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Knowledge, Attitude, and Perceived Harm of e-Cigarette Use Behaviour among Medical and Dental Undergraduate Students in UiTM 南京医科大学医学与牙科本科学生电子烟使用行为的知识、态度与感知危害
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19235
MF Mahamad Sobri, A. Azlan, NF Md. Bohari, Nawwal Alwani Mohd Radzi, NN Bakri
Background: Healthcare professionals have a prominent role to play in addressing the tobacco epidemic and the rapid increase of e-cigarette use in the population. The growing interest of electronic cigarettes has led to a broad debate whether e-cigarettes can be used as a harm reduction tool towards smoking. Objectives: This study aims to explore the knowledge, attitude, and perceived harm of vaping behaviour among medical and dental undergraduate students in UiTM. Methods: A questionnaire-based survey was administered using Google Form to all participants. Demographics and data from four domains of knowledge, attitude towards vaping behaviour, mass media exposure of e-cigarettes and perception of health-related risks of vaping were collected. Results: Data were obtained from 309 undergraduates with a mean age of 20.6 (SD ± 1.60). The majority of them have never smoked (96.1%) or used e-cigarettes (99.0%). Nine in 10 believe they play a role in their patient’s smoking cessation therapy in the future (91.3%). Slightly more than half (62.1%) have poor knowledge of e-cigarette, less favourable attitude towards vaping (50.8%) and perceived e-cigarettes to be less harmful (56.0%). The majority of them also claimed high exposure towards e-cigarette marketing from the mass media (85.1%). Conclusion: UiTM medical and dental undergraduates have less favourable attitudes towards e-cigarettes but lacked knowledge on the issue. They also perceived the e-cigarettes to be less harmful to health compared to conventional cigarettes. This points out the urgent need to further educate health professionals and also to provide clear guidelines at every level to regulate vaping behaviour among the population.
背景:医疗保健专业人员在解决烟草流行和人口中电子烟使用迅速增加方面发挥着突出作用。人们对电子烟的兴趣日益浓厚,这引发了一场广泛的辩论,即电子烟是否可以作为一种减少吸烟危害的工具。目的:本研究旨在了解南京医科大学医学和牙科专业本科生对电子烟的认知、态度和感知危害。方法:采用谷歌表格对所有参与者进行问卷调查。收集了来自四个知识领域的人口统计数据和数据,包括对电子烟行为的态度、大众媒体对电子烟的接触以及对电子烟健康风险的认识。结果:数据来自309名大学生,平均年龄20.6岁(SD±1.60)。他们中的大多数从未吸烟(96.1%)或使用电子烟(99.0%)。十分之九的人认为他们在病人未来的戒烟治疗中发挥作用(91.3%)。略多于一半(62.1%)的人对电子烟知之甚少,对电子烟的态度不太好(50.8%),认为电子烟的危害较小(56.0%)。他们中的大多数人还声称大众媒体对电子烟营销的曝光率很高(85.1%)。结论:UiTM医学和牙科专业大学生对电子烟的态度较差,但缺乏相关知识。他们还认为,与传统香烟相比,电子烟对健康的危害更小。这表明迫切需要进一步教育卫生专业人员,并在各级提供明确的指导方针,以规范人群中的电子烟行为。
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引用次数: 1
The Therapeutic Potential of Plant Extraction in Oral Health - A Systematic Review 植物提取物在口腔健康中的治疗潜力——系统综述
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19237
F. Mohd Fauzi, AM Mischon, N. M. Mohamad Zain, IH Baharuddin
Objectives: This study aimed to analyze the therapeutic potential of plant extract against oral microorganisms in published literature. Material and Method: A systematic literature review was performed through electronic databases (Scopus, EBSCO: Dentistry and Oral Science Source) from January 2009 till December 2019 with the search terms (“extract” AND (“ORAL MICROORGANISM” OR “ORAL BACTERIA”) AND (“stem” OR “Bark” OR “Leaf”)). Results: Out of 409 articles, 21 articles met our inclusion criteria that were subjected to data extraction and review. The data disclosed antimicrobial and antibiofilm activity of plant extracts against causative microorganisms of caries, periodontitis, endodontic infection, and fungal infection. Ethanol was the most common solvent used for plant extraction. The antimicrobial test was reported in all studies using different methodologies such as minimum inhibitory concentration (MIC), disk diffusion method, agar well method, intracanal irrigation, and Fractional Inhibitory Concentration Index (FICI) . None of the plant extract tested showed significant toxicity in five studies that conducted toxicity assays. Conclusion: A positive correlation was observed between plant extract and antimicrobial activity against oral microorganisms. In that context, integrating plant extract in oral healthcare products could be an option to enhance effective antimicrobial control. However, further clinical studies are required to provide clinical evidence to support these observations.
