Ecology and Noospherology最新文献

{"title":"Characteristics of the toxic effect of imidacloprid on the state of Eisenia fetida (Annelida, Clitellata, Lumbricidae)","authors":"A. Huslystyi, V. Gasso, S. Yermolenko, V. Petrushevskyi","doi":"10.15421/032107","DOIUrl":"https://doi.org/10.15421/032107","url":null,"abstract":"Imidacloprid became the most sold neonicotinoid insecticide in the world in the 21st century. Due to their constant use, neonicotinoids are stored in soils, bottom deposits, and surface waters. It is known that neonicotinoids reveal toxicity for non-target species: annelid worms, insects, fish, birds, and mammals. Neonicotinoids exhibit reproductive toxicity, neuro-, hepato-, and genotoxicity for mammals. Earthworms are useful modeling organisms that are proposed to carry out toxicity tests. Eisenia fetida is considered a convenient and adequate species in toxicological studies. Analysis of available data shows that even low concentrations of imidacloprid caused protrusion of a belt, blackening, leakage of a cellar fluid, narrowing areas of the body with edema in segments, and dark body pigmentation in E. fetida. There are also biochemical changes. Low concentrations of imidacloprid (<0.2 mg/L) suppress the activity of cellulase. Imidacloprid also causes damage to DNA, production of reactive oxygen species, and alterations in antioxidant enzymes activity in E. fetida: inhibition of catalase, but activation of superoxide dismutase and glutathione-S-transferase. Imidacloprid reduces reproductive success in E. fetida, causing significant deformation of sperm, reducing the average number and size of cocoons and the success of birth. At concentrations ≥10 mg/kg of imidacloprid, the high mortality of worms makes it impossible for vermiculture. At 5 mg/kg of imidacloprid in plants remains for vermicomposting in seven weeks, the mortality rate of 25% of E. fetida individuals is fixed. The stereotypical escape reflex behavior in the worms was observed in relation to imidacloprid at a concentration of 1.32 mg/kg. At the same time, molecular and cellular mechanisms of toxic effects of imidacloprid on E. fetida are almost not studied and needed special attention with further research.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85708993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of forest vegetation on color, reflectivity and humus content in soils of northern variant ravines of the steppe zone of Ukraine","authors":"V. Gorban, M. S. Yakuba, A. Huslystyi","doi":"10.15421/032105","DOIUrl":"https://doi.org/10.15421/032105","url":null,"abstract":"Unique natural forests grow in the conditions of ravines of the steppe zone of Ukraine. Soil scientists have been researching the soils of ravines for more than 60 years. Despite long-term research, aspects of the genesis of specific ravine soils, which are reflected in their optical properties, are still virtually unexplored. Based on this, the aim of our work is to establish the characteristics of the influence of forest vegetation on color and reflectivity, as well as the closely related content of humus in the soils of the northern variant ravines of the steppe zone of Ukraine. Soil samples were taken from each genetic horizon of sections laid in the Glybokyy ravine (near the village of Andriivka, Novomoskovsk district, Dnipropetrovsk region). Soil color indices were determined by scanning soil samples followed by image analysis. The reflectivity of soils was investigated using a monochromator. The humus content in soils was determined by the standard method of wet oxidation of organic matter according to I. V. Tyurin. As a result of the performed researches it is established that the upper horizons of the soils of the Glybokyy ravine differ in the reduced values ​​of the indicators of the HSB, RGB and Lab systems, with depth their values ​​increase. The upper horizons of the ravine soils are characterized by reduced values ​​of brightness coefficients at wavelengths of 480, 650 and 750 nm, as well as the integrated brightness coefficient, with depth there is a gradual increase in their values. The color indicators of the RGB and Lab systems are the most successful for diagnosing and predicting the humus content in the soils of the ravine. Forest chernozems and forest-meadow soil of the ravine, which were formed under natural forest vegetation, are characterized by reduced values ​​of color indicators of HSB, RGB and Lab systems, reduced values ​​of brightness coefficients and increased humus content compared to chernozems, the genesis of which is related.