Resistance training (RT) has been used to increase muscle strength and endurance. In CHF patients, the effect of RT has not been thoroughly investigated. This study was conducted to explore the effect of RT to reverse muscular weaknesses and decreased muscular endurance that have been found in many CHF patients. A prospective-randomized controlled study was used as the research design. The effect of RT on muscular strength and endurance was evaluated in 39 CHF patients with II-III New York Heart Association (NYHA) functional class and left ventricle ejection fraction (LVEF), 27 + 7 (mean + standard deviation) in the exercise group and 28 + 6 in the control group. Initial two tests, knee extensor/flexor and elbow extensor/flexor for measuring strength and endurance were performed one week apart using isokinetic machine. After randomization (exercise group=EG and control group=CG), EG performed 3 months supervised RT in hydraulic machine using incremental approach based on individual response. All patients followed the end-point test. The result showed that muscular strength and endurance in EG increased by 20 + 19% (p <.000) and 25 + 26% (p < .001), respectively while in the CG, strength and endurance nearly unchanged. The difference between groups was significant (p< .006 for strength and p < .003 for endurance). There was no adverse cardiac event occurred during the test and training sessions. In conclusion, resistance training was safe for stable CHF patients and was able to increase muscular strength and endurance.
{"title":"Effect of resistance training on strength and endurance of muscle in chronic heart failure","authors":"Herlina I S Wungouw","doi":"10.33476/JKY.V17I1.191","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.191","url":null,"abstract":"Resistance training (RT) has been used to increase muscle strength and endurance. In CHF patients, the effect of RT has not been thoroughly investigated. This study was conducted to explore the effect of RT to reverse muscular weaknesses and decreased muscular endurance that have been found in many CHF patients. A prospective-randomized controlled study was used as the research design. The effect of RT on muscular strength and endurance was evaluated in 39 CHF patients with II-III New York Heart Association (NYHA) functional class and left ventricle ejection fraction (LVEF), 27 + 7 (mean + standard deviation) in the exercise group and 28 + 6 in the control group. Initial two tests, knee extensor/flexor and elbow extensor/flexor for measuring strength and endurance were performed one week apart using isokinetic machine. After randomization (exercise group=EG and control group=CG), EG performed 3 months supervised RT in hydraulic machine using incremental approach based on individual response. All patients followed the end-point test. The result showed that muscular strength and endurance in EG increased by 20 + 19% (p <.000) and 25 + 26% (p < .001), respectively while in the CG, strength and endurance nearly unchanged. The difference between groups was significant (p< .006 for strength and p < .003 for endurance). There was no adverse cardiac event occurred during the test and training sessions. In conclusion, resistance training was safe for stable CHF patients and was able to increase muscular strength and endurance.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123960817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was aimed to examine the effect of ”lanang” onion extract to accelerate the reduction of erythema inflammation sign in contaminated wound. A true experimental with posttest only control group design was used. The variable observed was a photograph of wound taken throughout the experiment which was then processed by program corel photopoint suite graphic 12, so it could provide average value of the decrease of erythema inflammation sign. Samples consisted of 4 groups, each group was composed of five rats. A series of extract concentration were applied, e.g. 128 mg/ml,256 mg/ml, and 512 mg/ml for the first, second and third group respectively. In addition, 10% povidone iodine was used for the forth group. The result showed that by the eighth day, the average decrease of mean red color intensity or the erythema values were 49.26, 45.44, 45.32 and 62. 96 in the first, second, third and forth group respectively. Statistical analysis employing one way ANOVA showed significant difference for all groups [o.ooo (0.05)], however, significant difference [0,0000 < (0,05)] was found between group 1 and 3, group 1 and 4, group 2 and 3, group 2 and 4, group 3 and 4. Employing simple regression correlation, significance correlation of less than 0.05 and R 0.705 was found for the extract in reducing the erythema. It was concluded that “Lanang” onion extract was better in accelerating the reduction of erythema inflamation sign than that with povidone iodine. It was suggested that further studies particularly using microbiology method were still required to prove this finding.