目的:分析已发表文献中植物提取物对口腔微生物的治疗潜力。材料和方法:从2009年1月到2019年12月,通过电子数据库(Scopus, EBSCO: Dentistry and Oral Science Source)进行系统的文献综述,检索词为(“提取物”、(“口腔微生物”或“口腔细菌”)和(“茎”、“树皮”或“叶子”)。结果:在409篇文章中,21篇文章符合我们的纳入标准,我们进行了数据提取和审查。这些数据揭示了植物提取物对龋齿、牙周炎、牙髓感染和真菌感染的病原微生物的抗菌和抗生物膜活性。乙醇是植物提取中最常用的溶剂。所有的研究都采用不同的方法进行抗菌试验,如最低抑菌浓度(MIC)、磁盘扩散法、琼脂孔法、管内冲洗法和分数抑菌浓度指数(FICI)。在进行毒性分析的五项研究中,没有一项植物提取物显示出明显的毒性。结论:植物提取物与口腔微生物抑菌活性呈正相关。在这种情况下,将植物提取物整合到口腔保健产品中可能是加强有效抗菌控制的一种选择。然而,需要进一步的临床研究来提供临床证据来支持这些观察结果。
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引用次数: 1
In-vitro Comparison of Antibacterial Activities on Stingless Bee Propolis using Selected Extraction Methods 不同提取方法对无刺蜂胶抑菌活性的体外比较
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19230
Ohh Al-Masoodi, HB Said Gulam Khan, IH Baharuddin, IH Ismail
This study aimed to compare the different selected extraction methods of propolis extracts against Enterococcus faecalis (E. faecalis). Extraction of Malaysian Propolis (MP) from Heterotrigona itama, was carried out using 70% ethanol. For the selected extraction processes, such as, centrifugation-assisted extraction (CAE), vacuum-assisted extraction (VAE), and shaking-assisted extraction (SAE) methods were used. Antimicrobial activity against E. faecalis was assessed using the antibacterial susceptibility test (AST). The results showed that SAE, followed by CAE, had better antimicrobial properties as compared to those obtained by VAE. The inhibition zones for SAE, CAE, and VAE were 5.3mm, 4.67mm, and 4.16mm, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for extracted MP by SAE were 12.5 and 25 mg/mL, respectively. Furthermore, non-setting calcium hydroxide’s [Ca(OH)2] MIC and MBC were determined to be 50 and 100 mg/mL, respectively. As a result, we believe that extraction of raw MP from Heterotrigona itama using the SAE method was more effective than Ca(OH)2 against E. faecalis.
