Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.013
Aiqin Liu, Lei Yu, Lige Zhu, De-ting Zhang
Objective To explore the changes and significance of Klotho protein levels in different stages of chronic kidney disease (CKD). Methods From March 2015 to December 2017, 176 patients with CKD admitted to nephrology department of Inner Mongolia People's Hospital were selected as the study object (CKD group), and 80 healthy patients in our hospital were selected as the control group in the same period.The serum Klotho levels of CKD patients and control group at different stages were detected by double antibody sandwich ELISA, and the differences between each group were compared. Results The serum Klotho level of CKD group ((4.84±1.87) μg/L) was significantly lower than that of the control group ((9.11±3.14) μg/L)(t=13.82, P<0.01). One-way anova showed that estimated renal glomerular filtration rate (eGFR), serum albumin (ALB), hemoglobin (Hb), blood calcium (Ca) and serum Klotho were gradually decreased, while phosphorus (P) and creatinine (Cr) in serum were gradually increased, and the difference was statistically significant among the five stages(all P<0.01). Spearman correlation analysis showed that Klotho level was positively correlated with eGFR and Ca, and negatively correlated with CKD stage, Cr and P (r=0.369, 0.160, -0.200, -0.250, -0.230, all P<0.05). The multiple linear regression equation showed that Klotho level was positively and independently correlated with eGFR (t=3.89, P<0.001), and negatively correlated with CKD staging independently (t=-4.12, P<0.001). Conclusion The expression level of serum Klotho protein in patients with CKD is lower than that of healthy people, and it decreases with the increase of CKD stages, which is closely related to the deterioration of renal function.It can be used as a reference index to evaluate the incidence and severity of CKD. Key words: Chronic kidney disease; Klotho protein; Chronic kidney disease stage; Estimated glomerular filtration rate; Renal function
{"title":"Changes and significance of serum Klotho protein levels in different stages of chronic kidney disease","authors":"Aiqin Liu, Lei Yu, Lige Zhu, De-ting Zhang","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.013","url":null,"abstract":"Objective \u0000To explore the changes and significance of Klotho protein levels in different stages of chronic kidney disease (CKD). \u0000 \u0000 \u0000Methods \u0000From March 2015 to December 2017, 176 patients with CKD admitted to nephrology department of Inner Mongolia People's Hospital were selected as the study object (CKD group), and 80 healthy patients in our hospital were selected as the control group in the same period.The serum Klotho levels of CKD patients and control group at different stages were detected by double antibody sandwich ELISA, and the differences between each group were compared. \u0000 \u0000 \u0000Results \u0000The serum Klotho level of CKD group ((4.84±1.87) μg/L) was significantly lower than that of the control group ((9.11±3.14) μg/L)(t=13.82, P<0.01). One-way anova showed that estimated renal glomerular filtration rate (eGFR), serum albumin (ALB), hemoglobin (Hb), blood calcium (Ca) and serum Klotho were gradually decreased, while phosphorus (P) and creatinine (Cr) in serum were gradually increased, and the difference was statistically significant among the five stages(all P<0.01). Spearman correlation analysis showed that Klotho level was positively correlated with eGFR and Ca, and negatively correlated with CKD stage, Cr and P (r=0.369, 0.160, -0.200, -0.250, -0.230, all P<0.05). The multiple linear regression equation showed that Klotho level was positively and independently correlated with eGFR (t=3.89, P<0.001), and negatively correlated with CKD staging independently (t=-4.12, P<0.001). \u0000 \u0000 \u0000Conclusion \u0000The expression level of serum Klotho protein in patients with CKD is lower than that of healthy people, and it decreases with the increase of CKD stages, which is closely related to the deterioration of renal function.It can be used as a reference index to evaluate the incidence and severity of CKD. \u0000 \u0000 \u0000Key words: \u0000Chronic kidney disease; Klotho protein; Chronic kidney disease stage; Estimated glomerular filtration rate; Renal function","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46018369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.014
Liqin Liu, Jing Zhang
Objective To explore the clinical effect of low frequency ultrasound combined with high frequency ultrasound in the diagnosis of polypoid lesions of gallbladder, and to provide reference for clinical diagnosis. Methods From January 2013 to January 2017, 87 patients with gallbladder polypoid lesions in Hankou Hospital were selected as the subjects, all the patients were checked by low frequency ultrasound and high frequency ultrasound, the results were compared with postoperative pathological findings.The diagnostic effect were compared between the low frequency ultrasound and low frequency ultrasound combined with high frequency ultrasound, and the consistency between the two inspection methods and postoperative pathological results was analyzed. Results The postoperative pathological report showed that among the 87 patients, 59 cases (67.82%) had gallbladder polyp, 6 cases (6.90%) had adenomyosis, 10 cases (11.49%)had gallbladder adenoma, 12 (13.79%) had small nodular gallbladder cancer, 65 (86.67%) had benign polypoid lesions, 73 (97.33%) had low frequency ultrasound combined with high frequency ultrasound.The accuracy of combined high-frequency ultrasound in the diagnosis of benign gallbladder polypoid lesions was significantly higher than that of low frequency ultrasound (χ2=5.797, P<0.05); the specificity and positive predictive value of combined low frequency ultrasound and high frequency ultrasound in the diagnosis of benign gallbladder polypoid lesions were significantly higher than those of low frequency ultrasound(90.00% and 41.18%, 97.33% and 86.67%). The difference was statistically significant (χ2=6.217, 5.797, P=0.013, 0.016). Conclusion Compared with low frequency ultrasound, low frequency ultrasound combined with high frequency ultrasound improves the differential diagnosis effect of gallbladder polypoid lesions, which is worthy of clinical attention. Key words: Polypoid lesions of gallbladder; Low frequency ultrasound; High frequency ultrasound
