100186"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occupational zoonoses, neurological diseases, and public health: A one health approach","authors":"Angela Stufano ,&nbsp;Valentina Schino ,&nbsp;Domenico Plantone ,&nbsp;Guglielmo Lucchese","doi":"10.1016/j.imj.2025.100184","DOIUrl":"10.1016/j.imj.2025.100184","url":null,"abstract":"<div><div>Zoonotic diseases, which constitute 60% of all human infectious diseases, present substantial risks to public health, economies, and livelihoods. These diseases emerge at the human-animal-environment interface, with occupational exposure representing a critical yet underexamined dimension of zoonotic risk. Workers in high-risk sectors such as agriculture, wildlife management, and laboratory research face elevated exposure to zoonotic pathogens, often under conditions of inadequate preventive measures and resource constraints. Neurological disorders resulting from zoonotic infections, including Guillain-Barré syndrome, encephalitis, and meningitis, illustrate the severe health consequences for occupational groups. Cases linked to swine hepatitis E virus, West Nile virus, <em>Streptococcus suis</em>, and <em>Baylisascaris procyonis</em> underscore the urgent need for robust surveillance and targeted interventions.</div><div>The Ecohealth approach, integrated with the One Health framework, provides a transformative model for managing zoonotic risks by addressing the upstream drivers of disease emergence. By emphasizing environmental stewardship, ecological balance, and socio-economic equity, Ecohealth fosters sustainable preventive strategies. Occupational medicine is crucial in linking workplace safety with public health through tailored risk management, enhanced surveillance, and targeted education.</div><div>Despite these frameworks, significant barriers persist, including data gaps, underreporting of occupational diseases, and insufficient coordination among health sectors. Addressing these challenges requires implementing standardized occupational health surveillance systems, enhancing reporting mechanisms through digital tools, and promoting cross-sectoral data-sharing initiatives. Successful models, such as sentinel surveillance programs in agricultural sectors and integrated biosurveillance networks, demonstrate the feasibility of these strategies. Leveraging these approaches can facilitate early detection, improve reporting accuracy, and support evidence-based interventions.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100184"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of a novel coltivirus from Haemaphysalis concinna ticks in Northeastern China","authors":"Yu-Hong Yang ,&nbsp;Ji-Xu Li ,&nbsp;Rui-Chen Wang ,&nbsp;Qi-Kai Yin ,&nbsp;Shi-Hong Fu ,&nbsp;Kai Nie ,&nbsp;Qian-Qian Cui ,&nbsp;Song-Tao Xu ,&nbsp;Qiang Wei ,&nbsp;Fan Li ,&nbsp;Xing-Zhou Li ,&nbsp;Huan-Yu Wang","doi":"10.1016/j.imj.2025.100179","DOIUrl":"10.1016/j.imj.2025.100179","url":null,"abstract":"<div><h3>Backgroud</h3><div>Coltiviruses are spherical, non-enveloped viruses with 12 double-stranded RNA segments, belonging to the family <em>Spinareoviridae</em>, and predominantly transmitted by ticks. This study isolated and characterized a novel coltivirus, designated Woodland tick reovirus (WLTRV), from <em>Haemaphysalis concinna</em> ticks collected in Helong City, Jilin Province, in Northeastern China.</div></div><div><h3>Methods</h3><div>SW-13 and Vero cells were used to isolate WLTRV through three blind passages, while seven mammalian cell lines assessed viral growth. Viral morphology was observed by electron microscopy. Next-generation sequencing, 5ʹ and 3ʹ rapid amplification of cDNA ends were used to determine WLTRV whole genome sequences, and phylogenetic methods were used to characterize WLTRV. A real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect WLTRV RNA in ticks.