Pub Date : 2023-05-25DOI: 10.33808/clinexphealthsci.1029390
Yağmur İRMAK ÇELİK, Meltem Demirgöz Bal
Aim: The aim of this study was to investigate the effect of perineal massage with vaseline on the episiotomy and perineal healing in nulliparous women. Method: The double-blind randomized controlled trial was conducted on 90 nulliparous women. In the interventions group, perineal massage was performed with Vaseline and without Vaseline from the active phase of labor. Control group no interventions were performed. Results: Perineal massage with vaseline in the intervention group significant decreased the rate of episiotomies (40%) compared to other group (p< 0.01). In the control group, the mean labor time was 10 hours 5 min, while in the perineal massage group with vaseline, this duration was determined to be 9 hours. (p
{"title":"The Effects of Petroleum Jelly Perineum Massage on Episiotomy and Perineum Healing in the 1st Stage of Labour: A Randomised Controlled Trial","authors":"Yağmur İRMAK ÇELİK, Meltem Demirgöz Bal","doi":"10.33808/clinexphealthsci.1029390","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1029390","url":null,"abstract":"Aim: The aim of this study was to investigate the effect of perineal massage with vaseline on the episiotomy and perineal healing in nulliparous women. \u0000Method: The double-blind randomized controlled trial was conducted on 90 nulliparous women. In the interventions group, perineal massage was performed with Vaseline and without Vaseline from the active phase of labor. Control group no interventions were performed. Results: Perineal massage with vaseline in the intervention group significant decreased the rate of episiotomies (40%) compared to other group (p< 0.01). In the control group, the mean labor time was 10 hours 5 min, while in the perineal massage group with vaseline, this duration was determined to be 9 hours. (p","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46990564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-06DOI: 10.33808/clinexphealthsci.1082079
E. Güler, Ferdiye Taner, Erdal Şanlidağ, P. Tulay, M. C. Ergoren, B. Baddal, C. Özverel, G. Tuncel, Kaya Süer, T. Şanlıdağ
Objective: As an alternative to RT-qPCR assays used in the diagnosis SARS-CoV-2, antigen-detecting rapid diagnostic tests (Ag-RDTs) are available for the qualitative detection of SARS-CoV-2 in nasopharyngeal swab samples. The aim of this study was to assess the accuracy and reliability of Ag-RDTs as a diagnostic method of detecting SARS-CoV-2 positive cases within a given population. Methods: In first phase of this investigation, 357 nasopharyngeal swab samples were screened for SARS-CoV-2 using Ag-RDTs. For the purposes of this study RT-qPCR was then applied to the same 357 nasopharyngeal swab samples in order to compare the reliability of the two detection methods. In the second phase of this investigation, Ag-RDTs were applied to an additional 75 nasopharyngeal swab samples that were already known to be RT-qPCR positive. Results: In the first phase of this investigation, of the 357 samples screened using Ag-RDTs 14 samples were positive for SARS-CoV-2, in contrast, when RT-qPCR analysis was applied to the same 357 samples no SARS-CoV-2 samples were detected. Therefore, the false antigen positivity was determined to be at 3.9%. In the second phase of this investigation 75 RT-qPCR positive samples were re-evaluated with a rapid antigen test. Twenty-four of the 75 RT-qPCR positive sample were undetected. Conclusion: Solely relying on rapid antigen tests to detect SARS-CoV-2 infections in the community could consequently result in infectious individuals remaining in the population. The impact of false negative rapid test results can be reduced by implementing confirmatory RT-qPCR analysis particularly in symptomatic patients.
