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Impact of plant additives: Parsley (Petroselinum crispum) leaves and red bell pepper (Capsicum annuum) on the quality of eggless wheat pasta 植物添加剂:欧芹叶和红甜椒对无蛋小麦面食品质的影响
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-07-11 DOI: 10.17221/206/2021-cjfs
Eugenia Covaliov, O. Deseatnicova, Vladislav Resitca, Natalia Suhodol, G. Carolina, Siminiuc Rodica
Pasta is very popular among different groups of the population, being a healthy and cheap product. Therefore, pasta is a promising object for its enrichment with functional ingredients. The paper examined the possibility and feasibility of using red bell pepper powder (BPP) and parsley leaf powder (PLP) in order to enhance the pasta biological value. Recipes of spaghetti pasta production with the addition of red BPP and PLP were developed. The effect of powders from red bell pepper and parsley leaf incorporation on the physicochemical and culinary properties of pasta was studied. The enrichment of pasta induced a decrease in optimal cooking time, swelling index (SI) and water absorption index (WAI). The addition of plant powders decreased the lightness of pasta significantly (P < 0.05) compared to the control sample. On the other hand, the addition of vegetable powders has a positive influence on the total polyphenol content (TPC) and antioxidant activity of pasta. In enriched pasta, the polyphenol content has doubled (pasta with 10.0% BPP) or even tripled (pasta with 10.0% PLP). The received scores from the sensory evaluation showed that pasta fortified with PLP and red BPP can be a technological alternative to provide nutritionally enriched pasta.
意大利面在不同人群中非常受欢迎,是一种健康廉价的产品。因此,意大利面因其富含功能性成分而成为一个很有前途的对象。研究了红甜椒粉(BPP)和香菜叶粉(PLP)用于提高面食生物价值的可能性和可行性。研制了添加红BPP和PLP的意大利面条生产配方。研究了红甜椒粉和香菜叶粉对面食理化性质和烹饪性能的影响。意大利面的富集导致最佳烹饪时间、膨胀指数(SI)和吸水指数(WAI)降低。与对照样品相比,植物粉的添加显著降低了意大利面的亮度(P<0.05)。另一方面,蔬菜粉的添加对面食的总多酚含量和抗氧化活性有积极影响。在富含多酚的意大利面中,多酚含量增加了一倍(含10.0%BPP的意大利面),甚至增加了两倍(含10.0%PLP的意大利面的)。感官评估的得分表明,用PLP和红色BPP强化的意大利面可以成为提供营养丰富的意大利面的技术替代品。
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引用次数: 3
Application of the cell-free supernatant from Weissella viridescens to control Listeria monocytogenes Weissella viridescens无细胞上清液在控制单核细胞增多性李斯特菌中的应用
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-07-01 DOI: 10.17221/6/2022-cjfs
Chao Yang, Yingying Zhang, Tao Yu
Listeria monocytogenes can form biofilms on different food contact surfaces, increasing the risk of cross-contamination in food products. Weissella viridescens are Gram-positive bacteria and belong to lactic acid bacteria (LAB). The aim of this study was to investigate the antibacterial activity of cell-free culture supernatant (CFS) from W. viridescens to control L. monocytogenes biofilms as well as their growth on chilled pork. Preliminary results suggest that the antibacterial compounds in W. viridescens CFS may be proteinaceous in nature. The minimum inhibitory concentrations (MICs) of most L. monocytogenes strains tested was 30 mg mL–1. Biofilm formation of L. monocytogenes 10403S on stainless steel and polystyrene was significantly inhibited by the sub-inhibitory concentration of W. viridescens CFS (1/8MIC, 1/4MIC, and 1/2MIC). Our results also showed that W. viridescens CFS at concentrations higher than MIC (1MIC, 2MIC, and 4MIC) was effective in eradicating the mature biofilms of 10403S strain on various surfaces. Dip applications of W. viridescens CFS could inhibit the growth of L. monocytogenes on chilled pork. On the whole, W. viridescens CFS has the potential to control biofilms as a natural antibiofilm and as an antibacterial agent to inhibit the growth of L. monocytogenes on chilled pork.
