Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1166
S. A. G. Pinto, Ana Carla Aparicio, Jefferson Souza, I. Suffredini, S. Cartwright, L. Bonamin
Introduction: Solvatochromic dyes are probes to detect variations on the dipole moment of solvents after the insertion of homeopathic potencies. Recent studies have shown they can be useful tools in laboratory and field studies to detect the activity of homeopathic remedies. Objective: Determine whether solvatochromic dyes can be a diagnostic tool for cells infected by different agents and/or markers to identify the activity of homeopathic medicines. Methods: Ethilicum 1cH, Silicea terra 6, 30, 200cH; Zincum metallicum 6, 30, 200cH and Phosphorus 6, 30 and 200cH were analyzed by pouring the samples (in a 1:60 rate) into a series of seven dyes (rhodamine, ET 33, ET 30, coumarin 7, NN DMIA, Nile red, methylene violet) diluted in absolute ethanol using pre-established working concentrations. Oscillations of dye absorbance were observed at visible light spectrophotometry according to the remedy and potency. Water and succussed water were used as controls. In a second moment, the absorbance profile of the remedies will be compared with those of biological samples (supernatants) and checked with the biological effect previously obtained from each treatment. Supernatants of RAW 264.7 macrophages stimulated by Calmette-Guérin bacilli (BCG) or infected with Encephalitozoon cuniculi will be analyzed. Results: Preliminary results have shown that Silicea terra 6cH, Phosphorus 30 and 200cH and Zincum metallicum 6, 30 and 200cH reduced the absorbance of methylene violet (p=0.01). Repetitions and analysis of supernatants are expected to be performed in the next steps of the study. Future perspectives: Establish a pattern of reactivity of the studied medicines with different dyes and the putative relation with the corresponding supernatants, as an attempt to obtain a “physicochemical signature” for each kind of infection and/or treatment.
介绍:溶剂致变色染料是探针，用于检测插入顺势疗法后溶剂偶极矩的变化。最近的研究表明，在实验室和实地研究中，它们可以成为检测顺势疗法药物活性的有用工具。目的:确定溶剂致变色染料是否可以作为被不同药物和/或标记物感染的细胞的诊断工具，以鉴定顺势疗法药物的活性。方法:Ethilicum 1cH, silica terra 6,30,200ch;将样品以1:60的比例倒入7种染料中(罗丹明、ET 33、ET 30、香豆素7、NN DMIA、尼罗河红、亚甲基紫)，用预先设定的工作浓度在无水乙醇中稀释，分析金属锌6、30、200cH和磷6、30和200cH。在可见光分光光度法下观察染料的吸光度随药物和药效的变化而变化。以白开水和豆浆水为对照。在第二时刻，药物的吸光度曲线将与生物样品(上清液)的吸光度曲线进行比较，并与以前从每次处理中获得的生物效应进行核对。用卡介苗(卡介苗)刺激的RAW 264.7巨噬细胞上清液和感染的ccuphalitozoon进行分析。结果:初步结果表明，硅土6cH、磷30和200cH以及金属锌6、30和200cH降低了亚甲基紫的吸光度(p=0.01)。上清的重复和分析预计将在研究的下一步进行。未来展望:建立所研究药物与不同染料的反应性模式以及与相应上清的推定关系，试图获得每种感染和/或治疗的“物理化学特征”。
{"title":"Characterization of physicochemical markers for homeopathic medicines and biological supernatant samples","authors":"S. A. G. Pinto, Ana Carla Aparicio, Jefferson Souza, I. Suffredini, S. Cartwright, L. Bonamin","doi":"10.51910/ijhdr.v21i1.1166","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1166","url":null,"abstract":"Introduction: Solvatochromic dyes are probes to detect variations on the dipole moment of solvents after the insertion of homeopathic potencies. Recent studies have shown they can be useful tools in laboratory and field studies to detect the activity of homeopathic remedies. Objective: Determine whether solvatochromic dyes can be a diagnostic tool for cells infected by different agents and/or markers to identify the activity of homeopathic medicines. Methods: Ethilicum 1cH, Silicea terra 6, 30, 200cH; Zincum metallicum 6, 30, 200cH and Phosphorus 6, 30 and 200cH were analyzed by pouring the samples (in a 1:60 rate) into a series of seven dyes (rhodamine, ET 33, ET 30, coumarin 7, NN DMIA, Nile red, methylene violet) diluted in absolute ethanol using pre-established working concentrations. Oscillations of dye absorbance were observed at visible light spectrophotometry according to the remedy and potency. Water and succussed water were used as controls. In a second moment, the absorbance profile of the remedies will be compared with those of biological samples (supernatants) and checked with the biological effect previously obtained from each treatment. Supernatants of RAW 264.7 macrophages stimulated by Calmette-Guérin bacilli (BCG) or infected with Encephalitozoon cuniculi will be analyzed. Results: Preliminary results have shown that Silicea terra 6cH, Phosphorus 30 and 200cH and Zincum metallicum 6, 30 and 200cH reduced the absorbance of methylene violet (p=0.01). Repetitions and analysis of supernatants are expected to be performed in the next steps of the study. Future perspectives: Establish a pattern of reactivity of the studied medicines with different dyes and the putative relation with the corresponding supernatants, as an attempt to obtain a “physicochemical signature” for each kind of infection and/or treatment.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"51 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131107569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1190
S. Cartwright
Background: There is a pressing need to develop methods and approaches that will identify the fundamental nature of homeopathic potencies. Aims: To bring together recent basic research on potencies, especially that using solvatochromic dyes, and to supplement these results with reliable observations made by Hahnemann and his contemporaries from the very beginnings of homeopathy, together with a detailed examination of the process of trituration and succussion coupled to dilution, in order to significantly limit the number of possible explanations as to the identity of potencies. Methodology: A mixture of lab based and literature studies such that as far as possible all verified and substantiated observations about homeopathic potencies have been examined. Results and Discussion: An understanding of the fundamental nature of homeopathic potencies that includes all known and accepted observations (in vitro, in vivo and clinical) is not realistic without embracing hypotheses involving the emergent properties of complex systems and in particular, vitalistic concepts. Using a vitalistic model it is possible to explain a wide range of seemingly unrelated phenomena - such as the polarising effect of potencies on solvatochromic dyes, the ability to use a range of materials such as water, lactose and cellulose as carriers of potencies, the administering of potencies by olfaction, the antidoting effect of camphor on potency action, the non-linear dependence of potency strength on volume as well as succussion level, the oscillatory behaviour of potencies and experimenter/observer/practitioner effects. Conclusion: A hypothesis in which homeopathic potencies can be seen as self-actuating and autonomous plasma generated by trituration and/or succussion and carried according to Langmuir adsorption models fits the known observations about potencies.
