Pub Date : 2019-12-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.12.001
Junlan Chuan, T. Xie, Ganggang Wang, Zheng Rong Yang
Objective: To construct a prokaryotic expression plasmid for the large extracellular loop (LEL) of human TSPAN12 protein and obtain highly purified soluble recombinant proteins. Methods: In this experimental study, the coding sequence of Tspan12 LEL was first cloned into pMal-c2x to link with the coding sequence of the maltose binding protein (MBP). Then the DNA of MBP-tagged Tspan12 LEL (MBP-TSPAN12 LEL) was cloned to multiple cloning site 1 of vector pETDuet-1 after PCR amplification, restriction of enzyme digestion and T4 ligase reaction. DNA of DsbC was cloned into multiple cloning site 2 of vector pETDuet-1 and co-expressed with MBP-TSPAN12 LEL in order to facilitate disulfide bond formation. After transforming the recombinant plasmid into OrigamiB (DE3), MBP-TSPAN12 LEL was expressed by isopropyl-β-d-thiogalactoside induction and purified by amylose resin affinity chromatography and anion exchange chromatography. Results: Sequencing results suggested that recombinant plasmid was successfully constructed. SDS-PAGE showed that the molecular weight of the soluble MBP-TSPAN12 LEL was about 60 kD. Abundant, soluble and highly purified fusion protein was acquired after affinity and anion exchange chromatography. Conclusions: The experimental results prove that co-expression DsbC with MBP-TSPAN12 LEL is a practicable way to produce soluble large extracellular loop of human Tspan12 protein in Escherichia coli. Key words: TSPAN12; maltose binding protein; prokaryotic expression; soluble protein; purification
{"title":"Soluble Expression and Purification of the Large Extracellular Loop of the Human TSPAN12 Protein from Escherichia Coli","authors":"Junlan Chuan, T. Xie, Ganggang Wang, Zheng Rong Yang","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.12.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.12.001","url":null,"abstract":"Objective: \u0000To construct a prokaryotic expression plasmid for the large extracellular loop (LEL) of human TSPAN12 protein and obtain highly purified soluble recombinant proteins. \u0000 \u0000 \u0000Methods: \u0000In this experimental study, the coding sequence of Tspan12 LEL was first cloned into pMal-c2x to link with the coding sequence of the maltose binding protein (MBP). Then the DNA of MBP-tagged Tspan12 LEL (MBP-TSPAN12 LEL) was cloned to multiple cloning site 1 of vector pETDuet-1 after PCR amplification, restriction of enzyme digestion and T4 ligase reaction. DNA of DsbC was cloned into multiple cloning site 2 of vector pETDuet-1 and co-expressed with MBP-TSPAN12 LEL in order to facilitate disulfide bond formation. After transforming the recombinant plasmid into OrigamiB (DE3), MBP-TSPAN12 LEL was expressed by isopropyl-β-d-thiogalactoside induction and purified by amylose resin affinity chromatography and anion exchange chromatography. \u0000 \u0000 \u0000Results: \u0000Sequencing results suggested that recombinant plasmid was successfully constructed. SDS-PAGE showed that the molecular weight of the soluble MBP-TSPAN12 LEL was about 60 kD. Abundant, soluble and highly purified fusion protein was acquired after affinity and anion exchange chromatography. \u0000 \u0000 \u0000Conclusions: \u0000The experimental results prove that co-expression DsbC with MBP-TSPAN12 LEL is a practicable way to produce soluble large extracellular loop of human Tspan12 protein in Escherichia coli. \u0000 \u0000 \u0000Key words: \u0000TSPAN12; maltose binding protein; prokaryotic expression; soluble protein; purification","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"19 1","pages":"881-887"},"PeriodicalIF":0.0,"publicationDate":"2019-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84590481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.007
Ou Hai, Fang Liu
Objective: To observe the clinical effects of intravitreal injection of conbercept combined with Ahemd glaucoma valve implant and panretinal photocoagulation treatment for neovascular glaucoma (NVG). Methods: In this retrospective case series study, 16 NVG patients (17 eyes), at the Number 986 Hospital of the Air Force, were treated from June, 2017 to June, 2018 with intravitreal injection of conbercept combined with Ahemd glaucoma valve implants and panretinal photocoagulation. The disappearance of neovascularization, intraocular pressure (IOP) and best corrected visual acuity (BCVA) were compared between preoperative and postoperative conditions. The data were analyzed by repeated measures ANOVA. The IOP between preoperative and every follow-up was analyzed with independent sample t test. Results: After injection, neovascularization was greatly diminshed by 3 d, and completely vanished by 1 month. The preoperative IOP was 43.3±7.6 mmHg, and postoperative IOP was 14.8±4.8 mmHg, 13.7±3.9 mmHg, 14.5±4.6 mmHg, 13.6±5.4 mmHg and 13.9±4.6 mmHg at 3 d, 7 d, 1 month, 3 months, and 6 months, respectively. There were no significant differences in IOP at different postoperative time points (F=9.58, P=0.006). Compared with preoperative levels, there were significant differences at every follow-up (P<0.05). Compared with preoperative measurements, the BCVA of 5 eyes improved. Conclusions: Intravitreal injection of conbercept combined with Ahemd glaucoma valve implants and panretinal photocoagulation is a safe and effective treatment for neovascular glaucoma. Key words: neovascular glaucoma; conbercept; Ahemd glaucoma valve; panretinal photocoagulation; intravitreal injection
