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Lipids peroxidation of wood plants seedlings under the volatile organic compounds action 木本植物幼苗脂质过氧化作用下挥发性有机物的作用
Pub Date : 2019-09-20 DOI: 10.15421/031920
E. G. Tyulkova, L. P. Avdashkova
One of the criteria for plant adaptation to the volatile organic compounds action can be the lipid peroxidation intensity processes as a result of destructive oxidative processes ratio and the plants activity antioxidant protection. In this regard, the aim of the study was a comparative study of volatile various doses organic compounds effect (o-xylol, benz(a)pyrene, butyl acetate) on the content of malondialdehyde (MDA) in woody plants seedlings under specified experimental conditions. The results of an experiment on processing leaf blades of urban environments woody plants seedlings with volatile hydrocarbons and their mixture indicate that the effect of o-xylol, butyl acetate and a mixture of butyl acetate and o-xylol on the malonic dialdehyde content in drooping birch Betula pendula Roth. during the first days after exposure, it was manifested in the accumulation of lipid peroxidation products, whereas after three days of the experiment, the plant response in this case was expressed as a decrease in the content of MDA and the intensity of the lipid peroxidation process. Benz(a)pyrene in drooping birch caused a higher content of MDA three days after treatment. In the pyramidal poplar, the character of o-xylol influence and a butyl acetate and o-xylol mixture over the experiment course was similar to the drooping birch and was expressed in the lipid peroxidation products accumulation one day after treatment. Benz(a)pyrene was the cause of the increased malondialdehyde content one day after treatment, butyl acetate – after three days of the experiment. The combined processing of birch seedlings leaf blades with a butyl acetate and o-xylol mixture made it possible for these compounds to enhance each other's toxic effect compared to their action alone, with the exception of o-xylol three days after treatment with the mixture in the maximum dose. In pyramidal poplar, unlike birch, suspended butyl acetate in the mixture weakened the o-xylol toxic effect in comparison with its effect in the form of a single solution, and o-xylol weakened the effect of butyl acetate. In general, in the studied wood seedlings, the most potent compounds one cluster in the processes of membrane lipid peroxidation compared with the control can include benz(a)pyrene (in birch), butyl acetate and a mixture of butyl acetate and o-xylol (at the poplar pyramidal).
植物适应挥发性有机化合物作用的标准之一可能是由破坏性氧化过程比率和植物活性抗氧化保护引起的脂质过氧化强度过程。为此,本研究的目的是在规定的实验条件下,比较研究挥发性不同剂量的有机化合物(邻二甲苯、苯并(a)芘、乙酸丁酯)对木本植物幼苗丙二醛(MDA)含量的影响。用挥发性烃及其混合物处理城市环境木本植物叶片的实验结果表明,邻二甲苯、乙酸丁酯和乙酸丁酯与邻二甲苯的混合物对垂桦叶片丙二醛含量的影响。在暴露后的第一天,表现为脂质过氧化产物的积累,而在实验的第三天,这种情况下的植物反应表现为MDA含量的降低和脂质过氧化过程的强度。在处理后3天,下垂桦木的苯并(a)芘含量升高。在锥体杨树中,邻二甲苯的影响和乙酸丁酯与邻二甲苯混合物在试验过程中的特征与下垂桦树相似,表现为处理后1天的脂质过氧化产物积累。苯并(a)芘是处理后第一天丙二醛含量增加的原因,醋酸丁酯是处理后第三天丙二醛含量增加的原因。桦树幼苗叶片与乙酸丁酯和邻二甲苯混合物的联合处理使得这些化合物与单独作用相比,可以增强彼此的毒性作用,但邻二甲苯在用最大剂量的混合物处理后三天除外。与桦树不同的是,在锥体杨树中,悬浮液中的乙酸丁酯与单一溶液相比,对邻二甲苯的毒性作用减弱,邻二甲苯对乙酸丁酯的毒性作用减弱。总的来说,在研究的木材幼苗中,与对照相比,膜脂过氧化过程中最有效的化合物簇包括苯并芘(桦树)、乙酸丁酯和乙酸丁酯与邻二甲苯的混合物(杨树金字塔)。
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引用次数: 1
Hydrological and hydrobotanic typology of the lake of North-Steppe Dnieper region 第聂伯河北草原地区湖泊的水文和水植物类型学
Pub Date : 2019-09-20 DOI: 10.15421/031921
N. Roshchyna, B. Baranovski
This article is devoted to the typology of lakes of the North-Steppe Dnieper. In developing the typology of lakes, the parameters were taken into account: landscape location, hydro-chemical and hydro-biological characteristics and the degree of their anthropogenic transformation. The data presented are based on the processing of stationary and route research materials from 1998 to 2018 on the lakes of river valleys: Dnieper, Samara, and Orel. Hydrological indicators are analyzed according to the literature, cartographic and archival data of the Dneprodiprovodkhoz Institute and the Biology Research Institute of Oles Honchar Dnipropetrovsk National University. Floristic studies were carried out using general botanical methods of collection and herbarization, and in the study of typical aquatic flora - special hydro-botanical methods. Geo-botanical studies were carried out according to geo-botanical and special hydro-botanical methods. The article presents the hydrological and hydro-botanical features of the lakes of the valley of a large river (Dnieper) and medium rivers (Samara, Orel). Lakes are located exclusively in valley-terrace landscapes in the northern part of the steppe zone of Ukraine. Despite this, based on cartographic materials, we proposed zoning of the territory of the lakes of the North-Steppe Dnieper according to the criteria: their location in lake regions, in various landscapes and the degree of anthropogenic transformation. The following districts and subareas were identified: Dnieper Lake District (Dnieper floodplain lake subarea with slight flooding of the floodplain, Dnieper Lake subarea of floodplain terraces, Dievsky floodplain lake subarea); Samara Lake District (Lake Subarea of Samara Coniferous forest, Lake Subarea of Estuary part of Samara); Orel Lake District. Lakes are located in various physical and geographical conditions of the floodplain, arena and third saline terrace. The typology of the lakes of the North-Steppe Dnieper basin was developed on the basis of regionalization of the location of the lakes, distribution according to the ecological and topographic profile, hydrological, hydro-chemical regimes, degree and nature of overgrowing. 11 types of lakes are identified based on the above criteria. 6 types were identified for the valley of a large river: floodplain lakes (3 types) with a long-flow regime, lakes of the second (sandy) terrace (2 types) and highly mineralized lakes of the third (saline) terrace. 5 types were identified for the valleys of middle rivers: floodplain lakes (3 types) with an episodic short-burial regime, lakes of the second (sandy) terrace (low-mineralized) and excessively mineralized lakes of the third (saline) terrace.
