Pub Date : 2023-12-20DOI: 10.51910/ijhdr.v22i2.1352
Alexandre Prospero
The objective of this work is to argue about the possibility of remote work and the utilization of communication technology, enabling Tele-veterinary homeopathy to emerge as a specialized field for prescribing medical materials, primarily in cases of behavioral changes. However, the institutions regulating veterinary telemedicine and veterinary medical activities assert that face-to-face care remains the gold standard for practicing veterinary medical procedures. Teleconsultation is only permissible in cases where there exists a prior Veterinary-Animal-Responsible Relationship (RPVAR) that has been established through face-to-face interactions and duly registered.The administration of medicines is considered safe, as they are non-toxic and devoid of side effects due to their ultra-diluted and dynamized formulation, exclusive to this scientific modality. Furthermore, when recommended by a professional specialist and facilitated through telemedicine resources, the continuity of patient care and monitoring will face no hindrances. It is important to emphasize that even though the prescribed medications for veterinary patients are safe, they should be vigilantly monitored by a specialist. This monitoring involves assessing the expected symptoms and effects associated with the prescription, allowing the specialist to intervene and decide whether to modify or continue with the chosen medication.
{"title":"Telemedicine and homeopathy united for wellness and health in Brazilian veterinary.","authors":"Alexandre Prospero","doi":"10.51910/ijhdr.v22i2.1352","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1352","url":null,"abstract":"The objective of this work is to argue about the possibility of remote work and the utilization of communication technology, enabling Tele-veterinary homeopathy to emerge as a specialized field for prescribing medical materials, primarily in cases of behavioral changes. However, the institutions regulating veterinary telemedicine and veterinary medical activities assert that face-to-face care remains the gold standard for practicing veterinary medical procedures. Teleconsultation is only permissible in cases where there exists a prior Veterinary-Animal-Responsible Relationship (RPVAR) that has been established through face-to-face interactions and duly registered.The administration of medicines is considered safe, as they are non-toxic and devoid of side effects due to their ultra-diluted and dynamized formulation, exclusive to this scientific modality. Furthermore, when recommended by a professional specialist and facilitated through telemedicine resources, the continuity of patient care and monitoring will face no hindrances. It is important to emphasize that even though the prescribed medications for veterinary patients are safe, they should be vigilantly monitored by a specialist. This monitoring involves assessing the expected symptoms and effects associated with the prescription, allowing the specialist to intervene and decide whether to modify or continue with the chosen medication.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.51910/ijhdr.v22i2.1325
Steven J. Cartwright
Using a high specification potentiser (Pinkus Potentiser) capable of consistently and reliably producing homeopathic potencies to rigorous standards, unsuccussed control water, Water 10M and Arsenicum 10M have been compared with respect to their effect on the solvatochromic dye phenol blue. Water 10M was found to have no statistically significant effect compared with unsuccussed water whilst Arsenicum 10M produced a statistically significant effect different from both unsuccussed water and Water 10M. The conclusion from this result is that bulk pure water does not potentise and is no different from unsuccussed water. This in turn indicates that what is potentised during cycles of dilution and succussion are the substances placed in water and not water itself, and that water is simply an inert carrier of the homeopathic signal. Evidence from the practice of homeopathy, in particular the storage and administering of homeopathic medicines, supports this conclusion.