{"title":"Clinical effect of low frequency ultrasound combined with high frequency ultrasound in the diagnosis of gallbladder polypoid lesions","authors":"Liqin Liu, Jing Zhang","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.014","url":null,"abstract":"Objective \u0000To explore the clinical effect of low frequency ultrasound combined with high frequency ultrasound in the diagnosis of polypoid lesions of gallbladder, and to provide reference for clinical diagnosis. \u0000 \u0000 \u0000Methods \u0000From January 2013 to January 2017, 87 patients with gallbladder polypoid lesions in Hankou Hospital were selected as the subjects, all the patients were checked by low frequency ultrasound and high frequency ultrasound, the results were compared with postoperative pathological findings.The diagnostic effect were compared between the low frequency ultrasound and low frequency ultrasound combined with high frequency ultrasound, and the consistency between the two inspection methods and postoperative pathological results was analyzed. \u0000 \u0000 \u0000Results \u0000The postoperative pathological report showed that among the 87 patients, 59 cases (67.82%) had gallbladder polyp, 6 cases (6.90%) had adenomyosis, 10 cases (11.49%)had gallbladder adenoma, 12 (13.79%) had small nodular gallbladder cancer, 65 (86.67%) had benign polypoid lesions, 73 (97.33%) had low frequency ultrasound combined with high frequency ultrasound.The accuracy of combined high-frequency ultrasound in the diagnosis of benign gallbladder polypoid lesions was significantly higher than that of low frequency ultrasound (χ2=5.797, P<0.05); the specificity and positive predictive value of combined low frequency ultrasound and high frequency ultrasound in the diagnosis of benign gallbladder polypoid lesions were significantly higher than those of low frequency ultrasound(90.00% and 41.18%, 97.33% and 86.67%). The difference was statistically significant (χ2=6.217, 5.797, P=0.013, 0.016). \u0000 \u0000 \u0000Conclusion \u0000Compared with low frequency ultrasound, low frequency ultrasound combined with high frequency ultrasound improves the differential diagnosis effect of gallbladder polypoid lesions, which is worthy of clinical attention. \u0000 \u0000 \u0000Key words: \u0000Polypoid lesions of gallbladder; Low frequency ultrasound; High frequency ultrasound","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49566657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.017
Zhiguang Chen
Objective To observe the effect of peripheral arteriovenous exchange combined with intravenous gamma globulin in the treatment of neonatal hemolysis. Methods Seventy children with neonatal hemolysis admitted to the first affiliated Hospital of China Medical University from January 2013 to May 2018 and who met the indications for peripheral arteriovenous exchange were selected as the study subjects.The patients were divided into peripheral arteriovenous exchange group and " peripheral arteriovenous exchange+ gamma globulin" group by random number table method, with 35 cases in each group.Baseline data of the two groups, changes of serum bilirubin before and after treatment, partial blood biochemical indicators, hospitalization time and jaundice regression time were observed. Results The levels of serum bilirubin ((241.5±48.1), (184.6±26.3), (166.3±18.5), (133.5±20.8) μmol/L) in peripheral arteriovenous exchange + gamma globulin group were significantly lower than those in peripheral arteriovenous exchange group ((299.3±32.5), (225.7±38.9), (195.4±21.1), (173.8±35.4) μmol/L) at 12, 24, 48 and 72 hours after treatment, the difference was significant (P<0.05). RBC in children in two groups after treatment was(4.3±0.8)×1012/L, (4.2±1.0)×1012/L vs.before(5.2±1.1)×1012/L, (6.4±1.3)×1012/L, Hb after treatment in both groups was (125.8±11.2) g/L, (124.9±10.5) g/L vs.before (148.9±26.5) g/L, (159.3±14.6) g/L and reticulocyte count after treatment in both groups were (7.6±2.1)%, (7.3±1.8)% vs.(5.2±1.3)%, (3.1±0.5)% were significantly improved, but the peripheral arteriovenous exchange+ gamma globulin group was significantly better than the peripheral arteriovenous transfusion group, the difference was statistically significant (all P<0.05). The hospitalization time (10.3±1.9) d and jaundice regression time (8.6±0.5) d in the peripheral arteriovenous exchange + gamma globulin group were significantly lower than those in the peripheral arteriovenous exchange group ((15.5±2.6) d, (10.0±1.1) d). The difference was statistically significant(t=9.553, 6.855, P<0.05). The children who had re-hemolytic after treatment in the peripheral arteriovenous exchange + gamma globulin group were significantly lower than the peripheral arteriovenous exchange group (5.7%(2/35) vs.25.7%(9/35)), the difference was statistically significant (χ2=5.285, P=0.022). Conclusion Peripheral arteriovenous exchange combined with intravenous gamma globulin is effective in the treatment of neonatal hemolysis.It can significantly reduce serum bilirubin, improve blood biochemical parameters, shorten hospitalization time and jaundice regression time, and is safe and reliable. Key words: Neonatal hemolysis; Peripheral arteriovenous exchange; Gamma globulin; Therapeutic effect