{"title":"Reflections on the fundamental nature of homeopathic potencies: Plasma, Langmuir Adsorption and Vitalism","authors":"S. Cartwright","doi":"10.51910/ijhdr.v21i1.1190","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1190","url":null,"abstract":"Background: There is a pressing need to develop methods and approaches that will identify the fundamental nature of homeopathic potencies. Aims: To bring together recent basic research on potencies, especially that using solvatochromic dyes, and to supplement these results with reliable observations made by Hahnemann and his contemporaries from the very beginnings of homeopathy, together with a detailed examination of the process of trituration and succussion coupled to dilution, in order to significantly limit the number of possible explanations as to the identity of potencies. Methodology: A mixture of lab based and literature studies such that as far as possible all verified and substantiated observations about homeopathic potencies have been examined. Results and Discussion: An understanding of the fundamental nature of homeopathic potencies that includes all known and accepted observations (in vitro, in vivo and clinical) is not realistic without embracing hypotheses involving the emergent properties of complex systems and in particular, vitalistic concepts. Using a vitalistic model it is possible to explain a wide range of seemingly unrelated phenomena - such as the polarising effect of potencies on solvatochromic dyes, the ability to use a range of materials such as water, lactose and cellulose as carriers of potencies, the administering of potencies by olfaction, the antidoting effect of camphor on potency action, the non-linear dependence of potency strength on volume as well as succussion level, the oscillatory behaviour of potencies and experimenter/observer/practitioner effects. Conclusion: A hypothesis in which homeopathic potencies can be seen as self-actuating and autonomous plasma generated by trituration and/or succussion and carried according to Langmuir adsorption models fits the known observations about potencies.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128266622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1189
Cláudio Hansel Martins, Victor Tito Sperduto, Roberta Malher Bernardo, Thaís Ferreira do Espírito Santo Pires, Marcia Braga Antunes Varricchio
Background: Undergraduate students of UNIFASE/FMP, through the Academic League of Human Physiology and Pathophysiology (Liga Acadêmica de Fisiologia e Fisiopatologia Humana - LAFFH), participate in a supervised internship to outpatient at the Infectious and Parasitic Diseases Service of FMP/FASE, authorized by Clinical Direction (October 2018). Patients received conventional therapy in addition to complementary therapy with Homeopathy, for external use for cleaning skin lesions and modulating inflammatory responses, whose mechanisms of action are already described in the scientific literature and authorized by ANVISA. Aims: Make approach of undergraduate students to Homeopathic pathophysiology. Methodology: The supervised clinical internship of LAFFH students is in two service rooms with 6 students in each, based on prior appointment due to spontaneous demand, with medical records registered by the IPD outpatient service secretary. Outpatient clinical care addresses the pathophysiology and miasms of the symptoms and signs of neglected chronic parasitic diseases prevalent in our country, identified by anamnesis and physical examination. They are correlated to biological activities in plant chemistry classes, besides official homeopathic pharmacopoeia. So, it was adopted the external use of HUD Ledum palustre 6ch for the cleansing of skin lesions with torpid evolution at IPD. Results and discussion: Two patients with edema and varicose ulcers were treated with the ointment offered. Edema extension which was +++/4+ to both patients diminished to +/4+ in 30 days of interval. Indeed, ulcers remained at the same shape and size, but their bases were cleaned, without purulence. Novel clinical sessions did not occur because of social distancing needed. Conclusion: It has been developed a positive perception among patients and students about the action of homeopathic remedies. The practical teaching activity initiated encourages students in clinical reasoning about complementary therapeutic association with Homeopathy for patients whose evolution is below the expected prognosis with conventional therapy. Project of clinical observation will be elaborated to research ethics committee.