这篇文章致力于第聂伯河北部草原湖泊的类型学。在开发湖泊类型时,考虑了景观位置、水化学和水生物特征及其人为转化程度等参数。所提供的数据是基于1998年至2018年对第聂伯河、萨马拉和奥廖尔河谷湖泊的固定和路线研究材料的处理。水文指标是根据第聂伯罗彼得罗夫斯克国立大学第聂伯罗彼得罗夫斯克国立大学第聂伯罗彼得罗夫斯克国立大学第聂伯罗彼得罗夫斯克国立大学生物研究所的文献、地图和档案数据进行分析的。植物区系研究采用一般的植物采集和草药化方法,典型水生植物区系研究采用特殊的水植物学方法。地植物学研究是根据地植物学和特殊的水植物学方法进行的。本文介绍了大河(第聂伯河)和中等河流(萨马拉河、奥廖尔河)流域湖泊的水文和水文植物学特征。湖泊完全位于乌克兰草原区北部的山谷阶地景观中。尽管如此,基于地图资料,我们建议对第聂伯河北部草原湖泊的领土进行分区,根据这些标准:它们在湖泊地区的位置,在各种景观中的位置以及人为改变的程度。确定了第聂伯河湖区(第聂伯河漫滩轻度泛洪湖泊分区、第聂伯河漫滩阶地分区、迪耶夫斯基漫滩湖泊分区);萨马拉湖区(萨马拉针叶林湖泊分区、萨马拉河口部分湖泊分区);奥廖尔湖区。湖泊分布在各种自然地理条件下的洪泛平原、竞技场和第三盐碱地。第聂伯河北草原流域湖泊的类型学是根据湖泊的地理位置、生态和地形剖面、水文、水化学制度、过度生长的程度和性质划分的。根据上述标准确定了11种湖泊类型。在某大型河流流域,确定了6种类型的湖泊:具有长流状态的洪泛平原湖泊(3种)、第二阶地(砂质)湖泊(2种)和第三阶地(盐质)高度矿化的湖泊。在中游河谷中,发现了5种类型:具有幕式短埋藏制度的洪泛区湖泊(3种)、第二阶地(砂质)湖泊(低矿化)和第三阶地(盐碱地)过度矿化湖泊。
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引用次数: 1
Variability of morphometric characteristics of Betula pendula lamina under technogenic conditions of Dnipro city 第聂伯罗市技术条件下钟白桦叶片形态计量特征的变异
Pub Date : 2019-09-17 DOI: 10.15421/031912
T. Yusypiva, A. Zadesenets, A. V. Karas
The response of a plant organism to the effects of negative environmental factors, including the effects of multicomponent pollution, can be determined using a morphometric approach, setting the range of variability of biometric indicators of assimilation organs. The obtained data allow us to identify the directions of evolution of leaf shape and parameters under conditions of anthropogenic environment. The purpose of this work is to evaluate the variability of morphometric characteristics of lamina of Вetula pendula Roth. under technogenic conditions of the city of Dnipro. Leaf samples were collected in September – October 2018 at five test areas in the city of Dnipro (100 sheets each), which differed in the level of environmental pollution. For each leaf according to the method, five dimensions were measured on the left and right sides: width of the left and right halves of the leaf; the length of the vein of the second order, the second from the base of the leaf; the distance between the bases of the first and second veins of the second order; the distance between the ends of the first and second veins of the second order; the angle between the main vein and the second vein from the base of the leaf of the second order. It is shown that by the degree of increase of variation in the conditions of influence on the plants of B. pendula under conditions of multicomponent contamination of the environment the investigated signs of the lamina can be arranged as follows: 5 < 2 < 1 < 4 < 3. It is established that the most stable feature of the B. pendula lamina in the conditions of technogenesis is the angle between the main vein and the second vein from the base of the leaf of the second order, and the most variable one is the distance between the bases of the first and second veins of the second order. A tendency was found for the differences in the reactions of plants from the relatively clean area and contaminated territories: the control trees had a coefficient of variation higher for the right half of the leaf (right-sided asymmetry), whereas plants of the technogenic zones had left-sided asymmetry.
植物有机体对负面环境因素的反应,包括多组分污染的影响,可以使用形态计量学方法确定,设置同化器官生物计量指标的变异性范围。得到的数据使我们能够确定在人为环境条件下叶片形状和参数的演化方向。本研究的目的是评估Вetula罗斯钟摆(pendula Roth)椎板形态计量学特征的可变性。在第聂伯罗市的技术条件下。2018年9月至10月,在第聂伯罗市的五个试验区(每个试验区100张)收集了叶子样本,这些试验区的环境污染程度不同。根据该方法,在每片叶子的左右两侧测量了五个维度:叶子的左右半部分的宽度;第二级叶脉的长度,第二级从叶基部开始;第二脉第一脉和第二脉底之间的距离;第二级的第一脉和第二脉末端之间的距离;叶脉与叶脉之间的夹角,叶脉与叶脉之间的夹角。结果表明,在环境多组分污染条件下,根据影响条件的变化程度,所调查的叶片标志可排列为:5 < 2 < 1 < 4 < 3。结果表明,在技术发生条件下,垂叶叶片最稳定的特征是主脉与第二脉距叶片基部的夹角,变化最大的特征是第一脉与第二脉距叶片基部的距离。研究发现,相对清洁区和污染区植物的反应存在差异:对照树的右半叶变异系数较高(右侧不对称),而技术区植物的左侧不对称。
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引用次数: 2
Influence of low fire on condition of pine plants 低火对松树生长状况的影响
Pub Date : 2019-06-19 DOI: 10.15421/031922
V. Vdovychenko, D. O. Omelich
Fires have caused significant damage since ancient times, and over time the area and damage has increased. Pine plantations in the southern and eastern regions of Ukraine are particularly affected by this natural phenomenon. The damage caused by the fire interrupts the course of natural successions and prolongs the period of climax. In order to assess the impact of persistent grassroots fire on the complex of forestry and taxation indices of plantations, the taxation structure of pine plantations and the impact on phytocenoses, a methodical analysis of plantations in the Skripayivsky forestry of the state enterprise «Skripayevsky Educational Research Forestry» was made. The data of forest fires over the last 30 years have been analyzed and the reconnaissance works have been carried out, the characteristic test areas have been selected and monitored over the three years. As a result of the work, the relationship between the height of the slope and the intensity of drying was observed. It has been observed that the mass drying of trees does not occur in the first year, but within three years. Also noted was a certain category of trees that appeared to be more resistant to the negative effects of fire, characterized by the following characteristics: thick, dense, high raised bark, well cleared of branches of tree trunks, diameter above average, high raised crown. This factor should be taken into account when creating forest crops (use quality planting material and the introduction of deciduous trees), carrying out further planting care (timely care of forest crops and the required intensity) and carrying out fire prevention measures (raising crowns, mineralization and mineralization breaks). Based on these observations, it is possible to predict the state of planting after a fire, depending on the intensity of the fire (altitude and the degree of burning of the litter) and take into account when conducting forestry activities.