{"title":"Intravitreal Injection of Conbercept Combined with Ahemd Glaucoma Valve Implants and Panretinal Photocoagulation Treatment for Neovascular Glaucoma","authors":"Ou Hai, Fang Liu","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.007","url":null,"abstract":"Objective: \u0000To observe the clinical effects of intravitreal injection of conbercept combined with Ahemd glaucoma valve implant and panretinal photocoagulation treatment for neovascular glaucoma (NVG). \u0000 \u0000 \u0000Methods: \u0000In this retrospective case series study, 16 NVG patients (17 eyes), at the Number 986 Hospital of the Air Force, were treated from June, 2017 to June, 2018 with intravitreal injection of conbercept combined with Ahemd glaucoma valve implants and panretinal photocoagulation. The disappearance of neovascularization, intraocular pressure (IOP) and best corrected visual acuity (BCVA) were compared between preoperative and postoperative conditions. The data were analyzed by repeated measures ANOVA. The IOP between preoperative and every follow-up was analyzed with independent sample t test. \u0000 \u0000 \u0000Results: \u0000After injection, neovascularization was greatly diminshed by 3 d, and completely vanished by 1 month. The preoperative IOP was 43.3±7.6 mmHg, and postoperative IOP was 14.8±4.8 mmHg, 13.7±3.9 mmHg, 14.5±4.6 mmHg, 13.6±5.4 mmHg and 13.9±4.6 mmHg at 3 d, 7 d, 1 month, 3 months, and 6 months, respectively. There were no significant differences in IOP at different postoperative time points (F=9.58, P=0.006). Compared with preoperative levels, there were significant differences at every follow-up (P<0.05). Compared with preoperative measurements, the BCVA of 5 eyes improved. \u0000 \u0000 \u0000Conclusions: \u0000Intravitreal injection of conbercept combined with Ahemd glaucoma valve implants and panretinal photocoagulation is a safe and effective treatment for neovascular glaucoma. \u0000 \u0000 \u0000Key words: \u0000neovascular glaucoma; conbercept; Ahemd glaucoma valve; panretinal photocoagulation; intravitreal injection","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"22 1","pages":"838-841"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75871219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.006
L. Fang, Yin Hu, Y. Ling, Yimin Zhong, Ping Liu, Jingyi Luo, Wei Wei
Objective: To evaluate the visual outcomes in children with primary congenital glaucoma (PCG) whose intraocular pressure (IOP) was controlled after surgery and to analyze the risk factors for vision loss. Methods: This was a retrospective case series that included 45 PCG patients (72 eyes) with an IOP≤21 mmHg following glaucoma surgery from June 2017 to February 2018 at Zhongshan Ophthalmic Center. The following information was collected from medical records: Sex, laterality, age at presentation and at initial glaucoma surgery, age at last visit, preoperative IOP, corneal diameter, axial length, type of initial glaucoma surgery, number of surgeries, antiglaucoma medication before initial surgery and at the last visit, best corrected visual acuity (BCVA), cup-disc ratio and retinal nerve fiber layer thicknessat the last visit, and ocular comorbidity. The BCVAs at the last follow-up were categorized into 3 groups: Good (≥0.4), moderate (0.3-0.1) or poor (<0.1). Onivariate and multivariate analyses were performed to determine the risk factors for visual imparirment (BCVA≤0.3). Results: At the last follow-up, the mean logMAR BCVA was 0.61±0.60. A good BCVA was attained in 54%, moderate in 35% and poor in 11%. The mean spherical equivalent of refraction was -4.07±4.94 D; myopia was the predominant refractive error (50%). The most common ocular comorbidity was corneal opacity (36%). Age at presentation, medication before initial surgery, age at initial glaucoma surgery, number of surgeries and corneal opacity were associated with vision impairment (P<0.1). Multivariate logistic regression analysis showed that multiple surgeries (≥2) (OR=6.252, 95%CI: 1.174-33.285, P=0.032) were associated with BCVA loss. Conclusion: Good BCVA is attainable in about 50% of affected eyes. Multiple surgeries are the main risk factor of vision impairment. Early diagnosis, prompt, effective treatment, and timely management of ocular comorbidities and correction of refractive error safter surgery may reduce the rate of severe visual impairment. Key words: low vision; primary congenital glaucoma; visual acuity; intraocular pressure; surgery