背景:UNIFASE/FMP的本科生，通过人体生理学和病理生理学学术联盟(Liga Acadêmica de Fisiologia e Fisiopatologia Humana - LAFFH)，参加由临床指导授权的FMP/FASE传染病和寄生虫病服务部的监督门诊实习(2018年10月)。患者在接受顺势疗法补充治疗的同时，接受常规治疗，用于外用清洁皮肤病变和调节炎症反应，其作用机制已在科学文献中描述并获得ANVISA授权。目的:探讨顺势疗法病理生理学在大学生中的应用。方法:LAFFH学生在两个服务室进行监督临床实习，每个服务室6名学生，根据自发需求事先预约，病历由IPD门诊秘书登记。门诊临床护理解决了在我国流行的被忽视的慢性寄生虫病的病理生理学和症状和体征，通过记忆和体格检查确定。除了官方顺势疗法药典外，它们还与植物化学课上的生物活性有关。因此，采用HUD Ledum palustre 6ch外用，用于IPD时演变缓慢的皮肤病变的清洁。结果和讨论:2例水肿和静脉曲张溃疡患者用所提供的软膏治疗。两例患者的水肿延长均为+++/4+，间隔30天减少至+/4+。事实上，溃疡的形状和大小保持不变，但它们的底部被清洁了，没有脓。由于需要保持社会距离，没有出现新的临床会议。结论:患者和学生对顺势疗法的作用有了积极的认识。开展实践教学活动，鼓励学生对发展情况低于常规治疗预期预后的患者进行顺势疗法辅助治疗关联的临床推理。临床观察项目将向研究伦理委员会阐述。
{"title":"Practical teaching of pathophysiology to LAFFH at the IPD outpatient clinic of FMP/FASE: Historical Report","authors":"Cláudio Hansel Martins, Victor Tito Sperduto, Roberta Malher Bernardo, Thaís Ferreira do Espírito Santo Pires, Marcia Braga Antunes Varricchio","doi":"10.51910/ijhdr.v21i1.1189","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1189","url":null,"abstract":"Background: Undergraduate students of UNIFASE/FMP, through the Academic League of Human Physiology and Pathophysiology (Liga Acadêmica de Fisiologia e Fisiopatologia Humana - LAFFH), participate in a supervised internship to outpatient at the Infectious and Parasitic Diseases Service of FMP/FASE, authorized by Clinical Direction (October 2018). Patients received conventional therapy in addition to complementary therapy with Homeopathy, for external use for cleaning skin lesions and modulating inflammatory responses, whose mechanisms of action are already described in the scientific literature and authorized by ANVISA. Aims: Make approach of undergraduate students to Homeopathic pathophysiology. Methodology: The supervised clinical internship of LAFFH students is in two service rooms with 6 students in each, based on prior appointment due to spontaneous demand, with medical records registered by the IPD outpatient service secretary. Outpatient clinical care addresses the pathophysiology and miasms of the symptoms and signs of neglected chronic parasitic diseases prevalent in our country, identified by anamnesis and physical examination. They are correlated to biological activities in plant chemistry classes, besides official homeopathic pharmacopoeia. So, it was adopted the external use of HUD Ledum palustre 6ch for the cleansing of skin lesions with torpid evolution at IPD. Results and discussion: Two patients with edema and varicose ulcers were treated with the ointment offered. Edema extension which was +++/4+ to both patients diminished to +/4+ in 30 days of interval. Indeed, ulcers remained at the same shape and size, but their bases were cleaned, without purulence. Novel clinical sessions did not occur because of social distancing needed. Conclusion: It has been developed a positive perception among patients and students about the action of homeopathic remedies. The practical teaching activity initiated encourages students in clinical reasoning about complementary therapeutic association with Homeopathy for patients whose evolution is below the expected prognosis with conventional therapy. Project of clinical observation will be elaborated to research ethics committee.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121929415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1186
C. Coelho, H. Gallo, Francinne Brait Narita
Background: Uric acid is the main form of excretion of nitrogenous components in birds. The assessment verification samples, control, check of uric acid concentrations is widely used to detect kidney disease. High concentrations (up to five times) of uric acid in plasma can lead to the precipitation of this acid in the form of crystals, which accumulate in the tissues, mainly in the synovial joints and the viscera surface. Aims: report the evolution of homeopathic treatment in increased uric acid in Magellanic penguins (Spheniscus magellanicus) from Santo André Municipal Zoo. Methodology: 12 animals showed an increase in uric acid (reference values 0,3-16,1mg/dL) and received homeopathic treatment with Lycopodium clavatum 6cH. The protocol established was the administration of two globules of Lycopodium clavatum 6cH, once a day, inside fishes offered in the animal's beak. Treatment started on 25/09/2019. On 30/10/2019 was collected a new blood sample, those who were not discharged were kept on treatment and new collections were made on 27/11/2019 and 17/12/2019. Results: 8 animals had an average uric acid of 20.45mg /dl and responded to the 35-days treatment, normalizing the biochemical values with an average of 12.24mg /dl. Two animals with 18.27 mg /dl were discharged in 62 days with 11.70 mg /dl and another two penguins with 19.85 mg /dl were discharged in 82 days later with 9.57 mg /dl of uric acid. Conclusion: Homeopathy proved to be successful in the treatment of increased uric acid in Magellanic penguins, easy to administer, affordable and without side effects.
