Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA最新文献

Objective: To study the localization of L-type calcium channel alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D in CA1 and CA3 regions of adult rat hippocampus.

Methods: Immunohistochemical staining was employed for specific labeling of alpha1 subunits CaV1.2alpha1C and CaV1.3alpha1D.

Results: CaV1.2alpha1C subunit was mainly located in the apical and basal dendrites in the CA1 area and the CA3 area, neuronal soma, basal dendrites and distal apical dendrites were all positively stained. In contrast to CaV1.2alpha1C, CaV1.3alpha1D displayed no obvious difference in immunostaining between CA1 and CA3, and was distributed mainly in the neuronal soma and proximal dendrites. At cellular level, CaV1.2alpha1C distribution showed a distinct clustered pattern while a uniform distribution was observed for CaV1.3alpha1D subunit.

Conclusion: Different alpha1 subunits of L-type calcium channel have differential expression in adult hippocampal neurons.

Objective: To study the effect of the serum of rabbits fed with Shengmai Chenggu capsule (SMCGC), a preparation of traditional Chinese medicine, on the proliferation and vascular endothelial growth factor (VEGF) secretion of cultured ECV304 cells.

Methods: The drug-containing serum from rabbits with intragastric administration of SMCGC at different doses was obtained for treatment of ECV304 cells cultured in vitro, with the cells treated with blank serum and ligustrazine serving as the control. The proliferation of ECV304 cells was detected by MTT assay and VEGF concentration in the culture medium of the cells measured by means of ELISA. The ultrastructure of ECV304 cells treated with the sera for 12 h was observed by transmission electron microscopy.

Results: After treatment with SMCGC-containing rabbit serum for 12 and 36 h, respectively, the proliferation of ECV304 cells in all dosage groups showed no significant increases (P>0.05), and the sera of rabbits fed with medium and high doses of SMCGC significantly inhibited the proliferation of ECV304 cells after a 36-hour treatment (P<0.01). The high-dose rabbit serum might even cause apoptosis of ECV304 cells, and low-dose serum significantly stimulated VEGF secretion of the ECV304 cells (P<0.01).

Conclusions: SMCGC does not promote the proliferation of ECV304 cells, and the serum of low-dose SMCGC-treated rabbits may stimulate VEGF secretion of the ECV304 cells, which might be the main mechanism of SMCGC in stimulating angiogenesis.

Objective: To investigate the clinical and imaging features of primary hepatic lymphoma (PHL).

Methods: Four PHL cases were analyzed retrospectively for clinical manifestations, transcatheter hepatic arterial angiography and CT features.

Results: All the four cases were B-cell non-Hodgkin lymphoma proven by histological and immunohistochemical examination. The main clinical manifestations included pain in the right upper quadrant and B symptoms of the lymphoma (fever, night sweating, and weight loss) in 3 patients. Three cases were complicated by chronic hepatitis or cirrhosis, and the other had renal transplantation two years ago. All these cases exhibited elevated serum lactate dehydrogenase (LDH) level, which was reduced after surgery or chemotherapy. Plain CT scan all identified hypodense lesions which did not display marked enhancement on the arterial phase and portal venous phase scans. On delayed phase scan, the border of the lesions became clear, and slight enhancement was observed in the peripheral and some partitions of the lesions. Angiographic imaging displayed slight tumor staining and arterial displacement in the liver in all the cases with thin tumor-supplying vessels. Global staining or abnormally thickened vessels were not seen.

Conclusion: A comprehensive evaluation of the clinical manifestations and imaging features can be helpful in the diagnosis of PHL, and serum LDH level may help to assess the therapeutic effect.

Objective: To elaborate on the diagnosis and treatment of blast injury of the lungs in extensively burned patients.

Methods: The clinical data of 16 patients with blast injury of the lungs and extensive burns were analyzed.

Results: The clinical manifestations of blast injury of the lungs were observed 7-10 days after the injury, with variable manifestations in chest X-ray. Five patients developed acute respiratory distress syndrome, and all the patients experienced pulmonary infection. Fourteen cases were finally cured, and 2 died of severe blast injury of the lungs and severe systemic infection. The burns healed well in all the surviving patients.

Conclusions: These patients often have delayed onset of clinical manifestation, severe symptoms of long duration, with high rate of pulmonary infection and pleural effusions, and variable manifestations in chest X-ray easily confused with pulmonary infections. Attention should be given to management of multiorgan dysfunction syndrome and adequate transfusion. Low tide volume lung-protective ventilation strategy and lung recruitment measures may prove beneficial. Prevention and management of ventilator-associated pneumonia, pulmonary infection and wound infection, along with improvement of microcirculation, organ function protection and intensive nutrition support, are effective treatments for patients with blast injury of the lungs and extensive burns.

Objective: To investigate the migration and differentiation of ependymal/subventricular zone cells after focal cerebral ischemia in rats, and reveal the origin of the newly generated neural cells in the peri-infarct region.

Methods: Normal adult male Sprague Dawley rats weighing 250-350 g were used in this study. Before middle cerebral artery occlusion (MCAO), 10 microl of 0.2% DiI was injected into the lateral ventricle for prelabeling the ependymal/subventricular zone cells. After ischemia, cumulative BrdU labeling was employed to detect the newly generated cells and double immunofluorescent staining to identify cell differentiation. The labeled cells were observed with laser confocal microscopy.

Results: In the non-ischemic control rats, DiI-labeled cells resided in the ependyma/subventricular zone. After focal cerebral ischemia, DiI-labeled cells were found in the corpus callosum, adjacent striatum and cortex, and some DiI/BrdU/glial fibrillary acidic protein (GFAP)-positive cells or DiI/BrdU/ neuronal nuclear antigen (NeuN)-positive cells were observed in the peri-infarct region in the striatum or cortex since day 14 after MCAO.