{"title":"Evidence from the use of a solvatochromic detection system indicates that water is an inert carrier of the homeopathic signal","authors":"Steven J. Cartwright","doi":"10.51910/ijhdr.v22i2.1325","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1325","url":null,"abstract":"Using a high specification potentiser (Pinkus Potentiser) capable of consistently and reliably producing homeopathic potencies to rigorous standards, unsuccussed control water, Water 10M and Arsenicum 10M have been compared with respect to their effect on the solvatochromic dye phenol blue. Water 10M was found to have no statistically significant effect compared with unsuccussed water whilst Arsenicum 10M produced a statistically significant effect different from both unsuccussed water and Water 10M. The conclusion from this result is that bulk pure water does not potentise and is no different from unsuccussed water. This in turn indicates that what is potentised during cycles of dilution and succussion are the substances placed in water and not water itself, and that water is simply an inert carrier of the homeopathic signal. Evidence from the practice of homeopathy, in particular the storage and administering of homeopathic medicines, supports this conclusion.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"212 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1365
E. C. Perez, Larissa Béja, Thiago Albuquerque Viração, Jaqueline De Carvalho Arjona, Sandra Kalil, Leoni Villano Binamin
Breast cancer is a malignant tumor frequently diagnosed in the female population that representsthe main cause of cancer death in women worldwide. Recently, the use of complementary therapiessuch as homeopathy has increased to alleviate the side effects of conventional allopathic treatments.However, the cellular and molecular effects of homeopathic medicaments have not been fullyclarified. Thus, the aim of this work was to evaluate the in vitro effects of Phytolacca decandrahomeopathic preparations on human breast adenocarcinoma cells. For this, MCF-7 breastadenocarcinoma cells were cultivated in the presence or absence of Phytolacca decandra mothertincture (PdTM) or centesimal Hahnemannian dilution (CH) of Phytolacca decandra: 30 or 200 CH.Determination of apoptosis and necrosis percentages, cytokine production and morphologicalchanges were evaluated after 24, 48 and 72 hours of culture in each of the treatments. Resultsobtained in cytotoxicity analyses showed a higher percentage of death in cells treated withhomeopathic dilutions, with similar results to that presented by cells treated with Doxorubicin orPdTM. In contrast, cells treated with homeopathic dilutions of Phytolacca decandra did not showmarked differences in the production of pro and anti-inflammatory cytokines when compared tountreated. Similarly, analyses of cell morphology did not display significant differences before andafter treatment with homeopathic dilutions, when compared to untreated cells. Already, cells treatedwith PdTM presented marked cellular alterations with observation of apoptotic bodies in the threeevaluated times. Together, results show that homeopathic dilutions of Phytolacca decandra inducedcytotoxicity of human breast adenocarcinoma cells in the absence of inflammatory process.
{"title":"Homeopathic dilutions of Phytolacca decandra induce cytotoxicity of human breast adenocarcinoma cells","authors":"E. C. Perez, Larissa Béja, Thiago Albuquerque Viração, Jaqueline De Carvalho Arjona, Sandra Kalil, Leoni Villano Binamin","doi":"10.51910/ijhdr.v22i2.1365","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1365","url":null,"abstract":"Breast cancer is a malignant tumor frequently diagnosed in the female population that representsthe main cause of cancer death in women worldwide. Recently, the use of complementary therapiessuch as homeopathy has increased to alleviate the side effects of conventional allopathic treatments.However, the cellular and molecular effects of homeopathic medicaments have not been fullyclarified. Thus, the aim of this work was to evaluate the in vitro effects of Phytolacca decandrahomeopathic preparations on human breast adenocarcinoma cells. For this, MCF-7 breastadenocarcinoma cells were cultivated in the presence or absence of Phytolacca decandra mothertincture (PdTM) or centesimal Hahnemannian dilution (CH) of Phytolacca decandra: 30 or 200 CH.Determination of apoptosis and necrosis percentages, cytokine production and morphologicalchanges were evaluated after 24, 48 and 72 hours of culture in each of the treatments. Resultsobtained in cytotoxicity analyses showed a higher percentage of death in cells treated withhomeopathic dilutions, with similar results to that presented by cells treated with Doxorubicin orPdTM. In contrast, cells treated with homeopathic dilutions of Phytolacca decandra did not showmarked differences in the production of pro and anti-inflammatory cytokines when compared tountreated. Similarly, analyses of cell morphology did not display significant differences before andafter treatment with homeopathic dilutions, when compared to untreated cells. Already, cells treatedwith PdTM presented marked cellular alterations with observation of apoptotic bodies in the threeevaluated times. Together, results show that homeopathic dilutions of Phytolacca decandra inducedcytotoxicity of human breast adenocarcinoma cells in the absence of inflammatory process.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"71 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1356