自古以来,火灾就造成了巨大的破坏,随着时间的推移,火灾的面积和损害都在增加。乌克兰南部和东部地区的松树种植园尤其受到这种自然现象的影响。火灾造成的破坏中断了自然演替过程,延长了高潮期。为了评估持续的基层火灾对林业和人工林税收指数的影响,松树人工林的税收结构和对植物的影响,对国有企业“斯克里帕耶夫斯基教育研究林业”的斯克里帕耶夫斯基林业的人工林进行了系统分析。对近30年来的森林火灾数据进行了分析,开展了勘察工作,选取了特色试验区,进行了3年的监测。作为工作的结果,观察到坡度的高度和干燥强度之间的关系。据观察,树木的大量干燥不是在第一年发生,而是在三年内发生。还注意到某一类树木似乎对火的负面影响更有抵抗力,其特点是:厚、密、高凸起的树皮、树干上的分枝清除得很好、直径高于平均水平、高凸起的树冠。在创造森林作物(使用优质种植材料和引进落叶乔木)、进行进一步的种植护理(及时护理森林作物和所需的强度)和实施防火措施(提高树冠、矿化和矿化断裂)时应考虑到这一因素。根据这些观察结果,可以根据火灾的强度(海拔高度和凋落物的燃烧程度)预测火灾后的种植状态,并在开展林业活动时予以考虑。
{"title":"Influence of low fire on condition of pine plants","authors":"V. Vdovychenko, D. O. Omelich","doi":"10.15421/031922","DOIUrl":"https://doi.org/10.15421/031922","url":null,"abstract":"Fires have caused significant damage since ancient times, and over time the area and damage has increased. Pine plantations in the southern and eastern regions of Ukraine are particularly affected by this natural phenomenon. The damage caused by the fire interrupts the course of natural successions and prolongs the period of climax. In order to assess the impact of persistent grassroots fire on the complex of forestry and taxation indices of plantations, the taxation structure of pine plantations and the impact on phytocenoses, a methodical analysis of plantations in the Skripayivsky forestry of the state enterprise «Skripayevsky Educational Research Forestry» was made. The data of forest fires over the last 30 years have been analyzed and the reconnaissance works have been carried out, the characteristic test areas have been selected and monitored over the three years. As a result of the work, the relationship between the height of the slope and the intensity of drying was observed. It has been observed that the mass drying of trees does not occur in the first year, but within three years. Also noted was a certain category of trees that appeared to be more resistant to the negative effects of fire, characterized by the following characteristics: thick, dense, high raised bark, well cleared of branches of tree trunks, diameter above average, high raised crown. This factor should be taken into account when creating forest crops (use quality planting material and the introduction of deciduous trees), carrying out further planting care (timely care of forest crops and the required intensity) and carrying out fire prevention measures (raising crowns, mineralization and mineralization breaks). Based on these observations, it is possible to predict the state of planting after a fire, depending on the intensity of the fire (altitude and the degree of burning of the litter) and take into account when conducting forestry activities.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84897689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wastewater bioremediation with using of phototrophic non-sulfur bacteria Rhodopseudomonas yavorovii IMV B-7620 雅氏红假单胞菌IMV B-7620的光养非硫细菌生物修复废水
Pub Date : 2019-04-24 DOI: 10.15421/031911
O. Tarabas, S. Hnatush, O. Moroz, M. Kovalchuk
Phototrophic purple non-sulfur bacteria (PNSB) mineralize organic waste. In media with different organic substances they are metabolized from simple aliphatic organic acids to complex polysaccharides. These bacteria can produce H2, indole-3-acetic acid and 5-aminolevulinic acid. PNSB purify waters from hydrogen sulfide and, as components of ecosystem trophic chains, participate in process of sulfur cycle. The ability of these bacteria to denitrification is of particular interest to specialists because nitrates concentrations in industrial effluents are often much higher than the maximum permissible concentrations. The use of industrial, agricultural and household wastes to produce PNSB biomass is economically profitable. Therefore, the aim of our work was to investigate the influence of wastewater of the yeast plant and the water of the settler № 3 filtrate of the Lviv Solid Household Waste Landfill (LSHWL) on the biomass accumulation by PNSB Rhodopseudomonas yavorovii IMV B-7620 and the changes in the composition of these waters in the process of cultivation of the investigated bacteria. Bacteria were grown for 21 days anaerobically at an illumination intensity of 200 lux in 500 ml flasks at +29 ... +30 °C. The wastewater of the yeast plant and the water of the settler № 3 filtrate of the LSHWL were used as the cultivation media. Water was diluted at 10; 30; 50; 100 times. In water, diluted at 10 times, the pH, total ferrum content, Ca2+, Mg2+, Cl-, NO3-, NO2-, SO42-, HS- and chemical oxygen demand (COD) were determined. The biomass of bacteria was measured turbidimetrically at λ = 660 nm. The concentration of HS- was determined spectrophotometrically (λ = 665 nm) by the formation of methylene blue. The content of sulfate ions was determined turbidimetrically (λ = 520 nm) after their precipitation with barium chloride. The concentrations of Ca2+ and Mg2+ were determined titrometrically by reaction with trilon B; Na+ and K+ were analyzed by flame emission spectrometry method (lK = 768 nm, lNa = 589 nm). The pH of the water was measured potentiometrically. The concentration of Cl- was determined titrometrically by reaction with AgNO3. The concentration of nitrite ions was determined spectrophotometrically by the method of sulfanilic acid diazotization by nitrite ions and the interaction of the formed salt with n-(naphthyl)ethylenediamine dihydrochloride (λ = 540 nm, l = 10 mm). The concentration of nitrate ions was determined spectrophotometrically by the diazotization method. Zinc powder was used as the reducing agent. COD was measured with using of KMnO4. It is characterised the patterns of biomass accumulation by R. yavorovii IMV B-7620 bacteria in the wastewater of the yeast plant and the water of the settler № 3 filtrate of the LSHWL, diluted at 10–100 times. The optimal dilution (at 10 times) of water for bacteria cultivation was selected. Bacteria R. yavorovii IMV B-7620 accumulate biomass of 2.2 g/l during growth in a yeast plant wastewate
光养紫色无硫细菌(PNSB)矿化有机废物。在含有不同有机物质的培养基中,它们从简单的脂肪族有机酸代谢为复杂的多糖。这些细菌可以产生H2、吲哚-3-乙酸和5-氨基乙酰丙酸。PNSB净化水体中的硫化氢,作为生态系统营养链的组成部分,参与硫循环过程。专家对这些细菌的反硝化能力特别感兴趣,因为工业废水中的硝酸盐浓度往往远远高于最大允许浓度。利用工业、农业和家庭废物生产PNSB生物质具有经济效益。因此,我们的工作目的是研究酵母厂废水和利沃夫生活垃圾填埋场(LSHWL) 3号沉淀池滤液水对PNSB雅沃罗红假单胞菌IMV B-7620生物量积累的影响,以及这些水在培养过程中组成的变化。在光照强度为200勒克斯的500 ml烧瓶中,在+29…+ 30°C。以酵母菌厂废水和LSHWL 3号沉淀池滤液为培养基。水被稀释到10;30;50;100次。在10倍稀释的水中,测定pH、总铁含量、Ca2+、Mg2+、Cl-、NO3-、NO2-、SO42-、HS-和化学需氧量(COD)。在λ = 660 nm处用浊度法测定细菌生物量。通过亚甲基蓝的形成,分光光度法(λ = 665 nm)测定了HS-的浓度。用氯化钡沉淀硫酸根离子,用浊度法(λ = 520 nm)测定硫酸根离子的含量。与万亿B反应,用滴定法测定Ca2+和Mg2+的浓度;采用火焰发射光谱法(lK = 768 nm, lNa = 589 nm)分析Na+和K+。用电位法测定了水的pH值。通过与AgNO3反应,用滴定法测定了Cl-的浓度。采用亚硝酸盐离子磺胺重氮法和与n-萘乙二胺二盐酸盐(λ = 540 nm, l = 10 mm)相互作用法测定亚硝酸盐离子的浓度。用重氮化法分光光度法测定硝酸盐离子浓度。采用锌粉作为还原剂。用KMnO4测定COD。研究了酵母厂废水和LSHWL沉降器3号滤液(稀释10-100倍)中yavorovii IMV B-7620细菌生物量积累的模式。选择细菌培养用水的最佳稀释倍数(10倍)。细菌R. yavorovii IMV B-7620在酵母厂废水中生长期间积累的生物量为2.2 g/l,该废水是生物量的两倍,由细菌在沉淀剂3号滤液的稀释水中积累。在培养第21天,酵母厂废水和LSHWL沉淀池3号滤液的有机物氧化效率(化学需氧量测定)分别为42.3%和65.6%。R. yavorovii IMV B-7620影响废水中总Fe、Ca2+、Mg2+、NO3-、NO2-、SO42-、Cl-、HS-含量的可能性的确立,补充和拓宽了对紫色非硫菌在生态系统中作用的认识,紫色非硫菌可以显著影响自然界中这些化合物的生物地球化学循环。所得结果可为开发利用这些微生物处理硫化氢、氯、铁、氮、硫化合物废水的有效生物技术奠定基础。
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引用次数: 1
Ecological features of urease activity distribution in technogenically altered soils of the Nikopol manganese ore basin 尼科波尔锰矿盆地技术蚀变土壤脲酶活性分布的生态特征
Pub Date : 2019-04-05 DOI: 10.15421/031902
V. Chorna, I. Wagner
The challenge of degradation of natural ecosystems because of human activity is considered by the world community to the most serious problems facing mankind. As a result of mineral extraction, man-made landscapes and environmentally ruined areas replace natural habitats and agroecosystems; a whole spectrum of man-made processes are typical for such landscapes, which leads to a decrease in species richness and biological diversity within such areas. Degraded territories formed in the process of coal mining are often partially restored through remediation measures. During the implementation of the technical stage of remediation, substrates with different potential fertility having different environmental properties and quality are used. Specific features of distribution total, available nitrogen concentrations and levels of urease enzymatic activity at the layers of artificial soil, sod-lithogenic soils onto gray-green and red-brown clays and on loess-like loams in the Nikopol manganese ore basin are established. It is presented general assessment of technosol status by concentration of general easily hydrolysed nitrogen enzyme activity of urease and this enzyme enriches the soil with mineral nitrogen in the process of mineralization of organic substances.  The level of activity of urease,  investigated soils, determines the intensity of the direction of biochemical processes that affect soil fertility.High correlation between concentrations of soluble nitrogen and urease activity values by layers of artificial soil (r = 0.81), sod-lithogenic soils onto gray-green (r = 0.98), red-brown clays (r = 0.72) and onto loess-like loam (r = 0.85) were found. Tendency of decreasing hydrolytic enzyme activity, urease, with depth in all types of artificial soil studied was established. It has been established that biochemical diagnostics of technozem processes beyond the level of activity of the hydrolytic enzyme urease makes it possible to estimate the direction of changes at the layers of artificial soil, sod-lithogenic soils onto gray-green and red-brown clays and on loess-like loams. The recultivated soil has some what lower fertility and greater salimity at the lower horizons, but is capable of performing ecological functions and can not only be used for economic purposes, but also perform ecological functions.
