Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n1a10
G. Parrella, E. Troiano
Tomato is the second most important vegetable crop in the world after potato. Its production is hindered by fungal and viral diseases. Control of these diseases requires a significant investment that represents up to 40% of the production costs. Marker-assisted gene pyramiding is a powerful breeding tool which allow the rapid constitution of breeding lines with broad spectrum of resistance against diseases. Nevertheless, the accuracy and rapidity of introgression of genes of interest are some of the most critical barriers to successful application. In this paper, we use a simple and rapid method that combines duplex PCR for two important virus resistance genes (i.e., Sw-5 and Tm-22) and uses the linkage between genes Tm-22 and Forl to speed the introgression of these resistance genes into a “Pomodoro di Sorrento” tomato line. The method was used through six generations of backcrossing followed by three generations of selfing and resulted in a homozygous resistant line.
{"title":"Pyramiding disease resistance in tomato by duplex PCR targeting resistance genes and exploiting gene linkage","authors":"G. Parrella, E. Troiano","doi":"10.1590/1984-70332022v22n1a10","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n1a10","url":null,"abstract":"Tomato is the second most important vegetable crop in the world after potato. Its production is hindered by fungal and viral diseases. Control of these diseases requires a significant investment that represents up to 40% of the production costs. Marker-assisted gene pyramiding is a powerful breeding tool which allow the rapid constitution of breeding lines with broad spectrum of resistance against diseases. Nevertheless, the accuracy and rapidity of introgression of genes of interest are some of the most critical barriers to successful application. In this paper, we use a simple and rapid method that combines duplex PCR for two important virus resistance genes (i.e., Sw-5 and Tm-22) and uses the linkage between genes Tm-22 and Forl to speed the introgression of these resistance genes into a “Pomodoro di Sorrento” tomato line. The method was used through six generations of backcrossing followed by three generations of selfing and resulted in a homozygous resistant line.","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n4a50
J. L. Ferreira, J. Kami, A. Borém, P. Gepts
{"title":"Sequence Analysis of the Arcelin-Phytohaemagglutinin-α-Amylase Inhibitor (APA) Locus in Three Phylogenetically Arrayed Phaseolus vulgaris Clones","authors":"J. L. Ferreira, J. Kami, A. Borém, P. Gepts","doi":"10.1590/1984-70332022v22n4a50","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n4a50","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n4c38
E. Vieira, Marco Antonio Sedrez Rangel, J. F. Fialho, R. Ringenberg, M. Romano, M. Rinaldi, Charles Martins de Oliveira, J. Antonini
{"title":"BRS 429: sweet cassava with yellow pulp and high technological and sensory qualities","authors":"E. Vieira, Marco Antonio Sedrez Rangel, J. F. Fialho, R. Ringenberg, M. Romano, M. Rinaldi, Charles Martins de Oliveira, J. Antonini","doi":"10.1590/1984-70332022v22n4c38","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n4c38","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n2c17
R. F. Vieira, J. Carneiro, T. J. P. Paula Júnior, R. C. Lima, B. A. Soares, P. H. Teixeira, Davi Moreira Santos, L. L. Jacoob
: ‘BRSMG Camaleão’ is a mungbean cultivar with large, shiny, green seeds released for the state of Minas Gerais, Brazil. It was developed by the Asian Vegetable Research and Development Center as line VC 6148-B-16. In seven trials established in the Zona da Mata region, it had an average yield of 1493 kg ha -1 and 1000-seed weight of 68-78 g.