Gleyce Moreno Barbosa, Amanda Ribeiro Ricardo Brito, Paloma Barbosa da Silva Moura, Camily Da Silva Faria, Lais Cavalcanti dos Santos Velasco de Souza, Leandro Machado Rocha, Andrea Regina de Souza Baptista
Background: Sporothrix brasiliensis is a fungus that affects animals, mainly felines, and humans. The infection can present as subcutaneous or disseminated form in the organism. Rio de Janeiro, Brazil, is a state with high prevalence of cases in felines. Currently, the standard treatment is realized during a long period and using itraconazole, that causes important adverse effects. Objective: The aim of this research was to evaluate in vitro effect of Sporothrix brasiliensis biotherapic in the fungus used to prepare this medicine, also considering the association to itraconazole. Methodology: Yeast cells of S. brasiliensis (ATCC MYA 4823) were cultured in Brain Heart Infusion (BHI) during 3 to 5 days. The biotherapic 30 DH was prepared according to Brazilian Homeopathic Pharmacopoeia, using 5 x 107 yeasts/mL. Two biotherapics were prepared: using inactivated fungus by autoclave (Sb1) and viable yeasts (Sb2). Both biotherapics were evaluated individually and associated to itraconazole. The control groups were: water, diluted water, water 30 DH, and dimethylsulfoxide (vehicle to dissolve itraconazole), and itraconazole. The minimum inhibitory concentration (MIC) experiments were carried out, considering the CLSI (Clinical & Laboratory Standards Institute) guidelines. Results: The treatments using only biotherapic did not reduced the yeast viability, as observed in the controls. However, the higher concentrations of itraconazole and of this drug associated with biotherapic reduced the viability of S. brasiliensis. Then, the observed effect was attributed to itraconazole. Conclusion: As expected, it was not detected the effect of both biotherapic in the viability of microorganism itself, due to the integrality aspects of homeopathy, because the homeopathic medicine emphasizes the whole organism’s response to the symptoms presented. Then, the next step is to evaluate the biotherapic in an in vivo model, that will be a complete organism representing the host to this infection.
{"title":"In vitro evaluation of Sporothrix brasiliensis biotherapic: complete results","authors":"Gleyce Moreno Barbosa, Amanda Ribeiro Ricardo Brito, Paloma Barbosa da Silva Moura, Camily Da Silva Faria, Lais Cavalcanti dos Santos Velasco de Souza, Leandro Machado Rocha, Andrea Regina de Souza Baptista","doi":"10.51910/ijhdr.v22i2.1356","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1356","url":null,"abstract":"Background: Sporothrix brasiliensis is a fungus that affects animals, mainly felines, and humans. The infection can present as subcutaneous or disseminated form in the organism. Rio de Janeiro, Brazil, is a state with high prevalence of cases in felines. Currently, the standard treatment is realized during a long period and using itraconazole, that causes important adverse effects. Objective: The aim of this research was to evaluate in vitro effect of Sporothrix brasiliensis biotherapic in the fungus used to prepare this medicine, also considering the association to itraconazole. Methodology: Yeast cells of S. brasiliensis (ATCC MYA 4823) were cultured in Brain Heart Infusion (BHI) during 3 to 5 days. The biotherapic 30 DH was prepared according to Brazilian Homeopathic Pharmacopoeia, using 5 x 107 yeasts/mL. Two biotherapics were prepared: using inactivated fungus by autoclave (Sb1) and viable yeasts (Sb2). Both biotherapics were evaluated individually and associated to itraconazole. The control groups were: water, diluted water, water 30 DH, and dimethylsulfoxide (vehicle to dissolve itraconazole), and itraconazole. The minimum inhibitory concentration (MIC) experiments were carried out, considering the CLSI (Clinical & Laboratory Standards Institute) guidelines. Results: The treatments using only biotherapic did not reduced the yeast viability, as observed in the controls. However, the higher concentrations of itraconazole and of this drug associated with biotherapic reduced the viability of S. brasiliensis. Then, the observed effect was attributed to itraconazole. Conclusion: As expected, it was not detected the effect of both biotherapic in the viability of microorganism itself, due to the integrality aspects of homeopathy, because the homeopathic medicine emphasizes the whole organism’s response to the symptoms presented. Then, the next step is to evaluate the biotherapic in an in vivo model, that will be a complete organism representing the host to this infection.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"142 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1364
Thais C Da Silva, Carla Quaresma Holandino, S. A. Gordinho Pinto, Débora De Oliveira Mares Silvestro, Ivana Barbosa Suffredini, Priscila Inhauser Baltuille do Prado, Hellen Denise Rocha, Bárbara Hiramuki, Vitoria Yasmin Silva Laranja, Leoni Villano Bonamin, E. C. Perez
