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ON THE PRECIPITIN REACTION IN BOVINE CONTAGIOUS PLEURO-PNEUMONIA 牛传染性胸膜肺炎的沉淀反应
Pub Date : 1926-07-20 DOI: 10.1292/JVMS1922.10.153
S. Ono
In the previous report (Journal of the Japanese Society of Veterinary Science, Vol. IV, No. 1) the author called attention to the precipitin reaction regarding the differential diagnosis of bovine contagious pleuro-pneumonia. In the experiment which he has made later on, he used as precipitin the serum obtainee from affected cattle or calves infected by inoculation with the virus, and found that the precipitin serum prepared in this way was not fit for use, because the normal cattle serum may sometimes contain precipitin. For this reason he intended to get a precipitin serum from rabbit and found it very suitable for the object.Preparation of precipitin serum: From a horse-serum-broth-agar slant culture of the virus kept for 10 days in an incubator at 70°C., an emulsion was prepared with 20c.c. of physiological salt solution (precipitogen). One half (10c.c.) of this emulsion was injected into testicle and the other half into thoracic cavity of a rabbit. At an interval of 4 days, the injection was repeated in the same way, and after 15 days the serum was taken from the animal and tested upon precipitin, as shown in the following table, the results being read after one hour at room temperature (10-15°C.).Precipitation experiments have been made in 4 cases of naturally affected cattle, 9 cases of artificially infected calves and 57 cases of controls. The results obtained are shown in the following table:It must be noted here that in the precipitation experiment, all 4 cases of the affected cattle gave positive reaction in a dilution of from 1:640 to 1:1280 and all 9 cases of infected calves inoculated with the virus the same result in a dilution of from 1:40 to 1:1280, while cases of pericarditis (1), pleuro-pneumonia (1), tuberculosis (1), and influenza (1), as well as 53 cases of controls gave negative results.
在之前的报告(日本兽医学会杂志,第四卷,第1期)中,作者呼吁注意关于牛传染性胸膜肺炎鉴别诊断的沉淀反应。在后来的实验中,他用接种了病毒的患病牛或小牛的血清作为沉淀剂,发现用这种方法制备的沉淀剂血清不适合使用,因为正常的牛血清有时可能含有沉淀剂。因此,他打算从兔子身上获得沉淀血清,发现它非常适合于该对象。沉淀血清的制备:从马血清-肉汤-琼脂倾斜培养的病毒在70°C的培养箱中保存10天。,以20cc配制乳液。生理盐溶液(沉淀剂)。将该乳剂的一半(10cc)注射到家兔睾丸中,另一半注射到胸腔中。每隔4天,以同样的方法重复注射,15天后从动物身上取血清,沉淀后检测,如下表所示,室温(10-15℃)下1小时后读取结果。对4例自然感染的牛、9例人工感染的小牛和57例对照进行了降水试验。获得的结果如下表所示:必须注意,在降水实验,影响的所有4例牛给了阳性反应的稀释1:640 1:1280和感染的所有9例小腿接种病毒相同的结果在一个稀释的1:40 1:1280,虽然心包炎(1)的情况下,pleuro-pneumonia(1),结核(1),和流感(1),以及53例控制负面结果。
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引用次数: 0
DEMONSTRATION OF ANTHRAX BACILLUS IN IMPORTED HIDES AND ANIMAL HAIRS 证明进口兽皮及动物毛含有炭疽杆菌
Pub Date : 1926-07-20 DOI: 10.1292/JVMS1922.5.98
H. Migita, 後藤 綾次郎, 島谷 種三郎, 久人 吉川, 澤田 與四郎, 村上 眞志夫
The hides estimated 10, 000, 000-11, 000, 000kin, and the animal hairs amounted to 600, 000-800, 000kin (with the exception of wool). were imported annually through the Osaka port for recent years.Anthrax cases discovered in the examination of these samples are shown in the following table:Since the year 1921 the importation of animal hairs to Japan, chiefly for the manufacture of brushes, became permissible after a course of disinfection at the Custom House at Osaka, Yokohama, and Tsuruga, but there is no ideal method for disinfection of imported hides as yet.
兽皮估计有1000万到1100万公斤,兽毛有60万到80万公斤(羊毛除外)。近年来每年都通过大阪港进口。从1921年开始,在大阪、横滨和鹤贺的海关经过一系列消毒后,主要用于制造刷子的动物毛获准进口到日本,但至今还没有对进口兽皮进行消毒的理想方法。
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引用次数: 0
A CORYNE-BACILLUS AS A CAUSE OF ABSCESS IN THE FEET OF HENS 引起母鸡足部脓肿的一种芽孢杆菌
Pub Date : 1926-03-23 DOI: 10.1292/JVMS1922.5.21
Yutaka Kawamura
From the abscess of the hen's feet a coryne-bacillus was isolated and necrosis was experimentally produced by inocultation of its pure culture into the soft structure of chicken soles.This bacillus may be considered as a cause of the avian disease mentioned above.Before closing, I wish to express my indebtedness to Dr. N. Nakamura for his helpful suggestions and advice.
