Domestic animal endocrinology最新文献

Elevated anti-müllerian hormone concentrations in blood serum of dogs with oligozoospermia or azoospermia 少精症或无精症犬血清抗<s:1>勒氏杆菌激素浓度升高

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2026-01-09 DOI: 10.1016/j.domaniend.2026.106994

Beate Walter , Jessica Cremer , Laura Meder , Eva-Maria Bauer , Sven Reese , Christiane Otzdorff

Reduced semen quality such as oligozoospermia or azoospermia is a frequent reason for subfertility in dogs. Anti-Müllerian hormone (AMH), produced by Sertoli cells, is known to play a role in spermatogenesis. Elevated serum AMH concentrations have been reported in dogs with Deslorelin-induced testicular atrophy resulting in azoospermia. This led us to hypothesize that congenital or acquired oligozoospermia or azoospermia often due to testicular degeneration or atrophy may also result in increased AMH serum levels, and that AMH could potentially serve as a diagnostic marker. The study included 14 dogs diagnosed with oligo- or azoospermia and twenty-nine proven sires served as controls. All dogs underwent a physical and andrological examination, semen evaluation, and blood collection for serum AMH measurement. In the dogs with oligo- or azoospermia, a testicular ultrasound was undertaken, and in twelve serum testosterone was measured. Additionally, pathohistological examination following castration was carried out in four dogs, and in two of these, immunohistochemical analysis using anti-AMH antibodies was performed. Serum AMH concentrations in dogs with oligo- or azoospermia were significantly higher than in the control group (p < 0.001, power: 99.98 %), with no overlap in values. These results suggest that oligo- or azoospermia in dogs goes along with an increase in serum AMH concentration, comparable to other testicular disorders such as Sertoli cell tumors or cryptorchidism from which it has to be distinguished.

精子质量下降，如少精子症或无精子症是狗生育能力低下的常见原因。已知由支持细胞产生的抗勒氏激素（AMH）在精子发生中起作用。血清AMH浓度升高已报道在狗与地洛林诱导睾丸萎缩导致无精子症。这使我们假设先天性或获得性少精子症或无精子症通常是由于睾丸变性或萎缩，也可能导致AMH血清水平升高，AMH可能作为一个潜在的诊断标志物。该研究包括14只诊断为少精症或无精症的狗和29只被证实的狗作为对照。所有的狗都接受了生理和男科检查，精液评估和血清AMH测定的血液采集。对少精或无精犬进行睾丸超声检查，并对12只犬进行血清睾酮测定。此外，对4只狗进行了阉割后的病理组织学检查，并对其中两只狗进行了抗amh抗体的免疫组织化学分析。少精或无精犬血清AMH浓度显著高于对照组（p < 0.001，功率：99.98%），值无重叠。这些结果表明，狗的少精症或无精症伴随着血清AMH浓度的增加，这与其他睾丸疾病（如支持细胞瘤或隐睾症）相似，必须将其与之区分开来。

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引用次数: 0

Dehydroepiandrosterone sulfate as a potential biomarker for assessing the etiology of canine naturally occurring Cushing’s syndrome 脱氢表雄酮硫酸盐作为评估犬自然发生库欣综合征病因的潜在生物标志物。

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-12-20 DOI: 10.1016/j.domaniend.2025.106993

Inês Oliveira , Patrícia Marques , Francesca Del Baldo , Mariachiara Re , Luísa Mateus , Rodolfo Oliveira Leal

Canine naturally occurring Cushing’s syndrome (CS) is usually caused by a pituitary tumor (pituitary-dependent hypercortisolism; PDH) or a functional adrenocortical tumor (adrenal-dependent hypercortisolism; ADH). Although endogenous adrenocorticotropic hormone (eACTH) measurement combined with imaging tests is one of the most reliable methods for differentiating PDH from ADH, it has some limitations. In humans, dehydroepiandrosterone sulfate (DHEAS) levels may help determine CS etiology. This study aimed to assess the role of DHEAS concentrations in differentiating PDH from ADH in dogs and correlate DHEAS and eACTH concentrations. A multicenter, retrospective, cross-sectional study was conducted, including dogs diagnosed with CS. They were divided into groups according to suspected CS etiology, sex and neutered status. A total of 38 dogs were included; 33/38 (87 %) were classified as having PDH and 5/38 (13 %) ADH. There were 19 females and 19 males, of which 16 and 9 were neutered, respectively. Median DHEAS concentrations were significantly higher in intact males (2.45 ng/mL; interquartile range 1.02-7.80 ng/mL) than in neutered males (0.68 ng/mL; interquartile range 0.42-2.03 ng/mL; p = 0.017). Females showed a positive correlation between DHEAS and eACTH levels (r = 0.588; p = 0.008). There were no significant differences in DHEAS concentrations between PDH and ADH (p = 0.645). Moreover, ROC curve analysis demonstrated poor ability of DHEAS to differentiate PDH from ADH (AUC 0.430). In conclusion, DHEAS does not appear to be a relevant biomarker for determining CS etiology in dogs and ACTH may not be a major driver for its synthesis.