Recently, several authors have shown the benefits of homeopathy in improving the quality of lifeand survival of cancer patients. Preliminary data from the group showed that B16F10 murinemelanoma cells treated with Viscum album (Va), collected from the host tree Quercus robur duringthe Swiss summer (VaQr.s) and prepared in different homeopathic scales: 12 dH, 200 cH and 5 lmdecreased both viability and production of reactive oxygen species (ROS) and MCP-1 cytokine,when compared to B16F10 cells treated with the chemotherapy drug carboplatin. However, the invivo effects of these treatments have not been evaluated. Therefore, the aim of the present workwas to investigate the effects of different homeopathic preparations of VaQr.s in mice withmelanoma. For this, C57BL/6 mice were inoculated subcutaneously (s.c) on the left flank withB16F10 cells. After appearance of tumor nodule, mice were treated three times a week, orally,during 3 weeks with mother tincture (TM) or different potencies of VaQr.s (12 dH, 200 cH or5 lm). As controls, mice were treated with water or vehicle (10%-alcohol) or chemotherapy(carboplatin). Evaluations of the animals' general condition and tumor growth were performed onalternate days until approximately 30 days after tumor cell inoculation. The results obtainedshowed that mice treated with VaQr 12 dH or 5 lm have increased survival and body weight, aswell as a tendency to increase the quality of life of animals with melanoma, since these presentedless clinical manifestations when compared to the other treatments, mainly the carboplatin group.Together, the data presented here demonstrated for the first time, the effects of Va potencies onalleviating cancer-related fatigue in an in vivo model of murine melanoma. Furthermore, this workprovides relevant information for understanding the molecular events induced by homeopathicpreparations of Va as a support for future studies with ultradiluted Va
{"title":"Effects of homeopathic preparations of Viscum album on in vivo murine melanoma model","authors":"Thais C Da Silva, Carla Quaresma Holandino, S. A. Gordinho Pinto, Débora De Oliveira Mares Silvestro, Ivana Barbosa Suffredini, Priscila Inhauser Baltuille do Prado, Hellen Denise Rocha, Bárbara Hiramuki, Vitoria Yasmin Silva Laranja, Leoni Villano Bonamin, E. C. Perez","doi":"10.51910/ijhdr.v22i2.1364","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1364","url":null,"abstract":"Recently, several authors have shown the benefits of homeopathy in improving the quality of lifeand survival of cancer patients. Preliminary data from the group showed that B16F10 murinemelanoma cells treated with Viscum album (Va), collected from the host tree Quercus robur duringthe Swiss summer (VaQr.s) and prepared in different homeopathic scales: 12 dH, 200 cH and 5 lmdecreased both viability and production of reactive oxygen species (ROS) and MCP-1 cytokine,when compared to B16F10 cells treated with the chemotherapy drug carboplatin. However, the invivo effects of these treatments have not been evaluated. Therefore, the aim of the present workwas to investigate the effects of different homeopathic preparations of VaQr.s in mice withmelanoma. For this, C57BL/6 mice were inoculated subcutaneously (s.c) on the left flank withB16F10 cells. After appearance of tumor nodule, mice were treated three times a week, orally,during 3 weeks with mother tincture (TM) or different potencies of VaQr.s (12 dH, 200 cH or5 lm). As controls, mice were treated with water or vehicle (10%-alcohol) or chemotherapy(carboplatin). Evaluations of the animals' general condition and tumor growth were performed onalternate days until approximately 30 days after tumor cell inoculation. The results obtainedshowed that mice treated with VaQr 12 dH or 5 lm have increased survival and body weight, aswell as a tendency to increase the quality of life of animals with melanoma, since these presentedless clinical manifestations when compared to the other treatments, mainly the carboplatin group.Together, the data presented here demonstrated for the first time, the effects of Va potencies onalleviating cancer-related fatigue in an in vivo model of murine melanoma. Furthermore, this workprovides relevant information for understanding the molecular events induced by homeopathicpreparations of Va as a support for future studies with ultradiluted Va","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"27 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1367
Priscila Baltuille, Thaís Cristina Silva, E. C. Hurtado, C. Holandino, Stephan Baumgartner, L. Bonamin
Melanoma is responsible for most skin cancer deaths. It has a high metastatic potential, complex treatment, and a hopeless prognosis. Viscum album extract (Va) is a complementary treatment in cancer, with in vitro and in vivo cytotoxic effects on different tumor types. However, its ultra-diluted homeopathic preparations' effects and mechanisms on cancer cells need further study. Thus, the aim of present work was to investigate cytokine profile and ROs production of melanoma cells after treatment with ultradiluted VA collected from host tree Abies alba (VaAa) and Quercus rubur (VaQr)during Swiss summer. For this, potencies of Va in different homeopathic scales were prepared using mother tincture (MT) from VaAa and VaQr submitted to homeopathic ethanolic maceration and then a subsequent dynamization process. Then, B16F0 melanoma cells cultivated in 24-well plates were treated with MT or 12DH, 200CH or 5LM potencies of VaAa or VaQr for 24, 48 or 72 hours. After these periods, culture supernatants were collected to assess the levels of MCP-1, INF-γ, TNF-α, IL-10, IL-12p70 and IL-6 cytokines and, ROs production. Results demonstrated significant reduction of MCP-1 and ROs levels in B16F10 melanoma cells treated with Va (VaQr, preferentially) at 12DH or 5LM potencies when compared to untreated or carboplatin-treated cells. In conclusion, we demonstrated for the first time, the in vitro effects of Va potencies prepared in different homeopathic scales in relation to cytokines and ROs production in cell melanoma cells.