从鸡足脓肿中分离出一株杆状芽孢杆菌,并将其纯培养物接种于鸡足底软组织中,进行了产生坏死的实验。这种芽孢杆菌可被认为是引起上述禽类疾病的原因。在结束发言之前,我要对中村博士提出的有益建议和意见表示感谢。
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引用次数: 1
AN EXPERIMENTAL STUDY ON THE PATHOLOGY OF THE BLACK-LEG 黑腿病病理的实验研究
Pub Date : 1926-03-23 DOI: 10.1292/JVMS1922.5.1
H. Morita
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引用次数: 2
NEW CASES OF CONTAGIOUS PUSTULOUS DERMATITIS OF SWINE 猪传染性脓疱性皮炎新病例报告
Pub Date : 1926-03-23 DOI: 10.1292/JVMS1922.5.11
H. Futamura
A certain pustulous skin disease has long been recognized as an epizootic disease among swine in this country and there have been often severe outbreak of this disease since 1917. This disease appears epidemically in early summer and late autumn. The swine seems to be most predisposed. The eruption may occur without fever or any trouble of health, and there have been often observed some cases of chronic form, in which affected animals happened to die of cachexia. Our knowledge regarding the causal agent had been far from complete till my attention was directed to the study of it.
我国猪的一种脓疱性皮肤病早就被认为是猪的一种动物传染病,自1917年以来经常发生严重的疫情。该病流行于夏初和深秋。猪似乎是最容易得病的。爆发时可能没有发烧或任何健康问题,经常观察到一些慢性形式的病例,其中受感染的动物碰巧死于恶病质。在我的注意力转向对因果因素的研究之前,我们对因果因素的认识还远远不够全面。
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引用次数: 0
ON THE TOXIC ACTION OF SARCOSPORIDIOTOXIN AND ITS SEROLOGICAL STUDY 肌孢子二毒素的毒性作用及其血清学研究
Pub Date : 1926-03-23 DOI: 10.1292/JVMS1922.5.25
Shinichi Sato
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引用次数: 3
CONTRIBUTION TO THE BIOLOGICAL AND SEROLOGICAL STUDY OF B. ABORTUS EQUI 马abortus b.的生物学和血清学研究
Pub Date : 1926-03-23 DOI: 10.1292/JVMS1922.5.15
S. Fujimura, T. Toyoshima, Takeo Suenaga
B. abortus equi can be separated from the various members of the paratyphoid-enteritidis group as a special bacillus and is perhaps the only organism causing abortion in mares.
马流产芽孢杆菌可以作为一种特殊的芽孢杆菌从副伤寒肠炎群的各种成员中分离出来,它可能是唯一导致母马流产的微生物。
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引用次数: 0
ON THE EFFECT OF CASTRATION UPON THE GROWTH OF VARIOUS ORGANS IN THE ALBINO RAT 去势对白化大鼠各器官生长的影响
Pub Date : 1924-12-22 DOI: 10.1292/JVMS1922.3.347
Torai Shimamura, S. Matsuba
In the previous number of this journal (Vol. III, No. 3), Matsuba, one of the authors, published the first part of this experiment under the article "On the effect of castration upon the thyroid gland, hypophysis, thymus and adrenal in the albino rat." This paper is the second part of the same experiment, dealing with the growth of various organs and the effect of castration upon them excluding endocrine organs.The material used included 300 rats of male sex, varying from newborn to 300 days of age. The 300 rats were all raised up in our laboratory and divided into 500 lots according to litter and age. One to three rats from each lot which consisted of three to eight rats were fed as controls.All the rats excepting the controls were castrated on the 15th, 30th, 40th, 50th, 60th, 75th, 90th, 120th, and 150th day of age and killed with controls at an interval of about 30 days, extending from 60 days to 300 days of age.During the course of experiment all the rats were weighed every other day and the organs to be examined were obtained immediately after the animal were killed and then precisely weighed.The results obtained from the experiment can be summarized as follows.1. The post-natal growth of the castrated rats was in a fair agreement with that of the controls. No remarkable increase was observed in the body weight of the castrated animals; the fatty condition of the animals employed, possibly due to lack of proper feeding and management, might have caused no difference in the body weight of the castrated and the centrols.2. No effect of castration was observed in the body weight of rats, but the tail length of the castrated animals increased by 5% on an average.3. The hearts of the castrated animals develop as good as those of the controls before 180 days of age, but hereafter they showed decrease in their development. A decrease has also been found in the growth of hearts of animals castrated in younger age than those operated in advanced age.4. The