{"title":"Analysis of Visual Outcomes in Children with Primary Congenital Glaucoma","authors":"L. Fang, Yin Hu, Y. Ling, Yimin Zhong, Ping Liu, Jingyi Luo, Wei Wei","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.006","url":null,"abstract":"Objective: \u0000To evaluate the visual outcomes in children with primary congenital glaucoma (PCG) whose intraocular pressure (IOP) was controlled after surgery and to analyze the risk factors for vision loss. \u0000 \u0000 \u0000Methods: \u0000This was a retrospective case series that included 45 PCG patients (72 eyes) with an IOP≤21 mmHg following glaucoma surgery from June 2017 to February 2018 at Zhongshan Ophthalmic Center. The following information was collected from medical records: Sex, laterality, age at presentation and at initial glaucoma surgery, age at last visit, preoperative IOP, corneal diameter, axial length, type of initial glaucoma surgery, number of surgeries, antiglaucoma medication before initial surgery and at the last visit, best corrected visual acuity (BCVA), cup-disc ratio and retinal nerve fiber layer thicknessat the last visit, and ocular comorbidity. The BCVAs at the last follow-up were categorized into 3 groups: Good (≥0.4), moderate (0.3-0.1) or poor (<0.1). Onivariate and multivariate analyses were performed to determine the risk factors for visual imparirment (BCVA≤0.3). \u0000 \u0000 \u0000Results: \u0000At the last follow-up, the mean logMAR BCVA was 0.61±0.60. A good BCVA was attained in 54%, moderate in 35% and poor in 11%. The mean spherical equivalent of refraction was -4.07±4.94 D; myopia was the predominant refractive error (50%). The most common ocular comorbidity was corneal opacity (36%). Age at presentation, medication before initial surgery, age at initial glaucoma surgery, number of surgeries and corneal opacity were associated with vision impairment (P<0.1). Multivariate logistic regression analysis showed that multiple surgeries (≥2) (OR=6.252, 95%CI: 1.174-33.285, P=0.032) were associated with BCVA loss. \u0000 \u0000 \u0000Conclusion: \u0000Good BCVA is attainable in about 50% of affected eyes. Multiple surgeries are the main risk factor of vision impairment. Early diagnosis, prompt, effective treatment, and timely management of ocular comorbidities and correction of refractive error safter surgery may reduce the rate of severe visual impairment. \u0000 \u0000 \u0000Key words: \u0000low vision; primary congenital glaucoma; visual acuity; intraocular pressure; surgery","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"105 1","pages":"831-837"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72924181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.015
Dan Yan, Chen-xi Yan
The tear film covering the entire ocular surface is an important component of the ocular surface micro-environment. The disturbance of the homeostasis of the tear film can result in the dysfunction of other ocular structures, which will exacerbate the development of ocular diseases. This article reviews recent research progress on the effect of tear film on other components of the ocular surface, such as the ocular epithelium, corneal stroma, corneal nerve and ocular microbiota to provide new ideas for the pathogenesis and treatment of ocular surface diseases. Key words: tear film; ocular surface micro-environment; ocular microbiota; sjogren syndrome
{"title":"Research Progress on Tear Film and the Ocular Surface Environment","authors":"Dan Yan, Chen-xi Yan","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.015","url":null,"abstract":"The tear film covering the entire ocular surface is an important component of the ocular surface micro-environment. The disturbance of the homeostasis of the tear film can result in the dysfunction of other ocular structures, which will exacerbate the development of ocular diseases. This article reviews recent research progress on the effect of tear film on other components of the ocular surface, such as the ocular epithelium, corneal stroma, corneal nerve and ocular microbiota to provide new ideas for the pathogenesis and treatment of ocular surface diseases. \u0000 \u0000 \u0000Key words: \u0000tear film; ocular surface micro-environment; ocular microbiota; sjogren syndrome","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"61 1","pages":"877-880"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84658344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.013
Hu Li
{"title":"Misdiagnosis of Intraorbital Foreign Bodies Lead to Penetrating Injury to the Posterior Segment of the Eyeball: A Case Report","authors":"Hu Li","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.013","url":null,"abstract":"","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"303 1","pages":"871-873"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91458090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.004