{"title":"Treatment with Viscum album potencies induces reduction of MCP-1 and ROs levels in murine melanoma cells.","authors":"Priscila Baltuille, Thaís Cristina Silva, E. C. Hurtado, C. Holandino, Stephan Baumgartner, L. Bonamin","doi":"10.51910/ijhdr.v22i2.1367","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1367","url":null,"abstract":"Melanoma is responsible for most skin cancer deaths. It has a high metastatic potential, complex treatment, and a hopeless prognosis. Viscum album extract (Va) is a complementary treatment in cancer, with in vitro and in vivo cytotoxic effects on different tumor types. However, its ultra-diluted homeopathic preparations' effects and mechanisms on cancer cells need further study. Thus, the aim of present work was to investigate cytokine profile and ROs production of melanoma cells after treatment with ultradiluted VA collected from host tree Abies alba (VaAa) and Quercus rubur (VaQr)during Swiss summer. For this, potencies of Va in different homeopathic scales were prepared using mother tincture (MT) from VaAa and VaQr submitted to homeopathic ethanolic maceration and then a subsequent dynamization process. Then, B16F0 melanoma cells cultivated in 24-well plates were treated with MT or 12DH, 200CH or 5LM potencies of VaAa or VaQr for 24, 48 or 72 hours. After these periods, culture supernatants were collected to assess the levels of MCP-1, INF-γ, TNF-α, IL-10, IL-12p70 and IL-6 cytokines and, ROs production. Results demonstrated significant reduction of MCP-1 and ROs levels in B16F10 melanoma cells treated with Va (VaQr, preferentially) at 12DH or 5LM potencies when compared to untreated or carboplatin-treated cells. In conclusion, we demonstrated for the first time, the in vitro effects of Va potencies prepared in different homeopathic scales in relation to cytokines and ROs production in cell melanoma cells.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"205 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1360
Akshaya Tharankini, D. L. Prabu, V. W. Vargheese
Background: Globally, 2.4 million neonates succumbed in 2019, constituting 47% of all under-5 mortalities[1]. India bore the highest neonatal mortality in the same year, attributed predominantly to infections[2]. Innate immunity, bestowed at birth, represents our primary defense against neonatal mortality. Exposure to crude forms of arsenic in zebrafish embryos has been shown to significantly down-regulate the innate immune responses[3-8]. Such studies reveal the realm of operation, susceptible cells, and mechanisms through which arsenic can engage with our system, aiding in the understanding of the Homoeopathic medicine Arsenicum album.Methods: Zebrafish were acclimatised for 7 days, under 28.5 ± 2 °C under a 12-hour light/dark cycle, then bred to collect embryos at the single-cell stage. Embryos were categorised into 8 triplicate groups: unexposed uninfected control, unexposed infected control, alcohol-exposed control, Arsenicum album exposed at 5 potencies (6x, 30c, 200c, 1M, LM/1). Embryos were exposed to medications in egg water medium for four days. From four days post-fertilization (dpf), they were transferred to four-litre tanks. On the seventh day post-exposure (dpe), they were infected with Edwardsiella tarda at 1 × 108 colony forming units (CFU)/ml for five hours. Fishes were collected at 5-hours and 30-hours post-infection (hpi) for assessing innate immunological responses. Survival rates, respiratory bursts and bacterial loads were statistically analysed.Results: The Arsenicum album LM/1 group had the highest survival rate, followed by the 1M group. Highest respiratory burst at 5 hpi and lowest bacterial load at 5 and 30 hpi were in the 1M group. LM/1 had highest respiratory burst at 30 hpi. The 6x group had the lowest survival rate, below the negative control. These findings highlight Arsenicum album's impact on innate immunity in zebrafish embryos.Conclusion: This study emphasizes Arsenicum album's innate immunomodulatory effect across potencies. Subsequent focused research is imperative to acquire a more nuanced comprehension.