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90363075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-resistant microbiota of water and soil ecosystems as risk factors for human health","authors":"T. Y. Matylonok, O. Pakhomov, N. Polishchuck","doi":"10.15421/032111","DOIUrl":"https://doi.org/10.15421/032111","url":null,"abstract":"Bacterial resistance to antibiotics is one of the three major health challenges of the 21st century. One of the most important reasons for the acquisition and spread of antibiotic resistance in the environment is the irrational and uncontrolled use of antibacterial drugs, not only for medical but also other purposes, and their improper disposal. The microbiome of aquatic and soil ecosystems is characterized by the acquisition of antibiotic resistance through mobile genetic elements, contact with antibacterial drugs and their residues, the action of heavy metals and environmental stress. Also, according to the literature, it is noted that the resistance of microorganisms to antibacterial drugs in the environment existed much earlier than in clinical strains. These facts can not help but worry, because antibiotic-resistant strains of the environment have an extremely negative impact on human health. Once in the human body with water and food, they significantly complicate and / or make it impossible to further treat life-threatening diseases. Also, antibacterial residues circulating in aquatic and soil ecosystems, entering the human body can cause cancer, allergic reactions or disruption of the natural intestinal microflora. These ecosystems are characterized by large-scale spread of antibiotic-resistant microorganisms, antibacterial drugs and their residues. The aim of our work was to analyze with the help of theoretical methods of scientific research the reasons for the acquisition and spread of antibiotic resistance among environmental microbiota, namely in aquatic and soil ecosystems. To determine the impact of antibiotic-resistant bacteria of these ecosystems on human health. We have found that antibacterial drugs, antibiotic-resistant strains and resistance genes are a particular problem for wastewater treatment. Antibiotics can provide a selective load, as the mechanisms that break them down can promote resilience and selectively enrich. Wastewater treatment plants can be a favorable factor for the horizontal transfer of genes and the development of bacterial polyresistance, and high-resistance genes can be preserved even after disinfection. Soil is also an important reservoir for antibiotic-resistant bacteria and resistance genes. Microorganisms are in a constant struggle for existence in this ecosystem and try to colonize the micro-scale with the most favorable for their ecotype habitat. Antibiotic-resistant soil bacteria are in close contact with other members of the microbiota, which in turn promotes the horizontal transfer of resistance genes, even between cells of different species or genera through genetic determinants. Conclusion: ecosystems are characterized by large-scale spread of antibiotic-resistant microorganisms, antibacterial drugs and their residues. Therefore, this problem should be properly addressed, as the presence of antibiotic-resistant microorganisms, antibacterial drugs and their residues in the environment can cause","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"220 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83640531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the antioxidant capacity and secondary metabolites accumulation in the fruits of rowan (Sorbus aucuparia L.) and some closely related species","authors":"Y. L. Lykholat, O. Didur, N. Khromykh, V. Davydov, Y. S. Borodai, K. V. Kravchuk, T. Lykholat","doi":"10.15421/032101","DOIUrl":"https://doi.org/10.15421/032101","url":null,"abstract":"The paper presents the results of studying fruits antioxidant properties of several species, previously combined into the genus Sorbus L. (Rosaceae family). Ripe fruits were collected in September 2020 from the plants of Cormus domestica (L.) Spach, Sorbus aucuparia L., Karpatiosorbus latifolia (Lam.) Sennikov & Kurtto and Torminalis glaberrima (Gand.) Sennikov & Kurtto from the collection of the Botanical Garden of the Oles Honchar Dnipro National University. Total phenolic content (TPC), total flavonoids content (TFC), ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) were determined both in the fruits peel and pulp. In the fruits of all species, higher values of the determined indicators were found in the fruit peel than in the pulp. Moderate and strong positive correlation was found between FRAP and TPC as well between FRAP and TFC both in peel and pulp of all studied species. At the same time, notable interspecific differences of the phytochemical traits were established, indirectly confirming the ambiguity of the taxonomy of the genus Sorbus L. According to the fruits antioxidant properties and secondary metabolites accumulation, species K. latifolia (broad-leaved whitebeam) and T. glaberrima (wild service-tree) are the most promising for cultivation in arid conditions of the central steppe of Ukraine.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"181 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80195293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-restoration of woody plants in the conditions of the Botanical Garden of Dnipro National University","authors":"M. Shamray, O. Pakhomov, A. Kabar","doi":"10.15421/032108","DOIUrl":"https://doi.org/10.15421/032108","url":null,"abstract":"It is impossible to overestimate the importance of parks in large industrial cities. The problem of preservation of local flora together with cultivation of introduced species is more actual. The species composition of seed self-restoration of woody plants in the forest park and park parts of the Botanical Garden of Oles Honchar Dnipro National University was analyzed in order to determine the ratio of introduced and autochthonous species and whether the introduced species pose a threat to aboriginal flora. Quantitative and qualitative state of seed self-restoration