Journal of Holistic Integrative Pharmacy最新文献_第8页

Exploring the mechanism of Lingbao Huxin Dan in the treatment of bradycardia based on atrioventricular node electrical signal conduction 基于房室结电信号传导探讨灵宝护心丹治疗心动过缓的作用机制

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 Epub Date: 2024-12-24 DOI: 10.1016/j.jhip.2024.12.003

Jing Zhang , Guobin Zheng , Shangjing Liu , Yaodong Miao , Shu Yang , Sheng Li , Zhihui Yang , Danping Zhuo , Rui Guo , Yang Guo , Rongjiang Shao , Yunqing Hua , Chuanrui Ma

Background

Prolonged bradycardia leads to inadequate pumping of the heart, causing myocardial ischemia, which in turn affects cardiac function. Depending on its clinical features, improving the AV node conduction block may be an important tool to inhibit the progression of the disease. Lingbao Huxin Dan, which consists of several traditional Chinese medicines, is used for conditions similar to bradycardia and may be a potential therapeutic agent for bradycardia. In this experiment, we investigated its therapeutic significance and possible therapeutic targets for sinus bradycardia.

Methods

The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. At the end of treatment, the rats' ECG was recorded using a PV-LOOP heart rate recorder. The hearts and serum samples were collected to detect heart rate and related gene expression which were screened through network pharmacology analysis, as well as to evaluate the effect of Lingbao Huxin Dan on ion channels in the cardiac conduction system.

Results

Lingbao Huxin Dan improved the heart rate in the bradycardia model of SD rats and inhibited AV node conduction block by targeting ion channel proteins in the cardiac conduction system. The underlying molecular mechanism may be the activation of the β-adrenergic receptor-dependent cAMP/PKA signaling pathway targeted to enhance the expression of the pacing channel HCN4. In addition, Lingbao Huxin Dan prevented the deterioration of bradycardia by promoting the production of the oxygen radical scavenger SOD and inhibiting the upregulation of LDH due to cardiomyocyte damage.

Conclusions

Our study proved that Lingbao Huxin Dan alleviated bradycardia by downregulating CX45 and enhancing the expression of HCN4, ADRβ1, and TRPM7 to shorten the atrioventricular node induction period and improve sinoatrial node signaling. In addition, Lingbao Huxin Dan relieved myocardial injury induced by bradycardia by reducing the level of oxygen free radicals in the cardiac microenvironment.

背景：长期的心动过缓导致心脏供血不足，引起心肌缺血，进而影响心功能。根据其临床特点，改善房室结传导阻滞可能是抑制疾病进展的重要工具。灵宝护心丹由几种中药组成，用于治疗类似心动过缓的病症，可能是治疗心动过缓的潜在药物。本实验探讨其治疗窦性心动过缓的意义及可能的治疗靶点。方法分别用乙酰胆碱、心得安、维拉帕米制备SD大鼠心动过缓模型，并用灵宝护心丹治疗3周。在治疗结束时，使用PV-LOOP心率记录仪记录大鼠的心电图。采集心脏及血清样本，通过网络药理学分析筛选心率及相关基因表达，评价灵宝护心丹对心脏传导系统离子通道的影响。结果灵宝护心丹可提高SD大鼠心动过缓模型的心率，并通过靶向心脏传导系统的离子通道蛋白抑制房室结传导阻滞。其潜在的分子机制可能是激活β-肾上腺素能受体依赖的cAMP/PKA信号通路，以增强起搏通道HCN4的表达。此外，灵宝护心丹通过促进氧自由基清除剂SOD的产生，抑制心肌细胞损伤引起的LDH上调，从而防止心动过缓的恶化。结论灵宝护心丹通过下调CX45，提高HCN4、ADRβ1、TRPM7的表达，缩短房室结诱导期，改善窦房结信号传导，减轻心动过缓。此外，灵宝护心丹可通过降低心脏微环境中氧自由基水平减轻心动过缓所致心肌损伤。

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引用次数: 0

Network pharmacology-based study on the mechanism of Yiwei Decoction in chronic atrophic gastritis and experimental assessment 基于网络药理学的益胃汤治疗慢性萎缩性胃炎作用机制研究及实验评价

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-12-01 Epub Date: 2024-12-17 DOI: 10.1016/j.jhip.2024.11.004

Zepeng Zhang , Ju Liu , Yi Wang , Xiwen Li , Manman Guo , Menglei Ding , Tongtong Zhu , Lei Zhang

Objective

Yiwei Decoction (YWD) is an ancient TCM formula with historical use in treating chronic atrophic gastritis (CAG). However, its pharmacodynamic mechanisms remain poorly understood. This study aims to study the effect of YWD on CAG through network pharmacology and experimental verification.