本研究旨在比较蜂胶提取物对粪肠球菌(E. faecalis)的不同提取方法。以70%乙醇为萃取剂,对马来蜂胶进行了提取。选取了离心辅助提取(CAE)、真空辅助提取(VAE)和摇振辅助提取(SAE)等提取工艺。采用抗菌药敏试验(AST)评价其对粪肠杆菌的抑菌活性。结果表明,与VAE相比,SAE的抗菌性能更好,其次是CAE。SAE、CAE和VAE的抑制区分别为5.3mm、4.67mm和4.16mm。SAE提取的MP最低抑菌浓度(MIC)为12.5 mg/mL,最低杀菌浓度(MBC)为25 mg/mL。测定了不凝固氢氧化钙[Ca(OH)2] MIC和MBC分别为50和100 mg/mL。因此,我们认为用SAE法提取粗MP比Ca(OH)2对粪肠杆菌更有效。
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引用次数: 2
Formulation & Evaluation of Myrrh Toothpaste 没药牙膏的配方及评价
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19227
NH Abd Majid, RS Abdul Rahim, LT Younis, MI Abu Hassan
Objectives: To formulate and measure the cytotoxicity level of organic myrrh toothpaste. Materials and Methods: Myrrh extract was prepared by the freeze drying process. Toothpaste was formulated by mixing specified amounts of myrrh extract, hydroxypropyl methylcellulose (HPMC) polymer, sodium lauryl sulphate (SLS), mint, and sucralose with deionized distilled water. MTT test was performed using concentrations of 100, 200, 300 and 400 mg/ml to assess the effect of myrrh paste on the gingival fibroblasts viability at intervals of 24 and 48hrs. The results were analysed by using SPSS version 27. Results: The formulated myrrh toothpaste has a homogeneous consistency as the extracted myrrh successfully dissolved completely with the other components. Cell viability test showed that myrrh paste concentrations of 100 - 300mg/ml were effective in maintaining the rate of fibroblasts growth after 24 and 48 hours as compared to the control samples. Cell growth rate was suppressed in the test samples treated with paste concentration of 400mg/ml. The results of the study imply that 300mg/ml is the safe and optimal concentration for fibroblasts growth, whereas concentrations ≥ 400mg/ml are intolerable and might be suppressing the proliferation of fibroblasts. Conclusion: Myrrh toothpaste concentration at 300mg/ml is the safe and optimal concentration for fibroblasts growth as depicted in the results, suggesting that at the optimal formulation, myrrh extract may not be toxic to the soft tissues and myrrh toothpaste may be useful for oral health care. Further clinical investigations are recommended to obtain the clinical efficacy data of the organic myrrh toothpaste.
目的:研制并测定有机没药牙膏的细胞毒性水平。材料与方法:采用冷冻干燥法制备没药提取物。牙膏是通过将一定量的没药提取物、羟丙基甲基纤维素(HPMC)聚合物、月桂基硫酸钠(SLS)、薄荷和三氯蔗糖与去离子蒸馏水混合而成的。采用100、200、300、400 mg/ml浓度的MTT试验,隔24、48h观察没药糊剂对牙龈成纤维细胞活力的影响。采用SPSS 27版对结果进行分析。结果:所制没药牙膏具有均匀的稠度,所提取的没药与其它成分完全溶解。细胞活力测试表明,与对照样品相比,100 - 300mg/ml没药膏浓度在24和48小时后能有效维持成纤维细胞的生长速度。膏体浓度为400mg/ml时,细胞生长速率受到抑制。研究结果表明,300mg/ml是成纤维细胞生长的安全和最佳浓度,而≥400mg/ml的浓度是不可忍受的,可能会抑制成纤维细胞的增殖。结论:300mg/ml没药牙膏浓度为成纤维细胞生长的安全、最佳浓度,提示在最佳配方下,没药提取物可能对软组织无毒,没药牙膏可能具有口腔保健作用。建议进一步的临床研究,以获得有机没药牙膏的临床疗效数据。
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引用次数: 0
Occlusal Considerations and Multidisciplinary Approach in the Rehabilitation of Generalised Tooth Surface Loss 全身性牙面缺损修复的咬合考虑和多学科方法
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19238
Eleena Mohd Yusof
Tooth wear is prevalent in the society and often-time affects an individual’s daily life. This case demonstrates the management of non-carious tooth surface loss with loss of occlusal vertical dimension using multidisciplinary approach involving periodontics, endodontics and prosthodontics. The tooth surface loss was graded moderate to severe anteriorly and affecting the patient’s overall appearance and self-confidence. Rehabilitation of this case involves restoring the anterior teeth at an increased occlusal vertical dimension, using reorganised approach and allowing relative axial tooth movement posteriorly. Clinical Relevance: The author believes the understanding of available conservative treatment options, occlusion concepts, proper material handling and satisfactory communication with other disciplines and the laboratory aid a clinician in achieving the best treatment outcome and better patient satisfaction.