{"title":"Therapeutic effect of peripheral arteriovenous exchange combined with intravenous gamma globulin on neonatal hemolysis","authors":"Zhiguang Chen","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.017","url":null,"abstract":"Objective \u0000To observe the effect of peripheral arteriovenous exchange combined with intravenous gamma globulin in the treatment of neonatal hemolysis. \u0000 \u0000 \u0000Methods \u0000Seventy children with neonatal hemolysis admitted to the first affiliated Hospital of China Medical University from January 2013 to May 2018 and who met the indications for peripheral arteriovenous exchange were selected as the study subjects.The patients were divided into peripheral arteriovenous exchange group and \" peripheral arteriovenous exchange+ gamma globulin\" group by random number table method, with 35 cases in each group.Baseline data of the two groups, changes of serum bilirubin before and after treatment, partial blood biochemical indicators, hospitalization time and jaundice regression time were observed. \u0000 \u0000 \u0000Results \u0000The levels of serum bilirubin ((241.5±48.1), (184.6±26.3), (166.3±18.5), (133.5±20.8) μmol/L) in peripheral arteriovenous exchange + gamma globulin group were significantly lower than those in peripheral arteriovenous exchange group ((299.3±32.5), (225.7±38.9), (195.4±21.1), (173.8±35.4) μmol/L) at 12, 24, 48 and 72 hours after treatment, the difference was significant (P<0.05). RBC in children in two groups after treatment was(4.3±0.8)×1012/L, (4.2±1.0)×1012/L vs.before(5.2±1.1)×1012/L, (6.4±1.3)×1012/L, Hb after treatment in both groups was (125.8±11.2) g/L, (124.9±10.5) g/L vs.before (148.9±26.5) g/L, (159.3±14.6) g/L and reticulocyte count after treatment in both groups were (7.6±2.1)%, (7.3±1.8)% vs.(5.2±1.3)%, (3.1±0.5)% were significantly improved, but the peripheral arteriovenous exchange+ gamma globulin group was significantly better than the peripheral arteriovenous transfusion group, the difference was statistically significant (all P<0.05). The hospitalization time (10.3±1.9) d and jaundice regression time (8.6±0.5) d in the peripheral arteriovenous exchange + gamma globulin group were significantly lower than those in the peripheral arteriovenous exchange group ((15.5±2.6) d, (10.0±1.1) d). The difference was statistically significant(t=9.553, 6.855, P<0.05). The children who had re-hemolytic after treatment in the peripheral arteriovenous exchange + gamma globulin group were significantly lower than the peripheral arteriovenous exchange group (5.7%(2/35) vs.25.7%(9/35)), the difference was statistically significant (χ2=5.285, P=0.022). \u0000 \u0000 \u0000Conclusion \u0000Peripheral arteriovenous exchange combined with intravenous gamma globulin is effective in the treatment of neonatal hemolysis.It can significantly reduce serum bilirubin, improve blood biochemical parameters, shorten hospitalization time and jaundice regression time, and is safe and reliable. \u0000 \u0000 \u0000Key words: \u0000Neonatal hemolysis; Peripheral arteriovenous exchange; Gamma globulin; Therapeutic effect","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45953728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Articular cartilage injury is one of the most common orthopedic diseases with high morbidity and morbidity, especially in the elderly.Articular cartilage injury causes degenerative changes of articular cartilage, such as osteoarthritis, which can lead to disability, pain during joint movement and deformation of bone and joint.The prevalence of osteoarthritis accounts for 10% ~12% of the total population in the world.It is a common disease.The prevalence of osteoarthritis has increased to 49.7% for the elderly aged over 65 years old (Statistics of the World Health Organization (who) in 2010 show that with the development of social aging and obesity and other adverse factors, these figures will continue to rise.It is known that osteoarthritis is related to aging, trauma, genetic susceptibility, obesity and inflammation, but the specific cause of osteoarthritis has not been fully identified, which leads to many obstacles in clinical treatment of osteoarthritis.At present, most of the clinical and research work in this field is focused on the restoration of cartilage trauma.In this review, we summarize and discuss the methods of cartilage defect repair, as well as the hot spots and directions of future research work. Key words: Cartilage injury; Articular cartilage defects; Osteoarthritis; Therapy
{"title":"Treatment and Research Progress of Articular cartilage injury in HT3SS","authors":"Zhongling Hu, Jiayang Wang, Yishuang Cui, Qian Wang, Hui Zhang, Qi-jia Li, Zhiqiang Wang","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.020","url":null,"abstract":"Objective \u0000Articular cartilage injury is one of the most common orthopedic diseases with high morbidity and morbidity, especially in the elderly.Articular cartilage injury causes degenerative changes of articular cartilage, such as osteoarthritis, which can lead to disability, pain during joint movement and deformation of bone and joint.The prevalence of osteoarthritis accounts for 10% ~12% of the total population in the world.It is a common disease.The prevalence of osteoarthritis has increased to 49.7% for the elderly aged over 65 years old (Statistics of the World Health Organization (who) in 2010 show that with the development of social aging and obesity and other adverse factors, these figures will continue to rise.It is known that osteoarthritis is related to aging, trauma, genetic susceptibility, obesity and inflammation, but the specific cause of osteoarthritis has not been fully identified, which leads to many obstacles in clinical treatment of osteoarthritis.At present, most of the clinical and research work in this field is focused on the restoration of cartilage trauma.In this review, we summarize and discuss the methods of cartilage defect repair, as well as the hot spots and directions of future research work. \u0000 \u0000 \u0000Key words: \u0000Cartilage injury; Articular cartilage defects; Osteoarthritis; Therapy","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47753825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.001
R. Wu, Lyu-Han Wang, Lu Gao, Liang Li, L. Zhong, Yi Zhang, C. Gao, Xiaoyong Han