本研究旨在探讨“兰辣”洋葱提取物对污染创面红斑炎症症状的促进作用。采用纯后测对照组设计的真实验。观察到的变量是在整个实验过程中拍摄的伤口照片，然后通过程序corel photopoint suite graphic 12进行处理，因此它可以提供红斑炎症体征减少的平均值。样品分为4组，每组5只大鼠。第一组、第二组、第三组分别采用128mg /ml、256mg /ml、512mg /ml等不同浓度的提取物。第四组采用10%聚维酮碘。结果表明，到第8天，平均红色强度或红斑值的平均下降幅度分别为49.26、45.44、45.32和62。第一、第二、第三、第四组分别为96个。采用单因素方差分析，各组间差异有统计学意义[0]。而组1与组3、组1与组4、组2与组3、组2与组4、组3与组4之间差异有统计学意义[0,000 <(0,05)]。采用简单回归相关分析，发现黄芪提取物减轻红斑的显著相关小于0.05,R为0.705。结果表明，“拉馕”洋葱提取物在加速减轻红斑炎症征象方面优于聚维酮碘。还需要进一步的研究，特别是利用微生物学方法来证明这一发现。
{"title":"Efek Perawatan Luka Terkontaminasi Dengan Ekstrak Bawang Putih Lanang Dalam Mempercepat Penurunan Eritema","authors":"Y. Utami, Anis Murniati, S. Sumarno","doi":"10.33476/JKY.V17I1.193","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.193","url":null,"abstract":"This study was aimed to examine the effect of ”lanang” onion extract to accelerate the reduction of erythema inflammation sign in contaminated wound. A true experimental with posttest only control group design was used. The variable observed was a photograph of wound taken throughout the experiment which was then processed by program corel photopoint suite graphic 12, so it could provide average value of the decrease of erythema inflammation sign. Samples consisted of 4 groups, each group was composed of five rats. A series of extract concentration were applied, e.g. 128 mg/ml,256 mg/ml, and 512 mg/ml for the first, second and third group respectively. In addition, 10% povidone iodine was used for the forth group. The result showed that by the eighth day, the average decrease of mean red color intensity or the erythema values were 49.26, 45.44, 45.32 and 62. 96 in the first, second, third and forth group respectively. Statistical analysis employing one way ANOVA showed significant difference for all groups [o.ooo (0.05)], however, significant difference [0,0000 < (0,05)] was found between group 1 and 3, group 1 and 4, group 2 and 3, group 2 and 4, group 3 and 4. Employing simple regression correlation, significance correlation of less than 0.05 and R 0.705 was found for the extract in reducing the erythema. It was concluded that “Lanang” onion extract was better in accelerating the reduction of erythema inflamation sign than that with povidone iodine. It was suggested that further studies particularly using microbiology method were still required to prove this finding.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130852891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lupus Nephritis (LN) is a serious manifestation of Systemic Lupus Erythematosus (SLE), that is classified into 6 different classes according to WHO criteria. It has been shown that histological pattern of LN varied significantly among the class and the etiology of the difference in the histological phenotypes remains unknown to date. Several studies indicated association between Th1/Th2 cytokines balance with genetic factor. The present study aims to evaluate the interferron-? (IFNy) / interleukin 4 (IL4) ratio among different histological phenotype of LN patients in Indonesia. The study was conducted in 40 female SLE patients at Dr. Saiful Anwar Hospital, Malang, Indonesia. Histologic phenotypes classification was based on WHO criteria (1995). The serum level of IFNy and IL4 was assayed using ELISA and the association between IFNy-IL4 ratio with the histological phenotypes was statistically analyzed using ANOVA. The study revealed that among 31 SLE subjects undergone renal biopsy, class I/II, class III/IV and class V were observed in 13, 12 and 6 subjects respectively. The IFNy-IL4 ratio in class III/IV subjects was significantly higher compared to those in the other classes (p<0.000). However, the IL4 in the class III/IV was significantly lower than the other classes. The findings indicated that the activity of the Th1 immune response tend to be higher in class III/IV LN patients whereas in class V LN patients, the Th2 response prevail. In conclusion, the study indicated that the IFNy-IL4 cytokine ratio might influence the pathogenesis of LN.