牙齿磨损在社会上很普遍,而且经常影响到个人的日常生活。本病例展示了使用包括牙周学、牙髓学和修复学在内的多学科方法治疗非龋齿牙面缺失和咬合垂直尺寸缺失。牙面脱落前分级为中度至重度,影响患者的整体外观和自信心。该病例的康复包括在增加的咬合垂直尺寸上恢复前牙,使用重组入路并允许相对轴向的后牙运动。临床意义:作者认为了解可用的保守治疗方案、闭塞概念、正确的材料处理以及与其他学科和实验室的良好沟通有助于临床医生获得最佳治疗结果和更好的患者满意度。
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引用次数: 0
Self-Reported Prevalence of Recurrent Aphthous Stomatitis among UiTM Dental Students UiTM牙科学生口疮性口腔炎复发的自我报告患病率
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19233
WN Wan Ahmad Kamil, IQ Amirham, M. Omar, M. Zainal
Recurrent aphthous stomatitis (RAS) is a common mucosal disease characterized by multiple recurrent, small, round, or oval ulcers with circumscribed margins, erythematous haloes, and yellow grey base. Although the exact etiology is unknown, various factors can trigger RAS development. Objectives: This study aims to determine the prevalence of self-reported RAS and to evaluate the associated risk factors of RAS among dental students. Material and methods: A set of validated questionnaires was distributed among the dental students in the Faculty of Dentistry, UiTM. The questionnaire comprises socio-demographic characteristics, nature of ulcers, risk factors, and treatment modalities. Data obtained from the questionnaire were analyzed using Chi-square statistical test SPSS version 27. Results: A total of 299 dental students participated in the questionnaire survey. The prevalence of RAS among dental students was 90.3 %. There was no significant relationship between the socio-demographic background of participants with self-reported RAS. The most common site affected was the buccal mucosa 64.1%, followed by the lips 55.2%. Risk factors frequently associated with RAS are injury (trauma) and stress 47.8% and 28.1%, respectively. Most dental students (69.3%) do not get any treatment to treat their ulcers, and only 1.5% seek treatment from the dentist. Conclusions: This study highlights the high prevalence of self-reported RAS among dental students and common risk factors reported associated with the occurrence of RAS. Therefore, this data assists the clinician in identifying common associated risk factors of RAS and subsequently improve management and efficient therapeutics.
复发性口疮性口炎(RAS)是一种常见的粘膜疾病,其特征是多发性复发,小的,圆形的或椭圆形的溃疡,边缘有边界,红斑晕,基底呈黄色灰色。虽然确切的病因尚不清楚,但多种因素可引发RAS的发展。目的:本研究旨在了解牙科学生自我报告的RAS患病率,并评估RAS的相关危险因素。材料与方法:采用一套有效的问卷调查方法,对南京中医药大学牙科学院的牙科学生进行问卷调查。调查问卷包括社会人口学特征、溃疡的性质、危险因素和治疗方式。问卷数据采用SPSS 27版卡方统计检验进行分析。结果:共有299名牙科学生参与问卷调查。口腔学生中RAS患病率为90.3%。参与者的社会人口背景与自我报告的RAS之间没有显著的关系。最常见的受累部位为颊黏膜64.1%,其次为唇部55.2%。经常与RAS相关的危险因素是损伤(创伤)和压力,分别为47.8%和28.1%。大多数牙科学生(69.3%)没有接受任何治疗溃疡的治疗,只有1.5%向牙医寻求治疗。结论:本研究强调了牙科学生中自我报告的RAS的高患病率以及与RAS发生相关的常见危险因素。因此,这些数据有助于临床医生识别RAS的常见相关危险因素,并随后改善管理和有效的治疗方法。
{"title":"Self-Reported Prevalence of Recurrent Aphthous Stomatitis among UiTM Dental Students","authors":"WN Wan Ahmad Kamil, IQ Amirham, M. Omar, M. Zainal","doi":"10.24191/cos.v9i2.19233","DOIUrl":"https://doi.org/10.24191/cos.v9i2.19233","url":null,"abstract":"Recurrent aphthous stomatitis (RAS) is a common mucosal disease characterized by multiple recurrent, small, round, or oval ulcers with circumscribed margins, erythematous haloes, and yellow grey base. Although the exact etiology is unknown, various factors can trigger RAS development. Objectives: This study aims to determine the