Haiyan Wang, Qingqing Feng, Jiafan Zhang, W. Ding, Yusu Peng
Objective: To evaluate the clinical effect of diclofenac sodium combined with vitamin C to relieve discomfort after transepithelial photorefractive keratometry (TPRK). Methods: In this randomized control trial, seventy patients undergoing TPRK surgery were recruited from February to June 2018 and divided into observation group and control group, with 35 patients in each group. The observation group was treated with diclofenac sodium eyedrops combined with oral vitamin C, and the control group was treated only with diclofenac sodium eye drops. Symptoms and signs were observed on day 1, day 3 and day 4 after surgery, and eye irritation symptoms were evaluated by a questionnaire, including pain, foreign body feelings, tearing, photophobia, and discomfort in blinking. The results were analyzed with an unpaired t test and repeated measures ANOVA. Results: There were statistically significant differences between the two groups (pain score: F=10.219, P=0.002; foreign body feelings score: F=8.637, P=0.005; tearing: F=5.108, P=0.027. Repeated measures ANOVA). There were statistically significant differences for the above scores between the two groups on day 0 and day 1 after surgery (all P<0.05), but no statistically significant differences on day 2. The differences between the two groups were not statistically significant (photophobia score: F=3.626, P=0.061; discomfort score in blinking: F=2.778, P=0.100). But there were statistically significant differences between the two groups in the total discomfort score (F=7.785, P=0.001). Conclusion: Diclofenac sodium combined with vitamin C can relieve pain after TPRK surgery and reduce discomfort in patients. Key words: transepithelial photorefractive keratectomy; diclofenac sodium; vitamin C; pain; foreign body feeling
{"title":"The Effect of Diclofenac Sodium Combined with Vitamin C for Relieving Discomfort after TPRK","authors":"Haiyan Wang, Qingqing Feng, Jiafan Zhang, W. Ding, Yusu Peng","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.004","url":null,"abstract":"Objective: \u0000To evaluate the clinical effect of diclofenac sodium combined with vitamin C to relieve discomfort after transepithelial photorefractive keratometry (TPRK). \u0000 \u0000 \u0000Methods: \u0000In this randomized control trial, seventy patients undergoing TPRK surgery were recruited from February to June 2018 and divided into observation group and control group, with 35 patients in each group. The observation group was treated with diclofenac sodium eyedrops combined with oral vitamin C, and the control group was treated only with diclofenac sodium eye drops. Symptoms and signs were observed on day 1, day 3 and day 4 after surgery, and eye irritation symptoms were evaluated by a questionnaire, including pain, foreign body feelings, tearing, photophobia, and discomfort in blinking. The results were analyzed with an unpaired t test and repeated measures ANOVA. \u0000 \u0000 \u0000Results: \u0000There were statistically significant differences between the two groups (pain score: F=10.219, P=0.002; foreign body feelings score: F=8.637, P=0.005; tearing: F=5.108, P=0.027. Repeated measures ANOVA). There were statistically significant differences for the above scores between the two groups on day 0 and day 1 after surgery (all P<0.05), but no statistically significant differences on day 2. The differences between the two groups were not statistically significant (photophobia score: F=3.626, P=0.061; discomfort score in blinking: F=2.778, P=0.100). But there were statistically significant differences between the two groups in the total discomfort score (F=7.785, P=0.001). \u0000 \u0000 \u0000Conclusion: \u0000Diclofenac sodium combined with vitamin C can relieve pain after TPRK surgery and reduce discomfort in patients. \u0000 \u0000 \u0000Key words: \u0000transepithelial photorefractive keratectomy; diclofenac sodium; vitamin C; pain; foreign body feeling","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"20 1","pages":"820-825"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90861637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.010
Chen-Xiao Wang, Ji-tuo Gao, Mengmeng Pan, J. Qu