{"title":"Analisis rasio IFNy - IL 4 pada berbagai kelompok Nefritis Lupus","authors":"Kusworini Handono","doi":"10.33476/JKY.V17I2.206","DOIUrl":"https://doi.org/10.33476/JKY.V17I2.206","url":null,"abstract":"Lupus Nephritis (LN) is a serious manifestation of Systemic Lupus Erythematosus (SLE), that is classified into 6 different classes according to WHO criteria. It has been shown that histological pattern of LN varied significantly among the class and the etiology of the difference in the histological phenotypes remains unknown to date. Several studies indicated association between Th1/Th2 cytokines balance with genetic factor. The present study aims to evaluate the interferron-? (IFNy) / interleukin 4 (IL4) ratio among different histological phenotype of LN patients in Indonesia. The study was conducted in 40 female SLE patients at Dr. Saiful Anwar Hospital, Malang, Indonesia. Histologic phenotypes classification was based on WHO criteria (1995). The serum level of IFNy and IL4 was assayed using ELISA and the association between IFNy-IL4 ratio with the histological phenotypes was statistically analyzed using ANOVA. The study revealed that among 31 SLE subjects undergone renal biopsy, class I/II, class III/IV and class V were observed in 13, 12 and 6 subjects respectively. The IFNy-IL4 ratio in class III/IV subjects was significantly higher compared to those in the other classes (p<0.000). However, the IL4 in the class III/IV was significantly lower than the other classes. The findings indicated that the activity of the Th1 immune response tend to be higher in class III/IV LN patients whereas in class V LN patients, the Th2 response prevail. In conclusion, the study indicated that the IFNy-IL4 cytokine ratio might influence the pathogenesis of LN.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126581874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Smoking habit is a pleasure for a person and may be dangerous for the others. The main ingredient of cigarette, kretek and cigar is tobacco. Tobacco has been known to contain a lot of chemical substances including nicotin, alkaloids, safrol, ammonia and tar whichare harmful to health. This study was carried out to depict smokers among community in any characteristics of demographic background. The data of smoking habit among peoples of 15 years old or above were collected from the National Health Survey (Survey Kesehatan Nasional) and Household Health Survey (SKRT) 2001 and 1995. More than 12.000 respondents had been interviewed. The result showed that during the last five years the proportion of smokers was increasing in all age groups among male but decreasing among female. Smokers were most prevalence in low educated population and in rural areas. The prevalence was also found to be higher in Sumatera compared to those in Java or eastern part of Indonesia. It is suggested that serious measures should be taken against the campaign of tobacco company including strengtheninglaw enforcement and enhance punishment to the people or company violating the tobacco regulation.
{"title":"Distribusi perokok berdasarkan berbagai latar belakang demografi (Menurut data Susenas 2001 dan 1995)","authors":"Sarjaini Jamal","doi":"10.33476/JKY.V17I1.200","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.200","url":null,"abstract":"Smoking habit is a pleasure for a person and may be dangerous for the others. The main ingredient of cigarette, kretek and cigar is tobacco. Tobacco has been known to contain a lot of chemical substances including nicotin, alkaloids, safrol, ammonia and tar whichare harmful to health. This study was carried out to depict smokers among community in any characteristics of demographic background. The data of smoking habit among peoples of 15 years old or above were collected from the National Health Survey (Survey Kesehatan Nasional) and Household Health Survey (SKRT) 2001 and 1995. More than 12.000 respondents had been interviewed. The result showed that during the last five years the proportion of smokers was increasing in all age groups among male but decreasing among female. Smokers were most prevalence in low educated population and in rural areas. The prevalence was also found to be higher in Sumatera compared to those in Java or eastern part of Indonesia. It is suggested that serious measures should be taken against the campaign of tobacco company including strengtheninglaw enforcement and enhance punishment to the people or company violating the tobacco regulation.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126054570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Endrini, H. Marsiati, .. J.Suherman, .. O.Fauziah, .. R.Asmah
Liver cancer is one among cancers with increasing incidence in the world. Cola fruit (Cola nitida) is a fruit that is rich in properties and has been known since the Dutch colonial era. This fruit contains ingredients such as those contained in tea and chocolate such as methylxanthine and its derivatives. This study aims to determine the content of antioxidants and cytotoxic effects of cola fruit extracts obtained from Indonesia and Malaysia on liver cancer cell lines. Antioxidant content of fruit extracts of cola was assessed using DPPH (2,2-diphenyl-2-picrylhydrazyl hydrate) and cytotoxic effects were studied using MTT (3 - (4,5-Dimethylthiazol-2-Yl)diphenyltetrazolium bromide -2.5) on human liver cancer cell lines (HepG2). The results showed that cola fruit from Malaysia contained high antioxidant with the IC50 value of 37.2 ?g/mL whereas IC50 of value of its Indonesia’s cola fruit was 66.0 ug / mL. The similar results have been shown in the cytotoxic test using HepG-2 liver cancer cell lines. Malaysia’s cola fruit extract has a smaller IC50 value of 6.5 ?g / mL while the fruit extract of Indonesia’s cola showed IC50 value of 39.5 ?g / mL. These values indicates that the fruit extract of cola is a potential anticancer activities especially on liver cancer. Further studies are required to clarify this hypothesis.