weight of kidneys was less by 20-30% in the castrated than in the controls. In the weight of liver no effect of castration was observed. The spleen of the operated was all heavier than that of the control during a certain course of time after castration, but in the operated animals a decrease was found in its weight after 200 days of age.5. The effect of castration upon the length of long bones was quite remarkable; they have been found especially shorter in the animals castrated before 60 days of age. As in the case of the length, the weight of the long bones was also much less in the animals operaied before 60 days of age.6. The better was the deposit of calcium in the bone, the later the castration was performed. As regards the P and Mg contents in the bone no difference has been constated between the castrated and the controls.7. The resistance of long bones against the artificial fracture seemed to decrease due to castration. The femor was weaker in the animals operated after 60 days of age a
在本刊上一期(第三卷,第3期)中,作者之一Matsuba在“关于阉割对白化大鼠甲状腺、垂体、胸腺和肾上腺的影响”一文中发表了该实验的第一部分。本文是同一实验的第二部分,讨论了除内分泌器官外的各种器官的生长和阉割对它们的影响。所使用的材料包括300只雄性大鼠,从新生儿到300日龄不等。300只大鼠均在本实验室饲养,按胎次、年龄分为500批次。每组3 - 8只大鼠饲喂1 - 3只作为对照组。除对照组外,其余大鼠均于15、30、40、50、60、75、90、120、150日龄进行去势,并与对照组每隔约30 d处死,从60日龄延长至300日龄。在实验过程中,每隔一天称一次体重,并在杀死大鼠后立即取取待测器官进行精确称重。从实验中得到的结果可以总结如下。被阉割的大鼠的出生后生长情况与对照组基本一致。阉割动物的体重没有明显增加;可能由于缺乏适当的喂养和管理,被阉割的动物和未阉割的动物的体重没有差别。2 .去势对大鼠体重无影响,但尾长平均增加5%。在180天之前,被阉割的动物的心脏发育与对照组一样好,但此后它们的发育有所下降。年轻时被阉割的动物心脏的生长也比年老时被阉割的动物心脏的生长要慢。去势组肾脏重量比对照组轻20-30%。肝脏重量未见去势的影响。在阉割后的一定时间内,手术组的脾脏均较对照组重,但在200日龄后,手术组的脾脏重量有所下降。阉割对长骨长度的影响相当显著;在60天以前被阉割的动物中,它们的尾巴特别短。与长度的情况一样,在60天以前接受手术的动物的长骨重量也要小得多。骨中钙沉积越好,去势越晚。至于骨中磷和镁的含量,去势组和对照组之间没有差异。由于阉割,长骨对人工骨折的抵抗力似乎有所下降。60日龄以后手术的大鼠股骨较弱,60日龄以前阉割的大鼠胫骨较弱。被阉割的动物通常拉力更大。控制组的牵引力较弱可能是由于它们不安分的行为。
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引用次数: 0
CORRELATIONS BETWEEN HEMORRHAGIC SEPTICEMIA ORGANISMS 出血性败血症微生物之间的相关性
Pub Date : 1924-12-22 DOI: 10.1292/JVMS1922.3.297
D. Niimi
A number of investigations with regard to correlations usually assumed to exist between hemorrhagic septicemia organisms has hitherto been published, but it is at present yet impossible to differenciate them with distinction, as the results obtained by those who have worked with the organisms are not in agreement.Employing B. bubalisepticus, B. suisepticus, B. avisepticus and B. bovisepticus I have made some experiments in connection with these problems and came to the following conclusions:1. Morphology, growth on ordinary culture media, indol production, hemolytic property, bacteriolysis in bile, fermentation test etc. showed no satisfactory evidence as to their identification.2. No distinction could be obtained from their pathogenicity for mice, rats, guinea-pigs, rabbits and pigeons inoculated or fed with the organisms and also from the postmortem appearances of the experimental animals.3. Cross agglutination test employing an immune serum obtained from the rabbit inoculated with dead organisms showed that the correlation between the organisms is highly complicated and no specificity could be distinguished in any of them. Agglutinin absorption test gave likewise no satisfactory results, although when employed an immune serum from the guinea-pig inoculated with living organisms the occurence of group reactions was less remarkable and consequently in a certain organisms specificity could be distinguished. Complement fixation test with those sera showed there are so many group reactions that no specificity could be found in any of the organisms. If, however, the complement fixation titres were taken into consideration the organisms could be specified in more or less noticeable degree, but not satisfactory enough for the identfication of them.4. Active and passive animal protection tests with mice showed in no case so markedly a specificity as to enable us to identify the organisms.Briefly speaking, it may be assumed that some of hemorrha- gic septicemia organisms may have their specificity, but it is quite impossible to differenciate them by virtue of the specificity which is generally so undistinguished.