单核细胞增多李斯特菌可以在不同的食物接触表面形成生物膜,增加食品中交叉污染的风险。Weissella viridescens是革兰氏阳性菌,属于乳酸菌(LAB)。本研究的目的是研究绿色念珠菌无细胞培养上清液(CFS)对单核细胞增多性李斯特菌生物膜的抗菌活性及其在冷冻猪肉上的生长。初步结果表明,W。病毒性慢性疲劳综合征可能是蛋白质性质的。大多数单核细胞增多性李斯特菌菌株的最低抑制浓度(MIC)为30 mg mL–1。L.的生物膜形成。亚抑制浓度的W。病毒性descens CFS(1/8MIC、1/4MIC和1/2MIC)。我们的研究结果还表明,在高于MIC(1MIC、2MIC和4MIC)的浓度下,绿色念珠菌能有效地根除10403S菌株在不同表面上的成熟生物膜。W的浸渍应用。绿脓杆菌CFS能抑制L的生长。冷冻猪肉上的单核细胞增多症。总的来说,绿色念珠菌CFS作为一种天然的抗菌膜和一种抑制L生长的抗菌剂,具有控制生物膜的潜力。冷冻猪肉上的单核细胞增多症。
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引用次数: 0
Evaluation of three-phase centrifugal separator machine (Tricanter) for olive oil extraction 三相离心分离机(Tricanter)萃取橄榄油的评价
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-23 DOI: 10.17221/278/2021-cjfs
Ashkan Shokrian, Q. Si, Peng Wang
One of the most important machines in the olive oil extraction line is the horizontal three-phase centrifugal separator machine or Tricanter. The purpose of this paper is to evaluate the machine designed on the basis of Tricanter and to evaluate the quality of extracted olive oil. For this purpose, four different olive cultivars from Gilan Province in Iran were used. In this research the rotational speed of the Tricanter machine was tested at three levels of 2 500, 3 000, and 3 500 rpm and the content of water added to olive paste was used at three levels of 10, 20, and 30% of the paste mass. Peroxide value (PV) and percentage of acidity were measured for oil extracted from all four olive cultivars. The results of the analysis of variance (ANOVA) test showed that rotational speed and the content of added water had an effect on the acidity and PV for all samples of olive cultivars. The measured values showed that the best speed for the Tricanter machine is 3 500 rpm. At this rotational speed, the peroxide and acidity values are lower than the standard values.
橄榄油提取线中最重要的机器之一是卧式三相离心分离机或Tricanter。本文的目的是评估基于Tricanter设计的机器,并评估提取橄榄油的质量。为此,使用了来自伊朗吉兰省的四种不同的橄榄品种。在这项研究中,Tricanter机器的转速分别在2500、3000和3500转/分三个水平上进行了测试,添加到橄榄酱中的水含量分别为橄榄酱质量的10%、20%和30%。测量了从所有四个橄榄品种中提取的油的过氧化值(PV)和酸度百分比。方差分析(ANOVA)检验结果表明,转速和加水量对所有橄榄品种的酸度和PV都有影响。测量值表明,Tricanter机器的最佳速度为3500rpm。在该转速下,过氧化物和酸度值低于标准值。
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引用次数: 0
Classification of hazelnuts according to their quality using deep learning algorithms 使用深度学习算法根据榛子的质量进行分类
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-20 DOI: 10.17221/21/2022-cjfs
Nizamettin Erbaş, Gokalp Cinarer, Kazım Kılıç
Hazelnut is a product with high nutritional and economic value. In maintaining the quality value of hazelnut, the classification process is of great importance. In the present day, the quality classification of hazelnuts and other crops is performed in general manually, and so it is difficult and costly. Performing this classification with modern agricultural techniques is much more important in terms of quality. This study was based on a model intended to detect hazelnut quality. The model is about the establishment of an artificial intelligence-based classification system that can detect the hidden defects of hazelnuts. In the developed model, the visuals used in the dataset are divided into training and test groups. In the model, hazelnuts are divided into 5 classes according to their quality using AlexNet architecture and modern deep learning (DL) techniques instead of traditional hazelnut classification methods. In this model developed based on artificial intelligence, a very good approach was presented with the accurate classification of 99%. Moreover, the values regarding precision and recall were also determined at 98.7% and 99.6%, respectively. This study is important in terms of becoming widespread information technology use and computer-assisted applications in the agricultural economics field such as product classification, quality, and control.