由于人类活动造成的自然生态系统退化的挑战被国际社会认为是人类面临的最严重的问题。由于矿物开采,人造景观和环境破坏地区取代了自然栖息地和农业生态系统;这类景观的典型特征是一系列人为过程,导致这些地区物种丰富度和生物多样性的减少。在煤炭开采过程中形成的退化土地往往通过修复措施得到部分恢复。在修复技术阶段的实施过程中,使用具有不同环境特性和质量的不同潜在肥力的基质。建立了Nikopol锰矿盆地人工土壤、灰绿色和红棕色粘土和类黄土壤土上的草质岩质土壤的分布总量、有效氮浓度和脲酶酶活性水平的具体特征。通过尿素酶易水解酶活性的浓度对技术土壤状况进行一般性评价,该酶在有机质矿化过程中为土壤提供了丰富的矿质氮。土壤中脲酶的活性水平决定了影响土壤肥力的生化过程方向的强度。人工土壤(r = 0.81)、灰绿色土壤(r = 0.98)、红棕色土壤(r = 0.72)和类黄土壤土(r = 0.85)上不同层间的可溶性氮浓度与脲酶活性值呈高度相关。在所有类型的人工土壤中,水解酶活性随深度的增加而降低。已经确定的是,超越水解酶脲酶活性水平的技术过程的生化诊断,可以估计人工土壤层的变化方向,在灰绿色和红棕色粘土和黄土样壤土上的草质岩质土壤。复耕土壤在下层土壤肥力较低,土壤含盐量较高,但具有一定的生态功能,既可用于经济用途,又可用于生态功能。
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引用次数: 0
Transformation of the community of nesting birds in the process of reorganization of the forest ecosystem into a park 在改造筑巢鸟类群落的过程中,将森林生态系统改造成公园
Pub Date : 2019-03-20 DOI: 10.15421/031901
V. Gaychenko, T. Shupova
Changes in nesting conditions cause a change in the number of individual species, ecological groups, and, consequently, the structure of the bird community. The purpose of the study is to analyze the possibilities and directions – the transformations of the communitys of nesting birds in the process of reorganization of the forest ecosystem into a park. The material was collected in the territory of one of the forest parks in the Kiev city. Forest is an mixed based on Quercus robur L., Carpinus betulus L., Acer platanoides L., Tilia cordata L., Fraxinus excelsior L. There are individual trees Pinus Syvestris L. The territory of the forest park have different degrees transformed, and includes anthropogenic landscapes and forests plots that have been preserved almost in their natural state. Studies were conducted in the nesting period (April-June) 2012–2017. For analysis average data were used. The number and distribution of the birds were determined by the method of counting on the transects, in the 3 model plots. There are 71 species of birds of 11 orders. Of these, 63 species are nesting. The number of nested species of communities in model plots does not depend on the gradient of anthropogenic transformation, but a average density of nesting birds decrease: 2.91±0.66→2.54±0.67→2.10±0.48 pairs / km of the transect. Nesting birds are distributed between 9 faunogenetic complexes. Of these, in each model plot is represented by 8. Dominated by birds of the European nemoral complex. On the gradient of habitat transformation, a change in the fauna-genetic structure of bird communities in the direction of increasing the proportion of birds of the European nemoral and European forest-steppe complexes, birds of desert-mountain and tropical groups. Boreal and ancient species are superseded. Strengthening the transformation in forest plots, the distribution of birds in community on the ecological groups does change not much. But when the forest ecosystem is replaced by a park ecosystem, the proportion of sclerophiles increases 3–4 times. In all communities is dominated by woody nesters bird. On the gradient of transformation, the number of species of the tree canopies nesters birds (from 21 to 18), ground nesters birds (from 6 to 0), species that use many types of nesting stations (from 4 to 1) are decreases; the number of bird species that nesting in the buildings increases from 0 to 6, and the number of alien species from 0 to 4 (Streptopelia decaocto, Dendrocopos syriacus, Phoenicurus ochruros, Serinus serinus). In the park ecosystems, a decrease in the species composition of woodpeckers, does not entail a significant decrease in the species composition and abundance of secondary hollows nesters birds. Birds of the synanthropic subpopulations are nesting in cavities in park buildings. Most of the indices show an equivalent ά-diversity of all bird communities. The β-diversity of breeding birds during the transformation of the forest ecosystem into a park
筑巢条件的变化会导致单个物种、生态类群数量的变化,从而导致鸟类群落结构的变化。研究的目的是分析在森林生态系统重组为公园的过程中,筑巢鸟类群落转变的可能性和方向。这些材料是在基辅市的一个森林公园内收集的。森林以栎、桦木、枫槭、天麻、黄曲柳为主要树种,有单株松树。森林公园的范围发生了不同程度的变化,包括人为景观和森林样地,基本保持了自然状态。研究时间为2012-2017年巢期(4 - 6月)。分析采用平均数据。在3个模型样地中,采用样地上计数法确定鸟类的数量和分布。鸟类有11目71种。其中63种正在筑巢。模式样地的筑巢鸟类数量不受人为转换梯度的影响,但平均密度呈下降趋势:2.91±0.66→2.54±0.67→2.10±0.48对/ km。筑巢鸟分布在9个动物遗传复合体之间。其中,在每个模型图中用8表示。以欧洲神经复合体鸟类为主。在生境转变的梯度上,鸟类群落的区系遗传结构的变化方向为欧洲草原和欧洲森林草原复合体、沙漠-山地和热带类群鸟类的比例增加。北方和古代种被取代。加强林样改造,群落内鸟类在生态类群上的分布变化不大。但当森林生态系统被公园生态系统取代时,嗜硬菌的比例增加了3-4倍。在所有群落中以木巢鸟为主。在转换的梯度上,树冠筑巢鸟的种数从21种减少到18种,地面筑巢鸟的种数从6种减少到0种,使用多种筑巢站的种数从4种减少到1种;在建筑物内筑巢的鸟类由0种增加到6种,外来鸟类由0种增加到4种(十尾链蝇、叙利亚石斛、桔黄腓、丝蚕)。在公园生态系统中,啄木鸟物种组成的减少并不会导致次生空心巢鸟类的物种组成和丰度的显著减少。合栖亚群的鸟类在公园建筑物的洞穴中筑巢。大多数指数显示所有鸟类群落的多样性相当。在森林生态系统向公园的转变过程中,繁殖鸟类的β-多样性减少了一半。物种丰度的排序分布曲线表明,森林生态系统发生转变时,群落优势-多样性平衡发生突变。群落中优势种压力高,其丰度是第二种丰度的2.2 ~ 2.7倍。在稍微变化的森林中,该指标为1.1倍。相对于优势度指数和物种分布指数数据,物种相对丰度排序曲线是反映群落转变的更为敏感的指标。即使少量的人为结构进入森林生态系统,也会导致共生鸟类的相对丰度显著增加。在我们的研究中,1到2倍。在转换梯度上,共栖鸟类筑巢种数的绝对数量逐渐增加:30-33-36;专性共生种更强:0-2-7;群落同化指数增加1.5倍,为0.63 ~ 0.72 ~ 0.92。
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引用次数: 3
Ecological and toxicological assessment of the influence of the chemical enterprise on soil 化工企业对土壤影响的生态毒理学评价
Pub Date : 2019-03-06 DOI: 10.15421/031907
A. Krainiukov, I. Kryvytska
Technogenic pollution of the territory firstly leads to decrease of productivity of the land, since the main part of the technogenic loading, as a result of such pollution, assumes to the soil and vegetation. Soils those have been contaminated are characterized by changed structural and physical-and-chemical properties, which does not allow them to be used for economic purposes. To detect the technogenic loading on the soil as a result of the chemical plant operation, soil quality studies were carried out using the bio-testing method. The most informative data towards ecological danger of ecosystem pollution is the results of the determination of phytotoxicity for soil organisms. Such information can be obtained by an integral assessment of the biological usefulness of the habitat of organisms using the bio-testing method, which provides registration in controlled, standard conditions