肝癌是世界上发病率不断上升的癌症之一。可乐果(Cola nitida)是一种富含属性的水果，自荷兰殖民时代以来就已为人所知。这种水果含有茶和巧克力中含有的成分，如甲基黄嘌呤及其衍生物。本研究旨在测定印尼和马来西亚可乐果提取物的抗氧化剂含量和对肝癌细胞系的细胞毒作用。采用DPPH(2,2-二苯基-2-picrylhydrazyl hydrate)法测定可乐果提取物的抗氧化含量，采用MTT(3 -(4,5-二甲基噻唑-2-酰基)二苯基溴化四唑-2.5)法研究可乐果提取物对人肝癌细胞株(HepG2)的细胞毒作用。结果表明，马来西亚可乐果含有较高的抗氧化剂，IC50值为37.2 μ g/mL，而印度尼西亚可乐果的IC50值为66.0 μ g/mL，用HepG-2肝癌细胞株进行细胞毒试验也得到了相似的结果。马来西亚可乐果提取物的IC50值为6.5 g / mL，而印度尼西亚可乐果提取物的IC50值为39.5 g / mL，这表明可乐果提取物具有潜在的抗癌活性，特别是对肝癌的抗癌作用。需要进一步的研究来澄清这一假设。
{"title":"Aktivitas antioksidan dan efek sitotoksik ekstrak Kola (Cola nitida) pada kulter sel kanker hati (HepG-2)","authors":"S. Endrini, H. Marsiati, .. J.Suherman, .. O.Fauziah, .. R.Asmah","doi":"10.33476/JKY.V17I1.195","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.195","url":null,"abstract":"Liver cancer is one among cancers with increasing incidence in the world. Cola fruit (Cola nitida) is a fruit that is rich in properties and has been known since the Dutch colonial era. This fruit contains ingredients such as those contained in tea and chocolate such as methylxanthine and its derivatives. This study aims to determine the content of antioxidants and cytotoxic effects of cola fruit extracts obtained from Indonesia and Malaysia on liver cancer cell lines. Antioxidant content of fruit extracts of cola was assessed using DPPH (2,2-diphenyl-2-picrylhydrazyl hydrate) and cytotoxic effects were studied using MTT (3 - (4,5-Dimethylthiazol-2-Yl)diphenyltetrazolium bromide -2.5) on human liver cancer cell lines (HepG2). The results showed that cola fruit from Malaysia contained high antioxidant with the IC50 value of 37.2 ?g/mL whereas IC50 of value of its Indonesia’s cola fruit was 66.0 ug / mL. The similar results have been shown in the cytotoxic test using HepG-2 liver cancer cell lines. Malaysia’s cola fruit extract has a smaller IC50 value of 6.5 ?g / mL while the fruit extract of Indonesia’s cola showed IC50 value of 39.5 ?g / mL. These values indicates that the fruit extract of cola is a potential anticancer activities especially on liver cancer. Further studies are required to clarify this hypothesis.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129638662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Health and nutritional status of female workers are factors determining the quality of human resources in the future. A cross sectional study was done in shoes factory on cutting and trimming department in Tangerang district, Banten. This study was conducted to observe the nutritional enemia profile in this group employing questionaire, anthropometric measurement, physical and laboratory examination. The population was female workers. Subjects were chossen using inclusion criteria. A total of 125 persons were admitted in this study. Overall the prevalence of undernutrition was 23 (18.4%). Anemia was found in 78 (62.4%), consisted of 55 (44%) nutritional anemia and 23 (18.4%) unknown caused. Nutritional anemia were separated to iron deficiency anemia 44 (35.2%),B12 deficiency anemia 2 (1.6%), iron + B12 deficiency anemia 4 (3.2%), iron + folic acid deficiency anemia 2 (1.6%), B12 + folic acid deficiency anemia 2 (1.6%), iron + B12 + folic acid deficiency anemia 1 (0.8%). This study concluded that the nutritional anemia was related significantly (p<0.05) to inadequacy protein and iron intake was well as unpair food pattern. In the future, effort should be done to give adequate nutrients intake especially sources of protein, iron, folic acid and B12 vitamin.
