Pub Date : 2026-01-21DOI: 10.1016/j.copbio.2026.103437
Taku Mizutani , Ikuro Abe
Noncanonical amino acids (ncAAs) are pharmaceutically important molecules with diverse biological and chemical applications. Among various production strategies, biocatalytic synthesis using pyridoxal 5′-phosphate (PLP)-dependent enzymes has attracted considerable attention owing to their high stereoselectivity and versatility in forming new C–C bonds. Recent advances in enzyme engineering have further expanded the catalytic potential of PLP-dependent enzymes, enabling the tailored synthesis of target ncAAs. In this short review, we summarize recent developments in biocatalytic ncAA synthesis achieved through engineered PLP-dependent enzymes.
{"title":"Expanding the catalytic repertoire of C–C bond–forming pyridoxal 5′-phosphate-dependent enzymes for noncanonical amino acid formation","authors":"Taku Mizutani , Ikuro Abe","doi":"10.1016/j.copbio.2026.103437","DOIUrl":"10.1016/j.copbio.2026.103437","url":null,"abstract":"<div><div>Noncanonical amino acids (ncAAs) are pharmaceutically important molecules with diverse biological and chemical applications. Among various production strategies, biocatalytic synthesis using pyridoxal 5′-phosphate (PLP)-dependent enzymes has attracted considerable attention owing to their high stereoselectivity and versatility in forming new C–C bonds. Recent advances in enzyme engineering have further expanded the catalytic potential of PLP-dependent enzymes, enabling the tailored synthesis of target ncAAs. In this short review, we summarize recent developments in biocatalytic ncAA synthesis achieved through engineered PLP-dependent enzymes.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103437"},"PeriodicalIF":7.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-20DOI: 10.1016/j.copbio.2026.103438
Ximin Piao , Megan L Matthews
Mathematical models that simulate crop growth in response to environmental conditions and management practices are essential tools for exploring agriculture-based strategies to address food security and environmental sustainability challenges. Early applications of crop models focused on supporting farmers in making management decisions. Applications have since expanded to estimating future impacts on local and global food production from changing climates. Emerging applications of crop models aim to leverage how these models integrate plant processes across biological scales to identify engineering or breeding strategies that account for environmentally-responsive dynamics at field scales and for exploring solutions to improve sustainability. In this review, we highlight recent studies across these four broad application areas and highlight potential future directions for the crop modeling field.
{"title":"Crop models: integrating systems from the molecular to global for agricultural productivity and sustainability","authors":"Ximin Piao , Megan L Matthews","doi":"10.1016/j.copbio.2026.103438","DOIUrl":"10.1016/j.copbio.2026.103438","url":null,"abstract":"<div><div>Mathematical models that simulate crop growth in response to environmental conditions and management practices are essential tools for exploring agriculture-based strategies to address food security and environmental sustainability challenges. Early applications of crop models focused on supporting farmers in making management decisions. Applications have since expanded to estimating future impacts on local and global food production from changing climates. Emerging applications of crop models aim to leverage how these models integrate plant processes across biological scales to identify engineering or breeding strategies that account for environmentally-responsive dynamics at field scales and for exploring solutions to improve sustainability. In this review, we highlight recent studies across these four broad application areas and highlight potential future directions for the crop modeling field.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103438"},"PeriodicalIF":7.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-19DOI: 10.1016/j.copbio.2025.103435
Rachel Armstrong
This review explores the reconceptualisation of microbial colonisation on buildings: from a detrimental process (biofouling) to a source of beneficial, programmable biocatalysis. Strategies for embedding microbial and fungal communities into architectural materials to perform functions such as bioremediation, biomineralisation, and energy generation are explored. The analysis includes the multiscalar design of bioreceptive substrates, engineered living paints, mycelium composites, and probiotic surfaces, which transform passive structures into metabolically active interfaces. These approaches are regarded as Engineered Eco-Ornamentation, where surface design intentionally supports microbial ecology and urban metabolism. The integration of these living systems with computational modelling and digital fabrication to create adaptive building systems is considered. Key challenges include scaling biological processes for architectural application, ensuring long-term material durability, and aligning metabolic activity with practical construction constraints. Addressing these challenges positions functionally designed biocatalytic surfaces as a foundational research field for more regenerative and ecologically integrated architecture.