Methods

UPLC/MS and the SwissADME database were employed to identify active compounds in YWD. YWD targets were screened by SwissTargetPrediction databases, CAG targets were screened by databases, and the two were intersected for PPI network analysis. The target prediction was performed by GO and KEGG analysis. H₂O₂-induced injury models were established in zebrafish and GES-1 cells, which were subsequently treated with varying doses of YWD. Oxidative stress indicators and apoptosis and inflammation levels in zebrafish and GES-1 cells were assessed using fluorescence microscopy, flow cytometry, and microplate reader assays. The binding capabilities of the core components and core targets were examined using molecular docking. Q-PCR and Western blot were employed to analyze the expression levels of Nrf2, Keap1, and HO-1, respectively.

Results

Forty-nine compounds were identified from YWD. Network pharmacological analysis suggested that YWD may treat CAG by modulating redox-related signaling pathways, inhibiting apoptosis, and reducing inflammation. Subsequent in vitro and in vivo experiments validated these predictions. YWD effectively mitigated H₂O₂-induced oxidative stress in zebrafish and GES-1 cells, suppressing ROS and decreasing apoptosis. YWD reduced inflammation in gastric epithelial cells. Molecular docking results suggested that methylophiopogonanone A and imperatorin may play a key role in the treatment of YWD. Mechanistically, YWD activated the Nrf2 signaling pathway, downregulated Keap1 expression, and upregulated HO-1 and Bcl2 expression.

Conclusion

YWD could improve oxidative stress indicators, inhibiting cell apoptosis, reducing inflammation, and its molecular mechanism of the CAG treatment may be through the Keap1/Nrf2/HO-1 pathways, and to promote the body's antioxidant system.

目的益胃汤是治疗慢性萎缩性胃炎（CAG）的古方。然而，其药效学机制仍然知之甚少。本研究旨在通过网络药理学和实验验证的方法，研究YWD对CAG的影响。方法采用suplc /MS和SwissADME数据库对黄芩中有效成分进行鉴定。YWD靶点通过SwissTargetPrediction数据库筛选，CAG靶点通过数据库筛选，并将两者相交进行PPI网络分析。采用GO和KEGG分析进行靶区预测。在斑马鱼和GES-1细胞中建立h2o2诱导的损伤模型，随后用不同剂量的YWD处理。利用荧光显微镜、流式细胞术和微孔板阅读器检测斑马鱼和GES-1细胞的氧化应激指标、凋亡和炎症水平。利用分子对接技术检测核心组分与核心靶点的结合能力。采用Q-PCR和Western blot分别分析Nrf2、Keap1和HO-1的表达水平。结果从黄芪中鉴定出49个化合物。网络药理学分析表明，YWD可能通过调节氧化还原相关信号通路、抑制细胞凋亡、减轻炎症来治疗CAG。随后的体外和体内实验验证了这些预测。YWD可有效减轻h2o2诱导的斑马鱼和GES-1细胞氧化应激，抑制ROS，减少细胞凋亡。YWD减轻了胃上皮细胞的炎症。分子对接结果提示，甲基参黄酮A和欧前胡素可能在治疗青光眼中起关键作用。机制上，YWD激活Nrf2信号通路，下调Keap1表达，上调HO-1和Bcl2表达。结论ywd能改善氧化应激指标，抑制细胞凋亡，减轻炎症，其治疗CAG的分子机制可能是通过Keap1/Nrf2/HO-1通路，促进机体抗氧化系统。

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引用次数: 0

Ferroptosis: A prospective therapeutic target for radiotherapy- and chemotherapy-induced gastrointestinal inflammation 铁蛋白沉积：放疗和化疗引发的胃肠道炎症的前瞻性治疗靶点

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-08-13 DOI: 10.1016/j.jhip.2024.08.001