Conclusion: After focal cerebral ischemia, ependymal/subventricular zone cells migrate into the peri-infarct region where they differentiate into neurons and astrocytes. This finding may be important for understanding the source of adult neural stem cells and for developing new therapeutic intervention strategy through enhancing endogenous neurogenesis after brain injury.

Objective: To investigate the cytotoxicity of cytotoxic T lymphcytes (CTL) induced by keratin 19 (K19)-sensitized dendritic cells (DC) against MCF-7 cells in vitro.

Methods: DC isolated from peripheral blood mononuclear cells were cultured in vitro and sensitized by K19 peptide to generate specific CTL. The phenotypes and intracytoplasm cytokine of DC were analyzed by flow cytometry. Mixed leukocyte responses were evaluated by (3)H-TdR incorporation assay. The T cells were generated by DC pulsed with K19 antigen and T cell cytotoxicity was measured by (51)Cr release assay.

Results: The DCs derived from peripheral blood mononuclear cells expressed high levels of CD40, CD86, CD80 and CD83. Mixed leukocyte responses induced by the DCs pulsed with K19 was stronger than that induced by naive DCs (P<0.01). The cytotoxicity rate of CTL induced by the sensitized DCs was higher than that of CTL induced by naive DCs (P<0.01). The cytotoxity activity was enhanced by increasing the effector/target ratio (P<0.01).

Conclusion: After sensitization with K19 antigen, the DCs can stimulate T cell proliferation and induce cytotoxicity activity against MCF-7 cells. Increased effector/target ratio may enhance the cytotoxity activity. Sensitized DCs possess the potential for amplification in immunotherapy of carcinoma.

Objective: To investigate the correlation between spleen deficiency syndrome in colorectal carcinoma and bcl-2 gene expression, and observe the regulatory effect of Jianpikangfu decoction.

Methods: Forty-five advanced colorectal carcinoma patients with spleen deficiency were randomized into Jianpikangfu decoction treatment group with also symptomatic treatment with western medicine and control group in which the patients were given expectant treatment with western medicine. The activity of salivary amylase and bcl-2 expression in the tumor tissues were detected before and after the treatment.

Results: Jianpikangfu decoction in combination with western medicine treatment produced more obvious inhibition of reduction in salivary amylase activity than exclusive western medicine treatment (t=7.822, P<0.01), and significantly lowered the positivity rate of bcl-2 expression (chi2=4.286, P<0.05) in the tumor tissues, which, however, displayed no obvious changes in response to exclusive western medicine treatment.

Conclusion: Jianpikangfu decoction can inhibit the decrease in salivary amylase activity and regulate bcl-2 gene expression in colorectal carcinoma patients with spleen deficiency syndrome.

Objective: To investigate the protective effects of polarizing cardioplegia with Na(+) channel inhibitor tetrodotoxin (TTX) in ex vivo rat heart preservation.

Methods: Using a Langendorff preparation, Wistar rat hearts were arrested and preserved with St. Thomas (STH) solution (n=10) or with polarizing cardioplegia (TTX, n=10) for 7 h in hypothermic storage (10 degrees Celsius). All the hearts then underwent 30 min of reperfusion. Pre-ischemia and post-ischemia indexes of the rat hearts were determined, including the hemodynamic parameters, myocardial enzymology, ATP content and ultrastructural changes.

Results: The recovery of hemodynamic parameters of the hearts in TTX group were better than those in STH group (P<0.01). In comparison with STH group, the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) in TTX group was significantly lower (P<0.05) and ATP level maintained a relative high level with better protected myocardial ultrastructure.

Conclusion: TTX polarizing cardioplegia provides more effective long-term hypothermic preservation of isolated rat hearts than STH.

Objective: To assess the significance of human papillomavirus (HPV) DNA detection in the pelvic lymph nodes in cervical cancer.

Methods: HPV L1 gene fragment was amplified by HPV-specific PCR with general consensus primers from cervical cancer tissues. The types of HPV were identified by sequencing of the PCR product. The relationship between HPV positivity and pathological findings in metastatic pelvic lymph nodes of cervical cancer was analyzed.

Results: The positivity rate of HPV DNA was 40% in the pelvic lymph nodes in 40 cases of cervical cancer. HPV DNA was detected in the pelvic lymphatic nodes of all the 10 cases with pathologically confirmed lymph node metastasis of cervical cancer. HPV18 was detected in both of cervical and pelvic lymph nodes in some cases.

Conclusions: HPV DNA can be detected in the pelvic lymph nodes before pathological identification of lymph node metastasis and indicates early pelvic lymph node metastasis of cervical cancer. The presence of HPV18 often indicates high likeliness of lymph node metastasis and suggests poor prognosis of the patients.

Objective: To investigate the expression of survivin and its association with high-risk human papillomavirus (HPV) infection in cervical cancer and precancerous tissues.

Methods: In 76 patients with cervical intraepithelial neoplasia (CIN) or cervical carcinoma, the expression of survivin was detected by immunohistochemistry and the infection rates of high-risk HPV including HPV16, 18, 31, 33, 52, and 58 were assayed by touch-down PCR, with 10 cases of normal cervical specimens serving as the control.

Results: Survivin expression tended to increase along with the tumor progression (P<0.05), and was associated with the histological grade of invasive carcinoma (P<0.05) but not with the patients' age, clinical stage or histological classification (P>0.05). The positivity rate of high-risk HPV increased only in association with tumor progression (P<0.05) but not with the patients' age, clinical stage or histological classification (P>0.05). Positive correlation between the expression of survivin and high-risk HPV infection was observed in cervical carcinoma.

Conclusion: Survivin might play an important role in the occurrence and development of cervical carcinoma in coordination with high-risk HPV.