{"title":"Comparison of the Rapid Antigen Test to RT-qPCR in Diagnosis of SARS-CoV-2: A University Experience in Northern Cyprus","authors":"E. Güler, Ferdiye Taner, Erdal Şanlidağ, P. Tulay, M. C. Ergoren, B. Baddal, C. Özverel, G. Tuncel, Kaya Süer, T. Şanlıdağ","doi":"10.33808/clinexphealthsci.1082079","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1082079","url":null,"abstract":"Objective: As an alternative to RT-qPCR assays used in the diagnosis SARS-CoV-2, antigen-detecting rapid diagnostic tests (Ag-RDTs) are available for the qualitative detection of SARS-CoV-2 in nasopharyngeal swab samples. The aim of this study was to assess the accuracy and reliability of Ag-RDTs as a diagnostic method of detecting SARS-CoV-2 positive cases within a given population. \u0000Methods: In first phase of this investigation, 357 nasopharyngeal swab samples were screened for SARS-CoV-2 using Ag-RDTs. For the purposes of this study RT-qPCR was then applied to the same 357 nasopharyngeal swab samples in order to compare the reliability of the two detection methods. In the second phase of this investigation, Ag-RDTs were applied to an additional 75 nasopharyngeal swab samples that were already known to be RT-qPCR positive. \u0000Results: In the first phase of this investigation, of the 357 samples screened using Ag-RDTs 14 samples were positive for SARS-CoV-2, in contrast, when RT-qPCR analysis was applied to the same 357 samples no SARS-CoV-2 samples were detected. Therefore, the false antigen positivity was determined to be at 3.9%. In the second phase of this investigation 75 RT-qPCR positive samples were re-evaluated with a rapid antigen test. Twenty-four of the 75 RT-qPCR positive sample were undetected. \u0000Conclusion: Solely relying on rapid antigen tests to detect SARS-CoV-2 infections in the community could consequently result in infectious individuals remaining in the population. The impact of false negative rapid test results can be reduced by implementing confirmatory RT-qPCR analysis particularly in symptomatic patients.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42489072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-02DOI: 10.33808/clinexphealthsci.947603
Tuba Ak, Nesrin İlhan
Objective: The aim of this study was to determine the effect of mental health on health-related quality of life in adolescents. Methods: This cross-sectional study was conducted among 1188 students studying in a public high school in Istanbul, Turkey. Data were collected using the General Health Questionnaire (GHQ-12) and the Turkish Generic Health-Related Quality of Life Questionnaire for adolescents (Kiddo-KINDL). Descriptive statistics, correlation analysis and multiple regression analysis were used in the analysis. Results: The mean age of the adolescents was 16.61±1.17; 56.6% were female. This study shows that mental health was a negatively significantly related to all subscales of health-related quality of life (physical wellbeing, emotional wellbeing, self-esteem, family, friends, school) of adolescents (p
{"title":"The Effect of Mental Health on Health-Related Quality of Life in Adolescents","authors":"Tuba Ak, Nesrin İlhan","doi":"10.33808/clinexphealthsci.947603","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.947603","url":null,"abstract":"Objective: The aim of this study was to determine the effect of mental health on health-related quality of life in adolescents. \u0000Methods: This cross-sectional study was conducted among 1188 students studying in a public high school in Istanbul, Turkey. Data were collected using the General Health Questionnaire (GHQ-12) and the Turkish Generic Health-Related Quality of Life Questionnaire for adolescents (Kiddo-KINDL). Descriptive statistics, correlation analysis and multiple regression analysis were used in the analysis. \u0000Results: The mean age of the adolescents was 16.61±1.17; 56.6% were female. This study shows that mental health was a negatively significantly related to all subscales of health-related quality of life (physical wellbeing, emotional wellbeing, self-esteem, family, friends, school) of adolescents (p","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46219659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-29DOI: 10.33808/clinexphealthsci.904203
L. N. Uzun, Birgül Cerit
Objective: This study aimed to examine the effect of digitalization on nursing practices using the lean approach. Method: This is a descriptive observational study. The data were collected using an activity chart to record nurses’ direct and indirect care practices and personal work and the Value Stream Map to compare and analyze work processes and determine both waste and value areas in the clinics. The study included a total of 15 nurses from two different internal medicine units of a hospital, including one digital clinic that uses digital applications for nurse work processes, and one partial digital clinic that has limited digital applications. The data were analyzed using current state value stream mapping, lean seven waste areas, and future state value stream mapping. Results: In the digital clinic, 748.5 minutes were allocated for direct care, 129.1 minutes for indirect care, and 562.1 minutes for personal work. Total value-added time and non-value-added time was calculated as 1137.1 and 302.9 minutes, respectively. In the partial digital clinic, 623.9 minutes were allocated for direct care, 404.4 minutes for indirect care, and 411.1 minutes for personal work. Total value-added time and non-value-added time was calculated as 1006 and 433.4 minutes, respectively. According to the future state value stream map prepared in line with the improvement suggestions to eliminate unnecessary production, process, movement, transportation, waiting, and error waste in the current state of both clinics, it is predicted that 1354.3 minutes of value-added time will be obtained and non-value-added time will decrease to 85.7 minutes, by spending 910.9 minutes less on direct care, 190.2 minutes less on indirect care, and 259.2 minutes less on personal work. Conclusion: The lean approach creates a repeated opportunity to review and improve processes. Analyzing nursing processes using the lean approach before and after digitalization and reviewing sources of both waste and value will contribute to implementing higher quality nursing care practices more effectively and safely and to using time and staff more efficiently.