{"title":"Lycopodium clavatum as a homeopathic treatment in Magellanic penguins (Sphenicus magellanicus)","authors":"C. Coelho, H. Gallo, Francinne Brait Narita","doi":"10.51910/ijhdr.v21i1.1186","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1186","url":null,"abstract":"Background: Uric acid is the main form of excretion of nitrogenous components in birds. The assessment verification samples, control, check of uric acid concentrations is widely used to detect kidney disease. High concentrations (up to five times) of uric acid in plasma can lead to the precipitation of this acid in the form of crystals, which accumulate in the tissues, mainly in the synovial joints and the viscera surface. Aims: report the evolution of homeopathic treatment in increased uric acid in Magellanic penguins (Spheniscus magellanicus) from Santo André Municipal Zoo. Methodology: 12 animals showed an increase in uric acid (reference values 0,3-16,1mg/dL) and received homeopathic treatment with Lycopodium clavatum 6cH. The protocol established was the administration of two globules of Lycopodium clavatum 6cH, once a day, inside fishes offered in the animal's beak. Treatment started on 25/09/2019. On 30/10/2019 was collected a new blood sample, those who were not discharged were kept on treatment and new collections were made on 27/11/2019 and 17/12/2019. Results: 8 animals had an average uric acid of 20.45mg /dl and responded to the 35-days treatment, normalizing the biochemical values with an average of 12.24mg /dl. Two animals with 18.27 mg /dl were discharged in 62 days with 11.70 mg /dl and another two penguins with 19.85 mg /dl were discharged in 82 days later with 9.57 mg /dl of uric acid. Conclusion: Homeopathy proved to be successful in the treatment of increased uric acid in Magellanic penguins, easy to administer, affordable and without side effects.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124569725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1202
C. Holandino, Daniela Wendhausen Beier, Míriam Fragoso, Cintia Sias Coube Bastos, Vânia Emerich Bucco Campos, A. P. Oliveira, Júlia Neves Santos, Lucas Henrique Silva, Ana Paula Canedo Valente
Background: Homeopathic ultra-diluted solutions surpass the threshold of matter dispersion and, consequently, their chemical constitution is similar to inert solvent. Nevertheless, randomized clinical trials have shown that the clinical effects of these homeopathic medicines are superior to placebo1. Nuclear magnetic resonance (NMR) is one of the most promising techniques to detect physicochemical alterations induced by homeopathic procedures2,3. Aims: To investigate T2 NMR relaxation times of Zincum metallicum and lactose dynamized samples. Methodology: Zincum metallicum samples were ground until 6dH using lactose as an excipient. Subsequently, these samples were dynamized with ultrapure water to produce 8dH, 9dH, 10dH, and 11dH. Lactose dynamized samples (6dH-11dH) were used as control. Aliquots of 540µl of each sample were diluted with 60µl of deuterated water (D2O) in 5mm tubes. The analyses were carried out in Bruker Ascend TM 500MHZ spectrometer at 288 K. Results and discussion: The Zincum metallicum and lactose T2 relaxation times were very similar, except for Zincum metallicum 8dH, which presented a value of 1.226 in comparison to 1.036 of lactose 8dH. The following T2 values were registered: 1.287 - 9dH; 1.413 - 10dH; 1.467 - 11dH, and 1.303 - 9dH; 1.400 – 10dH; 1.350 -11dH, for Zincum metallicum and lactose, respectively. The differences detected in 8dH samples are probably due to the presence of lactose in the first dilution step, in which 1 part of the 6dH triturated mixture was diluted in 9 parts of water, to prepare 7dH. Following this homeopathic procedure, 8dH solutions remain around 1% of lactose which could be influenced by the T2 values registered. Conclusion: These preliminary results showed the possibility to apply the NMR technique to evaluate the influence of dynamization in the relaxation parameters. Further studies should be carried out with other potencies and/or other homeopathic substances, in addition to the evaluation of T1 and the T1/T2 parameters, as previously described by other groups.
