N. Ohmido, A. Shimoura, S. Kato, S. Isobe, S. Tabata
In this study, kudzu (Pueraria lobata Ohwi), a new biomass plant widely grown in Japan, was investigated by chromosome karyotyping. Four strains of Japanese kudzu were examined, and all were found to contain 22 chromosomes. The 5S and 45S rDNA genes, which contain fundamental repeat sequences used in karyotyping, were determined by fluorescence in situ hybridization (FISH). Our results found one 5S rDNA locus and three 45S rDNA loci on the chromosomes of all four strains, which suggest that Japanese kudzu is not greatly differentiated at the cytological level. A further 11 chromosomes were identified, and the rDNA loci were mapped by chromosome image analysis system IV (CHIAS IV), following which kudzu chromosome karyotyping was performed. This is the first report of cytogenetic analysis of P. lobata, a Japanese kudzu.
{"title":"Kudzu (Pueraria lobata Ohwi) karyotyping using FISH and Chromosome Image Analysis System IV","authors":"N. Ohmido, A. Shimoura, S. Kato, S. Isobe, S. Tabata","doi":"10.11352/SCR.16.17","DOIUrl":"https://doi.org/10.11352/SCR.16.17","url":null,"abstract":"In this study, kudzu (Pueraria lobata Ohwi), a new biomass plant widely grown in Japan, was investigated by chromosome karyotyping. Four strains of Japanese kudzu were examined, and all were found to contain 22 chromosomes. The 5S and 45S rDNA genes, which contain fundamental repeat sequences used in karyotyping, were determined by fluorescence in situ hybridization (FISH). Our results found one 5S rDNA locus and three 45S rDNA loci on the chromosomes of all four strains, which suggest that Japanese kudzu is not greatly differentiated at the cytological level. A further 11 chromosomes were identified, and the rDNA loci were mapped by chromosome image analysis system IV (CHIAS IV), following which kudzu chromosome karyotyping was performed. This is the first report of cytogenetic analysis of P. lobata, a Japanese kudzu.","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76690522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Meiotic behavior of genotypes of Lolium multiflorum Lam. of Brazil","authors":"A. S. Freitas, Andréa Mittelmann, V. H. Techio","doi":"10.11352/SCR.16.23","DOIUrl":"https://doi.org/10.11352/SCR.16.23","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76726160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cytologically, Largidae is a very interesting family as it possesses low diploid number and large chromosome size. So far, only ten of its species belonging to two subfamilies Larginae (6) and Physopeltinae (4) have been analysed karyologically. In the present study male specimens of Physopelta gutta, P. quadriguttata and Neophysopelta schlanbuschi having the same chromosomal formula i.e., 2n=17=12A+2m+X1X2Y, were cytologically examined. The behaviour of the sex chromosomes during prophase-I has been found to be specific to each species. In P. gutta, all the three sex chromosomes remain separate throughout the diffuse stage and prophase-I. In P. quadriguttata, X1 and Y remain closely associated while X2 remains separate at the diffuse stage and prophase-I. In N. schlanbuschi, X1, X2 and Y form a single chromatin body at the diffuse stage that remains so throughout prophase-I. In P. gutta and P. quadriguttata, dispersed C-bands are observed in autosomal bivalents excepting one bivalent in P. gutta which shows two heavy terminal C-bands, while in N. schlanbuschi, all the bivalents are found to be C-negative. Sex chromosomes X1, X2 and Y exhibit heterogeneous C-banding pattern whereas m-chromosomes are found to be C-negative in all the three species. Most of the C-heterochromatin in autosomal bivalents is found to be DAPI/CMA3 bright in P. quadriguttata but in P. gutta, base specificity of C-bands is not resolved. Sex chromosomes X1 and Y are found to be DAPI/CMA3 bright in all the three species while X2 exhibits diverse response to fluorochromes. Differences in banding pattern can serve as useful cytological markers.
{"title":"Species-specific sex chromosome behaviour and banding patterns in three Largid species (Heteroptera)","authors":"Vikas Suman, H. Kaur, Devinder Singh, R. Kaur","doi":"10.11352/SCR.15.31","DOIUrl":"https://doi.org/10.11352/SCR.15.31","url":null,"abstract":"Cytologically, Largidae is a very interesting family as it possesses low diploid number and large chromosome size. So far, only ten of its species belonging to two subfamilies Larginae (6) and Physopeltinae (4) have been analysed karyologically. In the present study male specimens of Physopelta gutta, P. quadriguttata and Neophysopelta schlanbuschi having the same chromosomal formula i.e., 2n=17=12A+2m+X1X2Y, were cytologically examined. The behaviour of the sex chromosomes during prophase-I has been found to be specific to each species. In P. gutta, all the three sex chromosomes remain separate throughout the diffuse stage and prophase-I. In P. quadriguttata, X1 and Y remain closely associated while X2 remains separate at the diffuse stage and prophase-I. In N. schlanbuschi, X1, X2 and Y form a single chromatin body at the diffuse stage that remains so throughout prophase-I. In P. gutta and P. quadriguttata, dispersed C-bands are observed in autosomal bivalents excepting one bivalent in P. gutta which shows two heavy terminal C-bands, while in N. schlanbuschi, all the bivalents are found to be C-negative. Sex chromosomes X1, X2 and Y exhibit heterogeneous C-banding pattern whereas m-chromosomes are found to be C-negative in all the three species. Most of the C-heterochromatin in autosomal bivalents is found to be DAPI/CMA3 bright in P. quadriguttata but in P. gutta, base specificity of C-bands is not resolved. Sex chromosomes X1 and Y are found to be DAPI/CMA3 bright in all the three species while X2 exhibits diverse response to fluorochromes. Differences in banding pattern can serve as useful cytological markers.","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82061929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chromosomal distribution of C-band-positive heterochromatin in eight species of Pomacentridae (Perciformes)","authors":"A. Takai","doi":"10.11352/SCR.15.39","DOIUrl":"https://doi.org/10.11352/SCR.15.39","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83007981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Takayoshi Ishii, Naoya Matsumoto, Hiroyuki Tanaka, E. Elsadig, H. Tsujimoto