{"title":"Gambaran anemia gizi dan kaitannya dengan asupan serta pola makan pada tenaga kerja wanita di Tangerang, Banteng","authors":"M. A. Suyardi, Ance Andriani, Benny L. Priyatna","doi":"10.33476/JKY.V17I1.194","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.194","url":null,"abstract":"Health and nutritional status of female workers are factors determining the quality of human resources in the future. A cross sectional study was done in shoes factory on cutting and trimming department in Tangerang district, Banten. This study was conducted to observe the nutritional enemia profile in this group employing questionaire, anthropometric measurement, physical and laboratory examination. The population was female workers. Subjects were chossen using inclusion criteria. A total of 125 persons were admitted in this study. Overall the prevalence of undernutrition was 23 (18.4%). Anemia was found in 78 (62.4%), consisted of 55 (44%) nutritional anemia and 23 (18.4%) unknown caused. Nutritional anemia were separated to iron deficiency anemia 44 (35.2%),B12 deficiency anemia 2 (1.6%), iron + B12 deficiency anemia 4 (3.2%), iron + folic acid deficiency anemia 2 (1.6%), B12 + folic acid deficiency anemia 2 (1.6%), iron + B12 + folic acid deficiency anemia 1 (0.8%). This study concluded that the nutritional anemia was related significantly (p<0.05) to inadequacy protein and iron intake was well as unpair food pattern. In the future, effort should be done to give adequate nutrients intake especially sources of protein, iron, folic acid and B12 vitamin.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134379474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.
{"title":"Deteksi Mutasi Gen Gyrase A Porphyromonas Gingivalis Resisten terhadap Ciprofloxacin berdasarkan teknik Polymerase Chain Reaction","authors":"I. Rieuwpassa, M. Hatta","doi":"10.33476/JKY.V17I1.192","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.192","url":null,"abstract":"One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132661268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In globalization era, many schools offered integrated school model, among others are full day school, boarding school, etc. which is characterized by longer learning time than that in the public school at the same level. The extra-learning time would eventually prolong the student’s seating time. The chairs are probably comfortable however spinal back pain might be suffered. The cause of spinal back pain (schoolchildren’s neck, shoulder and back) could not be determined accurately. The aim of this study was to investigate the relationship between neck pain and near looking position while they were sitting to read, write and draw. This was a cross sectional study with analytical observation. The subjects of observation were 124 students of Sekolah Dasar Islam Terpadu (SDIT) Lukman Al-Hakim grade VI, with the age range was 10-12 years. The student’s visual activity was examined and flexion angle of the neck were measured while they were sitting to read, write and draw. Questionnaire was used to collect the frequency and the severity of complaints. The statistical methods used were chi-square and discriminant analysis. The result showed that the correlation coefficients between neck pain with near looking position while they were sitting to read, write and draw were 0,671, 0,693 and 0,632 respectively. It was concluded that there was a strong correlation between neck pain and near looking position while they were sitting to read, write and draw.