Siyu Han, Jingrui Zheng, Weijian Chen, Ke Nie

Ferroptosis is a unique mode of cell death driven by iron-dependent lipid peroxidation, and the process is regulated by a variety of cellular metabolic pathways, including redox homeostasis, iron processing, and lipid metabolism. It has been shown that radiotherapy- and chemotherapy-induced gastrointestinal (GI) inflammation exhibits the key features of ferroptosis, including iron deposition, glutathione (GSH) depletion, glutathione peroxidase 4 (GPX4) inactivation and lipid peroxidation. In this paper, we found that ferroptosis plays an important role in radiotherapy- and chemotherapy-induced GI inflammation, and that elevating GSH levels, activating GPX4, inhibiting elevated levels of lipid peroxidation, and maintaining iron homeostasis significantly alleviated radiotherapy- and chemotherapy-induced GI inflammation. This suggests that ferroptosis may be a new target for the treatment of GI inflammation. In addition, we systematically summarize the potential mechanisms of traditional Chinese medicine (TCM) and its active ingredients in the treatment of GI inflammation, which may be effective in ameliorating radiotherapy- and chemotherapy-induced GI by acting on the key signaling pathways and mediators, such as Nrf2/HO-1, GSH/GPX4, polyunsaturated fatty acids (PUFAs), iron, and organic peroxides, which in turn inhibit the process of ferroptosis, and thereby effectively ameliorate the radiotherapy- and chemotherapy-induced GI inflammation. This finding provides a new potential approach for the treatment of such GI inflammation and demonstrates the potential value of TCM in modern medical treatment.

铁变态反应是由铁依赖性脂质过氧化驱动的一种独特的细胞死亡模式，该过程受多种细胞代谢途径的调控，包括氧化还原平衡、铁处理和脂质代谢。有研究表明，放疗和化疗引起的胃肠道（GI）炎症表现出铁质氧化的主要特征，包括铁沉积、谷胱甘肽（GSH）耗竭、谷胱甘肽过氧化物酶 4（GPX4）失活和脂质过氧化。在本文中，我们发现铁变态反应在放疗和化疗诱发的消化道炎症中起着重要作用，而提高谷胱甘肽水平、激活 GPX4、抑制脂质过氧化水平的升高以及维持铁的稳态能显著缓解放疗和化疗诱发的消化道炎症。这表明，铁氧化可能是治疗消化道炎症的一个新靶点。此外，我们还系统地总结了中药及其有效成分在治疗消化道炎症中的潜在机制，中药可能通过作用于关键信号通路和介质，有效改善放疗和化疗诱导的消化道炎症、Nrf2/HO-1、GSH/GPX4、多不饱和脂肪酸（PUFAs）、铁和有机过氧化物等关键信号通路和介质，进而抑制铁氧化过程，从而有效改善放疗和化疗引起的消化道炎症。这一发现为治疗此类消化道炎症提供了一种新的潜在方法，并证明了中医药在现代医学治疗中的潜在价值。

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引用次数: 0

Research progress in application of alginate gel as tumor drug delivery carrier, for tumor localization and 3D tumor cell model 藻酸盐凝胶作为肿瘤给药载体在肿瘤定位和三维肿瘤细胞模型中的应用研究进展

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-09-11 DOI: 10.1016/j.jhip.2024.08.003

Lili Huang , Yicong Lei , Yucheng Chen , Xin Hu , Chengyu Huang , Huaqing Lin

As one of the hot materials in biomedical research, alginate gel shows great potential in tumor therapy with its unique physical and chemical properties. In order to better meet the complex needs of cancer treatment, alginate brine gel composite system has come into being. This system not only inherits many advantages of single alginate brine gel, but also significantly improves the mechanical properties of the material through compounding, and effectively overcomes the limitations of application. These composites have been applied to drug delivery carriers, tumor targeting systems and three-dimensional tumor cell models in various forms, showing a wide range of application prospects. This paper aims to review the basic structure and properties of alginate gel and analyze the research progress of alginate gel composite systems in the field of cancer in recent years, to provide a valuable reference for the further expansion of the application of this material in tumor therapy.