{"title":"Immunomodulatory Effect of Arsenicum album on Zebrafish Embryos.","authors":"Akshaya Tharankini, D. L. Prabu, V. W. Vargheese","doi":"10.51910/ijhdr.v22i2.1360","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1360","url":null,"abstract":"Background: Globally, 2.4 million neonates succumbed in 2019, constituting 47% of all under-5 mortalities[1]. India bore the highest neonatal mortality in the same year, attributed predominantly to infections[2]. Innate immunity, bestowed at birth, represents our primary defense against neonatal mortality. Exposure to crude forms of arsenic in zebrafish embryos has been shown to significantly down-regulate the innate immune responses[3-8]. Such studies reveal the realm of operation, susceptible cells, and mechanisms through which arsenic can engage with our system, aiding in the understanding of the Homoeopathic medicine Arsenicum album.Methods: Zebrafish were acclimatised for 7 days, under 28.5 ± 2 °C under a 12-hour light/dark cycle, then bred to collect embryos at the single-cell stage. Embryos were categorised into 8 triplicate groups: unexposed uninfected control, unexposed infected control, alcohol-exposed control, Arsenicum album exposed at 5 potencies (6x, 30c, 200c, 1M, LM/1). Embryos were exposed to medications in egg water medium for four days. From four days post-fertilization (dpf), they were transferred to four-litre tanks. On the seventh day post-exposure (dpe), they were infected with Edwardsiella tarda at 1 × 108 colony forming units (CFU)/ml for five hours. Fishes were collected at 5-hours and 30-hours post-infection (hpi) for assessing innate immunological responses. Survival rates, respiratory bursts and bacterial loads were statistically analysed.Results: The Arsenicum album LM/1 group had the highest survival rate, followed by the 1M group. Highest respiratory burst at 5 hpi and lowest bacterial load at 5 and 30 hpi were in the 1M group. LM/1 had highest respiratory burst at 30 hpi. The 6x group had the lowest survival rate, below the negative control. These findings highlight Arsenicum album's impact on innate immunity in zebrafish embryos.Conclusion: This study emphasizes Arsenicum album's innate immunomodulatory effect across potencies. Subsequent focused research is imperative to acquire a more nuanced comprehension.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"78 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.51910/ijhdr.v22i2.1362
Rodrigo Dos Santos Pinto Duarte, Michelle Nonato de Oliveira Melo, João Vitor da Costa Batista, Giovanna Gomes Martins, Adriana Passos Oliveira, Rosilane Taveira da Silva, Marcelo Einicker-Lamas, Stephan Baumgartner, Carla Holandino
Viscum album (VA) preparations of different host trees have been used for the treatment of different diseases with mainly on cancer1 and cardiovascular diseases2. However, the mechanisms related to the mistletoe hypotensive potential are poorly investigated. Therefore, the objective of this work was to evaluate the cytotoxicity, the Na+/K+ ATPase activity and the Reactive Oxygen Species (ROS) generation induced by winter and summer Viscum album mother tinctures (TMVA), as well as by their potentized samples (6cH, 12cH and 30cH), in porcine proximal tubule renal cells (LLC-PK1). For the cytotoxicity evaluation, after 24h of treatment with TMVA (from Quercus robur, Malus domestica, Ulmus carpinifolia, Pinus sylvestris and Abies alba), at concentrations ranging from 0.5% to 2.5% (V/V), the 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) was added. The same procedure was done with the potentized samples, after systematic control evaluation, in which the dynamized water was blindly tested. For the Na+/K+ ATPase and ROS assays, cells were treated only for 30 min, using 1.5% (V/V) of TMVA of Malus domestica, Pinus sylvestris, and Abies alba and their respective homeopathic potencies. The MTT assay showed a dose-dependent cytotoxicity related only to the summer Quercus Robur TMVA (p<0.05). Since for the enzymatic and ROS assays cells need to be metabolically active, this TMVA was excluded from these experimental evaluations. Regarding the ROS generation (n=5), no significant effects were triggered neither by TMVA nor by the potentized samples (p>0.05). The Na+/K+ ATPase evaluation (n=9) indicated no enzymatic activity alterations at 30min of incubation time. Further experiments are under development with higher incubation cellular times (24 and 48 h) to confirm the Na+/K+ ATPase effects induced by potentized and non-potentized V. album samples. Additionally, the gene expression of Na+/K+ ATPase, by western blot, and the ultrastructural cellular aspects, using transmission electron microscopy, will be performed.