榛子是一种营养价值和经济价值都很高的产品。为了保持榛子的质量价值,分类过程至关重要。目前,榛子和其他作物的质量分类通常是手动进行的,因此这是困难和昂贵的。用现代农业技术进行这种分类在质量方面要重要得多。这项研究是基于一个旨在检测榛子质量的模型。该模型是关于建立一个基于人工智能的分类系统,可以检测榛子的隐藏缺陷。在开发的模型中,数据集中使用的视觉效果被分为训练组和测试组。在该模型中,使用AlexNet架构和现代深度学习(DL)技术,而不是传统的榛子分类方法,根据榛子的质量将其分为5类。在这个基于人工智能开发的模型中,提出了一种非常好的方法,准确分类率为99%。此外,准确度和召回率也分别为98.7%和99.6%。这项研究对于信息技术在农业经济领域(如产品分类、质量和控制)的广泛应用和计算机辅助应用具有重要意义。
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引用次数: 2
Packaging types as influencing seed quality in some soybean [Glycine max (L.) Merr.] varieties during storage period 几种大豆包装方式对种子品质的影响[j]稳定。]品种在储存期间
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-03 DOI: 10.17221/119/2021-cjfs
R. W. Arief, R. Asnawi, R. D. Tambunan, N. Wardani, D. Mustikawati, M. J. Mejaya
The post-harvest process at the storage stage is one of the most critical processes for maintaining soybean seed quality. The type of packaging and the type of material stored will affect its shelf life. One type of packaging has specific properties that are different from other types of packaging. Therefore, this study used several types of packaging materials and soybean varieties to determine changes in its quality during storage. The treatments were arranged in a 5 × 4 × 7 factorial experiment in a randomised block design (RBD) with three replications, consisting of totally 140 treatment combinations involving five different types of packaging materials. Water content, the weight of 100 grains, seedling dry weight, electrical conductivity (EC), and germination percentage were assessed monthly. The factorial analysis of variance (ANOVA) was used to compare water content, the weight of 100 grains, EC, germination, and seedling dry weight. A qualitative descriptive analysis method was employed for the temperature and relative humidity of the storage room. The results showed that Grobogan had the lowest germination percentage of 84.85% compared to the other varieties (86.97–90.82%). Plastic sack resulted in the most inferior seed quality (82.00% for plastic sack vs. 88.23–90.17% for the other containers). A more extended storage period of soybean seeds caused a decrease in soybean quality for all varieties and packaging materials. Up to 6 months of storage at room temperature, the quality of soybean seeds remained high, with the lowest germination of 80%.
收获后贮藏是保持大豆种子品质的关键工序之一。包装类型和储存材料类型会影响其保质期。一种包装具有不同于其他类型包装的特定属性。因此,本研究使用几种包装材料和大豆品种来确定其在储存过程中的质量变化。试验采用随机区组设计(RBD),采用5 × 4 × 7因子试验,3个重复,共140个处理组合,涉及5种不同的包装材料。水分含量、百粒重、幼苗干重、电导率和发芽率按月测定。采用因子方差分析(ANOVA)比较含水量、百粒重、EC、发芽率和幼苗干重。采用定性描述分析方法对仓库的温度和相对湿度进行了分析。结果表明,格罗博根的发芽率最低,为84.85%,高于其他品种(86.97 ~ 90.82%);塑料袋导致种子质量最差(塑料袋为82.00%,其他容器为88.23-90.17%)。大豆种子储存时间的延长导致所有品种和包装材料的大豆质量下降。在室温下保存6个月,大豆种子的质量保持较高,发芽率最低为80%。
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引用次数: 0
Traditional puffed fish cracker ('kemplang Palembang') by microwave-oven method: Physical properties and microstructure evaluation 微波炉法传统膨化炸鱼器(“kemplang Palembang”):物理性能和微观结构评估
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-02 DOI: 10.17221/52/2021-cjfs
Guttifera Guttifera, L. Rahmawati, Selly Ratna Sari, F. Pratama, T. Widowati
This research has investigated the physical properties (volume expansion, texture) and microstructure of 'kemplang Palembang', traditional fish puffed crackers from Indonesia which were puffed using the microwave-oven method. The microwave-oven method was designed by the factorial randomised block design (FRBD) which contains egg addition as factors (A1 – control; A2 – egg yolk; A3 – egg white and egg yolk) and moisture factors (B1 – 7.5 ± 1%; B2 – 13.5 ± 1%). The results revealed that the egg addition and moisture were significant (P < 0.05) to the volume expansion and texture. Meanwhile, the microstructure of kemplang Palembang was evidenced by 3D visual analysis using the scanning electron microscopy (SEM) technique. The microstructural analysis by SEM showed that the porosity caused a significant change in volume expansion and texture. The results suggest the feasibility of adding eggs and moisture 13.5 ± 1% (A1B2 treatment) for proper volume expansion (523%), texture [156.2 gram-force (gf)], and microstructure.