作为生物医学研究的热点材料之一，海藻酸盐凝胶以其独特的物理和化学性质在肿瘤治疗中显示出巨大的潜力。为了更好地满足肿瘤治疗的复杂需求，海藻酸盐凝胶复合体系应运而生。该体系不仅继承了单一海藻酸盐凝胶的诸多优点，还通过复合显著提高了材料的机械性能，有效克服了应用的局限性。这些复合材料已以多种形式应用于药物输送载体、肿瘤靶向系统和三维肿瘤细胞模型等领域，展现出广泛的应用前景。本文旨在回顾海藻酸凝胶的基本结构和性能，分析近年来海藻酸凝胶复合材料体系在肿瘤领域的研究进展，为进一步拓展该材料在肿瘤治疗中的应用提供有价值的参考。

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引用次数: 0

The research advance on the theory of Chinese medicine-exterior-interior correlation between the Lung and Large Intestine in the treatment of chronic cough 肺与大肠相表里的中医理论在慢性咳嗽治疗中的研究进展

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-09-19 DOI: 10.1016/j.jhip.2024.09.004

Meng Yuan , Hongyuan Chen , Wen Rui

Coughing as the main symptom of a chronic cough is a persistent respiratory disease that poses a substantial challenge to Western medicine and traditional Chinese medicine (TCM). The Large Intestine and Lung are intimately related, impacting each other's pathology and physiology, according to TCM theory. This complex interaction emphasizes how crucial it is to treat both organs at the same time while treating cough to support gut health. This viewpoint is further supported by current research, which demonstrates the connections between the Large Intestine and the Lung through a variety of pathways, including neurological pathways, mucosal immune system modulation, gas exchange dynamics, and microbiota composition. To clarify the mechanisms and practical applications of treating chronic cough by concurrently targeting the Lung and Intestine in TCM, this paper merges ideas from TCM theory and empirical research in Western medicine. This provides insightful information for efficiently managing chronic cough.

以咳嗽为主要症状的慢性咳嗽是一种顽固性呼吸道疾病，对西医和中医都是一个巨大的挑战。中医理论认为，大肠与肺关系密切，在病理和生理上相互影响。这种复杂的相互作用强调了在治疗咳嗽的同时治疗这两个器官以支持肠道健康的重要性。目前的研究进一步支持了这一观点，研究表明大肠与肺之间通过神经通路、粘膜免疫系统调节、气体交换动力学和微生物群组成等多种途径存在联系。为了阐明中医 "肺肠同治 "治疗慢性咳嗽的机制和实际应用，本文融合了中医理论和西医实证研究的观点。这为有效治疗慢性咳嗽提供了具有洞察力的信息。

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引用次数: 0

Exercise therapy: Anti-tumor and improving chemotherapy efficacy 运动疗法：抗肿瘤和提高化疗疗效

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-09-14 DOI: 10.1016/j.jhip.2024.09.001

Zhongyu Wang , Zongming Wang , Huitong Chen , Siyuan Li , Junhua Yang , Yuxin Ma , Chang Zhou , Xiaobao Jin , Jing Liu , Xin Wang

The extant research evidence indicates that sensible exercise has a beneficial impact on the prevention and treatment of cancer. This paper presents a comprehensive literature review of the field of sports oncology. Its objective is to synthesize and analyze the impact of exercise training on cancer, as well as to elucidate the mechanisms through which exercise affects cancer progression. Additionally, it offers valuable insights for advancing fundamental and clinical research in sports oncology. Firstly, this paper provides a summary of the relationship between exercise and various aspects of tumor progression, including tumor size, weight, metastasis, tumor vascularity, myokine production, immune response, and the efficacy of cancer chemotherapy. Secondly, due to the diversity of tumor properties, we also explore the fact that the specificity of exercise prescription should be tailored to the different tumor types and patient profiles. Furthermore, we discuss the importance of considering individual differences when determining the type of exercise, intensity, intervention, and duration of exercise. Finally, this paper emphasizes the necessity of evaluating the interaction between exercise and conventional or novel immunotherapies and pharmacodynamics in future preclinical studies.

