Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie最新文献

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Studies on the Mechanism of Specific Immunological Unresponsiveness 特异性免疫无应答机制的研究

Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie

Pub Date : 1976-03-01 DOI: 10.1016/S0300-872X(76)80047-9

Vesna Tomažič , B. Vitale

The immunological capacity of lymphoid cells from mice rendered tolerant to high and low doses of BSA was investigated. The tolerance was induced by multiple injections of high and low doses of antigen through the period of 30 days. Lymph node and bone marrow cells from tolerant animals were transferred into lethally irradiated syngeneic recipients. After 10 days, when lymphoid organs of the recipients were repopulated with the injected cells, challenge injection of the same antigen incorporated into complete Freund's adjuvant was given. The immune response of the transferred cells in the recipients was evaluated by analysis of the specific antibodies in the sera. Lymphoid cells from donors rendered tolerant with high doses of antigen recovered their reactivity 20 days after the transfer to the level of reaction of normal cells. Lymphoid cells from donors receiving multiple injection of low doses of BSA remained tolerant after the transfer through the entire observation period. According to the cellular events in the donors during the period of tolerance induction, and the behaviour of the transferred lymphoid cells in the new recipients, it seems possible that tolerance induced with high doses of BSA corresponded to the B-cell tolerance, while low doses of antigen most likely induced tolerance of T-cell population. The possible cellular mechanisms of B and T-cell tolerance were discussed.

研究了小鼠淋巴样细胞对高、低剂量牛血清白蛋白耐受的免疫能力。在30天的时间内，通过多次注射高剂量和低剂量抗原诱导耐受。将耐受动物的淋巴结和骨髓细胞转移到受致死性辐射的同基因受体中。10天后，当受体的淋巴器官被注射的细胞重新填充时，将相同的抗原加入完全的弗氏佐剂进行攻毒注射。通过血清中特异性抗体的分析来评价受体转移细胞的免疫反应。来自供体的淋巴细胞对高剂量抗原具有耐受性，在转移到正常细胞的反应水平20天后恢复了它们的反应性。在整个观察期内，接受多次低剂量BSA注射的供体淋巴细胞在转移后仍保持耐受性。根据耐受诱导期间供体的细胞事件和新受体中转移淋巴样细胞的行为，高剂量BSA诱导的耐受似乎与b细胞耐受相对应，而低剂量抗原诱导的t细胞耐受性最有可能。讨论了B细胞和t细胞耐受的可能细胞机制。

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引用次数: 0

A Histoautoradiographic Study of the Localization of Antigen and Specific Antibodies in the Rabbit Spleen 兔脾脏抗原和特异性抗体定位的组织放射自显影研究

Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie

Pub Date : 1976-03-01 DOI: 10.1016/S0300-872X(76)80049-2

L. Rodák

The localization of antigen (chicken serum albumin) and specific antibodies in sections from the spleens of rabbits after primary and secondary immunization was studied by means of histoautoradiography.

After primary immunization, both antigen and antibodies were demonstrated in the lymphoid follicles consistently between 3 and 42 days after injection. Antigen was detected in addition in the marginal zone on the 5th day, and antibodyforming cells in the marginal zone and periarteriolar lymphocyte sheaths between 3 and 9 days after immunization.

After secondary immunization, follicular antigen trapping was detected as early as 2 hours after injection, reaching a peak in the follicles and marginal zone on the 3rd day after immunization. At later periods of time the presence of antigen in the follicles was less prominent than that of specific antibodies. The numbers of antibody-forming cells were 10–100-fold higher in the secondary than in the primary response.

The findings are discussed in relation to the role of antigen-antibody complex in the lymphoid follicles in the development of the capacity to produce the secondary type of immune response.

采用组织放射自显影法研究了兔脾一次免疫和二次免疫后抗原(鸡血清白蛋白)和特异性抗体的定位。初次免疫后，抗原和抗体在注射后3至42天在淋巴滤泡中表现一致。免疫后第5天在边缘区检测到抗原，免疫后第3 ~ 9天在边缘区和小动脉周围淋巴细胞鞘检测到抗体形成细胞。二次免疫后，早在注射后2小时即检测到滤泡抗原捕获，免疫后第3天在卵泡和边缘区达到高峰。在较晚的时期，卵泡中抗原的存在不像特异性抗体那么突出。在继发性反应中，抗体形成细胞的数量比原发性反应高10 - 100倍。研究结果讨论了抗原抗体复合物在淋巴滤泡中产生次级免疫反应能力的作用。

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引用次数: 0

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