prevalence of self-reported RAS and to evaluate the associated risk factors of RAS among dental students. Material and methods: A set of validated questionnaires was distributed among the dental students in the Faculty of Dentistry, UiTM. The questionnaire comprises socio-demographic characteristics, nature of ulcers, risk factors, and treatment modalities. Data obtained from the questionnaire were analyzed using Chi-square statistical test SPSS version 27. Results: A total of 299 dental students participated in the questionnaire survey. The prevalence of RAS among dental students was 90.3 %. There was no significant relationship between the socio-demographic background of participants with self-reported RAS. The most common site affected was the buccal mucosa 64.1%, followed by the lips 55.2%. Risk factors frequently associated with RAS are injury (trauma) and stress 47.8% and 28.1%, respectively. Most dental students (69.3%) do not get any treatment to treat their ulcers, and only 1.5% seek treatment from the dentist. Conclusions: This study highlights the high prevalence of self-reported RAS among dental students and common risk factors reported associated with the occurrence of RAS. Therefore, this data assists the clinician in identifying common associated risk factors of RAS and subsequently improve management and efficient therapeutics.","PeriodicalId":10525,"journal":{"name":"Compendium of Oral Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90845576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Holdaway Soft Tissue Cephalometric Standard for Malay Adults 马来成人霍德威软组织头颅测量标准
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19231
IY Noviaranny, NK Hissam, Pua Abu Bakar
Objectives: The aim of this study were: 1. To report facial soft tissue measurements in Class I Malay adult, 2. To compare between gender, 3. And to check applicability of Holdaway values for Class I Malay adults. Materials and Methods: Lateral cephalometric of 20 men and 20 women age 20-30 years with Class I skeletal was selected from Orthodontic Clinic, Faculty of Dentistry UiTM. The subject has no orthodontic or orthognathic surgery done before the radiograph was taken. The radiograph was traced and evaluated by the authors. Cephalometric landmarks were located according to Holdaway analysis. Ten linear and two angular measurements were produced on each radiograph and measured. For each variable, the mean and standard deviation (SD) was calculated using simple t-test (p<0.05). Results: Soft tissue measurements for Malay have significant difference as compared to Holdaway value except for three parameters which are soft tissue facial angle, superior sulcus depth, and soft tissue chin thickness. When compared between genders, male has larger value in skeletal profile convexity, upper lip strain and soft tissue thickness while females demonstrated higher value in nose prominence and superior sulcus depth. Conclusions: It has been concluded that Malay values for Holdaway soft tissue analysis were found to be statistically different. Hence Holdaway soft tissue values are not applicable for use in orthodontic practice for Malay population. Further research to confirm values obtained for Malay population would aid in better diagnosis and treatment planning.