犬自然发生的库欣综合征（CS）通常由垂体瘤（垂体依赖性高皮质醇症，PDH）或功能性肾上腺皮质瘤（肾上腺依赖性高皮质醇症，ADH）引起。虽然内源性促肾上腺皮质激素（acth）测量结合影像学检查是鉴别PDH和ADH最可靠的方法之一，但它也有一定的局限性。在人类中，硫酸脱氢表雄酮（DHEAS）水平可能有助于确定CS的病因。本研究旨在评估DHEAS浓度在狗体内区分PDH和ADH中的作用，并将DHEAS和acth浓度联系起来。进行了一项多中心，回顾性，横断面研究，包括诊断为CS的狗。根据疑似CS病因、性别和绝育情况分组。总共包括38只狗；其中33/38（87%）为PDH， 5/38（13%）为ADH。雌性19只，雄性19只，其中绝育16只，绝育9只。完整雄鼠DHEAS浓度中位数（2.45 ng/mL，四分位数范围1.02 ~ 7.80 ng/mL）显著高于绝育雄鼠（0.68 ng/mL，四分位数范围0.42 ~ 2.03 ng/mL, p = 0.017）。女性DHEAS与acth水平呈正相关（r = 0.588; p = 0.008）。PDH与ADH的DHEAS浓度差异无统计学意义（p = 0.645）。ROC曲线分析显示，DHEAS区分PDH和ADH的能力较差（AUC为0.430）。总之，DHEAS似乎不是确定犬CS病因的相关生物标志物，ACTH可能不是其合成的主要驱动因素。

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引用次数: 0

HDAC5 mediates the regulation of muscle-derived interleukin 6 on the differentiation of porcine intramuscular pre-adipocytes HDAC5介导肌源性白细胞介素6对猪肌内前脂肪细胞分化的调节。

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-12-05 DOI: 10.1016/j.domaniend.2025.106992

Weimin Lin, Hongbing Song, Chongfan Du, Jing Wang, Xiaohan Zhang, Chu Yang, Tianfang Xiao, Ruiyi Lin

Intramuscular fat (IMF) deposition is a critical determinant of pork quality, with pre-adipocyte differentiation serving as a pivotal regulatory process. Although muscle-derived interleukin-6 (IL-6) is recognized for its role in promoting lipolysis and fatty acid oxidation, the epigenetic mechanisms governing its regulation remain poorly characterized. This study elucidates the epigenetic regulation of histone deacetylase 5 (HDAC5) on muscle-derived IL-6 transcription and secretion, and its consequent effects on porcine IMF deposition. Western blot (WB) and quantitative PCR (qPCR) analyses demonstrated that elevated IL-6 expression in the porcine longissimus dorsi muscle was negatively correlated with IMF content. Chromatin immunoprecipitation (ChIP)-PCR and adipogenic induction assays revealed that HDAC5 suppresses IL-6 transcription and secretion, thereby enhancing intramuscular pre-adipocyte differentiation. Through co-immunoprecipitation (Co-IP), dual-luciferase reporter assays, and differentiation induction experiments, we identified a functional interaction between HDAC5 and myocyte enhancer factor 2A (MEF2A). Mechanistically, MEF2A binds to the IL-6 promoter to activate its transcription and secretion, which subsequently inhibits pre-adipocyte differentiation. Dual-interference assays further confirmed that HDAC5 regulates IL-6 expression by directly binding to and repressing MEF2A activity, ultimately modulating IMF deposition. These findings uncover a novel epigenetic pathway through which HDAC5 governs muscle-derived IL-6 to regulate pre-adipocyte differentiation and IMF accumulation in pigs, offering critical molecular insights for strategies aimed at optimizing pork quality.

