The aim of the present study was to compare carcass characteristics and meat quality of fast- (Ross 308), medium- (Hubbard JA757) and slow-growing (ISA Dual) chickens (n = 1 980). When the chickens reached an average live weight of 2 kg, 40 birds (males to females, 1 : 1) of each genotype were randomly selected and slaughtered for carcass analysis. Pectoralis major (PM) muscle samples were taken to determine the physical and chemical parameters of the meat quality. The fast- and medium-growing genotypes had higher (+3.24% and +3.84%, respectively) dressing out percentages than the slow-growing chickens. As expected, the breast percentage significantly decreased in the order of fast growth > medium growth > slow growth. The abdominal fat percentage was the lowest in Ross 308 chickens, but the abdominal fat percentage in JA757 and ISA Dual chickens did not differ. For edible organs, fast- and medium-growing chickens had heavier hearts and livers than slow-growing chickens. In contrast, ISA Dual chickens showed heavier gizzards. Genotype dramatically affected the chemical composition of PM muscle. The ISA Dual chickens exhibited a significantly higher proportion of dry matter and protein and lower ether extract and cholesterol content than Ross 308 chickens, with intermediate values for JA757 chickens. The ISA Dual group had a lower ash content than Ross 308 and JA757 groups. In terms of physical parameters, the ISA Dual chickens had higher lightness and yellowness and lower redness, pH 24, and cooking loss than those of the other genotypes. Concerning all genotypes, the shear force values increased from fast- to slow-growing chickens. In conclusion, this study found a difference between the genotypes. Although the slow-growing chickens showed the lowest dressing out and breast percentages compared with the other genotypes, the breast meat of the slow-growing chickens had more favourable nutritional properties.
{"title":"Carcass characteristics and breast meat quality in fast-, medium- and slow-growing chickens","authors":"Jaroslav Valenta, D. Chodová, E. Tůmová, M. Ketta","doi":"10.17221/91/2022-cjas","DOIUrl":"https://doi.org/10.17221/91/2022-cjas","url":null,"abstract":"The aim of the present study was to compare carcass characteristics and meat quality of fast- (Ross 308), medium- (Hubbard JA757) and slow-growing (ISA Dual) chickens (n = 1 980). When the chickens reached an average live weight of 2 kg, 40 birds (males to females, 1 : 1) of each genotype were randomly selected and slaughtered for carcass analysis. Pectoralis major (PM) muscle samples were taken to determine the physical and chemical parameters of the meat quality. The fast- and medium-growing genotypes had higher (+3.24% and +3.84%, respectively) dressing out percentages than the slow-growing chickens. As expected, the breast percentage significantly decreased in the order of fast growth > medium growth > slow growth. The abdominal fat percentage was the lowest in Ross 308 chickens, but the abdominal fat percentage in JA757 and ISA Dual chickens did not differ. For edible organs, fast- and medium-growing chickens had heavier hearts and livers than slow-growing chickens. In contrast, ISA Dual chickens showed heavier gizzards. Genotype dramatically affected the chemical composition of PM muscle. The ISA Dual chickens exhibited a significantly higher proportion of dry matter and protein and lower ether extract and cholesterol content than Ross 308 chickens, with intermediate values for JA757 chickens. The ISA Dual group had a lower ash content than Ross 308 and JA757 groups. In terms of physical parameters, the ISA Dual chickens had higher lightness and yellowness and lower redness, pH 24, and cooking loss than those of the other genotypes. Concerning all genotypes, the shear force values increased from fast- to slow-growing chickens. In conclusion, this study found a difference between the genotypes. Although the slow-growing chickens showed the lowest dressing out and breast percentages compared with the other genotypes, the breast meat of the slow-growing chickens had more favourable nutritional properties.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42657477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the relationships of temperature humidity index (THI) with milk yield and some physiological responses in dairy cattle were investigated. Our goal in the meta-analysis was to find the parameter(s) primarily affected under heat stress. A total of 16 studies with the temperature humidity index value higher than 72, which is an important factor in determining the effect of heat stress, were included in the meta-analysis. The variables of interest in the meta-analysis included: milk yield (kg/day), respiratory rate (breaths/min), rectal temperature (°C). In addition to the meta-analysis, principal component analysis (PCA) was also performed. In the meta-analysis, high variation or heterogeneity (I2 > 99%) was determined between the results of the studies. This may depend on many factors (climate, region, number of samples and management etc.). Heterogeneity is desirable in the meta-analysis, because it provides accurate and reliable interpretations of the variances of parameters. Due to high heterogeneity, the results of the studies were combined according to the mixed model. According to the mixed model and PCA results, a linear relationship was determined between the temperature humidity index and these physiological parameters. According to the meta-analysis, at THI > 72, the mean effect size of milk yield was 50%, and the effect sizes of respiratory rate and rectal temperature were approximately 65% and 38%. All three parameters have a significant effect under heat stress (P < 0.000 1). As a result, there is a linear relationship between temperature humidity index, milk yield and physiological parameters. According to the other characteristics, the respiratory rate was determined as the primary response parameter in parallel with the increase in temperature humidity index.