本研究考察了“kemplang Palembang”的物理性质(体积膨胀,质地)和微观结构,这是印度尼西亚传统的鱼膨化饼干,用微波炉膨化。微波炉法采用因子随机分组设计(FRBD),其中鸡蛋添加量为因素(A1 -对照;A2 -蛋黄;A3 -蛋清和蛋黄)和水分因子(B1 - 7.5±1%;b2 - 13.5±1%)。结果表明,鸡蛋添加量和水分对肉鸡的体积膨胀和质地有显著影响(P < 0.05)。同时,利用扫描电子显微镜(SEM)技术对坎普兰巨邦的微观结构进行了三维可视化分析。SEM显微组织分析表明,孔隙率使材料的体积膨胀和织构发生了显著变化。结果表明,添加鸡蛋和水分13.5±1% (A1B2处理)可获得适当的体积膨胀率(523%)、织构[156.2 g -force (gf)]和微观结构。
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引用次数: 3
Natural bioactive compounds of honey and their antimicrobial activity 蜂蜜的天然生物活性化合物及其抗菌活性
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-06-01 DOI: 10.17221/247/2021-cjfs
N. Feknous, M. Boumendjel
Honey is a complex and variable mixture that contains more than 180 biochemical compounds from various molecule families. This mixture is obtained after processing the nectar out of plant food sources at the level of the bee abdomen. The bioactive components found in this natural product are in charge of its antimicrobial properties. Honey is used for its antibacterial actions against Gram-positive (Gram+) and Gram-negative (Gram–) bacteria, its anti-fungal and antimycotic actions against moulds and yeasts, along with its protozoal and antiviral activities. This literature review outlines the natural antimicrobial potential of honey; it explains the factors responsible for this potential and spells out their mechanisms of action. Osmotic pressure, water activity, the acid content of honey, presence of bioactive compounds like hydrogen peroxide (H2O2), phenolic acids, flavonoids, methylglyoxal (MGO), defensin-1, lysozyme, volatile compounds as well as antibacterial products secreted from the lactic bacteria that are behind this antimicrobial activity. This potential basically depends on the biological activities of the initially harvested floral source, its geographical origin, season, storage conditions, honey age, health of bee colonies and suitable beekeeping practices.