肌内脂肪（IMF）沉积是猪肉品质的关键决定因素，脂肪细胞前分化是关键的调节过程。尽管肌肉来源的白介素-6 （IL-6）被认为在促进脂肪分解和脂肪酸氧化中起作用，但其调控的表观遗传机制仍不清楚。本研究阐明了组蛋白去乙酰化酶5 （HDAC5）对肌源性IL-6转录和分泌的表观遗传调控及其对猪IMF沉积的影响。Western blot （WB）和定量PCR （qPCR）分析表明，猪背最长肌中IL-6的表达升高与IMF含量呈负相关。染色质免疫沉淀(ChIP)-PCR和脂肪诱导实验显示，HDAC5抑制IL-6的转录和分泌，从而增强肌内前脂肪细胞分化。通过共免疫沉淀（Co-IP）、双荧光素酶报告基因检测和分化诱导实验，我们发现了HDAC5和肌细胞增强因子2A （MEF2A）之间的功能相互作用。在机制上，MEF2A结合IL-6启动子激活其转录和分泌，随后抑制前脂肪细胞分化。双干扰实验进一步证实HDAC5通过直接结合并抑制MEF2A活性调控IL-6表达，最终调控IMF沉积。这些发现揭示了一种新的表观遗传途径，通过HDAC5控制肌肉来源的IL-6来调节猪的前脂肪细胞分化和IMF积累，为优化猪肉质量的策略提供了关键的分子见解。

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引用次数: 0

Functional luteal tissue formation and progesterone production following preovulatory follicle aspiration and follicular cell restoration in ewes 母羊排卵前卵泡抽吸和卵泡细胞恢复后黄体组织的形成和黄体酮的产生

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-12-04 DOI: 10.1016/j.domaniend.2025.106991

Renan Denadai , Fernanda Fagali Franchi , Rodrigo Garcia , Leticia Cristina Salgado , Nuno Emanuel Oliveira Figueiredo Silva , Yeda Fumie Watanabe , Paula Barreto da Rocha , Eunice Oba , John Patrick Kastelic , João Carlos Pinheiro Ferreira , Sony Dimas Bicudo

The objective was to monitor corpus luteum (CL) development after preovulatory follicle aspiration, with or without intrafollicular autologous follicular cell restoration. Eighteen ewes were synchronized by inserting an intravaginal pessary with 60 mg medroxyprogesterone acetate on day -16, inducing luteolysis with cloprostenol (140 µg, im) on day -12, followed by pessary withdrawal and equine chorionic gonadotropin (400 IU, im) on day -2. Forty-eight hours later, ewes were assigned to Control (CO; n = 5), Laparoscopic aspiration (LA; n = 6), or Laparoscopic aspiration + follicular cell restoration (FCR; n = 7) treatments. Daily ultrasonography assessed the largest individual CL cross-sectional area (CLA) and total luteal sectional area (TLA), and blood samples were collected every 48 h to assess plasma progesterone concentrations. Luteal formation was observed in all ewes. However, the LA group exhibited a higher number of CLs compared to the CO group (1.7 ± 0.5 vs. 1.0 ± 0.0; P < 0.05). Nevertheless, neither CO nor LA differed from the FCR group (1.4 ± 0.2; P = 0.10 and P = 0.60, respectively). In addition, TLA values were similar among the CO, LA, and FCR groups (0.8 ± 0.04, 0.9 ± 0.05, and 1.0 ± 0.05 cm², respectively; P = 0.50) and plasma progesterone concentrations did not differ significantly (4.0 ± 0.31, 3.7 ± 0.30, and 2.9 ± 0.30 ng/mL, respectively; P = 0.30). In conclusion, preovulatory follicle aspiration induces luteal tissue formation, which, with or without intrafollicular autologous follicular cell restoration, results in functional CL capable of producing progesterone levels consistent with those normally observed during the estrous cycle.