目的:本研究的目的是:1。报告马来成人I级面部软组织测量,2。3、性别之间的比较;并检验霍德威值在马来成人一级的适用性。材料与方法:选择20名男性和20名女性,年龄20-30岁,骨性I类患者。受试者在拍摄x光片之前没有做过正畸或正颌手术。作者对x线片进行了追踪和评估。根据Holdaway分析定位头颅测量标志。在每张x光片上进行了10次直线测量和2次角度测量。采用简单t检验计算各变量的均值和标准差(SD) (p<0.05)。结果:马来人的软组织测量值除软组织面部角度、上沟深度和软组织下巴厚度三个参数外,与Holdaway值有显著差异。从性别上比较,男性在骨骼轮廓凸度、上唇张力和软组织厚度上的值较大,而女性在鼻突和上沟深度上的值较高。结论:马来语值对霍德威软组织分析有统计学差异。因此,霍德威软组织值不适用于马来人口的正畸实践。进一步的研究以确认马来人口的价值,将有助于更好的诊断和治疗计划。
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引用次数: 1
Shape Visualization of Dental Inclination in Class I and Bimaxillary Protrusion Malocclusions through Geometric Morphometric Analysis 基于几何形态计量学分析的ⅰ类和双颌前突错颌牙倾斜形态可视化
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19236
Siti Balqish Oon, Aspalilah Alias, Sarah Haniza, Abdul Ghani, Noraina Hafizan
Objectives: This study aimed to determine the shape of dental inclination between Class I malocclusion and bimaxillary protrusive Malay patients using geometric morphometric analysis obtained from lateral cephalometric radiographs. Materials and Methods: 128 Malay patients who met the inclusion criteria were recruited; of which 64 patients were in Class I malocclusion and another 64 patients in bimaxillary protrusion groups. Samples were in the age range of 18 to 40 years old, with a gender distribution of 41 males and 87 females. Pre-treatment orthodontic lateral cephalometric radiograph were traced and digitized. Thirteen landmarks of each radiograph were incorporated and converted into coordinates using TPSUtil software. The plots were then exported to MorphoJ Software for multiple Geometric Morphometric Analysis (GMA). Results: The results showed that there was a statistically significant difference (p<0.01) in shape of dental inclination between the groups. Discriminant analysis and cross validation of these groups demonstrated 98% accuracy in identifying two types of dental malocclusion. The grid graphs illustrated the shape of dental inclination of the bimaxillary protrusion group as being significantly more proclined when compared to Class I malocclusion. Conclusion: This study concludes that the dissimilarities in dental inclination are identifiable in Class I malocclusion and bimaxillary protrusion and the shape differences can be visualized using geometric morphometric analysis.
目的:本研究旨在通过侧位头颅x线片的几何形态分析来确定I类错颌和双颌前突患者的牙倾斜形状。材料与方法:招募符合纳入标准的128名马来患者;其中ⅰ类错颌合组64例,双颌前突组64例。样本年龄在18 - 40岁之间,性别分布为男性41人,女性87人。对治疗前正畸侧位头颅x线片进行追踪和数字化。利用TPSUtil软件将每张x光片的13个地标合并并转换为坐标。然后将这些图导出到MorphoJ软件进行多重几何形态分析(GMA)。结果:两组间牙倾形状差异有统计学意义(p<0.01)。这些组的判别分析和交叉验证表明,识别两种牙错的准确率为98%。网格图显示,双颌前突组的牙倾斜形状与I类错牙合相比明显更前倾。结论:ⅰ类错颌和双颌前突的牙倾差异是可以识别的,其形状差异可以通过几何形态计量学分析可视化。
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引用次数: 0
Fructus mume Aqueous Extract's Antibacterial Effect on Multi-species Biofilm 乌梅水提物对多种生物膜的抑菌作用
Pub Date : 2022-09-01 DOI: 10.24191/cos.v9i2.19225
Aaeid Ayoub, G. Cheung
Aim: The purpose of this study was to determine the efficacy of several irrigants and an aqueous extract of Fructus mume in eradicating three kinds of biofilm. Materials and Methods: A three-species biofilm of E. faecalis, P. gingivalis and F. nucleatum were cultivated for one week on Thermanox™ plates. Each specimen was immersed for 8 minutes in various irrigants: Fructus mume solution, buffered citric acid, sodium hypochlorite and control (non-irrigated). LIVE/DEAD® BacLightTM staining and confocal light scanning microscopy were used to determine the number of viable bacteria left on the substrate. The same biofilm was then extracted and subjected to scanning electron microscopy (SEM). Results: Images were taken at 12 locations across the biofilm, which were then classified into four areas of concern: Bottom, which would spend the most of the experiment submerged in the solution; It was struck in the centre by the stream of irrigant.; Middle and Upper, where the impression was caused by the irrigant splattering or vaporizing. Results of the amount of viable bacteria residual indicated that Fructus mume showed no significant activity, with an effect like control and even buffered citric acid, and significantly inferior to sodium hypochlorite. Conclusion: Sodium hypochlorite (0.5%) solution was greater to buffered citric acid, Fructus mume and control group as an antimicrobial agent against three-species biofilm.
