Current Biology最新文献

Matheson et al. introduce the Iberian ribbed newt (Pleurodeles waltl), a species of salamander that lives some of its adult life on land and some in water, requiring remarkable physiological and behavioral plasticity to adapt to these very different environments.

Recognizing conspecifics-others of the same species-in order to determine how to interact with them appropriately is a fundamental goal of animal sensory systems. It has undergone selective pressure in nearly all species. Mice have a large repertoire of social behaviors that are the subject of a rapidly growing field of study in neuroscience. Mouse social interactions likely incorporate all available sensory modalities, and the vast majority of studies have not attempted to isolate them. Our understanding of the role of vision in mouse social interactions remains overlooked, given the prominence of olfactory research in this area. To address this, we developed a behavioral platform that allowed us to present a subject mouse with the visual information of stimulus mice in isolation from olfactory, acoustic, and tactile cues. Our results indicate that the visual identification of the sex or individual identity of other mice influences behavior. These findings highlight the underappreciated role of vision in mouse social interactions and open new avenues to study the visual circuits underlying social behavior.

The regulation of mitotic spindle positioning and orientation is central to the morphogenesis of developing embryos and tissues.^1,2,3,4,5 In many multicellular contexts, cell geometry has been shown to have a major influence on spindle positioning, with spindles that commonly align along the longest cell shape axis.^{6,7,8,9,10,11,12,13,14} To date, however, we still lack an understanding of how the nature and amplitude of intracellular forces that position, orient, or hold mitotic spindles depend on cell geometry. Here, we used in vivo magnetic tweezers to directly measure the forces that maintain the mitotic spindle in the center of sea urchin cells that adopt different shapes during early embryo development. We found that spindles are held by viscoelastic forces that progressively increase in amplitude as cells become more elongated during early development. By coupling direct cell shape manipulations in microfabricated chambers with in vivo force measurements, we establish how spindle-associated forces increase in dose dependence with cell shape anisotropy. Cytoplasm flow analysis and hydrodynamic simulations suggest that this geometry-dependent mechanical enhancement results from a stronger hydrodynamic coupling between the spindle and cell boundaries, which dampens cytoplasm flows and spindle mobility as cells become more elongated. These findings establish how cell shape affects spindle-associated forces and suggest a novel mechanism for shape sensing and division positioning mediated by intracellular hydrodynamics with functional implications for early embryo morphogenesis.

Conservation initiatives strive for reliable and cost-effective species monitoring.^1,2,3 However, resource constraints mean management decisions are overly reliant on data derived from single methodologies, resulting in taxonomic or geographic biases.⁴ We introduce a data integration framework to optimize species monitoring in terms of spatial representation, the reliability of biodiversity metrics, and the cost of implementation, focusing on tigers and their principal prey (sambar deer and wild pigs). We combined information from unstructured ranger patrols, systematic sign transects, and camera traps in Sumatra's largest remaining tropical forest and used integrated community occupancy models to analyze this multifaceted dataset in a unified way. Data integration improved the precision of species occupancy estimates by 14%-42%, enhanced the accuracy of species inferences, expanded the spatial scope of inference to the landscape level, and cut operational costs up to 51-fold. Our framework demonstrates the underappreciated value of integrating unstructured observations with monitoring data derived from traditional wildlife surveys.

Diverse eukaryotic cells assemble microtubule networks that vary in structure and composition. While we understand how cells build microtubule networks with specialized functions, we do not know how microtubule networks diversify across deep evolutionary timescales. This problem has remained unresolved because most organisms use shared pools of tubulins for multiple networks, making it difficult to trace the evolution of any single network. In contrast, the amoeboflagellate Naegleria expresses distinct tubulin genes to build distinct microtubule networks: while Naegleria builds flagella from conserved tubulins during differentiation, it uses divergent tubulins to build its mitotic spindle. This genetic separation makes for an internally controlled system to study independent microtubule networks in a single organismal and genomic context. To explore the evolution of these microtubule networks, we identified conserved microtubule-binding proteins and used transcriptional profiling of mitosis and differentiation to determine which are upregulated during the assembly of each network. Surprisingly, most microtubule-binding proteins are upregulated during only one process, suggesting that Naegleria uses distinct component pools to specialize its microtubule networks. Furthermore, the divergent residues of mitotic tubulins tend to fall within the binding sites of differentiation-specific microtubule regulators, suggesting that interactions between microtubules and their binding proteins constrain tubulin sequence diversification. We therefore propose a model for cytoskeletal evolution in which pools of microtubule network components constrain and guide the diversification of the entire network, so that the evolution of tubulin is inextricably linked to that of its binding partners.