目的是监测排卵前卵泡穿刺后黄体（CL）的发展，有或没有卵泡内自体卵泡细胞修复。18只母羊在第-16天插入含有60 mg醋酸甲孕酮的阴道内子宫托，在第-12天用氯前列醇（140 μ g, im）诱导黄体溶解，然后在第2天停用子宫托并使用马绒毛膜促性腺激素（400 IU, im）。48小时后，将母羊分为对照组（CO, n = 5）、腹腔镜抽吸组（LA, n = 6）和腹腔镜抽吸+滤泡细胞修复组（FCR, n = 7）。每日超声检查评估最大个体CL截面积（CLA）和总黄体截面积（TLA），每48 h采血评估血浆黄体酮浓度。所有母羊均观察到黄体形成。然而，与CO组相比，LA组显示出更高的CLs数量（1.7±0.5 vs 1.0±0.0;P < 0.05）。然而，CO和LA与FCR组均无差异（1.4±0.2;P = 0.10和P = 0.60）。此外，CO、LA和FCR组的TLA值相似（分别为0.8±0.04、0.9±0.05和1.0±0.05 cm²，P = 0.50），血浆孕酮浓度差异无统计学意义（分别为4.0±0.31、3.7±0.30和2.9±0.30 ng/mL, P = 0.30）。综上所述，排卵前卵泡抽吸诱导黄体组织形成，无论是否进行卵泡内自体卵泡细胞修复，都能产生与发情周期中正常观察到的黄体酮水平一致的功能性CL。

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引用次数: 0

Pilot study of β-endorphin concentrations in horses with pituitary pars intermedia dysfunction using a newly validated enzyme-linked immunosorbent assay 使用新验证的酶联免疫吸附试验对垂体部中间功能障碍马体内β-内啡肽浓度的初步研究

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-11-26 DOI: 10.1016/j.domaniend.2025.106982

N. Fouché , J. Howard , V. Gerber , P. Billmann , M. Farinha do Sul , G. Christen , R. Bruckmaier , C. Philipona , N. Besuchet Schmutz , J. Gross

β-endorphin, a proopiomelanocortin (POMC)-derived peptide secreted by pars intermedia melanotropes, may play a significant but underexplored role in pituitary pars intermedia dysfunction (PPID) pathophysiology and diagnosis. This study aimed to (1) validate a commercially available human β-endorphin enzyme-linked immunosorbent assay (ELISA) kit for equine samples, and (2) compare β-endorphin concentrations between horses with PPID and healthy controls. Assay validation included the generation of standard curves using purified synthetic equine β-endorphin and human β-endorphin standards, with both curves showing full parallelism. Intra- and inter-assay coefficients of variation (CV) were determined by measuring 37 equine serum samples in duplicate on a single plate and five serum samples across seven different plates. The intra-assay CV was 11.3 % for standards and 5.3 % for equine samples, whereas the inter-assay CV was 6.9 % for standards and 15.6 % for equine samples. Plasma β-endorphin concentrations remained stable over 24 hours regardless of centrifugation timing, storage temperature, or duration. β-endorphin concentrations were determined in five horses with PPID and 20 healthy aged controls. Horses in the PPID group had significantly higher β-endorphin concentrations (median, 506 pg/mL; IQR, 213–762) compared to horses in the control group (median, 35 pg/mL; IQR, 16–55) (P < 0.001). This study may serve as a basis for further research on the role of β-endorphin in horses, particularly in horses with PPID.

β-内啡肽（β-endorphin）是一种由垂体中间部黑色素异色体分泌的促黑素原（POMC）衍生肽，可能在垂体中间部功能障碍（PPID）的病理生理和诊断中发挥重要作用，但尚未得到充分的研究。本研究旨在(1)验证市售的用于马样品的人β-内啡肽酶联免疫吸附测定（ELISA）试剂盒，以及(2)比较患有PPID的马和健康对照的β-内啡肽浓度。实验验证包括用纯化的合成马β-内啡肽和人β-内啡肽标准品生成标准曲线，两种曲线完全平行。测定内变异系数和测定间变异系数（CV）是通过在单个板上重复测量37份马血清样本和在7个不同板上测量5份血清样本来确定的。标准品的测定内CV为11.3%，马样品的测定内CV为5.3%，而标准品的测定间CV为6.9%，马样品的测定间CV为15.6%。血浆β-内啡肽浓度在24小时内保持稳定，无论离心时间、储存温度或持续时间如何。测定了5匹PPID马和20匹健康老年对照马的β-内啡肽浓度。PPID组马的β-内啡肽浓度（中位数，506 pg/mL； IQR, 213-762）显著高于对照组马（中位数，35 pg/mL； IQR, 16-55）（P < 0.001）。本研究可为进一步研究β-内啡肽在马，特别是PPID马中的作用奠定基础。