determined. As a result of the research it was established that in the forest-park and park zones of the Botanical Garden artificial stands are capable of forming a sufficient number of viable undergrowth of autochthonous and introduced species, among which mainly aboriginal species dominate. The ratio of indigenous species to introduced ones in different parts of the park and forest park is 57–76 % and 24–43 %, respectively. There is no special competition between indigenous and introduced species. Each species of woody species has adapted well to the conditions of the ecotope and to the conditions of its place of growth. Bioecological features of wood species in the plantations are not an obstacle to the development of the internal space of the ecotope, but 43 % of self-regenerating woody plants in trial area 1 are introduced species, so further observation is needed.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80638244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of seasonal climatic factors on the dynamics of birds interactions with linden consortia","authors":"O. Ponomarenko","doi":"10.15421/032006","DOIUrl":"https://doi.org/10.15421/032006","url":null,"abstract":"The article is devoted to the bird communities in individual linden consortia (Tilia cordata Mill.) of the linden-ash oak forests. This work material was collected during different seasons of the 2009–2017 years in a linden-ash oak grove on the test plot No. 209 of the ecological profile of the NSC «Bel'gard Prisamar`e International Biospheric stationary», Novomoskovsk district, Dnepropetrovsk region. The individual consortia of 145 examples of three age conditions oak trees (virgins – virg, young generative – gl, mature and old generative individuals – g2-g3) has been investigated. Studies have shown that birds are actively involved in consortia of linden for most of the year. Meroconsortia of linden generative organs are attractive to birds during the growing season and in the cold season. In summer, the consortia of linden in terms of species composition of birds is inferior to the consortia of oak about 2 times. The same trend is observed in DTB and DMB. Only 2 species of birds participate in the consortia of the virgin linden. The consortia of young generative linden consists of only 4 species of birds, but DTB increases almost 30 times. The consortia of mature and old generative linden acquires a sufficient species composition – 14 species of birds. Interactions of birds with virginal linden are stochastic in autumn. Only 2 species of birds participate in the consortia of virgin linden in autumn. The consortia of young generative linden consists of 5 species (more than in summer). The old generative linden has a depression of consortia interactions of birds. Their volume is reduced by half compared to summer. Specialized consumers of linden nuts remain in the consortia of old generative linden mainly. Birds have very low DTB and DMB rates in consortia of all linden age groups in winter. Instead, the species composition of birds increases in the consortia of generative groups of linden in winter. The system of consortia interactions of birds is not intensive, but stable on the linden tree in winter. The volume of interactions of birds with a linden tree essentially increases in the spring. This trend is typical for trees with a dense crown. The number of consort birds is higher than in summer in consortia of virgin and young generative linden. DTB is five times higher in virgin linden than in summer. The participation of birds in the consortia of young generative linden is also greater than in summer. We believe that this is due to the fact that linden begins to grow earlier than other trees in the upper tier. The old generative linden is one of the main feeding grounds for birds in the spring. Linden, like common oak, is much more interesting for birds in spring and summer in contrast to field maple and ash. Linden first supports the system of trophic connections, and then topical in most seasons of the year. Linden forms stable groups of birds at a young generative stage during the year. Linden creates an environment for birds to live in t","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84440750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaccumulation of heavy metals in the dice snake Natrix tessellata (Reptilia, Colubridae) from ecosystems of different anthropogenic loads in modern conditions of the steppe Dnieper area","authors":"S. Yermolenko, V. Gasso, A. Hagut, I. Hasso, V. A. Spirina","doi":"10.15421/031919","DOIUrl":"https://doi.org/10.15421/031919","url":null,"abstract":"We studied the peculiarities of bioaccumulation and distribution of heavy metals (cadmium, lead, zinc and copper) in the bone tissue, liver and kidneys of the dice snake Natrix tessellata (Laurenti, 1768) inhabited ecosystems under different anthropogenic loads in 2015-2016. Mature individuals were caught in anthropogenically transformed ecosystems of the sanitary-protective zone of the Dniprovska Thermal Power Plant (SPZ DTPP) (48.402°N, 35.111°E, n = 7), coastal ecosystems of the National Nature Park «Velykyi Luh» (47.443°N, 35.149°E, n = 8) and in the Maiorka gully ecosystems (48.260°N, 35.170°E, n = 7). The gross content of metals in the organs of N. tessellata was determined after their dry ashing in a