{"title":"A meta-analysis of heat stress in dairy cattle: The increase in temperature humidity index affects both milk yield and some physiological parameters","authors":"Ersin Kulaz, G. Ser","doi":"10.17221/1/2022-cjas","DOIUrl":"https://doi.org/10.17221/1/2022-cjas","url":null,"abstract":"In this study, the relationships of temperature humidity index (THI) with milk yield and some physiological responses in dairy cattle were investigated. Our goal in the meta-analysis was to find the parameter(s) primarily affected under heat stress. A total of 16 studies with the temperature humidity index value higher than 72, which is an important factor in determining the effect of heat stress, were included in the meta-analysis. The variables of interest in the meta-analysis included: milk yield (kg/day), respiratory rate (breaths/min), rectal temperature (°C). In addition to the meta-analysis, principal component analysis (PCA) was also performed. In the meta-analysis, high variation or heterogeneity (I2 > 99%) was determined between the results of the studies. This may depend on many factors (climate, region, number of samples and management etc.). Heterogeneity is desirable in the meta-analysis, because it provides accurate and reliable interpretations of the variances of parameters. Due to high heterogeneity, the results of the studies were combined according to the mixed model. According to the mixed model and PCA results, a linear relationship was determined between the temperature humidity index and these physiological parameters. According to the meta-analysis, at THI > 72, the mean effect size of milk yield was 50%, and the effect sizes of respiratory rate and rectal temperature were approximately 65% and 38%. All three parameters have a significant effect under heat stress (P < 0.000 1). As a result, there is a linear relationship between temperature humidity index, milk yield and physiological parameters. According to the other characteristics, the respiratory rate was determined as the primary response parameter in parallel with the increase in temperature humidity index.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47572695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yutong Sun, Guohong Wang, Xinbo Zhou, Yuchen Sun, Qingquan Ma
Milk production determines health status and optimal growth of suckling neonates. Recent studies revealed that the secretion of leucine into sow milk from the mammary gland is significantly lower than the amount taken up by the mammary gland from the blood, suggesting the potential and fundamental role in udder metabolism. However, little is known about the underlying mechanism. In this study, porcine mammary epithelial cells (PMECs) were employed to investigate the effects of leucine on protein synthesis and mammalian target of rapamycin (mTOR) signalling. Results showed that leucine supplementation significantly increased the proliferation of PMECs in a dose-dependent manner from 0 to 1 mmol/l. Secretions of four casein subunits were significantly increased by addition of leucine. The phosphorylation of proteins related to mTOR signalling, including mTOR, eukaryotic translation initiation factor 4E-binding protein, and p70 ribosomal protein S6 kinase, were accordingly upregulated by leucine supplementation. Casein secretion was not affected by leucine treatment when mTOR signalling was inhibited by rapamycin. This suggests that leucine stimulates protein synthesis through the mTOR signalling pathway. These findings facilitate the utilization of dietary leucine supplementation to improve milk production in lactating sows.