of the corresponding reactions of test organisms to the toxic effects of ecologically hazardous chemical compounds and their mixes. The following higher plants were selected as test cultures: Raphanus sativus L., Hordeum vulgare L. and Triticum aestivum L. As the test-response of higher plants, the energy of seed germination, the length of the sprout and the length of the root were taken into account. Four zones were selected for the research. The largest difference between the values of germination energy between control and experiment was observed on the results of a test-response to Hordeum vulgare L. in a sample of soil from zone Nr 4. The indicator of the toxic effect detected on the territory of zone Nr 4 - 43,64% reduction in the length of the roots relative to the control; 33.81% reduction in the length of the germs relative to the control based on the results of the test-response to Hordeum vulgare L. The soil sample from the zone Nr 1 showed no toxic properties in any test reaction (6.32% decrease in root length relative to control one, 5.68% reduction length of germs relative to the control one based on the results of the test reaction on Raphanus sativus L. Sample of soil from zone Nr 2 did not show toxicity (-45.26% decrease in root length relative to control one and 8.61% decrease in germination length relative to control one revealed by the results of the test-response to Raphanus sativus L. Soil samples from zone Nr 3 also showed no toxicity (3.83% decrease in root length relative to control and 11.02% reduction in germination length relative to control revealed by test-reaction results on Raphanus sativus L. The table value of the Student's criterion for the probability level is P = 0.5 and the number of measures of freedom 58 is 2.01. The calculation of the actual value of Student's criterion for the length of the roots (2.68) and the germs (2.05) showed an excess of the table value based on the results of the test-response on Hordeum vulgare L. in the soil sample from zone Nr 4, what confirms the presence of toxic properties of the soil. In order
土地的技术污染首先导致土地生产力的下降,因为这种污染导致的技术负荷的主要部分是土壤和植被。被污染的土壤的特征是结构和物理化学性质发生了变化,这就不允许它们用于经济目的。为了检测化工厂运行对土壤的技术负荷,采用生物试验方法对土壤质量进行了研究。关于生态系统污染的生态危害,最翔实的数据是土壤生物的植物毒性测定结果。这种信息可以通过使用生物测试方法对生物体栖息地的生物有用性进行综合评估来获得,该方法在受控的标准条件下登记测试生物体对生态有害化合物及其混合物的毒性作用的相应反应。选择高等植物Raphanus sativus L.、Hordeum vulgare L.和Triticum aestivum L.作为高等植物的试验响应,考虑种子萌发能量、芽长和根长。选择了四个区域进行研究。在第4区土壤样品中,对普通Hordeum vulgare L.的试验响应结果显示,对照与试验组的萌发能值差异最大。在n4 - 43区检测到的毒效指标显示,与对照相比,根长减少64%;1区土壤样品在所有试验反应中均未表现出毒性(根长较对照减少6.32%);减少5.68%的细菌相对于控制一个基于测试的结果反应萝卜l .土壤样本区Nr 2根长度下降未显示毒性(-45.26%相对于控制和降低8.61%发芽长度相对于测试响应的结果显示,控制一个萝卜l .土壤样本区Nr 3根长度下降还显示没有毒性(3.83%相对于控制和减少11.02%对Raphanus sativus L.试验反应结果揭示的相对于对照的萌发长度。学生的概率水平判据表值为P = 0.5,自由度58的测度数为2.01。根据nr4区土壤样品中对普通Hordeum vulgare L.的试验响应结果,对根长(2.68)和芽长(2.05)的Student标准的实际值进行了计算,结果显示超出了表值,证实了土壤存在毒性。为了评估土壤污染的风险,根据生长过程的抑制程度使用“土壤污染测度”指标,土壤污染系数表示土壤污染的定量特征,土壤污染系数根据生长过程的抑制程度进行区分。根据计算得到的数据,在样品Nr 4中,根据试验响应的结果,根系长度相对于对照的植物毒性因子或百分比减少为43.64%。按污染程度计算,土壤为中度污染,属于III类质量,污染程度为1,3。土壤样品№1、№2和№3显示没有毒性(А依据20),即它们没有污染,属于I类质量。因此,对于土壤质量的综合评价,除了化学指标外,建议使用综合指标,特别是土壤的植物毒性,它表征了生物栖息地的生物有用性。
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引用次数: 0
Trophic selectivity of litter saprophages (Diplopoda, Isopoda) in natural forests in the steppe conditions 草原条件下天然林凋落物腐菌(双足、等足目)的营养选择性
Pub Date : 2019-03-02 DOI: 10.15421/031904
A. Pokhylenko, O. Didur, Y. Kulbachko, Yuliia Ovchynnykova
The forest litter serves as a bridge between the above ground forest flora and fauna with the soil. Trophic activity of soil invertebrates affects the decomposition processes of plant organic residues and, thus, on the forest soils fertility. It is a natural mechanism that positively influences on maintenance and improvement of natural soils properties. The object of this study is the representatives of Diplopoda Rossiulus kessleri (Lohmander, 1927) and Isopoda Porcellio scaber (Latreille, 1804). The aim of the paper is to qualify the trophic preferences of the saprophages, as soil invertebrate representatives, of natural forest ecosystems in conditions of the steppe zone of Ukraine. To identify the ecological role of millipedes and woodlice as primary destructors of leaf litter, we made a field experiment studying their trophic preferences with the use of microcosms. Microcosms (containers) were filled with soil free from invertebrates; after it added a certain mixture of leaf litter and saprophages representatives. To analyze the decomposition rate of leaf litter the percentage of consumed litter was determined by calculating air-dry mass of leaf litter in the beginning and at the end of experiment. A regression model that reflects the trophic selectivity of saprophages for the following mixture components of such tree species as the common maple (Acer campestre (L.), little-leaved linden (Tilia cordata (Mill.), common oak (Quercus robur (L.), European white elm (Ulmus laevis (Pall.), European ash (Fraxinus excelsior (L.) is proposed. Among other regression model types (linear, quadratic, special cubic), permissible statistical estimates revealed a special cubic mathematical model and it allowed to conclude on the saprophages trophic selectivity. The special cubic regression model has a relatively high performance (the determination coefficient is 87.5 %) and adequately approximates empirical data with a significance level of less than 0.05. It was found out that common maple and European white elm litter is consumed in a greater quantity than any other litter from suggested. It is confirmed by their main effects coefficients in the regression equation. Thus, the saprophages trophic selectivity  persistence in natural forests under the conditions of the steppe zone has been proved. Consequently, the saprophages trophic activity increases with the presence of the maple and European white elm in natural forests, which makes expedient the use of these tree species, in the design of forest stands.