muffle furnace. Determination of the trace elements was performed by atomic absorption spectrophotometry. Among the studied trace elements, zinc has the highest concentrations in the liver, kidneys and bones of N. tessellata. The kidneys and liver are characterized by such amount of concentrations: Zn> Cu> Pb> Cd, but bone tissue has the following ratio Zn> Pb> Cu> Cd. The highest concentration of cadmium is found in the kidneys, and the lowest one – in the bones of snakes. There are the highest contents of lead and copper in the snakes’ liver, but the lowest in the kidneys and bones. The lead bioaccumulations in organs of snakes from different ecosystems are dissimilar. Zinc content in bones is higher than in kidneys and liver, but the lowest concentration is found in the kidneys. It has been found that the highest contents of toxic metals (Cd, Pb) were in the kidneys and liver of the snakes dwelled in the SPZ DTPP ecosystems in comparison with both studied natural ecosystems. In addition, snakes caught in anthropogenically transformed ecosystems of SPZ DTPP are characterised by increased zinc content in all studied organs and enlarged copper concentration in the kidneys. Considering the obtained results on the metals bioaccumulation, the dice snake can be considered as a potential bio-indicator of environmental pollution by heavy metals.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73143236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hardware – wheat pests as elements of the species consortium","authors":"T. Kolombar","doi":"10.15421/031918","DOIUrl":"https://doi.org/10.15421/031918","url":null,"abstract":"Annually in the world, insect pests kill 10–15 % of the crop yield, which in the face of increasing humanity creates a global problem for food security. For the territory of Ukraine in some years, the loss of wheat yields reach 25 % and, in the face of this tendency, real risks to the security of the state are created. Considering that the agrarian trend of the state development has been increasing in recent years, the development of grain-adapted to the ecological and climatic conditions of Ukraine is a priority direction of the development of domestic agriculture. And the development of new environmentally friendly methods of controlling pests of wheat using parasitic organisms will become a fundamentally new and pressing issue in the development of organic production in the country. The general list of pest species that can cause serious wheat yield and forage reduction in Ukraine includes more than 230 names, but only 18 species are able toentail the biggest losses. Among them belong to the order Coleoptera. One insect species is usually a host of several species of mites, nematodes and up to ten species of gregarines and microsporidia. Unfortunately, the taxonomic composition of parasites of phytophagous pets of wheat in Ukraine has not been thoroughly investigated. We studied the interrelation between parasites of 4 phytophagous insects of wheat as well as of 14 insect species found in granaries. The study of the species composition of parasitic organisms of wheat and grain pests is based on the study of the elementary part of the consortium ecosystem, where they act as second order consortia. Phytophagous parasites of wheat and collar pests of cereals, as second-order consorts, have been fragmented worldwide. The purpose of this work is to determine the species composition of gregarine as a component of wheat consortium for their further use in the creation of integrated methods of protection of wheat and products of its processing. As a result, 28 species of gregarines were registered, which belong to seven families (Didymophyidae, Ophryocystidae, Hirmocystidae, Lipotrophidae, Stylocephalidae, Actinocephalidae and Gregarinidae). On the average, 1–2 species of gregarines parasitize in one pest species. 7–10 species of parasites were identified in some insects of the family Tenebrionidae (Tenebrio molitor Linnaeus, 1758 and Tribolium confusum Jacquelin du Val, 1863). Each presented parasite is characteristic of 1–2 species of pests, but there are species of gregarines that infect up to four species of model species, such as Gregarina cuneata Stein, 1848. The largest number of individuals who are infested with gregarines belong to such families as Tenebrionidae and Dermestidae (38,6 % and 36,0 % of the total number of species, respectively). On pests – phytophages parasitizes about 8 species of gregarine families Stylocephalidae, Gregarinidae, Actinocephalidae. For barns pests are recorded barnacles of all 7 families.