{"title":"L-Leucine stimulates proliferation and casein secretion in porcine mammary epithelial cells through mTOR-p70S6K-4EBP1 signalling pathway","authors":"Yutong Sun, Guohong Wang, Xinbo Zhou, Yuchen Sun, Qingquan Ma","doi":"10.17221/121/2021-cjas","DOIUrl":"https://doi.org/10.17221/121/2021-cjas","url":null,"abstract":"Milk production determines health status and optimal growth of suckling neonates. Recent studies revealed that the secretion of leucine into sow milk from the mammary gland is significantly lower than the amount taken up by the mammary gland from the blood, suggesting the potential and fundamental role in udder metabolism. However, little is known about the underlying mechanism. In this study, porcine mammary epithelial cells (PMECs) were employed to investigate the effects of leucine on protein synthesis and mammalian target of rapamycin (mTOR) signalling. Results showed that leucine supplementation significantly increased the proliferation of PMECs in a dose-dependent manner from 0 to 1 mmol/l. Secretions of four casein subunits were significantly increased by addition of leucine. The phosphorylation of proteins related to mTOR signalling, including mTOR, eukaryotic translation initiation factor 4E-binding protein, and p70 ribosomal protein S6 kinase, were accordingly upregulated by leucine supplementation. Casein secretion was not affected by leucine treatment when mTOR signalling was inhibited by rapamycin. This suggests that leucine stimulates protein synthesis through the mTOR signalling pathway. These findings facilitate the utilization of dietary leucine supplementation to improve milk production in lactating sows.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46674469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junjie Zhang, Zhiyuan Yue, Yuchen Sun, Zhishen Wang, Yiming Zhang, Libin Huang, J-M Tu, B. Shi, A. Shan, Q. Ma
We investigated the effects of sweet potato vine silage (SPVS) supplementation on the growth performance, apparent digestibility and gut health of finishing pigs. 180 Bali Black pigs (Berkshire × Licha Black, with body weight of 74.54 ± 3.32 kg) were assigned to three groups: basal diet (Ctrl), Ctrl supplemented with 2.5% SPVS (Lspvs) and 5.0% SPVS (Hspvs). Animals were slaughtered after nine weeks of feeding. The results indicated that dietary SPVS supplementation improved average daily food intake and average daily gain. However, SPVS treatment decreased the apparent digestibility. Activities of antioxidant enzymes including total superoxide dismutase, total antioxidant capacity, glutathione peroxidase and catalase in the intestines of the Lspvs group were markedly upregulated. Concentrations of IL-1β and IL-6 were decreased and secretory immunoglobulin A was increased in Lspvs group. A significant increase (P < 0.05) of ileum diamine oxidase in Lspvs group was observed. The ileum villus height/crypt depth in the Hspvs group was significantly reduced. The ratio of Firmicutes to Bacteroidetes in the caecum contents of pigs was reduced, and the abundance of Lactobacillus was significantly increased. Specifically, Hspvs treatment markedly reduced the abundances of Proteobacteria. Collectively, these results suggest that dietary supplementation with SPVS is capable of improving growth performance, immune function and intestinal health by promoting the balance of the intestinal microbiota.
{"title":"Effects of sweet potato vine silage supplementation on growth performance, nutrient digestibility, and intestinal health in finishing pigs","authors":"Junjie Zhang, Zhiyuan Yue, Yuchen Sun, Zhishen Wang, Yiming Zhang, Libin Huang, J-M Tu, B. Shi, A. Shan, Q. Ma","doi":"10.17221/209/2021-cjas","DOIUrl":"https://doi.org/10.17221/209/2021-cjas","url":null,"abstract":"We investigated the effects of sweet potato vine silage (SPVS) supplementation on the growth performance, apparent digestibility and gut health of finishing pigs. 180 Bali Black pigs (Berkshire × Licha Black, with body weight of 74.54 ± 3.32 kg) were assigned to three groups: basal diet (Ctrl), Ctrl supplemented with 2.5% SPVS (Lspvs) and 5.0% SPVS (Hspvs). Animals were slaughtered after nine weeks of feeding. The results indicated that dietary SPVS supplementation improved average daily food intake and average daily gain. However, SPVS treatment decreased the apparent digestibility. Activities of antioxidant enzymes including total superoxide dismutase, total antioxidant capacity, glutathione peroxidase and catalase in the intestines of the Lspvs group were markedly upregulated. Concentrations of IL-1β and IL-6 were decreased and secretory immunoglobulin A was increased in Lspvs group. A significant increase (P < 0.05) of ileum diamine oxidase in Lspvs group was observed. The ileum villus height/crypt depth in the Hspvs group was significantly reduced. The ratio of Firmicutes to Bacteroidetes in the caecum contents of pigs was reduced, and the abundance of Lactobacillus was significantly increased. Specifically, Hspvs treatment markedly reduced the abundances of Proteobacteria. Collectively, these results suggest that dietary supplementation with SPVS is capable of improving growth performance, immune function and intestinal health by promoting the balance of the intestinal microbiota.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42793623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Xin, Ju Guo, B. Yu, Hao Li, Y. Gu, Qiaoxian Gao, Juan Zhang