{"title":"Effect of Digitalization on Nursing Practices using the Lean Approach","authors":"L. N. Uzun, Birgül Cerit","doi":"10.33808/clinexphealthsci.904203","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.904203","url":null,"abstract":"Objective: This study aimed to examine the effect of digitalization on nursing practices using the lean approach. \u0000Method: This is a descriptive observational study. The data were collected using an activity chart to record nurses’ direct and indirect care practices and personal work and the Value Stream Map to compare and analyze work processes and determine both waste and value areas in the clinics. The study included a total of 15 nurses from two different internal medicine units of a hospital, including one digital clinic that uses digital applications for nurse work processes, and one partial digital clinic that has limited digital applications. The data were analyzed using current state value stream mapping, lean seven waste areas, and future state value stream mapping. \u0000Results: In the digital clinic, 748.5 minutes were allocated for direct care, 129.1 minutes for indirect care, and 562.1 minutes for personal work. Total value-added time and non-value-added time was calculated as 1137.1 and 302.9 minutes, respectively. In the partial digital clinic, 623.9 minutes were allocated for direct care, 404.4 minutes for indirect care, and 411.1 minutes for personal work. Total value-added time and non-value-added time was calculated as 1006 and 433.4 minutes, respectively. According to the future state value stream map prepared in line with the improvement suggestions to eliminate unnecessary production, process, movement, transportation, waiting, and error waste in the current state of both clinics, it is predicted that 1354.3 minutes of value-added time will be obtained and non-value-added time will decrease to 85.7 minutes, by spending 910.9 minutes less on direct care, 190.2 minutes less on indirect care, and 259.2 minutes less on personal work. \u0000Conclusion: The lean approach creates a repeated opportunity to review and improve processes. Analyzing nursing processes using the lean approach before and after digitalization and reviewing sources of both waste and value will contribute to implementing higher quality nursing care practices more effectively and safely and to using time and staff more efficiently.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43179663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.33808/clinexphealthsci.983007
Enise Sürücü, Ayşegül Ergün
Objective: Dysmenorrhea is a common health problem that negatively affects daily life and academic skills when not managed well. This study was conducted to examine the Turkish validity and reliability of the Adolescent Dysmenorrhea Self-Care Scale. Methods: This methodological study was conducted in 7 high schools in Bursa, Turkey. The research was carried out with 1041 high school students who met the participation criteria. The Adolescent Dysmenorrhea Self-Care Scale was adapted to Turkish by using translation-back translation method. The validity of the scale was evaluated with the content validity index, confirmatory factor analysis, similarity and discriminant validity coefficients and reliability, item-total correlation, internal consistency coefficient and test-retest analysis. Results: According to the evaluations of the 10 experts whose opinions were taken for the Content Validity, it is determined that each item scored above 3 (3.52-4.00), and the mean score was 3.92 ± 0.14 and the content validity index was .98. Cronbach’s alpha reliability coefficient was found α = .96. Confirmatory factor analysis fit indexes and similarity and discriminant validity coefficients showed that the scale has sufficient compatibility with the theoretical six-factor structure. Item total correlations of subdimensions were r = .20-.68 and Cronbach's alpha coefficients were .77-.65. Test-retest correlations of the Adolescent Dysmenorrhea Self-Care Scale and its subdimensions were found r = .87-.99. Conclusion: Turkish Adolescent Dysmenorrhea Self-Care Scale is a valid and reliable data collection tool consisting of 40 items and six subdimensions.