目的:研究几种灌洗剂和一种乌梅水提物对三种生物膜的清除效果。材料与方法:在Thermanox™板上培养粪肠杆菌、牙龈卟啉卟啉杆菌和核梭菌三种生物膜,培养一周。每个标本在不同的冲洗液中浸泡8分钟:枸杞溶液、缓冲柠檬酸、次氯酸钠和对照(非冲洗)。使用LIVE/DEAD®BacLightTM染色和共聚焦光扫描显微镜测定底物上剩余活菌的数量。然后提取相同的生物膜并进行扫描电子显微镜(SEM)。结果:在生物膜上的12个位置拍摄了图像,然后将其分为四个关注区域:底部,将在溶液中度过大部分实验时间;它的中心被灌溉水击中了。中部和上部,在那里的印象是由喷剂飞溅或蒸发造成的。活菌残留量测定结果表明,乌梅的活性不显著,与对照甚至缓冲柠檬酸的作用相当,明显不如次氯酸钠。结论:次氯酸钠(0.5%)溶液对三种生物膜的抑菌效果优于缓冲柠檬酸、枸杞子和对照组。
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引用次数: 0
The Effect of Different Milk Products on Enamel Hardness: An In Vitro Study 不同乳制品对牙釉质硬度影响的体外研究
Pub Date : 2022-01-13 DOI: 10.24191/cos.v9i1.16909
HB Said Gulam Khan, NI Mohd Sarmin, MF Zahid, N. Hashim
Objectives: An in vitro study to assess the effect of different milk products on the hardness of enamel surfaces. Materials and Methods: Extracted primary and permanent teeth incisors (15 deciduous and 15 permanent) were collected and cleaned. The extracted teeth were then divided randomly into five groups (n=6 per group): G1- distilled water (control); G2- fresh milk (cow milk); G3- chocolate flavoured milk; G4- orange flavoured cultured milk; G5 - fruit lassi milk. The 10 days immersion cycles for the test products were performed thrice daily and were interspersed with exposure of the artificial saliva. Measurement of microhardness on the enamel surface microhardness measurement were performed at baseline, 5- and 10- day of experimentation using Vickers hardness test machine. The pH of each milk products was also assessed. The data were evaluated with repeated measures ANOVA test. Results: Group 4 had significant reduction in microhardness (p<0.05) compared to the control group while Group 1 showed significant reduction (p<0.05) in microhardness compared to all tested samples for day 5 and day 10. While for intergroup comparison, there was no difference in group 3 and group 2. However, there were significant difference between group 4 and group 5 with all other groups on day 10 (p<0.05). Conclusion: Fresh milk showed to be more effective in increasing the enamel microhardness meanwhile orange culture milk has the highest reduction of enamel surface hardness. Thus, we suggest that milk product with low pH has high tendency in enamel erosion.
目的:通过体外实验研究不同乳制品对牙釉质表面硬度的影响。材料与方法:收集拔出的乳牙和恒切牙(15颗乳牙和15颗恒切牙)并进行清洁。拔牙后随机分为5组(每组n=6): G1-蒸馏水组(对照组);G2-鲜奶(牛奶);G3-巧克力味牛奶;G4-橙味培养乳;G5 -水果牛奶。测试产品的10天浸泡周期每天进行三次,并穿插接触人工唾液。采用维氏硬度试验机分别在实验第5天、第10天、第4天对牙釉质表面进行显微硬度测量。还对每种奶制品的pH值进行了评估。采用重复测量方差分析对资料进行评价。结果:第4组与对照组相比,显微硬度显著降低(p<0.05);第1组在第5天、第10天与所有测试样品相比,显微硬度显著降低(p<0.05)。组间比较,3组与2组无差异。第10天,4、5组与其他各组比较差异均有统计学意义(p<0.05)。结论:鲜奶对牙釉质显微硬度的提高效果最好,而橙培养奶对牙釉质表面硬度的降低效果最好。因此,我们认为低pH值的乳制品具有较高的牙釉质侵蚀倾向。
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引用次数: 0
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Compendium of Oral Science
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