During tissue morphogenesis, an interplay of biochemical pathways and mechanical cues regulates polarized cell behaviors, the balance of which leads to tissues reaching their correct shape and size.^1,2,3,4 A well-studied example of a biochemical regulator is the highly conserved Fat-Dachsous (Ft-Ds) pathway that coordinates planar polarized cell behaviors and growth in epithelial tissues.^5,6 For instance, in the Drosophila larval wing disc, the Ft-Ds pathway acts via the atypical myosin Dachs to control tissue shape by promoting the orientation of cell divisions primarily in a proximodistal (PD) direction.^7,8 Here, we investigate interactions between Ft-Ds planar polarity and mechanical forces in the developing Drosophila pupal wing. We show that in the early stages of pupal wing development (16-18 h after puparium formation), anteroposterior (AP)-oriented cell divisions and T1 transitions are controlled by the Ft-Ds pathway acting via Dachs. Shortly thereafter, PD-oriented tissue tension is induced across the wing blade by the process of hinge contraction. This opposes the control of Dachs over polarized cell behaviors in a tug-of-war fashion, resulting in more PD-oriented cell divisions and T1s. Furthermore, increased PD tissue tension stabilizes Ft along PD-oriented junctions, suggesting that biomechanical feedback on the Ft-Ds pathway resists the effects of hinge contraction on cell shape. We also show that loss of Dachs results in increased myosin-II stability at cell junctions, revealing compensatory interactions between these two myosins. Overall, we propose that Ft-Ds pathway function constitutes a mechanism whereby tissues are buffered against mechanical perturbations.

A new global analysis shows that wildfires turn temperate and boreal forests into major emitters of greenhouse gases - instead of storing carbon. Without sustainable forest fire management, forest fires may amplify climate change, leading to irreversible ecological changes.

The endoplasmic reticulum (ER) is a continuous cellular endomembrane network that displays focal specializations. Most notable examples of such specializations include the spine apparatus of neuronal dendrites and the cisternal organelle of axonal initial segments. Both organelles exhibit stacks of smooth ER sheets with a narrow lumen, interconnected by a dense protein matrix. The actin-binding protein synaptopodin is required for their formation, but the underlying mechanisms remain unknown. Here, we report that the spine apparatus and synaptopodin are conserved from flies to mammals and that a highly conserved region of this protein is necessary, but not sufficient, for its association with ER. We reveal a dual role of synaptopodin in generating actin bundles and in linking them to the ER. Expression of a synaptopodin construct constitutively anchored to the ER in non-neuronal cells is sufficient to generate stacked ER cisterns resembling the spine apparatus. Cisterns within these stacks are molecularly distinct from the surrounding ER and are connected to each other by an actin-based matrix that contains proteins also found at the spine apparatus of neuronal spines. Our findings shed light on mechanisms governing the biogenesis of this peculiar structure and represent a step toward understanding the elusive properties of this organelle.

Skin-penetrating nematodes infect nearly one billion people worldwide. The developmentally arrested infective larvae (iL3s) seek out hosts, invade hosts via skin penetration, and resume development inside the host in a process called activation. Activated infective larvae (iL3as) traverse the host body, ending up as parasitic adults in the small intestine. Skin-penetrating nematodes respond to many chemosensory cues, but how chemosensation contributes to host seeking and intra-host navigation-two crucial steps of the parasite-host interaction-remains poorly understood. Here, we investigate the role of carbon dioxide (CO₂) in promoting host seeking and intra-host navigation in the human-infective threadworm Strongyloides stercoralis. We show that S. stercoralis exhibits life-stage-specific behavioral preferences for CO₂: iL3s are repelled, non-infective larvae and adults are neutral, and iL3as are attracted. CO₂ repulsion in iL3s may prime them for host seeking by stimulating dispersal from host feces, while CO₂ attraction in iL3as may direct worms toward high-CO₂ areas of the body, such as the lungs and intestine. We also identify sensory neurons that detect CO₂; these neurons display CO₂-evoked calcium activity, promote behavioral responses to CO₂, and express the receptor guanylate cyclase Ss-GCY-9. Finally, we develop an approach for generating stable knockout lines in S. stercoralis and use this approach to show that Ss-gcy-9 is required for CO₂-evoked behavioral responses in both iL3s and iL3as. Our results highlight chemosensory mechanisms that shape the interaction between parasitic nematodes and their human hosts and may aid in the design of novel anthelmintics that target the CO₂-sensing pathway.

Worldwide declines in the abundance of non-pest insects threaten ecosystems, food production, and human wellbeing. A large-scale study has systematically examined field and environmental levels of 1,024 agrochemicals and the effect of small temperature increases. The results are disturbing.