{"title":"The Turkish Validity and Reliability of The Adolescent Dysmenorrhea Self-Care Scale","authors":"Enise Sürücü, Ayşegül Ergün","doi":"10.33808/clinexphealthsci.983007","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.983007","url":null,"abstract":"Objective: Dysmenorrhea is a common health problem that negatively affects daily life and academic skills when not managed well. This study was conducted to examine the Turkish validity and reliability of the Adolescent Dysmenorrhea Self-Care Scale. \u0000Methods: This methodological study was conducted in 7 high schools in Bursa, Turkey. The research was carried out with 1041 high school students who met the participation criteria. The Adolescent Dysmenorrhea Self-Care Scale was adapted to Turkish by using translation-back translation method. The validity of the scale was evaluated with the content validity index, confirmatory factor analysis, similarity and discriminant validity coefficients and reliability, item-total correlation, internal consistency coefficient and test-retest analysis. \u0000Results: According to the evaluations of the 10 experts whose opinions were taken for the Content Validity, it is determined that each item scored above 3 (3.52-4.00), and the mean score was 3.92 ± 0.14 and the content validity index was .98. Cronbach’s alpha reliability coefficient was found α = .96. Confirmatory factor analysis fit indexes and similarity and discriminant validity coefficients showed that the scale has sufficient compatibility with the theoretical six-factor structure. Item total correlations of subdimensions were r = .20-.68 and Cronbach's alpha coefficients were .77-.65. Test-retest correlations of the Adolescent Dysmenorrhea Self-Care Scale and its subdimensions were found r = .87-.99. \u0000Conclusion: Turkish Adolescent Dysmenorrhea Self-Care Scale is a valid and reliable data collection tool consisting of 40 items and six subdimensions.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47686043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.33808/clinexphealthsci.1169795
Çiğdem Bi̇lge, Ergul Aslan
Objective: This study aims to determine the effect of sexual counseling on the sexual functions and sexual satisfaction of women who underwent surgical treatment due to gynecologic cancer. Methods: The study sample consisted of 60 women (experimental group n = 30, control group n = 30) with ovarian, endometrial, and cervical cancer. The women in the experimental group received sexual counseling according to PLISSIT, while the women in the control group were not given sexual counseling but were monitored through routine follow-up. The Female Sexual Function Index (FSFI) and the Sexual Satisfaction Scale for Women (SSS-W) were used for the first and last evaluations of the groups. Results: The mean age of the women in the experimental group was 51.87 ± 8.89, while it was 50.47 ± 9.43 in the control group. There was no significant difference between the two groups in terms of sexual function and sexual satisfaction levels in the first evaluation (p>.05), whereas there was a significant difference between the two groups in the final evaluation after the sexual counseling provided to the experimental group (p < .001). When the first and final evaluations of the women were considered, it was observed that 33.3% of the women in the experimental group had adequate sexual functioning in the final evaluation. Conclusion: The sexual counseling carried out using the PLISSIT model for women who were treated for gynecologic cancer positively affected their sexual function and sexual satisfaction levels.