{"title":"Biological effects of Viscum album homeopathic preparations in kidney proximal tubule cells: an in vitro study","authors":"Rodrigo Dos Santos Pinto Duarte, Michelle Nonato de Oliveira Melo, João Vitor da Costa Batista, Giovanna Gomes Martins, Adriana Passos Oliveira, Rosilane Taveira da Silva, Marcelo Einicker-Lamas, Stephan Baumgartner, Carla Holandino","doi":"10.51910/ijhdr.v22i2.1362","DOIUrl":"https://doi.org/10.51910/ijhdr.v22i2.1362","url":null,"abstract":"Viscum album (VA) preparations of different host trees have been used for the treatment of different diseases with mainly on cancer1 and cardiovascular diseases2. However, the mechanisms related to the mistletoe hypotensive potential are poorly investigated. Therefore, the objective of this work was to evaluate the cytotoxicity, the Na+/K+ ATPase activity and the Reactive Oxygen Species (ROS) generation induced by winter and summer Viscum album mother tinctures (TMVA), as well as by their potentized samples (6cH, 12cH and 30cH), in porcine proximal tubule renal cells (LLC-PK1). For the cytotoxicity evaluation, after 24h of treatment with TMVA (from Quercus robur, Malus domestica, Ulmus carpinifolia, Pinus sylvestris and Abies alba), at concentrations ranging from 0.5% to 2.5% (V/V), the 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) was added. The same procedure was done with the potentized samples, after systematic control evaluation, in which the dynamized water was blindly tested. For the Na+/K+ ATPase and ROS assays, cells were treated only for 30 min, using 1.5% (V/V) of TMVA of Malus domestica, Pinus sylvestris, and Abies alba and their respective homeopathic potencies. The MTT assay showed a dose-dependent cytotoxicity related only to the summer Quercus Robur TMVA (p<0.05). Since for the enzymatic and ROS assays cells need to be metabolically active, this TMVA was excluded from these experimental evaluations. Regarding the ROS generation (n=5), no significant effects were triggered neither by TMVA nor by the potentized samples (p>0.05). The Na+/K+ ATPase evaluation (n=9) indicated no enzymatic activity alterations at 30min of incubation time. Further experiments are under development with higher incubation cellular times (24 and 48 h) to confirm the Na+/K+ ATPase effects induced by potentized and non-potentized V. album samples. Additionally, the gene expression of Na+/K+ ATPase, by western blot, and the ultrastructural cellular aspects, using transmission electron microscopy, will be performed.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-16DOI: 10.51910/ijhdr.v22icf.1295
Sreeja Kudukayil
Background: Schizophrenia is a mental condition not only affecting routine of daily of life but also the Quality of life with devastating consequences for affected individuals and their families.Objectives: This review aims to detect the current status of homoeopathy in the management of schizophrenia. Materials and Method: a search is done in search engines of Google scholar, PubMed and Cochrane, CINAHL PsycINFO, Hom-Inform. Identified articles of homoeopathy in schizophrenia is collected and summarised in this review. Result: Though there is scarcity in studies, the available data shows scope for future research in homoeopathy in schizophrenia.