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"143 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74865366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of the structural-aggregate composition of ordinary chernozems under the steppe and forest vegetation","authors":"V. Gorban', A. Boloban","doi":"10.15421/031913","DOIUrl":"https://doi.org/10.15421/031913","url":null,"abstract":"The structural-aggregate composition is an important soil characteristic, which reflects the features of the genesis, condition and regimes of soils. The structural and aggregate composition of ordinary chernozems under zonal steppe vegetation and under artificial forest plantations from white acacia and common oak in the conditions of the Prisamaría was studied. As a result, it was found that in all the studied soils the greatest content falls on the fraction 2–1 mm in size. In ordinary chernozems in the upper horizons, under the stands of white acacia and common oak, an increase in the content of this agronomically valuable fraction is observed. Also in ordinary chernozem under forest stands, an increase in the value of the structural coefficient is observed compared with ordinary chernozem under steppe vegetation. In ordinary chernozem the most favorable effect of the growth of white acacia was found in horizon H, and the growth of ordinary oak in horizon H2, in which the highest values ​​of the structural coefficient were found. In the studied ordinary chernozems under the steppe and forest vegetation a predominance of the water-resistant fraction of size <0.25 mm is observed. The positive effect of white acacia and common oak plantations on the water resistance of aggregates of common chernozem in the upper horizons is manifested in a decrease in the content of the fraction of size <0.25 mm compared to ordinary chernozem. In ordinary chernozems under steppe vegetation the most favorable conditions for the formation of agronomically valuable fractions during dry sieving are characteristic of the lower horizons and for chernozems under forest stands in the upper horizons. In all the studied soils the highest content of agronomically valuable fractions during wet sieving was found in the upper horizons, but there are more of them in chernozems under forest stands. The highest content of agronomically valuable fractions in all studied soils is observed in the lower horizons and their highest content in the upper horizons is found in ordinary chernozems under the steppe vegetation. The highest values ​​of the water resistance criterion are characteristic of the lower horizons of the studied chernozems. The maximum values ​​of this criterion were found in ordinary chernozem under oak plantation, and the smallest – under acacia plantation. The growth of forest plantations on ordinary chernozems leads to an improvement in their structural-aggregate composition.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84888961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Destruction of toluene and xylene by sulfatе-reducing bacteria","authors":"N. Verkholiak, T. Peretyatko","doi":"10.15421/031916","DOIUrl":"https://doi.org/10.15421/031916","url":null,"abstract":"As a result of human activity aromatic hydrocarbons enter the environment in large quantities, contaminating it. Dropping of insufficiently treated wastewater drains considerably decrease the quality of water. Quite effective biological methods of purification of contaminated environment are the usage of microorganisms. Prospective microorganisms for sewage treatment are sulfate-reducing bacteria. The purpose of the work was to investigate the ability of sulfate-reducing bacteria to use xylene and toluene as a source of carbon under different cultivation conditions.  The study objects were sulfate-reducing bacteria Desulfotomaculum AR1 and Desulfovibrio desulfuricans Ya-11. The biomass of bacteria was determined turbidimetrically, the content of sulfate ion and hydrogen sulfide – photometrically in the culture fluid. The content of fumarate was determined by the method of high-performance liquid chromatography. The ability of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria to use toluene and xylene as the sole source of carbon and energy has been established. In the toluene environment, a better growth of bacteria was observed. A comparison was made between the efficiency of the reduction of sulfate ions and the growth of bacteria in the control medium and the medium with aromatic compounds. The efficiency of sulfate ions reduction was sufficiently high in the control medium, whereas in the toluene/xylene media the efficiency of sulfate utilization and hydrogen sulfide accumulation was lower compared to the control parameters. The growth of Desulfotomaculum AR1 and D. desulfuricans Ya-11 sulfate-reducing bacteria was investigated in xylene and toluene media in the presence/absence of fumarate. According to the results of the studies, the best growth of the tested bacteria was observed in the medium with aromatic compounds in the presence of fumarate and sulfate ion. Efficiency of use of fumarate in the medium with toluene with bacteria Desulfotomaculum AR1 was more than 90 %. Fumarate can be used by sulfate-reducing bacteria as a source of carbon, donor and acceptor of electrons. Fumarate is most likely to inhibit sulfate reduction in Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria, as indicated by studies showing that sulfate reduction efficiency in lactate, fumarate, and sulfate ion media was half that of control. Sulfate-reducing bacteria are capable to anaerobically degrade aromatic hydrocarbons in the presence of sulfate ions as terminal electron acceptors. The ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to use the aromatics of the BTEХ – toluene, xylene and sulfate-ions, makes them promising at the biological purification step of contaminated wastewater from these pollutants.","PeriodicalId":11457,"journal":{"name":"Ecology and Noospherology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73340846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}