森林凋落物是地面上森林动植物与土壤之间的桥梁。土壤无脊椎动物的营养活动影响植物有机残留物的分解过程,从而影响森林土壤的肥力。这是一种对土壤自然性质的保持和改善有积极影响的自然机制。本研究的对象是Rossiulus kessleri (Lohmander, 1927)和Porcellio scaber (Latreille, 1804)等足类的代表。该论文的目的是合格的腐菌类的营养偏好,作为土壤无脊椎动物的代表,自然森林生态系统在乌克兰草原地带的条件。为了确定千足虫和木虱作为凋落叶主要破坏者的生态作用,我们利用微型群落研究了千足虫和木虱的营养偏好。microcosmos(容器)中装满了没有无脊椎动物的土壤;之后加入一定的凋落叶和腐殖体代表的混合物。为了分析凋落叶的分解速率,通过计算凋落叶在试验开始和结束时的空气干质量来确定凋落叶的消耗百分比。摘要建立了腐菌体对槭(Acer campestre, L.)、小叶椴树(Tilia cordata, Mill.)、栎(Quercus robur, L.)、欧洲白榆树(Ulmus laevis, Pall.)、欧洲白蜡树(Fraxinus excelsior, L.)混合组分营养选择性的回归模型。在其他回归模型类型(线性、二次、特殊三次)中,允许的统计估计揭示了一个特殊的三次数学模型,它可以得出腐菌体营养选择性的结论。特殊的三次回归模型具有较高的性能(决定系数为87.5%),能够充分逼近经验数据,显著性水平小于0.05。研究发现,普通枫树和欧洲白榆树凋落物的消耗量比其他任何凋落物都要大。由回归方程中的主要影响系数证实了这一点。因此,证明了草原带条件下天然林腐生菌营养选择性的持久性。因此,随着天然森林中枫树和欧洲白榆树的存在,腐生菌的营养活性增加,这使得在森林林分设计中使用这些树种是有利的。
{"title":"Trophic selectivity of litter saprophages (Diplopoda, Isopoda) in natural forests in the steppe conditions","authors":"A. Pokhylenko, O. Didur, Y. Kulbachko, Yuliia Ovchynnykova","doi":"10.15421/031904","DOIUrl":"https://doi.org/10.15421/031904","url":null,"abstract":"The forest litter serves as a bridge between the above ground forest flora and fauna with the soil. Trophic activity of soil invertebrates affects the decomposition processes of plant organic residues and, thus, on the forest soils fertility. It is a natural mechanism that positively influences on maintenance and improvement of natural soils properties. The object of this study is the representatives of Diplopoda Rossiulus kessleri (Lohmander, 1927) and Isopoda Porcellio scaber (Latreille, 1804). The aim of the paper is to qualify the trophic preferences of the saprophages, as soil invertebrate representatives, of natural forest ecosystems in conditions of the steppe zone of Ukraine. To identify the ecological role of millipedes and woodlice as primary destructors of leaf litter, we made a field experiment studying their trophic preferences with the use of microcosms. Microcosms (containers) were filled with soil free from invertebrates; after it added a certain mixture of leaf litter and saprophages representatives. To analyze the decomposition rate of leaf litter the percentage of consumed litter was determined by calculating air-dry mass of leaf litter in the beginning and at the end of experiment. A regression model that reflects the trophic selectivity of saprophages for the following mixture components of such tree species as the common maple (Acer campestre (L.), little-leaved linden (Tilia cordata (Mill.), common oak (Quercus robur (L.), European white elm (Ulmus laevis (Pall.), European ash (Fraxinus excelsior (L.) is proposed. Among other regression model types (linear, quadratic, special cubic), permissible statistical estimates revealed a special cubic mathematical model and it allowed to conclude on the saprophages trophic selectivity. The special cubic regression model has a relatively high performance (the determination coefficient is 87.5 %) and adequately approximates empirical data with a significance level of less than 0.05. It was found out that common maple and European white elm litter is consumed in a greater quantity than any other litter from suggested. It is confirmed by their main effects coefficients in the regression equation. Thus, the saprophages trophic selectivity  persistence in natural forests under the conditions of the steppe zone has been proved. Consequently, the saprophages trophic activity increases with the presence of the maple and European white elm in natural forests, which makes expedient the use of these tree species, in the design of forest stands.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85716192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The reaction of soybean symbiotic apparatus to losses of water content in leaves and roots, induced by continuous action of drought 干旱持续作用下大豆共生器官对叶片和根系水分损失的反应
Pub Date : 2019-03-02 DOI: 10.15421/031908
T. Mamenko
The reaction of the soybean symbiotic apparatus inoculated with Bradyrhizobium japonicum strains and Tn5 mutants, which were different in efficiency, was studied for the loss of water content in leaves and roots induced by prolonged drought, as well as the seed productivity of the formed symbiotic systems. To conduct researches were used microbiological, physiological, biochemical methods, gas chromatography and spectrophotometry. The objects of the study were selected symbiotic systems formed with the participation of soybean plants and strains B. japonicum 646 (active, virulent) and 604k (inactive, highly virulent), as well as Tn5-mutants – B1-20 (active, virulent) and 107 (low-active, virulent) obtained by the method of transposon mutagenesis in the department of symbiotic nitrogen fixation at the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Before sowing, sterilized with 70 % ethanol and washed under running water for 1 h, the seeds were inoculated with suspensions of nodules bacteria (the titre of the suspension was 108 cells in 1 ml). The combined model drought was created during 12 days by stopping watering of plants up to 40 % of full moisture content starting from the stage of two true leaves and gradual transfer of watering to 30 % of full moisture content in the stage of three true leaves and budding - the beginning of flowering. After the stopping of drought, the moisture content of the substrate was adjusted to 60 % of full moisture content (watering recovery) into the stage of mass flowering. Control plants were inoculated by rhizobium culture, as well as plants without inoculation, which