</div></div><div><h3>Results</h3><div>WLTRV grew and exerted cytopathic effects in human (SW-13 and 293T) and mouse (BHK-21 and N2A) cell lines, revealing its potential to infect mammals. Phylogenetic analysis based on RNA-dependent RNA polymerase sequences classified WLTRV within the genus <em>Coltivirus</em>, with a close evolutionary relationship to Tarumizu tick virus. The nucleotide and amino acid sequence homologies between WLTRV and Tarumizu tick virus across the 12 segments analyzed ranged from approximately 44.79% to 69.09% and 33.73% to 75.60%, respectively. WLTRV shared conserved the 5ʹ-terminal (GACA^A/_U^U/_A) and 3ʹ-terminal (UGCAGUC) consensus sequences of the genus <em>Coltivirus</em> genomes. Electron microscopy revealed WLTRV as spherical (diameter ∼80 nm), non-enveloped, and morphologically consistent with coltiviruses. Among the 4,717 ticks collected from six towns in the Yanbian Korean Autonomous Prefecture, WLTRV RNA was only detected in <em>H. concinna</em> (0.95% virus-carrying rate) but not in <em>Haemaphysalis japonica, Haemaphysalis longicornis, Ixodes persulcatus</em>, and <em>Dermacentor silvarum</em>.</div></div><div><h3>Conclusions</h3><div>This study represents the first isolation and identification of WLTRV from <em>H. concinna</em> in the Yanbian Korean Autonomous Prefecture, providing new insights into the genetic diversity and evolution of the genus <em>Coltivirus</em>.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100179"},"PeriodicalIF":0.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification","authors":"Xinbei Jia ,&nbsp;Linglong Wan ,&nbsp;Fei Xiao ,&nbsp;Xiaolan Huang ,&nbsp;Juan Zhou ,&nbsp;Yi Wang ,&nbsp;Jun Tai","doi":"10.1016/j.imj.2025.100181","DOIUrl":"10.1016/j.imj.2025.100181","url":null,"abstract":"<div><h3>Background</h3><div>Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) are significant causative agents of acute respiratory tract infections that are prevalent among school-based outbreaks across China. Rapid and accurate diagnosis is crucial for effective control and treatment of HAdV infection.</div></div><div><h3>Methods</h3><div>Here, we developed a novel diagnostic assay combining multiple cross displacement amplification (MCDA) with CRISPR-Cas12b technology, designated HAdV-MCDA-CRISPR, to rapidly detect HAdV-3 and HAdV-7. The assay targets a highly conserved region of the hexon gene, enabling broad detection of these serotypes. The protocol includes DNA extraction (15 minutes), MCDA amplification (40 minutes), and CRISPR detection (5 minutes), and is completed within one hour. Specificity was validated by testing against non-HAdV pathogens, while sensitivity was assessed using serial dilutions of hexon-containing plasmid DNA. Clinical performance was evaluated using 88 patient samples.</div></div><div><h3>Results</h3><div>The HAdV-MCDA-CRISPR assay demonstrated high sensitivity, detecting as little as 5 fg HAdV plasmid DNA per reaction, and showed no cross-reactivity with other common respiratory pathogens. Clinical validation using 88 patient samples further demonstrated the diagnostic accuracy of HAdV-MCDA-CRISPR.</div></div><div><h3>Conclusions</h3><div>HAdV-MCDA-CRISPR is a rapid, sensitive, and specific tool for diagnosing HAdV-3 and HAdV-7 infections, offering potential for timely clinical intervention and enhanced epidemiological surveillance.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100181"},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report","authors":"Yuanchao Xue,&nbsp;Filipe M. Cerqueira,&nbsp;Heather L. Stevenson,&nbsp;Natalie Williams-Bouyer,&nbsp;Rong Fang","doi":"10.1016/j.imj.2025.100180","DOIUrl":"10.1016/j.imj.2025.100180","url":null,"abstract":"<div><div>Pyogenic liver abscess (PLA) is a potentially life-threatening disease. Early diagnosis and appropriate treatment are crucial to ensure high-quality healthcare for patients with PLA. However, this is complicated by their non-specific clinical symptoms. In addition, the etiologic organisms responsible for PLA are frequently culture-negative, thus complicating clinical decision-making. Here, we report a case of PLA caused by <em>Streptococcus intermedius,</em> as identified via DNA metagenomic sequencing of plasma.</div></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"4 2","pages":"Article 100180"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}