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引用次数: 0

The effects of different trace mineral supplementation strategies on performance, feeding behavior, health, carcass quality, and profitability of mineral deficient receiving steers 不同微量矿物质补充策略对缺矿接收阉牛生产性能、摄食行为、健康、胴体品质和盈利能力的影响

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-11-20 DOI: 10.1016/j.domaniend.2025.106981

Tevan J. Brady , Ryan Feuz , Caleb C. Reichhardt , Laura A. Motsinger , Nikole E. Ineck , Reganne K. Briggs , Lillian L. Okamoto , Ian Sroufe , John Ferry , Marcus McGee , Brett R. Bowman , Matthew D. Garcia , Ryan Larsen , E. Bart Tarbet , Kerry A. Rood , Kara J. Thornton

This research aimed to determine best practices when receiving mineral deficient cattle. To do this, forty steers were stratified by initial weight and liver mineral status into one of four treatments: no mineral supplementation (CON; n=10), oral supplementation of mineral at concentrations similar to NASEM requirements (AM; n=10), oral supplementation of minerals above NASEM requirements (HM; n=10), or a MultiMin® injection at labeled dose (MM; n=10) for the first 40 d of the receiving period. Treatments were only given for the first 40 d to determine best practices for beef producers to follow to regain sufficient mineral concentration when receiving mineral deficient calves. All steers received the same ration in covered pens with Vytelle® bunks. Standing liver biopsies were collected on d 0, 5, 10, 20, 30 and 40 and analyzed for mineral content. Feedlot performance (average daily gain, dry matter intake, and gain:feed) was recorded. After the trial, steers were harvested commercially and carcass data was obtained. There were no treatment differences (P>0.10) in feedlot performance, carcass traits or cost of gain. When analyzed over time, HM increased (P<0.001) liver copper and selenium compared to all other treatments, but did not alter (P>0.10) manganese or zinc. Steers in the HM treatment had an increased (P<0.05) number and time duration of bunk visits/feed bouts, and had decreased (P<0.05) consumption per bunk visit/feed bout and time with their head down per bunk visit/feed bout compared to other treatments. No effects (P>0.10) of treatment were seen on bovine herpes virus or bovine parainfluenza virus 3 antibody titers. These data indicate that different mineral supplementation strategies don’t affect performance or health, but may impact feeding behavior and liver mineral concentrations.

这项研究旨在确定接收缺乏矿物质的牛的最佳做法。为此，根据初始体重和肝脏矿物质状况，将40只阉牛分为四组：不补充矿物质（CON, n=10），口服矿物质（AM, n=10），口服矿物质（HM, n=10），高于NASEM要求的矿物质（HM, n=10），或在接受期的前40天按标记剂量注射MultiMin®（MM, n=10）。仅在头40天进行处理，以确定牛肉生产者在接收缺乏矿物质的小牛时应遵循的最佳做法，以恢复足够的矿物质浓度。所有阉牛在带Vytelle®工位的有盖围栏中接受相同的口粮。分别于第0、5、10、20、30和40天进行肝脏活检，分析矿物质含量。记录饲养场生产性能（平均日增重、干物质采食量和增重比）。试验结束后，对肉牛进行商业化捕捞并获得胴体数据。在饲养场生产性能、胴体性状和增重成本方面，各处理无显著差异（P>0.10）。随着时间的推移进行分析，与所有其他治疗相比，HM增加了肝铜和硒（P> 0.001），但没有改变锰或锌（P>0.10）。与其他治疗组相比，HM治疗组的牛每次上铺/喂食次数和持续时间增加（P<0.05），每次上铺/喂食次数消耗和每次下铺/喂食时间减少（P<0.05）。治疗对牛疱疹病毒或牛副流感病毒3抗体滴度没有影响（P>0.10）。这些数据表明，不同的矿物质补充策略不会影响生产性能或健康，但可能会影响摄食行为和肝脏矿物质浓度。