Objective: To design a visual handbook of texts containing reading material from daily life that can be used for self-testing,to provide the effects of visual experience and presbyopic refraction, and to test if the handbook contents can replicate the demands of daily life. Methods: There were two steps in designing this manual of visual effects based on experience. First, a questionnaire was used to obtain frequently used, readable materials from daily life. Two, the contents of the manual were based on the information obtained in the questionnaires. The contents of the handbook were classified into 4 groups, and newspapers books, electrical networks, instructions, and receipts. The materials of each group were arranged in descending order according to the word size of the title, and then printed in color. Results: Two hundred valid questionnaires were collected from adults (18 years and above), including 96 males and 104 females. This visual handbook of texts covers 39 types of reading material from daily life, including newspapers, magazines, telephone directories, short messages, receipts, introductions, etc. The handbook has a total of 50 pages. Song, Regular, Black, and Official scriptare used for the fonts. The word size, wave length and contrast range from 4 to 26 pt, 450 (blue) to 620 (red) nm, and 25% to 90% contrast, respectively. Conclusions: All the contents in this handbook came from reading material encountered in daily life, and background images were added to each category so it was closer to real-life experiences. Hence, the contents in this handbook can reflect the visual demands of daily life, and can be used to assess if the subjects' visual acuity can satisfy these demands. Key words: visual acuity; reading material; visual handbook; daily life
{"title":"The Design of a Visual Handbook of Texts","authors":"Chen-Xiao Wang, Ji-tuo Gao, Mengmeng Pan, J. Qu","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.010","url":null,"abstract":"Objective: \u0000To design a visual handbook of texts containing reading material from daily life that can be used for self-testing,to provide the effects of visual experience and presbyopic refraction, and to test if the handbook contents can replicate the demands of daily life. \u0000 \u0000 \u0000Methods: \u0000There were two steps in designing this manual of visual effects based on experience. First, a questionnaire was used to obtain frequently used, readable materials from daily life. Two, the contents of the manual were based on the information obtained in the questionnaires. The contents of the handbook were classified into 4 groups, and newspapers books, electrical networks, instructions, and receipts. The materials of each group were arranged in descending order according to the word size of the title, and then printed in color. \u0000 \u0000 \u0000Results: \u0000Two hundred valid questionnaires were collected from adults (18 years and above), including 96 males and 104 females. This visual handbook of texts covers 39 types of reading material from daily life, including newspapers, magazines, telephone directories, short messages, receipts, introductions, etc. The handbook has a total of 50 pages. Song, Regular, Black, and Official scriptare used for the fonts. The word size, wave length and contrast range from 4 to 26 pt, 450 (blue) to 620 (red) nm, and 25% to 90% contrast, respectively. \u0000 \u0000 \u0000Conclusions: \u0000All the contents in this handbook came from reading material encountered in daily life, and background images were added to each category so it was closer to real-life experiences. Hence, the contents in this handbook can reflect the visual demands of daily life, and can be used to assess if the subjects' visual acuity can satisfy these demands. \u0000 \u0000 \u0000Key words: \u0000visual acuity; reading material; visual handbook; daily life","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"1 1","pages":"855-859"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89467893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.001
Huankai Zhang, Zhi-wei Li, Jia Wang, Guoying Mu
Objective: To evaluate the effect of ultraviolet A/riboflavin corneal crosslinking (CXL) on keratoconic patients with a corneal thickness less than 400 μm. Methods: In this prospective study, patients with keratoconus were divided into a thin cornea group with a corneal thickness (CT) less than 400 μm and a thick cornea group with a CT more than 400 μm. The patients in the thin and thick cornea groups were treated with a hypotonic or isotonic riboflavin solution before and during CXL. Corneal morphological parameters were evaluated before and after CXL during a 3-year follow-up. The data were analyzed with a repeated measures ANOVA, t test, Wilcoxon rank sum test or Mann-Whitney U test. Results: After surgery, the maximum keratometry (Kmax) of the thin and thick cornea groups underwent a continuous decrease (F= 24.364, P<0.001; F=10.427, P=0.001). In the thin group, the Kmax value was 60.51±6.11 diopters (D) before surgery and significantly decreased to 57.43±6.82 D, 56.13±6.85 D and 54.97±6.66 D at 1, 2 and 3 years after surgery (t=3.670, P=0.002; t=4.637, P<0.001; t=5.816, P<0.001). In the thick group, the Kmax value was 54.56±6.27 D before surgery and significantly decreased to 53.25±6.42 D, 52.32±6.47 D and 51.58±6.70 