grew for optimal watering. It was investigated that in symbiotic systems formed with the participation of soybean and the active strain B. japonicum (646) and Tn5-mutant (B1-20) there was no significant reduction in the water content of plants under drought conditions and the effective work of the symbiotic apparatus was recorded, which contributed to the preservation of seed productivity. Ineffective symbiotic systems observed significant losses in water content and inhibition of the process of nodulation (strain 604k) and nitrogen fixation (Tn5-mutants 107), which was accompanied by significant losses of soybean crop yields. As a result of the research, it was concluded that in soil-climatic conditions with insufficient rainfall and frequent droughts, effective symbiotic systems should be used, which will promote the optimal functioning of the symbiotic apparatus and preserve the seed productivity of soybeans by adaptive regulation of water balance and fixation of molecular nitrogen of the atmosphere. The study of the functioning of leguminous plants in symbiosis with strains of nodule bacteria is important for finding effective symbiotic systems that are able to realize their adaptive potential for the effects of stress factors, in particular drought. Effective symbiotic relationships are the main so
研究了接种效率不同的缓生根瘤菌株系和Tn5突变体的大豆共生装置对长期干旱诱导的叶片和根系水分损失以及形成的共生系统的种子产量的影响。采用微生物学、生理、生化、气相色谱、分光光度等方法进行研究。研究对象选择了乌克兰国家科学院植物生理与遗传研究所共生固氮部通过转座子诱变方法获得的大豆植株与日本白僵菌646(活性、强毒)和604k(无活性、强毒)参与形成的共生系统,以及tn5突变体- B1-20(活性、强毒)和107(低活性、强毒)。播种前用70%乙醇消毒,流水冲洗1 h,用结核菌悬浮液接种种子(悬浮液滴度为108细胞/ 1 ml)。联合干旱模型是在12天内通过从两个真叶阶段开始停止浇水到植物全部水分含量的40%,并在三个真叶和萌芽阶段逐渐转移到30%的全部水分含量-开花开始。干旱停止后,将基质含水率调整到满含水率的60%(复水),进入大量开花阶段。对照植株接种根瘤菌培养,对照植株不接种根瘤菌,以获得最佳浇水条件。结果表明,在大豆与日本芽孢杆菌(646)和tn5突变体(B1-20)组成的共生系统中,干旱条件下植物含水量没有显著降低,共生装置的有效工作有助于种子生产力的保存。无效的共生系统中,大豆的含水量和结瘤过程(604k菌株)以及固氮过程(tn5突变体107)均有显著损失,同时大豆产量也有显著损失。研究结果表明,在降雨不足、干旱频繁的土壤气候条件下,应采用有效的共生系统,通过自适应调节水分平衡和大气分子氮的固定,促进共生装置的最佳功能,保持大豆的种子生产力。研究豆科植物与结瘤菌菌株共生的功能,对于发现有效的共生系统具有重要意义,这些系统能够实现其对胁迫因素,特别是干旱影响的适应潜力。有效的共生关系是陆地生态系统固氮的主要来源,这将减少向土壤中添加化合物的需要,并提供额外的经济和环境优势。
{"title":"The reaction of soybean symbiotic apparatus to losses of water content in leaves and roots, induced by continuous action of drought","authors":"T. Mamenko","doi":"10.15421/031908","DOIUrl":"https://doi.org/10.15421/031908","url":null,"abstract":"The reaction of the soybean symbiotic apparatus inoculated with Bradyrhizobium japonicum strains and Tn5 mutants, which were different in efficiency, was studied for the loss of water content in leaves and roots induced by prolonged drought, as well as the seed productivity of the formed symbiotic systems. To conduct researches were used microbiological, physiological, biochemical methods, gas chromatography and spectrophotometry. The objects of the study were selected symbiotic systems formed with the participation of soybean plants and strains B. japonicum 646 (active, virulent) and 604k (inactive, highly virulent), as well as Tn5-mutants – B1-20 (active, virulent) and 107 (low-active, virulent) obtained by the method of transposon mutagenesis in the department of symbiotic nitrogen fixation at the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Before sowing, sterilized with 70 % ethanol and washed under running water for 1 h, the seeds were inoculated with suspensions of nodules bacteria (the titre of the suspension was 108 cells in 1 ml). The combined model drought was created during 12 days by stopping watering of plants up to 40 % of full moisture content starting from the stage of two true leaves and gradual transfer of watering to 30 % of full moisture content in the stage of three true leaves and budding - the beginning of flowering. After the stopping of drought, the moisture content of the substrate was adjusted to 60 % of full moisture content (watering recovery) into the stage of mass flowering. Control plants were inoculated by rhizobium culture, as well as plants without inoculation, which grew for optimal watering. It was investigated that in symbiotic systems formed with the participation of soybean and the active strain B. japonicum (646) and Tn5-mutant (B1-20) there was no significant reduction in the water content of plants under drought conditions and the effective work of the symbiotic apparatus was recorded, which contributed to the preservation of seed productivity. Ineffective symbiotic systems observed significant losses in water content and inhibition of the process of nodulation (strain 604k) and nitrogen fixation (Tn5-mutants 107), which was accompanied by significant losses of soybean crop yields. As a result of the research, it was concluded that in soil-climatic conditions with insufficient rainfall and frequent droughts, effective symbiotic systems should be used, which will promote the optimal functioning of the symbiotic apparatus and preserve the seed productivity of soybeans by adaptive regulation of water balance and fixation of molecular nitrogen of the atmosphere. The study of the functioning of leguminous plants in symbiosis with strains of nodule bacteria is important for finding effective symbiotic systems that are able to realize their adaptive potential for the effects of stress factors, in particular drought. Effective symbiotic relationships are the main so","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82668100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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Ecology and Noospherology
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