蜂蜜是一种复杂多变的混合物,含有来自不同分子家族的180多种生物化学化合物。这种混合物是在蜜蜂腹部处理植物食物来源的花蜜后获得的。在这种天然产品中发现的生物活性成分负责其抗菌性能。蜂蜜因其对革兰氏阳性(Gram+)和革兰氏阴性(Gram-)细菌的抗菌作用、对霉菌和酵母的抗真菌和抗真菌作用以及原生动物和抗病毒活性而被使用。这篇文献综述概述了蜂蜜的天然抗菌潜力;它解释了造成这种潜力的因素,并阐明了它们的作用机制。渗透压、水活性、蜂蜜的酸含量、生物活性化合物的存在,如过氧化氢(H2O2)、酚酸、类黄酮、甲基乙二醛(MGO)、防御素-1、溶菌酶、挥发性化合物以及这种抗菌活性背后的乳酸菌分泌的抗菌产物。这种潜力基本上取决于最初收获的花源的生物活性、地理来源、季节、储存条件、蜂蜜年龄、蜂群的健康状况和适当的养蜂做法。
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引用次数: 11
Foam-mat convective drying of kiwiberry (Actinidia arguta) pulp 猕猴桃果肉泡沫垫对流干燥研究
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-05-31 DOI: 10.17221/263/2020-cjfs
Radosław Bogusz, A. Wiktor, M. Nowacka, Joanna Ćwintal, E. Gondek
The purpose of this work has been to determine the optimal conditions for the processing of kiwiberry fruit pulp based on the relevant thickness of the layer and air temperature, that will target the maximal reduction of drying time and minimal energy consumption. The research was designed to use the response surface methodology (RSM). The effects of independent variables (factors) such as the thickness of the layer (4, 8, and 12 mm) and the temperature of the drying air (50, 60, and 70°C) were studied by the means of the response variables. In this study, the drying kinetics was evaluated based on the drying time, drying rate, and effective water diffusion coefficient (Deff). Based on the simplified Fick's second law of diffusion, Deff was determined, while the relative water content [moisture ratio (MR)] was predicted using empirical models. It was found that as the temperature of the drying process increased, the process time was reduced, and the drying process intensified, which was manifested by the increment of the drying rate as well as the effective moisture diffusivity (varied from 5.58 × 10–10 m2 s–1 to 27.2 × 10–10 m2 s–1). The mathematical model, developed using the RSM, showed a good fit for the data obtained in the study, which allowed to predict the response of the effective water Deff of the foamed kiwiberry fruit pulp with R2 > 0.97.
本研究的目的是根据猕猴桃果肉的相关层厚和空气温度来确定猕猴桃果肉的最佳加工条件,以最大限度地缩短干燥时间和最小的能耗为目标。本研究采用响应面法(RSM)。通过响应变量研究了层厚(4、8、12 mm)和干燥空气温度(50、60、70℃)等自变量(因子)的影响。在这项研究中,根据干燥时间、干燥速率和有效水扩散系数(Deff)来评估干燥动力学。基于简化的菲克第二扩散定律确定了Deff,并利用经验模型预测了相对含水量[含水率(MR)]。研究发现,随着干燥温度的升高,干燥时间缩短,干燥过程加剧,表现为干燥速率和有效水分扩散率的增加(从5.58 × 10-10 m2 s-1增加到27.2 × 10-10 m2 s-1)。利用RSM建立的数学模型与实验数据拟合较好,可以预测发泡猕猴桃果肉的有效含水率响应R2为> 0.97。
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引用次数: 0
Utilisation of waste Agaricus bisporus and Torreya grandis as potential natural additive coating in Agaricus bisporus preservation 废双孢蘑菇和大香榧作为天然添加剂包衣在双孢蘑菇保鲜中的应用
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-05-31 DOI: 10.17221/91/2020-cjfs
Haining Zhuang, Mo Yang, T. Feng, Wenfeng Ding, Ling Chen, Yongjin Qiao, Da Chen, Jin-Song Zhang, W. Jia
Natural additives obtained from vegetable and fruit wastes can potentially be used as coatings to enhance the shelf life of button mushrooms (Agaricus bisporus). The present study was therefore conducted to assess the effects of natural additive coatings A. bisporus polysaccharides (ABP) and Torreya grandis essential oils (TEO) on the post-harvest quality of button mushrooms up to 16 days under cold storage (4 °C). Different concentrations for coatings were used including control (no additives), 500, 1 000, 5 000 ppm or a combination of ABP and TEO. Changes in weight loss, firmness, polyphenol oxidase (PPO) activity and microbial biomass were investigated as well as the sensory evaluation was carried out. Results show a significant decrease in the weight loss rate (decreased up to 2.34%), down regulated the PPO activities (reduced up to 30.15%) and maintained tissue firmness in ABP/TEO treatments compared to the control, whereas TEO inhibited especially the growth of microorganisms. Natural additive coatings can better protect the inner mushroom colour and flavour compared to the control.