{"title":"NMR analysis: potential and challenges as a homeopathic basic research tool","authors":"C. Holandino, Daniela Wendhausen Beier, Míriam Fragoso, Cintia Sias Coube Bastos, Vânia Emerich Bucco Campos, A. P. Oliveira, Júlia Neves Santos, Lucas Henrique Silva, Ana Paula Canedo Valente","doi":"10.51910/ijhdr.v21i1.1202","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1202","url":null,"abstract":"Background: Homeopathic ultra-diluted solutions surpass the threshold of matter dispersion and, consequently, their chemical constitution is similar to inert solvent. Nevertheless, randomized clinical trials have shown that the clinical effects of these homeopathic medicines are superior to placebo1. Nuclear magnetic resonance (NMR) is one of the most promising techniques to detect physicochemical alterations induced by homeopathic procedures2,3. Aims: To investigate T2 NMR relaxation times of Zincum metallicum and lactose dynamized samples. Methodology: Zincum metallicum samples were ground until 6dH using lactose as an excipient. Subsequently, these samples were dynamized with ultrapure water to produce 8dH, 9dH, 10dH, and 11dH. Lactose dynamized samples (6dH-11dH) were used as control. Aliquots of 540µl of each sample were diluted with 60µl of deuterated water (D2O) in 5mm tubes. The analyses were carried out in Bruker Ascend TM 500MHZ spectrometer at 288 K. Results and discussion: The Zincum metallicum and lactose T2 relaxation times were very similar, except for Zincum metallicum 8dH, which presented a value of 1.226 in comparison to 1.036 of lactose 8dH. The following T2 values were registered: 1.287 - 9dH; 1.413 - 10dH; 1.467 - 11dH, and 1.303 - 9dH; 1.400 – 10dH; 1.350 -11dH, for Zincum metallicum and lactose, respectively. The differences detected in 8dH samples are probably due to the presence of lactose in the first dilution step, in which 1 part of the 6dH triturated mixture was diluted in 9 parts of water, to prepare 7dH. Following this homeopathic procedure, 8dH solutions remain around 1% of lactose which could be influenced by the T2 values registered. Conclusion: These preliminary results showed the possibility to apply the NMR technique to evaluate the influence of dynamization in the relaxation parameters. Further studies should be carried out with other potencies and/or other homeopathic substances, in addition to the evaluation of T1 and the T1/T2 parameters, as previously described by other groups.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125377029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1169
Mônica Filomena de Assis Souza, Kácio Gomes Macedo, David Junior de Oliveira Pereira, Graciela Andreia Flis Machado, L. V. S. Barbosa, Larissa Wolff Gonçalves, ², Pedro Henrique de Souza Cucco, M. Cucco, K. B. Brum
Background: The use of pesticides is damaging to the health of farmers and consumers as their use is associated with numerous diseases. Homeopathy is one of the techniques for organic agriculture currently authorized by the MAPA. Aims: To assess the effect of homeopathic compounds on corncob quality in pesticide-free cultures. Methodology: The experiment was performed in, MS-Brazil. Two culture fields were used, each 30m2in size. The homeopathic group treatment consisted of 1ml of30% hydroalcoholic solution with homeopathic ingredients. This solution was diluted into 30ml of water and then sprayed onto 100 seeds. The control group was submitted to the same protocol excluding the homeopathic ingredients. The homeopathic ingredients used were Sulph9C, Mag-c 12C, and Calc-p9C, prescribed according to Materia Medica instructions. The evaluation was performed at harvest by comparing the average weight of 1000 kernels per group, obtained from a weighing of 3000 kernels per group. In addition, a number of corncobs from both groups were qualitatively evaluated as “good” or “bad.” Statistics analysis used the T-test to compare the kernel weight averages and Fisher’s exact test to analyze the corncob quality frequency. Both groups were naturally infected by a virus from a neighboring sugarcane field, causing qualitative and quantitative losses on both groups. Results and discussion: The average weight of 1000 kernels from the treated group (329 g) was not significantly greater (p=0.06) than the average weight of 1000 kernels from the non-treated group (308 g). It was observed that 71out of 158 (44.9%) corncobs from the homeopathic treatment group were classified as “good” while zero out of 50 (0%) corncobs from the non-treated group were classified as “good”, resulting in a significant difference (p<0.01). Conclusion: Homeopathic compounds can be used as a feasible treatment for health and productivity in the corn crop.
{"title":"Effect of homeopathy on quality of corncobs naturally exposed to sugarcane virus","authors":"Mônica Filomena de Assis Souza, Kácio Gomes Macedo, David Junior de Oliveira Pereira, Graciela Andreia Flis Machado, L. V. S. Barbosa, Larissa Wolff Gonçalves, ², Pedro Henrique de Souza Cucco, M. Cucco, K. B. Brum","doi":"10.51910/ijhdr.v21i1.1169","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1169","url":null,"abstract":"Background: The use of pesticides is damaging to the health of farmers and consumers as their use is associated with numerous diseases. Homeopathy is one of the techniques for organic agriculture currently authorized by the MAPA. Aims: To assess the effect of homeopathic compounds on corncob quality in pesticide-free cultures. Methodology: The experiment was performed in, MS-Brazil. Two culture fields were used, each 30m2in size. The homeopathic group treatment consisted of 1ml of30% hydroalcoholic solution with homeopathic ingredients. This solution was diluted into 30ml of water and then sprayed onto 100 seeds. The control group was submitted to the same protocol excluding the homeopathic ingredients. The homeopathic ingredients used were Sulph9C, Mag-c 12C, and Calc-p9C, prescribed