{"title":"Biocatalytic surfaces in architecture","authors":"Rachel Armstrong","doi":"10.1016/j.copbio.2025.103435","DOIUrl":"10.1016/j.copbio.2025.103435","url":null,"abstract":"<div><div>This review explores the reconceptualisation of microbial colonisation on buildings: from a detrimental process (biofouling) to a source of beneficial, programmable biocatalysis. Strategies for embedding microbial and fungal communities into architectural materials to perform functions such as bioremediation, biomineralisation, and energy generation are explored. The analysis includes the multiscalar design of bioreceptive substrates, engineered living paints, mycelium composites, and probiotic surfaces, which transform passive structures into metabolically active interfaces. These approaches are regarded as <em>Engineered Eco-Ornamentation</em>, where surface design intentionally supports microbial ecology and urban metabolism. The integration of these living systems with computational modelling and digital fabrication to create adaptive building systems is considered. Key challenges include scaling biological processes for architectural application, ensuring long-term material durability, and aligning metabolic activity with practical construction constraints. Addressing these challenges positions functionally designed biocatalytic surfaces as a foundational research field for more regenerative and ecologically integrated architecture.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103435"},"PeriodicalIF":7.0,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-14DOI: 10.1016/j.copbio.2025.103432
Yoshiahu Goldstein, Guy Polturak
Plant natural products (PNPs) are specialized metabolites with diverse biological activities that are central to plant adaptation to biotic stresses. They act as chemical defenses against pathogens and pests, supporting plant survival under attack. Harnessing their potential as eco-friendly biopesticides requires a detailed understanding of their biosynthetic pathways and biological functions. This review highlights recent advances in the discovery and pathway elucidation of defensive PNPs and discusses strategies for their application through heterologous expression and enhanced in planta production.
{"title":"Harnessing plant natural products for enhanced biotic stress resistance","authors":"Yoshiahu Goldstein, Guy Polturak","doi":"10.1016/j.copbio.2025.103432","DOIUrl":"10.1016/j.copbio.2025.103432","url":null,"abstract":"<div><div>Plant natural products (PNPs) are specialized metabolites with diverse biological activities that are central to plant adaptation to biotic stresses. They act as chemical defenses against pathogens and pests, supporting plant survival under attack. Harnessing their potential as eco-friendly biopesticides requires a detailed understanding of their biosynthetic pathways and biological functions. This review highlights recent advances in the discovery and pathway elucidation of defensive PNPs and discusses strategies for their application through heterologous expression and enhanced <em>in planta</em> production.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103432"},"PeriodicalIF":7.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.copbio.2025.103433
Hui Ren , Venkatesan Sundaresan
Harnessing heterosis has been important to agriculture, but its full potential remains constrained by the inability to clonally propagate hybrid seeds. Synthetic apomixis, engineering asexual seed formation in sexual crops, provides a path to fix heterosis through generations. This review compiles current advances in engineering synthetic apomixis, focusing on two major components: the substitution of mitosis for meiotic divisions and maternally derived embryo development by haploid inducer or parthenogenesis. Despite rapid progress made in rice, significant challenges remain for general implementation and extension to other crops. We discuss these challenges and the possible paths forward to make synthetic apomixis feasible for widespread application in agriculture.
{"title":"Strategies and challenges for synthetic apomixis","authors":"Hui Ren , Venkatesan Sundaresan","doi":"10.1016/j.copbio.2025.103433","DOIUrl":"10.1016/j.copbio.2025.103433","url":null,"abstract":"<div><div>Harnessing heterosis has been important to agriculture, but its full potential remains constrained by the inability to clonally propagate hybrid seeds. Synthetic apomixis, engineering asexual seed formation in sexual crops, provides a path to fix heterosis through generations. This review compiles current advances in engineering synthetic apomixis, focusing on two major components: the substitution of mitosis for meiotic divisions and maternally derived embryo development by haploid inducer or parthenogenesis. Despite rapid progress made in rice, significant challenges remain for general implementation and extension to other crops. We discuss these challenges and the possible paths forward to make synthetic apomixis feasible for widespread application in agriculture.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103433"},"PeriodicalIF":7.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.copbio.2025.103434
Lea M Sommer, Teddy Groves, Alberto Santos
Artificial intelligence (AI) and machine learning are reshaping biotechnology, but their impact is still constrained by inadequate data infrastructure. Although recent advances have focused on model architectures, data quality, standardization, and interoperability remain the main bottlenecks. A data-centric approach that strengthens data practices across the design-build-test-learn cycle is needed to advance AI-enabled biomanufacturing. In this review, we argue that curated repositories, consistent metadata, real-time validation, and efficient learning strategies are essential prerequisites. We highlight the manual test-to-learn ingestion step as a critical source of latency that can be mitigated through semantic standardization and structured data collection. By addressing these data-centric limitations, the field can move toward reliable, scalable, and ultimately autonomous virtual laboratories that adhere to the Findable, Accessible, Interoperable, Reusable principles.