D at 1, 2 and 3 years after surgery (t=2.266, P=0.040; t=3.302, P=0.005; t=3.769, P=0.002). The Kmax value of the thin cornea group before surgery was higher than that of the thick cornea group (t=2.714, P=0.011). There were no significant differences in Kmax between the two groups at 1 year, 2 years or 3 years. In the thin group, there were significant differences between the preoperative and 3-year postoperative visual acuity (UCVA), best corrected visual acuity (BCVA) and thinnest corneal thickness (TCT) (Z=-2.716, P=0.007; Z=-3.063, P=0.002; t=4.468, P<0.001). In the thick group, there were significant differences between the preoperative and 3-year postoperative UCVA, BCVA and TCT (t=3.572, P=0.003; Z=-2.956, P=0.003; Z=-3.410, P=0.001). In the two groups, there were no significant differences between the preoperative and 3-year postoperative intraocular pressure (IOP) or endothelial cell density (ECD). There were no significant differences in pre- or postoperative UCVA, BCVA, IOP, or ECD between the two groups. There were significiant differences in pre- and postoperative TCT between the two groups (Z=-4.816, P=0.001; Z=-4.024, P<0.001). Conclusion: CXL is an effective and safe way to halt disease progression and improve visual acuity in keratoconic patients with a CT less than 400 μm. Key words: keratoconus; ultraviolet A/riboflavin corneal crosslinking; hypotonic riboflavin solution; isotonic riboflavin solution; corneal thickness
目的:探讨紫外线A/核黄素角膜交联(CXL)治疗角膜厚度小于400 μm角膜屈曲症的疗效。方法:本前瞻性研究将圆锥角膜患者分为角膜厚度小于400 μm的薄角膜组和CT大于400 μm的厚角膜组。薄角膜组和厚角膜组分别在角膜移植前和手术中给予低渗或等渗核黄素溶液。在3年随访期间，评估角膜形态参数在CXL前后。资料分析采用重复测量方差分析、t检验、Wilcoxon秩和检验或Mann-Whitney U检验。结果:术后薄、厚角膜组最大角膜密度(Kmax)持续下降(F= 24.364, P<0.001;F = 10.427, P = 0.001)。瘦组术前Kmax值为60.51±6.11屈光度(D)，术后1、2、3年Kmax值分别为57.43±6.82 D、56.13±6.85 D和54.97±6.66 D，差异有统计学意义(t=3.670, P=0.002;t = 4.637, P < 0.001;t = 5.816, P < 0.001)。厚组术前Kmax值为54.56±6.27 D，术后1、2、3年Kmax值分别为53.25±6.42 D、52.32±6.47 D、51.58±6.70 D，差异有统计学意义(t=2.266, P=0.040;t = 3.302, P = 0.005;t = 3.769, P = 0.002)。薄角膜组术前Kmax值高于厚角膜组(t=2.714, P=0.011)。两组患者在1年、2年和3年的Kmax差异无统计学意义。瘦组术前与术后3年视力(UCVA)、最佳矫正视力(BCVA)、最薄角膜厚度(TCT)差异有统计学意义(Z=-2.716, P=0.007;Z = -3.063, P = 0.002;t = 4.468, P < 0.001)。厚组术前与术后3年UCVA、BCVA、TCT差异有统计学意义(t=3.572, P=0.003;Z = -2.956, P = 0.003;Z = -3.410, P = 0.001)。两组术前和术后3年眼压(IOP)和内皮细胞密度(ECD)无显著差异。两组患者术前和术后UCVA、BCVA、IOP、ECD均无显著差异。两组术前和术后TCT比较差异有统计学意义(Z=-4.816, P=0.001;Z = -4.024, P < 0.001)。结论:对于CT小于400 μm的角膜锥形患者，CXL是一种安全有效的治疗方法。关键词:圆锥角膜;紫外线A/核黄素角膜交联;低渗核黄素溶液;等渗核黄素溶液;角膜厚度
{"title":"The Evaluation of Ultraviolet A/Riboflavin Corneal Crosslinking on Keratoconic Patients with a Corneal Thickness Less than 400 μm: A 3-Year Follow-Up","authors":"Huankai Zhang, Zhi-wei Li, Jia Wang, Guoying Mu","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.001","url":null,"abstract":"Objective: \u0000To evaluate the effect of ultraviolet A/riboflavin corneal crosslinking (CXL) on keratoconic patients with a corneal thickness less than 400 μm. \u0000 \u0000 \u0000Methods: \u0000In this prospective study, patients with keratoconus were divided into a thin cornea group with a corneal thickness (CT) less than 400 μm and a thick cornea group with a CT more than 400 μm. The patients in the thin and thick cornea groups were treated with a hypotonic or isotonic riboflavin solution before and during CXL. Corneal morphological parameters were evaluated before and after CXL during a 3-year follow-up. The data were analyzed with a repeated measures ANOVA, t test, Wilcoxon rank sum test or Mann-Whitney U test. \u0000 \u0000 \u0000Results: \u0000After surgery, the maximum keratometry (Kmax) of the thin and thick cornea groups underwent a continuous decrease (F= 24.364, P<0.001; F=10.427, P=0.001). In the thin group, the Kmax value was 60.51±6.11 diopters (D) before surgery and significantly decreased to 57.43±6.82 D, 56.13±6.85 D and 54.97±6.66 D at 1, 2 and 3 years after surgery (t=3.670, P=0.002; t=4.637, P<0.001; t=5.816, P<0.001). In the thick group, the Kmax value was 54.56±6.27 D before surgery and significantly decreased to 53.25±6.42 D, 52.32±6.47 D and 51.58±6.70 D at 1, 2 and 3 years after surgery (t=2.266, P=0.040; t=3.302, P=0.005; t=3.769, P=0.002). The Kmax value of the thin cornea group before surgery was higher than that of the thick cornea group (t=2.714, P=0.011). There were no significant differences in Kmax between the two groups at 1 year, 2 years or 3 years. In the thin group, there were significant differences between the preoperative and 3-year postoperative visual acuity (UCVA), best corrected visual acuity (BCVA) and thinnest corneal thickness (TCT) (Z=-2.716, P=0.007; Z=-3.063, P=0.002; t=4.468, P<0.001). In the thick group, there were significant differences between the preoperative and 3-year postoperative UCVA, BCVA and TCT (t=3.572, P=0.003; Z=-2.956, P=0.003; Z=-3.410, P=0.001). In the two groups, there were no significant differences