according to Materia Medica instructions. The evaluation was performed at harvest by comparing the average weight of 1000 kernels per group, obtained from a weighing of 3000 kernels per group. In addition, a number of corncobs from both groups were qualitatively evaluated as “good” or “bad.” Statistics analysis used the T-test to compare the kernel weight averages and Fisher’s exact test to analyze the corncob quality frequency. Both groups were naturally infected by a virus from a neighboring sugarcane field, causing qualitative and quantitative losses on both groups. Results and discussion: The average weight of 1000 kernels from the treated group (329 g) was not significantly greater (p=0.06) than the average weight of 1000 kernels from the non-treated group (308 g). It was observed that 71out of 158 (44.9%) corncobs from the homeopathic treatment group were classified as “good” while zero out of 50 (0%) corncobs from the non-treated group were classified as “good”, resulting in a significant difference (p<0.01). Conclusion: Homeopathic compounds can be used as a feasible treatment for health and productivity in the corn crop.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114501809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1178
A. S. Marquez, T. Passeti, S. Diniz, Everton Tadeu Prado
Introduction: Homeopathy is a therapy that uses medications prepared with infinitesimal and dynamized dilutions. Current studies demonstrate in vitro activity of homeopathy on gram-positive bacteria such as Staphylococcus aureus and Streptococcus pyogenes. Among bacterial infections, urinary tract infection (UTI) is frequent, leads to later consequences and the main causal agent is Escherichia coli (E. coli). In other publications, it has been reported inactivity of homeopathy on E. coli cultures. Due to the divergence in the literature, the objective of this study was to evaluate gram-negative bacteria growth under homeopathy treatment. Methods: The medicines Atropa belladona, Cantharis, Staphysagria, and Colibacillinum were tested at 6CH, 12CH and 30CH in E. coli ATCC 25922 and EPEC (Enteropathogenic Escherichia coli) ATCC 43887. Two hundred and fifty microliters of the medicines in alcohol 30% were incubated at 37ºC with 3 mL of Müller Hinton broth (MH), 10 μL of cultures at 0.5 Macfarland and subsequent dilution at 1/10. Bacterial growth was evaluated in a spectrophotometer at 600nm, in the periods of 6, 12, and 20 hours of incubation. Results and Discussion: The results showed no inhibition of bacterial growth under the studied conditions. These data corroborate with studies already published that indicate the absence of action of homeopathy on E. coli cultures. Considering other studies, it can be suggested that homeopathic medicines have direct activity on the growth of Gram-positive and not Gram-negative bacteria. Evaluating the two bacterial groups, it is possible to assume that the difference in homeopathy activity could be linked to differences in the bacterial wall structure. This hypothesis should be evaluated by other tests with the same bacterial strains. Conclusion: The homeopathic medicines tested have no direct activity on Gram-negative bacteria cultures.
{"title":"Homeopathic medicine activity on the growth of Gram-negative bacteria","authors":"A. S. Marquez, T. Passeti, S. Diniz, Everton Tadeu Prado","doi":"10.51910/ijhdr.v21i1.1178","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1178","url":null,"abstract":"Introduction: Homeopathy is a therapy that uses medications prepared with infinitesimal and dynamized dilutions. Current studies demonstrate in vitro activity of homeopathy on gram-positive bacteria such as Staphylococcus aureus and Streptococcus pyogenes. Among bacterial infections, urinary tract infection (UTI) is frequent, leads to later consequences and the main causal agent is Escherichia coli (E. coli). In other publications, it has been reported inactivity of homeopathy on E. coli cultures. Due to the divergence in the literature, the objective of this study was to evaluate gram-negative bacteria growth under homeopathy treatment. Methods: The medicines Atropa belladona, Cantharis, Staphysagria, and Colibacillinum were tested at 6CH, 12CH and 30CH in E. coli ATCC 25922 and EPEC (Enteropathogenic Escherichia coli) ATCC 43887. Two hundred and fifty microliters of the medicines in alcohol 30% were incubated at 37ºC with 3 mL of Müller Hinton broth (MH), 10 μL of cultures at 0.5 Macfarland and subsequent dilution at 1/10. Bacterial growth was evaluated in a spectrophotometer at 600nm, in the periods of 6, 12, and 20 hours of incubation. Results and Discussion: The results showed no inhibition of bacterial growth under the studied conditions. These data corroborate with studies already published that indicate the absence of action of homeopathy on E. coli cultures. Considering other studies, it can be suggested that homeopathic medicines have direct activity on the growth of Gram-positive and not Gram-negative bacteria. Evaluating the two bacterial groups, it is possible to assume that the difference in homeopathy activity could be linked to differences in the bacterial wall structure. This hypothesis should be evaluated by other tests with the same bacterial strains. Conclusion: The homeopathic medicines tested have no direct activity on Gram-negative bacteria cultures.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133875352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1192
M. Mendes, P. Oliveira, Maria Solange Gosik, R. Lino, R. Kalile, D. Barbas, Romeu Carillo Jr