从蔬菜和水果废料中获得的天然添加剂有可能用作涂层,以延长蘑菇(双孢蘑菇)的保质期。因此,本研究旨在评估天然添加剂涂层双孢蘑菇多糖(ABP)和香榧精油(TEO)对冷藏(4°C)下长达16天的蘑菇采后质量的影响。使用不同浓度的涂层,包括对照(无添加剂)、500、1000、5000ppm或ABP和TEO的组合。研究了失重、硬度、多酚氧化酶(PPO)活性和微生物生物量的变化,并进行了感官评价。结果显示,与对照组相比,ABP/TEO处理显著降低了体重减轻率(降低了2.34%),下调了PPO活性(降低了30.15%),并保持了组织硬度,而TEO尤其抑制了微生物的生长。与对照相比,天然添加剂涂层可以更好地保护蘑菇内部的颜色和风味。
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引用次数: 0
Transcriptomic analysis identifies differentially expressed genes in purple tender shoots and green mature leaves of Zijuan tea 紫卷茶紫色嫩芽和绿色成熟叶差异表达基因的转录组学分析
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-05-17 DOI: 10.17221/158/2020-cjfs
Bin Jiang, Lijiao Chen, Shuang-mei Duan, Bo Nian, Kunyi Liu, Yan Ma, Ming Zhao
Purple shoots of tea germplasm Zijuan (Camellia sinensis var. asssamica) have high anthocyanin content and gradually turn green with continued growth and development. To uncover the mechanism of this colour change, we performed transcriptome analysis to identify differentially expressed genes (DEGs). A total of 18 328 DEGs were identified in purple tender shoots of Zijuan (ZJP) compared with mature green leaves of Zijuan (ZJG). These included 85 DEGs associated with phenylpropanoid biosynthesis, 30 DEGs associated with flavonoid biosynthesis, and 3 DEGs involved in flavone and flavonol biosynthesis. We detected higher expression in ZJP than in ZJG of genes encoding the following key enzymes regulating anthocyanin biosynthesis and their corresponding metabolites: chalcone synthase (CHS), anthocyanidin synthase (ANS), anthocyanidin reductase (ANR), adenosine triphosphate (ATP)-binding cassette (ABC) transporters, bifunctional 3-dehydroquinate dehydratase (DHQ), chorismate mutase (CM), cinnamoyl-CoA reductase 1 (CCR), and cinnamyl alcohol dehydrogenase (CAD). In addition, upregulated expression of carbohydrate metabolism, glycolysis pathway, sucrose metabolism, and pyruvate metabolism suggested that glycolysis and upregulation of the sucrose synthase (SUS) gene may provide more intermediates as substrates for promoting anthocyanin accumulation in ZJP. Moreover, DEGs involved in anthocyanin biosynthesis, including phenylalanine ammonialyase (PAL), cinnamate acid 4-hydroxylase (C4H), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS), ANR, ANS, CHS, and flavonoid 3',5'-hydroxylase (F3'5'H) were significantly (P < 0.01) correlated with the contents of anthocyanin components such as cyanidin (Cy), delphinidin (Dp), peonidin (Pn), petunidin (Pt), and malvidin (Mv). The above results suggested that these upregulated DEGs may contribute to anthocyanin accumulation in ZJP and may play important roles in the colour changes in Zijuan tea. This research provided a foundation for clarifying the mechanisms underlying colour changes in Zijuan tea.