{"title":"Beyond the model: data infrastructure as the foundation for autonomous virtual laboratories","authors":"Lea M Sommer, Teddy Groves, Alberto Santos","doi":"10.1016/j.copbio.2025.103434","DOIUrl":"10.1016/j.copbio.2025.103434","url":null,"abstract":"<div><div>Artificial intelligence (AI) and machine learning are reshaping biotechnology, but their impact is still constrained by inadequate data infrastructure. Although recent advances have focused on model architectures, data quality, standardization, and interoperability remain the main bottlenecks. A data-centric approach that strengthens data practices across the design-build-test-learn cycle is needed to advance AI-enabled biomanufacturing. In this review, we argue that curated repositories, consistent metadata, real-time validation, and efficient learning strategies are essential prerequisites. We highlight the manual test-to-learn ingestion step as a critical source of latency that can be mitigated through semantic standardization and structured data collection. By addressing these data-centric limitations, the field can move toward reliable, scalable, and ultimately autonomous virtual laboratories that adhere to the Findable, Accessible, Interoperable, Reusable principles.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103434"},"PeriodicalIF":7.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.copbio.2025.103431
Anjali Kharb , Xuejun Zhu
The human gut microbiota, particularly the intestinal microbiota, shapes host physiology, disease risk, and therapeutic outcomes through complex metabolic and enzymatic activities. Recent advances in molecular omics, metabolomics, enzyme bioinformatics, and artificial intelligence (AI) have created unprecedented opportunities to elucidate its therapeutic roles to further enable precision microbiome medicine for personalized prevention, diagnosis, and treatment. In this review, we highlight emerging applications that leverage molecular omics and metabolomics technologies to dissect gut microbial functions, along with developments in enzyme bioinformatics and AI tools that reveal gut microbial species, enzymes, and metabolic pathways impacting human health. Finally, we discuss perspectives on data standardization, functional annotation, and interpretability, and how emerging tools are accelerating translational microbiome research.
{"title":"Unlocking therapeutic impacts of the gut microbiota with computational tools","authors":"Anjali Kharb , Xuejun Zhu","doi":"10.1016/j.copbio.2025.103431","DOIUrl":"10.1016/j.copbio.2025.103431","url":null,"abstract":"<div><div>The human gut microbiota, particularly the intestinal microbiota, shapes host physiology, disease risk, and therapeutic outcomes through complex metabolic and enzymatic activities. Recent advances in molecular omics, metabolomics, enzyme bioinformatics, and artificial intelligence (AI) have created unprecedented opportunities to elucidate its therapeutic roles to further enable precision microbiome medicine for personalized prevention, diagnosis, and treatment. In this review, we highlight emerging applications that leverage molecular omics and metabolomics technologies to dissect gut microbial functions, along with developments in enzyme bioinformatics and AI tools that reveal gut microbial species, enzymes, and metabolic pathways impacting human health. Finally, we discuss perspectives on data standardization, functional annotation, and interpretability, and how emerging tools are accelerating translational microbiome research.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103431"},"PeriodicalIF":7.0,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.copbio.2025.103429
Hailong Guo , Jonathan D.G. Jones
Plants employ cell-surface and intracellular immune receptors to perceive pathogens and activate defense responses. Recent advances in mechanistic understanding of how cell-surface and intracellular immune receptors convert recognition of molecular patterns or effectors into defense activation, combined with the knowledge of receptor repertoire variation both within and between species, allow transfer of immune receptors between species to increase the spectrum of recognition specificities. Here, we summarize recent progress in the functional transfer of immune receptors within and between plant families. We also discuss challenges that limit the transferability of intracellular immune receptors, including the requirement of additional host factors or downstream components and their incompatibility between donor and recipient species. Finally, we provide an overview of future perspectives for bioengineering disease-resistant crops through immune receptor transfer.