{"title":"Evolution of subtelomeric and centromeric repetitive sequences in genus Pennisetum (Poaceae)","authors":"Takayoshi Ishii, Naoya Matsumoto, Hiroyuki Tanaka, E. Elsadig, H. Tsujimoto","doi":"10.11352/SCR.15.53","DOIUrl":"https://doi.org/10.11352/SCR.15.53","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91070956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adriana M C Silva, Patrícia Cristina Vizzotto, Quizzi Maria Cordova Becker, José Reinaldo
The genus Astyanax, one of the largest genera in the family Characidae, comprises a large number of similarly shaped fish species. In this study, the karyotypes of three sympatric species of Astyanax were analyzed. Astyanax asuncionensis showed a karyotype of 2n=50 chromosomes (14M+26SM+10ST/A). Astyanax lineatus also had 2n=50 chromosomes, but its karyotype was different (8M+24SM+18ST/A). Astyanax marionae showed a karyotype of 2n=48 chromosomes (8M+24SM+16ST/ A). Ag-NORs appeared in two pairs of chromosomes in Astyanax marionae and in only one pair in the other two species. The variation in the karyotype formula and number of nucleolar organizer regions highlights the importance of these markers in distinguishing among similarly shaped species.
{"title":"Karyotype description of three sympatric species of Astyanax (Characiformes, Characidae)","authors":"Adriana M C Silva, Patrícia Cristina Vizzotto, Quizzi Maria Cordova Becker, José Reinaldo","doi":"10.11352/SCR.15.27","DOIUrl":"https://doi.org/10.11352/SCR.15.27","url":null,"abstract":"The genus Astyanax, one of the largest genera in the family Characidae, comprises a large number of similarly shaped fish species. In this study, the karyotypes of three sympatric species of Astyanax were analyzed. Astyanax asuncionensis showed a karyotype of 2n=50 chromosomes (14M+26SM+10ST/A). Astyanax lineatus also had 2n=50 chromosomes, but its karyotype was different (8M+24SM+18ST/A). Astyanax marionae showed a karyotype of 2n=48 chromosomes (8M+24SM+16ST/ A). Ag-NORs appeared in two pairs of chromosomes in Astyanax marionae and in only one pair in the other two species. The variation in the karyotype formula and number of nucleolar organizer regions highlights the importance of these markers in distinguishing among similarly shaped species.","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83144215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The evolution of the mammalian Y chromosome","authors":"A. Kuroiwa","doi":"10.11352/SCR.15.61","DOIUrl":"https://doi.org/10.11352/SCR.15.61","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78206279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Karyological relationship of Ranunculus sundaicus (Backer) Eichler (Ranunculaceae) in Java to Ranunculus silerifolius Lév. in the Japanese archipelago","authors":"H. Fujishima","doi":"10.11352/SCR.15.3","DOIUrl":"https://doi.org/10.11352/SCR.15.3","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80399159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoshinori Takeuchi, K. Iizuka, Hiroyuki Koishi, Takuzo Yamada, H. Hoshiba
{"title":"Chromosomes of two species of Japanese fishflies in the genus Parachauliodes (Megaloptera : Corydalidae : Chauliodinae)","authors":"Yoshinori Takeuchi, K. Iizuka, Hiroyuki Koishi, Takuzo Yamada, H. Hoshiba","doi":"10.11352/SCR.15.23","DOIUrl":"https://doi.org/10.11352/SCR.15.23","url":null,"abstract":"","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88551277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The karyotype of the endemic Baikal amphipod Polyacanthiska calceolata Bazikalova, 1937 was analysed for the first time. A diploid number (2n=52) and general chromosome morphology (with a median centromere) are typical amongst Gammaridea. A peculiar feature of the karyotype was its pronounced symmetry caused by the presence of metacentric (equal-armed) chromosomes insignificantly varying in size (from 5 to 8 μm). Sex chromosomes have not been distinguished. Supernumerary B chromosomes were regularly found in the metaphase plates of embryos P. calceolata, and may serve as an adaptive mechanism of this species to perform migrations to areas of gas hydrate seeps.
{"title":"Karyotype of the Baikal amphipod Polyacanthisca calceolata Bazikalova, 1937, (Crustacea, Amphipoda)","authors":"A. Natyaganova, T. Sitnikova","doi":"10.11352/SCR.15.43","DOIUrl":"https://doi.org/10.11352/SCR.15.43","url":null,"abstract":"The karyotype of the endemic Baikal amphipod Polyacanthiska calceolata Bazikalova, 1937 was analysed for the first time. A diploid number (2n=52) and general chromosome morphology (with a median centromere) are typical amongst Gammaridea. A peculiar feature of the karyotype was its pronounced symmetry caused by the presence of metacentric (equal-armed) chromosomes insignificantly varying in size (from 5 to 8 μm). Sex chromosomes have not been distinguished. Supernumerary B chromosomes were regularly found in the metaphase plates of embryos P. calceolata, and may serve as an adaptive mechanism of this species to perform migrations to areas of gas hydrate seeps.","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87588073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：