紫鹃紫梢花青素含量高,随着生长发育逐渐变绿。为了揭示这种颜色变化的机制,我们进行了转录组分析,以鉴定差异表达基因(DEG)。紫鹃(ZJP)紫色嫩芽与紫鹃成熟绿叶相比,共鉴定出18328个DEG。其中包括85个与苯丙素生物合成相关的DEG,30个与类黄酮生物合成相关的DeG,以及3个与黄酮和黄酮醇生物合成有关的DEG。我们检测到编码以下调控花青素生物合成的关键酶及其相应代谢产物的基因在ZJP中的表达高于在ZJG中的表达:查尔酮合酶(CHS)、花青素合酶(ANS)、花青素还原酶(ANR)、三磷酸腺苷(ATP)结合盒(ABC)转运蛋白、双功能3-脱氢奎尼酸脱水酶(DHQ)、,肉桂酰辅酶A还原酶1(CCR)和肉桂醇脱氢酶(CAD)。此外,糖代谢、糖酵解途径、蔗糖代谢和丙酮酸代谢的上调表达表明,糖酵解和蔗糖合成酶(SUS)基因的上调可能为促进ZJP中花青素积累提供更多的中间体作为底物。此外,参与花青素生物合成的DEG,包括苯丙氨酸解氨酶(PAL)、肉桂酸4-羟化酶(C4H)、查尔酮异构酶(CHI)、黄烷酮3-羟化酶(F3H)、黄酮醇合成酶(FLS)、ANR、ANS、CHS和类黄酮3',5'-羟化酶(F3’5'H),与花青素(Cy)等花青素成分的含量显著相关(P<0.01),飞燕草苷(Dp)、飞燕草素(Pn)、小飞燕草蛋白(Pt)和malvidin(Mv)。上述结果表明,这些上调的DEG可能有助于ZJP中花青素的积累,并可能在紫卷茶的颜色变化中发挥重要作用。本研究为阐明紫卷茶颜色变化的机理提供了基础。
{"title":"Transcriptomic analysis identifies differentially expressed genes in purple tender shoots and green mature leaves of Zijuan tea","authors":"Bin Jiang, Lijiao Chen, Shuang-mei Duan, Bo Nian, Kunyi Liu, Yan Ma, Ming Zhao","doi":"10.17221/158/2020-cjfs","DOIUrl":"https://doi.org/10.17221/158/2020-cjfs","url":null,"abstract":"Purple shoots of tea germplasm Zijuan (Camellia sinensis var. asssamica) have high anthocyanin content and gradually turn green with continued growth and development. To uncover the mechanism of this colour change, we performed transcriptome analysis to identify differentially expressed genes (DEGs). A total of 18 328 DEGs were identified in purple tender shoots of Zijuan (ZJP) compared with mature green leaves of Zijuan (ZJG). These included 85 DEGs associated with phenylpropanoid biosynthesis, 30 DEGs associated with flavonoid biosynthesis, and 3 DEGs involved in flavone and flavonol biosynthesis. We detected higher expression in ZJP than in ZJG of genes encoding the following key enzymes regulating anthocyanin biosynthesis and their corresponding metabolites: chalcone synthase (CHS), anthocyanidin synthase (ANS), anthocyanidin reductase (ANR), adenosine triphosphate (ATP)-binding cassette (ABC) transporters, bifunctional 3-dehydroquinate dehydratase (DHQ), chorismate mutase (CM), cinnamoyl-CoA reductase 1 (CCR), and cinnamyl alcohol dehydrogenase (CAD). In addition, upregulated expression of carbohydrate metabolism, glycolysis pathway, sucrose metabolism, and pyruvate metabolism suggested that glycolysis and upregulation of the sucrose synthase (SUS) gene may provide more intermediates as substrates for promoting anthocyanin accumulation in ZJP. Moreover, DEGs involved in anthocyanin biosynthesis, including phenylalanine ammonialyase (PAL), cinnamate acid 4-hydroxylase (C4H), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS), ANR, ANS, CHS, and flavonoid 3',5'-hydroxylase (F3'5'H) were significantly (P < 0.01) correlated with the contents of anthocyanin components such as cyanidin (Cy), delphinidin (Dp), peonidin (Pn), petunidin (Pt), and malvidin (Mv). The above results suggested that these upregulated DEGs may contribute to anthocyanin accumulation in ZJP and may play important roles in the colour changes in Zijuan tea. This research provided a foundation for clarifying the mechanisms underlying colour changes in Zijuan tea.","PeriodicalId":10882,"journal":{"name":"Czech Journal of Food Sciences","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45190224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Czech Journal of Food Sciences
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