The present study investigated the effect of different dietary levels (0%, 0.25%, 0.5%, 1%, 2%) of Sophora alopecuroides (S. alopecuroides) on intramuscular fat (IMF) content in Ningxia Tan sheep. Candidate genes affecting IMF deposition were screened by comparing the RNA-Seq profiles of test (2% S. alopecuroides) and control (no S. alopecuroides) groups. The results showed that the intramuscular fat content of the diet supplemented with S. alopecuroides was significantly lower than that of the control group, and the intramuscular fat content decreased significantly with the increase of the dietary content of S. alopecuroides (P < 0.05). The highest IMF content of the control group (group A) was 4.21%, which was significantly higher than that of the other four experimental groups (B, C, D, E) (P < 0.05). Moreover, 66 genes were significantly upregulated and 202 genes were significantly downregulated in the test group compared to the control. Gene Ontology functional annotation revealed that the differentially expressed genes were closely related to plasma lipoprotein particle assembly, plasma lipoprotein particle organization, protein-lipid complex subunit organization, and other biological processes. KEGG pathway analysis showed that the differentially expressed genes belonged to 147 metabolic pathways. Finally, four differential genes which may be related to IMF deposition were screened out in PPAR signalling pathway, fatty acid biosynthesis, fatty acid degradation, and fatty acid metabolism. The quantitative analysis of four differential genes including ACSL3, PLIN2, ABCA1 and ANGPTL4 was carried out by RT-qPCR method, and the results were basically consistent with those of RNA-seq. Among them, the quantitative results of PLIN2 gene were slightly different from the sequencing results, but the difference was not significant, and they may be false positive. This study lays the foundation for understanding the molecular mechanism regulating mutton quality, and provides a theoretical basis for the study of S. alopecuroides as a feed additive to improve mutton meat quality and impact the gene function.
{"title":"Effect of dietary Sophora alopecuroides supplementation on differential expression of intramuscular fat-related genes of Ningxia Tan sheep as determined by transcriptome sequencing","authors":"G. Xin, Ju Guo, B. Yu, Hao Li, Y. Gu, Qiaoxian Gao, Juan Zhang","doi":"10.17221/127/2021-cjas","DOIUrl":"https://doi.org/10.17221/127/2021-cjas","url":null,"abstract":"The present study investigated the effect of different dietary levels (0%, 0.25%, 0.5%, 1%, 2%) of Sophora alopecuroides (S. alopecuroides) on intramuscular fat (IMF) content in Ningxia Tan sheep. Candidate genes affecting IMF deposition were screened by comparing the RNA-Seq profiles of test (2% S. alopecuroides) and control (no S. alopecuroides) groups. The results showed that the intramuscular fat content of the diet supplemented with S. alopecuroides was significantly lower than that of the control group, and the intramuscular fat content decreased significantly with the increase of the dietary content of S. alopecuroides (P < 0.05). The highest IMF content of the control group (group A) was 4.21%, which was significantly higher than that of the other four experimental groups (B, C, D, E) (P < 0.05). Moreover, 66 genes were significantly upregulated and 202 genes were significantly downregulated in the test group compared to the control. Gene Ontology functional annotation revealed that the differentially expressed genes were closely related to plasma lipoprotein particle assembly, plasma lipoprotein particle organization, protein-lipid complex subunit organization, and other biological processes. KEGG pathway analysis showed that the differentially expressed genes belonged to 147 metabolic pathways. Finally, four differential genes which may be related to IMF deposition were screened out in PPAR signalling pathway, fatty acid biosynthesis, fatty acid degradation, and fatty acid metabolism. The quantitative analysis of four differential genes including ACSL3, PLIN2, ABCA1 and ANGPTL4 was carried out by RT-qPCR method, and the results were basically consistent with those of RNA-seq. Among them, the quantitative results of PLIN2 gene were slightly different from the sequencing results, but the difference was not significant, and they may be false positive. This study lays the foundation for understanding the molecular mechanism regulating mutton quality, and provides a theoretical basis for the study of S. alopecuroides as a feed additive to improve mutton meat quality and impact the gene function.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42626617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabel Cristina Acosta-Balcazar, Jorge Quiroz-Valiente, Lorenzo Granados-Zurita, E. Aranda-Ibáñez, E. Hernández-Nataren, J. Rincón-Ramírez, L. D. Granados-Rivera