{"title":"The Effect of Post-Operative Sexual Counseling Carried out with PLISSIT Model on Sexual Function and Sexual Satisfaction in Gynecologic Cancers","authors":"Çiğdem Bi̇lge, Ergul Aslan","doi":"10.33808/clinexphealthsci.1169795","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1169795","url":null,"abstract":"Objective: This study aims to determine the effect of sexual counseling on the sexual functions and sexual satisfaction of women who underwent surgical treatment due to gynecologic cancer. \u0000Methods: The study sample consisted of 60 women (experimental group n = 30, control group n = 30) with ovarian, endometrial, and cervical cancer. The women in the experimental group received sexual counseling according to PLISSIT, while the women in the control group were not given sexual counseling but were monitored through routine follow-up. The Female Sexual Function Index (FSFI) and the Sexual Satisfaction Scale for Women (SSS-W) were used for the first and last evaluations of the groups. \u0000Results: The mean age of the women in the experimental group was 51.87 ± 8.89, while it was 50.47 ± 9.43 in the control group. There was no significant difference between the two groups in terms of sexual function and sexual satisfaction levels in the first evaluation (p>.05), whereas there was a significant difference between the two groups in the final evaluation after the sexual counseling provided to the experimental group (p < .001). When the first and final evaluations of the women were considered, it was observed that 33.3% of the women in the experimental group had adequate sexual functioning in the final evaluation. \u0000Conclusion: The sexual counseling carried out using the PLISSIT model for women who were treated for gynecologic cancer positively affected their sexual function and sexual satisfaction levels.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41574206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.33808/clinexphealthsci.959620
Ö. Oruç, D. Yıldırım, V. Kocatepe, İnsaf Demi̇rkiran
Objective: This study aims to evaluate the awareness of infectious disease risks and vaccination behaviors of health professionals. Methods: This descriptive and cross-sectional study was conducted who worked at a research and training hospital, met the inclusion criteria and agreed to participate. Personal information form and Communicable Diseases Risk Awareness Protection Scale (CDRAPS) were used for data collection. The study was completed with 208 health professionals actively working at the hospital. Personal information form and communicable diseases risk awareness and protection scale were collected. Results: In this study, 62% of the participants have had influenza before and 85.1% were vaccinated with at least one of the adult vaccines. 10.6%were formerly vaccinated with pneumococcal vaccine and half of them were vaccinated during the COVID-19. 16.8% of the participants, who were not formerly vaccinated with influenza vaccine, stated their intentions to receive vaccine, 90% decided during the pandemic. Mean CDRAPS score was 155.70±15.47. There was statistically significant relationship between the knowledge of the participants on adult vaccines and the mean scores obtained from the CDRAPS (p=.004) and between the decision to receive influenza vaccination and the CDRAPS scores (p=.047). Conclusion: There was a statistically significant relationship between knowledge on adult vaccines, plans to receive influenza vaccination and the scores obtained from the CDRAPS.
{"title":"Awareness of Infectious Disease Risks and Vaccination Behaviors Among Health Professionals","authors":"Ö. Oruç, D. Yıldırım, V. Kocatepe, İnsaf Demi̇rkiran","doi":"10.33808/clinexphealthsci.959620","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.959620","url":null,"abstract":"Objective: This study aims to evaluate the awareness of infectious disease risks and vaccination behaviors of health professionals. \u0000Methods: This descriptive and cross-sectional study was conducted who worked at a research and training hospital, met the inclusion criteria and agreed to participate. Personal information form and Communicable Diseases Risk Awareness Protection Scale (CDRAPS) were used for data collection. The study was completed with 208 health professionals actively working at the hospital. Personal information form and communicable diseases risk awareness and protection scale were collected. \u0000Results: In this study, 62% of the participants have had influenza before and 85.1% were vaccinated with at least one of the adult vaccines. 