迄今为止,已经发表了一些关于通常被认为存在于出血性败血症有机体之间的相关性的研究,但目前还不可能区分它们,因为那些与有机体一起工作的人获得的结果并不一致。我利用bubalisepticus, B. suisepticus, B. avisepticus和B. bovisepticus就这些问题做了一些实验,得出以下结论:形态学、在普通培养基上的生长、吲哚的产生、溶血性能、胆汁中的细菌溶解、发酵试验等对其鉴定都没有令人满意的证据。对接种或喂食这些微生物的小鼠、大鼠、豚鼠、兔子和鸽子的致病性,以及实验动物的死后外观,都没有区别。用接种过死菌的兔免疫血清进行交叉凝集试验,结果表明,死菌之间的相关性非常复杂,没有特异性。凝集素吸收试验同样没有令人满意的结果,尽管当使用接种了活生物体的豚鼠的免疫血清时,群体反应的发生不那么显著,因此在某些生物体中可以区分特异性。对这些血清进行的补体固定试验表明,有太多的群体反应,在任何生物体中都找不到特异性。然而,如果考虑到补体固定滴度,这些生物或多或少可以在明显的程度上被指定,但不足以令人满意地识别它们。在小鼠身上进行的主动和被动动物保护试验没有显示出明显的特异性,使我们能够识别出这些生物体。简单地说,可以假定某些出血性败血症菌可能具有其特异性,但由于通常无法区分的特异性,因此完全不可能区分它们。
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引用次数: 0
INFECTIOUS PNEUMONIA IN SHEEP 绵羊传染性肺炎
Pub Date : 1924-12-22 DOI: 10.1292/JVMS1922.3.321
O. Emoto, D. Niimi
In recent years, especially in summer time, infectious pneumonia has been very prevalent among lambs of the Government Sheep Farm at Tomobe (Prefecture Ibaraki), causing great losses every year. The results of our investigation on the disease are summarized as follows:At post-mortem of animals dead from the disease were demonstrated severe anaemia owing to numerous nematodes in the stomach and intestine, pneumonia catarrhalis purulenta lobaris (part of middle lobe, and outer half of anterior and posterior lobes affected) with pleuritis fibroplastica. The histological examination of the affected part of lung revealed hyperaemia, desquamation of alveolar epithelium, exudation of serous fluid, large mononuclear cells and leucocytes in alveoli, leucocytic and lymphocytic infiltration as well as connective tissue production around the bronchioli.From the affected areas of the lung were isolated Bacillus pyogenes, Bacillus ovisepticus, a small Gram-positive bacillus, Micrococcus catarrhalis, a streptococcus, and a staphylccoccus, of which the first three organisms are to be considered most significant for the disease in view of high percentage of their occurrence in pulmonary lesions, their great virulence for the lamb and laboratory animals, and of intimate immunological relations between these organisms and the serum of affected lambs.As the mixed vaccine prepared from these three organisms gave a good result in laboratory animals and a few lambs treated, double treatments with the vaccine were practised this year on the Government Sheep Farm where the administration of vermifuges was enforced at the same time. As the result of the prophylactic measures cases of the disease and losses to the Farm were less than one-tenth those in the previous years.
近年来,特别是在夏季,友部(茨城县)政府绵羊农场的羔羊中传染性肺炎非常普遍,每年都造成很大的损失。我们对该病的调查结果总结如下:死于该病的动物在尸检时表现出严重的贫血,由于胃和肠内大量的线虫,卡塔性肺炎脓性叶(部分中叶,前叶和后叶的外半部受影响)和纤维增塑性胸膜炎。肺病变部位的组织学检查显示充血,肺泡上皮脱屑,浆液渗出,肺泡内有大的单核细胞和白细胞,白细胞和淋巴细胞浸润,细支周围有结缔组织生成。从肺部感染区域分离出化脓性芽孢杆菌、产卵芽孢杆菌、一种小的革兰氏阳性芽孢杆菌、卡他微球菌、一种链球菌和一种葡萄球菌,其中前三种微生物被认为对该疾病最重要,因为它们在肺部病变中的发生率很高,对羔羊和实验动物的毒性很大,而且这些微生物与受感染羔羊的血清之间存在密切的免疫关系。由于这三种生物制成的混合疫苗在实验动物和一些羔羊身上取得了良好的效果,今年在政府绵羊农场实行了疫苗的双重治疗,同时实施了寄生虫的管理。由于采取了预防措施,该农场的疾病病例和损失不到前几年的十分之一。
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引用次数: 1
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Journal of the Japanese Society of Veterinary Science
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