{"title":"PRRs and NLRs sans frontières: advances and challenges in transfer of immune receptors between plant species","authors":"Hailong Guo , Jonathan D.G. Jones","doi":"10.1016/j.copbio.2025.103429","DOIUrl":"10.1016/j.copbio.2025.103429","url":null,"abstract":"<div><div>Plants employ cell-surface and intracellular immune receptors to perceive pathogens and activate defense responses. Recent advances in mechanistic understanding of how cell-surface and intracellular immune receptors convert recognition of molecular patterns or effectors into defense activation, combined with the knowledge of receptor repertoire variation both within and between species, allow transfer of immune receptors between species to increase the spectrum of recognition specificities. Here, we summarize recent progress in the functional transfer of immune receptors within and between plant families. We also discuss challenges that limit the transferability of intracellular immune receptors, including the requirement of additional host factors or downstream components and their incompatibility between donor and recipient species. Finally, we provide an overview of future perspectives for bioengineering disease-resistant crops through immune receptor transfer.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103429"},"PeriodicalIF":7.0,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.copbio.2025.103430
Ajay Gupta , Priti Sharma , Bing Yang
Bacterial type III effector proteins, particularly transcription activator-like effectors (TALEs) secreted by Xanthomonas spp., play critical roles in pathogen–host dynamics. While TALEs facilitate bacterial infections, they also possess vulnerabilities that plants and scientists can exploit to develop mechanisms of resistance. This review encompasses the characteristics and functions of TALEs, examining both their virulence and avirulence roles, and the host plants’ counter-strategies. We highlight advancements in genome editing technologies aimed at combating TALE-dependent plant diseases, with a focus on bacterial blight and leaf streak of rice, but also including bacterial blights of cotton and cassava, and citrus canker. Additionally, we share perspectives on various strategies and approaches for applying genome editing tools to improve disease resistance traits in crop breeding.
{"title":"Reprogramming immunity: TAL effector-informed genome editing in rice and other crops","authors":"Ajay Gupta , Priti Sharma , Bing Yang","doi":"10.1016/j.copbio.2025.103430","DOIUrl":"10.1016/j.copbio.2025.103430","url":null,"abstract":"<div><div>Bacterial type III effector proteins, particularly transcription activator-like effectors (TALEs) secreted by <em>Xanthomonas</em> spp., play critical roles in pathogen–host dynamics. While TALEs facilitate bacterial infections, they also possess vulnerabilities that plants and scientists can exploit to develop mechanisms of resistance. This review encompasses the characteristics and functions of TALEs, examining both their virulence and avirulence roles, and the host plants’ counter-strategies. We highlight advancements in genome editing technologies aimed at combating TALE-dependent plant diseases, with a focus on bacterial blight and leaf streak of rice, but also including bacterial blights of cotton and cassava, and citrus canker. Additionally, we share perspectives on various strategies and approaches for applying genome editing tools to improve disease resistance traits in crop breeding.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103430"},"PeriodicalIF":7.0,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-10DOI: 10.1016/j.copbio.2025.103427
Hiroyuki J Kanaya , Koji L Ode , Hiroki R Ueda
Sleep, a universal biological phenomenon, is regulated by multiscale processes, from molecular mechanisms to cellular networks. While the underlying mechanisms, particularly those governing sleep homeostasis, were poorly understood, recent technological breakthroughs have facilitated the identification of molecular and circuit-based mechanisms. Advances in mouse genetics, including next-generation genetics that bypass the need for crossing and postnatal gene knockout methods, enable the comprehensive identification of molecular components for sleep regulation by combining them with noninvasive, large-scale sleep measurements. Elucidated mechanisms include Ca2+-related and protein kinase/phosphatase-mediated signaling, supporting the phosphorylation hypothesis of sleep. The molecular signaling forms ‘cellular sleepiness’ in sleep regulatory neurons to modulate neuronal activity. These integrated understandings of multiscale mechanisms will lead to a system-level understanding of sleep regulation.
{"title":"The systems biology of sleep: toward integrative understanding of molecular and circuit-based mechanisms of sleep","authors":"Hiroyuki J Kanaya , Koji L Ode , Hiroki R Ueda","doi":"10.1016/j.copbio.2025.103427","DOIUrl":"10.1016/j.copbio.2025.103427","url":null,"abstract":"<div><div>Sleep, a universal biological phenomenon, is regulated by multiscale processes, from molecular mechanisms to cellular networks. While the underlying mechanisms, particularly those governing sleep homeostasis, were poorly understood, recent technological breakthroughs have facilitated the identification of molecular and circuit-based mechanisms. Advances in mouse genetics, including next-generation genetics that bypass the need for crossing and postnatal gene knockout methods, enable the comprehensive identification of molecular components for sleep regulation by combining them with noninvasive, large-scale sleep measurements. Elucidated mechanisms include Ca<sup>2+</sup>-related and protein kinase/phosphatase-mediated signaling, supporting the phosphorylation hypothesis of sleep. The molecular signaling forms ‘cellular sleepiness’ in sleep regulatory neurons to modulate neuronal activity. These integrated understandings of multiscale mechanisms will lead to a system-level understanding of sleep regulation.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103427"},"PeriodicalIF":7.0,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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