10.6%were formerly vaccinated with pneumococcal vaccine and half of them were vaccinated during the COVID-19. 16.8% of the participants, who were not formerly vaccinated with influenza vaccine, stated their intentions to receive vaccine, 90% decided during the pandemic. Mean CDRAPS score was 155.70±15.47. There was statistically significant relationship between the knowledge of the participants on adult vaccines and the mean scores obtained from the CDRAPS (p=.004) and between the decision to receive influenza vaccination and the CDRAPS scores (p=.047). \u0000Conclusion: There was a statistically significant relationship between knowledge on adult vaccines, plans to receive influenza vaccination and the scores obtained from the CDRAPS.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43598403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-11DOI: 10.33808/clinexphealthsci.1218956
Emir Alper Türkoğlu, Fatma Gülruy Aydin
Objective: Serum albumins are major plasma proteins in systemic blood circulation and act as transport proteins for endogenous and exogenous compounds such as drugs. In pharmaceutical applications, it is essential to characterize how drugs bind to serum albumin in the evaluation of drug candidates. Surface plasmon resonance (SPR) is fast, real-time, label-free optical based detection technique that offers the monitoring of molecular interactions, analyzing binding reactions and determining the affinity constants with real-time and high sensitivity. Acetazolamide (AZA) is used in the treatment of epilepsy and glaucoma. Methods: To determine the binding kinetics of AZA-Bovine serum albumin (BSA) interaction, (i) SPR gold sensor surface was functionalized, (ii) amine coupling procedure was applied to activate the surface group and BSA was immobilized on functionalized sensor surface, (iii) the concentration series of AZA (10, 25, 50, 75, 100, 150, 200 and 250 µM) was injected to SPR system and (iv) kinetic values were measured using the software of SPR system. Results: 5 mM MUA was coated for surface functionalization. 250 µg/mL BSA as ligand, 30 µL/min flow rate, 1X PBS buffer (pH 7.4) and 10 mM acetate buffer (pH 5.2) as running and coupling buffers, respectively, were performed for SPR binding study. According to result, equilibrium constant (KD) of AZA-BSA was determined as 67.72 µM. Conclusion: In this study, we investigated the AZA-BSA binding interaction using SPR system based on Kretchmann configuration. The study designed with fast, label-free and real-time approach will provide valuable knowledge for pharmaceutical and clinical applications.
目的:血清白蛋白是体循环中主要的血浆蛋白，是内源性和外源性化合物(如药物)的转运蛋白。在制药应用中，在评估候选药物时，表征药物如何与血清白蛋白结合是至关重要的。表面等离子体共振(SPR)是一种快速、实时、无标记的基于光学的检测技术，可以实时、高灵敏度地监测分子相互作用、分析结合反应和确定亲和常数。乙酰唑胺(AZA)用于治疗癫痫和青光眼。方法:为确定AZA-牛血清白蛋白(BSA)相互作用的结合动力学，(i)将SPR金传感器表面功能化，(ii)采用胺偶联程序激活表面基团，将BSA固定在功能化的传感器表面，(iii)将AZA的浓度系列(10、25、50、75、100、150、200和250µM)注射到SPR系统中，(iv)利用SPR系统软件测量动力学值。结果:5 mM MUA包被表面功能化。以250µg/mL BSA为配体，流速为30µL/min, 1X PBS缓冲液(pH 7.4)和10 mM乙酸缓冲液(pH 5.2)分别作为运行缓冲液和偶联缓冲液进行SPR结合研究。结果表明，AZA-BSA的平衡常数(KD)为67.72µM。结论:本研究利用基于Kretchmann构型的SPR体系研究了AZA-BSA的结合相互作用。该研究采用快速、无标签和实时的方法设计，将为制药和临床应用提供有价值的知识。
{"title":"Surface Plasmon Resonance Binding Study on the Interaction of Acetazolamide and Bovine Serum Albumin","authors":"Emir Alper Türkoğlu, Fatma Gülruy Aydin","doi":"10.33808/clinexphealthsci.1218956","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1218956","url":null,"abstract":"Objective: Serum albumins are major plasma proteins in systemic blood circulation and act as transport proteins for endogenous and exogenous compounds such as drugs. In pharmaceutical applications, it is essential to characterize how drugs bind to serum albumin in the evaluation of drug candidates. Surface plasmon resonance (SPR) is fast, real-time, label-free optical based detection technique that offers the monitoring of molecular interactions, analyzing binding reactions and determining the affinity constants with real-time and high sensitivity. Acetazolamide (AZA) is used in the treatment of epilepsy and glaucoma. \u0000Methods: To determine the binding kinetics of AZA-Bovine serum albumin (BSA) interaction, (i) SPR gold sensor surface was functionalized, (ii) amine coupling procedure was applied to activate the surface group and BSA was immobilized on functionalized sensor surface, (iii) the concentration series of AZA (10, 25, 50, 75, 100, 150, 200 and 250 µM) was injected to SPR system and (iv) kinetic values were measured using the software of SPR system. \u0000Results: 5 mM MUA was coated for surface functionalization. 