背景：精神分裂症是一种精神疾病，不仅影响日常生活，还影响生活质量，给患者及其家人带来毁灭性后果：本综述旨在了解同种疗法在治疗精神分裂症方面的现状。材料与方法：在 Google scholar、PubMed 和 Cochrane、CINAHL PsycINFO、Hom-Inform 等搜索引擎中进行了搜索。本综述收集并总结了所发现的有关同种疗法治疗精神分裂症的文章。结果：虽然研究数量稀少，但现有数据显示了同种疗法在精神分裂症方面的未来研究空间。
{"title":"A review on the current status of homoeopathy in the management of schizophrenia","authors":"Sreeja Kudukayil","doi":"10.51910/ijhdr.v22icf.1295","DOIUrl":"https://doi.org/10.51910/ijhdr.v22icf.1295","url":null,"abstract":"Background: Schizophrenia is a mental condition not only affecting routine of daily of life but also the Quality of life with devastating consequences for affected individuals and their families.Objectives: This review aims to detect the current status of homoeopathy in the management of schizophrenia. Materials and Method: a search is done in search engines of Google scholar, PubMed and Cochrane, CINAHL PsycINFO, Hom-Inform. Identified articles of homoeopathy in schizophrenia is collected and summarised in this review. Result: Though there is scarcity in studies, the available data shows scope for future research in homoeopathy in schizophrenia.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"36 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138967130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-16DOI: 10.51910/ijhdr.v22icf.1234
D. Gilla, Sreeja Kr, Sreelakshmy Sr, Rajakumar Bsj
Background: Emotional and behavioural disorders are very common in childhood and adolescence. Homoeopathy is an alternative system of medicine which has a potential role in Child and Adolescent Psychiatry (CAP). Objectives: To identify, summarize and disseminate research findings that could assess the role of Homoeopathy in CAP. Materials and Methods: Two electronic databases PubMed and Google scholar are searched for publications on childhood behavioural disorders in peer-reviewed, indexed journals. Articles published on Homoeopathy and CAP in the past two decades i.e from 2000 to 2020 are included. An explicit systematic search strategy included web-based key word searches. The collected information has been collated, summarized, and the results are presented in tables and charts according to key themes. Results: The research studies varied widely in all aspects such as design, conduct, intervention and outcome reporting. 38 eligible studies are identified on different clinical conditions in CAP. Individual sources of data are critically appraised and findings are discussed in terms of implications for gaps in research. Conclusions: There is limited yet convincing evidence for usefulness of Homoeopathy in CAP disorders such as Autism and ADHD. Our review emphasises the paucity of studies in other CAP disorders such as Intellectual disability, Learning disability, Conduct disorder, Childhood depression and Anxiety disorders etc. which is the domain of future research.
{"title":"Review of the Literature on Usefulness of Homoeopathy in Child and Adolescent Psychiatry","authors":"D. Gilla, Sreeja Kr, Sreelakshmy Sr, Rajakumar Bsj","doi":"10.51910/ijhdr.v22icf.1234","DOIUrl":"https://doi.org/10.51910/ijhdr.v22icf.1234","url":null,"abstract":"Background: Emotional and behavioural disorders are very common in childhood and adolescence. Homoeopathy is an alternative system of medicine which has a potential role in Child and Adolescent Psychiatry (CAP). \u0000Objectives: To identify, summarize and disseminate research findings that could assess the role of Homoeopathy in CAP. \u0000Materials and Methods: Two electronic databases PubMed and Google scholar are searched for publications on childhood behavioural disorders in peer-reviewed, indexed journals. Articles published on Homoeopathy and CAP in the past two decades i.e from 2000 to 2020 are included. An explicit systematic search strategy included web-based key word searches. The collected information has been collated, summarized, and the results are presented in tables and charts according to key themes. \u0000Results: The research studies varied widely in all aspects such as design, conduct, intervention and outcome reporting. 38 eligible studies are identified on different clinical conditions in CAP. Individual sources of data are critically appraised and findings are discussed in terms of implications for gaps in research. \u0000Conclusions: There is limited yet convincing evidence for usefulness of Homoeopathy in CAP disorders such as Autism and ADHD. Our review emphasises the paucity of studies in other CAP disorders such as Intellectual disability, Learning disability, Conduct disorder, Childhood depression and Anxiety disorders etc. which is the domain of future research.","PeriodicalId":106057,"journal":{"name":"International Journal of High Dilution Research - ISSN 1982-6206","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127075749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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