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引用次数: 0

Effects of castration on growth performance and pineal gland function of Hu sheep 去势对湖羊生长性能和松果体功能的影响

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-11-05 DOI: 10.1016/j.domaniend.2025.106980

Wanhong Li, Haiyu Ma, Peidi Zhao, Xiuxiu Weng

Castration, as a veterinary procedure, can enhance carcass quality, significantly influencing the development of male animals. This study aimed to assess the effect of castration on growth performance and pineal gland function in Hu sheep lambs. Six healthy male Hu sheep were selected and randomly assigned to two groups. Three lambs underwent castration at 3 days of age (CAS), whereas the control group (CK) remained uncastrated. All lambs were housed indoors. Results indicated that the CAS group's body weight was significantly lower than that of the CK group (p < 0.05). However, the feed conversion ratio was significantly higher in the CAS group than in the CK group (p < 0.05). Serum testosterone, growth hormone and melatonin levels in the CK group were significantly elevated compared with those in the CAS group (p < 0.05). Pineal glands were harvested for RNA sequencing, which revealed 537 differentially expressed genes in the CAS group compared with CK, comprising 174 upregulated and 363 downregulated genes. The Kyoto Encyclopedia of Genes and Genomes pathway analysis highlighted significant enrichment in the neural ligand–receptor interactions, and autoimmune thyroid disease. The results demonstrated that castration could significantly influence the growth and development of lambs and affect the function of the pineal gland.

阉割作为一种兽医手术，可以提高胴体质量，对雄性动物的发育有显著影响。本试验旨在探讨去势对湖羊羔羊生长性能和松果体功能的影响。选取健康雄性湖羊6只，随机分为2组。3日龄（CAS）阉割3只羔羊，对照组（CK）不阉割。所有的羔羊都被圈养在室内。结果表明，CAS组体重显著低于CK组（p < 0.05）。但CAS组的饲料系数显著高于CK组（p < 0.05）。与CAS组相比，CK组血清睾酮、生长激素和褪黑素水平显著升高（p < 0.05）。采集松果体进行RNA测序，与CK相比，CAS组差异表达基因537个，其中上调基因174个，下调基因363个。京都基因和基因组百科全书通路分析强调了神经配体-受体相互作用和自身免疫性甲状腺疾病的显著富集。结果表明，去势对羔羊的生长发育和松果体功能有显著影响。

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引用次数: 0

Immunogenicity of recombinant gonadotropin-releasing hormone contraceptive vaccines in male pigs 重组促性腺激素释放激素避孕疫苗在猪体内的免疫原性

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-11-01 DOI: 10.1016/j.domaniend.2025.106979

Jean Vitor Bondavalli , Monike Willemin Quirino , Neida Conrad , Vanessa Peripolli , Elizabeth Schwegler , Matheus Folgearini Silveira , Yuso Tutida , Bernardo Garziera Gasperin , Fábio Pereira Leivas Leite , Ivan Bianchi , Fabiana Moreira

This study assessed the contraceptive effects of two vaccines formulated with recombinant gonadotropin-releasing hormone antigen (rGnRH antigen) conjugated with the heat-labile enterotoxin B subunit (LTB) protein from Escherichia coli in male pigs. Thirty-two piglets were assigned to four groups: Surg, surgically castrated at 3 days old; Vvx, non-castrated and vaccinated with a commercial vaccine (Vivax®) at 101 and 135 days old; rGnRH-400 and rGnRH-300, non-castrated and vaccinated at 101 and 135 days old with recombinant vaccines containing 400 µg or 300 µg rGnRH/LTB per dose, respectively. Growth performance was not affected by treatment (p ≥ 0.25). At 155 and 165 days old, piglets in the rGnRH-400 and rGnRH-300 groups exhibited anti-GnRH/LTB antibody levels were similar to those in the Vvx group (p > 0.05) or higher (p < 0.05) than those in the Vvx group. However, rGnRH-400 and rGnRH-300 piglets showed higher testosterone concentrations at 155 and 165 days old and greater testicular volumes at 165 days old than Vvx piglets (p < 0.05). Severe testicular lesions (scores 3 and 4) were more frequent in the Vvx group, while those in the rGnRH-400 group were mainly scored as 3, and only mild lesions (scores 1 and 2) were observed in the rGnRH-300 group. Overall, both recombinant vaccines elicited strong anti-GnRH/LTB antibody levels; however, they did not produce the same degree of testicular alterations or testosterone reduction as the commercial vaccine. Further studies are warranted to evaluate the contraceptive potential of these recombinant formulations, particularly the 400 µg rGnRH/LTB vaccine.