在全球化时代，许多学校提供一体化的办学模式，其中包括全日制学校，寄宿学校等，其特点是学习时间比同等水平的公立学校长。额外的学习时间最终会延长学生的坐席时间。椅子可能是舒适的，但脊椎背部疼痛可能会遭受。脊柱背部疼痛(学童的颈部、肩部和背部)的原因无法准确确定。这项研究的目的是调查当他们坐着阅读、写作和绘画时颈部疼痛和近看姿势之间的关系。这是一项具有分析观察的横断面研究。观察对象为124名SDIT Lukman Al-Hakim六年级学生，年龄范围为10-12岁。当学生坐着阅读、写作和绘画时，研究人员检查了他们的视觉活动，并测量了他们颈部的屈曲角度。采用问卷调查的方式收集投诉的频率和严重程度。统计方法为卡方分析和判别分析。结果显示，当他们坐着阅读、写作和绘画时，颈部疼痛与近看姿势的相关系数分别为0,671、0,693和0,632。研究得出的结论是，当他们坐着阅读、写作和绘画时，颈部疼痛与近看的姿势有很强的相关性。
{"title":"Hubungan Antara Rasa Nyeri Di Leher Dengan Posisi Melihat Dekat Ketika Duduk Membaca, Menulis Dan Menggambar","authors":"Achmad Sofwan, Soebijanto Soebijanto, Bambang Soempeno","doi":"10.33476/JKY.V17I1.197","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.197","url":null,"abstract":"In globalization era, many schools offered integrated school model, among others are full day school, boarding school, etc. which is characterized by longer learning time than that in the public school at the same level. The extra-learning time would eventually prolong the student’s seating time. The chairs are probably comfortable however spinal back pain might be suffered. The cause of spinal back pain (schoolchildren’s neck, shoulder and back) could not be determined accurately. The aim of this study was to investigate the relationship between neck pain and near looking position while they were sitting to read, write and draw. This was a cross sectional study with analytical observation. The subjects of observation were 124 students of Sekolah Dasar Islam Terpadu (SDIT) Lukman Al-Hakim grade VI, with the age range was 10-12 years. The student’s visual activity was examined and flexion angle of the neck were measured while they were sitting to read, write and draw. Questionnaire was used to collect the frequency and the severity of complaints. The statistical methods used were chi-square and discriminant analysis. The result showed that the correlation coefficients between neck pain with near looking position while they were sitting to read, write and draw were 0,671, 0,693 and 0,632 respectively. It was concluded that there was a strong correlation between neck pain and near looking position while they were sitting to read, write and draw.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129483115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Endang Purwaningsih, Sri Kadarsih Soejono., Djaswadi Dasuki, Edy Meiyanto
Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.
{"title":"Efek Pentagamavunon-0 Terhadap Konsentrasi CAMP Dan Progesteron Pada Kultur Sel Luteal Yang Mengandung Teofilin","authors":"Endang Purwaningsih, Sri Kadarsih Soejono., Djaswadi Dasuki, Edy Meiyanto","doi":"10.33476/JKY.V17I2.202","DOIUrl":"https://doi.org/10.33476/JKY.V17I2.202","url":null,"abstract":"Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"36 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114131847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study was to investigate the effect of curcumin and pentagamavunonn-0 (PGV-0) on the viability of cultured luteal cells (percent of viable cells). The cultured luteal cells were obtained from corpus luteum of induced 12 immature Sprague Dawley rats by a single 10 IU Pregnat Mare’s Serum Gonadotropin. Subject in this study was 4 days old corpus luteum which was cultured in Minimal Essential Medium (MEM) containing 10% Fetal Bovine Serum. This experiment consisted of 12 groups, e.g. one group as control (solvent), three groups as control with LH and or PGF2 stimulation, and eight groups treated with 100 ?M curcumin and PGV-0 in three replicates. Evaluation was conducted after 24 hours of treatment by observation of viable cells with MTT method. The result showed that the viability of cultured luteal cells with curcumin or PGV-0 only was not significantly different compared to control goups. However, curcumin and PGV-0 on cultured luteal cells stimulated by LH and or PGF2 showed significant differences compared to control or LH and PGF2 only.
{"title":"Efek kurkumin dan pentagamavunon-0 terhadap viabilitas kultur sel luteal","authors":"Endang Purwaningsih","doi":"10.33476/JKY.V17I1.196","DOIUrl":"https://doi.org/10.33476/JKY.V17I1.196","url":null,"abstract":"The purpose of this study was to investigate the effect of curcumin and pentagamavunonn-0 (PGV-0) on the viability of cultured luteal cells (percent of viable cells). The cultured luteal cells were obtained from corpus luteum of induced 12 immature Sprague Dawley rats by a single 10 IU Pregnat Mare’s Serum Gonadotropin. Subject in this study was 4 days old corpus luteum which was cultured in Minimal Essential Medium (MEM) containing 10% Fetal Bovine Serum. This experiment consisted of 12 groups, e.g. one group as control (solvent), three groups as control with LH and or PGF2 stimulation, and eight groups treated with 100 ?M curcumin and PGV-0 in three replicates. Evaluation was conducted after 24 hours of treatment by observation of viable cells with MTT method. The result showed that the viability of cultured luteal cells with curcumin or PGV-0 only was not significantly different compared to control goups. However, curcumin and PGV-0 on cultured luteal cells stimulated by LH and or PGF2 showed significant differences compared to control or LH and PGF2 only.","PeriodicalId":101844,"journal":{"name":"YARSI medical Journal","volume":"314 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116101652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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