Milk fat from bovine milk contains fatty acids that may have favourable properties for human health, for example, conjugated linoleic acid (CLA) has nutraceutical activity. This research aimed to know the effects of genotype, days of lactation and climatic factors on the fatty acids (FA) profile of milk and particularly the content of CLA in milk fat. Seventeen first-calving milking cows in early lactation were used for the assessment of milk; 12 were Gyr and five were F1 (Holstein/Gyr) crosses. Sampling was carried out every 15 days, from the beginning to the end of lactation (300 days). Fatty acids were analyzed employing gas chromatography. The genotype did not influence the content of the fatty acid groups: saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and CLA. The highest MUFA and PUFA contents were recorded at 100 days of lactation (32.334 and 3.553 g/100 g of FA, respectively), while SFA and CLA had their highest production at 200 days of lactation (63.238 and 1.378 g/100 g of FA, respectively). Regarding the climate, the highest temperature caused a decrease in the CLA content, because temperatures above 30 °C caused a decrease in the grazing time.
来自牛乳的乳脂含有可能对人体健康有益的脂肪酸，例如，共轭亚油酸(CLA)具有营养保健活性。本研究旨在了解基因型、哺乳天数和气候因素对乳汁脂肪酸(FA)分布，特别是乳脂中CLA含量的影响。选取17头初产乳奶牛进行泌乳评价;Gyr杂交12个，F1 (Holstein/Gyr)杂交5个。从泌乳期开始至泌乳期结束(300 d)，每隔15天采样一次。采用气相色谱法对脂肪酸进行分析。基因型对饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)、多不饱和脂肪酸(PUFA)和多不饱和脂肪酸(CLA)的含量没有影响。泌乳100天MUFA和PUFA含量最高，分别为32.334 g/100 g FA和3.553 g/100 g FA，泌乳200天SFA和CLA含量最高，分别为63.238 g/100 g FA和1.378 g/100 g FA。气候方面，最高温度导致CLA含量下降，因为温度高于30℃导致放牧时间减少。
{"title":"Effect of genotype, lactation and climatic factors on fatty acid profile of bovine milk","authors":"Isabel Cristina Acosta-Balcazar, Jorge Quiroz-Valiente, Lorenzo Granados-Zurita, E. Aranda-Ibáñez, E. Hernández-Nataren, J. Rincón-Ramírez, L. D. Granados-Rivera","doi":"10.17221/32/2022-cjas","DOIUrl":"https://doi.org/10.17221/32/2022-cjas","url":null,"abstract":"Milk fat from bovine milk contains fatty acids that may have favourable properties for human health, for example, conjugated linoleic acid (CLA) has nutraceutical activity. This research aimed to know the effects of genotype, days of lactation and climatic factors on the fatty acids (FA) profile of milk and particularly the content of CLA in milk fat. Seventeen first-calving milking cows in early lactation were used for the assessment of milk; 12 were Gyr and five were F1 (Holstein/Gyr) crosses. Sampling was carried out every 15 days, from the beginning to the end of lactation (300 days). Fatty acids were analyzed employing gas chromatography. The genotype did not influence the content of the fatty acid groups: saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and CLA. The highest MUFA and PUFA contents were recorded at 100 days of lactation (32.334 and 3.553 g/100 g of FA, respectively), while SFA and CLA had their highest production at 200 days of lactation (63.238 and 1.378 g/100 g of FA, respectively). Regarding the climate, the highest temperature caused a decrease in the CLA content, because temperatures above 30 °C caused a decrease in the grazing time.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48243816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Loučka, Václav Jambor, J. Nedělník, J. Lang, P. Homolka, F. Jančík, V. Koukolová, Petra Kubelková, Y. Tyrolová, A. Výborná
The aim of this study is to compare the differences between four maize hybrids in terms of nutrient determination by portable near-infrared reflectance spectrometer (pNIRS) and chemical analysis; each of the hybrids was grown in the same locality from 2018 to 2020. The topic relates to the variability of the feed value of maize being an important feedstuff in livestock nutrition. The nutritional values determined by pNIRS in comparison with the chemical analysis were higher (P < 0.001) in starch and ash content but lower in dry matter, neutral detergent fibre and crude protein (CP) content. The digestibility levels of neutral detergent fibre and the net energy of lactation as well as the potential milk production per hectare in relation to each tonne of dry matter were also lower. According to this result, it would be necessary to calibrate all tested indicators for a given spectrometer. However, the pNIRS results are useful for evaluating nutrient variability; the standard deviation of the values found in pNIRS was mostly lower than that determined chemically. The pNIRS results are also useful for making practical adjustments to the total mixed rations when calculated from actual chemical analysis if the correlation between the two methods is used; the correlation between the pNIRS and chemical results was found to be significant (P < 0.05) in terms of all the indicators.