' BRSMG camale o '是一种绿豆品种，种子大，有光泽，绿色，为巴西米纳斯吉拉斯州发布。它是由亚洲蔬菜研究与发展中心开发的，品系为VC 6148-B-16。在Zona da Mata地区进行的7次试验中，它的平均产量为1493公斤/公顷，千粒重为68-78克。
{"title":"BRSMG Camaleão: new mungbean cultivar with large, shiny, green seeds","authors":"R. F. Vieira, J. Carneiro, T. J. P. Paula Júnior, R. C. Lima, B. A. Soares, P. H. Teixeira, Davi Moreira Santos, L. L. Jacoob","doi":"10.1590/1984-70332022v22n2c17","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n2c17","url":null,"abstract":": ‘BRSMG Camaleão’ is a mungbean cultivar with large, shiny, green seeds released for the state of Minas Gerais, Brazil. It was developed by the Asian Vegetable Research and Development Center as line VC 6148-B-16. In seven trials established in the Zona da Mata region, it had an average yield of 1493 kg ha -1 and 1000-seed weight of 68-78 g.","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n4c41
V. Marchioro, D. Meira, Luís Antônio Klein, Carine Meier, V. Q. Souza, D. N. Follmann, I. Carvalho, B. O. Caron, D. Schimidt, M. Nardino, Tiago Olivoto
{"title":"UFSMFW 2202 - Early black oat cultivar for ground cover","authors":"V. Marchioro, D. Meira, Luís Antônio Klein, Carine Meier, V. Q. Souza, D. N. Follmann, I. Carvalho, B. O. Caron, D. Schimidt, M. Nardino, Tiago Olivoto","doi":"10.1590/1984-70332022v22n4c41","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n4c41","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n4a42
F. D. Sánchez-Mora, L. Saifert, J. Zanghelini, C. A. Paixão, L. L. D. Vesco, R. Eibach, M. A. Dalbó, R. Nodari, L. Welter
{"title":"Pyramiding of resistance alleles to grape powdery mildew assisted by molecular markers","authors":"F. D. Sánchez-Mora, L. Saifert, J. Zanghelini, C. A. Paixão, L. L. D. Vesco, R. Eibach, M. A. Dalbó, R. Nodari, L. Welter","doi":"10.1590/1984-70332022v22n4a42","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n4a42","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n4a43
R. H. Fernandes, D. Silva, F. T. Delazari, E. A. Lopes
{"title":"Screening of tomato hybrids for resistance to Fusarium wilt","authors":"R. H. Fernandes, D. Silva, F. T. Delazari, E. A. Lopes","doi":"10.1590/1984-70332022v22n4a43","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n4a43","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n1a07
P. C. D. Fonseca, D. Ferreira, Túlio Morgan, T. Mendes, F. L. Silva
Bacterial pustule is an important soybean disease caused by Xanthomonas citri pv. glycines, but information about genetic resistance to this pathogen is scarce. This study aimed to investigate soybean genetic resistance to bacterial pustule in the flowering stage through association analysis, characterization of candidate SNP markers, and identification of protein-coding genes potentially regulating defense processes. Therefore, 109 soybean cultivars genotyped with a 6k Illumina platform were assessed for disease severity. A genome-wide analysis revealed a total of 13 SNPs significantly associated. Through protein annotation, we identified three markers located inside the coding regions of uncharacterized protein LOC100779077, histone-lysine Nmethyltransferase SUVR4, and ABC-transporter B family member-9. Nucleotide polymorphism on the first two of these markers produces a non-synonymous polymorphism with polarity shift from hydrophobic to polar amino acid. It is convenient to prioritize these three candidate markers for validation procedures with the purpose of using them in marker-assisted soybean breeding programs.
细菌性脓疱是柑橘黄单胞菌引起的一种重要的大豆病害。甘氨酸，但关于这种病原体的遗传抗性的信息很少。本研究旨在通过关联分析、候选SNP标记的鉴定和鉴定可能调节防御过程的蛋白质编码基因，研究大豆在开花阶段对细菌性脓疱的遗传抗性。因此，用6k Illumina平台对109个大豆品种进行了疾病严重程度的评估。全基因组分析显示，共有13个snp显著相关。通过蛋白质注释，我们确定了位于未鉴定蛋白LOC100779077、组蛋白赖氨酸Nmethyltransferase SUVR4和ABC-transporter B family member-9编码区内的三个标记。前两个标记上的核苷酸多态性产生了从疏水氨基酸到极性氨基酸的非同义多态性。为了便于在标记辅助大豆育种计划中使用这三个候选标记，对验证程序进行优先排序是方便的。
{"title":"Causal variant loci and protein-coding genes for soybean bacterial pustule resistance in the flowering stage","authors":"P. C. D. Fonseca, D. Ferreira, Túlio Morgan, T. Mendes, F. L. Silva","doi":"10.1590/1984-70332022v22n1a07","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n1a07","url":null,"abstract":"Bacterial pustule is an important soybean disease caused by Xanthomonas citri pv. glycines, but information about genetic resistance to this pathogen is scarce. This study aimed to investigate soybean genetic resistance to bacterial pustule in the flowering stage through association analysis, characterization of candidate SNP markers, and identification of protein-coding genes potentially regulating defense processes. Therefore, 109 soybean cultivars genotyped with a 6k Illumina platform were assessed for disease severity. A genome-wide analysis revealed a total of 13 SNPs significantly associated. Through protein annotation, we identified three markers located inside the coding regions of uncharacterized protein LOC100779077, histone-lysine Nmethyltransferase SUVR4, and ABC-transporter B family member-9. Nucleotide polymorphism on the first two of these markers produces a non-synonymous polymorphism with polarity shift from hydrophobic to polar amino acid. It is convenient to prioritize these three candidate markers for validation procedures with the purpose of using them in marker-assisted soybean breeding programs.","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67261562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n3a28