Objective To investigate the relationship between telomere DNA length of peripheral leukocytes and tumor type and prognosis in non-small cell lung cancer (NSCLC). Methods From June 2016 to April 2017, the thoracic surgery department of Baoding First Central Hospital, Hebei Province was included in the study.The patients with lung cancer and TNM stage were confirmed by pathological examination, excluding the patients with unknown general records and lost visits.Finally, 55 patients were included, including 35 patients in adenocarcinoma group and 20 patients in other non-small cell lung cancer group.The telomere DNA length of peripheral blood leukocytes in patients with non-small cell lung cancer was detected by real-time fluorescence quantitative PCR, and the survival of patients was followed up to analyze the relationship between telomere DNA length of peripheral blood leukocytes and tumor pathological type and prognosis. Results The telomere DNA length (T/s) (1.98±0.69) of adenocarcinoma group was larger than that of other non-small cell lung cancer group (1.43±0.67), P=0.007; there was no significant difference in the telomere DNA length of peripheral blood leukocytes in patients with different TNM stages.According to the length of telomere DNA in peripheral blood, the average survival time of short telomere group was 17.149 months(95%CI=14.696~19.602), which was significantly lower than that of long telomere group (30.857 months, 95%CI=28.515~33.199), the difference was statistically significant (P=0.001); Cox proportional risk regression analysis showed that: telomere DNA length (P=0.041), tumor stage (P=0.007) were independent risk factors for prognosis of patients. Conclusion Telomere DNA length of peripheral blood leukocytes in patients with lung adenocarcinoma is longer than that of other non-small cell lung cancer, and TNM stage and telomere DNA length of peripheral blood leukocytes are independent risk factors for prognosis of non-small cell lung cancer. Key words: Non-small cell lung cancer; Telomere DNA length; Lung cancer; Prognosis
{"title":"Relationship between telomere DNA length of peripheral leukocytes and tumor type and prognosis in patients with non-small cell lung cancer","authors":"R. Wu, Lyu-Han Wang, Lu Gao, Liang Li, L. Zhong, Yi Zhang, C. Gao, Xiaoyong Han","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.001","url":null,"abstract":"Objective \u0000To investigate the relationship between telomere DNA length of peripheral leukocytes and tumor type and prognosis in non-small cell lung cancer (NSCLC). \u0000 \u0000 \u0000Methods \u0000From June 2016 to April 2017, the thoracic surgery department of Baoding First Central Hospital, Hebei Province was included in the study.The patients with lung cancer and TNM stage were confirmed by pathological examination, excluding the patients with unknown general records and lost visits.Finally, 55 patients were included, including 35 patients in adenocarcinoma group and 20 patients in other non-small cell lung cancer group.The telomere DNA length of peripheral blood leukocytes in patients with non-small cell lung cancer was detected by real-time fluorescence quantitative PCR, and the survival of patients was followed up to analyze the relationship between telomere DNA length of peripheral blood leukocytes and tumor pathological type and prognosis. \u0000 \u0000 \u0000Results \u0000The telomere DNA length (T/s) (1.98±0.69) of adenocarcinoma group was larger than that of other non-small cell lung cancer group (1.43±0.67), P=0.007; there was no significant difference in the telomere DNA length of peripheral blood leukocytes in patients with different TNM stages.According to the length of telomere DNA in peripheral blood, the average survival time of short telomere group was 17.149 months(95%CI=14.696~19.602), which was significantly lower than that of long telomere group (30.857 months, 95%CI=28.515~33.199), the difference was statistically significant (P=0.001); Cox proportional risk regression analysis showed that: telomere DNA length (P=0.041), tumor stage (P=0.007) were independent risk factors for prognosis of patients. \u0000 \u0000 \u0000Conclusion \u0000Telomere DNA length of peripheral blood leukocytes in patients with lung adenocarcinoma is longer than that of other non-small cell lung cancer, and TNM stage and telomere DNA length of peripheral blood leukocytes are independent risk factors for prognosis of non-small cell lung cancer. \u0000 \u0000 \u0000Key words: \u0000Non-small cell lung cancer; Telomere DNA length; Lung cancer; Prognosis","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43086218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.012
Hui Zhang, Ying-Jun Zhao, Huili Jiang, L. Wang, Chenghua Liu, Mei Han
Objective To explore the four tumor markers of alpha-fetoprotein (AFP), α-L-fucosidase(AFU), carbohydrate antigen 199 (CA199) and carcinoembryonic antigen (CEA) and their combined use for primary hepatocellular carcinoma (HCC) diagnosis and treatment value. Methods From February 2016 to August 2018, 92 patients with primary hepatocellular carcinoma (HCC group), 79 patients with benign liver disease (chronic hepatitis and cirrhosis group) and 99 healthy adults (control group) were selected as subjects.The serum levels of four tumor markers in different populations were compared. Results The serum levels of four tumor markers (AFP(192.4±89.3) μg/L、AFU(78.6±25.8) U/L、CA199(107.2±59.5) U/mL、CEA(37.9±14.9) μg/L) were significantly higher than those of benign liver disease group(AFP(17.4±6.3) μg/L、AFU(35.4±17.2) U/L、CA199(29.3±15.2) U/mL、CEA(4.9±1.7) μg/L) and normal people(AFP(4.8±1.1) μg/L、AFU(12.2±3.6) U/L、CA199(6.4±2.3) U/mL、CEA(1.8±0.4) μg/L). There differences had significant (all P<0.05). The abnormal rate of single factor in hepatocellular carcinoma group (AFP 84.8%, AFU 52.2%, CA199 41.3%, CEA35.9%) was significantly higher than that in benign liver disease group (AFP 15.2%, AFU 19.0%, CA19916.5%, CEA13.9%) and normal group (AFP 4.0%, AFU 5.0%, CA199 3.0%, CEA 6.0% ug/L), the difference was statistically significant (all P<0.05). The highest sensitivity was AFP (84.8%) and the highest specificity was AFP and CA199 (91.0%). The sensitivity of combined detection was 94.6% higher than that of single index (AFP 84.8%, AFU52.2%, CA199 41.3%, CEA35.9%). Conclusion The combined detection of AFP, AFU, CA199 and CEA can increase the sensitivity of diagnosis of hepatocellular carcinoma and reduce the rate of missed diagnosis, which will be beneficial to the early diagnosis and treatment of hepatocellular carcinoma. Key words: Hepatocellular carcinoma; Alpha-fetoprotein; Alpha-L-fucosidase; Carbohydrate antigen 199; Carcinoembryonic antigen