本研究评估了两种由重组促性腺激素释放激素抗原（rGnRH抗原）结合大肠杆菌热不稳定肠毒素B亚基（LTB）蛋白配制的疫苗对雄性猪的避孕效果。将32头仔猪分为4组：手术组，即3日龄手术阉割组；Vvx，未阉割，在101和135天大时接种了商业疫苗（Vivax®）；rGnRH-400和rGnRH-300，未阉割，分别在101和135天龄时接种每剂量含有400µg或300µg rGnRH/LTB的重组疫苗。生长性能不受处理影响（p≥0.25）。155和165日龄时，rGnRH-400和rGnRH-300组仔猪抗gnrh /LTB抗体水平与Vvx组相近（p > 0.05）或高于Vvx组（p < 0.05）。而rGnRH-400和rGnRH-300仔猪在155和165日龄时睾酮浓度和睾丸体积均高于Vvx仔猪（p < 0.05）。重度睾丸病变（3分和4分）在Vvx组多见，rGnRH-400组以3分为主，rGnRH-300组仅出现轻度病变（1分和2分）。总的来说，这两种重组疫苗都激发了很强的抗gnrh /LTB抗体水平；然而，它们并没有产生与商业疫苗相同程度的睾丸改变或睾丸激素减少。需要进一步的研究来评估这些重组制剂的避孕潜力，特别是400µg的rGnRH/LTB疫苗。

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引用次数: 0

Effects of protein-bound iron supplementation on reproductive performance of sows and growth performance of piglets 添加蛋白结合铁对母猪繁殖性能和仔猪生长性能的影响

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-10-31 DOI: 10.1016/j.domaniend.2025.106978

Hongxuan Ye , Junyi Wu , Xuexun Feng , Qiling Chen , Zihao Huang , Bin Su , Songfeng Yang , Lili Wang , Hefeng Luo , Chengquan Tan

Iron is an essential nutrient for maternal health and fetal development. 65 multiparous sows were used to determine the effects of maternal protein-bound iron (P-Iron) supplementation on reproductive performance of sows and growth of their offspring. Sows received either the farm’s control diet (total iron 180 mg/kg, including 80 mg/kg as ferrous sulfate; n = 32) or the same diet plus 120 mg Fe/kg as protein-bound iron (P-Iron; n = 33) from gestation day 85 to lactation day 21. The results showed that, relative to the control (CON), sows in the P-Iron group had higher hemoglobin at gestation day 110 and at farrowing (both P < 0.05), and the P-Iron group exhibited improved antioxidant status, with lower serum malondialdehyde at farrowing (P < 0.01), higher total antioxidant capacity (P < 0.05), and a trend toward higher glutathione (P = 0.08). Newborn piglets in the P-Iron group had higher hemoglobin concentration (P < 0.01). Reproductive performance in the P-Iron group showed a downward trend in mummified fetuses (P = 0.07). During lactation, colostrum and ordinary milk iron concentrations were higher in the P-Iron group than in CON (P < 0.05). At weaning, piglets from the P-Iron group had higher serum iron and iron saturation (both P < 0.05) and higher IgA levels (P < 0.05). Litters in the P-Iron group also had greater weaning litter weights (P < 0.05), with a trend toward improved survival (P = 0.09).Overall, dietary supplementation with 120 mg/kg P-Iron enhanced iron transfer from sows to offspring, resulting in increased hemoglobin levels in sows and newborn piglets, improved oxidative stress status, elevated iron and IgA concentration in serum, and ultimately higher litter weaning and pre-weaning survival rate.