between the preoperative and 3-year postoperative intraocular pressure (IOP) or endothelial cell density (ECD). There were no significant differences in pre- or postoperative UCVA, BCVA, IOP, or ECD between the two groups. There were significiant differences in pre- and postoperative TCT between the two groups (Z=-4.816, P=0.001; Z=-4.024, P<0.001). \u0000 \u0000 \u0000Conclusion: \u0000CXL is an effective and safe way to halt disease progression and improve visual acuity in keratoconic patients with a CT less than 400 μm. \u0000 \u0000 \u0000Key words: \u0000keratoconus; ultraviolet A/riboflavin corneal crosslinking; hypotonic riboflavin solution; isotonic riboflavin solution; corneal thickness","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"113 1","pages":"801-806"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75724483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.009
Mingyu Si, Xinxiang Shao
Objective: To investigate the effect and complications of augmentation with a posterior fixation suture in superior rectus transposition (SRT) for abducens nerve palsy. Methods: Two groups of patients with abducens nerve palsy treated with either SRT combined with a posterior fixation suture (A-SRT group, n=10) or SRT (SRT group, n=11) were analyzed retrospectively from March 2014 to January 2019 in Xuzhou No.1 People's Hospital. Changes in esotropia, vertical deviation, cyclotropia, and improvements in abduction and adduction limitation were compared between the two groups. The follow-up time was 10-12 months. The data were analyzed by a Wilcoxon rank sum test and Mann-Whitney test. Results: There were significant differences between the A-SRT and SRT groups in esotropia (Z=-2.940, P<0.001) and abduction limitation (Z=-3.272, P=0.001). The variations in esotropia were 40.0° (36.0°-45.0°) in the A-SRT group and 28.0° (25.0°-30.0°) in the SRT group and the variations in abduction limitation were 2.0 (1.0-2.0) and 1.0 (1.0-1.0), respectively. There was no significant difference between the two groups in adduction limitation (Z=-1.855, P=0.064) or vertical deviation (Z=-1.505, P=0.132). There was a significant difference between the two groups in the variation of subjective in cyclotropia inspected by adouble Maddox rod (Z=-2.228, P=0.026). There was an incyclotorsional shift of 3.0° (0.0°-4.25°) in the A-SRT group and 0.0° (0.0°-2.0°) in the SRT group. But there were no complaints of rotational or vertical discomfort in either group. Conclusion: In augmentation with a posterior fixation suture in SRT for abducens nerve palsy, although there is a possibility of slight in cyclotropia or adduction limitation, abduction limitation was optimally corrected and the correction of esotropic deviation in the primary position was perfect without any obvious subjective complaints of vertical or torsional diplopia. Key words: posterior fixation suture; superior rectus transposition; abducent nerve palsy; cyclotropia; vertical deviation
{"title":"The Effect of Augmentation with a Posterior Fixation Suture in Superior Rectus Transposition for Abducens Nerve Palsy","authors":"Mingyu Si, Xinxiang Shao","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.009","url":null,"abstract":"Objective: \u0000To investigate the effect and complications of augmentation with a posterior fixation suture in superior rectus transposition (SRT) for abducens nerve palsy. \u0000 \u0000 \u0000Methods: \u0000Two groups of patients with abducens nerve palsy treated with either SRT combined with a posterior fixation suture (A-SRT group, n=10) or SRT (SRT group, n=11) were analyzed retrospectively from March 2014 to January 2019 in Xuzhou No.1 People's Hospital. Changes in esotropia, vertical deviation, cyclotropia, and improvements in abduction and adduction limitation were compared between the two groups. The follow-up time was 10-12 months. The data were analyzed by a Wilcoxon rank sum test and Mann-Whitney test. \u0000 \u0000 \u0000Results: \u0000There were significant differences between the A-SRT and SRT groups in esotropia (Z=-2.940, P<0.001) and abduction limitation (Z=-3.272, P=0.001). The variations in esotropia were 40.0° (36.0°-45.0°) in the A-SRT group and 28.0° (25.0°-30.0°) in the SRT group and the variations in abduction limitation were 2.0 (1.0-2.0) and 1.0 (1.0-1.0), respectively. There was no significant difference between the two groups in adduction limitation (Z=-1.855, P=0.064) or vertical deviation (Z=-1.505, P=0.132). There was a significant difference between the two groups in the variation of subjective in cyclotropia inspected by adouble Maddox rod (Z=-2.228, P=0.026). There was an incyclotorsional shift of 3.0° (0.0°-4.25°) in the A-SRT group and 0.0° (0.0°-2.0°) in the SRT group. But there were no complaints of rotational or vertical discomfort in either group. \u0000 \u0000 \u0000Conclusion: \u0000In augmentation with a posterior fixation suture in SRT for abducens nerve palsy, although there is a possibility of slight in cyclotropia or adduction limitation, abduction limitation was optimally corrected and the correction of esotropic deviation in the primary position was perfect without any obvious subjective complaints of vertical or torsional diplopia. \u0000 \u0000 \u0000Key words: \u0000posterior fixation suture; superior rectus transposition; abducent nerve palsy; cyclotropia; vertical deviation","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"11 4 1","pages":"849-854"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78255811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-25DOI: 10.3760/CMA.J.ISSN.1674-845X.2019.11.002