{"title":"Diagnostic value of multiple serum tumor markers for hepatocellular carcinoma","authors":"Hui Zhang, Ying-Jun Zhao, Huili Jiang, L. Wang, Chenghua Liu, Mei Han","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.012","url":null,"abstract":"Objective \u0000To explore the four tumor markers of alpha-fetoprotein (AFP), α-L-fucosidase(AFU), carbohydrate antigen 199 (CA199) and carcinoembryonic antigen (CEA) and their combined use for primary hepatocellular carcinoma (HCC) diagnosis and treatment value. \u0000 \u0000 \u0000Methods \u0000From February 2016 to August 2018, 92 patients with primary hepatocellular carcinoma (HCC group), 79 patients with benign liver disease (chronic hepatitis and cirrhosis group) and 99 healthy adults (control group) were selected as subjects.The serum levels of four tumor markers in different populations were compared. \u0000 \u0000 \u0000Results \u0000The serum levels of four tumor markers (AFP(192.4±89.3) μg/L、AFU(78.6±25.8) U/L、CA199(107.2±59.5) U/mL、CEA(37.9±14.9) μg/L) were significantly higher than those of benign liver disease group(AFP(17.4±6.3) μg/L、AFU(35.4±17.2) U/L、CA199(29.3±15.2) U/mL、CEA(4.9±1.7) μg/L) and normal people(AFP(4.8±1.1) μg/L、AFU(12.2±3.6) U/L、CA199(6.4±2.3) U/mL、CEA(1.8±0.4) μg/L). There differences had significant (all P<0.05). The abnormal rate of single factor in hepatocellular carcinoma group (AFP 84.8%, AFU 52.2%, CA199 41.3%, CEA35.9%) was significantly higher than that in benign liver disease group (AFP 15.2%, AFU 19.0%, CA19916.5%, CEA13.9%) and normal group (AFP 4.0%, AFU 5.0%, CA199 3.0%, CEA 6.0% ug/L), the difference was statistically significant (all P<0.05). The highest sensitivity was AFP (84.8%) and the highest specificity was AFP and CA199 (91.0%). The sensitivity of combined detection was 94.6% higher than that of single index (AFP 84.8%, AFU52.2%, CA199 41.3%, CEA35.9%). \u0000 \u0000 \u0000Conclusion \u0000The combined detection of AFP, AFU, CA199 and CEA can increase the sensitivity of diagnosis of hepatocellular carcinoma and reduce the rate of missed diagnosis, which will be beneficial to the early diagnosis and treatment of hepatocellular carcinoma. \u0000 \u0000 \u0000Key words: \u0000Hepatocellular carcinoma; Alpha-fetoprotein; Alpha-L-fucosidase; Carbohydrate antigen 199; Carcinoembryonic antigen","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44164034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.010
Q. Shi, Ling-Guo Xu, Rong Yang, Yaping Meng, Xiaofeng Xu, L. Qiu
Objective To analyze the positive expression levels of p16 (p16ink4a), cell cycle factor geminin and Ki-67 in low-grade squamous intraepithelial lesions (LSIL), and to further explore the ability of these indicators to evaluate the progression of LSIL patients. Methods From January 2015 to June 2018, 276 cervical specimens from Jiading District Central Hospital of Shanghai were retrospectively studied, and 148 LSIL patients were selected.According to the results of the second examination, LSIL patients were divided into three groups: (1) no lesion (natural regression) group 90 cases; (2) LSIL persistent group 38 cases; (3) high-grade Squamous Intraepithelial Lesion (HSIL) group 20 cases.Immunohistochemistry was performed on the first biopsy tissues and the relative positive ratios of p16, geminin and Ki-67 were calculated.Spearman correlation analysis identified the correlation between the above indicators and the progress of the disease; ROC curve was used to calculate the best diagnostic value of each indicator, and multivariate logistic regression analysis was included to explore the ability of the above indicators to assess the risk of patients progressing to HSIL. Results In the HSIL group, p16 (51.26±17.15)%, geminin relative positive ratio (45.92±15.70) % was higher than those in the LSIL group((43.71±11.84)%, (21.68±14.47)%) and regression group((17.92±9.60)%, (0.16±0.03)%). The difference were statistically significant (F=2.922, 2.751, all P<0.05). Spearman correlation analysis showed that the relative positive ratio of p16 (r=0.27, P=0.014) and geminin (r=0.44, P<0.001) presented a notable positive correlation with the progression of the disease.Under the ROC curve, the best diagnostic values of p16, geminin and Ki-67 were 38.9%, 32.5% and 18.6%, respectively.Multivariate logistic regression analysis showed that the relative positive ratio of p16 was higher than 38.9% (OR=4.366, P=0.006), and geminin was higher than 32.5% (OR=5.392, P=0.011) had a higher risk of progression to HSIL. Conclusion p16 and geminin may be effective biomarkers for identifying patients with advanced LSIL. Key words: p16; Cell cycle factor geminin; Ki-67; Low-grade squamous intraepithelial lesions; High-grade squamous intraepithelial lesions
{"title":"Clinical value of p16 and geminin in the progress of patients with low-grade squamous intraepithelial lesions","authors":"Q. Shi, Ling-Guo Xu, Rong Yang, Yaping Meng, Xiaofeng Xu, L. Qiu","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.010","url":null,"abstract":"Objective \u0000To analyze the positive expression levels of p16 (p16ink4a), cell cycle factor geminin and Ki-67 in low-grade squamous intraepithelial lesions (LSIL), and to further explore the ability of these indicators to evaluate the progression of LSIL patients. \u0000 \u0000 \u0000Methods \u0000From January 2015 to June 2018, 276 cervical specimens from Jiading District Central Hospital of Shanghai were retrospectively studied, and 148 LSIL patients were selected.According to the results of the second examination, LSIL patients were divided into three groups: (1) no lesion (natural regression) group 90 cases; (2) LSIL persistent group 38 cases; (3) high-grade Squamous