Background: The literature associates oxidative stress with the production of free radicals, which lead to neurodegeneration. They present innumerable hypotheses, among which are abnormalities in the functioning of the hypothalamic-pituitary-adrenal axis, neurotoxic effects and neuronal oxidative damage. Clinical observation has shown that in neurodegenerative diseases such as Multiple Sclerosis (MS) and Amyotrophic Lateral Sclerosis (ALS) there is a report of prolonged or violent emotional stress preceding the symptoms. Aims: Using the Carillo Complex Systems Model, present some possibilities on how stress can contribute to neurodegeneration. Methodology: Nine cases of ALS and six cases of MS were evaluated, pathologies already classified as belonging to syphilinism. Literature review on stress and neurotoxicity carried out. Results and discussion: Syphilinism is an instability with a predominantly intrisic origin to the system with a chronic caracter. This diathesis characterized by a dissipative deficiency, predominantly hepatic, to the processing of certain elements or potentially toxic substances with exogenous origin or endogenous Such non-processed substances are unstable factors in the system, with greater affinity for certain tissues, like the nervous system. Among the toxins, we find alcohol, esters, formaldehyde, aloe, ketones, aldehydes, etc. The final hepatic metabolism of cortisol results in cortic acids and cortol, which use the same enzymatic system as alcohol, and can be considered syphilinic toxins. Ethanol can act directly at the circadian rhythm, disrupting it and generating stressful substances such as cortisol, regardless of an external event, increasing the toxin level. The inflammatory process generated by the production of free radicals and metabolic abnormalities, including the reduction of neuropeptide Y that modulates inflammatory activity in the nervous system, leads to changes that can result in neurodegeneration. Conclusion: Inflammation caused by toxins from
{"title":"Stress - another possible mechanism of neurotoxicity in neurodegenerative diseases","authors":"M. Mendes, P. Oliveira, Maria Solange Gosik, R. Lino, R. Kalile, D. Barbas, Romeu Carillo Jr","doi":"10.51910/ijhdr.v21i1.1192","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1192","url":null,"abstract":"Background: The literature associates oxidative stress with the production of free radicals, which lead to neurodegeneration. They present innumerable hypotheses, among which are abnormalities in the functioning of the hypothalamic-pituitary-adrenal axis, neurotoxic effects and neuronal oxidative damage. Clinical observation has shown that in neurodegenerative diseases such as Multiple Sclerosis (MS) and Amyotrophic Lateral Sclerosis (ALS) there is a report of prolonged or violent emotional stress preceding the symptoms. Aims: Using the Carillo Complex Systems Model, present some possibilities on how stress can contribute to neurodegeneration. Methodology: Nine cases of ALS and six cases of MS were evaluated, pathologies already classified as belonging to syphilinism. Literature review on stress and neurotoxicity carried out. Results and discussion: Syphilinism is an instability with a predominantly intrisic origin to the system with a chronic caracter. This diathesis characterized by a dissipative deficiency, predominantly hepatic, to the processing of certain elements or potentially toxic substances with exogenous origin or endogenous Such non-processed substances are unstable factors in the system, with greater affinity for certain tissues, like the nervous system. Among the toxins, we find alcohol, esters, formaldehyde, aloe, ketones, aldehydes, etc. The final hepatic metabolism of cortisol results in cortic acids and cortol, which use the same enzymatic system as alcohol, and can be considered syphilinic toxins. Ethanol can act directly at the circadian rhythm, disrupting it and generating stressful substances such as cortisol, regardless of an external event, increasing the toxin level. The inflammatory process generated by the production of free radicals and metabolic abnormalities, including the reduction of neuropeptide Y that modulates inflammatory activity in the nervous system, leads to changes that can result in neurodegeneration. Conclusion: Inflammation caused by toxins from","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122864778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1173
R. Nardy, A. M. Nunes, L. S. Barroso, Cristina Garani Schneider Guimarães
Introduction: Arnica montana 6CH is a medicine indicated for inflammatory and painful conditions, especially in muscle structures. The RDC / TMD Axis II questionnaire is a validated tool for the diagnosis of Temporomandibular Muscle Dysfunction. Objective: This multicenter, randomized and controlled study, approved by Human or Animal Research Ethics Committee UniFOA - CAAE: 48680015.3.0000.5237 aimed to verify the clinical performance of Arnica montana 6CH in contractures and muscle pain triggered by isometric stress. Materials and Methods: 70 patients underwent prolonged dental treatment sessions, and they were selected after clinical examination and positive responses to the RDC / TMD questionnaire to confirm signs and symptoms of Temporomandibular Muscle Dysfunction at the first consultation. Randomly, Arnica montana 6CH was prescribed for 35 patients called group I, and placebo for 35 patients called group II, at a dose of 5 globules, 03 times a day, for 1 week. In the second endodontic consultation, after 15 days, a new clinical examination was performed at the beginning and end of the prolonged dental consultation with crossing of data from the RDC questionnaire, to monitor the prevalence of TMD muscle signs and symptoms. The data were tabulated and analyzed. Statistical analysis: The test t de Student was used for paired samples, significant at the level ≤ 0.05%. Results: Anamnetic data from the RDC questionnaire, 86% of the individuals in the GI had lower pain and muscle contracture rates in the second consultation, compared with 22% in the GII. Conclusion: The drug Arnica montana 6CH proved to be effective in preventing muscle changes and clinical symptoms resulting from isometric efforts with a statistically significant difference (p ≤ 0.05).