Danilo Alves Pereira, R. Parrella, Carlos Roberto Silva de Oliveira, J. A. R. Nunes
{"title":"Agroindustrial performance and heterosis in sweet sorghum using male-sterile lines with high stem sugar content","authors":"Danilo Alves Pereira, R. Parrella, Carlos Roberto Silva de Oliveira, J. A. R. Nunes","doi":"10.1590/1984-70332022v22n3a28","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n3a28","url":null,"abstract":"","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/1984-70332022v22n1c04
F. F. Teixeira, Israel Alexandre Pereira Filho, W. F. Meirelles, Jane Rodrigues de Assis Machado, A. Portugal, Luciano Viana Cotta
: BRS Deni is a super-sweet corn open-pollinated variety, developed and released by the Brazilian Agricultural Research Corporation (Embrapa) that combines high yield and good ear and grain quality and lower production costs. The grain can be consumed fresh or canned. over three generations for recombination and selection for yield, quality and agronomic traits. The last selection in With the recombined generations, the variety was tested in trials to evaluate yield, quality and agronomics traits, using the hybrids BRS Vivi, Tropical Plus and SWB 551 as checks. The evaluation trials were conducted in: Sete Lagoas/2015 (L1), Londrina/2015 (L2), Sete Lagoas/2016 (L3), Janaúba/2016 (L4), Londrina/2017 (L5), and Passo Fundo/2017 (L6) in a randomized block design with three replications and experimental plots with four 5-m rows, spaced 80 cm apart. In these trials, the following traits were evaluated: number of days to female flowering (FF), from seedling germination until 50% of the plants in the plot emit style-stigmas; plant height (PH, cm) and first ear height (EH, cm); prolificity (PR), based on the ratio of number of ears to number of plants per plot; grain yield at maturity (GY, t ha -1 ); unhusked ear yield of green corn (EY, tons/ha); ear length (LE, cm) and diameter (DE, mm) of unhusked ears at the green corn stage; number of grain rows per ear (NR); mean ear weight (EW, g); grain weight per ear (GW, g) at the green corn stage; and brix degrees (BR) computed as the mean of two samples of ground grains measured with a portable digital refractometer (Atago PALM-1). Analysis of variance and the Tukey mean test were performed using software GENES (Cruz 2006).
{"title":"BRS Deni - Super-sweet corn open-pollinated variety","authors":"F. F. Teixeira, Israel Alexandre Pereira Filho, W. F. Meirelles, Jane Rodrigues de Assis Machado, A. Portugal, Luciano Viana Cotta","doi":"10.1590/1984-70332022v22n1c04","DOIUrl":"https://doi.org/10.1590/1984-70332022v22n1c04","url":null,"abstract":": BRS Deni is a super-sweet corn open-pollinated variety, developed and released by the Brazilian Agricultural Research Corporation (Embrapa) that combines high yield and good ear and grain quality and lower production costs. The grain can be consumed fresh or canned. over three generations for recombination and selection for yield, quality and agronomic traits. The last selection in With the recombined generations, the variety was tested in trials to evaluate yield, quality and agronomics traits, using the hybrids BRS Vivi, Tropical Plus and SWB 551 as checks. The evaluation trials were conducted in: Sete Lagoas/2015 (L1), Londrina/2015 (L2), Sete Lagoas/2016 (L3), Janaúba/2016 (L4), Londrina/2017 (L5), and Passo Fundo/2017 (L6) in a randomized block design with three replications and experimental plots with four 5-m rows, spaced 80 cm apart. In these trials, the following traits were evaluated: number of days to female flowering (FF), from seedling germination until 50% of the plants in the plot emit style-stigmas; plant height (PH, cm) and first ear height (EH, cm); prolificity (PR), based on the ratio of number of ears to number of plants per plot; grain yield at maturity (GY, t ha -1 ); unhusked ear yield of green corn (EY, tons/ha); ear length (LE, cm) and diameter (DE, mm) of unhusked ears at the green corn stage; number of grain rows per ear (NR); mean ear weight (EW, g); grain weight per ear (GW, g) at the green corn stage; and brix degrees (BR) computed as the mean of two samples of ground grains measured with a portable digital refractometer (Atago PALM-1). Analysis of variance and the Tukey mean test were performed using software GENES (Cruz 2006).","PeriodicalId":10763,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67262204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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