Intraepithelial Lesion (HSIL) group 20 cases.Immunohistochemistry was performed on the first biopsy tissues and the relative positive ratios of p16, geminin and Ki-67 were calculated.Spearman correlation analysis identified the correlation between the above indicators and the progress of the disease; ROC curve was used to calculate the best diagnostic value of each indicator, and multivariate logistic regression analysis was included to explore the ability of the above indicators to assess the risk of patients progressing to HSIL. \u0000 \u0000 \u0000Results \u0000In the HSIL group, p16 (51.26±17.15)%, geminin relative positive ratio (45.92±15.70) % was higher than those in the LSIL group((43.71±11.84)%, (21.68±14.47)%) and regression group((17.92±9.60)%, (0.16±0.03)%). The difference were statistically significant (F=2.922, 2.751, all P<0.05). Spearman correlation analysis showed that the relative positive ratio of p16 (r=0.27, P=0.014) and geminin (r=0.44, P<0.001) presented a notable positive correlation with the progression of the disease.Under the ROC curve, the best diagnostic values of p16, geminin and Ki-67 were 38.9%, 32.5% and 18.6%, respectively.Multivariate logistic regression analysis showed that the relative positive ratio of p16 was higher than 38.9% (OR=4.366, P=0.006), and geminin was higher than 32.5% (OR=5.392, P=0.011) had a higher risk of progression to HSIL. \u0000 \u0000 \u0000Conclusion \u0000p16 and geminin may be effective biomarkers for identifying patients with advanced LSIL. \u0000 \u0000 \u0000Key words: \u0000p16; Cell cycle factor geminin; Ki-67; Low-grade squamous intraepithelial lesions; High-grade squamous intraepithelial lesions","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45921786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.009
Li Yang, Guojun Wang, Yan Gao, Rong Jing, Kun Huang
Objective To investigate the effect of aerobic exercise combined with Levocarnitine in treating stable angina pectoris(SAP) and its effect on vascular endothelial function and serum levels of superoxide dismutase(SOD), total antioxidant capacity(TAC), glutathione peroxidase(GSH-px), malondialdehyde (MDA), and lipid peroxide(LPO). Methods One hundred and fifty patients with SAP were selected in Affiliated Hospital of Yan′an University from september 2017 to september 2018 and randomly divided into control group and observation group(75 cases in each group). Both groups received routine intervention according to the guidelines.The control group was given with intravenous drip of left carnitine(3 g/time and once a day). On basis of the control group, the observation group was treated with regular aerobic exercise therapy.After 8 weeks' treatment for two groups, attack frequency degree of angina pectoris, score of life quality of Seattle angina scale(SAQ), and clinical effect were compared between the two groups.And the vascular endothelial function(plasma endothelin(ET)1, nitric oxide(NO)), flow mediated vasodilation(FMD) of brachial artery and antioxidant effect(serum levels of SOD, TAC, GSH-px, MDA and LPO) were detected. Results After 8 weeks' treatment, frequency of angina pectoris and episode of duration of angina pectoris in the observation group were less((4.19±0.56) vs.(6.22±0.89) time, (4.31±0.50) vs.(5.25±0.71) min), while life quality indexes SAQ score was higher, than those in the control group, and the difference was statistically significant((18.44±2.30) vs.(12.49±1.82) score, (56.31±6.62) vs.(48.05±5.88) score, (14.46±1.99) vs.(9.22±1.10) score, (21.41±2.95))vs.(18.09±2.26) score, (16.14±2.17) vs.(12.05±1.82) score, (all P<0.01)). The total clinical effective rate of the observation group (92% (69 / 75)) was higher than that of the control group (80% (60 / 75)), the difference was statistically significant (χ2=4.485, P=0.034). After 8 weeks′ treatment, level of ET-1, FMD of brachial artery, MDA, and LPO were lower than those in the control group((65.38±7.91) ng/L vs.(77.12±8.56) ng/L, (9.44±1.34)% vs.(12.55±1.81)%, (2.81±0.43) μmol/L vs.(3.90±0.50) μmol/L, (3.14±0.44) μmol/L vs.(4.40±0.63) μmol/L), while NO, SOD, TAC, GSH-px were higher than control group((67.51±7.79) μmol/L vs.(52.17±6.08) μmol/L, (85.25±9.67)U/L vs.(76.01±8.33) U/L, (12.79±1.80) kU/L vs.(9.64±11.05) kU/L, (117.65±15.03) U/L vs.(111.76±14.19) U/L), and the difference was statistically significant (all P<0.01). Conclusion Aerobic exercise combined with Levocarnitine in treating SAP can reduce the attack of angina pectoris, improve the life treatment of patients, and improve the clinical effect, which may be related to the improvement of endothelial function and antioxidant effect. Key words: Aerobic exercise; Levocarnitine; Stable angina pectoris; Vascular endothelial function; Antioxidant
{"title":"Effects of aerobic exercise combined with Levocarnitine on improving endothelial function and antioxidation in patients with stable angina pectoris of coronary heart disease","authors":"Li Yang, Guojun Wang, Yan Gao, Rong Jing, Kun Huang","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.009","url":null,"abstract":"Objective \u0000To investigate the effect of aerobic exercise combined with Levocarnitine in treating stable angina pectoris(SAP) and its effect on vascular endothelial function and serum levels of superoxide dismutase(SOD), total antioxidant capacity(TAC), glutathione peroxidase(GSH-px), malondialdehyde (MDA), and lipid peroxide(LPO). \u0000 \u0000 \u0000Methods \u0000One hundred and fifty patients with SAP were selected in Affiliated Hospital of Yan′an University from september 2017 to september 2018 and randomly divided into control group and observation group(75 cases in each group). Both groups received routine intervention according to the guidelines.The control group was given with intravenous drip of left carnitine(3 g/time and once a day). On basis of the control group, the observation group was treated with regular aerobic exercise therapy.After 8 weeks' treatment for two groups, attack frequency degree of angina pectoris, score of life quality of Seattle angina scale(SAQ), and clinical effect were compared between the two groups.And the vascular endothelial function(plasma endothelin(ET)1, nitric oxide(NO)), flow mediated vasodilation(FMD) of brachial artery and antioxidant effect(serum levels of SOD, TAC, GSH-px, MDA and LPO) were detected. \u0000 \u0000 \u0000Results \u0000After 8 weeks' treatment, frequency of angina pectoris and episode of duration of angina pectoris in the observation group were less((4.19±0.56) vs.