{"title":"Evaluation of the effectiveness of arnica montana 6ch in the control of postoperative myalgia by isometric effort in dentistry","authors":"R. Nardy, A. M. Nunes, L. S. Barroso, Cristina Garani Schneider Guimarães","doi":"10.51910/ijhdr.v21i1.1173","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1173","url":null,"abstract":"Introduction: Arnica montana 6CH is a medicine indicated for inflammatory and painful conditions, especially in muscle structures. The RDC / TMD Axis II questionnaire is a validated tool for the diagnosis of Temporomandibular Muscle Dysfunction. Objective: This multicenter, randomized and controlled study, approved by Human or Animal Research Ethics Committee UniFOA - CAAE: 48680015.3.0000.5237 aimed to verify the clinical performance of Arnica montana 6CH in contractures and muscle pain triggered by isometric stress. Materials and Methods: 70 patients underwent prolonged dental treatment sessions, and they were selected after clinical examination and positive responses to the RDC / TMD questionnaire to confirm signs and symptoms of Temporomandibular Muscle Dysfunction at the first consultation. Randomly, Arnica montana 6CH was prescribed for 35 patients called group I, and placebo for 35 patients called group II, at a dose of 5 globules, 03 times a day, for 1 week. In the second endodontic consultation, after 15 days, a new clinical examination was performed at the beginning and end of the prolonged dental consultation with crossing of data from the RDC questionnaire, to monitor the prevalence of TMD muscle signs and symptoms. The data were tabulated and analyzed. Statistical analysis: The test t de Student was used for paired samples, significant at the level ≤ 0.05%. Results: Anamnetic data from the RDC questionnaire, 86% of the individuals in the GI had lower pain and muscle contracture rates in the second consultation, compared with 22% in the GII. Conclusion: The drug Arnica montana 6CH proved to be effective in preventing muscle changes and clinical symptoms resulting from isometric efforts with a statistically significant difference (p ≤ 0.05).","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"219 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115983093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-06DOI: 10.51910/ijhdr.v21i1.1187
Adalberto von Ancken, F. Eizayaga, L. Bonamin
Background: Under LPS-stimulus, platelets can activate macrophages by a cell-to-cell contact or through cytokine degranulation. Rebound effects of anti-thrombotic agents, such as prostanoids and COX inhibitors can lead to thrombosis, infarct, and stroke. Aspirin has been prescribed for decades due to its powerful antiplatelet action, but it is also related to paradoxical effects such as withdrawal syndrome peaks, resistance, and thrombogenesis. Ultra-diluted aspirin can also produce the same effect in one hour, regardless of Cox-2, by still unknown pathways. Antithrombotic effects of aspirin are also reversed by its high dilutions. Aims: This study aims to characterize the effects of aspirin 15cH on macrophages challenged with LPS, a Cox-2 activator. Methodology: RAW 264.7 macrophages were sown in 24 wells plates using R10 medium, boosted with 1μg/ml LPS, and treated with aspirin 15 cH and controls. The activity was evaluated after 24 hours. Supernatants were evaluated for cytokines, nitric oxide, and dielectric oscillations, through solvatochromic dyes (Cartwright’s method). Results and discussion: macrophage spreading was increased by aspirin 15 cH, an LPS-like effect. Paradoxically, a significant reduction of this effect was noted when both, LPS and aspirin 15 cH, were added. Succussed water reversed the effect of LPS, leading to TNF (p<0.05) production close to baseline levels. Also, the single treatment with succussed water inhibited IL-10 production (p<0.05), but aspirin 200 µg/mL (positive control) highly increased it (p<0.0001), showing the validity of the model. Nitric oxide production was strengthened by LPS presence (p<0.0001), as expected, but partially downregulated after treatment with aspirin 200 µg/mL, water and succussed water. A pilot study with solvatochromic dyes showed no significant difference among treatments. Conclusion: The main data suggest that aspirin 15 cH increases macrophage activity but presents a paradoxal effect when mixed with LPS. On the other hand, succussed water itself has modulatory effects on macrophages.
{"title":"Macrophage activity regulation by high dilution of aspirin and its possible mechanism","authors":"Adalberto von Ancken, F. Eizayaga, L. Bonamin","doi":"10.51910/ijhdr.v21i1.1187","DOIUrl":"https://doi.org/10.51910/ijhdr.v21i1.1187","url":null,"abstract":"Background: Under LPS-stimulus, platelets can activate macrophages by a cell-to-cell contact or through cytokine degranulation. Rebound effects of anti-thrombotic agents, such as prostanoids and COX inhibitors can lead to thrombosis, infarct, and stroke. Aspirin has been prescribed for decades due to its powerful antiplatelet action, but it is also related to paradoxical effects such as withdrawal syndrome peaks, resistance, and thrombogenesis. Ultra-diluted aspirin can also produce the same effect in one hour, regardless of Cox-2, by still unknown pathways. Antithrombotic effects of aspirin are also reversed by its high dilutions. Aims: This study aims to characterize the effects of aspirin 15cH on macrophages challenged with LPS, a Cox-2 activator. Methodology: RAW 264.7 macrophages were sown in 24 wells plates using R10 medium, boosted with 1μg/ml LPS, and treated with aspirin 15 cH and controls. The activity was evaluated after 24 hours. Supernatants were evaluated for cytokines, nitric oxide, and dielectric oscillations, through solvatochromic dyes (Cartwright’s method). Results and discussion: macrophage spreading was increased by aspirin 15 cH, an LPS-like effect. Paradoxically, a significant reduction of this effect was noted when both, LPS and aspirin 15 cH, were added. Succussed water reversed the effect of LPS, leading to TNF (p<0.05) production close to baseline levels. Also, the single treatment with succussed water inhibited IL-10 production (p<0.05), but aspirin 200 µg/mL (positive control) highly increased it (p<0.0001), showing the validity of the model. Nitric oxide production was strengthened by LPS presence (p<0.0001), as expected, but partially downregulated after treatment with aspirin 200 µg/mL, water and succussed water. A pilot study with solvatochromic dyes showed no significant difference among treatments. Conclusion: The main data suggest that aspirin 15 cH increases macrophage activity but presents a paradoxal effect when mixed with LPS. On the other hand, succussed water itself has modulatory effects on macrophages.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126443138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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