现有研究证据表明，合理的运动对预防和治疗癌症有益。本文对运动肿瘤学领域进行了全面的文献综述。其目的是综合分析运动训练对癌症的影响，并阐明运动影响癌症进展的机制。此外，本文还为推进运动肿瘤学的基础和临床研究提供了有价值的见解。首先，本文总结了运动与肿瘤进展各方面的关系，包括肿瘤大小、重量、转移、肿瘤血管、肌肽分泌、免疫反应和肿瘤化疗的疗效。其次，由于肿瘤特性的多样性，我们还探讨了运动处方的特异性应针对不同的肿瘤类型和患者情况。此外，我们还讨论了在确定运动类型、强度、干预措施和运动持续时间时考虑个体差异的重要性。最后，本文强调了在未来临床前研究中评估运动与传统或新型免疫疗法和药效学之间相互作用的必要性。

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引用次数: 0

Preparation of purine functionalized biochar and analysis of nephrotoxic substances in traditional Chinese medicine 嘌呤功能化生物炭的制备及中药中肾毒性物质的分析

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-07-23 DOI: 10.1016/j.jhip.2024.06.006

Yanhui Ge , Xinya Xu , Yuanru Zheng

In recent years, a growing lack of comprehension regarding the safety of traditional Chinese medicines (TCMs) has led to an escalating incidence of drug-induced organ damage, particularly kidney damage associated with TCM usage. This study focused on the development of purine-functionalized biochar and used to capture nephrotoxic substances in TCMs. The biochar was meticulously characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and elemental analysis. Subsequently, it was employed as a solid-phase extraction medium for capturing suspected nephrotoxic substances in TCMs. Results revealed that the functional biochar exhibited commendable selectivity for compounds with nephrotoxic effects, demonstrating its potential for effectively capturing nephrotoxic substances in TCMs. This research aimed to introduce an innovative method for monitoring potential nephrotoxic substances in large-scale TCMs, thereby contributing to the enhancement of the overall safety profile of traditional Chinese medicine.

近年来，由于对传统中药安全性的认识不足，药物引起的器官损伤，尤其是中药引起的肾脏损伤的发病率不断上升。本研究的重点是开发嘌呤功能化生物炭，用于捕捉中药中的肾毒性物质。利用扫描电子显微镜（SEM）、傅立叶变换红外光谱（FT-IR）、X 射线光电子能谱（XPS）和元素分析对生物炭进行了细致的表征。随后，将其用作固相萃取介质，以捕获中药中的可疑肾毒性物质。结果表明，功能性生物炭对具有肾毒性作用的化合物具有良好的选择性，表明其具有有效捕获中药中肾毒性物质的潜力。这项研究旨在引入一种创新方法来监测大型中药中潜在的肾毒性物质，从而有助于提高传统中药的整体安全性。

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引用次数: 0

Safety and risk control study of antibody preparation based on CiteSpace 基于 CiteSpace 的抗体制备的安全性和风险控制研究

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-09-24 DOI: 10.1016/j.jhip.2024.08.002

Chanyuan Chen , Rong Wang , Yuanxuan Cai , Yuhang Zhao , Zherui Chen , Ke Li , Li Zhao , Rui Huang , Nooruldeen Riyadh Ibrahim , Xiaofang Shangguan

Objective

To analyze the hotspots, patterns, and distribution of research on the safety and risk of antibody preparations in the past 20 years. It also seeks to summarize the current status and trends of research on the safety and risk control of antibody preparations.

Methods

Taking “antibody preparation”, “safety” and “risk” as keywords, relevant articles were searched in the databases Web of Science. CiteSpace was utilized to analyze the annual number of publications, countries, authors, institutions, highly cited literature and keywords of the screened literature, and the relevant maps were drawn and the results were analyzed.

Results

A total of 1693 articles were included. The annual number of publications in the field of antibody preparation safety has shown stable growth followed by a rapid increase between 2002 and 2022. Among the countries, the United States accounted for 36.7% of the total publications, ranking first in the world. Large foreign pharmaceutical companies, research institutions, comprehensive university and their affiliated hospitals were the most high-yield institutions. Current hot topics in the field of antibody preparation safety research include “ADC”, “monoclonal antibody”, “anti-tumor activity”, “immunotherapy”, etc.

Conclusion

Over the past 20 years, research on antibody formulations has garnered increasing attention both domestically and internationally, with a focus mainly on efficacy and safety. There has been relatively little research on risk control. In the future, more in-depth research is needed on the mechanisms of adverse reactions in antibody formulations to provide more effective strategies for risk control.