(6.22±0.89) time, (4.31±0.50) vs.(5.25±0.71) min), while life quality indexes SAQ score was higher, than those in the control group, and the difference was statistically significant((18.44±2.30) vs.(12.49±1.82) score, (56.31±6.62) vs.(48.05±5.88) score, (14.46±1.99) vs.(9.22±1.10) score, (21.41±2.95))vs.(18.09±2.26) score, (16.14±2.17) vs.(12.05±1.82) score, (all P<0.01)). The total clinical effective rate of the observation group (92% (69 / 75)) was higher than that of the control group (80% (60 / 75)), the difference was statistically significant (χ2=4.485, P=0.034). After 8 weeks′ treatment, level of ET-1, FMD of brachial artery, MDA, and LPO were lower than those in the control group((65.38±7.91) ng/L vs.(77.12±8.56) ng/L, (9.44±1.34)% vs.(12.55±1.81)%, (2.81±0.43) μmol/L vs.(3.90±0.50) μmol/L, (3.14±0.44) μmol/L vs.(4.40±0.63) μmol/L), while NO, SOD, TAC, GSH-px were higher than control group((67.51±7.79) μmol/L vs.(52.17±6.08) μmol/L, (85.25±9.67)U/L vs.(76.01±8.33) U/L, (12.79±1.80) kU/L vs.(9.64±11.05) kU/L, (117.65±15.03) U/L vs.(111.76±14.19) U/L), and the difference was statistically significant (all P<0.01). \u0000 \u0000 \u0000Conclusion \u0000Aerobic exercise combined with Levocarnitine in treating SAP can reduce the attack of angina pectoris, improve the life treatment of patients, and improve the clinical effect, which may be related to the improvement of endothelial function and antioxidant effect. \u0000 \u0000 \u0000Key words: \u0000Aerobic exercise; Levocarnitine; Stable angina pectoris; Vascular endothelial function; Antioxidant","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47626344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.06.003
Hui-min Guo, Lei Zhu, Jingbo Gao, L. Bai, Zhiyong Sun, Jinli Zhang, Bo Yang, C. Hao
Objective To explore the diagnostic value of flow cytometry in detecting HPV E6/E7 mRNA of human papilloma virus (HPV) in the diagnosis of cervical lesions. Methods From January 2017 to September 2018, 119 women with suspected cervical lesions in the department of gynecology and obstetrics of Shanxi Maternal and Child Health Hospital were selected.Flow cytometry was used to detect HPV E6 / E7 mRNA in cervical exfoliated cells of women, and the DNA of HPV was detected by the method of hybrid capture 2 (HC2). Results 31.09% (37/119) HPV E6/E7 mRNA and 57.14% (68 / 119) HPV DNA were positive in 119 cases.The positive rate of HPV E6/E7 mRNA in cervical intraepithelial neoplasia (CIN)2+ group was 77.78% (28/36), which was statistically significant compared with 20.00% (4/20) in CIN1 group (χ2=15.246, P<0.01), and was statistically significant compared with 7.94% (5/63) in nilm group (χ2=50.286, P<0.01). In nilm group, HPV E6 / E7 mRNA positive rate was 7.94% (5/63) and HPV DNA positive rate was 30.16% (19 / 63), which was statistically significant (χ2=10.088, P=0.001). In cin1 group, HPV E6/ E7 mRNA positive rate was 20.00% (4 / 20) and HPV DNA positive rate was 85.00% (17/20), which was statistically significant (χ2=14.436, P<0.001). The sensitivity, specificity, positive predictive value and negative predictive value of HPV E6 / E7 mRNA detected by flow cytometry were 75.00%, 89.16%, 72.72% and 90.24%, respectively.The sensitivity, specificity, positive predictive value and negative predictive value ofthe detection of HPV DNA by HC2 were 88.89%, 56.63%, 47.06% and 92.16%, respectively. Conclusion The specificity and positive predictive value of HPV E6 / E7 mRNA detected by flow cytometry were better than those detected by HC2.The detection of HPV E6 / E7 mRNA has potential clinical value in cervical precancerous lesions screening. Key words: Cervical intraepithelial neoplasia; Flow cytometry; Papillomavirus family; Papillomavirus E7 Proteins
{"title":"Diagnostic value of flow cytometry in detecting human papillomavirus E6/E7 mRNA in cervical lesions","authors":"Hui-min Guo, Lei Zhu, Jingbo Gao, L. Bai, Zhiyong Sun, Jinli Zhang, Bo Yang, C. Hao","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.06.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.06.003","url":null,"abstract":"Objective \u0000To explore the diagnostic value of flow cytometry in detecting HPV E6/E7 mRNA of human papilloma virus (HPV) in the diagnosis of cervical lesions. \u0000 \u0000 \u0000Methods \u0000From January 2017 to September 2018, 119 women with suspected cervical lesions in the department of gynecology and obstetrics of Shanxi Maternal and Child Health Hospital were selected.Flow cytometry was used to detect HPV E6 / E7 mRNA in cervical exfoliated cells of women, and the DNA of HPV was detected by the method of hybrid capture 2 (HC2). \u0000 \u0000 \u0000Results \u000031.09% (37/119) HPV E6/E7 mRNA and 57.14% (68 / 119) HPV DNA were positive in 119 cases.The positive rate of HPV E6/E7 mRNA in cervical intraepithelial neoplasia (CIN)2+ group was 77.78% (28/36), which was statistically significant compared with 20.00% (4/20) in CIN1 group (χ2=15.246, P<0.01), and was statistically significant compared with 7.94% (5/63) in nilm group (χ2=50.286, P<0.01). In nilm group, HPV E6 / E7 mRNA positive rate was 7.94% (5/63) and HPV DNA positive rate was 30.16% (19 / 63), which was statistically significant (χ2=10.088, P=0.001). In cin1 group, HPV E6/ E7 mRNA positive rate was 20.00% (4 / 20) and HPV DNA positive rate was 85.00% (17/20), which was statistically significant (χ2=14.436, P<0.001). The sensitivity, specificity, positive predictive value and negative predictive value of HPV E6 / E7 mRNA detected by flow cytometry were 75.00%, 89.16%, 72.72% and 90.24%, respectively.The sensitivity, specificity, positive predictive value and negative predictive value ofthe detection of HPV DNA by HC2 were 88.89%, 56.63%, 47.06% and 92.16%, respectively. \u0000 \u0000 \u0000Conclusion \u0000The specificity and positive predictive value of HPV E6 / E7 mRNA detected by flow cytometry were better than those detected by HC2.The detection of HPV E6 / E7 mRNA has potential clinical value in cervical precancerous lesions screening. \u0000 \u0000 \u0000Key words: \u0000Cervical intraepithelial neoplasia; Flow cytometry; Papillomavirus family; Papillomavirus E7 Proteins","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41371778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-01DOI: 10.3760/CMA.J.ISSN.1008-6315.2019.05.004