Wen-jie Liu, Jie-wei Liu, Jing-jing Wang, Qi Wang
Objective: To evaluate the characteristics of posterior corneal astigmatism (PA) in cataract patients with high myopia. Methods: In this series of case study, 215 eyes of 215 patients from Shanxi Eye Hospital were recruited from January to June 2018 and divided into a high myopia group (94 patients, 94 eyes) and a control group (121 patients, 121 eyes). PA, total corneal astigmatism (TA) and simulated keratometric astigmatism (KA) were measured by arotating Scheimplfulg camera (Pentacam HR). The error produced by TA using KA was calculated with vector and arithmetical analysis. Data were analyzed by t test and correlation analysis Results: In the high myopia group, the mean magnitude of the pa was -0.33±0.20 D, and exceeded 0.5 D in 30.8%. The steepest meridian was vertically aligned in 79.6% of the cases. In the control group, the mean magnitude of the PA was -0.31±0.18 D. There was no significant difference in PA between the high myopia group and the control group (t=0.589, P=0.557). In the high myopia group, the vector error between KA and TA was (0.12±0.21) D@4°. There was a correlation between the magnitude of PA and KA, PA and Km (KA), and PA and Km (PA) (r=0.340, P=0.001; r=0.285, P=0.006; r=0.333, P=0.001). For KA>0.5 D patients, the difference in magnitude between ta and Ka significantly increased with an increase in the magnitude of pa (r=0.235, P=0.004). Conclusion: There was no significant difference in PA between the high myopia group and the control group. In the high myopia group, neglecting PA may lead to an incorrect estimate of ta. Personalized PA should be considered in patients with Toric intraocular lens implants. Key words: posterior corneal astigmatism; high myopia; cataract
{"title":"Characteristics of Posterior Corneal Astigmatism in Cataract Patients with High Myopia","authors":"Wen-jie Liu, Jie-wei Liu, Jing-jing Wang, Qi Wang","doi":"10.3760/CMA.J.ISSN.1674-845X.2019.11.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1674-845X.2019.11.002","url":null,"abstract":"Objective: \u0000To evaluate the characteristics of posterior corneal astigmatism (PA) in cataract patients with high myopia. \u0000 \u0000 \u0000Methods: \u0000In this series of case study, 215 eyes of 215 patients from Shanxi Eye Hospital were recruited from January to June 2018 and divided into a high myopia group (94 patients, 94 eyes) and a control group (121 patients, 121 eyes). PA, total corneal astigmatism (TA) and simulated keratometric astigmatism (KA) were measured by arotating Scheimplfulg camera (Pentacam HR). The error produced by TA using KA was calculated with vector and arithmetical analysis. Data were analyzed by t test and correlation analysis \u0000 \u0000 \u0000Results: \u0000In the high myopia group, the mean magnitude of the pa was -0.33±0.20 D, and exceeded 0.5 D in 30.8%. The steepest meridian was vertically aligned in 79.6% of the cases. In the control group, the mean magnitude of the PA was -0.31±0.18 D. There was no significant difference in PA between the high myopia group and the control group (t=0.589, P=0.557). In the high myopia group, the vector error between KA and TA was (0.12±0.21) D@4°. There was a correlation between the magnitude of PA and KA, PA and Km (KA), and PA and Km (PA) (r=0.340, P=0.001; r=0.285, P=0.006; r=0.333, P=0.001). For KA>0.5 D patients, the difference in magnitude between ta and Ka significantly increased with an increase in the magnitude of pa (r=0.235, P=0.004). \u0000 \u0000 \u0000Conclusion: \u0000There was no significant difference in PA between the high myopia group and the control group. In the high myopia group, neglecting PA may lead to an incorrect estimate of ta. Personalized PA should be considered in patients with Toric intraocular lens implants. \u0000 \u0000 \u0000Key words: \u0000posterior corneal astigmatism; high myopia; cataract","PeriodicalId":10142,"journal":{"name":"Chinese Journal of Optometry & Ophthalmology","volume":"88 1","pages":"807-812"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82677607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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