{"title":"Differences between chemical analysis and portable near-infrared reflectance spectrometry in maize hybrids","authors":"R. Loučka, Václav Jambor, J. Nedělník, J. Lang, P. Homolka, F. Jančík, V. Koukolová, Petra Kubelková, Y. Tyrolová, A. Výborná","doi":"10.17221/23/2022-cjas","DOIUrl":"https://doi.org/10.17221/23/2022-cjas","url":null,"abstract":"The aim of this study is to compare the differences between four maize hybrids in terms of nutrient determination by portable near-infrared reflectance spectrometer (pNIRS) and chemical analysis; each of the hybrids was grown in the same locality from 2018 to 2020. The topic relates to the variability of the feed value of maize being an important feedstuff in livestock nutrition. The nutritional values determined by pNIRS in comparison with the chemical analysis were higher (P < 0.001) in starch and ash content but lower in dry matter, neutral detergent fibre and crude protein (CP) content. The digestibility levels of neutral detergent fibre and the net energy of lactation as well as the potential milk production per hectare in relation to each tonne of dry matter were also lower. According to this result, it would be necessary to calibrate all tested indicators for a given spectrometer. However, the pNIRS results are useful for evaluating nutrient variability; the standard deviation of the values found in pNIRS was mostly lower than that determined chemically. The pNIRS results are also useful for making practical adjustments to the total mixed rations when calculated from actual chemical analysis if the correlation between the two methods is used; the correlation between the pNIRS and chemical results was found to be significant (P < 0.05) in terms of all the indicators.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45768540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The experiment aimed to determine the effect of 50% (LS50) or 100% (LS100) substitution of lupin protein (variety Zulika) for soybean protein in feed mixtures intended for the nutrition of broiler chickens, on muscular fat quality (composition of fatty acids). There were established three groups of chickens for fattening with 80 individuals each (control group C and experimental groups). After the 34-day fattening period chicken breast and thigh muscles were analysed to find out the fatty acid composition. Lupin protein-based diets had a positive effect on the muscle quality of fattened chickens due to changes in fatty acid composition, compared to soya protein-based diets. The feeding of lupin-based diets to broiler chickens resulted in the reduction of saturated fatty acids (P ≤ 0.05) by 14% in LS50 group and 17% in LS100 group and increase of unsaturated fatty acids (P ≤ 0.05) by 58% in LS50 group and 90% in LS100 group in muscle fat. The results clearly confirm that lupin-based diet increases the dietary value of chicken meat as one of the most important protein sources in human nutrition.
{"title":"Effect of partial and complete substitution of lupin meal for soybean meal in diets on changes in fatty acid composition of muscle fat in broiler chickens","authors":"M. Kutlvašr, E. Strakova, P. Suchý, L. Všetičková","doi":"10.17221/56/2022-cjas","DOIUrl":"https://doi.org/10.17221/56/2022-cjas","url":null,"abstract":"The experiment aimed to determine the effect of 50% (LS50) or 100% (LS100) substitution of lupin protein (variety Zulika) for soybean protein in feed mixtures intended for the nutrition of broiler chickens, on muscular fat quality (composition of fatty acids). There were established three groups of chickens for fattening with 80 individuals each (control group C and experimental groups). After the 34-day fattening period chicken breast and thigh muscles were analysed to find out the fatty acid composition. Lupin protein-based diets had a positive effect on the muscle quality of fattened chickens due to changes in fatty acid composition, compared to soya protein-based diets. The feeding of lupin-based diets to broiler chickens resulted in the reduction of saturated fatty acids (P ≤ 0.05) by 14% in LS50 group and 17% in LS100 group and increase of unsaturated fatty acids (P ≤ 0.05) by 58% in LS50 group and 90% in LS100 group in muscle fat. The results clearly confirm that lupin-based diet increases the dietary value of chicken meat as one of the most important protein sources in human nutrition.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"312 22","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41279654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuchun Xie, Nai Rile, Xuewu Li, Haijun Li, Meng Zhao, Tianyu Che, Ting Cai, Zhi-hong Liu, Jinquan Li
The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed’s meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein–protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.