Shi-Yu Jiang
Objective To analyze the dynamic changes of immunoregulatory factors and tumor markers before and after chemotherapy in patients with advanced gastric cancer. Methods From October 2015 to February 2018, 58 patients with chemotherapy AGC in our hospital were selected as gastric cancer group.According to the efficacy of chemotherapy they were divided into effective group, stable group and ineffective group.Meanwhile, 30 healthy persons were selected as normal group.The immunoglobulins (IgA, IgG, IgM), T lymphocyte subsets (CD3+ , CD4+ , CD8+ , CD4+ /CD8+ ) and related tumor markers (CEA, CA199, CA242) were compared before and after chemotherapy. Results The levels of IgA((2.11±0.89) g/L), IgM((10.65±4.61) g/L), IgG((1.25±0.45) g/L), CD8+ ((28.12±3.56)%), CEA((40.33±16.24) μg/L), CA199((76.34±21.56) kU/L) and CA242((29.34±9.57)k U/L) in the gastric cancer group were significantly higher than those in the normal group IgA((0.93±0.36) g/L), IgM((6.46±3.59) g/L), IgG((0.65±0.32) g/L), CD8+ ((25.02±4.78)%), CEA((1.81±0.55) μg/L), CA199((7.51±2.67) kU/L), CA242((3.35±1.21) kU/L)(t=6.958, 3.600, 6.495, 3.435, 12.952, 17.370, 14.773, P<0.05), while CD3+ ((64.12±5.12)%), CD4+ ((34.12±4.10)%), CD4+ /CD8+ (1.09±0.28) were lower than the normal group (CD3+ (71.23±7.14)%, CD4+ (39.78±5.20)%, CD4+ /CD8+ (1.47±0.40))(t=5.376, 5.592, 5.192, P<0.05). The total effective rate of AGC patients in gastric cancer group was 79.31 % (46/58), and the ineffective rate was 20.69 % (12/58). IgA, IgG, IgM, and CD8+ in the effective group were significantly lower than those before chemotherapy (t=3.925, 3.745, 4.036, 2.661, P<0.05), while CD3+ , CD4+ , CD4+ /CD8+ were significantly higher than before chemotherapy (t=3.520, 3.077, 3.218, P<0.05). The subcellular level of protein and T lymphocytes was significantly better than that of stable group and ineffective group (P<0.05). The levels of tumor markers CEA, CA199 and CA242 in the effective group and stable group were significantly lower than those before chemotherapy (P<0.05), and significantly lower than the ineffective group (P<0.05). Conclusion The levels of immunoglobulins, T lymphocyte subsets and tumor markers in patients with AGC have significant changes after chemotherapy, and their levels can guide the efficacy of chemotherapy. Key words: Advanced gastric cancer; Chemotherapy; Immunoglobulin; T lymphocyte subsets; Tumor markers
{"title":"Dynamic study of immunoregulatory factors and tumor markers in patients with advanced gastric cancer before and after chemotherapy","authors":"Shi-Yu Jiang","doi":"10.3760/CMA.J.ISSN.1008-6315.2019.05.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-6315.2019.05.004","url":null,"abstract":"Objective \u0000To analyze the dynamic changes of immunoregulatory factors and tumor markers before and after chemotherapy in patients with advanced gastric cancer. \u0000 \u0000 \u0000Methods \u0000From October 2015 to February 2018, 58 patients with chemotherapy AGC in our hospital were selected as gastric cancer group.According to the efficacy of chemotherapy they were divided into effective group, stable group and ineffective group.Meanwhile, 30 healthy persons were selected as normal group.The immunoglobulins (IgA, IgG, IgM), T lymphocyte subsets (CD3+ , CD4+ , CD8+ , CD4+ /CD8+ ) and related tumor markers (CEA, CA199, CA242) were compared before and after chemotherapy. \u0000 \u0000 \u0000Results \u0000The levels of IgA((2.11±0.89) g/L), IgM((10.65±4.61) g/L), IgG((1.25±0.45) g/L), CD8+ ((28.12±3.56)%), CEA((40.33±16.24) μg/L), CA199((76.34±21.56) kU/L) and CA242((29.34±9.57)k U/L) in the gastric cancer group were significantly higher than those in the normal group IgA((0.93±0.36) g/L), IgM((6.46±3.59) g/L), IgG((0.65±0.32) g/L), CD8+ ((25.02±4.78)%), CEA((1.81±0.55) μg/L), CA199((7.51±2.67) kU/L), CA242((3.35±1.21) kU/L)(t=6.958, 3.600, 6.495, 3.435, 12.952, 17.370, 14.773, P<0.05), while CD3+ ((64.12±5.12)%), CD4+ ((34.12±4.10)%), CD4+ /CD8+ (1.09±0.28) were lower than the normal group (CD3+ (71.23±7.14)%, CD4+ (39.78±5.20)%, CD4+ /CD8+ (1.47±0.40))(t=5.376, 5.592, 5.192, P<0.05). The total effective rate of AGC patients in gastric cancer group was 79.31 % (46/58), and the ineffective rate was 20.69 % (12/58). IgA, IgG, IgM, and CD8+ in the effective group were significantly lower than those before chemotherapy (t=3.925, 3.745, 4.036, 2.661, P<0.05), while CD3+ , CD4+ , CD4+ /CD8+ were significantly higher than before chemotherapy (t=3.520, 3.077, 3.218, P<0.05). The subcellular level of protein and T lymphocytes was significantly better than that of stable group and ineffective group (P<0.05). The levels of tumor markers CEA, CA199 and CA242 in the effective group and stable group were significantly lower than those before chemotherapy (P<0.05), and significantly lower than the ineffective group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The levels of immunoglobulins, T lymphocyte subsets and tumor markers in patients with AGC have significant changes after chemotherapy, and their levels can guide the efficacy of chemotherapy. \u0000 \u0000 \u0000Key words: \u0000Advanced gastric cancer; Chemotherapy; Immunoglobulin; T lymphocyte subsets; Tumor markers","PeriodicalId":10365,"journal":{"name":"Clinical Medicine of China","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49361846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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