250 µg/mL BSA as ligand, 30 µL/min flow rate, 1X PBS buffer (pH 7.4) and 10 mM acetate buffer (pH 5.2) as running and coupling buffers, respectively, were performed for SPR binding study. According to result, equilibrium constant (KD) of AZA-BSA was determined as 67.72 µM. \u0000Conclusion: In this study, we investigated the AZA-BSA binding interaction using SPR system based on Kretchmann configuration. The study designed with fast, label-free and real-time approach will provide valuable knowledge for pharmaceutical and clinical applications.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41728203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-07DOI: 10.33808/clinexphealthsci.1185564
Ziba Mokhberi̇oskouei̇, Gökhan Biçim, A. Yılmaz, A. Yalçın
Objective: Curcumin (CUR) was modified with hyaluronic acid (HA) to increase its water solubility and bioavailability. Our aim was to increase the uptake of CUR into the cells that express CD44 receptors and to compare the cellular effects in two different human breast carcinoma cells, MCF-7 and MDA-MB-231. Methods: Hyaluronic acid-curcumin complex (HA-CUR) was synthesized and characterized. MCF-7 and MDA-MB-231 cells were grown under appropriate conditions and the effect of CUR and HA-CUR on cell viability was determined. Apoptosis levels of cells after treatment with CUR and HA-CUR were also measured. CD44 receptor levels of both cells were compared and then apoptosis levels were measured in MDA-MB-231 cells after saturation of CD 44 receptors with HA. In both cells expression of caspase-9 and PARP was analyzed to confirm apoptosis. Results: In MCF-7 cells, the percentage apoptosis level of the CUR group was slightly lower than the HA-CUR group. In MDA-MB-231 cells, no statistically significant difference was found in the CUR group compared to the control group, but the apoptosis level of the HA-CUR group was higher than the control group. CD44 receptor levels were higher in MDA-MB-231 cells compared to MCF-7 cells. Blocking the CD44 receptors reversed the apoptotic effect of HA-CUR in MDA-MB-231 cells. Both CUR and HA-CUR had apoptotic effects in MCF-7 and MDA-MB-231 cells. Conclusion: Conjugation of CUR with HA, which is specific for CD44 receptors aids, in its entry to target cells making it a powerful agent for targeted cancer therapy.
{"title":"Hyaluronic Acid-Curcumin Complex Triggers Apoptotic Pathway in Breast Cancer Cells via CD44 Receptors","authors":"Ziba Mokhberi̇oskouei̇, Gökhan Biçim, A. Yılmaz, A. Yalçın","doi":"10.33808/clinexphealthsci.1185564","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1185564","url":null,"abstract":"Objective: Curcumin (CUR) was modified with hyaluronic acid (HA) to increase its water solubility and bioavailability. Our aim was to increase the uptake of CUR into the cells that express CD44 receptors and to compare the cellular effects in two different human breast carcinoma cells, MCF-7 and MDA-MB-231. \u0000Methods: Hyaluronic acid-curcumin complex (HA-CUR) was synthesized and characterized. \u0000MCF-7 and MDA-MB-231 cells were grown under appropriate conditions and the effect of CUR and HA-CUR on cell viability was determined. Apoptosis levels of cells after treatment with CUR and HA-CUR were also measured. CD44 receptor levels of both cells were compared and then apoptosis levels were measured in MDA-MB-231 cells after saturation of CD 44 receptors with HA. In both cells expression of caspase-9 and PARP was analyzed to confirm apoptosis. \u0000Results: In MCF-7 cells, the percentage apoptosis level of the CUR group was slightly lower than the HA-CUR group. In MDA-MB-231 cells, no statistically significant difference was found in the CUR group compared to the control group, but the apoptosis level of the HA-CUR group was higher than the control group. CD44 receptor levels were higher in MDA-MB-231 cells compared to MCF-7 cells. Blocking the CD44 receptors reversed the apoptotic effect of HA-CUR in MDA-MB-231 cells. Both CUR and HA-CUR had apoptotic effects in MCF-7 and MDA-MB-231 cells. \u0000Conclusion: Conjugation of CUR with HA, which is specific for CD44 receptors aids, in its entry to target cells making it a powerful agent for targeted cancer therapy.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49175721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-04DOI: 10.33808/clinexphealthsci.1125345