{"title":"Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness","authors":"Yuchun Xie, Nai Rile, Xuewu Li, Haijun Li, Meng Zhao, Tianyu Che, Ting Cai, Zhi-hong Liu, Jinquan Li","doi":"10.17221/61/2021-cjas","DOIUrl":"https://doi.org/10.17221/61/2021-cjas","url":null,"abstract":"The Inner Mongolia cashmere goat is famous for its bright white cashmere fibre. However, little attention is given to the excellent characteristics of this breed’s meat. We used label-free proteomics to analyse the total protein content in five different muscles, and 1 227 proteins were detected. Through sequential windowed acquisition of all theoretical fragment ions (SWATH), 16, 33, 49, 39, and 31 differentially expressed proteins were successfully detected in the five muscles. Protein–protein interaction network analysis of differentially expressed proteins revealed many strong interactions related to fatty acid beta oxidation and muscle development. Based on SWATH in five muscles, 25 differentially expressed proteins related to muscle development were detected, including seven muscle fibre structural proteins (ACTG2, ACTN4, TAGLN, MYL3, MYL1, MYL6B and MYH4). Finally, immunohistochemical analysis of MYL3 showed that the proportion of MYL3 may be a potential molecular marker for muscle toughness.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43017089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Sox transcription factor family plays an important role in various biological processes such as animal sex determination and multiple organ development. We used online databases to analyze the gene structure, chemical characteristics, and evolutionary relationship of Sox family genes through bioinformatics, and we studied the expression profiles and regulatory mechanisms of Sox family genes. A total of 29 rainbow trout Sox genes were identified. The phylogenetic analysis found that Sox genes of rainbow trout were clustered in seven subfamilies (B1, B2, C, D, E, F and H), and the gene structure of each subfamily was relatively conserved. Furthermore, Sox1, Sox4, Sox6, Sox8, Sox9, Sox11, Sox17, Sox18, and Sox19 developed into two copies, which might be the result of teleost fish-specific genome replication. Multiple HMG box domain alignments indicated that the motifs for all Sox sequences are conserved. Gene expression studies reveal that Sox expression is tissue-specific and that multiple Sox genes are involved in rainbow trout gonad and central nervous system development. Our study provides valuable information on the evolution of teleosts, and will also help to further research the functional characteristics of Sox genes.
{"title":"Evolution, characterization and expression analysis of Sox gene family in rainbow trout (Oncorhynchus mykiss)","authors":"Fang Ma, Yali Zou, Ruilin Ma, X. Chen, Lanfang Ma","doi":"10.17221/4/2022-cjas","DOIUrl":"https://doi.org/10.17221/4/2022-cjas","url":null,"abstract":"The Sox transcription factor family plays an important role in various biological processes such as animal sex determination and multiple organ development. We used online databases to analyze the gene structure, chemical characteristics, and evolutionary relationship of Sox family genes through bioinformatics, and we studied the expression profiles and regulatory mechanisms of Sox family genes. A total of 29 rainbow trout Sox genes were identified. The phylogenetic analysis found that Sox genes of rainbow trout were clustered in seven subfamilies (B1, B2, C, D, E, F and H), and the gene structure of each subfamily was relatively conserved. Furthermore, Sox1, Sox4, Sox6, Sox8, Sox9, Sox11, Sox17, Sox18, and Sox19 developed into two copies, which might be the result of teleost fish-specific genome replication. Multiple HMG box domain alignments indicated that the motifs for all Sox sequences are conserved. Gene expression studies reveal that Sox expression is tissue-specific and that multiple Sox genes are involved in rainbow trout gonad and central nervous system development. Our study provides valuable information on the evolution of teleosts, and will also help to further research the functional characteristics of Sox genes.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46921715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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