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Dynamics of protection antioxidant system of representatives of Amelanchier Medik. genus during ontogenesis under Steppe Pridniprov’e conditions 梅兰芝代表植物保护抗氧化系统的动态研究。草原pridniprove条件下个体发生过程中的属
Pub Date : 2018-06-10 DOI: 10.15421/031801
Y. Lykholat, N. Khromykh, L. Shupranova, T. Legostaeva, T. Lykholat, A. Onopa
Antioxidant reaction in leaves of the representatives of Amelanchier Medik. genus introduced in Steppe Pridniprov’e during vegetative period  is studied. Activity of bensidin-peroxidase (BPOD), guaiacol-peroxidase (GPOD) and catalase (CAT) in leaves of four kinds Amelanchier Medik is analysed. Specific features of dynamics of enzymes activity in leaves of representatives of Amelanchier Medik. genus are defined according to phases of seasonal development and in reply to action of hydrothermal stress. On the basis of the received results the wide range of interspecific variability of enzymes activity in leaves of Amelanchier kinds, introduced in new conditions of existance is shown. The studied kinds were characterised by high activity ВРОD in all vegetative season. The greatest activity in leaves of all kinds was shown in a phase of secondary growth, in the most adverse hydrothermal mode that testified to passage of adaptive processes in plants. It is established, that unlike A. ovalis in which leaves level of activity BPOD was equal enough, other kinds in process of ontogenesis have shown sharper differences in activity of enzyme. The difference was essential, especially at A. canadensis in which leaves in May and June sharp falling of activity in 4,0 and 2,9 times accordingly in comparison with April was noticed, and in July and August – sharp increase that testified to a stressful condition of plants. The opposite tendency is found out in dynamics of activity GPOD i CAT. In the beginning phases of active growth (April) at all kinds the highest activity of enzymes, but, since May and till the end of the supervision period was observed, activity sharply went down at all kinds Amelanchier. Essential activation GPOD in a phase of active growth can be connected with processes of lignification to an greater degree, rather than with protection against oxidative stress. Thus in leaves of A. ovalis and A. canadensis higher activity gvaiacol-peroxidase, than at A. spicata and A. florida was observed. Small increase of activity was observed in June and to an greater measure in three kinds: A. ovalis, A. florida і A. spicata. In leaves of A. canadensis activity increase was marked in August in comparison with the period from May till July. The analysis of dynamics of catalase activity in leaves of representatives Amelanchier Medik. has shown similitude as well as at gvaiacol-peroxidase: the highest level was observed in April, and further there was an essential recession of enzyme activity. Dynamics of catalase activity during vegetation has appeared the most similar at kinds of A. ovalis and A. canadensis. In leaves of A. florida unlike last kinds the small peak of activity in July was marked, and in August activity again went down on 21,8 % in comparison with July. A. spicata has shown essential increase of activity САТ in August in comparison with July in 6,4 times. Thus, found out different directions of activity changes of oxidoreductases specify in p
梅兰属代表性植物叶片的抗氧化反应。研究了pridniprove草原在营养期引种的属。分析了四种青菜叶片中苯并苷过氧化物酶(BPOD)、愈创木酚过氧化物酶(GPOD)和过氧化氢酶(CAT)的活性。梅兰属代表植物叶片酶活性动态的具体特征。属是根据季节发展的阶段和热液应力的作用来定义的。在已有结果的基础上,揭示了在新的生存条件下引入的梅兰属植物叶片酶活性的广泛种间变异。所研究的种类在整个生长季节都具有较高的活性ВРОD。所有类型的叶片在次生生长阶段表现出最大的活性,在最不利的水热模式下,证明了植物适应过程的通过。结果表明,与卵圆草叶片BPOD活性水平相当不同的是,在个体发生过程中,其他种类的叶片BPOD活性差异较大。这种差异是非常重要的,特别是加拿大芥,其叶片在5月和6月的活性比4月急剧下降了4,0和2,9倍,而在7月和8月则急剧增加,证明植物处于应激状态。GPOD在CAT中的活性动态则呈现相反的趋势。在活性生长初期(4月)各品种酶活性最高,但从5月开始至监测期结束,各品种酶活性急剧下降。活性生长阶段的必需活化GPOD可以在更大程度上与木质素化过程有关,而不是与抗氧化应激有关。因此,在卵圆槭和加拿大槭的叶片中观察到较高的gvaiacol-过氧化物酶活性。在6月观察到的活动略有增加,在三种类型的活动中有较大的增加:A.卵圆,A.佛罗里达和A. spicata。与5月至7月相比,8月加拿大桤木叶片活性明显增加。代表植物梅兰芝叶片过氧化氢酶活性动态分析。在gvaiacol-过氧化物酶方面也表现出相似之处:在4月份观察到最高水平,随后酶活性出现基本衰退。植物生长过程中过氧化氢酶活性的动态变化在卵圆蒿和加拿大蒿中表现得最为相似。与前几种不同的是,佛罗里达州叶片在7月出现了小高峰,8月再次下降,比7月下降了21.8%。8月spicata的活跃性增加САТ比7月增加了6.4倍。从而发现了参与抗氧化系统中氧化还原酶活性变化的不同方向,从而实现了不同种类的适应。Pridneprov草原条件下个体发生过程的属。梅兰叶片酶活性水平的变异。植被在生长过程中对环境条件的适应,在整体上表现出所研究种类的适应能力,以牺牲较高的抗氧化能力来抵消非生物因素不利作用下氧化应激的发展。