铁是母亲健康和胎儿发育的必需营养素。以65头产母母猪为试验对象，研究了添加蛋白结合铁（P-Iron）对母猪繁殖性能和子代生长的影响。母猪从妊娠第85天至泌乳第21天分别饲喂猪场对照饲粮（总铁180 mg/kg，其中80 mg/kg为硫酸亚铁，n = 32）或相同饲粮中添加120 mg/kg的蛋白结合铁（P-Iron, n = 33）。结果表明，与对照组相比，P-铁组母猪妊娠第110天和分娩时血红蛋白较高（P < 0.05）， P-铁组母猪抗氧化能力较好，分娩时血清丙二醛较低（P < 0.01），总抗氧化能力较高（P < 0.05），且有提高谷胱甘肽水平的趋势（P = 0.08）。P-铁组新生仔猪血红蛋白浓度较高（P < 0.01）。P-铁组胎儿的生殖性能呈下降趋势（P = 0.07）。泌乳期间，P铁组初乳铁和乳铁浓度均高于CON组（P < 0.05）。断奶时，磷铁组仔猪血清铁和铁饱和度较高（P < 0.05）， IgA水平较高（P < 0.05）。P-铁组仔猪断奶窝重显著增加（P < 0.05），存活率有提高的趋势（P = 0.09）。综上所述，饲粮中添加120 mg/kg磷铁可以促进铁从母猪到后代的传递，从而提高母猪和新生仔猪的血红蛋白水平，改善氧化应激状态，提高血清中铁和IgA浓度，最终提高仔猪断奶和断奶前存活率。

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引用次数: 0

Treatment with chorionic gonadotropins during lactation inhibits post-weaning estrus expression in sows 哺乳期间使用绒毛膜促性腺激素抑制母猪断奶后发情表达。

IF 2.1 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology

Pub Date : 2025-10-17 DOI: 10.1016/j.domaniend.2025.106977

Arthur Martelli , Monike Willemin Quirino , Michele Dezordi Franz , Vanessa Peripolli , Fabiana Moreira , Bernardo Garziera Gasperin , Rafael da Rosa Ulguim , Vilceu Bordignon , Thomaz Lucia Jr , Ivan Bianchi

This study evaluated the efficiency of protocols using two different dosages of eCG and hCG administered during lactation to delay post-weaning estrus expression in sows. Sixty-two sows were selected on D-14 (D0 = weaning) and allocated to one of three treatments: Control (n = 20; saline administration on D-7 and D-4); 500 IU (n = 21; 500 IU eCG on D-7 and 500 IU hCG on D-4); and 1000 IU (n = 21; 1000 IU eCG on D-7 and 1000 IU hCG on D-4). Estrus detection was performed twice daily after weaning, and blood samples were collected on D-7, D-1, D+6, and D+13. On D+15, the sows were slaughtered for ovarian evaluation. The percentage of sows detected in estrus post-weaning was greater in the Control group (90.0%) compared to the 500 IU (23.8%) and 1000 IU (9.5%) groups (P < 0.01). The proportion of sows with corpus hemorrhagicum and/or corpus luteum and the total number of corpora lutea at slaughter were similar among treatments (P ≥ 0.41). On D+6, serum progesterone (P4) concentration was lower in the Control group than those in the 500 IU and 1000 IU groups (P < 0.01). Administration of 500 or 1000 IU of eCG and hCG during lactation effectively induced the formation of corpora lutea and sustained high serum P4 levels for at least 13 d post-weaning, thereby inhibiting estrus expression in 76 to 90 % of treated sows.

本研究评估了在哺乳期间使用两种不同剂量的eCG和hCG延迟母猪断奶后发情表达的方案的效率。在D-14日（D0 =断奶）选取62头母猪，分为3组：对照组（n = 20， D-7和D-4给予生理盐水）；500iu (n = 21， D-7心电图500iu， D-4 hCG 500iu)；1000 IU （n = 21； D-7心电图1000 IU， D-4 hCG 1000 IU）。断奶后每日2次进行发情检测，于D-7、D-1、D+6、D+13采集血样。D+15屠宰母猪进行卵巢评价。断奶后母猪发情检出率，对照组（90.0%）高于500 IU组（23.8%）和1000 IU组（9.5%）（P < 0.01）。屠宰时出现出血体和（或）黄体的母猪比例和黄体总数在各处理间无显著差异（P≥0.41）。D+6时，对照组血清孕酮（P4）浓度低于500、1000 IU组（P < 0.01）。哺乳期间给予500或1000 IU eCG和hCG可有效诱导黄体的形成，并在断奶后至少13 d内维持高血清P4水平，从而抑制76 - 90%的处理母猪的发情表达。

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引用次数: 0