İçim Gökkaya, Gülin Renda, Tuğba Subaş, U. Özgen
Objective: Plants have been used to treat ailments since the dawn of humanity. The use of medicinal plants for various purposes such as preventing diseases, treating diseases and supporting medical treatment is increasing day by day. On the other hand, medicinal plants are important sources of raw materials for the pharmaceutical industry. It has been demonstrated that Peganum harmala L. and the phytochemicals it contains have a wide variety of pharmacological activities. P. harmala and its active ingredients can be an important resource for the pharmaceutical industry. In this review, the phytochemistry, pharmacological effects, clinical studies, and toxicity of P. harmala are discussed under the current information. Methods: Studies on P. harmala were searched using Pubmed, Scopus, Science Direct databases, and Google Scholar search engine. As a result of the searches, 96 articles were included in the study. Results: The main group of secondary metabolites responsible for the biological activities of P. harmala is alkaloids. The plant and its isolated secondary plant compounds have been shown to have many pharmacological actions, counting antiamnestic, anticancer, antidepressant, antiinflammatory, cardiovascular, gastroprotective, hepatoprotective, nephroprotective, and vasodilator activities. Studies evaluating the plant's clinical effects have been carried out in recent years. However, it has been recorded in the literature that the use of P. harmala causes poisoning with symptoms such as neurosensory symptoms, visual hallucination, bradycardia, hypotension, agitation, tremor, ataxia, and vomiting. Conclusion: Considering the pharmacological effects, the number of studies on the efficacy and safety of P. harmala and its secondary metabolites should be increased.
{"title":"Phytochemical, Pharmacological, and Toxicological Studies on Peganum harmala L.: An Overview of the Last Decade","authors":"İçim Gökkaya, Gülin Renda, Tuğba Subaş, U. Özgen","doi":"10.33808/clinexphealthsci.1125345","DOIUrl":"https://doi.org/10.33808/clinexphealthsci.1125345","url":null,"abstract":"Objective: Plants have been used to treat ailments since the dawn of humanity. The use of medicinal plants for various purposes such as preventing diseases, treating diseases and supporting medical treatment is increasing day by day. On the other hand, medicinal plants are important sources of raw materials for the pharmaceutical industry. It has been demonstrated that Peganum harmala L. and the phytochemicals it contains have a wide variety of pharmacological activities. P. harmala and its active ingredients can be an important resource for the pharmaceutical industry. In this review, the phytochemistry, pharmacological effects, clinical studies, and toxicity of P. harmala are discussed under the current information. \u0000Methods: Studies on P. harmala were searched using Pubmed, Scopus, Science Direct databases, and Google Scholar search engine. As a result of the searches, 96 articles were included in the study. \u0000Results: The main group of secondary metabolites responsible for the biological activities of P. harmala is alkaloids. The plant and its isolated secondary plant compounds have been shown to have many pharmacological actions, counting antiamnestic, anticancer, antidepressant, antiinflammatory, cardiovascular, gastroprotective, hepatoprotective, nephroprotective, and vasodilator activities. Studies evaluating the plant's clinical effects have been carried out in recent years. However, it has been recorded in the literature that the use of P. harmala causes poisoning with symptoms such as neurosensory symptoms, visual hallucination, bradycardia, hypotension, agitation, tremor, ataxia, and vomiting. Conclusion: Considering the pharmacological effects, the number of studies on the efficacy and safety of P. harmala and its secondary metabolites should be increased.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43395364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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