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引用次数: 1
Scientific aspects of supplying the population with transported water 为人口提供运输水的科学方面
Pub Date : 2018-06-05 DOI: 10.15421/031807
O. V. Zorina
The research was conducted with the purpose of giving the scientific evaluation of the prevalence of supplying the population with transported potable water, its quality, and reveals the problems of regulatory framework in this sphere with the aim to improve the legislation and develop preventive measures for prevention of morbidity of the population. In this research were used following methods: hygienic, chemical, standard searching, and method of expert evaluation. It was determined that according to the data for the 2016, 10 regions of Ukraine are supplied with transported water in tanker due to the absence or deficit of the water of potable quality (8 regions – on the south and south–east, 2 regions – on the west of Ukraine). In order of increasing of the number of human settlements, which are supplied with the transported potable water, mentioned regions can be put in the following row: Zaporizka > Dnipropetrovska > Mykolaivska > Odeska > Donetska > Lvivska > Kyrovogradska > Khersonska > Poltavska > Ivano–Frankivska. Herewith, in order of increasing of the number of population supplied with the transported water can be put in the following row: Mykolaivska > Kyrovogradska > Zaporizka > Odeska > Dnipropetrovska > Khersonska > Donetska > Lvivska > Poltavska > Ivano–Frankivska. Dynamics analysis of potable transported water availability in human settlements of selected regions of Ukraine found that transported potable water intended for drinking in 2009 was used in 11 regions, in 2014 has not been used in Vinnitska and Poltavska region, however, the transportation of potable water started in 2014–2016 in Lvivska region. Were identified problematic regions, where was observed an increase in supply of transported water in recent years: Zaporizka > Dnipropetrovska > Mykolaivska > Odeska > Donetska > Lvivska > Kyrovogradska. It has been established that as for today the quality of transported water is not controlled by the State supervisory authorities. The need of reglamentation of the quality and recommendations on the quantity of transported potable water in emergencies in SanPiN, what is topical issue for Ukraine, was identified. According to the requirements of European legislation the quality of such water must meet the standard for potable tap water (Directive 98/83/EU on water intended for human consumption). According to the data from scientific literature and personal research the minimum quantity of such water per capita in emergencies should be 10 liters a day (5 litres – for hygienic needs and 5 liters – for internal use). However, it is should be taken into account that the regime of limitation of water consumption in considerable heat and/or physical activity conditions should not be applied. Moreover, population should be supplied with enough quantity of potable water for all physiological needs of organism when opportunities are presented. The alternative to transported potable water under appropriate conditions is a usage of mobi
所得结果的科学新颖性在于确定了乌克兰运输饮用水在特定时间尺度上的供应规律。这是第一次根据饮用水的质量和运输给消费者的皮的种类对运输的水进行分类。这项研究的实际价值在于,有可能确定乌克兰饮用水短缺的地区,这些地区不是出于商业目的运输的,而且SanPiN 2.2.4-171-10“供人类消费的饮用水的卫生要求”的若干规定(及其新版本是为了实施用于人类消费的水的98/83/EU指令而制定的)。
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引用次数: 1
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Ecology and Noospherology
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