目的分析近 20 年来抗体制剂安全性和风险研究的热点、模式和分布情况，总结抗体制剂安全性和风险控制研究的现状和趋势。方法以 "抗体制剂"、"安全性 "和 "风险 "为关键词，在 Web of Science 数据库中检索相关文章。结果共收录 1693 篇文章。2002-2022年间，抗体制备安全性领域的年发文量呈现出先稳定增长后快速增长的态势。其中，美国的论文数量占论文总数的 36.7%，居世界首位。国外大型制药公司、研究机构、综合性大学及其附属医院是高产机构。目前抗体制剂安全性研究领域的热点话题包括 "ADC"、"单克隆抗体"、"抗肿瘤活性"、"免疫治疗 "等。对风险控制的研究相对较少。未来，需要对抗体制剂的不良反应机制进行更深入的研究，以提供更有效的风险控制策略。

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引用次数: 0

Holistic integrative medicine declaration 整体综合医学宣言

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-08-07 DOI: 10.1016/j.jhip.2024.07.001

China Institute for Development Strategy of Holistic Integrative Medicine

Holistic integrative medicine, abbreviated as HIM, has been officially proposed since 2012. Its theoretical system has been continuously improved, and its practical methods have become increasingly diverse, becoming an inevitable choice and path for the medical development in the new era. This article demonstrates ten major propositions for HIM, elaborating on the connotation and extension of HIM from the perspectives of epistemology and methodology, in order to achieve the transformation and adaptive evolution of modern medicine.

整体整合医学，简称HIM，自2012年正式提出。其理论体系不断完善，实践方法日益多样，成为新时期医学发展的必然选择和必由之路。本文论证了HIM的十大命题，从认识论和方法论的角度阐述了HIM的内涵和外延，以实现现代医学的转型和适应性进化。

引用次数: 0

Puerarin alleviates LPS-induced endothelial cells injury via SIRT1-mediated mitochondrial homeostasis signaling 葛根素通过 SIRT1 介导的线粒体平衡信号缓解 LPS 诱导的内皮细胞损伤

Journal of Holistic Integrative Pharmacy

Pub Date : 2024-09-01 Epub Date: 2024-09-23 DOI: 10.1016/j.jhip.2024.08.004

Xing Chang , Meng Cheng , Ying Li , Xiuteng Zhou

Endothelial inflammation injury is a key mechanism that occurs in the pathological processes of various cardiovascular diseases. Puerarin (PUE) is an isoflavone compound with strong antioxidant properties and the main active component isolated from the rhizome of Pueraria lobata. PUE exhibits a good anti-atherosclerotic pharmacological effect, but there are few studies on the mechanism of its protective effect on endothelial cells. This study found that PUE could regulate to some extent the mitochondrial function of human umbilical vein endothelial cells (HUVECs) and reduce or inhibit lipopolysaccharide-induced inflammatory reactions and oxidative stress injury in HUVECs. Furthermore, the protective effect of PUE on HUVECs was closely related to the SIRT-1 signaling pathway. PUE increased the level of mitophagy and the activity of mitochondrial antioxidant enzymes by increasing SIRT-1 expression, reducing excessive production of ROS, and inhibiting expression of inflammatory factors and oxidative stress injury. Therefore, PUE may improve mitochondrial respiratory function and energy metabolism and increase the activity of HUVECs in the inflammatory state.

内皮炎症损伤是各种心血管疾病病理过程中的一个关键机制。葛根素（PUE）是从葛根根茎中分离出来的主要活性成分，是一种具有很强抗氧化性的异黄酮化合物。葛根素具有良好的抗动脉粥样硬化药理作用，但有关其对血管内皮细胞保护作用机制的研究却很少。本研究发现，葛根素能在一定程度上调节人脐静脉内皮细胞（HUVECs）线粒体功能，减轻或抑制脂多糖诱导的 HUVECs 炎症反应和氧化应激损伤。此外，PUE 对 HUVECs 的保护作用与 SIRT-1 信号通路密切相关。PUE通过增加SIRT-1的表达，减少ROS的过度产生，抑制炎症因子的表达和氧化应激损伤，从而提高线粒体吞噬水平和线粒体抗氧化酶的活性。因此，PUE 可改善线粒体呼吸功能和能量代谢，提高炎症状态下 HUVEC 的活性。

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引用次数: 0